RS54482B1 - Biološka testiranja sa prostornim kodiranjem - Google Patents

Biološka testiranja sa prostornim kodiranjem

Info

Publication number
RS54482B1
RS54482B1 RS20150814A RSP20150814A RS54482B1 RS 54482 B1 RS54482 B1 RS 54482B1 RS 20150814 A RS20150814 A RS 20150814A RS P20150814 A RSP20150814 A RS P20150814A RS 54482 B1 RS54482 B1 RS 54482B1
Authority
RS
Serbia
Prior art keywords
probes
sample
targets
coding
nucleic acid
Prior art date
Application number
RS20150814A
Other languages
English (en)
Serbian (sr)
Inventor
Mark S. Chee
Original Assignee
Prognosys Biosciences, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44710331&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=RS54482(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Prognosys Biosciences, Inc. filed Critical Prognosys Biosciences, Inc.
Publication of RS54482B1 publication Critical patent/RS54482B1/sr

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6841In situ hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00Methods of screening libraries
    • C40B30/04Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/04Integrated apparatus specially adapted for both screening libraries and identifying library members
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845Methods of identifying protein-protein interactions in protein mixtures
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00Labels used in chemical analysis of biological material
    • G01N2458/10Oligonucleotides as tagging agents for labelling antibodies

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
  • Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
RS20150814A 2010-04-05 2011-04-05 Biološka testiranja sa prostornim kodiranjem RS54482B1 (sr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US32112410P 2010-04-05 2010-04-05
PCT/US2011/031308 WO2011127099A1 (en) 2010-04-05 2011-04-05 Spatially encoded biological assays
US13/080,616 US9371598B2 (en) 2010-04-05 2011-04-05 Spatially encoded biological assays

Publications (1)

Publication Number Publication Date
RS54482B1 true RS54482B1 (sr) 2016-06-30

Family

ID=44710331

Family Applications (1)

Application Number Title Priority Date Filing Date
RS20150814A RS54482B1 (sr) 2010-04-05 2011-04-05 Biološka testiranja sa prostornim kodiranjem

Country Status (18)

Country Link
US (21) US9371598B2 (OSRAM)
EP (1) EP2556171B1 (OSRAM)
JP (1) JP5893607B2 (OSRAM)
KR (1) KR101866401B1 (OSRAM)
CN (2) CN105385756B (OSRAM)
AU (1) AU2011237729B2 (OSRAM)
CA (1) CA2794522C (OSRAM)
DK (1) DK2556171T3 (OSRAM)
ES (1) ES2555106T3 (OSRAM)
HK (1) HK1222210A1 (OSRAM)
HR (1) HRP20151294T1 (OSRAM)
HU (1) HUE026666T2 (OSRAM)
PL (1) PL2556171T3 (OSRAM)
PT (1) PT2556171E (OSRAM)
RS (1) RS54482B1 (OSRAM)
SI (1) SI2556171T1 (OSRAM)
SM (1) SMT201500301B (OSRAM)
WO (1) WO2011127099A1 (OSRAM)

