SG189496A1 - Novel coelenterazine substrates and methods of use - Google Patents

Info

Publication number

SG189496A1

SG189496A1 SG2013030366A SG2013030366A SG189496A1 SG 189496 A1 SG189496 A1 SG 189496A1 SG 2013030366 A SG2013030366 A SG 2013030366A SG 2013030366 A SG2013030366 A SG 2013030366A SG 189496 A1 SG189496 A1 SG 189496A1

Authority

SG

Singapore

Prior art keywords

pbi

coelenterazine

luminescence

variants

variant

Prior art date

2010-11-02

Links

• Espacenet
• Global Dossier
• Discuss

• 238000000034 method Methods 0.000 title claims description 69
• 239000000758 substrate Substances 0.000 title abstract description 178
• YHIPILPTUVMWQT-UHFFFAOYSA-N Oplophorus luciferin Chemical compound C1=CC(O)=CC=C1CC(C(N1C=C(N2)C=3C=CC(O)=CC=3)=O)=NC1=C2CC1=CC=CC=C1 YHIPILPTUVMWQT-UHFFFAOYSA-N 0.000 title description 518
• 238000004020 luminiscence type Methods 0.000 claims abstract description 403
• 108060001084 Luciferase Proteins 0.000 claims abstract description 182
• 239000005089 Luciferase Substances 0.000 claims abstract description 150
• 241001443978 Oplophorus Species 0.000 claims abstract description 19
• 239000003153 chemical reaction reagent Substances 0.000 claims description 73
• 150000001875 compounds Chemical class 0.000 claims description 65
• 125000000217 alkyl group Chemical group 0.000 claims description 37
• 108010052090 Renilla Luciferases Proteins 0.000 claims description 24
• 239000000872 buffer Substances 0.000 claims description 15
• 125000000753 cycloalkyl group Chemical group 0.000 claims description 14
• 241001465754 Metazoa Species 0.000 claims description 7
• 229920006395 saturated elastomer Polymers 0.000 claims description 7
• 102100028651 Tenascin-N Human genes 0.000 claims description 2
• 101710087911 Tenascin-N Proteins 0.000 claims description 2
• 230000009261 transgenic effect Effects 0.000 claims 3
• 125000001475 halogen functional group Chemical group 0.000 claims 1
• 125000003275 alpha amino acid group Chemical group 0.000 abstract description 148
• 108090000623 proteins and genes Proteins 0.000 abstract description 146
• 238000006467 substitution reaction Methods 0.000 abstract description 137
• 102000004169 proteins and genes Human genes 0.000 abstract description 112
• 108090000765 processed proteins & peptides Proteins 0.000 abstract description 88
• 229920001184 polypeptide Polymers 0.000 abstract description 84
• 102000004196 processed proteins & peptides Human genes 0.000 abstract description 84
• 150000001413 amino acids Chemical class 0.000 abstract description 43
• 102000040430 polynucleotide Human genes 0.000 abstract description 43
• 108091033319 polynucleotide Proteins 0.000 abstract description 43
• 239000002157 polynucleotide Substances 0.000 abstract description 43
• 210000004027 cell Anatomy 0.000 description 392
• 235000001014 amino acid Nutrition 0.000 description 151
• HTBLMRUZSCCOLL-UHFFFAOYSA-N 8-benzyl-2-(furan-2-ylmethyl)-6-phenylimidazo[1,2-a]pyrazin-3-ol Chemical compound OC1=C(CC2=CC=CO2)N=C2N1C=C(N=C2CC1=CC=CC=C1)C1=CC=CC=C1 HTBLMRUZSCCOLL-UHFFFAOYSA-N 0.000 description 131
• YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 102
• 235000018102 proteins Nutrition 0.000 description 86
• 230000000694 effects Effects 0.000 description 77
• 239000006166 lysate Substances 0.000 description 77
• OHCQJHSOBUTRHG-KGGHGJDLSA-N FORSKOLIN Chemical compound O=C([C@@]12O)C[C@](C)(C=C)O[C@]1(C)[C@@H](OC(=O)C)[C@@H](O)[C@@H]1[C@]2(C)[C@@H](O)CCC1(C)C OHCQJHSOBUTRHG-KGGHGJDLSA-N 0.000 description 76
• 238000003556 assay Methods 0.000 description 73
• 108020004705 Codon Proteins 0.000 description 71
• 230000014509 gene expression Effects 0.000 description 69
• 102220565379 Factor in the germline alpha_A4E_mutation Human genes 0.000 description 64
• XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 62
• 150000007523 nucleic acids Chemical group 0.000 description 58
• 239000000523 sample Substances 0.000 description 56
• 108020004414 DNA Proteins 0.000 description 55
• 102000004190 Enzymes Human genes 0.000 description 55
• 108090000790 Enzymes Proteins 0.000 description 55
• 241000588724 Escherichia coli Species 0.000 description 55
• 229940088598 enzyme Drugs 0.000 description 55
• 238000001890 transfection Methods 0.000 description 53
• 230000002103 transcriptional effect Effects 0.000 description 52
• 230000004927 fusion Effects 0.000 description 51
• 239000000243 solution Substances 0.000 description 49
• 239000000203 mixture Substances 0.000 description 46
• 239000013598 vector Substances 0.000 description 45
• 102220615425 40S ribosomal protein S13_K43R_mutation Human genes 0.000 description 44
• 229940024606 amino acid Drugs 0.000 description 44
• UMGDCJDMYOKAJW-UHFFFAOYSA-N thiourea Chemical compound NC(N)=S UMGDCJDMYOKAJW-UHFFFAOYSA-N 0.000 description 44
• 230000001580 bacterial effect Effects 0.000 description 41
• 230000008859 change Effects 0.000 description 40
• -1 coelenterazine-hep Chemical compound 0.000 description 40
• 239000012634 fragment Substances 0.000 description 39
• SUZLHDUTVMZSEV-UHFFFAOYSA-N Deoxycoleonol Natural products C12C(=O)CC(C)(C=C)OC2(C)C(OC(=O)C)C(O)C2C1(C)C(O)CCC2(C)C SUZLHDUTVMZSEV-UHFFFAOYSA-N 0.000 description 38
• OHCQJHSOBUTRHG-UHFFFAOYSA-N colforsin Natural products OC12C(=O)CC(C)(C=C)OC1(C)C(OC(=O)C)C(O)C1C2(C)C(O)CCC1(C)C OHCQJHSOBUTRHG-UHFFFAOYSA-N 0.000 description 38
• 230000028327 secretion Effects 0.000 description 37
• 238000006243 chemical reaction Methods 0.000 description 36
• 108020001507 fusion proteins Proteins 0.000 description 35
• 108091028043 Nucleic acid sequence Proteins 0.000 description 34
• VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 34
• 102000037865 fusion proteins Human genes 0.000 description 34
• OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 33
• 230000015572 biosynthetic process Effects 0.000 description 33
• LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 32
• 108090000331 Firefly luciferases Proteins 0.000 description 31
• 230000001965 increasing effect Effects 0.000 description 31
• XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 31
• 235000019439 ethyl acetate Nutrition 0.000 description 30
• 238000003786 synthesis reaction Methods 0.000 description 30
• 230000001976 improved effect Effects 0.000 description 28
• 239000012139 lysis buffer Substances 0.000 description 27
• FVEFRICMTUKAML-UHFFFAOYSA-M sodium tetradecyl sulfate Chemical compound [Na+].CCCCC(CC)CCC(CC(C)C)OS([O-])(=O)=O FVEFRICMTUKAML-UHFFFAOYSA-M 0.000 description 27
• KAEGGIFPLJZUOZ-UHFFFAOYSA-N Renilla luciferin Chemical compound C1=CC(O)=CC=C1C(N1)=CN2C(=O)C(CC=3C=CC=CC=3)=NC2=C1CC1=CC=CC=C1 KAEGGIFPLJZUOZ-UHFFFAOYSA-N 0.000 description 26
• XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 26
• 239000011541 reaction mixture Substances 0.000 description 26
• 108010076504 Protein Sorting Signals Proteins 0.000 description 25
• 102000039446 nucleic acids Human genes 0.000 description 25
• 108020004707 nucleic acids Proteins 0.000 description 25
• IMNFDUFMRHMDMM-UHFFFAOYSA-N N-Heptane Chemical class CCCCCCC IMNFDUFMRHMDMM-UHFFFAOYSA-N 0.000 description 24
• 238000004458 analytical method Methods 0.000 description 24
• 239000002773 nucleotide Substances 0.000 description 24
• 125000003729 nucleotide group Chemical group 0.000 description 24
• 239000013612 plasmid Substances 0.000 description 24
• 239000002904 solvent Substances 0.000 description 23
• 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 22
• 108091003079 Bovine Serum Albumin Proteins 0.000 description 21
• 239000012131 assay buffer Substances 0.000 description 21
• RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 20
• WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 20
• 239000000047 product Substances 0.000 description 20
• KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical compound [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 description 19
• 230000004044 response Effects 0.000 description 19
• VASZYFIKPKYGNC-UHFFFAOYSA-N 2-[[2-[bis(carboxymethyl)amino]cyclohexyl]-(carboxymethyl)amino]acetic acid;hydrate Chemical compound O.OC(=O)CN(CC(O)=O)C1CCCCC1N(CC(O)=O)CC(O)=O VASZYFIKPKYGNC-UHFFFAOYSA-N 0.000 description 18
• IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 18
• 241000242739 Renilla Species 0.000 description 18
• 241000927894 Sergestes Species 0.000 description 18
• 102000040945 Transcription factor Human genes 0.000 description 18
• 108091023040 Transcription factor Proteins 0.000 description 18
• 239000012091 fetal bovine serum Substances 0.000 description 18
• 239000007787 solid Substances 0.000 description 18
• 230000006698 induction Effects 0.000 description 17
• 230000027455 binding Effects 0.000 description 16
• 230000006872 improvement Effects 0.000 description 16
• 230000002829 reductive effect Effects 0.000 description 16
• 230000001105 regulatory effect Effects 0.000 description 16
• DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 description 16
• QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 15
• 102000030914 Fatty Acid-Binding Human genes 0.000 description 15
• 108091022862 fatty acid binding Proteins 0.000 description 15
• 102220315726 rs138189735 Human genes 0.000 description 15
• 241000894007 species Species 0.000 description 14
• 239000000126 substance Substances 0.000 description 14
• JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 13
• 230000002101 lytic effect Effects 0.000 description 13
• 210000004962 mammalian cell Anatomy 0.000 description 13
• 238000005259 measurement Methods 0.000 description 13
• 238000012360 testing method Methods 0.000 description 13
• 239000007995 HEPES buffer Substances 0.000 description 12
• 102220493633 HLA class II histocompatibility antigen, DRB1 beta chain_S66N_mutation Human genes 0.000 description 12
• JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 12
• 108091027981 Response element Proteins 0.000 description 12
• KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 12
• 150000003839 salts Chemical class 0.000 description 12
• 101100226596 Gallus gallus FABP gene Proteins 0.000 description 11
• 241000282414 Homo sapiens Species 0.000 description 11
• 230000003833 cell viability Effects 0.000 description 11
• 230000007423 decrease Effects 0.000 description 11
• 229910052739 hydrogen Inorganic materials 0.000 description 11
• 238000011534 incubation Methods 0.000 description 11
• NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 11
• 238000004949 mass spectrometry Methods 0.000 description 11
• 239000000741 silica gel Substances 0.000 description 11
• 229910002027 silica gel Inorganic materials 0.000 description 11
• 239000012096 transfection reagent Substances 0.000 description 11
• 241000059559 Agriotes sordidus Species 0.000 description 10
• QOSSAOTZNIDXMA-UHFFFAOYSA-N Dicylcohexylcarbodiimide Chemical compound C1CCCCC1N=C=NC1CCCCC1 QOSSAOTZNIDXMA-UHFFFAOYSA-N 0.000 description 10
• AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 10
• 241000522587 Oplophorus gracilirostris Species 0.000 description 10
• 102220525955 Polycomb group RING finger protein 1_Y109F_mutation Human genes 0.000 description 10
