RU2012130940A - Дифференцировка человеческих эмбриональных стволовых клеток - Google Patents
Дифференцировка человеческих эмбриональных стволовых клеток Download PDFInfo
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- 230000004069 differentiation Effects 0.000 title 1
- 210000000130 stem cell Anatomy 0.000 title 1
- 210000001900 endoderm Anatomy 0.000 claims abstract 12
- 210000004027 cell Anatomy 0.000 claims abstract 11
- QMGUOJYZJKLOLH-UHFFFAOYSA-N 3-[1-[3-(dimethylamino)propyl]indol-3-yl]-4-(1h-indol-3-yl)pyrrole-2,5-dione Chemical compound C12=CC=CC=C2N(CCCN(C)C)C=C1C1=C(C=2C3=CC=CC=C3NC=2)C(=O)NC1=O QMGUOJYZJKLOLH-UHFFFAOYSA-N 0.000 claims abstract 4
- AMJJLDJPDLKNJA-UHFFFAOYSA-N [hydroxy(naphthalen-2-yl)methyl]phosphonic acid Chemical compound C1=CC=CC2=CC(C(O)P(O)(O)=O)=CC=C21 AMJJLDJPDLKNJA-UHFFFAOYSA-N 0.000 claims abstract 4
- 150000001875 compounds Chemical class 0.000 claims abstract 4
- 238000000034 method Methods 0.000 claims abstract 4
- 210000001778 pluripotent stem cell Anatomy 0.000 claims abstract 4
- 230000002124 endocrine Effects 0.000 claims abstract 3
- QDLHCMPXEPAAMD-QAIWCSMKSA-N wortmannin Chemical compound C1([C@]2(C)C3=C(C4=O)OC=C3C(=O)O[C@@H]2COC)=C4[C@@H]2CCC(=O)[C@@]2(C)C[C@H]1OC(C)=O QDLHCMPXEPAAMD-QAIWCSMKSA-N 0.000 claims abstract 3
- QDLHCMPXEPAAMD-UHFFFAOYSA-N wortmannin Natural products COCC1OC(=O)C2=COC(C3=O)=C2C1(C)C1=C3C2CCC(=O)C2(C)CC1OC(C)=O QDLHCMPXEPAAMD-UHFFFAOYSA-N 0.000 claims abstract 3
- 102100028096 Homeobox protein Nkx-6.2 Human genes 0.000 claims abstract 2
- 101000578254 Homo sapiens Homeobox protein Nkx-6.1 Proteins 0.000 claims abstract 2
- 101000578258 Homo sapiens Homeobox protein Nkx-6.2 Proteins 0.000 claims abstract 2
- 101000603702 Homo sapiens Neurogenin-3 Proteins 0.000 claims abstract 2
- 102100038553 Neurogenin-3 Human genes 0.000 claims abstract 2
- 210000000496 pancreas Anatomy 0.000 claims abstract 2
- 238000012258 culturing Methods 0.000 claims 1
- 230000009996 pancreatic endocrine effect Effects 0.000 claims 1
- 230000024245 cell differentiation Effects 0.000 abstract 1
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Abstract
1. Способ увеличения экспрессии NGN3 и NKX6.1 в популяции клеток, экспрессирующих маркеры, характерные для эндокринной линии поджелудочной железы, включающий следующие стадии:a) культивирования плюрипотентных стволовых клеток,b) дифференцировки плюрипотентных стволовых клеток в клетки, экспрессирующие маркеры, характерные для линии дефинитивной эндодермы,c) дифференцировки клеток, экспрессирующих маркеры, характерные для линии дефинитивной эндодермы, в клетки, экспрессирующие маркеры, характерные для линии панкреатической эндодермы, путем добавления в среду, используемую для дифференцировки линии дефинитивной эндодермы, соединений, выбираемых из группы, которая состоит из H-9, H-89, GF 109203X, HA-1004, PP2, PP1, LY 294002, Вортманнина, SB-203580, SB-202190, Тирфостина 25, Тирфостина, AG1478, Тирфостина 46, GW 5074, Кенпауллона, HNMPA, AG490, Y27632 и ML-7, иd) дифференцировки клеток, экспрессирующих маркеры, характерные для линии панкреатической эндодермы, в клетки, экспресмирующие маркеры, характерные для эндокринной линии поджелудочной железы.2. Способ по п.1, где к среде, используемой для дифференцировки линии панкреатической эндодермы, добавляют соединения, выбираемые из группы, которая состоит из H-9, H-89, GF 109203X, HA-1004, PP2, PP1, LY 294002, Вортманнина, SB-203580, SB-202190, Тирфостина 25, Тирфостина, AG1478, Тирфостина 46, GW 5074, Кенпауллона, HNMPA, AG490, Y27632 и ML-7.
Claims (2)
1. Способ увеличения экспрессии NGN3 и NKX6.1 в популяции клеток, экспрессирующих маркеры, характерные для эндокринной линии поджелудочной железы, включающий следующие стадии:
a) культивирования плюрипотентных стволовых клеток,
b) дифференцировки плюрипотентных стволовых клеток в клетки, экспрессирующие маркеры, характерные для линии дефинитивной эндодермы,
c) дифференцировки клеток, экспрессирующих маркеры, характерные для линии дефинитивной эндодермы, в клетки, экспрессирующие маркеры, характерные для линии панкреатической эндодермы, путем добавления в среду, используемую для дифференцировки линии дефинитивной эндодермы, соединений, выбираемых из группы, которая состоит из H-9, H-89, GF 109203X, HA-1004, PP2, PP1, LY 294002, Вортманнина, SB-203580, SB-202190, Тирфостина 25, Тирфостина, AG1478, Тирфостина 46, GW 5074, Кенпауллона, HNMPA, AG490, Y27632 и ML-7, и
d) дифференцировки клеток, экспрессирующих маркеры, характерные для линии панкреатической эндодермы, в клетки, экспресмирующие маркеры, характерные для эндокринной линии поджелудочной железы.
