SK282611B6 - Spôsob výroby L-aminokyselín - Google Patents
Spôsob výroby L-aminokyselín Download PDFInfo
- Publication number
- SK282611B6 SK282611B6 SK235-98A SK23598A SK282611B6 SK 282611 B6 SK282611 B6 SK 282611B6 SK 23598 A SK23598 A SK 23598A SK 282611 B6 SK282611 B6 SK 282611B6
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- Slovakia
- Prior art keywords
- gene
- dna
- pps
- ala
- plasmid
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP22156195 | 1995-08-30 | ||
| JP20086096A JP4032441B2 (ja) | 1995-08-30 | 1996-07-30 | Ｌ−アミノ酸の製造方法 |
| PCT/JP1996/002399 WO1997008333A1 (fr) | 1995-08-30 | 1996-08-27 | Procede de production d'acides amines levogyres |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| SK23598A3 SK23598A3 (en) | 1998-09-09 |
| SK282611B6 true SK282611B6 (sk) | 2002-10-08 |
Family
ID=26512437
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SK235-98A SK282611B6 (sk) | 1995-08-30 | 1996-08-27 | Spôsob výroby L-aminokyselín |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US6319696B1 (zh) |
| EP (1) | EP0877090B1 (zh) |
| JP (1) | JP4032441B2 (zh) |
| CN (1) | CN1077139C (zh) |
| DE (1) | DE69635493T2 (zh) |
| DK (1) | DK0877090T3 (zh) |
| HU (1) | HU224895B1 (zh) |
| PL (1) | PL183472B1 (zh) |
| SK (1) | SK282611B6 (zh) |
| WO (1) | WO1997008333A1 (zh) |
Families Citing this family (94)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5985617A (en) * | 1997-02-18 | 1999-11-16 | Liao; James C. | Microorganisms and methods for overproduction of DAHP by cloned PPS gene |
| WO2000056859A1 (fr) * | 1999-03-19 | 2000-09-28 | Ajinomoto Co., Inc. | Procede de production des l-aminoacides |
| JP2003204783A (ja) * | 1999-07-19 | 2003-07-22 | Ajinomoto Co Inc | 発酵法による目的物質の製造法 |
| JP4362959B2 (ja) * | 2000-08-24 | 2009-11-11 | 味の素株式会社 | 塩基性アミノ酸の製造方法 |
| DE10045497A1 (de) * | 2000-09-13 | 2002-03-28 | Degussa | Neue für das ppsA-Gen kodierende Nukleotidsequenzen |
| DE10063314A1 (de) * | 2000-12-20 | 2002-07-04 | Degussa | Neue für das ilvE-Gen kodierende Nukleotidsequenzen |
| JP2002209596A (ja) * | 2001-01-19 | 2002-07-30 | Ajinomoto Co Inc | Ｌ−アミノ酸の製造法 |
| RU2229513C2 (ru) | 2001-11-23 | 2004-05-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" | Способ получения l-аминокислот, штамм escherichia coli - продуцент l-аминокислоты (варианты) |
| CN1615359B (zh) | 2001-11-23 | 2010-04-28 | 味之素株式会社 | 利用埃希杆菌属细菌生产l-氨基酸的方法 |
| RU2244007C2 (ru) * | 2002-02-27 | 2005-01-10 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" | Способ получения l-треонина, штамм escherichia coli - продуцент треонина (варианты) |
| JP4631706B2 (ja) | 2003-09-30 | 2011-02-16 | 味の素株式会社 | 発酵液からのコハク酸の精製方法 |
| KR101142885B1 (ko) * | 2003-12-15 | 2012-05-10 | 씨제이제일제당 (주) | 트립토판 생합성 관련 변이유전자를 함유한 대장균 변이주및 이를 이용한 트립토판 제조방법 |
| DE102004003410A1 (de) * | 2004-01-23 | 2005-08-25 | Degussa Ag | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von Stämmen der Familie Enterobacteriaceae |