• 241000699284 Sergia Species 0.000 description 10
• 239000012267 brine Substances 0.000 description 10
• 229910002091 carbon monoxide Inorganic materials 0.000 description 10
• 238000012217 deletion Methods 0.000 description 10
• 230000037430 deletion Effects 0.000 description 10
• 238000003780 insertion Methods 0.000 description 10
• 230000037431 insertion Effects 0.000 description 10
• 230000003993 interaction Effects 0.000 description 10
• AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 10
• 229960000310 isoleucine Drugs 0.000 description 10
• 239000003446 ligand Substances 0.000 description 10
• 239000000463 material Substances 0.000 description 10
• 239000002609 medium Substances 0.000 description 10
• HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 10
• YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 10
• 238000013518 transcription Methods 0.000 description 10
• 230000035897 transcription Effects 0.000 description 10
• 108700032225 Antioxidant Response Elements Proteins 0.000 description 9
• 108010043121 Green Fluorescent Proteins Proteins 0.000 description 9
• 241000254158 Lampyridae Species 0.000 description 9
• 210000004899 c-terminal region Anatomy 0.000 description 9
• 239000003112 inhibitor Substances 0.000 description 9
• 102220173399 rs146534475 Human genes 0.000 description 9
• 238000002741 site-directed mutagenesis Methods 0.000 description 9
• 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 9
• 210000001519 tissue Anatomy 0.000 description 9
• 231100000419 toxicity Toxicity 0.000 description 9
• 230000001988 toxicity Effects 0.000 description 9
• HNGMHWCZZWCBON-UHFFFAOYSA-N 3-benzyl-5-phenylpyrazin-2-amine Chemical compound NC1=NC=C(C=2C=CC=CC=2)N=C1CC1=CC=CC=C1 HNGMHWCZZWCBON-UHFFFAOYSA-N 0.000 description 8
• 241000695178 Acanthephyra Species 0.000 description 8
• 239000004475 Arginine Substances 0.000 description 8
• 102000004144 Green Fluorescent Proteins Human genes 0.000 description 8
• OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 8
• KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 8
• 239000004472 Lysine Substances 0.000 description 8
• FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
• 239000002253 acid Substances 0.000 description 8
• 239000012491 analyte Substances 0.000 description 8
• ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 8
• 230000003247 decreasing effect Effects 0.000 description 8
• 238000010790 dilution Methods 0.000 description 8
• 239000012895 dilution Substances 0.000 description 8
• 239000000284 extract Substances 0.000 description 8
• 238000003818 flash chromatography Methods 0.000 description 8
• 239000005090 green fluorescent protein Substances 0.000 description 8
• 239000000843 powder Substances 0.000 description 8
• 102220257007 rs1387584638 Human genes 0.000 description 8
• 229940054269 sodium pyruvate Drugs 0.000 description 8
• FIAFUQMPZJWCLV-UHFFFAOYSA-N suramin Chemical compound OS(=O)(=O)C1=CC(S(O)(=O)=O)=C2C(NC(=O)C3=CC=C(C(=C3)NC(=O)C=3C=C(NC(=O)NC=4C=C(C=CC=4)C(=O)NC=4C(=CC=C(C=4)C(=O)NC=4C5=C(C=C(C=C5C(=CC=4)S(O)(=O)=O)S(O)(=O)=O)S(O)(=O)=O)C)C=CC=3)C)=CC=C(S(O)(=O)=O)C2=C1 FIAFUQMPZJWCLV-UHFFFAOYSA-N 0.000 description 8
• 229960005314 suramin Drugs 0.000 description 8
• 102100022715 Acetyl-coenzyme A thioesterase Human genes 0.000 description 7
• IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 7
• CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 description 7
• BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 description 7
• 101000678862 Homo sapiens Acetyl-coenzyme A thioesterase Proteins 0.000 description 7
• DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 description 7
• 102000006830 Luminescent Proteins Human genes 0.000 description 7
• 108010047357 Luminescent Proteins Proteins 0.000 description 7
• BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 7
• 101100028037 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) prlC gene Proteins 0.000 description 7
• 241000251539 Vertebrata <Metazoa> Species 0.000 description 7
• 230000006907 apoptotic process Effects 0.000 description 7
• 239000013592 cell lysate Substances 0.000 description 7
• 230000000295 complement effect Effects 0.000 description 7
• 238000001514 detection method Methods 0.000 description 7
• 239000010410 layer Substances 0.000 description 7
• 235000005772 leucine Nutrition 0.000 description 7
• 238000003670 luciferase enzyme activity assay Methods 0.000 description 7
• 238000005457 optimization Methods 0.000 description 7
• 229960003531 phenolsulfonphthalein Drugs 0.000 description 7
• 238000003157 protein complementation Methods 0.000 description 7
• 238000012216 screening Methods 0.000 description 7
• BWNPYAKFDUFNND-UHFFFAOYSA-N 4-(5-amino-6-benzylpyrazin-2-yl)phenol Chemical compound NC1=NC=C(C=2C=CC(O)=CC=2)N=C1CC1=CC=CC=C1 BWNPYAKFDUFNND-UHFFFAOYSA-N 0.000 description 6
• WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 6
• 241000196324 Embryophyta Species 0.000 description 6
• DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 6
• UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 6
• 102000004889 Interleukin-6 Human genes 0.000 description 6
• 108090001005 Interleukin-6 Proteins 0.000 description 6
• WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 6
• ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 6
• ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 6
• 241000186140 Metridia longa Species 0.000 description 6
• ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 6
• UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 6
• HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
• DKGAVHZHDRPRBM-UHFFFAOYSA-N Tert-Butanol Chemical compound CC(C)(C)O DKGAVHZHDRPRBM-UHFFFAOYSA-N 0.000 description 6
• ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 6
• 238000000225 bioluminescence resonance energy transfer Methods 0.000 description 6
• 230000010261 cell growth Effects 0.000 description 6
• 238000003570 cell viability assay Methods 0.000 description 6
• 238000012512 characterization method Methods 0.000 description 6
• 238000004587 chromatography analysis Methods 0.000 description 6
• 238000001816 cooling Methods 0.000 description 6
• 238000002474 experimental method Methods 0.000 description 6
• 229930195712 glutamate Natural products 0.000 description 6
• 239000001257 hydrogen Substances 0.000 description 6
• 238000003384 imaging method Methods 0.000 description 6
• 230000004048 modification Effects 0.000 description 6
• 238000012986 modification Methods 0.000 description 6
• 230000035772 mutation Effects 0.000 description 6
• 239000012044 organic layer Substances 0.000 description 6
• UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 6
• 238000002708 random mutagenesis Methods 0.000 description 6
• 239000004576 sand Substances 0.000 description 6
• 239000007858 starting material Substances 0.000 description 6
• 230000035882 stress Effects 0.000 description 6
• 108091032973 (ribonucleotides)n+m Proteins 0.000 description 5
• 102000011727 Caspases Human genes 0.000 description 5
• 108010076667 Caspases Proteins 0.000 description 5
• 108700010070 Codon Usage Proteins 0.000 description 5
• 241000496718 Escherichia coli KRX Species 0.000 description 5
• 102000007399 Nuclear hormone receptor Human genes 0.000 description 5
• 108020005497 Nuclear hormone receptor Proteins 0.000 description 5
• PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 5
• 108700012920 TNF Proteins 0.000 description 5
• 102000018679 Tacrolimus Binding Proteins Human genes 0.000 description 5
• 108010027179 Tacrolimus Binding Proteins Proteins 0.000 description 5
• 102220604980 Transcription factor Sp2_A33K_mutation Human genes 0.000 description 5
• GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 description 5
• 239000002585 base Substances 0.000 description 5
• 230000008901 benefit Effects 0.000 description 5
• 238000005415 bioluminescence Methods 0.000 description 5
• 230000029918 bioluminescence Effects 0.000 description 5
• 229910052799 carbon Inorganic materials 0.000 description 5
• 238000012054 celltiter-glo Methods 0.000 description 5
• 125000000524 functional group Chemical group 0.000 description 5
• 239000007789 gas Substances 0.000 description 5
• 238000002523 gelfiltration Methods 0.000 description 5
• 230000002068 genetic effect Effects 0.000 description 5
• 125000005843 halogen group Chemical group 0.000 description 5
• 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 5
• 239000000178 monomer Substances 0.000 description 5
• 229910052763 palladium Inorganic materials 0.000 description 5
• 230000037361 pathway Effects 0.000 description 5
• 238000001742 protein purification Methods 0.000 description 5
• 102220058220 rs730881937 Human genes 0.000 description 5
• SUVMJBTUFCVSAD-UHFFFAOYSA-N sulforaphane Chemical compound CS(=O)CCCCN=C=S SUVMJBTUFCVSAD-UHFFFAOYSA-N 0.000 description 5
• 229960005559 sulforaphane Drugs 0.000 description 5
• AUIMIXCSYLLZMD-UHFFFAOYSA-N 8-benzyl-2-methyl-6-phenyl-7h-imidazo[1,2-a]pyrazin-3-one Chemical compound N1C(C=2C=CC=CC=2)=CN2C(=O)C(C)=NC2=C1CC1=CC=CC=C1 AUIMIXCSYLLZMD-UHFFFAOYSA-N 0.000 description 4
• OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
• CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
• 229920000858 Cyclodextrin Polymers 0.000 description 4
• SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 4
• 101000588302 Homo sapiens Nuclear factor erythroid 2-related factor 2 Proteins 0.000 description 4
• SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 description 4
• PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 4
• KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 4
• 241000124008 Mammalia Species 0.000 description 4
• 102100031701 Nuclear factor erythroid 2-related factor 2 Human genes 0.000 description 4
• 102000035195 Peptidases Human genes 0.000 description 4
• 108091005804 Peptidases Proteins 0.000 description 4
• 102220553089 Proenkephalin-A_F77Y_mutation Human genes 0.000 description 4
• 239000004365 Protease Substances 0.000 description 4
• KYQCOXFCLRTKLS-UHFFFAOYSA-N Pyrazine Chemical compound C1=CN=CC=N1 KYQCOXFCLRTKLS-UHFFFAOYSA-N 0.000 description 4
• LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 description 4
• 102220530818 Sclerostin_V21L_mutation Human genes 0.000 description 4
• 102000004142 Trypsin Human genes 0.000 description 4
• 108090000631 Trypsin Proteins 0.000 description 4
• KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 4
• 229960000583 acetic acid Drugs 0.000 description 4
• 150000007513 acids Chemical class 0.000 description 4
• 238000013459 approach Methods 0.000 description 4
• 239000011575 calcium Substances 0.000 description 4
• 235000011089 carbon dioxide Nutrition 0.000 description 4
• 239000006285 cell suspension Substances 0.000 description 4
• 230000001413 cellular effect Effects 0.000 description 4
• 210000000805 cytoplasm Anatomy 0.000 description 4
• 229960004132 diethyl ether Drugs 0.000 description 4
• 239000003623 enhancer Substances 0.000 description 4
• 210000003527 eukaryotic cell Anatomy 0.000 description 4