2. Способ по п.1, где к среде, используемой для дифференцировки линии панкреатической эндодермы, добавляют соединения, выбираемые из группы, которая состоит из H-9, H-89, GF 109203X, HA-1004, PP2, PP1, LY 294002, Вортманнина, SB-203580, SB-202190, Тирфостина 25, Тирфостина, AG1478, Тирфостина 46, GW 5074, Кенпауллона, HNMPA, AG490, Y27632 и ML-7.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US28969209P | 2009-12-23 | 2009-12-23 | |
| US61/289,692 | 2009-12-23 | ||
| PCT/US2010/060770 WO2011079018A2 (en) | 2009-12-23 | 2010-12-16 | Differentiation of human embryonic stem cells |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2016116258A Division RU2664864C1 (ru) | 2009-12-23 | 2010-12-16 | Способы увеличения экспрессии ngn3 и nkx6.1 в эндокринных клетках поджелудочной железы |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2012130940A true RU2012130940A (ru) | 2014-01-27 |
| RU2586506C2 RU2586506C2 (ru) | 2016-06-10 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2012130940/10A RU2586506C2 (ru) | 2009-12-23 | 2010-12-16 | Дифференцировка человеческих эмбриональных стволовых клеток |
| RU2016116258A RU2664864C1 (ru) | 2009-12-23 | 2010-12-16 | Способы увеличения экспрессии ngn3 и nkx6.1 в эндокринных клетках поджелудочной железы |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2016116258A RU2664864C1 (ru) | 2009-12-23 | 2010-12-16 | Способы увеличения экспрессии ngn3 и nkx6.1 в эндокринных клетках поджелудочной железы |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US9133439B2 (ru) |
| EP (1) | EP2516626B1 (ru) |
| JP (1) | JP5882226B2 (ru) |
| KR (3) | KR101764404B1 (ru) |
| CN (1) | CN102712902B (ru) |
| AU (1) | AU2010333840B2 (ru) |
| BR (1) | BR112012015727A2 (ru) |
| CA (1) | CA2784425A1 (ru) |
| ES (1) | ES2633648T3 (ru) |
| MX (1) | MX339669B (ru) |
| PL (1) | PL2516626T3 (ru) |
| RU (2) | RU2586506C2 (ru) |
| SG (2) | SG181685A1 (ru) |
| WO (1) | WO2011079018A2 (ru) |
| ZA (1) | ZA201205488B (ru) |
Families Citing this family (50)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9080145B2 (en) | 2007-07-01 | 2015-07-14 | Lifescan Corporation | Single pluripotent stem cell culture |
| KR101617243B1 (ko) | 2007-07-31 | 2016-05-02 | 라이프스캔, 인코포레이티드 | 인간 배아 줄기 세포의 분화 |
| CN102046779A (zh) | 2008-02-21 | 2011-05-04 | 森托科尔奥索生物科技公司 | 用于细胞粘附、培养和分离的方法、表面改性培养板和组合物 |
| PL2310492T3 (pl) | 2008-06-30 | 2015-12-31 | Janssen Biotech Inc | Różnocowanie pluripotencjalnych komórek macierzystych |
| AU2009316583B2 (en) | 2008-11-20 | 2016-04-21 | Janssen Biotech, Inc. | Methods and compositions for cell attachment and cultivation on planar substrates |
| MX356756B (es) | 2008-11-20 | 2018-06-11 | Centocor Ortho Biotech Inc | Células madre pluripotentes en microportadores. |
| EP2456862A4 (en) | 2009-07-20 | 2013-02-27 | Janssen Biotech Inc | DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS |
| CN102741395B (zh) | 2009-12-23 | 2016-03-16 | 詹森生物科技公司 | 人胚胎干细胞的分化 |
| RU2702198C2 (ru) | 2010-03-01 | 2019-10-04 | Янссен Байотек, Инк. | Способы очистки клеток, производных от плюрипотентных стволовых клеток |
| WO2011140441A2 (en) | 2010-05-06 | 2011-11-10 | Children's Hospital Medical Center | Methods and systems for converting precursor cells into intestinal tissues through directed differentiation |
| KR101851956B1 (ko) | 2010-08-31 | 2018-04-25 | 얀센 바이오테크 인코포레이티드 | 인간 배아 줄기 세포의 분화 |
| US20140234963A1 (en) | 2011-06-21 | 2014-08-21 | Novo Nordisk A/S | Efficient induction of definitive endoderm from pluripotent stem cells |
| KR102203056B1 (ko) | 2011-12-22 | 2021-01-14 | 얀센 바이오테크 인코포레이티드 | 인간 배아 줄기 세포의 단일 인슐린 호르몬 양성 세포로의 분화 |
| CN108034633B (zh) | 2012-06-08 | 2022-08-02 | 詹森生物科技公司 | 人胚胎干细胞向胰腺内分泌细胞的分化 |
| US20150247123A1 (en) | 2012-09-03 | 2015-09-03 | Novo Nordisk A/S | Generation of pancreatic endoderm from Pluripotent Stem cells using small molecules |
| USD933584S1 (en) | 2012-11-08 | 2021-10-19 | Sunpower Corporation | Solar panel |
| USD1009775S1 (en) | 2014-10-15 | 2024-01-02 | Maxeon Solar Pte. Ltd. | Solar panel |
| US10876096B2 (en) | 2012-11-28 | 2020-12-29 | Corning Incorporated | Cell culture medium for enhanced hepatocyte function |
| AU2013368224B2 (en) | 2012-12-31 | 2018-09-27 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells into pancreatic endocrine cells using HB9 regulators |
| BR112015015714A2 (pt) * | 2012-12-31 | 2017-07-11 | Janssen Biotech Inc | suspensão e aglomeração de células pluripotentes humanas para diferenciação em célu-las endócrinas pancreáticas |
| EP2938724B1 (en) | 2012-12-31 | 2020-10-28 | Janssen Biotech, Inc. | Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells |
| US10370644B2 (en) | 2012-12-31 | 2019-08-06 | Janssen Biotech, Inc. | Method for making human pluripotent suspension cultures and cells derived therefrom |
| US8859286B2 (en) | 2013-03-14 | 2014-10-14 | Viacyte, Inc. | In vitro differentiation of pluripotent stem cells to pancreatic endoderm cells (PEC) and endocrine cells |
| US10081609B2 (en) | 2013-03-15 | 2018-09-25 | The Broad Institute, Inc. | Compounds for inducing proliferation and differentiation of cells, and methods of use thereof |