| RU2288264C2 (ru) * | 2004-02-12 | 2006-11-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) | Бактерия, принадлежащая к роду escherichia - продуцент l-треонина и способ получения l-треонина |
| US7482140B2 (en) | 2004-06-15 | 2009-01-27 | Ajinomoto Co., Inc. | L-tyrosine-producing bacterium and a method for producing L-tyrosine |
| WO2006001379A1 (ja) * | 2004-06-25 | 2006-01-05 | Kyowa Hakko Kogyo Co., Ltd. | ジペプチドの製造法 |
| EP1976994A1 (en) * | 2006-01-26 | 2008-10-08 | Ajinomoto Co., Inc. | A method for producing an l-amino acid using a bacterium of enterobacteriaceae family with attenuated expression of the aldh gene |
| JP2009118740A (ja) * | 2006-03-03 | 2009-06-04 | Ajinomoto Co Inc | Ｌ−アミノ酸の製造法 |
| WO2007119574A2 (en) | 2006-03-23 | 2007-10-25 | Ajinomoto Co., Inc. | A method for producing an l-amino acid using bacterium of the enterobacteriaceae family with attenuated expression of a gene coding for small rna |
| JP2009153382A (ja) | 2006-03-30 | 2009-07-16 | Ajinomoto Co Inc | メタノール資化性細菌を用いたカルボン酸の製造法 |
| EP2007873B1 (en) * | 2006-04-18 | 2015-11-18 | Ajinomoto Co., Inc. | A METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY WITH ATTENUATED EXPRESSION OF THE sfmACDFH-fimZ CLUSTER OR THE fimZ GENE |
| JP2009165355A (ja) | 2006-04-28 | 2009-07-30 | Ajinomoto Co Inc | Ｌ−アミノ酸を生産する微生物及びｌ−アミノ酸の製造法 |
| EP2035569A1 (en) * | 2006-06-01 | 2009-03-18 | Ajinomoto Co., Inc. | A method for producing an l-amino acid using a bacterium of the enterobacteriaceae family with attenuated expression of the rcsa gene |
| RU2337961C2 (ru) * | 2006-07-04 | 2008-11-10 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) | СПОСОБ ПОЛУЧЕНИЯ L-ТРЕОНИНА С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ, ПРИНАДЛЕЖАЩЕЙ К РОДУ Escherichia, В КОТОРОЙ ИНАКТИВИРОВАН ОПЕРОН rspAB |
| CN1986777B (zh) * | 2006-07-11 | 2010-09-08 | 湖北大学 | 活体催化工程菌及利用α-酮酸生产L型非天然疏水性氨基酸的方法 |
| JP5407858B2 (ja) * | 2006-07-19 | 2014-02-05 | 味の素株式会社 | 腸内細菌科の細菌を用いたｌ−アミノ酸の製造方法 |
| RU2006129690A (ru) | 2006-08-16 | 2008-02-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) | СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТЫ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИЙ СЕМЕЙСТВА Enterobacteriaceae, В КОТОРОЙ ОСЛАБЛЕНА ЭКСПРЕССИЯ ГЕНА ydiN, ГЕНА ydiB ИЛИ ИХ КОМБИНАЦИИ |
| JP2010017081A (ja) * | 2006-10-10 | 2010-01-28 | Ajinomoto Co Inc | Ｌ−アミノ酸の製造法 |
| JP2010017082A (ja) * | 2006-10-10 | 2010-01-28 | Ajinomoto Co Inc | Ｌ−アミノ酸の製造法 |
| WO2008072761A2 (en) * | 2006-12-11 | 2008-06-19 | Ajinomoto Co., Inc. | Method for producing an l-amino acid |
| RU2006143864A (ru) | 2006-12-12 | 2008-06-20 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) | СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ СЕМЕЙСТВА ENTEROBACTERIACEAE, В КОТОРОЙ ОСЛАБЛЕНА ЭКСПРЕССИЯ ГЕНОВ cynT, cynS, cynX, ИЛИ cynR, ИЛИ ИХ КОМБИНАЦИИ |
