SG11202111878RA - Method of detecting target nucleic acid molecules - Google Patents

Method of detecting target nucleic acid molecules

Info

Publication number
SG11202111878RA
SG11202111878RA SG11202111878RA SG11202111878RA SG11202111878RA SG 11202111878R A SG11202111878R A SG 11202111878RA SG 11202111878R A SG11202111878R A SG 11202111878RA SG 11202111878R A SG11202111878R A SG 11202111878RA SG 11202111878R A SG11202111878R A SG 11202111878RA
Authority
SG
Singapore
Prior art keywords
nucleic acid
target nucleic
acid molecules
detecting target
detecting
Prior art date
Application number
SG11202111878RA
Inventor
Malte Kühnemund
Toon Verheyen
Original Assignee
10X Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB1907779.1A external-priority patent/GB201907779D0/en
Priority claimed from GBGB1907752.8A external-priority patent/GB201907752D0/en
Priority claimed from GBGB1907764.3A external-priority patent/GB201907764D0/en
Priority claimed from GBGB1907772.6A external-priority patent/GB201907772D0/en
Application filed by 10X Genomics Inc filed Critical 10X Genomics Inc
Publication of SG11202111878RA publication Critical patent/SG11202111878RA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/137Reactions characterised by the reaction format or use of a specific feature the purpose or use of a displacement step
    • C12Q2537/1373Displacement by a nucleic acid

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG11202111878RA 2019-05-31 2020-05-29 Method of detecting target nucleic acid molecules SG11202111878RA (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GBGB1907779.1A GB201907779D0 (en) 2019-05-31 2019-05-31 Method of detecting target nucleic acid molecules
GBGB1907752.8A GB201907752D0 (en) 2019-05-31 2019-05-31 Method of decoding nucleotide barcode sequences
GBGB1907764.3A GB201907764D0 (en) 2019-05-31 2019-05-31 Method of decoding nucleotide barcode sequences
GBGB1907772.6A GB201907772D0 (en) 2019-05-31 2019-05-31 Method of detecting target nucleic acid molecules
PCT/EP2020/065090 WO2020240025A1 (en) 2019-05-31 2020-05-29 Method of detecting target nucleic acid molecules

Publications (1)

Publication Number Publication Date
SG11202111878RA true SG11202111878RA (en) 2021-11-29

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202111878RA SG11202111878RA (en) 2019-05-31 2020-05-29 Method of detecting target nucleic acid molecules

Country Status (7)

Country Link
US (3) US11555219B2 (en)
EP (1) EP3976817A1 (en)
CN (1) CN113906147B (en)
AU (1) AU2020282024A1 (en)
CA (1) CA3139791A1 (en)
SG (1) SG11202111878RA (en)
WO (1) WO2020240025A1 (en)

Families Citing this family (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017222453A1 (en) 2016-06-21 2017-12-28 Hauling Thomas Nucleic acid sequencing
CA3078158A1 (en) 2017-10-06 2019-04-11 Cartana Ab Rna templated ligation
US20220049294A1 (en) 2018-12-10 2022-02-17 10X Genomics, Inc. Imaging system hardware
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
CA3139791A1 (en) 2019-05-31 2020-12-03 10X Genomics, Inc. Method of detecting target nucleic acid molecules
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
ES2946357T3 (en) 2019-12-23 2023-07-17 10X Genomics Inc Methods for spatial analysis using RNA template ligation
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
CN115916999A (en) 2020-04-22 2023-04-04 10X基因组学有限公司 Methods for spatial analysis using targeted RNA depletion
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
EP4414459A3 (en) 2020-05-22 2024-09-18 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4162074B1 (en) 2020-06-08 2024-04-24 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4172362B1 (en) 2020-06-25 2024-09-18 10X Genomics, Inc. Spatial analysis of dna methylation
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US20220084629A1 (en) 2020-09-16 2022-03-17 10X Genomics, Inc. Systems and methods for barcode design and decoding