• 239000000706 filtrate Substances 0.000 description 4
• 238000002825 functional assay Methods 0.000 description 4
• 238000001641 gel filtration chromatography Methods 0.000 description 4
• 238000003365 immunocytochemistry Methods 0.000 description 4
• 230000005764 inhibitory process Effects 0.000 description 4
• 230000000155 isotopic effect Effects 0.000 description 4
• 238000004519 manufacturing process Methods 0.000 description 4
• 239000012528 membrane Substances 0.000 description 4
• 108020004999 messenger RNA Proteins 0.000 description 4
• 108020004017 nuclear receptors Proteins 0.000 description 4
• 229910052760 oxygen Inorganic materials 0.000 description 4
• 230000004481 post-translational protein modification Effects 0.000 description 4
• 230000008569 process Effects 0.000 description 4
• 210000001236 prokaryotic cell Anatomy 0.000 description 4
• 238000000746 purification Methods 0.000 description 4
• RXWNCPJZOCPEPQ-NVWDDTSBSA-N puromycin Chemical compound C1=CC(OC)=CC=C1C[C@H](N)C(=O)N[C@H]1[C@@H](O)[C@H](N2C3=NC=NC(=C3N=C2)N(C)C)O[C@@H]1CO RXWNCPJZOCPEPQ-NVWDDTSBSA-N 0.000 description 4
• ZAHRKKWIAAJSAO-UHFFFAOYSA-N rapamycin Natural products COCC(O)C(=C/C(C)C(=O)CC(OC(=O)C1CCCCN1C(=O)C(=O)C2(O)OC(CC(OC)C(=CC=CC=CC(C)CC(C)C(=O)C)C)CCC2C)C(C)CC3CCC(O)C(C3)OC)C ZAHRKKWIAAJSAO-UHFFFAOYSA-N 0.000 description 4
• 108091008146 restriction endonucleases Proteins 0.000 description 4
• 230000002441 reversible effect Effects 0.000 description 4
• 229960002930 sirolimus Drugs 0.000 description 4
• QFJCIRLUMZQUOT-HPLJOQBZSA-N sirolimus Chemical compound C1C[C@@H](O)[C@H](OC)C[C@@H]1C[C@@H](C)[C@H]1OC(=O)[C@@H]2CCCCN2C(=O)C(=O)[C@](O)(O2)[C@H](C)CC[C@H]2C[C@H](OC)/C(C)=C/C=C/C=C/[C@@H](C)C[C@@H](C)C(=O)[C@H](OC)[C@H](O)/C(C)=C/[C@@H](C)C(=O)C1 QFJCIRLUMZQUOT-HPLJOQBZSA-N 0.000 description 4
• 239000011734 sodium Substances 0.000 description 4
• 239000011780 sodium chloride Substances 0.000 description 4
• 239000012453 solvate Substances 0.000 description 4
• 230000003595 spectral effect Effects 0.000 description 4
• 239000000725 suspension Substances 0.000 description 4
• 125000000999 tert-butyl group Chemical group [H]C([H])([H])C(*)(C([H])([H])[H])C([H])([H])[H] 0.000 description 4
• 239000012588 trypsin Substances 0.000 description 4
• 239000004474 valine Substances 0.000 description 4
• WYJIHKGNYDLFGB-UHFFFAOYSA-N 3-(furan-2-yl)-2-oxopropanoic acid Chemical compound OC(=O)C(=O)CC1=CC=CO1 WYJIHKGNYDLFGB-UHFFFAOYSA-N 0.000 description 3
• KKADPXVIOXHVKN-UHFFFAOYSA-N 4-hydroxyphenylpyruvic acid Chemical compound OC(=O)C(=O)CC1=CC=C(O)C=C1 KKADPXVIOXHVKN-UHFFFAOYSA-N 0.000 description 3
• YZDCFXJUUHQUAH-UHFFFAOYSA-N 8-benzyl-2-[(4-hydroxyphenyl)methyl]-6-phenyl-7h-imidazo[1,2-a]pyrazin-3-one Chemical compound C1=CC(O)=CC=C1CC(C(N1C=C(N2)C=3C=CC=CC=3)=O)=NC1=C2CC1=CC=CC=C1 YZDCFXJUUHQUAH-UHFFFAOYSA-N 0.000 description 3
• WFDIJRYMOXRFFG-UHFFFAOYSA-N Acetic anhydride Chemical compound CC(=O)OC(C)=O WFDIJRYMOXRFFG-UHFFFAOYSA-N 0.000 description 3
• CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
• 108091026890 Coding region Proteins 0.000 description 3
• 108030000598 Dinoflagellate luciferases Proteins 0.000 description 3
• KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
• 102000020897 Formins Human genes 0.000 description 3
• 108091022623 Formins Proteins 0.000 description 3
• WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
• PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
• 239000004471 Glycine Substances 0.000 description 3
• VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 3
• COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 3
• 239000006137 Luria-Bertani broth Substances 0.000 description 3
• 108010014251 Muramidase Proteins 0.000 description 3
• 102000016943 Muramidase Human genes 0.000 description 3
• 102000036675 Myoglobin Human genes 0.000 description 3
• 108010062374 Myoglobin Proteins 0.000 description 3
• 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 3
• 241000283973 Oryctolagus cuniculus Species 0.000 description 3
• 108010058846 Ovalbumin Proteins 0.000 description 3
• 241000242583 Scyphozoa Species 0.000 description 3
• 241000607479 Yersinia pestis Species 0.000 description 3
• 150000001299 aldehydes Chemical class 0.000 description 3
• 125000005907 alkyl ester group Chemical group 0.000 description 3
• 230000004075 alteration Effects 0.000 description 3
• 150000001408 amides Chemical class 0.000 description 3
• 239000007864 aqueous solution Substances 0.000 description 3
• 125000001797 benzyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])* 0.000 description 3
• 238000011088 calibration curve Methods 0.000 description 3
• 239000011203 carbon fibre reinforced carbon Substances 0.000 description 3
• 230000003197 catalytic effect Effects 0.000 description 3
• 150000001768 cations Chemical class 0.000 description 3
• 210000004671 cell-free system Anatomy 0.000 description 3
• 210000004978 chinese hamster ovary cell Anatomy 0.000 description 3
• 229910052801 chlorine Inorganic materials 0.000 description 3
• 238000004440 column chromatography Methods 0.000 description 3
• 239000003398 denaturant Substances 0.000 description 3
• 238000009826 distribution Methods 0.000 description 3
• 238000010828 elution Methods 0.000 description 3
• 238000011156 evaluation Methods 0.000 description 3
• 239000012894 fetal calf serum Substances 0.000 description 3
• 238000001914 filtration Methods 0.000 description 3
• 238000001943 fluorescence-activated cell sorting Methods 0.000 description 3
• 102000034287 fluorescent proteins Human genes 0.000 description 3
• 108091006047 fluorescent proteins Proteins 0.000 description 3
• 230000005714 functional activity Effects 0.000 description 3
• 239000008103 glucose Substances 0.000 description 3
• 230000012010 growth Effects 0.000 description 3
• 239000001963 growth medium Substances 0.000 description 3
• 238000004128 high performance liquid chromatography Methods 0.000 description 3
• WJRBRSLFGCUECM-UHFFFAOYSA-N hydantoin Chemical compound O=C1CNC(=O)N1 WJRBRSLFGCUECM-UHFFFAOYSA-N 0.000 description 3
• IXCSERBJSXMMFS-UHFFFAOYSA-N hydrogen chloride Substances Cl.Cl IXCSERBJSXMMFS-UHFFFAOYSA-N 0.000 description 3
• 229910000041 hydrogen chloride Inorganic materials 0.000 description 3
• 125000001165 hydrophobic group Chemical group 0.000 description 3
• 210000003000 inclusion body Anatomy 0.000 description 3
• 230000010354 integration Effects 0.000 description 3
• 239000004325 lysozyme Substances 0.000 description 3
• 229960000274 lysozyme Drugs 0.000 description 3
• 235000010335 lysozyme Nutrition 0.000 description 3
• 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 description 3
• 239000006151 minimal media Substances 0.000 description 3
• 125000004123 n-propyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])* 0.000 description 3
• 239000013642 negative control Substances 0.000 description 3
• 229940092253 ovalbumin Drugs 0.000 description 3
• 230000003647 oxidation Effects 0.000 description 3
• 238000007254 oxidation reaction Methods 0.000 description 3
• 230000036542 oxidative stress Effects 0.000 description 3
• KXFJZKUFXHWWAJ-UHFFFAOYSA-N p-hydroxybenzoylformic acid Natural products OC(=O)C(=O)C1=CC=C(O)C=C1 KXFJZKUFXHWWAJ-UHFFFAOYSA-N 0.000 description 3
• 239000012466 permeate Substances 0.000 description 3
• COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 3
• 238000007747 plating Methods 0.000 description 3
• NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 3
• 238000012545 processing Methods 0.000 description 3
• 125000006239 protecting group Chemical group 0.000 description 3
• 230000006916 protein interaction Effects 0.000 description 3
• 102200010841 rs1553925558 Human genes 0.000 description 3
• 102220245301 rs1555596673 Human genes 0.000 description 3
• 238000013207 serial dilution Methods 0.000 description 3
• 229910052938 sodium sulfate Inorganic materials 0.000 description 3
• 235000011152 sodium sulphate Nutrition 0.000 description 3
• 238000003756 stirring Methods 0.000 description 3
• 230000009221 stress response pathway Effects 0.000 description 3
• 229910052717 sulfur Inorganic materials 0.000 description 3
• 230000005030 transcription termination Effects 0.000 description 3
• 238000012546 transfer Methods 0.000 description 3
• LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
• 230000003612 virological effect Effects 0.000 description 3
• 239000003039 volatile agent Substances 0.000 description 3
• OZFAFGSSMRRTDW-UHFFFAOYSA-N (2,4-dichlorophenyl) benzenesulfonate Chemical compound ClC1=CC(Cl)=CC=C1OS(=O)(=O)C1=CC=CC=C1 OZFAFGSSMRRTDW-UHFFFAOYSA-N 0.000 description 2
• MLNKXLRYCLKJSS-RMKNXTFCSA-N (2e)-2-hydroxyimino-1-phenylethanone Chemical compound O\N=C\C(=O)C1=CC=CC=C1 MLNKXLRYCLKJSS-RMKNXTFCSA-N 0.000 description 2
• UMGQVUWXNOJOSJ-DGGAMASNSA-N (e)-2-cyano-3-(3,4-dihydroxyphenyl)-n-[(1s)-1-phenylethyl]prop-2-enamide Chemical compound N([C@@H](C)C=1C=CC=CC=1)C(=O)C(\C#N)=C\C1=CC=C(O)C(O)=C1 UMGQVUWXNOJOSJ-DGGAMASNSA-N 0.000 description 2
• HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 2
• HNYQNKMOZDXZKZ-UHFFFAOYSA-N 2-[(3-benzyl-5-phenylpyrazin-2-yl)amino]butanoic acid Chemical compound CCC(C(O)=O)NC1=NC=C(C=2C=CC=CC=2)N=C1CC1=CC=CC=C1 HNYQNKMOZDXZKZ-UHFFFAOYSA-N 0.000 description 2
• YPZDVAOPDZMSDP-UHFFFAOYSA-N 2-[[3-benzyl-5-(4-hydroxyphenyl)pyrazin-2-yl]amino]hexanoic acid Chemical compound CCCCC(C(O)=O)NC1=NC=C(C=2C=CC(O)=CC=2)N=C1CC1=CC=CC=C1 YPZDVAOPDZMSDP-UHFFFAOYSA-N 0.000 description 2
• PUJJHVLHTWSRPM-UHFFFAOYSA-N 2-[[3-benzyl-5-(4-hydroxyphenyl)pyrazin-2-yl]amino]pentanoic acid Chemical compound CCCC(C(O)=O)NC1=NC=C(C=2C=CC(O)=CC=2)N=C1CC1=CC=CC=C1 PUJJHVLHTWSRPM-UHFFFAOYSA-N 0.000 description 2
• ZMLFFOLHKSAFBV-UHFFFAOYSA-N 2-amino-3-phenylpropanenitrile;hydrochloride Chemical compound Cl.N#CC(N)CC1=CC=CC=C1 ZMLFFOLHKSAFBV-UHFFFAOYSA-N 0.000 description 2
• DFEKJNVSDUKUIR-UHFFFAOYSA-N 2-oxo-3-thiophen-2-ylpropanoic acid Chemical compound OC(=O)C(=O)CC1=CC=CS1 DFEKJNVSDUKUIR-UHFFFAOYSA-N 0.000 description 2
• 125000003903 2-propenyl group Chemical group [H]C([*])([H])C([H])=C([H])[H] 0.000 description 2
• 125000001494 2-propynyl group Chemical group [H]C#CC([H])([H])* 0.000 description 2
• QEYYZUYXOGLKSR-UHFFFAOYSA-N 5,5,5-trifluoro-2-oxopentanoic acid Chemical compound OC(=O)C(=O)CCC(F)(F)F QEYYZUYXOGLKSR-UHFFFAOYSA-N 0.000 description 2
• ULFADACSTWUQNG-UHFFFAOYSA-N 8-benzyl-6-(4-hydroxyphenyl)-2-propyl-7h-imidazo[1,2-a]pyrazin-3-one Chemical compound N1C(C=2C=CC(O)=CC=2)=CN2C(=O)C(CCC)=NC2=C1CC1=CC=CC=C1 ULFADACSTWUQNG-UHFFFAOYSA-N 0.000 description 2
• 241001328830 Acanthephyra acanthitelsonis Species 0.000 description 2
• 241001344610 Acanthephyra acutifrons Species 0.000 description 2
• 241001655224 Acanthephyra brevirostris Species 0.000 description 2
• 241001344616 Acanthephyra cucullata Species 0.000 description 2
• 241001254943 Acanthephyra curtirostris Species 0.000 description 2
• 241000522565 Acanthephyra eximia Species 0.000 description 2
• 241001344614 Acanthephyra media Species 0.000 description 2
• 241000695171 Acanthephyra pelagica Species 0.000 description 2