| JP2010041920A (ja) | 2006-12-19 | 2010-02-25 | Ajinomoto Co Inc | Ｌ−アミノ酸の製造法 |
| RU2006145712A (ru) * | 2006-12-22 | 2008-06-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) | Способ получения l-аминокислот методом ферментации с использованием бактерий, обладающих повышенной способностью к утилизации глицерина |
| CN101627110B (zh) | 2007-01-22 | 2014-08-13 | 味之素株式会社 | 生产l-氨基酸的微生物和l-氨基酸的生产方法 |
| JP2010088301A (ja) | 2007-02-01 | 2010-04-22 | Ajinomoto Co Inc | Ｌ−アミノ酸の製造法 |
| JP2010110216A (ja) | 2007-02-20 | 2010-05-20 | Ajinomoto Co Inc | Ｌ−アミノ酸または核酸の製造方法 |
| JP2010110217A (ja) | 2007-02-22 | 2010-05-20 | Ajinomoto Co Inc | Ｌ−アミノ酸生産菌及びｌ−アミノ酸の製造法 |
| DE102007051024A1 (de) | 2007-03-05 | 2008-09-11 | Evonik Degussa Gmbh | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von Stämmen der Familie Enterobacteriaceae |
| JP2010130899A (ja) | 2007-03-14 | 2010-06-17 | Ajinomoto Co Inc | Ｌ−グルタミン酸系アミノ酸生産微生物及びアミノ酸の製造法 |
| EP1975241A1 (de) | 2007-03-29 | 2008-10-01 | Evonik Degussa GmbH | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von verbesserten Stämmen der Familie Enterobacteriaceae |
| CN101939412B (zh) | 2007-09-04 | 2016-01-20 | 味之素株式会社 | 生产氨基酸的微生物以及氨基酸的生产方法 |
| DE102007044134A1 (de) | 2007-09-15 | 2009-03-19 | Evonik Degussa Gmbh | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von verbesserten Stämmen der Familie Enterobacteriaceae |
| RU2396336C2 (ru) | 2007-09-27 | 2010-08-10 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) | СПОСОБ ПОЛУЧЕНИЯ АМИНОКИСЛОТ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ, ПРИНАДЛЕЖАЩЕЙ К РОДУ Escherichia |
| DE102007052270A1 (de) | 2007-11-02 | 2009-05-07 | Evonik Degussa Gmbh | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von verbesserten Stämmen der Familie Enterobacteriaceae |
| EP2060636A1 (de) | 2007-11-14 | 2009-05-20 | Evonik Degussa GmbH | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von verbesserten Stämmen der Familie Enterobacteriaceae |
| JP2011067095A (ja) | 2008-01-10 | 2011-04-07 | Ajinomoto Co Inc | 発酵法による目的物質の製造法 |
| EP2248906A4 (en) | 2008-01-23 | 2012-07-11 | Ajinomoto Kk | PROCESS FOR THE PREPARATION OF L-AMINO ACID |
| JP5217780B2 (ja) * | 2008-02-08 | 2013-06-19 | 味の素株式会社 | Ｌ−アミノ酸を生産する微生物及びｌ−アミノ酸の製造法 |
| RU2008105793A (ru) | 2008-02-19 | 2009-08-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) | Способ конструирования оперонов, содержащих трансляционно сопряженные гены, бактерия, содержащая такой оперон, способ продукции полезного метаболита и способ мониторинга экспрессии гена |
| EP2098597A1 (de) | 2008-03-04 | 2009-09-09 | Evonik Degussa GmbH | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von verbesserten Stämmen der Familie Enterobacteriaceae |
| DE102008002309A1 (de) | 2008-06-09 | 2009-12-10 | Evonik Degussa Gmbh | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von verbesserten Stämmen der Familie Enterobacteriaceae |
