PT3882357T - Métodos e composições para análise de ácidos nucleicos - Google Patents

Métodos e composições para análise de ácidos nucleicos

Info

Publication number
PT3882357T
PT3882357T PT211574215T PT21157421T PT3882357T PT 3882357 T PT3882357 T PT 3882357T PT 211574215 T PT211574215 T PT 211574215T PT 21157421 T PT21157421 T PT 21157421T PT 3882357 T PT3882357 T PT 3882357T
Authority
PT
Portugal
Prior art keywords
compositions
methods
nucleic acid
acid analysis
analysis
Prior art date
Application number
PT211574215T
Other languages
English (en)
Portuguese (pt)
Original Assignee
10X Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=57714651&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=PT3882357(T) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by 10X Genomics Inc filed Critical 10X Genomics Inc
Publication of PT3882357T publication Critical patent/PT3882357T/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2543/00—Reactions characterised by the reaction site, e.g. cell or chromosome
    • C12Q2543/10—Reactions characterised by the reaction site, e.g. cell or chromosome the purpose being "in situ" analysis
    • C12Q2543/101—Reactions characterised by the reaction site, e.g. cell or chromosome the purpose being "in situ" analysis in situ amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
PT211574215T 2015-12-04 2016-12-02 Métodos e composições para análise de ácidos nucleicos PT3882357T (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201562263532P 2015-12-04 2015-12-04

Publications (1)

Publication Number Publication Date
PT3882357T true PT3882357T (pt) 2022-09-05

Family

ID=57714651

Family Applications (1)

Application Number Title Priority Date Filing Date
PT211574215T PT3882357T (pt) 2015-12-04 2016-12-02 Métodos e composições para análise de ácidos nucleicos

Country Status (12)

Country Link
US (7) US10774370B2 (enExample)
EP (5) EP4782550A3 (enExample)
JP (2) JP6954899B2 (enExample)
KR (2) KR102723865B1 (enExample)
CN (2) CN108431232B (enExample)
DE (1) DE202016009134U1 (enExample)
DK (1) DK3882357T3 (enExample)
ES (1) ES2926495T3 (enExample)
IL (1) IL259736A (enExample)
PT (1) PT3882357T (enExample)
SG (2) SG11201804086VA (enExample)
WO (1) WO2017096158A1 (enExample)