• 241001254712 Acanthephyra purpurea Species 0.000 description 2
• 241000975924 Acanthephyra sanguinea Species 0.000 description 2
• 241001655223 Acanthephyra stylorostratis Species 0.000 description 2
• VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 2
• XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 2
• 241000158573 Aristeidae Species 0.000 description 2
• 241001243567 Atlanticus Species 0.000 description 2
• 241000283690 Bos taurus Species 0.000 description 2
• OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
• 102000003846 Carbonic anhydrases Human genes 0.000 description 2
• 108090000209 Carbonic anhydrases Proteins 0.000 description 2
• 108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 description 2
• 108020004635 Complementary DNA Proteins 0.000 description 2
• 102000002004 Cytochrome P-450 Enzyme System Human genes 0.000 description 2
• 108010015742 Cytochrome P-450 Enzyme System Proteins 0.000 description 2
• 230000005778 DNA damage Effects 0.000 description 2
• 231100000277 DNA damage Toxicity 0.000 description 2
• 102000016911 Deoxyribonucleases Human genes 0.000 description 2
• 108010053770 Deoxyribonucleases Proteins 0.000 description 2
• 239000012591 Dulbecco’s Phosphate Buffered Saline Substances 0.000 description 2
• 241000695271 Ephyrina Species 0.000 description 2
• 241001344608 Ephyrina bifida Species 0.000 description 2
• 241000695177 Ephyrina figueirai Species 0.000 description 2
• 241001344599 Ephyrina ombango Species 0.000 description 2
• YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 2
• 101100275612 Escherichia phage 186 CP84 gene Proteins 0.000 description 2
• QUSNBJAOOMFDIB-UHFFFAOYSA-N Ethylamine Chemical compound CCN QUSNBJAOOMFDIB-UHFFFAOYSA-N 0.000 description 2
• 102100026745 Fatty acid-binding protein, liver Human genes 0.000 description 2
• 241000233866 Fungi Species 0.000 description 2
• 102220566486 GDNF family receptor alpha-1_Q69H_mutation Human genes 0.000 description 2
• 241001328808 Gardinerosergia splendens Species 0.000 description 2
• 241000695277 Glyphus marsupialis Species 0.000 description 2
• 241001122740 Heterocarpus Species 0.000 description 2
• 241001655217 Heterogenys microphthalma Species 0.000 description 2
• 241001662190 Hymenodora glacialis Species 0.000 description 2
• 241001344603 Hymenodora gracilis Species 0.000 description 2
• 241000399688 Hymenopenaeus debilis Species 0.000 description 2
• 206010061218 Inflammation Diseases 0.000 description 2
• ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 2
• GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 2
• 241001254577 Leptochela bermudensis Species 0.000 description 2
• 241000408521 Lucida Species 0.000 description 2
• 241001655221 Lucifer typus Species 0.000 description 2
• 241000158577 Luciferidae Species 0.000 description 2
• CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
• 241001254366 Meningodora Species 0.000 description 2
• 241001344602 Meningodora mollis Species 0.000 description 2
• 241001344601 Meningodora vesca Species 0.000 description 2
• 241001321746 Mesopenaeus tropicalis Species 0.000 description 2
• 241000186243 Metridia Species 0.000 description 2
• 102000010909 Monoamine Oxidase Human genes 0.000 description 2
• 108010062431 Monoamine oxidase Proteins 0.000 description 2
• JGFZNNIVVJXRND-UHFFFAOYSA-N N,N-Diisopropylethylamine (DIPEA) Chemical compound CCN(C(C)C)C(C)C JGFZNNIVVJXRND-UHFFFAOYSA-N 0.000 description 2
• MZRVEZGGRBJDDB-UHFFFAOYSA-N N-Butyllithium Chemical compound [Li]CCCC MZRVEZGGRBJDDB-UHFFFAOYSA-N 0.000 description 2
• 229930193140 Neomycin Natural products 0.000 description 2
• 241000927863 Notostomus Species 0.000 description 2
• 241000522584 Notostomus gibbosus Species 0.000 description 2
• 108700026244 Open Reading Frames Proteins 0.000 description 2
• 241001443980 Oplophoridae Species 0.000 description 2
• 241000399690 Oplophorus spinosus Species 0.000 description 2
• 241000522601 Oplophorus typus Species 0.000 description 2
• 241001254567 Parapandalus richardi Species 0.000 description 2
• 241000695262 Parapasiphaea sulcatifrons Species 0.000 description 2
• 241000606012 Pectinatus Species 0.000 description 2
• PCNDJXKNXGMECE-UHFFFAOYSA-N Phenazine Natural products C1=CC=CC2=NC3=CC=CC=C3N=C21 PCNDJXKNXGMECE-UHFFFAOYSA-N 0.000 description 2
• 241000254064 Photinus pyralis Species 0.000 description 2
• GLUUGHFHXGJENI-UHFFFAOYSA-N Piperazine Chemical compound C1CNCCN1 GLUUGHFHXGJENI-UHFFFAOYSA-N 0.000 description 2
• NQRYJNQNLNOLGT-UHFFFAOYSA-N Piperidine Chemical compound C1CCNCC1 NQRYJNQNLNOLGT-UHFFFAOYSA-N 0.000 description 2
• 241001585061 Plesiopenaeus Species 0.000 description 2
• 239000004793 Polystyrene Substances 0.000 description 2
• 102220634786 Potassium voltage-gated channel subfamily A member 3_F77W_mutation Human genes 0.000 description 2
• LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 2
• 108020005067 RNA Splice Sites Proteins 0.000 description 2
• 241001328799 Robustosergia robusta Species 0.000 description 2
• 241000614535 Sergestes arcticus Species 0.000 description 2
• 241000927903 Sergestes similis Species 0.000 description 2
• 241001675475 Sergia lucens Species 0.000 description 2
• DWAQJAXMDSEUJJ-UHFFFAOYSA-M Sodium bisulfite Chemical compound [Na+].OS([O-])=O DWAQJAXMDSEUJJ-UHFFFAOYSA-M 0.000 description 2
• 241001660594 Solenoceridae Species 0.000 description 2
• 239000012505 Superdex™ Substances 0.000 description 2
• 241000927917 Systellaspis braueri Species 0.000 description 2
• 241000161899 Systellaspis cristata Species 0.000 description 2
• 241001254695 Systellaspis debilis Species 0.000 description 2
• 241000522580 Systellaspis pellucida Species 0.000 description 2
• 241001254524 Thalassocaris Species 0.000 description 2
• 241001254525 Thalassocaris crinita Species 0.000 description 2
• 102100037357 Thymidylate kinase Human genes 0.000 description 2
• 108700019146 Transgenes Proteins 0.000 description 2
• 239000007983 Tris buffer Substances 0.000 description 2
• 241000209140 Triticum Species 0.000 description 2
• 235000021307 Triticum Nutrition 0.000 description 2
• 230000002378 acidificating effect Effects 0.000 description 2
• 239000000908 ammonium hydroxide Substances 0.000 description 2
• 125000000129 anionic group Chemical group 0.000 description 2
• 150000001450 anions Chemical class 0.000 description 2
• 239000003443 antiviral agent Substances 0.000 description 2
• 239000012300 argon atmosphere Substances 0.000 description 2
• 125000004429 atom Chemical group 0.000 description 2
• 230000009286 beneficial effect Effects 0.000 description 2
• WGQKYBSKWIADBV-UHFFFAOYSA-N benzylamine Chemical compound NCC1=CC=CC=C1 WGQKYBSKWIADBV-UHFFFAOYSA-N 0.000 description 2
• LUFPJJNWMYZRQE-UHFFFAOYSA-N benzylsulfanylmethylbenzene Chemical compound C=1C=CC=CC=1CSCC1=CC=CC=C1 LUFPJJNWMYZRQE-UHFFFAOYSA-N 0.000 description 2
• WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
• 230000033228 biological regulation Effects 0.000 description 2
• HQABUPZFAYXKJW-UHFFFAOYSA-N butan-1-amine Chemical compound CCCCN HQABUPZFAYXKJW-UHFFFAOYSA-N 0.000 description 2
• 229910052791 calcium Inorganic materials 0.000 description 2
• 239000004202 carbamide Substances 0.000 description 2
• 125000002091 cationic group Chemical group 0.000 description 2
• 230000006037 cell lysis Effects 0.000 description 2
• KXZJHVJKXJLBKO-UHFFFAOYSA-N chembl1408157 Chemical compound N=1C2=CC=CC=C2C(C(=O)O)=CC=1C1=CC=C(O)C=C1 KXZJHVJKXJLBKO-UHFFFAOYSA-N 0.000 description 2
• 238000007385 chemical modification Methods 0.000 description 2
• 238000010367 cloning Methods 0.000 description 2
• 229940126214 compound 3 Drugs 0.000 description 2
• 108091036078 conserved sequence Proteins 0.000 description 2
• 239000012043 crude product Substances 0.000 description 2
• 230000009089 cytolysis Effects 0.000 description 2
• 108010000742 dTMP kinase Proteins 0.000 description 2
• 230000001419 dependent effect Effects 0.000 description 2
• 239000003599 detergent Substances 0.000 description 2
• 231100000673 dose–response relationship Toxicity 0.000 description 2
• 230000036267 drug metabolism Effects 0.000 description 2
• 238000005516 engineering process Methods 0.000 description 2
• 230000002255 enzymatic effect Effects 0.000 description 2
• 239000002532 enzyme inhibitor Substances 0.000 description 2
• 150000002148 esters Chemical class 0.000 description 2
• 239000012065 filter cake Substances 0.000 description 2
• 125000002485 formyl group Chemical group [H]C(*)=O 0.000 description 2
• 239000000499 gel Substances 0.000 description 2
• 239000012362 glacial acetic acid Substances 0.000 description 2
• ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 2
• LEQAOMBKQFMDFZ-UHFFFAOYSA-N glyoxal Chemical compound O=CC=O LEQAOMBKQFMDFZ-UHFFFAOYSA-N 0.000 description 2
• PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
• 229910052737 gold Inorganic materials 0.000 description 2
• 239000010931 gold Substances 0.000 description 2
• 210000005260 human cell Anatomy 0.000 description 2
• 150000002430 hydrocarbons Chemical class 0.000 description 2
• 125000004435 hydrogen atom Chemical group [H]* 0.000 description 2
• MPWOBEOETVOESI-UHFFFAOYSA-N imidazo[4,5-b]pyrazin-2-one Chemical compound N1=CC=NC2=NC(=O)N=C21 MPWOBEOETVOESI-UHFFFAOYSA-N 0.000 description 2
• 230000004054 inflammatory process Effects 0.000 description 2
• 230000037041 intracellular level Effects 0.000 description 2
• 125000000959 isobutyl group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])* 0.000 description 2
• 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 2
• 125000000468 ketone group Chemical group 0.000 description 2
• 125000001909 leucine group Chemical group [H]N(*)C(C(*)=O)C([H])([H])C(C([H])([H])[H])C([H])([H])[H] 0.000 description 2
• 239000007788 liquid Substances 0.000 description 2
• 239000011777 magnesium Substances 0.000 description 2
• 239000003550 marker Substances 0.000 description 2
• 229930182817 methionine Natural products 0.000 description 2
• 125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 description 2
• MGJXBDMLVWIYOQ-UHFFFAOYSA-N methylazanide Chemical compound [NH-]C MGJXBDMLVWIYOQ-UHFFFAOYSA-N 0.000 description 2
• 238000002156 mixing Methods 0.000 description 2
• 238000012544 monitoring process Methods 0.000 description 2
• 230000000869 mutational effect Effects 0.000 description 2
• VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical class CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 2
• 229960004927 neomycin Drugs 0.000 description 2
• 229910052757 nitrogen Inorganic materials 0.000 description 2
• 210000004940 nucleus Anatomy 0.000 description 2
• 239000003960 organic solvent Substances 0.000 description 2
• CJIIERPDFZUYPI-UHFFFAOYSA-N oxidized Oplophorus luciferin Chemical compound C1=CC(O)=CC=C1CC(=O)NC1=NC=C(C=2C=CC(O)=CC=2)N=C1CC1=CC=CC=C1 CJIIERPDFZUYPI-UHFFFAOYSA-N 0.000 description 2
• 239000002245 particle Substances 0.000 description 2
• 239000012071 phase Substances 0.000 description 2
• DTUQWGWMVIHBKE-UHFFFAOYSA-N phenylacetaldehyde Chemical compound O=CCC1=CC=CC=C1 DTUQWGWMVIHBKE-UHFFFAOYSA-N 0.000 description 2
