SK287488B6 - Spôsob rekombinantnej produkcie heterológneho polypeptidu pomocou peptidu s autoproteolytickou aktivitou - Google Patents

Spôsob rekombinantnej produkcie heterológneho polypeptidu pomocou peptidu s autoproteolytickou aktivitou Download PDF

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Publication number
SK287488B6
SK287488B6 SK185-2002A SK1852002A SK287488B6 SK 287488 B6 SK287488 B6 SK 287488B6 SK 1852002 A SK1852002 A SK 1852002A SK 287488 B6 SK287488 B6 SK 287488B6
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SK
Slovakia
Prior art keywords
polypeptide
cleavage
leu
pro
autoprotease
Prior art date
Application number
SK185-2002A
Other languages
English (en)
Slovak (sk)
Other versions
SK1852002A3 (en
Inventor
G�Nter Stempfer
J�Rg Windisch
Franz Knauseder
Original Assignee
Sandoz Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sandoz Ag filed Critical Sandoz Ag
Publication of SK1852002A3 publication Critical patent/SK1852002A3/sk
Publication of SK287488B6 publication Critical patent/SK287488B6/sk

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Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52Cytokines; Lymphokines; Interferons
    • C07K14/54Interleukins [IL]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/06Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/20Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/50Fusion polypeptide containing protease site
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/70Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
SK185-2002A 1999-08-09 2000-08-07 Spôsob rekombinantnej produkcie heterológneho polypeptidu pomocou peptidu s autoproteolytickou aktivitou SK287488B6 (sk)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT136799 1999-08-09
PCT/EP2000/007643 WO2001011057A1 (fr) 1999-08-09 2000-08-07 Production de proteines

Publications (2)

Publication Number Publication Date
SK1852002A3 SK1852002A3 (en) 2002-07-02
SK287488B6 true SK287488B6 (sk) 2010-11-08

Family

ID=3512357

Family Applications (1)

Application Number Title Priority Date Filing Date
SK185-2002A SK287488B6 (sk) 1999-08-09 2000-08-07 Spôsob rekombinantnej produkcie heterológneho polypeptidu pomocou peptidu s autoproteolytickou aktivitou

Country Status (21)

Country Link
US (2) US6936455B1 (fr)
EP (1) EP1200604B1 (fr)
JP (1) JP4820512B2 (fr)
KR (1) KR20020026366A (fr)
CN (1) CN1230543C (fr)
AT (1) ATE335826T1 (fr)
AU (1) AU775662B2 (fr)
CA (1) CA2379571C (fr)
CZ (1) CZ302366B6 (fr)
DE (1) DE60029968T2 (fr)
ES (1) ES2269173T3 (fr)
HK (1) HK1047128B (fr)
HU (1) HU230231B1 (fr)
IL (1) IL147456A0 (fr)
MX (1) MXPA02001424A (fr)
NO (1) NO328339B1 (fr)
NZ (1) NZ516869A (fr)
PL (1) PL200225B1 (fr)
SK (1) SK287488B6 (fr)
TR (1) TR200200253T2 (fr)
WO (1) WO2001011057A1 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1200603B1 (fr) * 1999-08-09 2014-12-24 Sandoz AG Production de proteines par clivage autoproteolytique
PL3219312T3 (pl) 2004-03-30 2019-07-31 Relypsa, Inc. Polimery wiążące jon i ich zastosowania
JP2008538897A (ja) * 2005-04-26 2008-11-13 サンド・アクチエンゲゼルシヤフト 融合タンパク質の自己タンパク質分解切断による組換えタンパク質の産生
GB0508435D0 (en) * 2005-04-26 2005-06-01 Sandoz Ag Organic compounds
AU2011253661B2 (en) * 2005-04-26 2013-06-13 Boehringer Ingelheim Rcv Gmbh & Co Kg Production of recombinant proteins by autoproteolytic cleavage of a fusion protein
GB0508434D0 (en) * 2005-04-26 2005-06-01 Sandoz Ag Organic compounds
US10526423B2 (en) 2010-08-31 2020-01-07 Amano Enzyme Inc. Eggshell membrane solubilization method using enzymes
EP2684951A1 (fr) 2012-07-13 2014-01-15 Sandoz Ag Procédé de production d'une protéine recombinante d'intérêt
EP2746390A1 (fr) 2012-12-19 2014-06-25 Sandoz Ag Procédé de production d'une protéine recombinante d'intérêt
EP2746391A1 (fr) 2012-12-19 2014-06-25 Sandoz Ag Procédé de production d'une protéine recombinante d'intérêt
UY35874A (es) 2013-12-12 2015-07-31 Novartis Ag Un proceso para la preparación de una composición de proteínas pegiladas
RU2619217C1 (ru) * 2015-12-04 2017-05-12 Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГБУ "ГосНИИгенетика") Температурочувствительный мутантный интеин для нерастворимой экспрессии предшественника целевого белка
CN114539425B (zh) * 2022-02-25 2023-04-28 湖南中晟全肽生化有限公司 一种提高线性多肽生物表达的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4743679A (en) * 1986-02-24 1988-05-10 Creative Biomolecules, Inc. Process for producing human epidermal growth factor and analogs thereof
EP0321973B1 (fr) * 1987-12-23 1993-11-03 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Expression de la protéase P2A de HRV2 codée par un virus
MX219629B (es) * 1997-04-25 2004-03-30 Sembiosys Genetics Inc Metodo para separar proteinas de fusion
DE59812768D1 (de) * 1997-08-22 2005-06-09 Roche Diagnostics Gmbh Autokatalytisch aktivierbare zymogene vorstufen von proteasen und deren verwendung
WO1999010483A2 (fr) 1997-08-22 1999-03-04 Roche Diagnostics Gmbh Precurseurs de proteases activables par voie autocatalytique et leur utilisation

Also Published As

Publication number Publication date
KR20020026366A (ko) 2002-04-09
DE60029968D1 (de) 2006-09-21
CZ2002481A3 (cs) 2002-05-15
CN1369014A (zh) 2002-09-11
HUP0202519A2 (en) 2002-10-28
CN1230543C (zh) 2005-12-07
CA2379571C (fr) 2011-03-29
HUP0202519A3 (en) 2004-07-28
AU775662B2 (en) 2004-08-12
CZ302366B6 (cs) 2011-04-13
NO328339B1 (no) 2010-02-01
PL353101A1 (en) 2003-10-20
NO20020633L (no) 2002-04-05
JP2003506093A (ja) 2003-02-18
US20050186564A1 (en) 2005-08-25
NZ516869A (en) 2004-01-30
SK1852002A3 (en) 2002-07-02
PL200225B1 (pl) 2008-12-31
AU6993300A (en) 2001-03-05
HU230231B1 (hu) 2015-10-28
TR200200253T2 (tr) 2002-05-21
EP1200604B1 (fr) 2006-08-09
MXPA02001424A (es) 2002-08-12
HK1047128A1 (en) 2003-02-07
HK1047128B (zh) 2007-06-08
US6936455B1 (en) 2005-08-30
NO20020633D0 (no) 2002-02-08
CA2379571A1 (fr) 2001-02-15
DE60029968T2 (de) 2006-12-21
ATE335826T1 (de) 2006-09-15
EP1200604A1 (fr) 2002-05-02
IL147456A0 (en) 2002-08-14
WO2001011057A1 (fr) 2001-02-15
JP4820512B2 (ja) 2011-11-24
ES2269173T3 (es) 2007-04-01

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