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4012046A1 (en) * 2020-12-11 2022-06-15 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
EP4121555A1 (en) 2020-12-21 2023-01-25 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
CN116848263A (en) 2020-12-23 2023-10-03 10X基因组学有限公司 Methods and compositions for analyte detection
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
WO2022164809A1 (en) 2021-01-26 2022-08-04 10X Genomics, Inc. Nucleic acid analog probes for in situ analysis
EP4301873A1 (en) 2021-03-03 2024-01-10 10X Genomics, Inc. Analyte detection in situ using nucleic acid origami
WO2022197801A1 (en) * 2021-03-17 2022-09-22 Yale University Systems and methods for determining barcodes and screening in situ
US20240309445A1 (en) * 2021-03-23 2024-09-19 Ultima Genomics, Inc. Methods for sequencing nucleic acid molecules with sequential barcodes
US20240254554A1 (en) * 2021-05-21 2024-08-01 The Board Of Trustees Of The Leland Stanford Junior University Sequencing Chemistries and Encodings for Next-Generation in Situ Sequencing
EP4347877A1 (en) * 2021-06-01 2024-04-10 10X Genomics, Inc. Methods and compositions for analyte detection and probe resolution
US20230084407A1 (en) 2021-06-02 2023-03-16 10X Genomics, Inc. Sample analysis using asymmetric circularizable probes
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
EP4370896A1 (en) 2021-07-13 2024-05-22 10X Genomics, Inc. Methods for preparing polymerized matrix with controllable thickness
WO2023015192A1 (en) 2021-08-03 2023-02-09 10X Genomics, Inc. Nucleic acid concatemers and methods for stabilizing and/or compacting the same
CN117858958A (en) 2021-08-16 2024-04-09 10X基因组学有限公司 Probes comprising segmented barcode regions and methods of use
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
WO2023108139A2 (en) 2021-12-10 2023-06-15 10X Genomics, Inc. Multi-resolution in situ decoding
WO2023129898A2 (en) 2021-12-27 2023-07-06 10X Genomics, Inc. Methods and compositions for rolling circle amplification
WO2023141588A1 (en) 2022-01-21 2023-07-27 10X Genomics, Inc. Multiple readout signals for analyzing a sample
WO2023172915A1 (en) 2022-03-08 2023-09-14 10X Genomics, Inc. In situ code design methods for minimizing optical crowding
WO2023192302A1 (en) 2022-03-29 2023-10-05 10X Genomics, Inc. Spectral unmixing combined with decoding for super-multiplexed in situ analysis
AU2023243205A1 (en) 2022-04-01 2024-09-19 10X Genomics, Inc. Compositions and methods for targeted masking of autofluorescence
US20230323427A1 (en) 2022-04-06 2023-10-12 10X Genomics, Inc. Methods and compositions for multiplex cell analysis
WO2023215612A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Analysis of antigen and antigen receptor interactions
WO2023215603A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Methods and compositions for in situ analysis of v(d)j sequences
US20240035071A1 (en) 2022-06-17 2024-02-01 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
WO2024036304A1 (en) 2022-08-12 2024-02-15 10X Genomics, Inc. Puma1 polymerases and uses thereof
WO2024040060A1 (en) 2022-08-16 2024-02-22 10X Genomics, Inc. Ap50 polymerases and uses thereof
WO2024081869A1 (en) 2022-10-14 2024-04-18 10X Genomics, Inc. Methods for analysis of biological samples
WO2024107887A1 (en) 2022-11-16 2024-05-23 10X Genomics, Inc. Methods and compositions for assessing performance of in situ assays
US20240218437A1 (en) 2022-12-16 2024-07-04 10X Genomics, Inc. Methods and compositions for assessing performance
US20240218424A1 (en) 2022-12-21 2024-07-04 10X Genomics, Inc. Methods for tethering ribonucleic acids in biological samples
US20240263219A1 (en) 2023-01-06 2024-08-08 10X Genomics, Inc. Methods and compositions for in situ analysis of variant sequences
US20240263220A1 (en) 2023-02-03 2024-08-08 10X Genomics, Inc. In situ analysis of variant sequences in biological samples
WO2024179837A1 (en) * 2023-03-01 2024-09-06 Resolve Biosciences Gmbh Complex formation for parallel detection of multiple analytes in multiple applications