| EP3143127B1 (en) * | 2014-05-16 | 2021-07-14 | Janssen Biotech, Inc. | Use of small molecules to enhance mafa expression in pancreatic endocrine cells |
| WO2015183920A2 (en) | 2014-05-28 | 2015-12-03 | Children's Hospital Medical Center | Methods and systems for converting precursor cells into gastric tissues through directed differentiation |
| USD913210S1 (en) | 2014-10-15 | 2021-03-16 | Sunpower Corporation | Solar panel |
| USD933585S1 (en) | 2014-10-15 | 2021-10-19 | Sunpower Corporation | Solar panel |
| USD999723S1 (en) | 2014-10-15 | 2023-09-26 | Sunpower Corporation | Solar panel |
| USD896747S1 (en) | 2014-10-15 | 2020-09-22 | Sunpower Corporation | Solar panel |
| WO2016061464A1 (en) | 2014-10-17 | 2016-04-21 | Children's Hospital Center, D/B/A Cincinnati Children's Hospital Medical Center | In vivo model of human small intetine using pluripotent stem cells and methods of making and using same |
| JPWO2016104541A1 (ja) * | 2014-12-24 | 2017-10-05 | 国立大学法人京都大学 | 内胚葉系細胞の製造方法、肝臓細胞の製造方法、膵臓細胞の製造方法、内胚葉系細胞の誘導促進剤、肝臓細胞の誘導促進キット、膵臓細胞の誘導促進キット、およびマイクロ流体デバイス |
| MA45479A (fr) | 2016-04-14 | 2019-02-20 | Janssen Biotech Inc | Différenciation de cellules souches pluripotentes en cellules de l'endoderme de l'intestin moyen |
| CN116790476A (zh) | 2016-05-05 | 2023-09-22 | 儿童医院医疗中心 | 用于体外制造胃底组织的方法和与其相关的组合物 |
| WO2018106628A1 (en) | 2016-12-05 | 2018-06-14 | Children's Hospital Medical Center | Colonic organoids and methods of making and using same |
| US10767164B2 (en) | 2017-03-30 | 2020-09-08 | The Research Foundation For The State University Of New York | Microenvironments for self-assembly of islet organoids from stem cells differentiation |
| US10391156B2 (en) | 2017-07-12 | 2019-08-27 | Viacyte, Inc. | University donor cells and related methods |
| KR102244910B1 (ko) | 2018-03-16 | 2021-04-26 | 주식회사 엘지화학 | 세리아-탄소-황 복합체, 이의 제조방법, 이를 포함하는 양극 및 리튬-황 전지 |
| EP3801638A1 (en) * | 2018-06-08 | 2021-04-14 | Novartis AG | Cell-based assay for measuring drug product potency |
| US10724052B2 (en) | 2018-09-07 | 2020-07-28 | Crispr Therapeutics Ag | Universal donor cells |
| CA3139590C (en) | 2019-05-31 | 2024-01-23 | W. L. Gore & Associates, Inc. | A biocompatible membrane composite |
| CN114401752B (zh) | 2019-05-31 | 2023-04-04 | W.L.戈尔及同仁股份有限公司 | 具有受控氧扩散距离的细胞封装装置 |
| EP3975926A1 (en) | 2019-05-31 | 2022-04-06 | W.L. Gore & Associates, Inc. | A biocompatible membrane composite |
| AU2020282355B2 (en) | 2019-05-31 | 2023-11-02 | Viacyte, Inc. | A biocompatible membrane composite |
| KR20220058579A (ko) | 2019-09-05 | 2022-05-09 | 크리스퍼 테라퓨틱스 아게 | 보편적 공여자 세포 |
| CA3150235A1 (en) | 2019-09-05 | 2021-03-11 | Alireza Rezania | UNIVERSAL DONOR CELLS |
| CN111057677B (zh) * | 2019-12-27 | 2021-08-03 | 浙江大学 | 一种软骨祖细胞培养基及其制备方法和应用 |
| AU2021414617A1 (en) | 2020-12-31 | 2023-08-10 | Crispr Therapeutics Ag | Universal donor cells |
| EP4330376A1 (en) * | 2021-04-28 | 2024-03-06 | Shandong University | Methods for improving early embryo development |
| CN113234664B (zh) * | 2021-05-11 | 2024-05-10 | 澳门大学 | 一种胰腺祖细胞的制备方法及其应用 |
Family Cites Families (188)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3209652A (en) | 1961-03-30 | 1965-10-05 | Burgsmueller Karl | Thread whirling method |
| AT326803B (de) | 1968-08-26 | 1975-12-29 | Binder Fa G | Maschenware sowie verfahren zur herstellung derselben |
| US3935067A (en) * | 1974-11-22 | 1976-01-27 | Wyo-Ben Products, Inc. | Inorganic support for culture media |
| CA1201400A (en) | 1982-04-16 | 1986-03-04 | Joel L. Williams | Chemically specific surfaces for influencing cell activity during culture |
| US4499802A (en) | 1982-09-29 | 1985-02-19 | Container Graphics Corporation | Rotary cutting die with scrap ejection |
| US4537773A (en) | 1983-12-05 | 1985-08-27 | E. I. Du Pont De Nemours And Company | α-Aminoboronic acid derivatives |
| US4557264A (en) | 1984-04-09 | 1985-12-10 | Ethicon Inc. | Surgical filament from polypropylene blended with polyethylene |
| US5215893A (en) * | 1985-10-03 | 1993-06-01 | Genentech, Inc. | Nucleic acid encoding the ba chain prodomains of inhibin and method for synthesizing polypeptides using such nucleic acid |
| US5089396A (en) * | 1985-10-03 | 1992-02-18 | Genentech, Inc. | Nucleic acid encoding β chain prodomains of inhibin and method for synthesizing polypeptides using such nucleic acid |
| US4737578A (en) * | 1986-02-10 | 1988-04-12 | The Salk Institute For Biological Studies | Human inhibin |
| US5863531A (en) * | 1986-04-18 | 1999-01-26 | Advanced Tissue Sciences, Inc. | In vitro preparation of tubular tissue structures by stromal cell culture on a three-dimensional framework |
| CA1340581C (en) * | 1986-11-20 | 1999-06-08 | Joseph P. Vacanti | Chimeric neomorphogenesis of organs by controlled cellular implantation using artificial matrices |
| US5759830A (en) * | 1986-11-20 | 1998-06-02 | Massachusetts Institute Of Technology | Three-dimensional fibrous scaffold containing attached cells for producing vascularized tissue in vivo |