Families Citing this family (149)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN114891871A (zh) 2012-08-14 2022-08-12 10X基因组学有限公司 微胶囊组合物及方法
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
KR20230003659A (ko) 2013-02-08 2023-01-06 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
CN111500680B (zh) 2013-06-25 2024-04-16 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
US9694361B2 (en) 2014-04-10 2017-07-04 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
CN114214314A (zh) 2014-06-24 2022-03-22 生物辐射实验室股份有限公司 数字式pcr条码化
EP4574974A3 (en) 2014-06-26 2025-10-08 10x Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
KR20170026383A (ko) 2014-06-26 2017-03-08 10엑스 제노믹스, 인크. 핵산 서열의 분석
AU2016207023B2 (en) 2015-01-12 2019-12-05 10X Genomics, Inc. Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
EP4286516A3 (en) 2015-02-24 2024-03-06 10X Genomics, Inc. Partition processing methods and systems
WO2016154540A1 (en) 2015-03-26 2016-09-29 Dovetail Genomics Llc Physical linkage preservation in dna storage
EP4321627A3 (en) 2015-04-10 2024-04-17 10x Genomics Sweden AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
US12018314B2 (en) 2015-07-02 2024-06-25 Arima Genomics, Inc. Accurate molecular deconvolution of mixture samples
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
KR102723865B1 (ko) 2015-12-04 2024-10-29 10엑스 제노믹스, 인크. 핵산 분석을 위한 방법 및 조성물
JP6735348B2 (ja) 2016-02-11 2020-08-05 １０エックス ジェノミクス， インコーポレイテッド 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体
WO2017197300A1 (en) 2016-05-13 2017-11-16 Dovetail Genomics Llc Recovering long-range linkage information from preserved samples
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
EP4310183B1 (en) 2017-01-30 2025-07-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
WO2018145041A1 (en) 2017-02-06 2018-08-09 10X Genomics, Inc. Systems and methods for nucleic acid preparation
EP3625353B1 (en) 2017-05-18 2022-11-30 10X Genomics, Inc. Methods and systems for sorting droplets and beads
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN116064732A (zh) 2017-05-26 2023-05-05 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
US10549279B2 (en) 2017-08-22 2020-02-04 10X Genomics, Inc. Devices having a plurality of droplet formation regions
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
EP3691703A1 (en) 2017-10-04 2020-08-12 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
WO2019084328A1 (en) * 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS FOR PREPARING NUCLEIC ACID MOLECULES
CN111479631B (zh) 2017-10-27 2022-02-22 10X基因组学有限公司 用于样品制备和分析的方法和系统
EP3625361A1 (en) 2017-11-15 2020-03-25 10X Genomics, Inc. Functionalized gel beads
WO2019099908A1 (en) 2017-11-17 2019-05-23 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
EP3714052A1 (en) * 2017-11-21 2020-09-30 Arima Genomics, Inc. Preserving spatial-proximal contiguity and molecular contiguity in nucleic acid templates
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
CN118818037A (zh) 2017-12-12 2024-10-22 10X基因组学有限公司 用于单细胞处理的系统和方法
WO2019126789A1 (en) 2017-12-22 2019-06-27 10X Genomics, Inc. Systems and methods for processing nucleic acid molecules from one or more cells
WO2019148042A1 (en) * 2018-01-26 2019-08-01 10X Genomics, Inc. Compositions and methods for sample processing
EP3752832A1 (en) 2018-02-12 2020-12-23 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
EP3775271B1 (en) 2018-04-06 2025-03-12 10X Genomics, Inc. Systems and methods for quality control in single cell processing
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US12163179B2 (en) 2018-08-03 2024-12-10 10X Gemomics, Inc. Methods and systems to minimize barcode exchange
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
CN113767175B (zh) 2018-08-28 2025-07-25 10X基因组学股份有限公司 增加空间阵列分辨率
EP3894585A2 (en) 2018-12-10 2021-10-20 10X Genomics, Inc. Generating capture probes for spatial analysis
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
WO2020167862A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transfer of reagents between droplets
US12275993B2 (en) 2019-02-12 2025-04-15 10X Genomics, Inc. Analysis of nucleic acid sequences
US12305239B2 (en) 2019-02-12 2025-05-20 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2020168013A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Methods for processing nucleic acid molecules
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