Family Cites Families (149)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4883867A (en) 1985-11-01 1989-11-28 Becton, Dickinson And Company Detection of reticulocytes, RNA or DNA
US5525464A (en) 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US5200314A (en) 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
US5942391A (en) 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5648245A (en) * 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
US6534266B1 (en) 1999-04-22 2003-03-18 Albert Einstein College Of Medicine Of Yeshiva University Assay of transcription sites by multi-fluor fish
ATE380883T1 (en) * 2000-10-24 2007-12-15 Univ Leland Stanford Junior DIRECT MULTIPLEX CHARACTERIZATION OF GENOMIC DNA
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
WO2004076683A2 (en) 2003-02-26 2004-09-10 Callida Genomics, Inc. Random array dna analysis by hybridization
WO2005047543A2 (en) 2003-06-10 2005-05-26 Applera Corporation Ligation assay
EP1846869B1 (en) 2005-01-27 2011-10-05 Cambridge Research & Instrumentation, Inc. Classifying image features
JP2008528040A (en) 2005-02-01 2008-07-31 アジェンコート バイオサイエンス コーポレイション Reagents, methods and libraries for bead-based sequencing
US7727721B2 (en) 2005-03-08 2010-06-01 California Institute Of Technology Hybridization chain reaction amplification for in situ imaging
WO2006108422A2 (en) 2005-04-12 2006-10-19 In Situ Rcp A/S Under Founding Methods for production of oligonucleotides
WO2007120208A2 (en) 2005-11-14 2007-10-25 President And Fellows Of Harvard College Nanogrid rolling circle dna sequencing
WO2007076132A2 (en) 2005-12-23 2007-07-05 Nanostring Technologies, Inc. Compositions comprising oriented, immobilized macromolecules and methods for their preparation
CA2640385C (en) 2005-12-23 2014-07-15 Nanostring Technologies, Inc. Nanoreporters and methods of manufacturing and use thereof
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
GB0605584D0 (en) 2006-03-20 2006-04-26 Olink Ab Method for analyte detection using proximity probes
ES2620398T3 (en) 2006-05-22 2017-06-28 Nanostring Technologies, Inc. Systems and methods to analyze nanoindicators
US9201063B2 (en) 2006-11-16 2015-12-01 General Electric Company Sequential analysis of biological samples
US7655898B2 (en) 2006-11-30 2010-02-02 Cambridge Research & Instrumentation, Inc. Optical filter assembly with selectable bandwidth and rejection
WO2008124847A2 (en) 2007-04-10 2008-10-16 Nanostring Technologies, Inc. Methods and computer systems for identifying target-specific sequences for use in nanoreporters
WO2008151127A1 (en) 2007-06-04 2008-12-11 President And Fellows Of Harvard College Compounds and methods for chemical ligation
US7534991B2 (en) 2007-07-10 2009-05-19 Cambridge Research & Instrumentation, Inc. Athermalized birefringent filter apparatus and method
US8330087B2 (en) 2007-10-16 2012-12-11 Cambridge Research & Instrumentation, Inc. Spectral imaging system with dynamic optical correction
US8199999B2 (en) 2008-06-17 2012-06-12 Cambridge Research & Instrumentation, Inc. Image classifier training
EP2331704B1 (en) 2008-08-14 2016-11-30 Nanostring Technologies, Inc Stable nanoreporters
ES2627063T3 (en) 2009-10-13 2017-07-26 Nanostring Technologies, Inc Protein detection through nanoinformers
EP2516681B1 (en) 2010-02-11 2017-10-18 Nanostring Technologies, Inc Compositions and methods for the detection of preferably small rnas by bridge hybridisation and ligation
US20110257031A1 (en) * 2010-02-12 2011-10-20 Life Technologies Corporation Nucleic acid, biomolecule and polymer identifier codes
US10267808B2 (en) 2010-03-08 2019-04-23 California Institute Of Technology Molecular indicia of cellular constituents and resolving the same by super-resolution technologies in single cells
GB201004292D0 (en) 2010-03-15 2010-04-28 Olink Ab Assay for localised detection of analytes
AU2011237729B2 (en) 2010-04-05 2014-04-03 Prognosys Biosciences, Inc. Spatially encoded biological assays