• 239000004033 plastic Substances 0.000 description 2
• 229920003023 plastic Polymers 0.000 description 2
• 230000008488 polyadenylation Effects 0.000 description 2
• 229920002223 polystyrene Polymers 0.000 description 2
• BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical compound [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 2
• LPNYRYFBWFDTMA-UHFFFAOYSA-N potassium tert-butoxide Chemical compound [K+].CC(C)(C)[O-] LPNYRYFBWFDTMA-UHFFFAOYSA-N 0.000 description 2
• 238000002360 preparation method Methods 0.000 description 2
• 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
• 230000017854 proteolysis Effects 0.000 description 2
• 229950010131 puromycin Drugs 0.000 description 2
• XFTQRUTUGRCSGO-UHFFFAOYSA-N pyrazin-2-amine Chemical compound NC1=CN=CC=N1 XFTQRUTUGRCSGO-UHFFFAOYSA-N 0.000 description 2
• 229940076788 pyruvate Drugs 0.000 description 2
• 229940107700 pyruvic acid Drugs 0.000 description 2
• 238000010791 quenching Methods 0.000 description 2
• 230000009257 reactivity Effects 0.000 description 2
• 238000011084 recovery Methods 0.000 description 2
• 108010054624 red fluorescent protein Proteins 0.000 description 2
• 238000010992 reflux Methods 0.000 description 2
• 230000003938 response to stress Effects 0.000 description 2
• 210000001995 reticulocyte Anatomy 0.000 description 2
• 102200142166 rs35258119 Human genes 0.000 description 2
• 102200058147 rs63750353 Human genes 0.000 description 2
• 102220061993 rs786202533 Human genes 0.000 description 2
• 102200137195 rs7897309 Human genes 0.000 description 2
• 102220106669 rs879255364 Human genes 0.000 description 2
• 239000012146 running buffer Substances 0.000 description 2
• 238000009738 saturating Methods 0.000 description 2
• 125000002914 sec-butyl group Chemical group [H]C([H])([H])C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 2
• 238000000926 separation method Methods 0.000 description 2
• 230000035939 shock Effects 0.000 description 2
• 230000019491 signal transduction Effects 0.000 description 2
• 239000000377 silicon dioxide Substances 0.000 description 2
• 239000007974 sodium acetate buffer Substances 0.000 description 2
• 229910000033 sodium borohydride Inorganic materials 0.000 description 2
• 239000012279 sodium borohydride Substances 0.000 description 2
• 235000010267 sodium hydrogen sulphite Nutrition 0.000 description 2
• 210000000130 stem cell Anatomy 0.000 description 2
• 230000004936 stimulating effect Effects 0.000 description 2
• 238000000967 suction filtration Methods 0.000 description 2
• 229940124598 therapeutic candidate Drugs 0.000 description 2
• 239000010936 titanium Substances 0.000 description 2
• 231100000331 toxic Toxicity 0.000 description 2
• 230000002588 toxic effect Effects 0.000 description 2
• 230000005945 translocation Effects 0.000 description 2
• 230000032258 transport Effects 0.000 description 2
• OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 2
• 125000004417 unsaturated alkyl group Chemical group 0.000 description 2
• 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 2
• 238000001262 western blot Methods 0.000 description 2
• WQZGKKKJIJFFOK-SVZMEOIVSA-N (+)-Galactose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-SVZMEOIVSA-N 0.000 description 1
• MIOPJNTWMNEORI-GMSGAONNSA-N (S)-camphorsulfonic acid Chemical compound C1C[C@@]2(CS(O)(=O)=O)C(=O)C[C@@H]1C2(C)C MIOPJNTWMNEORI-GMSGAONNSA-N 0.000 description 1
• GETTZEONDQJALK-UHFFFAOYSA-N (trifluoromethyl)benzene Chemical compound FC(F)(F)C1=CC=CC=C1 GETTZEONDQJALK-UHFFFAOYSA-N 0.000 description 1
• ULVSHNOGEVXRDR-UHFFFAOYSA-N 1,1-dimethoxypropan-2-one Chemical compound COC(OC)C(C)=O ULVSHNOGEVXRDR-UHFFFAOYSA-N 0.000 description 1
• TUSDEZXZIZRFGC-UHFFFAOYSA-N 1-O-galloyl-3,6-(R)-HHDP-beta-D-glucose Natural products OC1C(O2)COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC1C(O)C2OC(=O)C1=CC(O)=C(O)C(O)=C1 TUSDEZXZIZRFGC-UHFFFAOYSA-N 0.000 description 1
• OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
• VGIRNWJSIRVFRT-UHFFFAOYSA-N 2',7'-difluorofluorescein Chemical compound OC(=O)C1=CC=CC=C1C1=C2C=C(F)C(=O)C=C2OC2=CC(O)=C(F)C=C21 VGIRNWJSIRVFRT-UHFFFAOYSA-N 0.000 description 1
• IHPYMWDTONKSCO-UHFFFAOYSA-N 2,2'-piperazine-1,4-diylbisethanesulfonic acid Chemical compound OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1 IHPYMWDTONKSCO-UHFFFAOYSA-N 0.000 description 1
• JNGRENQDBKMCCR-UHFFFAOYSA-N 2-(3-amino-6-iminoxanthen-9-yl)benzoic acid;hydrochloride Chemical compound [Cl-].C=12C=CC(=[NH2+])C=C2OC2=CC(N)=CC=C2C=1C1=CC=CC=C1C(O)=O JNGRENQDBKMCCR-UHFFFAOYSA-N 0.000 description 1
• 125000004182 2-chlorophenyl group Chemical group [H]C1=C([H])C(Cl)=C(*)C([H])=C1[H] 0.000 description 1
• LXBGSDVWAMZHDD-UHFFFAOYSA-N 2-methyl-1h-imidazole Chemical class CC1=NC=CN1 LXBGSDVWAMZHDD-UHFFFAOYSA-N 0.000 description 1
• TYEYBOSBBBHJIV-UHFFFAOYSA-N 2-oxobutanoic acid Chemical compound CCC(=O)C(O)=O TYEYBOSBBBHJIV-UHFFFAOYSA-N 0.000 description 1
• KDVFRMMRZOCFLS-UHFFFAOYSA-N 2-oxopentanoic acid Chemical compound CCCC(=O)C(O)=O KDVFRMMRZOCFLS-UHFFFAOYSA-N 0.000 description 1
• 101710091601 21 kDa protein Proteins 0.000 description 1
• ZOOGRGPOEVQQDX-UUOKFMHZSA-N 3',5'-cyclic GMP Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=C(NC2=O)N)=C2N=C1 ZOOGRGPOEVQQDX-UUOKFMHZSA-N 0.000 description 1
• 125000004179 3-chlorophenyl group Chemical group [H]C1=C([H])C(*)=C([H])C(Cl)=C1[H] 0.000 description 1
• OMEBKMLPDOPNNC-UHFFFAOYSA-N 3-cyclopropyl-5-phenylpyrazin-2-amine Chemical compound NC1=NC=C(C=2C=CC=CC=2)N=C1C1CC1 OMEBKMLPDOPNNC-UHFFFAOYSA-N 0.000 description 1
• PHUZEQBSANCEHJ-UHFFFAOYSA-N 3-methyl-5-phenylpyrazin-2-amine Chemical compound N1=C(N)C(C)=NC(C=2C=CC=CC=2)=C1 PHUZEQBSANCEHJ-UHFFFAOYSA-N 0.000 description 1
• HZNLSEFDJFDNFU-UHFFFAOYSA-N 3-phenylpyrazin-2-amine Chemical compound NC1=NC=CN=C1C1=CC=CC=C1 HZNLSEFDJFDNFU-UHFFFAOYSA-N 0.000 description 1
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
• 125000004172 4-methoxyphenyl group Chemical group [H]C1=C([H])C(OC([H])([H])[H])=C([H])C([H])=C1* 0.000 description 1
• 102220615486 40S ribosomal protein S13_K43N_mutation Human genes 0.000 description 1
• BSRMZHHSEUOXMH-UHFFFAOYSA-N 6-(4-hydroxyphenyl)-2-[(4-hydroxyphenyl)methyl]-7h-imidazo[1,2-a]pyrazin-3-one Chemical compound C1=CC(O)=CC=C1CC1=NC2=CNC(C=3C=CC(O)=CC=3)=CN2C1=O BSRMZHHSEUOXMH-UHFFFAOYSA-N 0.000 description 1
• WJOLQGAMGUBOFS-UHFFFAOYSA-N 8-(cyclopentylmethyl)-2-[(4-fluorophenyl)methyl]-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3-ol Chemical compound Oc1c(Cc2ccc(F)cc2)nc2c(CC3CCCC3)nc(cn12)-c1ccc(O)cc1 WJOLQGAMGUBOFS-UHFFFAOYSA-N 0.000 description 1
• YBLMZJSGNQTCLU-UHFFFAOYSA-N 8-(cyclopentylmethyl)-6-(4-hydroxyphenyl)-2-[(4-hydroxyphenyl)methyl]imidazo[1,2-a]pyrazin-3-ol Chemical compound Oc1c(Cc2ccc(O)cc2)nc2c(CC3CCCC3)nc(cn12)-c1ccc(O)cc1 YBLMZJSGNQTCLU-UHFFFAOYSA-N 0.000 description 1
• DZXCSPIUFRYXDZ-UHFFFAOYSA-N 8-benzyl-2-(1h-imidazol-2-ylmethyl)-6-phenyl-7h-imidazo[1,2-a]pyrazin-3-one Chemical compound N=1C2=C(CC=3C=CC=CC=3)NC(C=3C=CC=CC=3)=CN2C(=O)C=1CC1=NC=CN1 DZXCSPIUFRYXDZ-UHFFFAOYSA-N 0.000 description 1
• MIEOMFFJYLPPAT-UHFFFAOYSA-N 8-benzyl-2-[(1-methylimidazol-2-yl)methyl]-6-phenyl-7h-imidazo[1,2-a]pyrazin-3-one Chemical compound CN1C=CN=C1CC(C(N1C=C(N2)C=3C=CC=CC=3)=O)=NC1=C2CC1=CC=CC=C1 MIEOMFFJYLPPAT-UHFFFAOYSA-N 0.000 description 1
• MEMQQZHHXCOKGG-UHFFFAOYSA-N 8-benzyl-2-[(4-fluorophenyl)methyl]-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3-ol Chemical compound Oc1c(Cc2ccc(F)cc2)nc2c(Cc3ccccc3)nc(cn12)-c1ccc(O)cc1 MEMQQZHHXCOKGG-UHFFFAOYSA-N 0.000 description 1
• OCFIMLDSLWGMMR-UHFFFAOYSA-N 8-benzyl-2-butyl-6-(4-hydroxyphenyl)-7h-imidazo[1,2-a]pyrazin-3-one Chemical compound N1C(C=2C=CC(O)=CC=2)=CN2C(=O)C(CCCC)=NC2=C1CC1=CC=CC=C1 OCFIMLDSLWGMMR-UHFFFAOYSA-N 0.000 description 1
• VKCSMYMCRSXVID-UHFFFAOYSA-N 8-benzyl-2-ethyl-6-phenyl-7h-imidazo[1,2-a]pyrazin-3-one Chemical compound N1C(C=2C=CC=CC=2)=CN2C(=O)C(CC)=NC2=C1CC1=CC=CC=C1 VKCSMYMCRSXVID-UHFFFAOYSA-N 0.000 description 1
• ONVKEAHBFKWZHK-UHFFFAOYSA-N 8-benzyl-6-(4-hydroxyphenyl)-2-(naphthalen-1-ylmethyl)imidazo[1,2-a]pyrazin-3-ol Chemical compound Oc1c(Cc2cccc3ccccc23)nc2c(Cc3ccccc3)nc(cn12)-c1ccc(O)cc1 ONVKEAHBFKWZHK-UHFFFAOYSA-N 0.000 description 1
• FFSPSFRJIWKBNR-UHFFFAOYSA-N 8-benzyl-6-(4-hydroxyphenyl)-2-[(1-methylimidazol-2-yl)methyl]-6-phenyl-5,7-dihydroimidazo[1,2-a]pyrazin-3-one Chemical compound CN1C=CN=C1CC(C(N1CC(N2)(C=3C=CC=CC=3)C=3C=CC(O)=CC=3)=O)=NC1=C2CC1=CC=CC=C1 FFSPSFRJIWKBNR-UHFFFAOYSA-N 0.000 description 1
• SBYVQABZMXOXNM-UHFFFAOYSA-N 8-benzyl-6-phenyl-2-(3,3,3-trifluoropropyl)-7h-imidazo[1,2-a]pyrazin-3-one Chemical compound N1C(C=2C=CC=CC=2)=CN2C(=O)C(CCC(F)(F)F)=NC2=C1CC1=CC=CC=C1 SBYVQABZMXOXNM-UHFFFAOYSA-N 0.000 description 1
• WSNMZQDNOGOIRD-UHFFFAOYSA-N 8-benzyl-6-phenyl-2-(thiophen-2-ylmethyl)-7h-imidazo[1,2-a]pyrazin-3-one Chemical compound N=1C2=C(CC=3C=CC=CC=3)NC(C=3C=CC=CC=3)=CN2C(=O)C=1CC1=CC=CS1 WSNMZQDNOGOIRD-UHFFFAOYSA-N 0.000 description 1
• JLZNOCIFFOJCJF-UHFFFAOYSA-N 8-cyclopropyl-2-[(4-hydroxyphenyl)methyl]-6-phenyl-7h-imidazo[1,2-a]pyrazin-3-one Chemical compound C1=CC(O)=CC=C1CC1=NC2=C(C3CC3)NC(C=3C=CC=CC=3)=CN2C1=O JLZNOCIFFOJCJF-UHFFFAOYSA-N 0.000 description 1
• 241000243290 Aequorea Species 0.000 description 1
• 108010000239 Aequorin Proteins 0.000 description 1
• NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical class [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 1
• QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
• 241000193830 Bacillus <bacterium> Species 0.000 description 1
• 108010016529 Bacillus amyloliquefaciens ribonuclease Proteins 0.000 description 1
• 108090000363 Bacterial Luciferases Proteins 0.000 description 1
• 235000016068 Berberis vulgaris Nutrition 0.000 description 1
• 241000335053 Beta vulgaris Species 0.000 description 1
• COVZYZSDYWQREU-UHFFFAOYSA-N Busulfan Chemical compound CS(=O)(=O)OCCCCOS(C)(=O)=O COVZYZSDYWQREU-UHFFFAOYSA-N 0.000 description 1
• 101150003532 CSH gene Proteins 0.000 description 1
• 241000282465 Canis Species 0.000 description 1
• 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
• 102000014914 Carrier Proteins Human genes 0.000 description 1
• 102000003952 Caspase 3 Human genes 0.000 description 1
• 108090000397 Caspase 3 Proteins 0.000 description 1
• 241000238366 Cephalopoda Species 0.000 description 1
• KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
• 241000243321 Cnidaria Species 0.000 description 1
• IVOMOUWHDPKRLL-KQYNXXCUSA-N Cyclic adenosine monophosphate Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-KQYNXXCUSA-N 0.000 description 1
• 241000035538 Cypridina Species 0.000 description 1
• 102220541230 Cystatin-D_H86R_mutation Human genes 0.000 description 1
• 102220603804 Cytosolic iron-sulfur assembly component 3_E49D_mutation Human genes 0.000 description 1
• 102220585510 D site-binding protein_S66T_mutation Human genes 0.000 description 1
• 241000238557 Decapoda Species 0.000 description 1
• 102000007260 Deoxyribonuclease I Human genes 0.000 description 1
• 108010008532 Deoxyribonuclease I Proteins 0.000 description 1