| US5567612A (en) * | 1986-11-20 | 1996-10-22 | Massachusetts Institute Of Technology | Genitourinary cell-matrix structure for implantation into a human and a method of making |
| NZ229354A (en) | 1988-07-01 | 1990-09-26 | Becton Dickinson Co | Treating polymer surfaces with a gas plasma and then applying a layer of endothelial cells to the surface |
| EP0363125A3 (en) | 1988-10-03 | 1990-08-16 | Hana Biologics Inc. | Proliferated pancreatic endocrine cell product and process |
| SU1767433A1 (ru) * | 1989-11-27 | 1992-10-07 | Пермский государственный медицинский институт | Способ определени инсулинорезистентности имунного генеза у больных сахарным диабетом I типа |
| US5837539A (en) * | 1990-11-16 | 1998-11-17 | Osiris Therapeutics, Inc. | Monoclonal antibodies for human mesenchymal stem cells |
| DK0628639T3 (da) | 1991-04-25 | 2000-01-24 | Chugai Pharmaceutical Co Ltd | Rekonstitueret humant antistof mod human interleukin-6-receptor |
| US5449383A (en) * | 1992-03-18 | 1995-09-12 | Chatelier; Ronald C. | Cell growth substrates |
| GB9206861D0 (en) | 1992-03-28 | 1992-05-13 | Univ Manchester | Wound healing and treatment of fibrotic disorders |
| CA2114282A1 (en) | 1993-01-28 | 1994-07-29 | Lothar Schilder | Multi-layered implant |
| JP3525221B2 (ja) | 1993-02-17 | 2004-05-10 | 味の素株式会社 | 免疫抑制剤 |
| US5523226A (en) * | 1993-05-14 | 1996-06-04 | Biotechnology Research And Development Corp. | Transgenic swine compositions and methods |
| GB9310557D0 (en) * | 1993-05-21 | 1993-07-07 | Smithkline Beecham Plc | Novel process and apparatus |
| TW257671B (ru) | 1993-11-19 | 1995-09-21 | Ciba Geigy | |
| US6703017B1 (en) * | 1994-04-28 | 2004-03-09 | Ixion Biotechnology, Inc. | Reversal of insulin-dependent diabetes by islet-producing stem cells, islet progenitor cells and islet-like structures |
| US5834308A (en) * | 1994-04-28 | 1998-11-10 | University Of Florida Research Foundation, Inc. | In vitro growth of functional islets of Langerhans |
| US6001647A (en) | 1994-04-28 | 1999-12-14 | Ixion Biotechnology, Inc. | In vitro growth of functional islets of Langerhans and in vivo uses thereof |
| US6083903A (en) | 1994-10-28 | 2000-07-04 | Leukosite, Inc. | Boronic ester and acid compounds, synthesis and uses |
| JP4079461B2 (ja) | 1994-12-29 | 2008-04-23 | 中外製薬株式会社 | Ｉｌ−６アンタゴニストを含んでなる抗腫瘍剤の作用増強剤 |
| US5843780A (en) * | 1995-01-20 | 1998-12-01 | Wisconsin Alumni Research Foundation | Primate embryonic stem cells |
| US5718922A (en) * | 1995-05-31 | 1998-02-17 | Schepens Eye Research Institute, Inc. | Intravitreal microsphere drug delivery and method of preparation |
| US5908782A (en) * | 1995-06-05 | 1999-06-01 | Osiris Therapeutics, Inc. | Chemically defined medium for human mesenchymal stem cells |
| UA65572C2 (en) | 1997-04-24 | 2004-04-15 | Ortho Mcneil Pharm Inc | Substituted imidazoles, intermediate compounds for the preparation thereof, a method for the preparation of substituted imidazoles and a method for the treatment of inflammatory diseases |
| DK1028737T3 (da) * | 1997-07-03 | 2007-08-13 | Osiris Therapeutics Inc | Humane mesenchymale stamceller fra perifert blod |
| WO1999014318A1 (en) * | 1997-09-16 | 1999-03-25 | Board Of Regents, The University Of Texas System | Method for the complete chemical synthesis and assembly of genes and genomes |
| US6670127B2 (en) | 1997-09-16 | 2003-12-30 | Egea Biosciences, Inc. | Method for assembly of a polynucleotide encoding a target polypeptide |
| JP3880795B2 (ja) | 1997-10-23 | 2007-02-14 | ジェロン・コーポレーション | フィーダー細胞を含まない培養物中で、霊長類由来始原幹細胞を増殖させるための方法 |
| AR014195A1 (es) * | 1997-12-29 | 2001-02-07 | Ortho Mcneil Pharm Inc | Compuestos de trifenilpropanamida utiles para el tratamiento de procesos inflamatorios, composiciones anti-inflamatorias que los comprenden, ymetodos para prepararlos |
| US6328960B1 (en) | 1998-03-18 | 2001-12-11 | Osiris Therapeutics, Inc. | Mesenchymal stem cells for prevention and treatment of immune responses in transplantation |
| MY132496A (en) * | 1998-05-11 | 2007-10-31 | Vertex Pharma | Inhibitors of p38 |
| US6413773B1 (en) * | 1998-06-01 | 2002-07-02 | The Regents Of The University Of California | Phosphatidylinositol 3-kinase inhibitors as stimulators of endocrine differentiation |
| US7410798B2 (en) | 2001-01-10 | 2008-08-12 | Geron Corporation | Culture system for rapid expansion of human embryonic stem cells |
| US6667176B1 (en) | 2000-01-11 | 2003-12-23 | Geron Corporation | cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells |
| US6610540B1 (en) | 1998-11-18 | 2003-08-26 | California Institute Of Technology | Low oxygen culturing of central nervous system progenitor cells |
| US6413556B1 (en) * | 1999-01-08 | 2002-07-02 | Sky High, Llc | Aqueous anti-apoptotic compositions |
| EP1144597A2 (en) * | 1999-01-21 | 2001-10-17 | Vitro Diagnostics, Inc. | Immortalized cell lines and methods of making the same |
| US6815203B1 (en) * | 1999-06-23 | 2004-11-09 | Joslin Diabetes Center, Inc. | Methods of making pancreatic islet cells |
| US6333029B1 (en) * | 1999-06-30 | 2001-12-25 | Ethicon, Inc. | Porous tissue scaffoldings for the repair of regeneration of tissue |