Families Citing this family (317)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010127186A1 (en) 2009-04-30 2010-11-04 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
RS54482B1 (sr) 2010-04-05 2016-06-30 Prognosys Biosciences, Inc. Biološka testiranja sa prostornim kodiranjem
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US20120258871A1 (en) 2011-04-08 2012-10-11 Prognosys Biosciences, Inc. Peptide constructs and assay systems
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US10501791B2 (en) 2011-10-14 2019-12-10 President And Fellows Of Harvard College Sequencing by structure assembly
ES2953308T3 (es) 2011-12-22 2023-11-10 Harvard College Composiciones y métodos para la detección de analitos
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
WO2014163886A1 (en) 2013-03-12 2014-10-09 President And Fellows Of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
GB2513024B (en) 2012-02-27 2016-08-31 Cellular Res Inc A clonal amplification method
US9914967B2 (en) 2012-06-05 2018-03-13 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using DNA origami probes
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
CN111748607B (zh) 2012-08-14 2024-04-30 10X基因组学有限公司 微胶囊组合物及方法
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
USRE50065E1 (en) 2012-10-17 2024-07-30 10X Genomics Sweden Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3736573A1 (en) 2013-03-15 2020-11-11 Prognosys Biosciences, Inc. Methods for detecting peptide/mhc/tcr binding
JPWO2014188941A1 (ja) * 2013-05-22 2017-02-23 オリンパス株式会社 核酸分析キットおよび核酸分析方法
MY177814A (en) 2013-06-04 2020-09-23 Harvard College Rna-guided transcriptional regulation
CN105849275B (zh) 2013-06-25 2020-03-17 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统
ES2857908T3 (es) 2013-08-28 2021-09-29 Becton Dickinson Co Análisis masivamente paralelo de células individuales
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US10288608B2 (en) 2013-11-08 2019-05-14 Prognosys Biosciences, Inc. Polynucleotide conjugates and methods for analyte detection
US9834814B2 (en) * 2013-11-22 2017-12-05 Agilent Technologies, Inc. Spatial molecular barcoding of in situ nucleic acids
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
CN106536756A (zh) 2014-06-26 2017-03-22 10X基因组学有限公司 核酸序列的分析
CN113249435B (zh) 2014-06-26 2024-09-03 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
US10179932B2 (en) 2014-07-11 2019-01-15 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
WO2016018960A1 (en) 2014-07-30 2016-02-04 President And Fellows Of Harvard College Systems and methods for determining nucleic acids
EP2983297A1 (en) * 2014-08-08 2016-02-10 Thomson Licensing Code generation method, code generating apparatus and computer readable storage medium
WO2016090148A1 (en) 2014-12-03 2016-06-09 IsoPlexis Corporation Analysis and screening of cell secretion profiles
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
ES2836802T3 (es) 2015-02-27 2021-06-28 Becton Dickinson Co Códigos de barras moleculares espacialmente direccionables
EP4180535A1 (en) 2015-03-30 2023-05-17 Becton, Dickinson and Company Methods and compositions for combinatorial barcoding
HK1250245A1 (zh) 2015-04-10 2018-12-07 Spatial Transcriptomics Ab 生物样本的空间区别、多重核酸分析
JP6875998B2 (ja) * 2015-04-14 2021-05-26 コーニンクレッカ フィリップス エヌ ヴェKoninklijke Philips N.V. 生物学的組織試料の分子プロファイルの空間マッピング
EP4582556A3 (en) 2015-04-23 2025-10-08 Becton, Dickinson and Company Methods and compositions for whole transcriptome amplification
WO2017015097A1 (en) 2015-07-17 2017-01-26 Nanostring Technologies, Inc. Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue
EP4368728A3 (en) 2015-07-17 2024-08-07 Bruker Spatial Biology, Inc. Simultaneous quantification of gene expression in a user-defined region of a cross-sectioned tissue
ES2888626T3 (es) * 2015-07-27 2022-01-05 Illumina Inc Cartografiado espacial de información de secuencia de ácidos nucleicos
ES2745694T3 (es) 2015-09-11 2020-03-03 Cellular Res Inc Métodos y composiciones para normalización de biblioteca de ácido nucleico
EP3368668B1 (en) 2015-10-28 2023-11-29 Silicon Valley Scientific, Inc. Method and apparatus for encoding cellular spatial position information
GB2559526B (en) 2015-11-03 2021-02-17 Harvard College Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
SG11201804086VA (en) 2015-12-04 2018-06-28 10X Genomics Inc Methods and compositions for nucleic acid analysis
EP4613756A3 (en) 2016-04-25 2025-11-12 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
DK3452591T5 (da) 2016-05-02 2024-09-09 Encodia Inc Makromolekyleanalyse under anvendelse af nukleinsyrekodning
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
EP4050112A1 (en) 2016-06-21 2022-08-31 10X Genomics, Inc. Nucleic acid sequencing
CN118853848A (zh) 2016-08-31 2024-10-29 哈佛学院董事及会员团体 将生物分子的检测组合到使用荧光原位测序的单个试验的方法
GB2570412A (en) 2016-08-31 2019-07-24 Harvard College Methods of generating libraries of nucleic acid sequences for detection via fluorescent in situ sequencing
WO2018049418A1 (en) 2016-09-12 2018-03-15 IsoPlexis Corporation System and methods for multiplexed analysis of cellular and other immunotherapeutics
KR102522023B1 (ko) 2016-09-26 2023-04-17 셀룰러 리서치, 인크. 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정
EP4036578A1 (en) 2016-11-11 2022-08-03 Isoplexis Corporation Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells
US11525783B2 (en) 2016-11-22 2022-12-13 IsoPlexis Corporation Systems, devices and methods for cell capture and methods of manufacture thereof
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
EP3545089B1 (en) 2017-01-30 2022-03-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
EP3577232A1 (en) 2017-02-01 2019-12-11 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
CN109789167A (zh) 2017-04-14 2019-05-21 哈佛学院董事及会员团体 用于产生细胞衍生的微丝网络的方法
US11788123B2 (en) 2017-05-26 2023-10-17 President And Fellows Of Harvard College Systems and methods for high-throughput image-based screening
KR102790050B1 (ko) 2017-06-05 2025-04-04 백톤 디킨슨 앤드 컴퍼니 단일 세포를 위한 샘플 인덱싱
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
EP4589017A3 (en) 2017-10-06 2025-10-08 10x Genomics, Inc. Rna templated ligation
CN114525273B (zh) 2017-10-27 2025-01-28 10X基因组学有限公司 用于样品制备和分析的方法和系统
US11513126B2 (en) 2017-10-31 2022-11-29 Encodia, Inc. Kits for analysis using nucleic acid encoding and/or label
CN111051523B (zh) 2017-11-15 2024-03-19 10X基因组学有限公司 功能化凝胶珠
EP3724658A1 (en) 2017-12-12 2020-10-21 10X Genomics, Inc. Systems and methods for single cell processing
CN118547046A (zh) 2017-12-22 2024-08-27 10X基因组学有限公司 用于处理来自一个或多个细胞的核酸分子的系统和方法
JP7372927B6 (ja) 2018-02-12 2023-11-27 ナノストリング テクノロジーズ,インコーポレイティド 遺伝子およびタンパク質の発現を検出する生体分子プローブおよびその検出方法
SG11202007686VA (en) 2018-02-12 2020-09-29 10X Genomics Inc Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
WO2019178164A1 (en) 2018-03-12 2019-09-19 Silicon Valley Scientific, Inc. Method and apparatus for processing tissue and other samples encoding cellular spatial position information
US12385033B2 (en) 2018-05-02 2025-08-12 The General Hospital Corporation High-resolution spatial macromolecule abundance assessment
WO2019213294A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
EP4545647A3 (en) 2018-05-03 2025-07-09 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
SG11202101934SA (en) 2018-07-30 2021-03-30 Readcoor Llc Methods and systems for sample processing or analysis
CN112703252B (zh) 2018-08-03 2024-09-10 10X基因组学有限公司 用于最小化条形码交换的方法和系统
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
EP4603594A3 (en) 2018-08-28 2025-11-12 10X Genomics, Inc. Increasing spatial array resolution
US20210317524A1 (en) 2018-08-28 2021-10-14 10X Genomics, Inc. Resolving spatial arrays
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
EP3844299A1 (en) * 2018-08-28 2021-07-07 Becton Dickinson and Company Sample multiplexing using carbohydrate-binding and membrane-permeable reagents
EP4471156A3 (en) 2018-10-01 2025-02-26 Becton, Dickinson and Company Determining 5' transcript sequences
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
JP7618548B2 (ja) 2018-11-08 2025-01-21 ベクトン・ディキンソン・アンド・カンパニー ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析
US11208648B2 (en) 2018-11-16 2021-12-28 International Business Machines Corporation Determining position and transcriptomes of biological cells
US12460253B2 (en) 2018-12-04 2025-11-04 Roche Sequencing Solutions, Inc. Spatially oriented quantum barcoding of cellular targets
WO2020123318A1 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Resolving spatial arrays using deconvolution
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
WO2020123384A1 (en) 2018-12-13 2020-06-18 Cellular Research, Inc. Selective extension in single cell whole transcriptome analysis
EP3894583A4 (en) 2018-12-13 2022-11-09 President and Fellows of Harvard College AMPLIFICATION METHODS AND SYSTEMS FOR MERFISH AND OTHER APPLICATIONS
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
ES2989967T3 (es) 2019-01-23 2024-11-28 Becton Dickinson Co Oligonucleótidos asociados con anticuerpos
US12275993B2 (en) 2019-02-12 2025-04-15 10X Genomics, Inc. Analysis of nucleic acid sequences
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US12305239B2 (en) 2019-02-12 2025-05-20 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
CN118979095A (zh) 2019-02-12 2024-11-19 10X基因组学有限公司 用于加工核酸分子的方法
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
WO2020167862A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transfer of reagents between droplets
US12071617B2 (en) 2019-02-14 2024-08-27 Becton, Dickinson And Company Hybrid targeted and whole transcriptome amplification
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
CN114174531A (zh) 2019-02-28 2022-03-11 10X基因组学有限公司 用空间条码化寡核苷酸阵列对生物分析物进行概况分析
CN113767178A (zh) 2019-03-11 2021-12-07 10X基因组学有限公司 用于处理光学标签化珠粒的系统和方法
EP3938538A1 (en) 2019-03-15 2022-01-19 10X Genomics, Inc. Methods for using spatial arrays for single cell sequencing
WO2020198071A1 (en) 2019-03-22 2020-10-01 10X Genomics, Inc. Three-dimensional spatial analysis
US12498378B2 (en) 2019-04-23 2025-12-16 Encodia, Inc. Methods for spatial analysis of proteins and related kits
JP7582676B2 (ja) 2019-04-30 2024-11-13 エンコディア, インコーポレイテッド 分析物を調製するための方法および関連キット
WO2020227543A2 (en) * 2019-05-07 2020-11-12 Fluidigm Corporation Sequence based imaging
SG11202112703QA (en) * 2019-05-15 2021-12-30 Bgi Shenzhen Array and method for detecting spatial information of nucleic acids
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
SG11202111878RA (en) 2019-05-31 2021-11-29 10X Genomics Inc Method of detecting target nucleic acid molecules
CN120099137A (zh) 2019-07-22 2025-06-06 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
EP4038546B1 (en) 2019-10-01 2024-08-21 10X Genomics, Inc. Systems and methods for identifying morphological patterns in tissue samples
US12157124B2 (en) 2019-11-06 2024-12-03 10X Genomics, Inc. Imaging system hardware
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
EP4055185A1 (en) 2019-11-08 2022-09-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
CN114729350A (zh) 2019-11-08 2022-07-08 贝克顿迪金森公司 使用随机引发获得用于免疫组库测序的全长v(d)j信息
EP4589016A3 (en) 2019-11-13 2025-10-08 10x Genomics, Inc. Generating capture probes for spatial analysis
US12154266B2 (en) 2019-11-18 2024-11-26 10X Genomics, Inc. Systems and methods for binary tissue classification
WO2021102039A1 (en) 2019-11-21 2021-05-27 10X Genomics, Inc, Spatial analysis of analytes
EP4435718A3 (en) 2019-11-22 2024-12-18 10X Genomics, Inc. Systems and methods for spatial analysis of analytes using fiducial alignment
WO2021119320A2 (en) 2019-12-11 2021-06-17 10X Genomics, Inc. Reverse transcriptase variants
GB201919032D0 (en) 2019-12-20 2020-02-05 Cartana Ab Method of detecting an analyte
EP4081656A1 (en) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
ES2982420T3 (es) 2019-12-23 2024-10-16 10X Genomics Inc Métodos para el análisis espacial mediante el uso de la ligazón con plantilla de ARN
EP4628596A3 (en) 2020-01-10 2025-12-31 10X Genomics, Inc. METHODS FOR DETERMINING THE LOCATION OF A TARGET NUCLEIC ACID IN A BIOLOGICAL SAMPLE
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110548B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Bi-directional in situ analysis
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12112833B2 (en) 2020-02-04 2024-10-08 10X Genomics, Inc. Systems and methods for index hopping filtering
US11732300B2 (en) * 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
CN115428088A (zh) 2020-02-13 2022-12-02 10X基因组学有限公司 用于基因表达和dna染色质可及性的联合交互式可视化的系统和方法
US12399123B1 (en) 2020-02-14 2025-08-26 10X Genomics, Inc. Spatial targeting of analytes
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
EP4107284A1 (en) 2020-02-17 2022-12-28 10X Genomics, Inc. In situ analysis of chromatin interaction
WO2021168278A1 (en) 2020-02-20 2021-08-26 10X Genomics, Inc. METHODS TO COMBINE FIRST AND SECOND STRAND cDNA SYNTHESIS FOR SPATIAL ANALYSIS
CN116157533A (zh) 2020-02-21 2023-05-23 10X基因组学有限公司 使用杂交方法捕获遗传靶标
EP4107285B1 (en) 2020-02-21 2024-10-09 10X Genomics, Inc. Methods and compositions for integrated in situ spatial assay
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
WO2021173719A1 (en) 2020-02-25 2021-09-02 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
ES2965354T3 (es) 2020-04-22 2024-04-12 10X Genomics Inc Métodos para análisis espacial que usan eliminación de ARN elegido como diana
WO2021225900A1 (en) 2020-05-04 2021-11-11 10X Genomics, Inc. Spatial transcriptomic transfer modes
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