US8462981B2 (en) 2010-04-07 2013-06-11 Cambridge Research & Instrumentation, Inc. Spectral unmixing for visualization of samples
US9541504B2 (en) 2010-08-05 2017-01-10 Cambridge Research & Instrumentation, Inc. Enhancing visual assessment of samples
EP2627781B1 (en) 2010-10-15 2017-02-22 Olink Bioscience AB Dynamic range methods
EP3034625B1 (en) 2010-10-21 2017-10-04 Advanced Cell Diagnostics, Inc. An ultra sensitive method for in situ detection of nucleic acids
US20130323729A1 (en) 2010-10-29 2013-12-05 Olink Ab Proximity Ligation Technology for Western Blot Applications
US8551708B2 (en) 2011-02-15 2013-10-08 Leica Biosystems Newcastle Ltd. Methods for localized in situ detection of mRNA
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US8946389B2 (en) 2011-04-25 2015-02-03 University Of Washington Compositions and methods for multiplex biomarker profiling
GB201107863D0 (en) 2011-05-11 2011-06-22 Olink Ab Method and product
GB201108678D0 (en) 2011-05-24 2011-07-06 Olink Ab Multiplexed proximity ligation assay
JP2014531908A (en) 2011-10-14 2014-12-04 プレジデント アンド フェローズ オブ ハーバード カレッジ Sequencing by structural assembly
EP4108782B1 (en) 2011-12-22 2023-06-07 President and Fellows of Harvard College Compositions and methods for analyte detection
US11021737B2 (en) 2011-12-22 2021-06-01 President And Fellows Of Harvard College Compositions and methods for analyte detection
WO2013177046A1 (en) * 2012-05-21 2013-11-28 Solulink, Inc. Methods and/or use of oligonucleotide conjugates for suppressing background due to cross-hybridization
WO2013177245A2 (en) 2012-05-22 2013-11-28 Nanostring Technologies, Inc. Nano46 genes and methods to predict breast cancer outcome
US9914967B2 (en) 2012-06-05 2018-03-13 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using DNA origami probes
EP4163617A1 (en) 2012-08-09 2023-04-12 The Board of Trustees of the Leland Stanford Junior University Methods and compositions for preparing biological specimens for microscopic analysis
CN104704348A (en) 2012-08-21 2015-06-10 剑桥研究和仪器设备股份有限公司 Visualization and measurement of cell compartments
US9783841B2 (en) 2012-10-04 2017-10-10 The Board Of Trustees Of The Leland Stanford Junior University Detection of target nucleic acids in a cellular sample
US10174366B2 (en) 2012-11-14 2019-01-08 Olink Bioscience Ab Localised RCA-based amplification method
US10964001B2 (en) 2013-01-10 2021-03-30 Akoya Biosciences, Inc. Multispectral imaging systems and methods
WO2014110025A1 (en) 2013-01-10 2014-07-17 Caliper Life Sciences, Inc. Whole slide multispectral imaging systems and methods
US10138509B2 (en) 2013-03-12 2018-11-27 President And Fellows Of Harvard College Method for generating a three-dimensional nucleic acid containing matrix
US20140342354A1 (en) * 2013-03-12 2014-11-20 Counsyl, Inc. Systems and methods for prenatal genetic analysis
US9273349B2 (en) 2013-03-14 2016-03-01 Affymetrix, Inc. Detection of nucleic acids
US11441196B2 (en) 2013-03-15 2022-09-13 The Broad Institute, Inc. Ribosomal ribonucleic acid hybridization for organism identification
US10510435B2 (en) 2013-04-30 2019-12-17 California Institute Of Technology Error correction of multiplex imaging analysis by sequential hybridization
DK2992115T3 (en) 2013-04-30 2020-06-02 California Inst Of Techn Multiplexing of molecules by bar coding with sequential hybridization
EP4234716A3 (en) 2013-06-25 2023-12-06 Prognosys Biosciences, Inc. Methods for determining spatial patterns of biological targets in a sample
WO2015041755A1 (en) 2013-09-20 2015-03-26 California Institute Of Technology Methods for phenotyping of intact whole tissues
US10794802B2 (en) 2013-09-20 2020-10-06 California Institute Of Technology Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high resolution intact circuit mapping and phenotyping
GB201401885D0 (en) 2014-02-04 2014-03-19 Olink Ab Proximity assay with detection based on hybridisation chain reaction (HCR)