| US6306424B1 (en) * | 1999-06-30 | 2001-10-23 | Ethicon, Inc. | Foam composite for the repair or regeneration of tissue |
| US6685936B2 (en) * | 1999-10-12 | 2004-02-03 | Osiris Therapeutics, Inc. | Suppressor cells induced by culture with mesenchymal stem cells for treatment of immune responses in transplantation |
| US20030082155A1 (en) | 1999-12-06 | 2003-05-01 | Habener Joel F. | Stem cells of the islets of langerhans and their use in treating diabetes mellitus |
| US6753153B2 (en) | 1999-12-13 | 2004-06-22 | The Scripps Research Institute | Markers for identification and isolation of pancreatic islet α and β progenitors |
| US7005252B1 (en) * | 2000-03-09 | 2006-02-28 | Wisconsin Alumni Research Foundation | Serum free cultivation of primate embryonic stem cells |
| US7439064B2 (en) * | 2000-03-09 | 2008-10-21 | Wicell Research Institute, Inc. | Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium |
| US6436704B1 (en) * | 2000-04-10 | 2002-08-20 | Raven Biotechnologies, Inc. | Human pancreatic epithelial progenitor cells and methods of isolation and use thereof |
| US6458589B1 (en) * | 2000-04-27 | 2002-10-01 | Geron Corporation | Hepatocyte lineage cells derived from pluripotent stem cells |
| KR100947577B1 (ko) * | 2000-06-26 | 2010-03-15 | 엔씨 메디컬 리서치 가부시키가이샤 | 신경계 세포로 분화할 수 있는 세포를 포함하는 세포분획 |
| DK1333833T3 (da) * | 2000-10-23 | 2011-12-12 | Glaxosmithkline Llc | Ny trisubstitueret 8H-pyridol[2,3-d]pyrimidin-7-on-forbindelse til behandling af CSBP/RK/p38-kinnasemedierede sygdomme |
| AU2737102A (en) | 2000-12-08 | 2002-06-18 | Ortho Mcneil Pharm Inc | Macroheterocylic compounds useful as kinase inhibitors |
| ES2263681T3 (es) | 2000-12-08 | 2006-12-16 | Ortho-Mcneil Pharmaceutical, Inc. | Compuestos de pirrolina indazolil-substituidos como inhibidores de la kinasa. |
| US6599323B2 (en) * | 2000-12-21 | 2003-07-29 | Ethicon, Inc. | Reinforced tissue implants and methods of manufacture and use |
| CA2435826A1 (en) | 2001-01-24 | 2002-08-01 | The Government Of The United States Of America | Differentiation of stem cells to pancreatic endocrine cells |
| CA2435124A1 (en) | 2001-01-25 | 2002-08-01 | Millennium Pharmaceuticals, Inc. | Formulation of boronic acid compounds |
| US6656488B2 (en) | 2001-04-11 | 2003-12-02 | Ethicon Endo-Surgery, Inc. | Bioabsorbable bag containing bioabsorbable materials of different bioabsorption rates for tissue engineering |
| EP1379626A2 (en) * | 2001-04-19 | 2004-01-14 | DeveloGen Aktiengesellschaft für entwicklungsbiologische Forschung | A method for differentiating stem cells into insulin-producing cells |
| WO2002088335A1 (fr) | 2001-04-24 | 2002-11-07 | Ajinomoto Co., Inc. | Cellules souches et procede d'extraction de ces cellules |
| CA2447015A1 (en) | 2001-05-15 | 2002-11-21 | Rappaport Family Institute For Research In The Medical Sciences | Insulin producing cells derived from human embryonic stem cells |
| US6626950B2 (en) * | 2001-06-28 | 2003-09-30 | Ethicon, Inc. | Composite scaffold with post anchor for the repair and regeneration of tissue |
| KR100418195B1 (ko) | 2001-07-05 | 2004-02-11 | 주식회사 우리기술 | 전력케이블의 다중절연진단장치 및 그 방법 |
| GB0117583D0 (en) * | 2001-07-19 | 2001-09-12 | Astrazeneca Ab | Novel compounds |
| WO2003014313A2 (en) * | 2001-08-06 | 2003-02-20 | Bresagen, Ltd. | Alternative compositions and methods for the culture of stem cells |
| US6617152B2 (en) * | 2001-09-04 | 2003-09-09 | Corning Inc | Method for creating a cell growth surface on a polymeric substrate |
| EP1298201A1 (en) * | 2001-09-27 | 2003-04-02 | Cardion AG | Process for the production of cells exhibiting an islet-beta-cell-like state |
| US20030138951A1 (en) * | 2001-10-18 | 2003-07-24 | Li Yin | Conversion of liver stem and progenitor cells to pancreatic functional cells |
| JP4330995B2 (ja) | 2001-11-15 | 2009-09-16 | チルドレンズ メディカル センター コーポレーション | 絨毛膜絨毛、羊水、および胎盤からの胎児性幹細胞を単離、増殖、および分化させる方法、ならびにその治療的使用方法 |
| KR100930139B1 (ko) | 2001-12-07 | 2009-12-07 | 사이토리 테라퓨틱스, 인크. | 처리된 리포애스퍼레이트 세포로 환자를 치료하기 위한시스템 및 방법 |
| WO2003050249A2 (en) | 2001-12-07 | 2003-06-19 | Geron Corporation | Islet cells from human embryonic stem cells |
| WO2003054169A1 (en) | 2001-12-21 | 2003-07-03 | Thromb-X Nv | Compositions for the in vitro derivation and culture of embryonic stem (es) cell lines with germline transmission capability |
| US20030162290A1 (en) | 2002-01-25 | 2003-08-28 | Kazutomo Inoue | Method for inducing differentiation of embryonic stem cells into functioning cells |
| JPWO2003087349A1 (ja) * | 2002-04-17 | 2005-08-18 | 大塚製薬株式会社 | 間葉系細胞から膵β細胞を形成する方法 |
| US20040161419A1 (en) * | 2002-04-19 | 2004-08-19 | Strom Stephen C. | Placental stem cells and uses thereof |
| EP1506192B1 (en) | 2002-05-08 | 2008-02-27 | Janssen Pharmaceutica N.V. | Substituted pyrroline kinase inhibitors |
| US20060003446A1 (en) * | 2002-05-17 | 2006-01-05 | Gordon Keller | Mesoderm and definitive endoderm cell populations |
| BR0311360A (pt) | 2002-05-28 | 2006-06-06 | Becton Dickinson Co | métodos para expansão e transdiferenciação in vitro de células acinares pancreáticas humanas em células produtoras de insulina |