US12416603B2 (en) 2020-05-19 2025-09-16 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021237056A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Rna integrity analysis in a biological sample
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
EP4600376A3 (en) 2020-06-02 2025-10-22 10X Genomics, Inc. Spatial transcriptomics for antigen-receptors
EP4158055B1 (en) 2020-06-02 2024-03-27 Becton, Dickinson and Company Oligonucleotides and beads for 5 prime gene expression assay
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
AU2021283174A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Nucleic acid library methods
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4421186B1 (en) 2020-06-08 2025-08-13 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
US12435363B1 (en) 2020-06-10 2025-10-07 10X Genomics, Inc. Materials and methods for spatial transcriptomics
ES2999535T3 (en) 2020-06-10 2025-02-26 10X Genomics Inc Methods for determining a location of an analyte in a biological sample
WO2021252576A1 (en) 2020-06-10 2021-12-16 10X Genomics, Inc. Methods for spatial analysis using blocker oligonucleotides
AU2021288090A1 (en) 2020-06-10 2023-01-19 10X Genomics, Inc. Fluid delivery methods
US12209273B2 (en) 2020-06-12 2025-01-28 10X Genomics, Inc. Nucleic acid assays using click chemistry bioconjugation
WO2021263111A1 (en) 2020-06-25 2021-12-30 10X Genomics, Inc. Spatial analysis of dna methylation
US12168801B1 (en) 2020-07-02 2024-12-17 10X Genomics, Inc. Hybrid/capture probe designs for full-length cDNA
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
CN116323968A (zh) 2020-07-31 2023-06-23 10X基因组学有限公司 用于空间分析的去交联化合物和使用方法
WO2022032194A1 (en) 2020-08-06 2022-02-10 Singular Genomics Systems, Inc. Methods for in situ transcriptomics and proteomics
US11434525B2 (en) 2020-08-06 2022-09-06 Singular Genomics Systems, Inc. Spatial sequencing
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
US12297499B2 (en) 2020-08-17 2025-05-13 10X Genomics, Inc. Multicomponent nucleic acid probes for sample analysis
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
CN116547388A (zh) 2020-09-15 2023-08-04 10X基因组学有限公司 从基材中释放延伸的捕获探针的方法及其应用
WO2022060953A1 (en) 2020-09-16 2022-03-24 10X Genomics, Inc. Methods of determining the location of an analyte in a biological sample using a plurality of wells
ES2993269T3 (en) 2020-09-18 2024-12-26 10X Genomics Inc Sample handling apparatus and image registration methods
WO2022061152A2 (en) 2020-09-18 2022-03-24 10X Genomics, Inc. Sample handling apparatus and fluid delivery methods
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
WO2022081643A2 (en) 2020-10-13 2022-04-21 10X Genomics, Inc. Compositions and methods for generating recombinant antigen binding molecules from single cells
AU2021366701A1 (en) 2020-10-22 2023-05-04 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
US12480158B1 (en) 2020-11-05 2025-11-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
EP4222283A1 (en) 2020-11-06 2023-08-09 10X Genomics, Inc. Compositions and methods for binding an analyte to a capture probe
WO2022109181A1 (en) 2020-11-18 2022-05-27 10X Genomics, Inc. Methods and compositions for analyzing immune infiltration in cancer stroma to predict clinical outcome
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
EP4012046A1 (en) 2020-12-11 2022-06-15 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US20240076723A1 (en) 2020-12-30 2024-03-07 10X Genomics, Inc. Cleavage of capture probes for spatial analysis
WO2022147005A1 (en) 2020-12-30 2022-07-07 10X Genomics, Inc. Methods for analyte capture determination
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
US12398262B1 (en) 2021-01-22 2025-08-26 10X Genomics, Inc. Triblock copolymer-based cell stabilization and fixation system and methods of use thereof
EP4284942B1 (en) 2021-01-29 2024-08-14 10X Genomics, Inc. Method for transposase mediated spatial tagging and analyzing genomic dna in a biological sample
EP4421491A3 (en) 2021-02-19 2024-11-27 10X Genomics, Inc. Method of using a modular assay support device
WO2022182682A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
US12275984B2 (en) 2021-03-02 2025-04-15 10X Genomics, Inc. Sequential hybridization and quenching
EP4524261A3 (en) 2021-03-18 2025-05-14 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
AU2022256031A1 (en) 2021-04-05 2023-10-12 10X Genomics, Inc. Recombinant ligase composition and uses thereof
EP4305196B1 (en) 2021-04-14 2025-04-02 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
US20240218432A1 (en) 2021-04-20 2024-07-04 10X Genomics, Inc. Methods for assessing sample quality prior to spatial analysis using templated ligation
WO2022232571A1 (en) 2021-04-30 2022-11-03 10X Genomics, Inc. Fusion rt variants for improved performance
EP4334706A4 (en) 2021-05-05 2025-08-06 Singular Genomics Systems Inc MULTIOMICS ANALYSIS DEVICE AND METHODS OF USE THEREOF
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
ES3030033T3 (en) 2021-06-03 2025-06-26 10X Genomics Inc Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
WO2022265965A1 (en) 2021-06-14 2022-12-22 10X Genomics, Inc. Reverse transcriptase variants for improved performance
US20240368711A1 (en) 2021-06-22 2024-11-07 10X Genomics, Inc. Spatial detection of sars-cov-2 using templated ligation
EP4108783B1 (en) * 2021-06-24 2024-02-21 Miltenyi Biotec B.V. & Co. KG Spatial sequencing with mictag
CN117616134A (zh) * 2021-07-01 2024-02-27 美天施生物科技有限两合公司 用于空间条形码编码和测序的unit-dna组合物
US20230026886A1 (en) 2021-07-13 2023-01-26 10X Genomics, Inc. Methods for preparing polymerized matrix with controllable thickness
WO2023287765A1 (en) 2021-07-13 2023-01-19 10X Genomics, Inc. Methods for spatial analysis using targeted probe silencing
US12360105B2 (en) 2021-07-30 2025-07-15 10X Genomics, Inc. Methods and compositions for synchronizing reactions in situ
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
US12460251B2 (en) 2021-08-03 2025-11-04 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid molecules
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
WO2023018799A1 (en) 2021-08-12 2023-02-16 10X Genomics, Inc. Methods, compositions and systems for identifying antigen-binding molecules
CN117858958A (zh) 2021-08-16 2024-04-09 10X基因组学有限公司 包含分割条形码区的探针和使用方法
ES3011462T3 (en) 2021-09-01 2025-04-07 10X Genomics Inc Methods for blocking a capture probe on a spatial array
WO2023044071A1 (en) 2021-09-17 2023-03-23 10X Genomics, Inc. Systems and methods for image registration or alignment
WO2023076345A1 (en) 2021-10-26 2023-05-04 10X Genomics, Inc. Methods for spatial analysis using targeted rna capture
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
US20250147036A1 (en) 2021-11-10 2025-05-08 10X Genomics, Inc. Methods for identification of antigen-binding molecules
EP4441741A1 (en) 2021-11-30 2024-10-09 10X Genomics, Inc. Systems and methods for identifying regions of aneuploidy in a tissue
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
WO2023114473A2 (en) 2021-12-16 2023-06-22 10X Genomics, Inc. Recombinant reverse transcriptase variants for improved performance
EP4441711A1 (en) 2021-12-20 2024-10-09 10X Genomics, Inc. Self-test for pathology/histology slide imaging device
EP4466376A1 (en) 2022-01-21 2024-11-27 10X Genomics, Inc. Multiple readout signals for analyzing a sample
WO2023150171A1 (en) 2022-02-01 2023-08-10 10X Genomics, Inc. Methods, compositions, and systems for capturing analytes from glioblastoma samples
WO2023150098A1 (en) 2022-02-01 2023-08-10 10X Genomics, Inc. Methods, kits, compositions, and systems for spatial analysis
WO2023150163A1 (en) 2022-02-01 2023-08-10 10X Genomics, Inc. Methods, compositions, and systems for capturing analytes from lymphatic tissue
WO2023159028A1 (en) 2022-02-15 2023-08-24 10X Genomics, Inc. Systems and methods for spatial analysis of analytes using fiducial alignment
CN118974836A (zh) 2022-03-08 2024-11-15 10X基因组学有限公司 将光学拥挤最小化的原位代码设计方法
WO2023172670A2 (en) 2022-03-11 2023-09-14 10X Genomics, Inc. Sample handling apparatus and fluid delivery methods
AU2023237896A1 (en) 2022-03-24 2024-10-17 Digital Biology Inc. Tissue spatial omics
EP4505177A1 (en) 2022-04-01 2025-02-12 10x Genomics, Inc. Compositions and methods for targeted masking of autofluorescence
US20250251403A1 (en) 2022-04-12 2025-08-07 10X Genomics, Inc. Compositions and methods for generating and characterizing recombinant antigen binding molecules
US20250250632A1 (en) 2022-05-06 2025-08-07 10X Genomics, Inc. Molecular barcode readers for analyte detection
WO2023215603A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Methods and compositions for in situ analysis of v(d)j sequences
US20240002902A1 (en) 2022-05-06 2024-01-04 10X Genomics, Inc. Analysis of antigen and antigen receptor interactions
US20240084378A1 (en) 2022-05-11 2024-03-14 10X Genomics, Inc. Compositions and methods for in situ sequencing
WO2023225519A1 (en) 2022-05-17 2023-11-23 10X Genomics, Inc. Modified transposons, compositions and uses thereof
WO2023229988A1 (en) 2022-05-23 2023-11-30 10X Genomics, Inc. Tissue sample mold
WO2023229982A2 (en) 2022-05-24 2023-11-30 10X Genomics, Inc. Porous structure confinement for convection suppression
EP4540607A1 (en) 2022-06-17 2025-04-23 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
WO2023250077A1 (en) 2022-06-22 2023-12-28 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
WO2024015862A1 (en) 2022-07-13 2024-01-18 10X Genomics, Inc. Methods for characterization of antigen-binding molecules from biological samples
WO2024015578A1 (en) 2022-07-15 2024-01-18 10X Genomics, Inc. Methods for determining a location of a target nucleic acid in a biological sample
AU2023308992A1 (en) 2022-07-19 2025-01-30 Digital Biology Inc. Barcode diffusion-based spatial omics
US20240052404A1 (en) 2022-08-05 2024-02-15 10X Genomics, Inc. Systems and methods for immunofluorescence quantification
WO2024036191A1 (en) 2022-08-10 2024-02-15 10X Genomics, Inc. Systems and methods for colocalization
WO2024035844A1 (en) 2022-08-12 2024-02-15 10X Genomics, Inc. Methods for reducing capture of analytes
CN119677873A (zh) 2022-08-16 2025-03-21 10X基因组学有限公司 Ap50聚合酶及其用途
EP4577670A1 (en) 2022-08-24 2025-07-02 10X Genomics, Inc. Compositions and methods for antigenic epitope mapping in biological samples
WO2024081212A1 (en) 2022-10-10 2024-04-18 10X Genomics, Inc. In vitro transcription of spatially captured nucleic acids
CN120129825A (zh) 2022-10-14 2025-06-10 10X基因组学有限公司 用于评估生物样品质量的方法、组合物和系统
WO2024086167A2 (en) 2022-10-17 2024-04-25 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
EP4482979B1 (en) 2022-11-09 2025-10-01 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample
EP4638788A1 (en) 2022-12-21 2025-10-29 10X Genomics, Inc. Analysis of analytes and spatial gene expression
WO2024145224A1 (en) 2022-12-29 2024-07-04 10X Genomics, Inc. Compositions, methods, and systems for high resolution spatial analysis
WO2024145441A1 (en) 2022-12-29 2024-07-04 10X Genomics, Inc. Methods, compositions, and kits for determining a location of a target nucleic acid in a fixed biological sample
WO2024145445A1 (en) 2022-12-30 2024-07-04 10X Genomics, Inc. Methods of capturing target analytes
EP4486913B1 (en) 2022-12-30 2025-07-16 10x Genomics, Inc. Methods, compositions, and kits for multiple barcoding and/or high-density spatial barcoding
WO2024206603A1 (en) 2023-03-28 2024-10-03 10X Genomics, Inc. Methods, compositions, and kits for reducing analyte mislocalization
US20250257393A1 (en) 2023-04-21 2025-08-14 10X Genomics, Inc. Methods and compositions for detecting nucleic acids in a fixed biological sample
EP4500178A1 (en) 2023-05-15 2025-02-05 10x Genomics, Inc. Spatial antibody data normalization
WO2024238900A1 (en) 2023-05-17 2024-11-21 10X Genomics, Inc. Methods of reducing mislocalization of an analyte
WO2024238992A1 (en) 2023-05-18 2024-11-21 10X Genomics, Inc. Engineered non-strand displacing family b polymerases for reverse transcription and gap-fill applications
WO2024249961A1 (en) 2023-06-01 2024-12-05 Singular Genomics Systems, Inc. Methods and probes for detecting polynucleotide sequences in cells and tissues
WO2024254316A1 (en) 2023-06-07 2024-12-12 10X Genomics, Inc. Compositions and methods for identifying blood clots in biological samples
WO2024258911A1 (en) 2023-06-13 2024-12-19 10X Genomics, Inc. Sequence specific dna binding proteins
WO2025029605A1 (en) 2023-07-28 2025-02-06 10X Genomics, Inc. Methods and compositions for detecting genetic variants using capture probes and primer extension
US12319956B2 (en) 2023-07-31 2025-06-03 10X Genomics, Inc. Methods and systems for targeted RNA cleavage and target RNA-primed rolling circle amplification
WO2025029627A1 (en) 2023-07-31 2025-02-06 10X Genomics, Inc. Methods, compositions, and kits for spatial detection of genetic variants
EP4581160A1 (en) 2023-08-24 2025-07-09 10X Genomics, Inc. Methods, kits, and compositions for spatial detection of genetic variants
WO2025072119A1 (en) 2023-09-26 2025-04-03 10X Genomics, Inc. Methods, compositions, and kits for detecting spatial chromosomal interactions
WO2025090912A1 (en) 2023-10-26 2025-05-01 10X Genomics, Inc. Spatial arrays containing reusable master probes
WO2025096581A1 (en) 2023-10-31 2025-05-08 10X Genomics, Inc. Methods of spatial analysis and single nuclei sequencing
WO2025101377A1 (en) 2023-11-09 2025-05-15 10X Genomics, Inc. Matrix-assisted spatial analysis of biological samples
WO2025101864A1 (en) 2023-11-09 2025-05-15 10X Genomics, Inc. Methods, compositions, and kits for reducing mislocalization of analytes in spatial analysis assays
WO2025255457A1 (en) 2024-06-07 2025-12-11 10X Genomics, Inc. Compositions and methods for improved on-target spatial profiling