US10309879B2 (en) 2014-02-21 2019-06-04 Massachusetts Institute Of Technology Expansion microscopy
WO2015148606A2 (en) 2014-03-25 2015-10-01 President And Fellows Of Harvard College Barcoded protein array for multiplex single-molecule interaction profiling
US10746981B2 (en) 2014-05-30 2020-08-18 The Board Of Trustees Of The Leland Stanford Junior University Methods and devices for imaging large intact tissue samples
US9909167B2 (en) 2014-06-23 2018-03-06 The Board Of Trustees Of The Leland Stanford Junior University On-slide staining by primer extension
US10126242B2 (en) 2014-07-09 2018-11-13 Caliper Life Sciences, Inc. Pure spectrum extraction from biological samples in fluorescence multispectral imaging
US10179932B2 (en) 2014-07-11 2019-01-15 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
WO2016011429A1 (en) 2014-07-17 2016-01-21 California Institute Of Technology Multiplex analysis of molecules in single cells by image correlation
WO2016018963A1 (en) 2014-07-30 2016-02-04 President And Fellows Of Harvard College Probe library construction
KR20170036801A (en) 2014-08-19 2017-04-03 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 Rna-guided systems for probing and mapping of nucleic acids
US11091810B2 (en) 2015-01-27 2021-08-17 BioSpyder Technologies, Inc. Focal gene expression profiling of stained FFPE tissues with spatial correlation to morphology
US20160108458A1 (en) 2014-10-06 2016-04-21 The Board Of Trustees Of The Leland Stanford Junior University Multiplexed detection and quantification of nucleic acids in single-cells
AU2015349870B2 (en) 2014-11-21 2019-09-26 Bruker Spatial Biology, Inc. Enzyme- and amplification-free sequencing
EP3223947B1 (en) 2014-11-24 2019-10-30 Nanostring Technologies, Inc. Methods and apparatuses for gene purification and imaging
ES2906221T3 (en) * 2015-02-27 2022-04-13 Becton Dickinson Co Methods for barcoding nucleic acids for sequencing
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
CA2982146A1 (en) 2015-04-10 2016-10-13 Spatial Transcriptomics Ab Spatially distinguished, multiplex nucleic acid analysis of biological specimens
US10526649B2 (en) 2015-04-14 2020-01-07 Massachusetts Institute Of Technology Augmenting in situ nucleic acid sequencing of expanded biological samples with in vitro sequence information
US11408890B2 (en) 2015-04-14 2022-08-09 Massachusetts Institute Of Technology Iterative expansion microscopy
US10059990B2 (en) 2015-04-14 2018-08-28 Massachusetts Institute Of Technology In situ nucleic acid sequencing of expanded biological samples
EP3325650B1 (en) 2015-07-17 2024-01-03 Nanostring Technologies, Inc. Simultaneous quantification of gene expression in a user-defined region of a cross-sectioned tissue
EP4368992A3 (en) 2015-07-17 2024-08-07 Bruker Spatial Biology, Inc. Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue
DK3325669T3 (en) 2015-07-24 2023-10-09 Univ Johns Hopkins Compositions and methods for RNA analysis
EP3332029B1 (en) 2015-08-07 2021-10-06 Massachusetts Institute of Technology Nanoscale imaging of proteins and nucleic acids via expansion microscopy
WO2017027368A1 (en) 2015-08-07 2017-02-16 Massachusetts Institute Of Technology Protein retention expansion microscopy
US11214661B2 (en) 2015-09-17 2022-01-04 Massachusetts Institute Of Technology Three-dimensional nanofabrication by patterning of hydrogels
CN108368543B (en) 2015-10-12 2023-06-13 领先细胞医疗诊断有限公司 In situ detection of nucleotide variants in high noise samples and related compositions and methods
US10802262B2 (en) 2015-10-29 2020-10-13 The Board Of Trustees Of The Leland Stanford Junior University Methods and systems for imaging a biological sample
US20190155835A1 (en) 2015-11-03 2019-05-23 President And Fellows Of Harvard College Systems and Methods for Processing Spatially Related Sequence Data Received from a Sequencing Device
MX2018005611A (en) 2015-11-03 2018-11-09 Harvard College Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix.