| JP2005531609A (ja) * | 2002-06-05 | 2005-10-20 | ジヤンセン・フアーマシユーチカ・ナームローゼ・フエンノートシヤツプ | キナーゼ阻害剤としてのシスインドリル−マレイミド誘導体 |
| GB0212976D0 (en) | 2002-06-06 | 2002-07-17 | Tonejet Corp Pty Ltd | Ejection method and apparatus |
| CN1171991C (zh) | 2002-07-08 | 2004-10-20 | 徐如祥 | 人神经干细胞的培养方法 |
| US6877147B2 (en) * | 2002-07-22 | 2005-04-05 | Broadcom Corporation | Technique to assess timing delay by use of layout quality analyzer comparison |
| US7838290B2 (en) * | 2002-07-25 | 2010-11-23 | The Scripps Research Institute | Hematopoietic stem cells and methods of treatment of neovascular eye diseases therewith |
| CA2494040A1 (en) | 2002-07-29 | 2004-02-05 | Es Cell International Pte Ltd. | Multi-step method for the differentiation of insulin positive, glucose |
| AU2003262628A1 (en) | 2002-08-14 | 2004-03-03 | University Of Florida | Bone marrow cell differentiation |
| AU2003268534A1 (en) * | 2002-09-06 | 2004-03-29 | Amcyte Inc. | Cd56 positive human adult pancreatic endocrine progenitor cells |
| US9969977B2 (en) * | 2002-09-20 | 2018-05-15 | Garnet Biotherapeutics | Cell populations which co-express CD49c and CD90 |
| US20040062753A1 (en) * | 2002-09-27 | 2004-04-01 | Alireza Rezania | Composite scaffolds seeded with mammalian cells |
| AU2003285172A1 (en) | 2002-11-08 | 2004-06-03 | The Johns Hopkins University | Human embryonic stem cell cultures, and compositions and methods for growing same |
| US7144999B2 (en) * | 2002-11-23 | 2006-12-05 | Isis Pharmaceuticals, Inc. | Modulation of hypoxia-inducible factor 1 alpha expression |
| WO2004050827A2 (en) | 2002-12-05 | 2004-06-17 | Technion Research & Development Foundation Ltd. | Cultured human pancreatic islets, and uses thereof |
| JP4613069B2 (ja) | 2002-12-16 | 2011-01-12 | テクニオン リサーチ アンド ディベロップメント ファウンデーション リミテッド | 支持細胞非含有、異種非含有のヒト胚性幹細胞の調製方法およびこれらを使用して調製された幹細胞培養物 |
| RU2359671C2 (ru) * | 2003-01-29 | 2009-06-27 | Такеда Фармасьютикал Компани Лимитед | Способ получения препарата с покрытием |
| US20070154981A1 (en) * | 2003-02-14 | 2007-07-05 | The Board Of Trustees Of The Leland Stanford Junior University | Insulin-producing cells derived from stem cells |
| WO2004073633A2 (en) | 2003-02-14 | 2004-09-02 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and compositions for modulating the development of stem cells |
| CA2520861A1 (en) | 2003-03-27 | 2004-10-14 | Ixion Biotechnology, Inc. | Method for transdifferentiation of non-pancreatic stem cells to the pancreatic pathway |
| WO2004090110A2 (en) | 2003-03-31 | 2004-10-21 | Bresagen Inc. | Compositions and methods for the control, differentiation and/or manipulation of pluripotent cells through a gamma-secretase signaling pathway |
| US20090203141A1 (en) * | 2003-05-15 | 2009-08-13 | Shi-Lung Lin | Generation of tumor-free embryonic stem-like pluripotent cells using inducible recombinant RNA agents |
| PL1641914T3 (pl) * | 2003-06-27 | 2017-01-31 | DePuy Synthes Products, Inc. | Komórki pochodzące z poporodowej tkanki łożyska oraz sposoby uzyskiwania i zastosowania tych komórek |
| IL161903A0 (en) * | 2003-07-17 | 2005-11-20 | Gamida Cell Ltd | Ex vivo progenitor and stem cell expansion for usein the treatment of disease of endodermally- deri ved organs |
| ITRM20030395A1 (it) | 2003-08-12 | 2005-02-13 | Istituto Naz Per Le Malattie Infettive Lazz | Terreno di coltura per il mantenimento, la proliferazione e il differenziamento di cellule di mammifero. |
| US20050042595A1 (en) * | 2003-08-14 | 2005-02-24 | Martin Haas | Banking of multipotent amniotic fetal stem cells |
| US7157275B2 (en) * | 2003-08-15 | 2007-01-02 | Becton, Dickinson And Company | Peptides for enhanced cell attachment and growth |
| US20060205072A1 (en) * | 2003-08-27 | 2006-09-14 | Nobuko Uchida | Enriched pancreatic stem cell and progenitor cell populations, and methods for identifying, isolating and enriching for such populations |
| EP1696899A1 (en) * | 2003-12-17 | 2006-09-06 | Allergan, Inc. | Methods for treating retinoid responsive disorders using selective inhibitors of cyp26a and cyp26b |
| US20060030042A1 (en) * | 2003-12-19 | 2006-02-09 | Ali Brivanlou | Maintenance of embryonic stem cells by the GSK-3 inhibitor 6-bromoindirubin-3'-oxime |
| US7625753B2 (en) | 2003-12-23 | 2009-12-01 | Cythera, Inc. | Expansion of definitive endoderm cells |
| US20050266554A1 (en) | 2004-04-27 | 2005-12-01 | D Amour Kevin A | PDX1 expressing endoderm |
| CN103898045B (zh) * | 2003-12-23 | 2019-02-01 | 维亚希特公司 | 定形内胚层 |
| WO2005065354A2 (en) * | 2003-12-31 | 2005-07-21 | The Burnham Institute | Defined media for pluripotent stem cell culture |
| TWI334443B (en) * | 2003-12-31 | 2010-12-11 | Ind Tech Res Inst | Method of single cell culture of undifferentiated human embryonic stem cells |
| WO2005071066A1 (en) * | 2004-01-23 | 2005-08-04 | Board Of Regents, The University Of Texas System | Methods and compositions for preparing pancreatic insulin secreting cells |
| US20070298453A1 (en) | 2004-02-12 | 2007-12-27 | University Of Newcastle Upon Tyne | Stem Cells |