Family Cites Families (557)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4883867A (en) 1985-11-01 1989-11-28 Becton, Dickinson And Company Detection of reticulocytes, RNA or DNA
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5589173A (en) 1986-11-04 1996-12-31 Genentech, Inc. Method and therapeutic compositions for the treatment of myocardial infarction
GB8810400D0 (en) 1988-05-03 1988-06-08 Southern E Analysing polynucleotide sequences
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
AU2684488A (en) 1988-06-27 1990-01-04 Carter-Wallace, Inc. Test device and method for colored particle immunoassay
US5512439A (en) 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
US5002882A (en) 1989-04-27 1991-03-26 New England Biolabs, Inc. Method for producing the XmaI restriction endonuclease and methylase
RU2145635C1 (ru) 1989-05-18 2000-02-20 Чирон Корпорейшн Олигомер (варианты), способ обнаружения последовательности hcv (варианты), набор для обнаружения, способ получения крови
CA2044616A1 (en) 1989-10-26 1991-04-27 Roger Y. Tsien Dna sequencing
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5559032A (en) 1990-06-29 1996-09-24 Pomeroy; Patrick C. Method and apparatus for post-transfer assaying of material on solid support
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5183053A (en) 1991-04-12 1993-02-02 Acuderm, Inc. Elliptical biopsy punch
US5474796A (en) 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
WO1993006482A1 (en) 1991-09-16 1993-04-01 Molecular Probes, Inc. Dimers of unsymmetrical cyanine dyes
US5321130A (en) 1992-02-10 1994-06-14 Molecular Probes, Inc. Unsymmetrical cyanine dyes with a cationic side chain
US5308751A (en) 1992-03-23 1994-05-03 General Atomics Method for sequencing double-stranded DNA
US5503980A (en) 1992-11-06 1996-04-02 Trustees Of Boston University Positional sequencing by hybridization
US5472881A (en) 1992-11-12 1995-12-05 University Of Utah Research Foundation Thiol labeling of DNA for attachment to gold surfaces
US5410030A (en) 1993-04-05 1995-04-25 Molecular Probes, Inc. Dimers of unsymmetrical cyanine dyes containing pyridinium moieties
US5436134A (en) 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5658751A (en) 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5837832A (en) 1993-06-25 1998-11-17 Affymetrix, Inc. Arrays of nucleic acid probes on biological chips
EP0711303B2 (en) 1993-07-30 2009-06-10 Affymax, Inc. Biotinylation of proteins
US6401267B1 (en) 1993-09-27 2002-06-11 Radoje Drmanac Methods and compositions for efficient nucleic acid sequencing
US5610287A (en) 1993-12-06 1997-03-11 Molecular Tool, Inc. Method for immobilizing nucleic acid molecules
SE9400522D0 (sv) 1994-02-16 1994-02-16 Ulf Landegren Method and reagent for detecting specific nucleotide sequences
US5512462A (en) 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
JPH09510351A (ja) 1994-03-16 1997-10-21 ジェン−プローブ・インコーポレイテッド 等温鎖置換核酸増幅法
US6015880A (en) 1994-03-16 2000-01-18 California Institute Of Technology Method and substrate for performing multiple sequential reactions on a matrix
US5552278A (en) 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
EP0777749B1 (en) 1994-08-19 2002-10-30 PE Corporation (NY) Coupled amplification and ligation method
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
KR960022566A (ko) 1994-12-30 1996-07-18 김충환 신규한 아미노올리고당 유도체 및 그의 제조방법
US5736351A (en) 1995-01-09 1998-04-07 New Horizons Diagnostics Corporation Method for detection of contaminants
US5959098A (en) 1996-04-17 1999-09-28 Affymetrix, Inc. Substrate preparation process
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5648245A (en) 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
US5928928A (en) 1995-06-07 1999-07-27 Universiteit Van Amsterdam Human chitinase, its recombinant production, its use for decomposing chitin, its use in therapy or prophylaxis against infection diseases
SE504798C2 (sv) 1995-06-16 1997-04-28 Ulf Landegren Immunanalys och testkit med två reagens som kan tvärbindas om de adsorberats till analyten
JP4709333B2 (ja) 1995-10-13 2011-06-22 プレジデント・アンド・フエローズ・オブ・ハーバード・カレツジ ホスホパンテテイニルトランスフェラーゼおよびその使用
US5716825A (en) 1995-11-01 1998-02-10 Hewlett Packard Company Integrated nucleic acid analysis system for MALDI-TOF MS
US5763175A (en) 1995-11-17 1998-06-09 Lynx Therapeutics, Inc. Simultaneous sequencing of tagged polynucleotides
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US6300063B1 (en) 1995-11-29 2001-10-09 Affymetrix, Inc. Polymorphism detection
EP0880598A4 (en) * 1996-01-23 2005-02-23 Affymetrix Inc RAPID EVALUATION OF NUCLEIC ACID ABUNDANCE DIFFERENCE, WITH A HIGH-DENSITY OLIGONUCLEOTIDE SYSTEM
EP2368897B1 (en) 1996-02-09 2016-10-19 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6013440A (en) 1996-03-11 2000-01-11 Affymetrix, Inc. Nucleic acid affinity columns
EP0912761A4 (en) 1996-05-29 2004-06-09 Cornell Res Foundation Inc DETERMINATION OF DIFFERENCES IN THE NUCLEIC ACID SEQUENCE BY MEANS OF A COMBINATION OF LIGASE DETERMINATION AND POLYMERASE CHAIN REACTION
US20050003431A1 (en) 1996-08-16 2005-01-06 Wucherpfennig Kai W. Monovalent, multivalent, and multimeric MHC binding domain fusion proteins and conjugates, and uses therefor
US5925545A (en) 1996-09-09 1999-07-20 Wisconsin Alumni Research Foundation System for in vitro transposition
US5965443A (en) 1996-09-09 1999-10-12 Wisconsin Alumni Research Foundation System for in vitro transposition
DE19639673A1 (de) 1996-09-27 1998-04-09 Daimler Benz Ag In einem Kraftfahrzeug im Bereich der Frontscheibe angeordnetes Display
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
US6083761A (en) 1996-12-02 2000-07-04 Glaxo Wellcome Inc. Method and apparatus for transferring and combining reagents
US6060240A (en) 1996-12-13 2000-05-09 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
US5837466A (en) 1996-12-16 1998-11-17 Vysis, Inc. Devices and methods for detecting nucleic acid analytes in samples
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
WO1998030575A1 (en) 1997-01-08 1998-07-16 Proligo Llc Bioconjugation of macromolecules
US6309824B1 (en) 1997-01-16 2001-10-30 Hyseq, Inc. Methods for analyzing a target nucleic acid using immobilized heterogeneous mixtures of oligonucleotide probes
CA2278786C (en) 1997-01-21 2010-07-20 The General Hospital Corporation Selection of proteins using rna-protein fusions
US8207093B2 (en) 1997-01-21 2012-06-26 The General Hospital Corporation Selection of proteins using RNA-protein fusions
US6261804B1 (en) 1997-01-21 2001-07-17 The General Hospital Corporation Selection of proteins using RNA-protein fusions
US5837860A (en) 1997-03-05 1998-11-17 Molecular Tool, Inc. Covalent attachment of nucleic acid molecules onto solid-phases via disulfide bonds
US6023540A (en) 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
US7622294B2 (en) * 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US6327410B1 (en) 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
US20020055100A1 (en) 1997-04-01 2002-05-09 Kawashima Eric H. Method of nucleic acid sequencing
WO1998044151A1 (en) 1997-04-01 1998-10-08 Glaxo Group Limited Method of nucleic acid amplification
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
US5919626A (en) 1997-06-06 1999-07-06 Orchid Bio Computer, Inc. Attachment of unmodified nucleic acids to silanized solid phase surfaces
WO1999005295A1 (en) 1997-07-25 1999-02-04 Thomas Jefferson University Composition and method for targeted integration into cells
GB9718455D0 (en) 1997-09-02 1997-11-05 Mcgregor Duncan P Chimeric binding peptide library screening method
AU737174B2 (en) 1997-10-10 2001-08-09 President & Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6242246B1 (en) 1997-12-15 2001-06-05 Somalogic, Inc. Nucleic acid ligand diagnostic Biochip
EP1600512B1 (en) 1997-12-22 2008-05-28 Hitachi Chemical Co., Ltd. mRNA purification on microplates
US6844158B1 (en) 1997-12-22 2005-01-18 Hitachi Chemical Co., Ltd. Direct RT-PCR on oligonucleotide-immobilized PCR microplates
US7427678B2 (en) 1998-01-08 2008-09-23 Sigma-Aldrich Co. Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method
WO1999042813A1 (en) 1998-02-23 1999-08-26 Wisconsin Alumni Research Foundation Method and apparatus for synthesis of arrays of dna probes
US6699710B1 (en) 1998-02-25 2004-03-02 The United States Of America As Represented By The Department Of Health And Human Services Tumor tissue microarrays for rapid molecular profiling
AU3567099A (en) 1998-04-16 1999-11-01 Packard Bioscience Company Analysis of polynucleotide sequence
US6358475B1 (en) 1998-05-27 2002-03-19 Becton, Dickinson And Company Device for preparing thin liquid for microscopic analysis
AU4333799A (en) 1998-06-04 1999-12-20 Board Of Regents, The University Of Texas System Digital optical chemistry micromirror imager
AU754952B2 (en) 1998-06-24 2002-11-28 Illumina, Inc. Decoding of array sensors with microspheres
AU4355899A (en) 1998-06-26 2000-01-17 Visible Genetics Inc. Method for sequencing nucleic acids with reduced errors
US20030022207A1 (en) 1998-10-16 2003-01-30 Solexa, Ltd. Arrayed polynucleotides and their use in genome analysis
US20040106110A1 (en) 1998-07-30 2004-06-03 Solexa, Ltd. Preparation of polynucleotide arrays
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6159736A (en) 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6573043B1 (en) 1998-10-07 2003-06-03 Genentech, Inc. Tissue analysis and kits therefor
WO2000024940A1 (en) 1998-10-28 2000-05-04 Vysis, Inc. Cellular arrays and methods of detecting and using genetic disorder markers
US6391937B1 (en) 1998-11-25 2002-05-21 Motorola, Inc. Polyacrylamide hydrogels and hydrogel arrays made from polyacrylamide reactive prepolymers
HK1039355B (en) 1998-12-02 2007-09-21 Bristol-Myers Squibb Company Dna-protein fusions and uses thereof
US6818418B1 (en) 1998-12-10 2004-11-16 Compound Therapeutics, Inc. Protein scaffolds for antibody mimics and other binding proteins
EP1141409B2 (en) 1998-12-14 2009-05-27 Pacific Biosciences of California, Inc. A kit and methods for nucleic acid sequencing of single molecules by polymerase synthesis
ATE440148T1 (de) 1999-01-06 2009-09-15 Callida Genomics Inc Verbesserte sequenzierung mittels hybridisierung durch verwendung von sondengemischen
GB9901475D0 (en) 1999-01-22 1999-03-17 Pyrosequencing Ab A method of DNA sequencing
US6565727B1 (en) 1999-01-25 2003-05-20 Nanolytics, Inc. Actuators for microfluidics without moving parts
US20020150909A1 (en) 1999-02-09 2002-10-17 Stuelpnagel John R. Automated information processing in randomly ordered arrays
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
US6153389A (en) 1999-02-22 2000-11-28 Haarer; Brian K. DNA additives as a mechanism for unambiguously marking biological samples
US6673620B1 (en) 1999-04-20 2004-01-06 Cytologix Corporation Fluid exchange in a chamber on a microscope slide
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
DK2264189T3 (en) 1999-04-20 2014-12-08 Illumina Inc Detection of nucleic acid reactions of bead - arrays
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US20050181440A1 (en) 1999-04-20 2005-08-18 Illumina, Inc. Nucleic acid sequencing using microsphere arrays
US7276336B1 (en) 1999-07-22 2007-10-02 Agilent Technologies, Inc. Methods of fabricating an addressable array of biopolymer probes
US20010055764A1 (en) 1999-05-07 2001-12-27 Empedocles Stephen A. Microarray methods utilizing semiconductor nanocrystals
WO2000075373A2 (en) * 1999-05-20 2000-12-14 Illumina, Inc. Combinatorial decoding of random nucleic acid arrays
US6544732B1 (en) * 1999-05-20 2003-04-08 Illumina, Inc. Encoding and decoding of array sensors utilizing nanocrystals
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6465183B2 (en) * 1999-07-01 2002-10-15 Agilent Technologies, Inc. Multidentate arrays