US11254974B2 (en) 2016-02-10 2022-02-22 The Board Of Trustees Of The Leland Stanford Junior University RNA fixation and detection in clarity-based hydrogel tissue
US20210207131A1 (en) 2016-02-18 2021-07-08 President And Fellows Of Harvard College Multiplex Alteration of Cells Using a Pooled Nucleic Acid Library and Analysis Thereof
US11360025B2 (en) 2016-02-22 2022-06-14 Miltenyi Biotec B.V. & Co. KG Automated analysis tool for biological specimens
WO2017147483A1 (en) 2016-02-26 2017-08-31 The Board Of Trustees Of The Leland Stanford Junior University Multiplexed single molecule rna visualization with a two-probe proximity ligation system
US20170253918A1 (en) 2016-03-01 2017-09-07 Expansion Technologies Combining protein barcoding with expansion microscopy for in-situ, spatially-resolved proteomics
WO2017161251A1 (en) * 2016-03-17 2017-09-21 President And Fellows Of Harvard College Methods for detecting and identifying genomic nucleic acids
GB2565461B (en) 2016-03-31 2022-04-13 Harvard College Methods and compositions for the single tube preparation of sequencing libraries using Cas9
CN116200465A (en) 2016-04-25 2023-06-02 哈佛学院董事及会员团体 Hybrid chain reaction method for in situ molecular detection
US20180052081A1 (en) 2016-05-11 2018-02-22 Expansion Technologies Combining modified antibodies with expansion microscopy for in-situ, spatially-resolved proteomics
KR102490693B1 (en) 2016-05-16 2023-01-19 나노스트링 테크놀로지스, 인크. Method for detecting target nucleic acid in a sample
EP3252452A1 (en) 2016-05-25 2017-12-06 The Board of Trustees of the Leland Stanford Junior University Method for imaging and analysis of a biological specimen
WO2017222453A1 (en) 2016-06-21 2017-12-28 Hauling Thomas Nucleic acid sequencing
IL302802A (en) 2016-07-05 2023-07-01 California Inst Of Techn Fractional initiator hybridization chain reaction
CN109789228B (en) 2016-07-27 2022-10-21 斯坦福大学托管董事会 Highly multiplexed fluorescence imaging
US20210017587A1 (en) 2016-08-01 2021-01-21 California Institute Of Technology Sequential probing of molecular targets based on pseudo-color barcodes with embedded error correction mechanism
IL264831B (en) 2016-08-30 2022-09-01 California Inst Of Techn Immunohistochemistry via hybridization chain reaction
CN109983125B (en) 2016-08-31 2024-06-04 哈佛学院董事及会员团体 Method for generating a library of nucleic acid sequences for detection by fluorescence in situ sequencing
CN109923216B (en) 2016-08-31 2024-08-02 哈佛学院董事及会员团体 Method of combining detection of biomolecules into a single assay using fluorescence in situ sequencing
EP4339956A3 (en) 2016-11-08 2024-06-19 President and Fellows of Harvard College Multiplexed imaging using merfish, expansion microscopy, and related technologies
WO2018089445A1 (en) 2016-11-08 2018-05-17 President And Fellows Of Harvard College Matrix imprinting and clearing
KR102476709B1 (en) * 2016-11-21 2022-12-09 나노스트링 테크놀로지스, 인크. Chemical compositions and methods of using same
WO2018132392A2 (en) 2017-01-10 2018-07-19 President And Fellows Of Harvard College Multiplexed signal amplification
US20200224243A1 (en) 2017-03-22 2020-07-16 The Board Of Trustees Of The Leland Stanford Junior University Proximity Ligation in Situ Hybridization (PLISH)
WO2019023214A1 (en) 2017-07-25 2019-01-31 Massachusetts Institute Of Technology In situ atac sequencing