SG11202111242PA (en) 2019-03-11 2021-11-29 10X Genomics Inc Systems and methods for processing optically tagged beads
EP3947722A1 (en) 2019-03-27 2022-02-09 10X Genomics, Inc. Systems and methods for processing rna from cells
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
EP4752521A3 (en) 2019-12-23 2026-08-12 10X Genomics, Inc. Reversible fixing reagents and methods of use thereof
EP4081656A1 (en) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
CN121022991A (zh) 2019-12-23 2025-11-28 10X基因组学有限公司 使用rna模板化连接进行空间分析的方法
EP4628596A3 (en) * 2020-01-10 2025-12-31 10X Genomics, Inc. METHODS FOR DETERMINING THE LOCATION OF A TARGET NUCLEIC ACID IN A BIOLOGICAL SAMPLE
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US12129516B2 (en) 2020-02-07 2024-10-29 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US12399123B1 (en) 2020-02-14 2025-08-26 10X Genomics, Inc. Spatial targeting of analytes
CN116157533A (zh) 2020-02-21 2023-05-23 10X基因组学有限公司 使用杂交方法捕获遗传靶标
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) * 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
EP4139485B1 (en) 2020-04-22 2023-09-06 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
US12416603B2 (en) 2020-05-19 2025-09-16 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
EP4153775B1 (en) 2020-05-22 2024-07-24 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
EP4025692A2 (en) 2020-06-02 2022-07-13 10X Genomics, Inc. Nucleic acid library methods
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
EP4600376A3 (en) 2020-06-02 2025-10-22 10X Genomics, Inc. Spatial transcriptomics for antigen-receptors
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4421186B1 (en) 2020-06-08 2025-08-13 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4446430A3 (en) 2020-06-10 2024-12-18 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
US12435363B1 (en) 2020-06-10 2025-10-07 10X Genomics, Inc. Materials and methods for spatial transcriptomics
EP4164796A4 (en) 2020-06-10 2024-03-06 10x Genomics, Inc. Fluid delivery methods
EP4450639B1 (en) 2020-06-25 2025-10-15 10X Genomics, Inc. Spatial analysis of dna methylation
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US12553898B1 (en) 2020-08-10 2026-02-17 10X Genomics, Inc. Fluorescent hybridization of antibody-oligonucleotide for multiplexing and signal amplification
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
CN116194590A (zh) 2020-09-18 2023-05-30 10X基因组学有限公司 样品处理设备和图像配准方法
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US12655450B1 (en) 2020-10-27 2026-06-16 10X Genomics, Inc. Methods, systems, and kits for detection of nucleic acid molecules
US12480158B1 (en) 2020-11-05 2025-11-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US12398262B1 (en) 2021-01-22 2025-08-26 10X Genomics, Inc. Triblock copolymer-based cell stabilization and fixation system and methods of use thereof
ES2989523T3 (es) 2021-02-19 2024-11-26 10X Genomics Inc Método de uso de un dispositivo modular de soporte para ensayos
AU2022227563A1 (en) 2021-02-23 2023-08-24 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
EP4524261B1 (en) 2021-03-18 2026-07-22 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
EP4305196B1 (en) 2021-04-14 2025-04-02 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
ES3030033T3 (en) 2021-06-03 2025-06-26 10X Genomics Inc Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
US12553805B2 (en) 2021-08-02 2026-02-17 10X Genomics, Inc. Methods of preserving a biological sample
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
EP4419707A1 (en) 2021-11-10 2024-08-28 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023086847A1 (en) 2021-11-10 2023-05-19 Encodia, Inc. Methods for barcoding macromolecules in individual cells
EP4305195A2 (en) 2021-12-01 2024-01-17 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
EP4441711A1 (en) 2021-12-20 2024-10-09 10X Genomics, Inc. Self-test for pathology/histology slide imaging device
US12637706B2 (en) 2022-03-23 2026-05-26 10X Genomics, Inc. Methods and compositions related to microsphere surface gelation
WO2023204594A1 (ko) * 2022-04-19 2023-10-26 서울대학교산학협력단 태그멘테이션을 이용한 벡터 삽입위치 검출 및 클론 정량 방법
EP4536849B1 (en) 2022-06-06 2026-07-01 Genentech, Inc. COMBINATORIAL INDEXING FOR SINGLE-CELL NUCLEIC ACID SEQUENCING
EP4685239A1 (en) 2022-11-09 2026-01-28 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample

Family Cites Families (570)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2797149A (en) 1953-01-08 1957-06-25 Technicon International Ltd Methods of and apparatus for analyzing liquids containing crystalloid and non-crystalloid constituents
US3047367A (en) 1959-12-01 1962-07-31 Technicon Instr Automatic analysis with fluid segmentation
US3479141A (en) 1967-05-17 1969-11-18 Technicon Corp Method and apparatus for analysis
US4124638A (en) 1977-09-12 1978-11-07 Hansen John N Solubilizable polyacrylamide gels containing disulfide cross-linkages
JPS5949832B2 (ja) 1978-07-18 1984-12-05 ブラザー工業株式会社 電動ミシンにおける主軸定位置停止装置
US4253846A (en) 1979-11-21 1981-03-03 Technicon Instruments Corporation Method and apparatus for automated analysis of fluid samples
GB2097692B (en) 1981-01-10 1985-05-22 Shaw Stewart P D Combining chemical reagents
DE3230289A1 (de) 1982-08-14 1984-02-16 Bayer Ag, 5090 Leverkusen Herstellung von pharmazeutischen oder kosmetischen dispersionen
US4582802A (en) 1983-09-30 1986-04-15 The United States Of America As Represented By The Department Of Health And Human Services Stimulation of enzymatic ligation of DNA by high concentrations of nonspecific polymers
JPS60227826A (ja) 1984-04-27 1985-11-13 Sogo Yatsukou Kk ｐＨに応答するグラフトカプセル
US4916070A (en) 1986-04-14 1990-04-10 The General Hospital Corporation Fibrin-specific antibodies and method of screening for the antibodies
US5618711A (en) 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
US4872895A (en) 1986-12-11 1989-10-10 American Telephone And Telegraph Company, At&T Bell Laboratories Method for fabricating articles which include high silica glass bodies
US5525464A (en) 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US5202231A (en) 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
US5149625A (en) 1987-08-11 1992-09-22 President And Fellows Of Harvard College Multiplex analysis of DNA
US5137829A (en) 1987-10-05 1992-08-11 Washington University DNA transposon TN5SEQ1
US5185099A (en) 1988-04-20 1993-02-09 Institut National De Recherche Chimique Appliquee Visco-elastic, isotropic materials based on water, fluorinate sufactants and fluorinated oils, process for their preparation, and their use in various fields, such as optics, pharmacology and electrodynamics
US5237016A (en) 1989-01-05 1993-08-17 Siska Diagnostics, Inc. End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids
US5756334A (en) 1990-04-26 1998-05-26 New England Biolabs, Inc. Thermostable DNA polymerase from 9°N-7 and methods for producing the same
EP0540693B1 (en) 1990-07-24 1999-01-20 F. Hoffmann-La Roche Ag THE REDUCTION OF NON-SPECIFIC AMPLIFICATION DURING $i(IN VITRO) NUCLEIC ACID AMPLIFICATION USING MODIFIED NUCLEIC ACID BASES
US5489523A (en) 1990-12-03 1996-02-06 Stratagene Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I
US6582908B2 (en) 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
US5270183A (en) 1991-02-08 1993-12-14 Beckman Research Institute Of The City Of Hope Device and method for the automated cycling of solutions between two or more temperatures
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
WO1993001498A1 (en) 1991-07-04 1993-01-21 Immunodex K/S Water-soluble, polymer-based reagents and conjugates comprising moieties derived from divinyl sulfone
EP0604552B1 (en) 1991-09-18 1997-02-12 Affymax Technologies N.V. Method of synthesizing diverse collections of oligomers
US5413924A (en) 1992-02-13 1995-05-09 Kosak; Kenneth M. Preparation of wax beads containing a reagent for release by heating
WO1993019205A1 (en) 1992-03-19 1993-09-30 The Regents Of The University Of California Multiple tag labeling method for dna sequencing
US5587128A (en) 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
ATE140880T1 (de) 1992-05-01 1996-08-15 Univ Pennsylvania Fluessigkeitsbehandlung in mikrofabrizierten analytischen vorrichtungen
US5840865A (en) 1992-09-14 1998-11-24 Institute Of Molecular Biology And Biotechnology/Forth Eukaryotic transposable element
US5897783A (en) 1992-09-24 1999-04-27 Amersham International Plc Magnetic separation method