| US20060281174A1 (en) | 2004-03-09 | 2006-12-14 | Gang Xu | Methods for generating insulin-producing cells |
| EP1730261A4 (en) | 2004-03-10 | 2007-11-28 | Univ California | COMPOSITIONS AND METHODS FOR GROWING EMBRYONIC STEM CELLS |
| AU2005230832B2 (en) * | 2004-04-01 | 2010-11-11 | Wisconsin Alumni Research Foundation | Differentiation of stem cells to endoderm and pancreatic lineage |
| DK2377922T3 (da) | 2004-04-27 | 2020-05-04 | Viacyte Inc | PDX1-eksprimerende endoderm |
| CN102925406B (zh) | 2004-07-09 | 2019-11-22 | 维亚希特公司 | 鉴定用于分化定型内胚层的因子的方法 |
| WO2006020919A2 (en) | 2004-08-13 | 2006-02-23 | University Of Georgia Research Foundation, Inc. | Compositions and methods for self-renewal and differentiation in human embryonic stem cells |
| US20080268533A1 (en) * | 2004-08-25 | 2008-10-30 | University Of Georgia Research Foundation, Inc. | Methods and Compositions Utilizing Myc and Gsk3Beta to Manipulate the Pluripotency of Embryonic Stem Cells |
| DE102004043256B4 (de) | 2004-09-07 | 2013-09-19 | Rheinische Friedrich-Wilhelms-Universität Bonn | Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension |
| JP5420837B2 (ja) | 2004-09-08 | 2014-02-19 | ウィスコンシン アラムニ リサーチ ファンデーション | 胚幹細胞の培地及び培養 |
| KR20070058584A (ko) * | 2004-09-08 | 2007-06-08 | 위스콘신 얼럼나이 리서어치 화운데이션 | 인간 배아 줄기 세포의 배양 |
| JP2008528038A (ja) * | 2005-01-31 | 2008-07-31 | エス セル インターナショナル ピーティーイー リミテッド | 胚性幹細胞の指示された分化及びその利用 |
| ES2627419T3 (es) | 2005-03-04 | 2017-07-28 | Lifescan, Inc. | Células estromales adultas derivadas del páncreas |
| GB0505970D0 (en) | 2005-03-23 | 2005-04-27 | Univ Edinburgh | Culture medium containing kinase inhibitor, and uses thereof |
| WO2006113470A2 (en) | 2005-04-15 | 2006-10-26 | Geron Corporation | Cancer treatment by combined inhibition of proteasome and telomerase activities |
| US20080208351A1 (en) | 2005-04-26 | 2008-08-28 | Aarhus Universitet | Biocompatible Material for Surgical Implants and Cell Guiding Tissue Culture Surfaces |
| US20100234400A1 (en) | 2005-06-10 | 2010-09-16 | Irm Llc | Compounds that maintain pluripotency of embryonic stem cells |
| WO2006138433A2 (en) | 2005-06-14 | 2006-12-28 | The Regents Of The University Of California | Induction of cell differentiation by class i bhlh polypeptides |
| EP1931764A1 (en) | 2005-06-21 | 2008-06-18 | GE Healthcare Bio-Sciences AB | Method for cell culture |
| NZ564179A (en) | 2005-06-30 | 2010-09-30 | Janssen Pharmaceutica Nv | Cyclic anilino - pyridinotriazines as GSK-3 inhibitors |
| US20080194021A1 (en) | 2005-07-29 | 2008-08-14 | Mays Robert W | Use of a Gsk-3 Inhibitor to Maintain Potency of Culture Cells |
| US20090087907A1 (en) | 2005-07-29 | 2009-04-02 | Alice Pebay | Compositions and Methods for Growth of Pluripotent Cells |
| KR20080056182A (ko) | 2005-09-02 | 2008-06-20 | 에이전시 포 사이언스, 테크놀로지 앤드 리서치 | 간엽 줄기 세포의 유도 방법 |
| GB2444686B (en) | 2005-09-12 | 2010-08-25 | Es Cell Int Pte Ltd | Differentiation of pluripotent stem cells using p38 MAPK inhibitors or prostaglandins |
| EP1941032A2 (en) | 2005-10-14 | 2008-07-09 | Regents Of The University Of Minnesota | Differentiation of non-embryonic stem cells to cells having a pancreatic phenotype |
| EP1957636B1 (en) | 2005-10-27 | 2018-07-04 | Viacyte, Inc. | Pdx1-expressing dorsal and ventral foregut endoderm |
| WO2007082963A1 (es) | 2006-01-18 | 2007-07-26 | Fundación Instituto Valenciano De Infertilidad | Líneas de células madre embrionarias humanas y métodos para usar las mismas |
| SG170021A1 (en) | 2006-02-23 | 2011-04-29 | Novocell Inc | Compositions and methods useful for culturing differentiable cells |
| US7695965B2 (en) * | 2006-03-02 | 2010-04-13 | Cythera, Inc. | Methods of producing pancreatic hormones |
| EP2650359B1 (en) | 2006-03-02 | 2022-05-04 | Viacyte, Inc. | Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production |
| EP4438720A2 (en) | 2006-04-28 | 2024-10-02 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells |
| US8741643B2 (en) * | 2006-04-28 | 2014-06-03 | Lifescan, Inc. | Differentiation of pluripotent stem cells to definitive endoderm lineage |
| US20070259423A1 (en) | 2006-05-02 | 2007-11-08 | Jon Odorico | Method of differentiating stem cells into cells of the endoderm and pancreatic lineage |
| US7964402B2 (en) | 2006-05-25 | 2011-06-21 | Sanford-Burnham Medical Research Institute | Methods for culture and production of single cell populations of human embryonic stem cells |
| US8415153B2 (en) | 2006-06-19 | 2013-04-09 | Geron Corporation | Differentiation and enrichment of islet-like cells from human pluripotent stem cells |
| CN100494359C (zh) | 2006-06-23 | 2009-06-03 | 中日友好医院 | 神经干细胞三维立体培养体外扩增的方法 |
| US20080003676A1 (en) | 2006-06-26 | 2008-01-03 | Millipore Corporation | Growth of embryonic stem cells |
| WO2008036447A2 (en) | 2006-06-26 | 2008-03-27 | Lifescan, Inc. | Pluripotent stem cell culture |
| WO2008004990A2 (en) | 2006-07-06 | 2008-01-10 | Es Cell International Pte Ltd | Method for stem cell culture and cells derived therefrom |
| WO2008013664A2 (en) | 2006-07-26 | 2008-01-31 | Cythera, Inc. | Methods of producing pancreatic hormones |