| DE102008040352A1 (de) | 2008-07-11 | 2010-01-14 | Evonik Degussa Gmbh | Verfahren zur Herstellung von L-Tryptophan unter Verwendung von verbesserten Stämmen der Familie Enterobacteriaceae |
| DE102008044768A1 (de) | 2008-08-28 | 2010-03-04 | Evonik Degussa Gmbh | Verfahren zur Herstellung von organisch-chemischen Verbindungen unter Verwendung von verbesserten Stämmen der Familie Enterobacteriaceae |
| EP2336347B1 (en) | 2008-09-08 | 2017-03-15 | Ajinomoto Co., Inc. | An l-amino acid-producing microorganism and a method for producing an l-amino acid |
| JP2012029565A (ja) | 2008-11-27 | 2012-02-16 | Ajinomoto Co Inc | Ｌ−アミノ酸の製造法 |
| JP2010142200A (ja) | 2008-12-22 | 2010-07-01 | Ajinomoto Co Inc | Ｌ−リジンの製造法 |
| BRPI1007069A2 (pt) | 2009-01-23 | 2015-08-25 | Ajinomoto Kk | Método para produzir um l-aminoácido. |
| EP2267145A1 (de) | 2009-06-24 | 2010-12-29 | Evonik Degussa GmbH | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von verbesserten Stämmen der Familie Enterobacteriaceae |
| JPWO2011013707A1 (ja) | 2009-07-29 | 2013-01-10 | 味の素株式会社 | Ｌ−アミノ酸の製造法 |
| JP2012223092A (ja) | 2009-08-28 | 2012-11-15 | Ajinomoto Co Inc | Ｌ−アミノ酸の製造法 |
| JP2013013329A (ja) | 2009-11-06 | 2013-01-24 | Ajinomoto Co Inc | Ｌ−アミノ酸の製造法 |
| RU2460793C2 (ru) | 2010-01-15 | 2012-09-10 | Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО АГРИ) | Способ получения l-аминокислот с использованием бактерий семейства enterobacteriaceae |
| JP2013074795A (ja) | 2010-02-08 | 2013-04-25 | Ajinomoto Co Inc | 変異型ｒｐｓＡ遺伝子及びＬ−アミノ酸の製造法 |
| RU2471868C2 (ru) | 2010-02-18 | 2013-01-10 | Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО АГРИ) | Мутантная аденилатциклаза, днк, кодирующая ее, бактерия семейства enterobacteriaceae, содержащая указанную днк, и способ получения l-аминокислот |
| RU2482188C2 (ru) | 2010-07-21 | 2013-05-20 | Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО АГРИ) | СПОСОБ ПОЛУЧЕНИЯ L-АРГИНИНА С ИСПОЛЬЗОВАНИЕМ БАКТЕРИЙ РОДА Escherichia, В КОТОРОЙ ИНАКТИВИРОВАН ОПЕРОН astCADBE |
| RU2501858C2 (ru) | 2010-07-21 | 2013-12-20 | Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО АГРИ) | СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТЫ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ СЕМЕЙСТВА Enterobacteriaceae |
| JP2014036576A (ja) | 2010-12-10 | 2014-02-27 | Ajinomoto Co Inc | Ｌ−アミノ酸の製造法 |
| RU2011134436A (ru) | 2011-08-18 | 2013-10-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") | Способ получения l-аминокислоты с использованием бактерии семейства enterobacteriaceae, обладающей повышенной экспрессией генов каскада образования флагелл и клеточной подвижности |
| EP2628792A1 (de) | 2012-02-17 | 2013-08-21 | Evonik Industries AG | Zelle mit verringerter ppGppase-Aktivität |
| EP2762571A1 (de) | 2013-01-30 | 2014-08-06 | Evonik Industries AG | Mikroorganismus und Verfahren zur fermentativen Herstellung von Aminosäuren |