• 101100125027 Dictyostelium discoideum mhsp70 gene Proteins 0.000 description 1
• XBPCUCUWBYBCDP-UHFFFAOYSA-N Dicyclohexylamine Chemical compound C1CCCCC1NC1CCCCC1 XBPCUCUWBYBCDP-UHFFFAOYSA-N 0.000 description 1
• MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
• OTMSDBZUPAUEDD-UHFFFAOYSA-N Ethane Chemical compound CC OTMSDBZUPAUEDD-UHFFFAOYSA-N 0.000 description 1
• JOYRKODLDBILNP-UHFFFAOYSA-N Ethyl urethane Chemical compound CCOC(N)=O JOYRKODLDBILNP-UHFFFAOYSA-N 0.000 description 1
• PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 description 1
• 239000001263 FEMA 3042 Substances 0.000 description 1
• 102220568144 Fatty acid-binding protein 5_L92A_mutation Human genes 0.000 description 1
• 102100026748 Fatty acid-binding protein, intestinal Human genes 0.000 description 1
• 241000282324 Felis Species 0.000 description 1
• 102000003688 G-Protein-Coupled Receptors Human genes 0.000 description 1
• 108090000045 G-Protein-Coupled Receptors Proteins 0.000 description 1
• 102220621888 G-protein coupled estrogen receptor 1_L18Q_mutation Human genes 0.000 description 1
• 108010010803 Gelatin Proteins 0.000 description 1
• JRZJKWGQFNTSRN-UHFFFAOYSA-N Geldanamycin Natural products C1C(C)CC(OC)C(O)C(C)C=C(C)C(OC(N)=O)C(OC)CCC=C(C)C(=O)NC2=CC(=O)C(OC)=C1C2=O JRZJKWGQFNTSRN-UHFFFAOYSA-N 0.000 description 1
• 108700028146 Genetic Enhancer Elements Proteins 0.000 description 1
• 102000005720 Glutathione transferase Human genes 0.000 description 1
• 108010070675 Glutathione transferase Proteins 0.000 description 1
• 101150031823 HSP70 gene Proteins 0.000 description 1
• 102220607936 Hemoglobin subunit beta_V68D_mutation Human genes 0.000 description 1
• 108700005087 Homeobox Genes Proteins 0.000 description 1
• 241000282412 Homo Species 0.000 description 1
• 101100066148 Homo sapiens FABP1 gene Proteins 0.000 description 1
• 101000911337 Homo sapiens Fatty acid-binding protein, intestinal Proteins 0.000 description 1
• 101000911317 Homo sapiens Fatty acid-binding protein, liver Proteins 0.000 description 1
• 101000984042 Homo sapiens Protein lin-28 homolog A Proteins 0.000 description 1
• DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
• 108091092195 Intron Proteins 0.000 description 1
• FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
• 241000131894 Lampyris noctiluca Species 0.000 description 1
• 241000209510 Liliopsida Species 0.000 description 1
• GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
• 102220526291 N-acetylneuraminate lyase_Y94F_mutation Human genes 0.000 description 1
• MBBZMMPHUWSWHV-BDVNFPICSA-N N-methylglucamine Chemical compound CNC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO MBBZMMPHUWSWHV-BDVNFPICSA-N 0.000 description 1
• 150000001204 N-oxides Chemical class 0.000 description 1
• 108010057466 NF-kappa B Proteins 0.000 description 1
• 102000003945 NF-kappa B Human genes 0.000 description 1
• VYEAHXRPWKOEMY-UHFFFAOYSA-N O-(9H-fluoren-9-ylmethyl)hydroxylamine Chemical compound C1=CC=C2C(CON)C3=CC=CC=C3C2=C1 VYEAHXRPWKOEMY-UHFFFAOYSA-N 0.000 description 1
• BHVRCUAHXVLSNX-UHFFFAOYSA-N O-[(4,5-dimethoxy-2-nitrophenyl)methyl]hydroxylamine Chemical compound COC1=CC(CON)=C([N+]([O-])=O)C=C1OC BHVRCUAHXVLSNX-UHFFFAOYSA-N 0.000 description 1
• 102220492844 Olfactory receptor 8K5_F68S_mutation Human genes 0.000 description 1
• 108091034117 Oligonucleotide Proteins 0.000 description 1
• 102220520619 Origin recognition complex subunit 2_M106K_mutation Human genes 0.000 description 1
• 102220513989 PCI domain-containing protein 2_D55E_mutation Human genes 0.000 description 1
• 239000007990 PIPES buffer Substances 0.000 description 1
• LRBQNJMCXXYXIU-PPKXGCFTSA-N Penta-digallate-beta-D-glucose Natural products OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-PPKXGCFTSA-N 0.000 description 1
• 102100022239 Peroxiredoxin-6 Human genes 0.000 description 1
• 108091000080 Phosphotransferase Proteins 0.000 description 1
• 241001505950 Photuris pensylvanica Species 0.000 description 1
• 241000360553 Phrixothrix hirtus Species 0.000 description 1
• 241000235648 Pichia Species 0.000 description 1
• 101800001016 Picornain 3C-like protease Proteins 0.000 description 1
• 102220553081 Proenkephalin-A_F77A_mutation Human genes 0.000 description 1
• ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
• 102000001253 Protein Kinase Human genes 0.000 description 1
• 102100024924 Protein kinase C alpha type Human genes 0.000 description 1
• 101710109947 Protein kinase C alpha type Proteins 0.000 description 1
• 102100025460 Protein lin-28 homolog A Human genes 0.000 description 1
• 241001558063 Pyrearinus termitilluminans Species 0.000 description 1
• 241001427618 Pyrophorus plagiophthalamus Species 0.000 description 1
• 108091030071 RNAI Proteins 0.000 description 1
• NPXOKRUENSOPAO-UHFFFAOYSA-N Raney nickel Chemical compound [Al].[Ni] NPXOKRUENSOPAO-UHFFFAOYSA-N 0.000 description 1
• 241000242743 Renilla reniformis Species 0.000 description 1
• 108010083644 Ribonucleases Proteins 0.000 description 1
• 102000006382 Ribonucleases Human genes 0.000 description 1
• 241000235070 Saccharomyces Species 0.000 description 1
• 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
• 241000235346 Schizosaccharomyces Species 0.000 description 1
• 108091081021 Sense strand Proteins 0.000 description 1
• JXVIIQLNUPXOII-UHFFFAOYSA-N Siduron Chemical compound CC1CCCCC1NC(=O)NC1=CC=CC=C1 JXVIIQLNUPXOII-UHFFFAOYSA-N 0.000 description 1
• 108091027967 Small hairpin RNA Proteins 0.000 description 1
• 108020004459 Small interfering RNA Proteins 0.000 description 1
• CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical class [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
• 241000191940 Staphylococcus Species 0.000 description 1
• 108010090804 Streptavidin Proteins 0.000 description 1
• 241000187747 Streptomyces Species 0.000 description 1
• 101100482220 Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) triC gene Proteins 0.000 description 1
• BGNXCDMCOKJUMV-UHFFFAOYSA-N Tert-Butylhydroquinone Chemical compound CC(C)(C)C1=CC(O)=CC=C1O BGNXCDMCOKJUMV-UHFFFAOYSA-N 0.000 description 1
• 102000006601 Thymidine Kinase Human genes 0.000 description 1
• 108020004440 Thymidine kinase Proteins 0.000 description 1
• 229910003074 TiCl4 Inorganic materials 0.000 description 1
• RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 1
• 241000723792 Tobacco etch virus Species 0.000 description 1
• 101710120037 Toxin CcdB Proteins 0.000 description 1
• 208000034953 Twin anemia-polycythemia sequence Diseases 0.000 description 1
• IVOMOUWHDPKRLL-UHFFFAOYSA-N UNPD107823 Natural products O1C2COP(O)(=O)OC2C(O)C1N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-UHFFFAOYSA-N 0.000 description 1
• 241000238584 Vargula Species 0.000 description 1
• 241000700605 Viruses Species 0.000 description 1
• 241000238429 Watasenia scintillans Species 0.000 description 1
• HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
• INULNSAIIZKOQE-YOSAUDMPSA-N [(3r,4ar,10ar)-6-methoxy-1-methyl-3,4,4a,5,10,10a-hexahydro-2h-benzo[g]quinolin-3-yl]-[4-(4-nitrophenyl)piperazin-1-yl]methanone Chemical compound O=C([C@@H]1C[C@H]2[C@H](N(C1)C)CC=1C=CC=C(C=1C2)OC)N(CC1)CCN1C1=CC=C([N+]([O-])=O)C=C1 INULNSAIIZKOQE-YOSAUDMPSA-N 0.000 description 1
• DGEZNRSVGBDHLK-UHFFFAOYSA-N [1,10]phenanthroline Chemical compound C1=CN=C2C3=NC=CC=C3C=CC2=C1 DGEZNRSVGBDHLK-UHFFFAOYSA-N 0.000 description 1
• DHKHKXVYLBGOIT-UHFFFAOYSA-N acetaldehyde Diethyl Acetal Natural products CCOC(C)OCC DHKHKXVYLBGOIT-UHFFFAOYSA-N 0.000 description 1
• 150000001241 acetals Chemical class 0.000 description 1
• 239000000556 agonist Substances 0.000 description 1
• 229910001413 alkali metal ion Inorganic materials 0.000 description 1
• 230000008841 allosteric interaction Effects 0.000 description 1
• 150000004716 alpha keto acids Chemical class 0.000 description 1
• 108010084650 alpha-N-arabinofuranosidase Proteins 0.000 description 1
• HSFWRNGVRCDJHI-UHFFFAOYSA-N alpha-acetylene Natural products C#C HSFWRNGVRCDJHI-UHFFFAOYSA-N 0.000 description 1
• 150000001370 alpha-amino acid derivatives Chemical class 0.000 description 1
• 235000008206 alpha-amino acids Nutrition 0.000 description 1
• 125000000539 amino acid group Chemical group 0.000 description 1
• 125000003277 amino group Chemical group 0.000 description 1
• 125000005219 aminonitrile group Chemical group 0.000 description 1
• 238000004873 anchoring Methods 0.000 description 1
• 150000001449 anionic compounds Chemical class 0.000 description 1
• 230000000890 antigenic effect Effects 0.000 description 1
• 239000003963 antioxidant agent Substances 0.000 description 1
• 230000003078 antioxidant effect Effects 0.000 description 1
• 238000003782 apoptosis assay Methods 0.000 description 1
• 239000007900 aqueous suspension Substances 0.000 description 1
• 229910052786 argon Inorganic materials 0.000 description 1
• 238000011914 asymmetric synthesis Methods 0.000 description 1
• 239000012298 atmosphere Substances 0.000 description 1
• QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
• WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid group Chemical group C(C1=CC=CC=C1)(=O)O WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 1
• RWCCWEUUXYIKHB-UHFFFAOYSA-N benzophenone Chemical compound C=1C=CC=CC=1C(=O)C1=CC=CC=C1 RWCCWEUUXYIKHB-UHFFFAOYSA-N 0.000 description 1
• 239000012965 benzophenone Substances 0.000 description 1
• 102000005936 beta-Galactosidase Human genes 0.000 description 1
• 108010005774 beta-Galactosidase Proteins 0.000 description 1
• GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
• 108091008324 binding proteins Proteins 0.000 description 1
• 239000012455 biphasic mixture Substances 0.000 description 1
• 230000000903 blocking effect Effects 0.000 description 1
• 210000004369 blood Anatomy 0.000 description 1
• 239000008280 blood Substances 0.000 description 1
• 229910052794 bromium Inorganic materials 0.000 description 1
• 244000309464 bull Species 0.000 description 1
• 125000004369 butenyl group Chemical group C(=CCC)* 0.000 description 1
• 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
• 102220452443 c.137T>A Human genes 0.000 description 1
• 102220362001 c.164A>G Human genes 0.000 description 1
• 102220355440 c.257A>T Human genes 0.000 description 1
• 102220429258 c.283G>A Human genes 0.000 description 1
• 102220405930 c.41C>T Human genes 0.000 description 1
• 102220348816 c.59A>C Human genes 0.000 description 1
• 102220423675 c.96G>T Human genes 0.000 description 1
• 150000001718 carbodiimides Chemical class 0.000 description 1
• 125000004432 carbon atom Chemical group C* 0.000 description 1
• 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 1
• 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
• 239000001768 carboxy methyl cellulose Substances 0.000 description 1
• 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
• 125000002843 carboxylic acid group Chemical group 0.000 description 1
• 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
• 230000015556 catabolic process Effects 0.000 description 1
• 239000003054 catalyst Substances 0.000 description 1