US5569364A (en) 1992-11-05 1996-10-29 Soane Biosciences, Inc. Separation media for electrophoresis
CA2155186A1 (en) 1993-02-01 1994-08-18 Kevin M. Ulmer Methods and apparatus for dna sequencing
AU6175594A (en) 1993-02-16 1994-09-14 Alliance Pharmaceutical Corporation Method of microemulsifying fluorinated oils
WO1994023699A1 (en) 1993-04-19 1994-10-27 Medisorb Technologies International L.P. Long-acting treatment by slow-release delivery of antisense oligodeoxyribonucleotides from biodegradable microparticles
DE69429038T2 (de) 1993-07-28 2002-03-21 Pe Corporation (Ny), Norwalk Vorrichtung und Verfahren zur Nukleinsäurevervielfältigung
US5874239A (en) 1993-07-30 1999-02-23 Affymax Technologies N.V. Biotinylation of proteins
US5512131A (en) 1993-10-04 1996-04-30 President And Fellows Of Harvard College Formation of microstamped patterns on surfaces and derivative articles
US20030044777A1 (en) 1993-10-28 2003-03-06 Kenneth L. Beattie Flowthrough devices for multiple discrete binding reactions
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5558071A (en) 1994-03-07 1996-09-24 Combustion Electromagnetics, Inc. Ignition system power converter and controller
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
CA2189958C (en) 1994-05-11 2005-04-19 Adrian Parton Methods of capturing species from liquids and assay procedures
US5705628A (en) 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5585069A (en) 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
EP0812434B1 (en) 1995-03-01 2013-09-18 President and Fellows of Harvard College Microcontact printing on surfaces and derivative articles
US5700642A (en) 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers
EP0832287B1 (en) 1995-06-07 2007-10-10 Solexa, Inc Oligonucleotide tags for sorting and identification
CZ392697A3 (cs) 1995-06-07 1998-06-17 Lynx Therapeutics, Inc. Oligonukleotidové značky pro stanovení druhu a identifikaci
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US6866760B2 (en) 1998-08-27 2005-03-15 E Ink Corporation Electrophoretic medium and process for the production thereof
US5872010A (en) 1995-07-21 1999-02-16 Northeastern University Microscale fluid handling system
US6057149A (en) 1995-09-15 2000-05-02 The University Of Michigan Microscale devices and reactions in microscale devices
US5851769A (en) 1995-09-27 1998-12-22 The Regents Of The University Of California Quantitative DNA fiber mapping
US5736330A (en) 1995-10-11 1998-04-07 Luminex Corporation Method and compositions for flow cytometric determination of DNA sequences
US5736332A (en) 1995-11-30 1998-04-07 Mandecki; Wlodek Method of determining the sequence of nucleic acids employing solid-phase particles carrying transponders
US6051377A (en) 1995-11-30 2000-04-18 Pharmaseq, Inc. Multiplex assay for nucleic acids employing transponders
US6001571A (en) 1995-11-30 1999-12-14 Mandecki; Wlodek Multiplex assay for nucleic acids employing transponders
US5709153A (en) 1995-12-29 1998-01-20 Motion Controls, Inc. High dog indexer
US6355198B1 (en) 1996-03-15 2002-03-12 President And Fellows Of Harvard College Method of forming articles including waveguides via capillary micromolding and microtransfer molding
WO1997039359A1 (en) 1996-04-15 1997-10-23 Dade International Inc. Apparatus and method for analysis
AU730633B2 (en) 1996-05-29 2001-03-08 Phillip Belgrader Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US5846727A (en) 1996-06-06 1998-12-08 Board Of Supervisors Of Louisiana State University And Agricultural & Mechanical College Microsystem for rapid DNA sequencing
DE69707288T2 (de) 1996-07-15 2002-07-18 Calcitech Ltd., Hamilton Herstellung von pulvern
US5965443A (en) 1996-09-09 1999-10-12 Wisconsin Alumni Research Foundation System for in vitro transposition
US5900481A (en) 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
US6133436A (en) 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
CA2276251A1 (en) 1996-11-20 1998-05-28 The Regents Of The University Of Michigan Microfabricated isothermal nucleic acid amplification devices and methods