CA3078158A1 (en) 2017-10-06 2019-04-11 Cartana Ab Rna templated ligation
WO2019075091A1 (en) 2017-10-11 2019-04-18 Expansion Technologies Multiplexed in situ hybridization of tissue sections for spatially resolved transcriptomics with expansion microscopy
US11479811B2 (en) 2017-11-21 2022-10-25 Expansion Technologies Expansion microscopy compatible and multiplexed in situ hybridization of formalin fixed paraffin embedded tissue sections for spatially resolved transcriptomics
AU2018379082A1 (en) 2017-12-08 2020-06-11 California Institute Of Technology Multiplex labeling of molecules by sequential hybridization barcoding with rapid switching and rehybridzation of probes
KR20200121329A (en) 2018-02-12 2020-10-23 나노스트링 테크놀로지스, 인크. Biomolecular probes and methods for detecting gene and protein expression
JP7502787B2 (en) 2018-04-09 2024-06-19 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー Methods for in situ gene sequencing
CN112384787A (en) 2018-05-03 2021-02-19 阿科亚生物科学股份有限公司 Multispectral sample imaging
SG11202101934SA (en) 2018-07-30 2021-03-30 Readcoor Llc Methods and systems for sample processing or analysis
CN112930404A (en) 2018-09-05 2021-06-08 瑞德库尔有限责任公司 Methods and systems for therapeutic agent analysis
WO2020076979A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Surface capture of targets
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
CN113195735A (en) 2018-10-19 2021-07-30 阿科亚生物科学股份有限公司 Detection of coexisting receptor-encoding nucleic acid segments
GB201818742D0 (en) 2018-11-16 2019-01-02 Cartana Ab Method for detection of RNA
JP7332694B2 (en) 2018-12-04 2023-08-23 エフ. ホフマン-ラ ロシュ アーゲー Spatial directional quantum barcoding of cellular targets
US20220049294A1 (en) 2018-12-10 2022-02-17 10X Genomics, Inc. Imaging system hardware
US20220064697A1 (en) 2018-12-13 2022-03-03 President And Fellows Of Harvard College Amplification methods and systems for merfish and other applications
WO2020142490A1 (en) 2018-12-31 2020-07-09 Expansion Technologies Expansion microscopy compatible anchorable h&e staining for histopathology
CA3139791A1 (en) 2019-05-31 2020-12-03 10X Genomics, Inc. Method of detecting target nucleic acid molecules
US11994452B2 (en) 2019-06-14 2024-05-28 Akoya Biosciences, Inc. Multiplexed tissue imaging
EP3754028A1 (en) 2019-06-18 2020-12-23 Apollo Life Sciences GmbH Method of signal encoding of analytes in a sample
GB201919029D0 (en) 2019-12-20 2020-02-05 Cartana Ab Method of detecting an analyte
GB201919032D0 (en) 2019-12-20 2020-02-05 Cartana Ab Method of detecting an analyte
EP4097251A1 (en) 2020-01-29 2022-12-07 10X Genomics, Inc. Compositions and methods for analyte detection
CN115461143A (en) 2020-02-21 2022-12-09 10X基因组学有限公司 Compositions, methods, and systems for sample processing
US20220084629A1 (en) 2020-09-16 2022-03-17 10X Genomics, Inc. Systems and methods for barcode design and decoding
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
EP4012046A1 (en) 2020-12-11 2022-06-15 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
CN116848263A (en) 2020-12-23 2023-10-03 10X基因组学有限公司 Methods and compositions for analyte detection
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes

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