| RU2013118637A (ru) | 2013-04-23 | 2014-10-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") | СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ СЕМЕЙСТВА ENTEROBACTERIACEAE, В КОТОРОЙ РАЗРЕГУЛИРОВАН ГЕН yjjK |
| PE20150681A1 (es) | 2013-05-13 | 2015-05-15 | Ajinomoto Kk | Metodo para producir l-aminoacidos |
| JP2016165225A (ja) | 2013-07-09 | 2016-09-15 | 味の素株式会社 | 有用物質の製造方法 |
| RU2013140115A (ru) | 2013-08-30 | 2015-03-10 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") | СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ СЕМЕЙСТВА Enterobacteriaceae, В КОТОРОЙ НАРУШЕНА ЭКСПРЕССИЯ КЛАСТЕРА ГЕНОВ znuACB |
| JP2016192903A (ja) | 2013-09-17 | 2016-11-17 | 味の素株式会社 | 海藻由来バイオマスからのｌ−アミノ酸の製造方法 |
| JP5958653B2 (ja) | 2013-10-02 | 2016-08-02 | 味の素株式会社 | アンモニア制御装置およびアンモニア制御方法 |
| RU2013144250A (ru) | 2013-10-02 | 2015-04-10 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") | СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ СЕМЕЙСТВА Enterobacteriaceae, В КОТОРОЙ ОСЛАБЛЕНА ЭКСПРЕССИЯ ГЕНА, КОДИРУЮЩЕГО ФОСФАТНЫЙ ТРАНСПОРТЕР |
| JP6459962B2 (ja) | 2013-10-21 | 2019-01-30 | 味の素株式会社 | Ｌ−アミノ酸の製造法 |
| JP6519476B2 (ja) | 2013-10-23 | 2019-05-29 | 味の素株式会社 | 目的物質の製造法 |
| RU2014105547A (ru) | 2014-02-14 | 2015-08-20 | Адзиномото Ко., Инк. | СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ СЕМЕЙСТВА ENTEROBACTERIACEAE, ИМЕЮЩЕЙ СВЕРХЭКСПРЕССИРУЕМЫЙ ГЕН yajL |
| RU2015120052A (ru) | 2015-05-28 | 2016-12-20 | Аджиномото Ко., Инк. | Способ получения L-аминокислоты с использованием бактерии семейства Enterobacteriaceae, в которой ослаблена экспрессия гена gshA |
| CN105316371B (zh) * | 2015-10-23 | 2018-08-14 | 安徽丰原发酵技术工程研究有限公司 | 一种用于提高色氨酸发酵产量的方法 |
| BR112018017227A2 (pt) | 2016-02-25 | 2019-02-05 | Ajinomoto Kk | método para produzir um l-aminoácido |
| RU2760290C1 (ru) * | 2016-07-08 | 2021-11-23 | Метаболик Эксплорер | Способ ферментативной продукции целевых молекул микроорганизмами, включающими гены, кодирующие фосфотрансферазную систему (фтс) сахаров |
| JP7066977B2 (ja) | 2017-04-03 | 2022-05-16 | 味の素株式会社 | Ｌ－アミノ酸の製造法 |
| EP3385275B1 (de) | 2017-04-07 | 2019-10-23 | Evonik Degussa GmbH | Verfahren zur herstellung von aromatischen l-aminosäuren unter verwendung von verbesserten stämmen der familie enterobacteriaceae |
| JP7034496B2 (ja) * | 2017-08-07 | 2022-03-14 | 地方独立行政法人大阪産業技術研究所 | 芳香族化合物を産生する微生物 |
| US11053526B2 (en) | 2018-08-09 | 2021-07-06 | Evonik Operations Gmbh | Process for preparing L amino acids using improved strains of the enterobacteriaceae family |
| EP3608409A1 (en) | 2018-08-09 | 2020-02-12 | Evonik Operations GmbH | Process for preparing l amino acids using improved strains of the enterobacteriaceae family |
| WO2020071538A1 (en) | 2018-10-05 | 2020-04-09 | Ajinomoto Co., Inc. | Method for producing target substance by bacterial fermentation |