• 150000001767 cationic compounds Chemical class 0.000 description 1
• 238000004113 cell culture Methods 0.000 description 1
• 210000000170 cell membrane Anatomy 0.000 description 1
• 230000033077 cellular process Effects 0.000 description 1
• GGRHYQCXXYLUTL-UHFFFAOYSA-N chloromethyl 2,2-dimethylpropanoate Chemical compound CC(C)(C)C(=O)OCCl GGRHYQCXXYLUTL-UHFFFAOYSA-N 0.000 description 1
• OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 description 1
• 229960001231 choline Drugs 0.000 description 1
• LSSJELUXTIYYDZ-UHFFFAOYSA-N coelenterazine e Chemical compound C1=CC(O)=CC=C1CC(C(N1C=2CCC3=CC(O)=CC=C3C=2N2)=O)=NC1=C2CC1=CC=CC=C1 LSSJELUXTIYYDZ-UHFFFAOYSA-N 0.000 description 1
• 230000002860 competitive effect Effects 0.000 description 1
• 239000002299 complementary DNA Substances 0.000 description 1
• 229940125904 compound 1 Drugs 0.000 description 1
• 229940125782 compound 2 Drugs 0.000 description 1
• 238000000205 computational method Methods 0.000 description 1
• 238000009833 condensation Methods 0.000 description 1
• 230000005494 condensation Effects 0.000 description 1
• 238000012790 confirmation Methods 0.000 description 1
• 239000000356 contaminant Substances 0.000 description 1
• 229940095074 cyclic amp Drugs 0.000 description 1
• 125000004122 cyclic group Chemical group 0.000 description 1
• 125000001047 cyclobutenyl group Chemical group C1(=CCC1)* 0.000 description 1
• 125000001995 cyclobutyl group Chemical group [H]C1([H])C([H])([H])C([H])(*)C1([H])[H] 0.000 description 1
• 125000002433 cyclopentenyl group Chemical group C1(=CCCC1)* 0.000 description 1
• 125000001511 cyclopentyl group Chemical group [H]C1([H])C([H])([H])C([H])([H])C([H])(*)C1([H])[H] 0.000 description 1
• 125000000298 cyclopropenyl group Chemical group [H]C1=C([H])C1([H])* 0.000 description 1
• 125000001559 cyclopropyl group Chemical group [H]C1([H])C([H])([H])C1([H])* 0.000 description 1
• 238000000354 decomposition reaction Methods 0.000 description 1
• 238000006731 degradation reaction Methods 0.000 description 1
• 230000001627 detrimental effect Effects 0.000 description 1
• AQEFLFZSWDEAIP-UHFFFAOYSA-N di-tert-butyl ether Chemical compound CC(C)(C)OC(C)(C)C AQEFLFZSWDEAIP-UHFFFAOYSA-N 0.000 description 1
• ZBCBWPMODOFKDW-UHFFFAOYSA-N diethanolamine Chemical compound OCCNCCO ZBCBWPMODOFKDW-UHFFFAOYSA-N 0.000 description 1
• WYACBZDAHNBPPB-UHFFFAOYSA-N diethyl oxalate Chemical compound CCOC(=O)C(=O)OCC WYACBZDAHNBPPB-UHFFFAOYSA-N 0.000 description 1
• HPNMFZURTQLUMO-UHFFFAOYSA-N diethylamine Chemical compound CCNCC HPNMFZURTQLUMO-UHFFFAOYSA-N 0.000 description 1
• SBZXBUIDTXKZTM-UHFFFAOYSA-N diglyme Chemical compound COCCOCCOC SBZXBUIDTXKZTM-UHFFFAOYSA-N 0.000 description 1
• 238000007865 diluting Methods 0.000 description 1
• 229910001882 dioxygen Inorganic materials 0.000 description 1
• ZZVUWRFHKOJYTH-UHFFFAOYSA-N diphenhydramine Chemical group C=1C=CC=CC=1C(OCCN(C)C)C1=CC=CC=C1 ZZVUWRFHKOJYTH-UHFFFAOYSA-N 0.000 description 1
• 125000005982 diphenylmethyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])(*)C1=C([H])C([H])=C([H])C([H])=C1[H] 0.000 description 1
• 101150052825 dnaK gene Proteins 0.000 description 1
• 229940079593 drug Drugs 0.000 description 1
• 239000003814 drug Substances 0.000 description 1
• 238000001035 drying Methods 0.000 description 1
• 238000004520 electroporation Methods 0.000 description 1
• 238000000295 emission spectrum Methods 0.000 description 1
• 239000000839 emulsion Substances 0.000 description 1
• 150000002081 enamines Chemical class 0.000 description 1
• 125000002587 enol group Chemical group 0.000 description 1
• 230000007613 environmental effect Effects 0.000 description 1
• 238000006911 enzymatic reaction Methods 0.000 description 1
• 239000003248 enzyme activator Substances 0.000 description 1
• 229940125532 enzyme inhibitor Drugs 0.000 description 1
• 235000020776 essential amino acid Nutrition 0.000 description 1
• 239000003797 essential amino acid Substances 0.000 description 1
• FPULFENIJDPZBX-UHFFFAOYSA-N ethyl 2-isocyanoacetate Chemical compound CCOC(=O)C[N+]#[C-] FPULFENIJDPZBX-UHFFFAOYSA-N 0.000 description 1
• TXTNQKFBDXJERM-UHFFFAOYSA-N ethyl 3-(furan-2-yl)-2-oxopropanoate Chemical compound CCOC(=O)C(=O)CC1=CC=CO1 TXTNQKFBDXJERM-UHFFFAOYSA-N 0.000 description 1
• PSRZMXNNQTWAGB-UHFFFAOYSA-N ethyl 4,4,4-trifluorobutanoate Chemical compound CCOC(=O)CCC(F)(F)F PSRZMXNNQTWAGB-UHFFFAOYSA-N 0.000 description 1
• 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
• 125000002534 ethynyl group Chemical group [H]C#C* 0.000 description 1
• 241001233957 eudicotyledons Species 0.000 description 1
• 230000001747 exhibiting effect Effects 0.000 description 1
• 239000013604 expression vector Substances 0.000 description 1
• 230000002349 favourable effect Effects 0.000 description 1
• 108010062616 filensin Proteins 0.000 description 1
• 239000012530 fluid Substances 0.000 description 1
• GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
• KCPRYVGBEBFLIG-UHFFFAOYSA-N fluorescein bis-arsenide Chemical compound OC(=O)C1=CC=CC=C1C1=C2C=CC(=O)C([As]3SCCS3)=C2OC2=C([As]3SCCS3)C(O)=CC=C21 KCPRYVGBEBFLIG-UHFFFAOYSA-N 0.000 description 1
• 229910052731 fluorine Inorganic materials 0.000 description 1
• CNUDBTRUORMMPA-UHFFFAOYSA-N formylthiophene Chemical compound O=CC1=CC=CS1 CNUDBTRUORMMPA-UHFFFAOYSA-N 0.000 description 1
• 238000001640 fractional crystallisation Methods 0.000 description 1
• 239000012737 fresh medium Substances 0.000 description 1
• 238000010230 functional analysis Methods 0.000 description 1
• 125000002541 furyl group Chemical group 0.000 description 1
• 239000008273 gelatin Substances 0.000 description 1
• 229920000159 gelatin Polymers 0.000 description 1
• 235000019322 gelatine Nutrition 0.000 description 1
• 235000011852 gelatine desserts Nutrition 0.000 description 1
• QTQAWLPCGQOSGP-GBTDJJJQSA-N geldanamycin Chemical compound N1C(=O)\C(C)=C/C=C\[C@@H](OC)[C@H](OC(N)=O)\C(C)=C/[C@@H](C)[C@@H](O)[C@H](OC)C[C@@H](C)CC2=C(OC)C(=O)C=C1C2=O QTQAWLPCGQOSGP-GBTDJJJQSA-N 0.000 description 1
• 230000009368 gene silencing by RNA Effects 0.000 description 1
• 230000004034 genetic regulation Effects 0.000 description 1
• BRZYSWJRSDMWLG-CAXSIQPQSA-N geneticin Natural products O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](C(C)O)O2)N)[C@@H](N)C[C@H]1N BRZYSWJRSDMWLG-CAXSIQPQSA-N 0.000 description 1
• 230000013595 glycosylation Effects 0.000 description 1
• 238000006206 glycosylation reaction Methods 0.000 description 1
• 229940015043 glyoxal Drugs 0.000 description 1
• 229940093915 gynecological organic acid Drugs 0.000 description 1
• 229910052736 halogen Inorganic materials 0.000 description 1
• 150000002367 halogens Chemical class 0.000 description 1
• 238000013537 high throughput screening Methods 0.000 description 1
• 238000000703 high-speed centrifugation Methods 0.000 description 1
• HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
• 229940088597 hormone Drugs 0.000 description 1
• 239000005556 hormone Substances 0.000 description 1
• 229940091173 hydantoin Drugs 0.000 description 1
• BHEPBYXIRTUNPN-UHFFFAOYSA-N hydridophosphorus(.) (triplet) Chemical compound [PH] BHEPBYXIRTUNPN-UHFFFAOYSA-N 0.000 description 1
• 238000005984 hydrogenation reaction Methods 0.000 description 1
• 230000007062 hydrolysis Effects 0.000 description 1
• 238000006460 hydrolysis reaction Methods 0.000 description 1
• 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
• UWYVPFMHMJIBHE-OWOJBTEDSA-N hydroxymaleic acid group Chemical group O/C(/C(=O)O)=C/C(=O)O UWYVPFMHMJIBHE-OWOJBTEDSA-N 0.000 description 1
• 125000004029 hydroxymethyl group Chemical group [H]OC([H])([H])* 0.000 description 1
• 238000003018 immunoassay Methods 0.000 description 1
• 238000001114 immunoprecipitation Methods 0.000 description 1
• 238000000338 in vitro Methods 0.000 description 1
• 230000002779 inactivation Effects 0.000 description 1
• 238000010348 incorporation Methods 0.000 description 1
• 230000001939 inductive effect Effects 0.000 description 1
• 230000002401 inhibitory effect Effects 0.000 description 1
• 230000000977 initiatory effect Effects 0.000 description 1
• 229910001412 inorganic anion Inorganic materials 0.000 description 1
• 229910001411 inorganic cation Inorganic materials 0.000 description 1
• 230000031146 intracellular signal transduction Effects 0.000 description 1
• 229910052740 iodine Inorganic materials 0.000 description 1
• OWFXIOWLTKNBAP-UHFFFAOYSA-N isoamyl nitrite Chemical compound CC(C)CCON=O OWFXIOWLTKNBAP-UHFFFAOYSA-N 0.000 description 1
• 238000002955 isolation Methods 0.000 description 1
• 125000001972 isopentyl group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])C([H])([H])* 0.000 description 1
• 238000003367 kinetic assay Methods 0.000 description 1
• 238000002372 labelling Methods 0.000 description 1
• 231100001231 less toxic Toxicity 0.000 description 1
• 150000002614 leucines Chemical class 0.000 description 1
• 230000000670 limiting effect Effects 0.000 description 1
• 238000001638 lipofection Methods 0.000 description 1
• 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 1
• 238000011068 loading method Methods 0.000 description 1
• 230000004807 localization Effects 0.000 description 1
• 238000000504 luminescence detection Methods 0.000 description 1
• 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
• 235000019341 magnesium sulphate Nutrition 0.000 description 1
• 230000000873 masking effect Effects 0.000 description 1
• 230000001404 mediated effect Effects 0.000 description 1
• 229960003194 meglumine Drugs 0.000 description 1
• 229910052751 metal Inorganic materials 0.000 description 1
• 239000002184 metal Substances 0.000 description 1
• MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
• 150000004702 methyl esters Chemical class 0.000 description 1
• 108091070501 miRNA Proteins 0.000 description 1
• 239000002679 microRNA Substances 0.000 description 1
• 238000000520 microinjection Methods 0.000 description 1
• 238000000386 microscopy Methods 0.000 description 1
• 150000007522 mineralic acids Chemical class 0.000 description 1
• 238000010369 molecular cloning Methods 0.000 description 1
• 239000002808 molecular sieve Substances 0.000 description 1
• 150000004682 monohydrates Chemical class 0.000 description 1
• 238000002703 mutagenesis Methods 0.000 description 1
• 231100000350 mutagenesis Toxicity 0.000 description 1
• 231100000219 mutagenic Toxicity 0.000 description 1
• 230000003505 mutagenic effect Effects 0.000 description 1
• PSHKMPUSSFXUIA-UHFFFAOYSA-N n,n-dimethylpyridin-2-amine Chemical compound CN(C)C1=CC=CC=N1 PSHKMPUSSFXUIA-UHFFFAOYSA-N 0.000 description 1
• MRHQBBSKCLIMRH-UHFFFAOYSA-N n-(acetamidomethoxymethyl)acetamide Chemical compound CC(=O)NCOCNC(C)=O MRHQBBSKCLIMRH-UHFFFAOYSA-N 0.000 description 1
• SYSQUGFVNFXIIT-UHFFFAOYSA-N n-[4-(1,3-benzoxazol-2-yl)phenyl]-4-nitrobenzenesulfonamide Chemical class C1=CC([N+](=O)[O-])=CC=C1S(=O)(=O)NC1=CC=C(C=2OC3=CC=CC=C3N=2)C=C1 SYSQUGFVNFXIIT-UHFFFAOYSA-N 0.000 description 1
• GTWJETSWSUWSEJ-UHFFFAOYSA-N n-benzylaniline Chemical compound C=1C=CC=CC=1CNC1=CC=CC=C1 GTWJETSWSUWSEJ-UHFFFAOYSA-N 0.000 description 1