JP4137444B2 (ja) 1999-07-26 2008-08-20 ザ ガバメント オブ ザ ユナイテッドステイツ オブ アメリカ アズ リプレゼンテッド バイ ザ セクレタリー デパートメント オブ ヘルス アンド ヒューマン サービシーズ ザ ナショナル インステ 細胞分析用の捕捉領域を有する層状装置
WO2001009363A1 (en) 1999-08-02 2001-02-08 Wisconsin Alumni Research Foundation Mutant tn5 transposase enzymes and method for their use
EP1206577B1 (en) 1999-08-13 2006-03-01 Yale University Binary encoded sequence tags
EP1218545B1 (en) 1999-08-18 2012-01-25 Illumina, Inc. Methods for preparing oligonucleotide solutions
CA2382436C (en) 1999-08-30 2011-05-17 Illumina, Inc. Methods for improving signal detection from an array
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6291180B1 (en) 1999-09-29 2001-09-18 American Registry Of Pathology Ultrasound-mediated high-speed biological reaction and tissue processing
WO2001023610A2 (en) 1999-09-29 2001-04-05 Solexa Ltd. Polynucleotide sequencing
WO2001027327A2 (en) 1999-10-08 2001-04-19 Protogene Laboratories, Inc. Method and apparatus for performing large numbers of reactions using array assembly
US7585632B2 (en) 1999-10-29 2009-09-08 Hologic, Inc. Compositions and methods for the detection of a nucleic acid using a cleavage reaction
US6569674B1 (en) 1999-12-15 2003-05-27 Amersham Biosciences Ab Method and apparatus for performing biological reactions on a substrate surface
EP1238286A1 (en) 1999-12-13 2002-09-11 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES High-throughput tissue microarray technology and applications
US6485926B2 (en) 1999-12-22 2002-11-26 Fuji Photo Film Co., Ltd. Method for measuring protease activity
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US8076063B2 (en) 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
DE60136166D1 (de) 2000-02-07 2008-11-27 Illumina Inc Nukleinsäure-nachweisverfahren mit universellem priming
AU3806701A (en) 2000-02-07 2001-08-14 Illumina Inc Nucleic acid detection methods using universal priming
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7361488B2 (en) 2000-02-07 2008-04-22 Illumina, Inc. Nucleic acid detection methods using universal priming
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
CA2388738A1 (en) 2000-02-15 2001-08-23 Lynx Therapeutics, Inc. Data analysis and display system for ligation-based dna sequencing
US7306904B2 (en) 2000-02-18 2007-12-11 Olink Ab Methods and kits for proximity probing
WO2001077668A2 (en) 2000-04-10 2001-10-18 The Scripps Research Institute Proteomic analysis using active-site directed probes
US6368801B1 (en) 2000-04-12 2002-04-09 Molecular Staging, Inc. Detection and amplification of RNA using target-mediated ligation of DNA by RNA ligase
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
AU2001274869A1 (en) 2000-05-20 2001-12-03 The Regents Of The University Of Michigan Method of producing a dna library using positional amplification
JP4744778B2 (ja) 2000-06-21 2011-08-10 バイオアレイ ソルーションズ リミテッド 特定のランダム粒子アレイを適用した複数の被検体分子の分析方法
AU2001282881B2 (en) 2000-07-07 2007-06-14 Visigen Biotechnologies, Inc. Real-time sequence determination
GB0018120D0 (en) 2000-07-24 2000-09-13 Fermentas Ab Nuclease
AU2001280796A1 (en) 2000-07-25 2002-02-05 The Regents Of The University Of California Electrowetting-driven micropumping
US7613571B2 (en) 2000-07-28 2009-11-03 Doyle Michael D Method and system for the multidimensional morphological reconstruction of genome expression activity
AU7861301A (en) 2000-08-15 2002-02-25 Discerna Ltd Functional protein arrays
DK1731912T3 (da) 2000-08-21 2014-01-06 Apitope Technology Bristol Ltd Fremgangmåde til peptidselektion
US6713257B2 (en) 2000-08-25 2004-03-30 Rosetta Inpharmatics Llc Gene discovery using microarrays
US6773566B2 (en) 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
US20020168639A1 (en) 2000-09-22 2002-11-14 Muraca Patrick J. Profile array substrates
US6897023B2 (en) 2000-09-27 2005-05-24 The Molecular Sciences Institute, Inc. Method for determining relative abundance of nucleic acid sequences
AT411066B (de) 2000-10-24 2003-09-25 Steiner Georg E Verfahren und anordnung zur untersuchung von zellen
WO2002061129A2 (en) * 2000-11-15 2002-08-08 Minerva Biotechnologies Corporation Oligonucleotide identifiers
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US20030215936A1 (en) 2000-12-13 2003-11-20 Olli Kallioniemi High-throughput tissue microarray technology and applications
US20030017451A1 (en) 2000-12-21 2003-01-23 Hui Wang Methods for detecting transcripts
JP4061043B2 (ja) 2000-12-28 2008-03-12 株式会社ポストゲノム研究所 invitro転写/翻訳系によるペプチド等の製造方法
US6800453B2 (en) 2001-01-23 2004-10-05 President And Fellows Of Harvard College Nucleic-acid programmable protein arrays
WO2002059354A2 (en) 2001-01-25 2002-08-01 Tm Bioscience Corporation Polynucleotides for use as tags and tag complements, manufacture and use thereof
US20030087232A1 (en) 2001-01-25 2003-05-08 Fred Christians Methods for screening polypeptides
KR20020063359A (ko) 2001-01-27 2002-08-03 일렉트론 바이오 (주) 핵산 및 올리거뉴클레오티드의 상보적 이중결합의 특정서열에 특이적으로 반응하는 절단기법을 이용한 핵산 혼성분석 방법 및 장치
US20070020625A1 (en) 2001-02-07 2007-01-25 Eric Duchaud Sequence of the photorhabdus luminescens strain tt01 genome and uses
US6573051B2 (en) 2001-03-09 2003-06-03 Molecular Staging, Inc. Open circle probes with intramolecular stem structures
EP1368497A4 (en) 2001-03-12 2007-08-15 California Inst Of Techn METHODS AND APPARATUS FOR ANALYZING ASYNCHRONOUS BASE EXTENSION POLYNUCLEOTIDE SEQUENCES
AU785425B2 (en) 2001-03-30 2007-05-17 Genetic Technologies Limited Methods of genomic analysis
US6933117B2 (en) 2001-04-30 2005-08-23 Ventana Medical Systems, Inc. Reagents and methods for automated hybridization
AU2002322457A1 (en) 2001-06-28 2003-03-03 Illumina, Inc. Multiplex decoding of array sensors with microspheres
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
US20040241688A1 (en) 2001-07-19 2004-12-02 Cuneyt Bukusoglu Human tissue specific drug screening procedure
GB0118031D0 (en) 2001-07-24 2001-09-19 Oxford Glycosciences Uk Ltd Self assembled protein nucleic acid complexes and self assembled protein arrays
US7297778B2 (en) 2001-07-25 2007-11-20 Affymetrix, Inc. Complexity management of genomic DNA
ES2256415T3 (es) 2001-07-31 2006-07-16 Pfizer Products Inc. Ensayos basados en celulas y secuencias para la fosfodiesterasa 10a.
US6696271B2 (en) 2001-08-23 2004-02-24 The Regents Of The University Of California Frozen tissue microarray technology for analysis of RNA, DNA, and proteins
EP1451348B1 (en) 2001-09-10 2015-08-12 Meso Scale Technologies, LLC Methods, reagents, kits and apparatus for protein function analysis
US20060188875A1 (en) 2001-09-18 2006-08-24 Perlegen Sciences, Inc. Human genomic polymorphisms
US20040019005A1 (en) 2001-10-01 2004-01-29 Jeffrey Van Ness Methods for parallel measurement of genetic variations
WO2003031591A2 (en) 2001-10-10 2003-04-17 Superarray Bioscience Corporation Detecting targets by unique identifier nucleotide tags
US6942972B2 (en) 2001-10-24 2005-09-13 Beckman Coulter, Inc. Efficient synthesis of protein-oligonucleotide conjugates
US20030175947A1 (en) 2001-11-05 2003-09-18 Liu Robin Hui Enhanced mixing in microfluidic devices
AU2002365271B2 (en) 2001-11-06 2008-12-11 Agilix Corporation Sensitive coded detection systems
JP2005535283A (ja) 2001-11-13 2005-11-24 ルビコン ゲノミクス インコーポレイテッド ランダムフラグメント化により生成されたdna分子を用いたdna増幅および配列決定
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
AU2002359508A1 (en) 2001-11-26 2003-06-10 Keck Graduate Institute Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US7098041B2 (en) 2001-12-11 2006-08-29 Kimberly-Clark Worldwide, Inc. Methods to view and analyze the results from diffraction-based diagnostics
AU2003232881A1 (en) 2002-01-16 2003-09-09 Mayo Foundation For Medical Education And Research Method and apparatus for image-guided therapy
US20040002090A1 (en) 2002-03-05 2004-01-01 Pascal Mayer Methods for detecting genome-wide sequence variations associated with a phenotype
US20030170637A1 (en) 2002-03-06 2003-09-11 Pirrung Michael C. Method of analyzing mRNA splice variants
US7223371B2 (en) 2002-03-14 2007-05-29 Micronics, Inc. Microfluidic channel network device
WO2003088904A2 (en) 2002-04-15 2003-10-30 The Regents Of The University Of California Screening and therapeutic methods for treating circadian rhythm disorders
WO2003089454A2 (en) 2002-04-19 2003-10-30 California Institute Of Technology Unnatural amino acid containing display libraries
DK2260875T3 (da) 2002-05-06 2014-06-30 Endocyte Inc Folatreceptor-targetede billeddannelsesmidler
ATE469135T1 (de) 2002-05-30 2010-06-15 Scripps Research Inst Kupferkatalysierte ligierung von aziden und acetylenen
US7108976B2 (en) 2002-06-17 2006-09-19 Affymetrix, Inc. Complexity management of genomic DNA by locus specific amplification
AU2003253661A1 (en) 2002-06-18 2003-12-31 Invitrogen Corp Methods and apparatus for low resistance electrophoresis of prior-cast, hydratable separation media
FR2841063B1 (fr) 2002-06-18 2004-09-17 Commissariat Energie Atomique Dispositif de deplacement de petits volumes de liquide le long d'un micro-catenaire par des forces electrostatiques
US20050019776A1 (en) 2002-06-28 2005-01-27 Callow Matthew James Universal selective genome amplification and universal genotyping system
US7205128B2 (en) 2002-08-16 2007-04-17 Agilent Technologies, Inc. Method for synthesis of the second strand of cDNA
JP2006509040A (ja) 2002-08-23 2006-03-16 ソレックサ リミテッド 修飾されたヌクレオチド
US20070166725A1 (en) 2006-01-18 2007-07-19 The Regents Of The University Of California Multiplexed diagnostic platform for point-of care pathogen detection
US20040038385A1 (en) 2002-08-26 2004-02-26 Langlois Richard G. System for autonomous monitoring of bioagents
CA2498362C (en) 2002-09-11 2012-11-06 Temple University - Of The Commonwealth System Of Higher Education Automated system for high-throughput electrophoretic separations
US7595883B1 (en) 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
DE60324810D1 (de) 2002-09-20 2009-01-02 New England Biolabs Inc HELICASE-ABHuNGIGE AMPLIFIKATION VON NUKLEINSUREN
US6911132B2 (en) 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
WO2004028955A2 (en) 2002-09-25 2004-04-08 California Institute Of Technology Microfluidic large scale integration
EP1578432A4 (en) 2002-10-03 2008-07-30 Epimmune Inc HLA BINDING PEPTIDES AND USES THEREOF
US20040259105A1 (en) 2002-10-03 2004-12-23 Jian-Bing Fan Multiplex nucleic acid analysis using archived or fixed samples
US20040067492A1 (en) 2002-10-04 2004-04-08 Allan Peng Reverse transcription on microarrays
ES2372169T3 (es) 2002-10-31 2012-01-16 University Of Massachusetts Método y aparato de empotramiento rápido de bloques con células.
WO2004055159A2 (en) 2002-12-12 2004-07-01 Chiron Corporation Identification of oligonucleotides for the capture, detection and quantitation of west nile virus
AU2003301061A1 (en) 2002-12-18 2004-07-22 West Virginia University Research Corporation Apparatus and method for edman degradation using a microfluidic system
KR20040062847A (ko) 2003-01-03 2004-07-09 삼성전자주식회사 핵산 어레이의 복제방법
US7547380B2 (en) 2003-01-13 2009-06-16 North Carolina State University Droplet transportation devices and methods having a fluid surface
GB0302058D0 (en) 2003-01-29 2003-02-26 Univ Cranfield Replication of nucleic acid arrays
EP1594980B2 (en) 2003-01-29 2017-08-16 454 Life Sciences Corporation Bead emulsion nucleic acid amplification
FR2852317B1 (fr) 2003-03-13 2006-08-04 Biopuces a sondes et leurs methodes d'utilisation
US7083980B2 (en) 2003-04-17 2006-08-01 Wisconsin Alumni Research Foundation Tn5 transposase mutants and the use thereof
US7267994B2 (en) 2003-04-28 2007-09-11 Regents Of The University Of California Element-coded affinity tags
US7666612B2 (en) 2003-05-23 2010-02-23 Epfl-Ecole Polytechnique Federale De Lausanne Methods for protein labeling based on acyl carrier protein
EP1628753A1 (en) 2003-05-30 2006-03-01 Applera Corporation Apparatus and method for hybridization and spr detection