| KR101331510B1 (ko) | 2006-08-30 | 2013-11-20 | 재단법인서울대학교산학협력재단 | 저농도의 포도당을 함유하는 인간 배아줄기세포용 배지조성물 및 이를 이용한 인간 배아 줄기세포로부터 인슐린생산 세포 또는 세포괴로 분화시키는 방법, 그리고그로부터 유도된 인슐린 생산 세포 또는 세포괴 |
| JP2008099662A (ja) | 2006-09-22 | 2008-05-01 | Institute Of Physical & Chemical Research | 幹細胞の培養方法 |
| WO2008039521A2 (en) * | 2006-09-26 | 2008-04-03 | Nmt Medical, Inc. | Method for modifying a medical implant surface for promoting tissue growth |
| WO2008048647A1 (en) | 2006-10-17 | 2008-04-24 | Cythera, Inc. | Modulation of the phosphatidylinositol-3-kinase pathway in the differentiation of human embryonic stem cells |
| CA2666789C (en) | 2006-10-18 | 2016-11-22 | Yong Zhao | Embryonic-like stem cells derived from adult human peripheral blood and methods of use |
| WO2008086005A1 (en) | 2007-01-09 | 2008-07-17 | University Of South Florida | Compositions including triciribine and bortezomib and derivatives thereof and methods of use thereof |
| WO2008094597A2 (en) | 2007-01-30 | 2008-08-07 | University Of Georgia Research Foundation, Inc. | Early mesoderm cells, a stable population of mesendoderm cells that has utility for generation of endoderm and mesoderm lineages and multipotent migratory cells (mmc) |
| GB0703188D0 (en) | 2007-02-19 | 2007-03-28 | Roger Land Building | Large scale production of stem cells |
| US7839480B2 (en) * | 2007-04-25 | 2010-11-23 | Taiwan Semiconductor Manufacturing Company, Ltd. | Photomask haze reduction via ventilation |
| BRPI0814425A2 (pt) | 2007-07-18 | 2014-10-21 | Lifescan Inc | Diferenciação de células-tronco embrionárias humanas |
| KR101617243B1 (ko) | 2007-07-31 | 2016-05-02 | 라이프스캔, 인코포레이티드 | 인간 배아 줄기 세포의 분화 |
| KR101544498B1 (ko) | 2007-08-24 | 2015-08-17 | 스티칭 허트 네덜란드 칸커 인스티튜트 | 종양성 질환의 치료를 위한 조성물 |
| EP2229434B1 (en) | 2007-11-27 | 2011-09-07 | Lifescan, Inc. | Differentiation of human embryonic stem cells |
| SG154367A1 (en) | 2008-01-31 | 2009-08-28 | Es Cell Int Pte Ltd | Method of differentiating stem cells |
| EP2250252A2 (en) | 2008-02-11 | 2010-11-17 | Cambridge Enterprise Limited | Improved reprogramming of mammalian cells, and the cells obtained |
| CN102046779A (zh) | 2008-02-21 | 2011-05-04 | 森托科尔奥索生物科技公司 | 用于细胞粘附、培养和分离的方法、表面改性培养板和组合物 |
| SG188918A1 (ru) * | 2008-03-17 | 2013-04-30 | Agency Science Tech & Res | |
| WO2009131568A1 (en) | 2008-04-21 | 2009-10-29 | Cythera, Inc. | Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells |
| US7939322B2 (en) * | 2008-04-24 | 2011-05-10 | Centocor Ortho Biotech Inc. | Cells expressing pluripotency markers and expressing markers characteristic of the definitive endoderm |
| WO2009137844A2 (en) * | 2008-05-09 | 2009-11-12 | Vistagen Therapeutics, Inc. | Pancreatic endocrine progenitor cells derived from pluripotent stem cells |
| US20090298178A1 (en) | 2008-06-03 | 2009-12-03 | D Amour Kevin Allen | Growth factors for production of definitive endoderm |
| PL2310492T3 (pl) * | 2008-06-30 | 2015-12-31 | Janssen Biotech Inc | Różnocowanie pluripotencjalnych komórek macierzystych |
| DE102008032236A1 (de) | 2008-06-30 | 2010-04-01 | Eberhard-Karls-Universität Tübingen | Isolierung und/oder Identifizierung von Stammzellen mit adipozytärem, chondrozytärem und pankreatischem Differenzierungspotential |
| US20100028307A1 (en) | 2008-07-31 | 2010-02-04 | O'neil John J | Pluripotent stem cell differentiation |
| AU2009308967C1 (en) | 2008-10-31 | 2017-04-20 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells to the pancreatic endocrine lineage |
| US8008075B2 (en) * | 2008-11-04 | 2011-08-30 | Viacyte, Inc. | Stem cell aggregate suspension compositions and methods of differentiation thereof |
| SG177416A1 (en) | 2009-07-20 | 2012-02-28 | Janssen Biotech Inc | Differentiation of human embryonic stem cells |
| CA2791846A1 (en) | 2010-03-02 | 2011-09-09 | National University Of Singapore | Culture additives to boost stem cell proliferation and differentiation response |
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| SG181822A1 (en) | 2012-08-30 |
| KR101764404B1 (ko) | 2017-08-03 |
| US9133439B2 (en) | 2015-09-15 |
| AU2010333840A1 (en) | 2012-07-12 |
| KR101923537B1 (ko) | 2018-11-29 |
| JP5882226B2 (ja) | 2016-03-09 |
| US20110151561A1 (en) | 2011-06-23 |
| RU2586506C2 (ru) | 2016-06-10 |
| WO2011079018A3 (en) | 2011-11-17 |
| PL2516626T3 (pl) | 2017-10-31 |
| EP2516626A4 (en) | 2013-06-26 |
| ES2633648T3 (es) | 2017-09-22 |
| EP2516626A2 (en) | 2012-10-31 |
| CN102712902A (zh) | 2012-10-03 |
| ZA201205488B (en) | 2016-01-27 |
| SG181685A1 (en) | 2012-07-30 |
| CA2784425A1 (en) | 2011-06-30 |
| EP2516626B1 (en) | 2017-05-10 |
| MX2012007414A (es) | 2012-07-17 |
| BR112012015727A2 (pt) | 2015-11-24 |
| KR101841271B1 (ko) | 2018-03-22 |
| KR20170090530A (ko) | 2017-08-07 |
| KR20120104386A (ko) | 2012-09-20 |
| MX339669B (es) | 2016-06-02 |
| RU2664864C1 (ru) | 2018-08-23 |
| WO2011079018A2 (en) | 2011-06-30 |
| AU2010333840B2 (en) | 2016-01-07 |
| CN102712902B (zh) | 2019-01-08 |
| KR20180030737A (ko) | 2018-03-23 |
| JP2013515481A (ja) | 2013-05-09 |
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