• 125000004108 n-butyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
• 125000001280 n-hexyl group Chemical group C(CCCCC)* 0.000 description 1
• 239000002105 nanoparticle Substances 0.000 description 1
• 230000007935 neutral effect Effects 0.000 description 1
• GQPLMRYTRLFLPF-UHFFFAOYSA-N nitrous oxide Inorganic materials [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 description 1
• 238000010606 normalization Methods 0.000 description 1
• 238000007899 nucleic acid hybridization Methods 0.000 description 1
• LUHFJLLCZSYACL-UHFFFAOYSA-N o-(2,2,2-trichloroethyl)hydroxylamine Chemical compound NOCC(Cl)(Cl)Cl LUHFJLLCZSYACL-UHFFFAOYSA-N 0.000 description 1
• GWCBVFMHGHMALR-UHFFFAOYSA-N o-(2-trimethylsilylethyl)hydroxylamine Chemical compound C[Si](C)(C)CCON GWCBVFMHGHMALR-UHFFFAOYSA-N 0.000 description 1
• XYEOALKITRFCJJ-UHFFFAOYSA-N o-benzylhydroxylamine Chemical compound NOCC1=CC=CC=C1 XYEOALKITRFCJJ-UHFFFAOYSA-N 0.000 description 1
• KKVUFSINQFSJNK-UHFFFAOYSA-N o-tert-butylhydroxylamine Chemical compound CC(C)(C)ON KKVUFSINQFSJNK-UHFFFAOYSA-N 0.000 description 1
• 108010089433 obelin Proteins 0.000 description 1
• 230000003287 optical effect Effects 0.000 description 1
• 150000007524 organic acids Chemical class 0.000 description 1
• 235000005985 organic acids Nutrition 0.000 description 1
• 150000002891 organic anions Chemical class 0.000 description 1
• 150000002892 organic cations Chemical class 0.000 description 1
• 239000012074 organic phase Substances 0.000 description 1
• 230000002018 overexpression Effects 0.000 description 1
• 150000002923 oximes Chemical class 0.000 description 1
• 239000001301 oxygen Substances 0.000 description 1
• 229940094443 oxytocics prostaglandins Drugs 0.000 description 1
• IPCSVZSSVZVIGE-UHFFFAOYSA-N palmitic acid group Chemical group C(CCCCCCCCCCCCCCC)(=O)O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 1
• 210000000496 pancreas Anatomy 0.000 description 1
• RUVINXPYWBROJD-UHFFFAOYSA-N para-methoxyphenyl Natural products COC1=CC=C(C=CC)C=C1 RUVINXPYWBROJD-UHFFFAOYSA-N 0.000 description 1
• 238000010647 peptide synthesis reaction Methods 0.000 description 1
• 229940100595 phenylacetaldehyde Drugs 0.000 description 1
• WLJVXDMOQOGPHL-UHFFFAOYSA-N phenylacetic acid Chemical compound OC(=O)CC1=CC=CC=C1 WLJVXDMOQOGPHL-UHFFFAOYSA-N 0.000 description 1
• PHEDXBVPIONUQT-RGYGYFBISA-N phorbol 13-acetate 12-myristate Chemical compound C([C@]1(O)C(=O)C(C)=C[C@H]1[C@@]1(O)[C@H](C)[C@H]2OC(=O)CCCCCCCCCCCCC)C(CO)=C[C@H]1[C@H]1[C@]2(OC(C)=O)C1(C)C PHEDXBVPIONUQT-RGYGYFBISA-N 0.000 description 1
• 239000002644 phorbol ester Substances 0.000 description 1
• 230000026731 phosphorylation Effects 0.000 description 1
• 238000006366 phosphorylation reaction Methods 0.000 description 1
• 102000020233 phosphotransferase Human genes 0.000 description 1
• 230000035479 physiological effects, processes and functions Effects 0.000 description 1
• 210000002381 plasma Anatomy 0.000 description 1
• 239000013600 plasmid vector Substances 0.000 description 1
• 239000013641 positive control Substances 0.000 description 1
• 230000001124 posttranscriptional effect Effects 0.000 description 1
• 229910052700 potassium Inorganic materials 0.000 description 1
• 229910000027 potassium carbonate Inorganic materials 0.000 description 1
• BDAWXSQJJCIFIK-UHFFFAOYSA-N potassium methoxide Chemical compound [K+].[O-]C BDAWXSQJJCIFIK-UHFFFAOYSA-N 0.000 description 1
• 239000002244 precipitate Substances 0.000 description 1
• 238000001556 precipitation Methods 0.000 description 1
• 239000002243 precursor Substances 0.000 description 1
• 150000003138 primary alcohols Chemical class 0.000 description 1
• 230000009465 prokaryotic expression Effects 0.000 description 1
• 150000003180 prostaglandins Chemical class 0.000 description 1
• 235000004252 protein component Nutrition 0.000 description 1
• 108060006633 protein kinase Proteins 0.000 description 1
• 230000012743 protein tagging Effects 0.000 description 1
• 230000007398 protein translocation Effects 0.000 description 1
• 150000003216 pyrazines Chemical class 0.000 description 1
• 238000006862 quantum yield reaction Methods 0.000 description 1
• 125000001453 quaternary ammonium group Chemical group 0.000 description 1
• 230000000171 quenching effect Effects 0.000 description 1
• 150000003254 radicals Chemical class 0.000 description 1
• 239000011535 reaction buffer Substances 0.000 description 1
• 238000010188 recombinant method Methods 0.000 description 1
• 230000009467 reduction Effects 0.000 description 1
• 230000009712 regulation of translation Effects 0.000 description 1
• 230000010076 replication Effects 0.000 description 1
• 238000011160 research Methods 0.000 description 1
• 238000004007 reversed phase HPLC Methods 0.000 description 1
• 238000012552 review Methods 0.000 description 1
• PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
• 108010066533 ribonuclease S Proteins 0.000 description 1
• 102200141517 rs104893792 Human genes 0.000 description 1
• 102220229765 rs1064794750 Human genes 0.000 description 1
• 102200076676 rs116262672 Human genes 0.000 description 1
• 102220017514 rs118203355 Human genes 0.000 description 1
• 102200061297 rs121909233 Human genes 0.000 description 1
• 102220311297 rs1260392202 Human genes 0.000 description 1
• 102200080930 rs137852221 Human genes 0.000 description 1
• 102200098368 rs137852607 Human genes 0.000 description 1
• 102200139521 rs1419548558 Human genes 0.000 description 1
• 102220072505 rs149348427 Human genes 0.000 description 1
• 102220259360 rs1554037057 Human genes 0.000 description 1
• 102220327268 rs1555509761 Human genes 0.000 description 1
• 102200025794 rs179363887 Human genes 0.000 description 1
• 102200097242 rs199473417 Human genes 0.000 description 1
• 102220108973 rs28763904 Human genes 0.000 description 1
• 102200082883 rs33959855 Human genes 0.000 description 1
• 102220005170 rs33974325 Human genes 0.000 description 1
• 102220108975 rs35196441 Human genes 0.000 description 1
• 102200082880 rs35890959 Human genes 0.000 description 1
• 102220059647 rs370499060 Human genes 0.000 description 1
• 102200021785 rs387906728 Human genes 0.000 description 1
• 102200036809 rs387907341 Human genes 0.000 description 1
• 102220011012 rs397507504 Human genes 0.000 description 1
• 102200054149 rs397515527 Human genes 0.000 description 1
• 102200152570 rs483352923 Human genes 0.000 description 1
• 102200033030 rs587777513 Human genes 0.000 description 1
• 102220045232 rs587781939 Human genes 0.000 description 1
• 102220050124 rs606231308 Human genes 0.000 description 1
• 102200014835 rs727505186 Human genes 0.000 description 1
• 102220113319 rs75087725 Human genes 0.000 description 1
• 102220216944 rs767609311 Human genes 0.000 description 1
• 102220326975 rs770967343 Human genes 0.000 description 1
• 102200072273 rs794726711 Human genes 0.000 description 1
• 102220092414 rs876658033 Human genes 0.000 description 1
• 238000005070 sampling Methods 0.000 description 1
• 238000003118 sandwich ELISA Methods 0.000 description 1
• 230000035945 sensitivity Effects 0.000 description 1
• 238000012163 sequencing technique Methods 0.000 description 1
• 239000002924 silencing RNA Substances 0.000 description 1
• 230000037432 silent mutation Effects 0.000 description 1
• 239000004055 small Interfering RNA Substances 0.000 description 1
• 150000003384 small molecules Chemical class 0.000 description 1
• 229910052708 sodium Inorganic materials 0.000 description 1
• URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
• 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
• 235000017557 sodium bicarbonate Nutrition 0.000 description 1
• QDRKDTQENPPHOJ-UHFFFAOYSA-N sodium ethoxide Chemical compound [Na+].CC[O-] QDRKDTQENPPHOJ-UHFFFAOYSA-N 0.000 description 1
• 229940079827 sodium hydrogen sulfite Drugs 0.000 description 1
• WDCARDDLMCHULC-UHFFFAOYSA-M sodium;2-oxohexanoate Chemical compound [Na+].CCCCC(=O)C([O-])=O WDCARDDLMCHULC-UHFFFAOYSA-M 0.000 description 1
• 239000007790 solid phase Substances 0.000 description 1
• 239000003381 stabilizer Substances 0.000 description 1
• 238000010186 staining Methods 0.000 description 1
• 230000000638 stimulation Effects 0.000 description 1
• 238000012916 structural analysis Methods 0.000 description 1
• 210000001768 subcellular fraction Anatomy 0.000 description 1
• 239000006228 supernatant Substances 0.000 description 1
• 239000006188 syrup Substances 0.000 description 1
• 235000020357 syrup Nutrition 0.000 description 1
• 235000015523 tannic acid Nutrition 0.000 description 1
• 229920002258 tannic acid Polymers 0.000 description 1
• LRBQNJMCXXYXIU-NRMVVENXSA-N tannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-NRMVVENXSA-N 0.000 description 1
• 229940033123 tannic acid Drugs 0.000 description 1
• 239000004250 tert-Butylhydroquinone Substances 0.000 description 1
• FGTJJHCZWOVVNH-UHFFFAOYSA-N tert-butyl-[tert-butyl(dimethyl)silyl]oxy-dimethylsilane Chemical compound CC(C)(C)[Si](C)(C)O[Si](C)(C)C(C)(C)C FGTJJHCZWOVVNH-UHFFFAOYSA-N 0.000 description 1
• 235000019281 tert-butylhydroquinone Nutrition 0.000 description 1
• WROMPOXWARCANT-UHFFFAOYSA-N tfa trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F.OC(=O)C(F)(F)F WROMPOXWARCANT-UHFFFAOYSA-N 0.000 description 1
• 238000002076 thermal analysis method Methods 0.000 description 1
• 125000001544 thienyl group Chemical group 0.000 description 1
• 238000004809 thin layer chromatography Methods 0.000 description 1
• 150000003568 thioethers Chemical class 0.000 description 1
• 125000003396 thiol group Chemical group [H]S* 0.000 description 1
• 229910052719 titanium Inorganic materials 0.000 description 1
• XJDNKRIXUMDJCW-UHFFFAOYSA-J titanium tetrachloride Chemical compound Cl[Ti](Cl)(Cl)Cl XJDNKRIXUMDJCW-UHFFFAOYSA-J 0.000 description 1
• 230000009466 transformation Effects 0.000 description 1
• 230000010474 transient expression Effects 0.000 description 1
• 238000003146 transient transfection Methods 0.000 description 1
• 238000013519 translation Methods 0.000 description 1
• 150000004684 trihydrates Chemical class 0.000 description 1
• 125000000026 trimethylsilyl group Chemical group [H]C([H])([H])[Si]([*])(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
• 229960000281 trometamol Drugs 0.000 description 1
• 229910052721 tungsten Inorganic materials 0.000 description 1
• 241001515965 unidentified phage Species 0.000 description 1
• 210000002700 urine Anatomy 0.000 description 1
• 229960005486 vaccine Drugs 0.000 description 1
• 125000002987 valine group Chemical group [H]N([H])C([H])(C(*)=O)C([H])(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
• 230000007332 vesicle formation Effects 0.000 description 1
• 230000035899 viability Effects 0.000 description 1
• 229920002554 vinyl polymer Polymers 0.000 description 1
• 239000013603 viral vector Substances 0.000 description 1
• 238000011179 visual inspection Methods 0.000 description 1
• 238000010792 warming Methods 0.000 description 1
• 239000003643 water by type Substances 0.000 description 1
• 229910052727 yttrium Inorganic materials 0.000 description 1