US20050026188A1 (en) 2003-05-30 2005-02-03 Van Kessel Andrew G. Methods of identifying, characterizing and comparing organism communities
WO2005047543A2 (en) 2003-06-10 2005-05-26 Applera Corporation Ligation assay
US20060216775A1 (en) 2003-06-17 2006-09-28 The Regents Of The University Of Califoenia Compositions and methods for analysis and manipulation of enzymes in biosynthetic proteomes
JP2007525963A (ja) 2003-06-20 2007-09-13 イルミナ インコーポレイテッド 全ゲノム増幅および遺伝型決定のための方法および組成物
US20070128656A1 (en) 2003-06-26 2007-06-07 University Of South Florida Direct Fluorescent Label Incorporation Via 1st Strand cDNA Synthesis
US7601492B2 (en) 2003-07-03 2009-10-13 The Regents Of The University Of California Genome mapping of functional DNA elements and cellular proteins
EP2918595B1 (en) 2003-07-05 2019-12-11 The Johns-Hopkins University Method and compositions for detection and enumeration of genetic variations
EP1660858A4 (en) 2003-07-21 2007-10-24 Amplified Proteomics Inc MULTIPLEX analyte
US20050031176A1 (en) 2003-08-08 2005-02-10 Hertel Sarah R. Method and apparatus of multi-modality image fusion
EP1651779A4 (en) 2003-08-08 2006-10-04 Univ Jefferson METHOD FOR FAST IDENTIFICATION OF ALTERNATIVE SPLEISSEN
US20050037362A1 (en) 2003-08-11 2005-02-17 Eppendorf Array Technologies, S.A. Detection and quantification of siRNA on microarrays
DK1664345T3 (da) 2003-09-02 2015-06-22 Keygene Nv OLA-baserede fremgangsmåder til detektion af target-nukleinsyresekvenser
EP1660674B1 (en) 2003-09-10 2010-03-17 Althea Technologies, Inc. Expression profiling using microarrays
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
DK1670939T3 (da) 2003-09-18 2010-03-01 Nuevolution As Fremgangsmåde til opnåelse af strukturel information om et kodet molekyle og fremgangsmåde til udvælgelse af forbindelser
US7541166B2 (en) 2003-09-19 2009-06-02 Microfluidic Systems, Inc. Sonication to selectively lyse different cell types
US20050064435A1 (en) 2003-09-24 2005-03-24 Xing Su Programmable molecular barcodes
ATE434131T1 (de) 2003-11-17 2009-07-15 Koninkl Philips Electronics Nv System zur handhabung einer fluidmenge
WO2005051984A2 (en) 2003-11-21 2005-06-09 Arena Pharmaceuticals, Inc. Methods for producing olfactory gpcrs
JP2007525661A (ja) 2003-12-12 2007-09-06 セントルイス ユニバーシティー 大分子その他の分析物の検出用生物センサー
US7259258B2 (en) 2003-12-17 2007-08-21 Illumina, Inc. Methods of attaching biological compounds to solid supports using triazine
US20050132463A1 (en) 2003-12-19 2005-06-23 Kimberly-Clark Worldwide, Inc. Surgical gown having adhesive tabs and methods of use
US20050136414A1 (en) 2003-12-23 2005-06-23 Kevin Gunderson Methods and compositions for making locus-specific arrays
WO2005089164A2 (en) 2003-12-31 2005-09-29 Pharmexa Inc. Inducing cellular immune responses to human papillomavirus using peptide and nucleic acid compositions
EP1701785A1 (en) 2004-01-07 2006-09-20 Solexa Ltd. Modified molecular arrays
WO2005067648A2 (en) 2004-01-08 2005-07-28 Vanderbilt University Multiplex spatial profiling of gene expression
US7569392B2 (en) 2004-01-08 2009-08-04 Vanderbilt University Multiplex spatial profiling of gene expression
EP1709203A2 (en) 2004-01-23 2006-10-11 Lingvitae AS Improving polynucleotide ligation reactions
FR2866493B1 (fr) 2004-02-16 2010-08-20 Commissariat Energie Atomique Dispositif de controle du deplacement d'une goutte entre deux ou plusieurs substrats solides
WO2005089508A2 (en) 2004-03-18 2005-09-29 Atom Sciences, Inc. Dna sequence detection by limited primer extension
FR2868638B1 (fr) 2004-03-30 2006-05-19 Sagem Procede d'echange d'informations entre deux reseaux fonctionnant sous des protocoles de routages differents
KR100624420B1 (ko) 2004-04-10 2006-09-19 삼성전자주식회사 마이크로어레이에 대한 정보가 스팟의 형태로 저장되어있는 마이크로어레이, 그를 제조하는 방법 및 그를이용하는 방법
US20050260653A1 (en) 2004-04-14 2005-11-24 Joshua Labaer Nucleic-acid programmable protein arrays
RU2270254C2 (ru) 2004-04-30 2006-02-20 Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук Способ идентификации трансгенных последовательностей днк в растительном материале и продуктах на его основе, набор олигонуклеотидов и биочип для осуществления этого способа
DE102004022263A1 (de) * 2004-05-06 2005-12-15 Clondiag Chip Technologies Gmbh Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen
US7618780B2 (en) 2004-05-20 2009-11-17 Trillion Genomics Limited Use of mass labelled probes to detect target nucleic acids using mass spectrometry
US7906276B2 (en) 2004-06-30 2011-03-15 Kimberly-Clark Worldwide, Inc. Enzymatic detection techniques
FR2872715B1 (fr) 2004-07-08 2006-11-17 Commissariat Energie Atomique Microreacteur goutte
FR2872809B1 (fr) 2004-07-09 2006-09-15 Commissariat Energie Atomique Methode d'adressage d'electrodes
US7608434B2 (en) 2004-08-04 2009-10-27 Wisconsin Alumni Research Foundation Mutated Tn5 transposase proteins and the use thereof
WO2006073504A2 (en) 2004-08-04 2006-07-13 President And Fellows Of Harvard College Wobble sequencing
JP2006058031A (ja) 2004-08-17 2006-03-02 Hitachi High-Technologies Corp 化学分析装置
US7776547B2 (en) * 2004-08-26 2010-08-17 Intel Corporation Cellular analysis using Raman surface scanning
US20060228758A1 (en) 2004-09-13 2006-10-12 Xencor, Inc. Analysis of MHC-peptide binding interactions
US20060062531A1 (en) 2004-09-17 2006-03-23 Stephen Turner Fabrication of optical confinements
JP4318721B2 (ja) 2004-10-15 2009-08-26 独立行政法人科学技術振興機構 mRNA−ピューロマイシン−タンパク質連結体作製用リンカー
ES2534302T3 (es) 2004-11-12 2015-04-21 Asuragen, Inc. Procedimientos y composiciones que implican miARN y moléculas inhibidoras de miARN
US7183119B2 (en) * 2004-11-15 2007-02-27 Eastman Kodak Company Method for sensitive detection of multiple biological analytes
US7745143B2 (en) 2004-11-19 2010-06-29 Plexera, Llc Plasmon resonance biosensor and method
EP1828412B2 (en) 2004-12-13 2019-01-09 Illumina Cambridge Limited Improved method of nucleotide detection
GB0427236D0 (en) 2004-12-13 2005-01-12 Solexa Ltd Improved method of nucleotide detection
US20060292586A1 (en) 2004-12-17 2006-12-28 Schroth Gary P ID-tag complexes, arrays, and methods of use thereof
JP2008526877A (ja) 2005-01-05 2008-07-24 エージェンコート パーソナル ジェノミクス 可逆的ヌクレオチドターミネーターおよびその使用
US7458661B2 (en) 2005-01-25 2008-12-02 The Regents Of The University Of California Method and apparatus for promoting the complete transfer of liquid drops from a nozzle
WO2006081222A2 (en) 2005-01-25 2006-08-03 Compass Genetics, Llc. Isothermal dna amplification
DK1859330T3 (da) 2005-01-28 2012-10-15 Univ Duke Apparater og fremgangsmåder til håndtering af små dråber på et trykt kredsløbskort
AU2006210551A1 (en) 2005-02-03 2006-08-10 Perkinelmer Las, Inc. Ultra-sensitive detection systems using multidimension signals
US7407757B2 (en) 2005-02-10 2008-08-05 Population Genetics Technologies Genetic analysis by sequence-specific sorting
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
US20060199207A1 (en) 2005-02-24 2006-09-07 Matysiak Stefan M Self-assembly of molecules using combinatorial hybridization
US7727721B2 (en) 2005-03-08 2010-06-01 California Institute Of Technology Hybridization chain reaction amplification for in situ imaging
US7776567B2 (en) 2005-03-17 2010-08-17 Biotium, Inc. Dimeric and trimeric nucleic acid dyes, and associated systems and methods
US7601498B2 (en) 2005-03-17 2009-10-13 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection and associated technology
US7303880B2 (en) 2005-03-18 2007-12-04 Wisconsin Alumni Research Foundation Microdissection-based methods for determining genomic features of single chromosomes
US7229769B2 (en) 2005-03-25 2007-06-12 Illumina, Inc. Compositions and methods for detecting protease activity
ES2313143T3 (es) 2005-04-06 2009-03-01 Maurice Stroun Metodo para el diagnostico de cancer mediante la deteccion de adn y arn circulantes.
GB0508983D0 (en) 2005-05-03 2005-06-08 Oxford Gene Tech Ip Ltd Cell analyser
JP4990886B2 (ja) 2005-05-10 2012-08-01 ソレックサ リミテッド 改良ポリメラーゼ
WO2006124644A2 (en) 2005-05-12 2006-11-23 Board Of Regents, The University Of Texas System Protein and antibody profiling using small molecule microarrays
CA2607221A1 (en) 2005-05-12 2006-11-23 Panomics, Inc. Multiplex branched-chain dna assays
WO2006125143A2 (en) 2005-05-18 2006-11-23 Novartis Ag Methods for diagnosis and treatment of proliferative disorders mediated by cd40 signaling
US20060263789A1 (en) 2005-05-19 2006-11-23 Robert Kincaid Unique identifiers for indicating properties associated with entities to which they are attached, and methods for using
CN100526453C (zh) 2005-05-20 2009-08-12 麦克奥迪实业集团有限公司 激光显微切割后细胞收集方法
US20090305237A1 (en) 2005-05-26 2009-12-10 Trustees Of Boston University Quantification of nucleic acids and proteins using oligonucleotide mass tags
US8486629B2 (en) 2005-06-01 2013-07-16 Bioarray Solutions, Ltd. Creation of functionalized microparticle libraries by oligonucleotide ligation or elongation
EP1910537A1 (en) 2005-06-06 2008-04-16 454 Life Sciences Corporation Paired end sequencing
EP2322920B1 (en) 2005-06-07 2014-12-10 Centre National de la Recherche Scientifique (CNRS) MALDI tissue imaging using conjugates cleavable by fast fragmentation
GB0511717D0 (en) 2005-06-09 2005-07-13 Babraham Inst Repeatable protein arrays
EP1907571B1 (en) 2005-06-15 2017-04-26 Complete Genomics Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
US20090264299A1 (en) 2006-02-24 2009-10-22 Complete Genomics, Inc. High throughput genome sequencing on DNA arrays
CN101495650B (zh) 2005-06-20 2015-02-04 领先细胞医疗诊断有限公司 检测单个细胞中的核酸和鉴定异质大细胞群中罕见细胞的方法
US7873193B2 (en) 2005-06-21 2011-01-18 Carl Zeiss Microimaging Gmbh Serial section analysis for computer-controlled microscopic imaging
DK2292788T3 (da) 2005-06-23 2012-07-23 Keygene Nv Strategier til identifikation og detektion af polymorfismer med højt gennemløb
WO2007005649A2 (en) 2005-06-30 2007-01-11 Applera Corporation Proximity probing of target proteins comprising restriction and/or extension field
US7883848B2 (en) 2005-07-08 2011-02-08 Olink Ab Regulation analysis by cis reactivity, RACR
JP4822753B2 (ja) 2005-07-11 2011-11-24 一般社団法人オンチップ・セロミクス・コンソーシアム 細胞構成物質分取チップならびに細胞構成物質解析システム及びこれらを用いる細胞構成物質解析法
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US20070020640A1 (en) 2005-07-21 2007-01-25 Mccloskey Megan L Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis
US20070023292A1 (en) 2005-07-26 2007-02-01 The Regents Of The University Of California Small object moving on printed circuit board
US7805081B2 (en) 2005-08-11 2010-09-28 Pacific Biosciences Of California, Inc. Methods and systems for monitoring multiple optical signals from a single source
WO2007027653A1 (en) 2005-09-01 2007-03-08 Promega Corporation Cell-based luminogenic and nonluminogenic proteasome assays
WO2007030373A2 (en) 2005-09-07 2007-03-15 St. Jude Children's Research Hospital Method for in situ hybridization analysis
JP2007074967A (ja) 2005-09-13 2007-03-29 Canon Inc 識別子プローブ及びそれを用いた核酸増幅方法
JP5237099B2 (ja) 2005-09-29 2013-07-17 キージーン ナムローゼ フェンノートシャップ 変異させた集団のハイスループットスクリーニング
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
WO2007041689A2 (en) 2005-10-04 2007-04-12 President And Fellows Of Harvard College Methods of site-specific labeling of molecules and molecules produced thereby
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
US20070116612A1 (en) 2005-11-02 2007-05-24 Biopath Automation, L.L.C. Prefix tissue cassette
WO2007120208A2 (en) 2005-11-14 2007-10-25 President And Fellows Of Harvard College Nanogrid rolling circle dna sequencing
JP2009517651A (ja) 2005-11-25 2009-04-30 コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ 酵素活性を決定するための磁気バイオセンサ
WO2007100392A2 (en) 2005-11-30 2007-09-07 Biotium, Inc. Enzyme substrate comprising a functional dye and associated technology and methods