| BR112021014194A2 (pt) | 2019-02-22 | 2021-12-28 | Ajinomoto Kk | Método para a produção de um l-aminoácido |
| BR112021017870A2 (pt) | 2019-04-05 | 2021-12-07 | Ajinomoto Kk | Método para produzir um l-aminoácido |
| JP2022550084A (ja) | 2019-09-25 | 2022-11-30 | 味の素株式会社 | 細菌の発酵によるｌ－アミノ酸の製造方法 |
| WO2022245340A1 (en) * | 2021-05-18 | 2022-11-24 | Zymergen Inc. | Engineered biosynthetic pathways for production of deoxyhydrochorismic acid by fermentation |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5236831A (en) * | 1981-12-29 | 1993-08-17 | Kiowa Hakko Kogyo Co., Ltd. | Amino acid synthesis in corynebacteria using E. coli genes |
| DE3576523D1 (de) | 1984-11-20 | 1990-04-19 | Ajinomoto Kk | Rekombinante dna enthaltende coryneformbakterien und verfahren zur herstellung von aromatischen aminosaeuren unter verwendung dieser bakterien. |
| WO1990004636A1 (en) * | 1988-10-25 | 1990-05-03 | Vsesojuzny Nauchno-Issledovatelsky Institut Genetiki I Selektsii Promyshlennykh Mikroorganizmov (Vniigenetika) | Strain of bacteria escherichia coli, producer of l-threonine |
| JPH02472A (ja) | 1988-12-26 | 1990-01-05 | Kyowa Hakko Kogyo Co Ltd | Ｌ―グルタミン酸の製造方法 |
| NZ238342A (en) * | 1990-06-04 | 1993-10-26 | Univ Notre Dame Du Lac | Preparation of beta-hydroxy-alphaamino acids useful as intermediates in the preparation of beta-lactam antibiotics |
| JP3880636B2 (ja) | 1994-01-10 | 2007-02-14 | 味の素株式会社 | 発酵法によるｌ−グルタミン酸の製造法 |
| US5998178A (en) * | 1994-05-30 | 1999-12-07 | Ajinomoto Co., Ltd. | L-isoleucine-producing bacterium and method for preparing L-isoleucine through fermentation |
| MX9702011A (es) * | 1994-09-16 | 1998-04-30 | Texas A & M Univ Sys | Mocrioorganismos y metodos para la sobreproduccion de dahp mediante el gen pps clonado. |
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- 1996-08-27 SK SK235-98A patent/SK282611B6/sk not_active IP Right Cessation
- 1996-08-27 US US09/011,762 patent/US6319696B1/en not_active Expired - Lifetime
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- 1996-08-27 WO PCT/JP1996/002399 patent/WO1997008333A1/ja active IP Right Grant
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| EP0877090A4 (en) | 2001-04-18 |
| US6319696B1 (en) | 2001-11-20 |
| PL183472B1 (pl) | 2002-06-28 |
| PL325155A1 (en) | 1998-07-06 |
| DK0877090T3 (da) | 2005-12-27 |
| HUP9901198A2 (hu) | 1999-07-28 |
| CN1077139C (zh) | 2002-01-02 |
| CN1200150A (zh) | 1998-11-25 |
| HU224895B1 (en) | 2006-04-28 |
| WO1997008333A1 (fr) | 1997-03-06 |
| SK23598A3 (en) | 1998-09-09 |
| EP0877090B1 (en) | 2005-11-23 |
| EP0877090A1 (en) | 1998-11-11 |
| JPH09121872A (ja) | 1997-05-13 |
| HUP9901198A3 (en) | 2001-10-29 |
| DE69635493T2 (de) | 2006-08-03 |
| DE69635493D1 (de) | 2005-12-29 |
| JP4032441B2 (ja) | 2008-01-16 |
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Expiry date: 20160827 |