US20070161029A1 (en) 2005-12-05 2007-07-12 Panomics, Inc. High throughput profiling of methylation status of promoter regions of genes
US7803751B2 (en) 2005-12-09 2010-09-28 Illumina, Inc. Compositions and methods for detecting phosphomonoester
US20070178503A1 (en) 2005-12-19 2007-08-02 Feng Jiang In-situ genomic DNA chip for detection of cancer
US20070141718A1 (en) 2005-12-19 2007-06-21 Bui Huy A Reduction of scan time in imaging mass spectrometry
EP2363504A1 (en) 2005-12-22 2011-09-07 Keygene N.V. Method for high-throughtput AFLP-based polymorphism detection
EP1966394B1 (en) 2005-12-22 2012-07-25 Keygene N.V. Improved strategies for transcript profiling using high throughput sequencing technologies
CA2640385C (en) 2005-12-23 2014-07-15 Nanostring Technologies, Inc. Nanoreporters and methods of manufacturing and use thereof
CN101346971B (zh) 2005-12-23 2011-05-18 艾利森电话股份有限公司 用于解决数据分组业务拥塞的方法和设备
WO2007076726A1 (en) 2006-01-04 2007-07-12 Si Lok Methods for nucleic acid mapping and identification of fine-structural-variations in nucleic acids and utilities
EP1987162A4 (en) 2006-01-23 2009-11-25 Population Genetics Technologi NUCLEIC ACID ANALYSIS ABOUT SEQUENCE TOKENS
WO2007087312A2 (en) * 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
US20070251824A1 (en) 2006-01-24 2007-11-01 Perkinelmer Las, Inc. Multiplexed analyte quantitation by two-dimensional planar electrochromatography
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
JP5180845B2 (ja) 2006-02-24 2013-04-10 カリダ・ジェノミックス・インコーポレイテッド Dnaアレイ上でのハイスループットゲノム配列決定
JP5237126B2 (ja) 2006-03-01 2013-07-17 キージーン ナムローゼ フェンノートシャップ ライゲーションアッセイを用いてハイスループットシークエンスに基づき遺伝子関連配列を検出する方法
US20080009420A1 (en) 2006-03-17 2008-01-10 Schroth Gary P Isothermal methods for creating clonal single molecule arrays
US20070231823A1 (en) * 2006-03-23 2007-10-04 Mckernan Kevin J Directed enrichment of genomic DNA for high-throughput sequencing
US8975216B2 (en) 2006-03-30 2015-03-10 Pacific Biosciences Of California Articles having localized molecules disposed thereon and methods of producing same
EP2018622B1 (en) 2006-03-31 2018-04-25 Illumina, Inc. Systems for sequence by synthesis analysis
DK3239304T3 (da) 2006-04-04 2020-10-26 Keygene Nv Højgennemløbsdetektering af molekylære markører baseret på AFLP og sekventering med højt gennemløb
CA2680061C (en) 2006-04-18 2015-10-13 Duke University Droplet-based biochemistry
US8383338B2 (en) 2006-04-24 2013-02-26 Roche Nimblegen, Inc. Methods and systems for uniform enrichment of genomic regions
WO2007127458A2 (en) 2006-04-28 2007-11-08 Nsabp Foundation, Inc. Methods of whole genome or microarray expression profiling using nucleic acids
WO2007133703A2 (en) 2006-05-10 2007-11-22 Dxterity Diagnostics Detection of nucleic acid targets using chemically reactive oligonucleotide probes
JP2007304043A (ja) 2006-05-15 2007-11-22 Canon Inc プローブ固定担体の製造方法
AU2007268027B2 (en) 2006-05-22 2012-08-09 Bruker Spatial Biology, Inc. Systems and methods for analyzing nanoreporters
US20080132429A1 (en) 2006-05-23 2008-06-05 Uchicago Argonne Biological microarrays with enhanced signal yield
CA2653408C (en) 2006-05-27 2017-08-01 Mitchell A. Winnik Polymer backbone element tags
WO2008002502A2 (en) 2006-06-23 2008-01-03 Illumina, Inc. Devices and systems for creation of dna cluster arrays
US20090253582A1 (en) 2006-06-29 2009-10-08 Ge Healthcare Bio-Sciences Ab Chamber apparatus
US8178316B2 (en) 2006-06-29 2012-05-15 President And Fellows Of Harvard College Evaluating proteins
AT503862B1 (de) 2006-07-05 2010-11-15 Arc Austrian Res Centers Gmbh Pathogen-identifizierung anhand eines 16s oder 18s-rrna mikroarray
JP4881950B2 (ja) 2006-07-10 2012-02-22 株式会社日立ハイテクノロジーズ 液体搬送デバイス
US9012390B2 (en) 2006-08-07 2015-04-21 Raindance Technologies, Inc. Fluorocarbon emulsion stabilizing surfactants
WO2008022332A2 (en) 2006-08-18 2008-02-21 Board Of Regents, The University Of Texas System System, method and kit for replicating a dna array
US20080047835A1 (en) 2006-08-22 2008-02-28 Macconnell William P Genomic DNA Purifier
EP3936857B1 (en) 2006-09-01 2023-06-21 Pacific Biosciences Of California, Inc. Substrates, systems and methods for analyzing materials
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
AU2007309504B2 (en) 2006-10-23 2012-09-13 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US9266076B2 (en) 2006-11-02 2016-02-23 The Regents Of The University Of California Method and apparatus for real-time feedback control of electrical manipulation of droplets on chip
US20080108804A1 (en) 2006-11-02 2008-05-08 Kabushiki Kaisha Dnaform Method for modifying RNAS and preparing DNAS from RNAS
US20110045462A1 (en) 2006-11-14 2011-02-24 The Regents Of The University Of California Digital analysis of gene expression
US9201063B2 (en) 2006-11-16 2015-12-01 General Electric Company Sequential analysis of biological samples
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
EP2677309B9 (en) 2006-12-14 2014-11-19 Life Technologies Corporation Methods for sequencing a nucleic acid using large scale FET arrays, configured to measure a limited pH range
CN101221182A (zh) 2007-01-08 2008-07-16 山东司马特生物芯片有限公司 一种荧光蛋白质芯片血清肿瘤系列诊断新方法
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US20080220434A1 (en) 2007-02-07 2008-09-11 Perscitus Biosciences, Llc Detection Of Molecule Proximity
AU2008216303A1 (en) 2007-02-12 2008-08-21 Proteonova, Inc. Generation of library of soluble random polypeptides linked to mRNA
KR100819006B1 (ko) 2007-02-13 2008-04-03 삼성전자주식회사 마이크로 어레이용 마스크 세트, 이의 제조 방법, 및마스크 세트를 이용한 마이크로 어레이의 제조 방법
US8148518B2 (en) 2007-02-14 2012-04-03 Eastman Chemical Company Cellulose esters and their production in carboxylated ionic liquids
KR101017808B1 (ko) 2007-04-04 2011-02-28 엔에이치엔(주) 편집 파일 자동 생성 방법 및 그 장치
US20090006002A1 (en) 2007-04-13 2009-01-01 Sequenom, Inc. Comparative sequence analysis processes and systems
AU2008256851A1 (en) 2007-05-23 2008-12-04 Oregon Health & Science University Microarray systems and methods for identifying DNA-binding proteins
EP2164985A4 (en) 2007-06-01 2014-05-14 454 Life Sciences Corp SYSTEM AND METHOD FOR IDENTIFYING INDIVIDUAL SAMPLES FROM A MULTIPLEX MIXTURE
US8926811B2 (en) 2007-06-27 2015-01-06 Digital Biosystems Digital microfluidics based apparatus for heat-exchanging chemical processes
EP2395113A1 (en) 2007-06-29 2011-12-14 Population Genetics Technologies Ltd. Methods and compositions for isolating nucleic acid sequence variants
JP2009025385A (ja) 2007-07-17 2009-02-05 Nitto Denko Corp フィルム状光導波路の製造方法
US12180549B2 (en) 2007-07-23 2024-12-31 The Chinese University Of Hong Kong Diagnosing fetal chromosomal aneuploidy using genomic sequencing
CN101952719A (zh) 2007-07-27 2011-01-19 通信改革公司 检测测定法及其用途
JP2009036694A (ja) 2007-08-03 2009-02-19 Tokyo Medical & Dental Univ 空間分布を保った細胞内生体物質の解析方法
WO2009032167A1 (en) 2007-08-29 2009-03-12 Illumina Cambridge Method for sequencing a polynucleotide template
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
CA2697640C (en) 2007-09-21 2016-06-21 Katholieke Universiteit Leuven Tools and methods for genetic tests using next generation sequencing
EP2053132A1 (en) 2007-10-23 2009-04-29 Roche Diagnostics GmbH Enrichment and sequence analysis of geomic regions
US8518640B2 (en) 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
KR20090081260A (ko) 2008-01-23 2009-07-28 삼성전자주식회사 마이크로 어레이 혼성화 검출 방법
DE102008014687A1 (de) 2008-03-18 2009-09-24 Smartrac Ip B.V. Lagenverbund für einen Kartenkörper und Verfahren zur Herstellung des Lagenverbunds
WO2009117698A2 (en) 2008-03-21 2009-09-24 Nugen Technologies, Inc. Methods of rna amplification in the presence of dna
US20090253163A1 (en) 2008-04-02 2009-10-08 General Electric Company Iterative staining of biological samples
DE102008023438B4 (de) 2008-05-14 2011-06-30 Bruker Daltonik GmbH, 28359 Verfahren zur Analyse von Gewebeschnitten
US8093064B2 (en) 2008-05-15 2012-01-10 The Regents Of The University Of California Method for using magnetic particles in droplet microfluidics
DE102008025656B4 (de) 2008-05-28 2016-07-28 Genxpro Gmbh Verfahren zur quantitativen Analyse von Nukleinsäuren, Marker dafür und deren Verwendung
WO2009148560A2 (en) 2008-05-30 2009-12-10 Board Of Regents, The Universtiy Of Texas System Methods and compositions for nucleic acid sequencing
GB0811574D0 (en) 2008-06-24 2008-07-30 Trillion Genomics Ltd Characterising planar samples by mass spectrometry
EP2291533B2 (en) 2008-07-02 2020-09-30 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces
US20100035249A1 (en) 2008-08-05 2010-02-11 Kabushiki Kaisha Dnaform Rna sequencing and analysis using solid support
WO2010019826A1 (en) * 2008-08-14 2010-02-18 Nanostring Technologies, Inc Stable nanoreporters
US8697363B2 (en) 2008-08-26 2014-04-15 Fluidigm Corporation Methods for detecting multiple target nucleic acids in multiple samples by use nucleotide tags
EP2163900A1 (en) 2008-09-04 2010-03-17 Commissariat A L'energie Atomique New method of imaging by mass spectrometry and new mass tag associated trityl derivatives
US20100055733A1 (en) 2008-09-04 2010-03-04 Lutolf Matthias P Manufacture and uses of reactive microcontact printing of biomolecules on soft hydrogels
US8586310B2 (en) 2008-09-05 2013-11-19 Washington University Method for multiplexed nucleic acid patch polymerase chain reaction
WO2010026950A1 (ja) 2008-09-08 2010-03-11 株式会社日立製作所 Dna検出装置、及びdna検出デバイス、並びにdna検出方法
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
WO2010033844A2 (en) 2008-09-22 2010-03-25 Biomicro, Inc. Selective processing of biological material on a microarray substrate
CN102227440A (zh) 2008-09-30 2011-10-26 雅培制药有限公司 改良的抗体文库
US8361298B2 (en) 2008-10-08 2013-01-29 Sage Science, Inc. Multichannel preparative electrophoresis system
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
WO2010062310A1 (en) 2008-10-28 2010-06-03 Millipore Corporation Biological culture assembly
EP2719774B8 (en) 2008-11-07 2020-04-22 Adaptive Biotechnologies Corporation Methods of monitoring conditions by sequence analysis
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
WO2010081114A2 (en) 2009-01-12 2010-07-15 20/20 Genesystems, Inc. Oligonucleotide-coated affinity membranes and uses thereof
KR101059565B1 (ko) 2009-02-11 2011-08-26 어플라이드 프레시젼, 인코포레이티드 밝은 기준점 표지를 갖는 마이크로어레이 및 그로부터 광 데이터를 수집하는 방법
CN102625850B (zh) 2009-04-03 2014-11-26 蒂莫西·Z·刘 多重核酸检测方法和系统
WO2010126614A2 (en) 2009-04-30 2010-11-04 Good Start Genetics, Inc. Methods and compositions for evaluating genetic markers
WO2010127186A1 (en) 2009-04-30 2010-11-04 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
RU2410439C1 (ru) 2009-07-06 2011-01-27 Российская Федерация, От Имени Которой Выступает Министерство Образования И Науки Российской Федерации Способ абляции целевой днк с поверхности днк-биочипов
WO2011008831A2 (en) 2009-07-14 2011-01-20 University Of Florida Research Foundation, Inc. Mass tags for spectrometric analysis of immunoglobulins
KR101029343B1 (ko) 2009-07-30 2011-04-13 한국과학기술연구원 면역분석 기반의 항원 검출용 키트 및 항원 검출 방법
AU2010278710A1 (en) 2009-07-31 2012-03-15 Prognosys Biosciences, Inc. Assay tools and methods of use
US9416409B2 (en) 2009-07-31 2016-08-16 Ibis Biosciences, Inc. Capture primers and capture sequence linked solid supports for molecular diagnostic tests
EP3029141A1 (en) 2009-08-20 2016-06-08 Population Genetics Technologies Ltd. Compositions and methods for intramolecular nucleic acid rearrangement
US8496798B2 (en) 2009-09-01 2013-07-30 Oregon Health & Science University Reversible current gel electrophoresis device for separating biological macromolecules
SG169918A1 (en) 2009-10-02 2011-04-29 Fluidigm Corp Microfluidic devices with removable cover and methods of fabrication and application
ES2461992T3 (es) 2009-10-09 2014-05-22 Invisible Sentinel, Inc. Dispositivo para la detección de antígenos y usos de los mismos
EP3236264A3 (en) 2009-10-13 2017-11-08 Nanostring Technologies, Inc Protein detection via nanoreporters
US20120277113A1 (en) 2009-11-18 2012-11-01 Ruo-Pan Huang Array-based proximity ligation association assays
JP5415560B2 (ja) 2009-12-04 2014-02-12 株式会社日立製作所 2次元cDNAライブラリを用いた遺伝子発現解析方法
SG10201407883PA (en) 2009-12-07 2015-01-29 Illumina Inc Multi-sample indexing for multiplex genotyping
CN102648294A (zh) 2009-12-07 2012-08-22 普罗格诺西斯生物科学公司 肽展示阵列
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
EP2529030B1 (en) 2010-01-29 2019-03-13 Advanced Cell Diagnostics, Inc. Methods of in situ detection of nucleic acids
EP2354242A1 (en) 2010-02-03 2011-08-10 Epiontis GmbH Assay for determining the type and/or status of a cell based on the epigenetic pattern and the chromatin structure
EP2516681B1 (en) 2010-02-11 2017-10-18 Nanostring Technologies, Inc Compositions and methods for the detection of preferably small rnas by bridge hybridisation and ligation
EP2536818B1 (en) 2010-02-18 2018-10-03 Bima Limited Immobilised-bead immunomultiplex assay
JP5665021B2 (ja) 2010-03-08 2015-02-04 国立大学法人東京農工大学 融合mhc分子連結磁気微粒子、抗原ペプチドのスクリーニング方法、組換えベクター、及び磁性細菌の形質転換体
US10267808B2 (en) 2010-03-08 2019-04-23 California Institute Of Technology Molecular indicia of cellular constituents and resolving the same by super-resolution technologies in single cells
WO2011127006A1 (en) 2010-04-05 2011-10-13 Prognosys Biosciences, Inc. Co-localization affinity assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
RS54482B1 (sr) 2010-04-05 2016-06-30 Prognosys Biosciences, Inc. Biološka testiranja sa prostornim kodiranjem
US20130059741A1 (en) 2010-05-13 2013-03-07 Illumina, Inc. Binding assays for markers
EP3425062B1 (en) 2010-06-09 2023-08-02 Keygene N.V. Combinatorial sequence barcodes for high throughput screening
ES2676183T3 (es) 2010-07-02 2018-07-17 Ventana Medical Systems, Inc. Detección de dianas usando marcas de masa y espectrometría de masas
WO2012012772A2 (en) 2010-07-22 2012-01-26 The Johns Hopkins University Drug eluting hydrogels for catheter delivery
GB201013767D0 (en) 2010-08-17 2010-09-29 Isis Innovation Identification of ligands and their use
ES2690753T3 (es) 2010-09-21 2018-11-22 Agilent Technologies, Inc. Aumento de la confianza en las identificaciones de alelos con el recuento molecular
IN2013MN00522A (OSRAM) 2010-09-24 2015-05-29 Univ Leland Stanford Junior
US9110079B2 (en) 2010-09-29 2015-08-18 Biomerieux Method and kit for establishing an in vitro prognosis on a patient exhibiting SIRS
EP2625320B1 (en) 2010-10-08 2019-03-27 President and Fellows of Harvard College High-throughput single cell barcoding
WO2012049316A1 (en) 2010-10-15 2012-04-19 Olink Ab Dynamic range methods
EP4328321A3 (en) 2010-10-22 2024-05-29 Cold Spring Harbor Laboratory Varietal counting of nucleic acids for obtaining genomic copy number information
US9096899B2 (en) 2010-10-27 2015-08-04 Illumina, Inc. Microdevices and biosensor cartridges for biological or chemical analysis and systems and methods for the same
EP2633080B1 (en) 2010-10-29 2018-12-05 President and Fellows of Harvard College Method of detecting targets using fluorescently labelled nucleic acid nanotube probes
US20130035259A1 (en) 2010-11-22 2013-02-07 The University Of Chicago Methods and/or Systems Producing and Providing Sets of Oligonucleotide Conjugates for Assays and Detections
US20140121118A1 (en) 2010-11-23 2014-05-01 Opx Biotechnologies, Inc. Methods, systems and compositions regarding multiplex construction protein amino-acid substitutions and identification of sequence-activity relationships, to provide gene replacement such as with tagged mutant genes, such as via efficient homologous recombination
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
JP6054303B2 (ja) 2010-12-30 2016-12-27 ファウンデーション メディシン インコーポレイテッドFoundation Medicine, Inc. 腫瘍試料の多重遺伝子分析の最適化
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
SG192108A1 (en) 2011-01-25 2013-08-30 Almac Diagnostics Ltd Colon cancer gene expression signatures and methods of use
WO2012110899A2 (en) 2011-02-15 2012-08-23 Bernitz Mats Nilsson Method for localizedin situdetection of mrna
WO2012129363A2 (en) 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
US20120258871A1 (en) 2011-04-08 2012-10-11 Prognosys Biosciences, Inc. Peptide constructs and assay systems
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
CN110016499B (zh) 2011-04-15 2023-11-14 约翰·霍普金斯大学 安全测序系统
WO2012151111A1 (en) 2011-05-04 2012-11-08 Htg Molecular Diagnostics, Inc. Quantitative nuclease protection assay (qnpa) and sequencing (qnps) improvements
US8809238B2 (en) 2011-05-09 2014-08-19 Fluidigm Corporation Probe based nucleic acid detection
EP2718713B1 (en) 2011-06-06 2019-07-17 Koninklijke Philips N.V. Selective lysis of cells by ionic surfactants
CN107400711A (zh) 2011-08-03 2017-11-28 伯乐实验室公司 使用透化处理的细胞过滤小核酸
ES2639938T5 (es) 2011-09-23 2021-05-07 Illumina Inc Métodos y composiciones para la secuenciación de ácidos nucleicos
US8987174B2 (en) 2011-10-28 2015-03-24 Prognosys Biosciences, Inc. Methods for manufacturing molecular arrays
WO2013063382A2 (en) 2011-10-28 2013-05-02 Illumina, Inc. Microarray fabrication system and method
US8637242B2 (en) 2011-11-07 2014-01-28 Illumina, Inc. Integrated sequencing apparatuses and methods of use
DK2788499T3 (en) 2011-12-09 2016-03-21 Illumina Inc Enhanced root for polymer tags
WO2014163886A1 (en) 2013-03-12 2014-10-09 President And Fellows Of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
WO2013096838A2 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids
US9598728B2 (en) 2012-02-14 2017-03-21 Cornell University Method for relative quantification of nucleic acid sequence, expression, or copy changes, using combined nuclease, ligation, and polymerase reactions
SI3363901T1 (sl) 2012-02-17 2021-04-30 Fred Hutchinson Cancer Research Center Sestavki in postopki za natančno identifikacijo mutacij
US9862995B2 (en) 2012-03-13 2018-01-09 Abhijit Ajit Patel Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing
SG11201405669XA (en) 2012-03-13 2014-10-30 Swift Biosciences Inc Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase
WO2013142389A1 (en) 2012-03-20 2013-09-26 University Of Washington Through Its Center For Commercialization Methods of lowering the error rate of massively parallel dna sequencing using duplex consensus sequencing
FI2831279T3 (fi) 2012-03-26 2023-05-23 Univ Johns Hopkins Nopea aneuploidian havaitseminen
EP2647426A1 (en) 2012-04-03 2013-10-09 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Replication of distributed nucleic acid molecules with preservation of their relative distribution through hybridization-based binding
BR122021002358B8 (pt) 2012-04-03 2022-03-03 Illumina Inc Sistema fluídico
EP2659977B1 (en) 2012-05-02 2019-04-24 IMEC vzw Microfluidics system for sequencing
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
US20140378345A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
DK2904111T3 (en) 2012-10-01 2018-03-12 Adaptive Biotechnologies Corp Immune competence evaluation of adaptive immune receptor diversity and clonality characterization
USRE50065E1 (en) 2012-10-17 2024-07-30 10X Genomics Sweden Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
SG10202009015SA (en) 2012-12-10 2020-10-29 Resolution Bioscience Inc Methods for targeted genomic analysis
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
AU2013382089B2 (en) 2013-03-13 2018-05-10 Illumina, Inc. Multilayer fluidic devices and methods for their fabrication
CN105518151B (zh) 2013-03-15 2021-05-25 莱兰斯坦福初级大学评议会 循环核酸肿瘤标志物的鉴别和用途
US10266874B2 (en) 2013-06-12 2019-04-23 The General Hospital Corporation Methods, kits, and systems for multiplexed detection of target molecules and uses thereof
CN105849275B (zh) 2013-06-25 2020-03-17 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统
AU2014284584B2 (en) 2013-07-01 2019-08-01 Illumina, Inc. Catalyst-free surface functionalization and polymer grafting
WO2015054292A1 (en) 2013-10-07 2015-04-16 Cellular Research, Inc. Methods and systems for digitally counting features on arrays
US10408842B2 (en) 2014-05-30 2019-09-10 The Regents Of The University Of California Subcellular western blotting of single cells
EP3628684B1 (en) 2014-06-06 2024-09-18 Herlev Hospital Determining antigen recognition through barcoding of mhc multimers
EP3155426B1 (en) 2014-06-13 2023-07-19 Immudex ApS General detection and isolation of specific cells by binding of labeled molecules
CN113249435B (zh) 2014-06-26 2024-09-03 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
WO2016003814A1 (en) 2014-06-30 2016-01-07 Illumina, Inc. Methods and compositions using one-sided transposition
US10179932B2 (en) 2014-07-11 2019-01-15 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
WO2016138500A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Methods and compositions for barcoding nucleic acids for sequencing
ES2836802T3 (es) 2015-02-27 2021-06-28 Becton Dickinson Co Códigos de barras moleculares espacialmente direccionables
HK1250245A1 (zh) 2015-04-10 2018-12-07 Spatial Transcriptomics Ab 生物样本的空间区别、多重核酸分析
WO2017015097A1 (en) 2015-07-17 2017-01-26 Nanostring Technologies, Inc. Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue
EP4368728A3 (en) 2015-07-17 2024-08-07 Bruker Spatial Biology, Inc. Simultaneous quantification of gene expression in a user-defined region of a cross-sectioned tissue
ES2888626T3 (es) 2015-07-27 2022-01-05 Illumina Inc Cartografiado espacial de información de secuencia de ácidos nucleicos
WO2017044614A1 (en) 2015-09-11 2017-03-16 The Regents Of The University Of California Isoelectric focusing arrays and methods of use thereof
US10049770B2 (en) 2015-12-30 2018-08-14 Case Western Reserve University Prediction of recurrence of non-small cell lung cancer
US12060412B2 (en) 2016-03-21 2024-08-13 The Broad Institute, Inc. Methods for determining spatial and temporal gene expression dynamics in single cells
CN118853848A (zh) 2016-08-31 2024-10-29 哈佛学院董事及会员团体 将生物分子的检测组合到使用荧光原位测序的单个试验的方法
GB2570412A (en) 2016-08-31 2019-07-24 Harvard College Methods of generating libraries of nucleic acid sequences for detection via fluorescent in situ sequencing
GB201619458D0 (en) 2016-11-17 2017-01-04 Spatial Transcriptomics Ab Method for spatial tagging and analysing nucleic acids in a biological specimen
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4603594A3 (en) 2018-08-28 2025-11-12 10X Genomics, Inc. Increasing spatial array resolution
EP3864173B1 (en) 2018-10-10 2025-08-27 10x Genomics, Inc. Surface capture of target nucleic acid molecules
WO2020123318A1 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Resolving spatial arrays using deconvolution
US20210189475A1 (en) 2018-12-10 2021-06-24 10X Genomics, Inc. Imaging system hardware
WO2020219901A1 (en) 2019-04-26 2020-10-29 10X Genomics, Inc. Imaging support devices
US20210140982A1 (en) 2019-10-18 2021-05-13 10X Genomics, Inc. Identification of spatial biomarkers of brain disorders and methods of using the same
EP4055185A1 (en) 2019-11-08 2022-09-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
EP4589016A3 (en) 2019-11-13 2025-10-08 10x Genomics, Inc. Generating capture probes for spatial analysis
US20210199660A1 (en) 2019-11-22 2021-07-01 10X Genomics, Inc. Biomarkers of breast cancer
ES2982420T3 (es) 2019-12-23 2024-10-16 10X Genomics Inc Métodos para el análisis espacial mediante el uso de la ligazón con plantilla de ARN
US20210198741A1 (en) 2019-12-30 2021-07-01 10X Genomics, Inc. Identification of spatial biomarkers of heart disorders and methods of using the same
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210222253A1 (en) 2020-01-21 2021-07-22 10X Genomics, Inc. Identification of biomarkers of glioblastoma and methods of using the same
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries

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