US5958703A (en) 1996-12-03 1999-09-28 Glaxo Group Limited Use of modified tethers in screening compound libraries
US20020172965A1 (en) 1996-12-13 2002-11-21 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
US20050042625A1 (en) 1997-01-15 2005-02-24 Xzillion Gmbh & Co. Mass label linked hybridisation probes
US6297006B1 (en) 1997-01-16 2001-10-02 Hyseq, Inc. Methods for sequencing repetitive sequences and for determining the order of sequence subfragments
US20020034737A1 (en) 1997-03-04 2002-03-21 Hyseq, Inc. Methods and compositions for detection or quantification of nucleic acid species
IL156002A0 (en) 1997-02-12 2003-12-23 Eugene Y Chan Methods and products for analyzing polymers
US6327410B1 (en) 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US6391622B1 (en) 1997-04-04 2002-05-21 Caliper Technologies Corp. Closed-loop biochemical analyzers
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
JP2002503336A (ja) 1997-05-16 2002-01-29 アルバータ リサーチ カウンシル 微量流通システムおよびその使用方法
JP4294740B2 (ja) 1997-05-23 2009-07-15 ソレクサ・インコーポレイテッド 分析物の系列的プロセシングのためのシステムおよび装置
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
US20040241759A1 (en) 1997-06-16 2004-12-02 Eileen Tozer High throughput screening of libraries
ATE358177T1 (de) 1997-07-07 2007-04-15 Medical Res Council Ein in vitro sortierverfahren
GB9714716D0 (en) 1997-07-11 1997-09-17 Brax Genomics Ltd Characterising nucleic acids
FI103809B1 (fi) 1997-07-14 1999-09-30 Finnzymes Oy In vitro -menetelmä templaattien tuottamiseksi DNA-sekventointia varten
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
US6368871B1 (en) 1997-08-13 2002-04-09 Cepheid Non-planar microstructures for manipulation of fluid samples
EP1012335A4 (en) 1997-08-15 2004-06-09 Hyseq Inc EXPERIENCE AND COMPOSITIONS FOR DETECTING OR QUANTIFYING NUCLEIC ACID SPECIES
WO1999014368A2 (en) 1997-09-15 1999-03-25 Whitehead Institute For Biomedical Research Methods and apparatus for processing a sample of biomolecular analyte using a microfabricated device
US20020092767A1 (en) 1997-09-19 2002-07-18 Aclara Biosciences, Inc. Multiple array microfluidic device units
US7214298B2 (en) 1997-09-23 2007-05-08 California Institute Of Technology Microfabricated cell sorter
WO1999018438A1 (en) 1997-10-02 1999-04-15 Aclara Biosciences, Inc. Capillary assays involving separation of free and bound species
US5842787A (en) 1997-10-09 1998-12-01 Caliper Technologies Corporation Microfluidic systems incorporating varied channel dimensions
US6511803B1 (en) 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
JP2001519538A (ja) 1997-10-10 2001-10-23 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ 核酸アレイのレプリカ増幅
US6632526B1 (en) 1997-10-14 2003-10-14 Luminex Corporation Precision fluorescently dyed particles and methods of making and using same
EP1036332B1 (en) 1997-12-04 2005-07-13 Amersham Biosciences UK Limited Multiple assay method
AU1726199A (en) 1997-12-31 1999-07-19 Chiron Corporation Metastatic cancer regulated gene
BR9908082A (pt) 1998-02-19 2000-10-31 Harvard College Proteìna de fusão do complexo de histocompatibilidade principal classe ii, conjugado dos domìnios de ligação do complexo de histocompatibilidade principal multimérico, processos para detectar células t tendo uma especificidade do complexo definido de mhc / peptìdeo, para conferir a um indivìduo imunidade adotiva a um complexo definido de mhc / peptìdeo, para estimular ou ativar as células t reativas a um complexo definido de mhc / peptìdeo, para seletivamente matar células t reativas a um complexo definido de mhc / peptìdeo, para tolerizar um indivìduo humano a um complexo definido de mhc / peptìdeo, e, ácido nucleico isolado
AU3196099A (en) 1998-03-27 1999-10-18 President And Fellows Of Harvard College Systematic identification of essential genes by (in vitro) transposon mutagenesis
US6022716A (en) 1998-04-10 2000-02-08 Genset Sa High throughput DNA sequencing vector
EP1079967A4 (en) 1998-04-13 2003-07-23 Luminex Corp LIQUID MARKING USING FLUORESCENT MICROPARTICLES
US5997636A (en) 1998-05-01 1999-12-07 Instrumentation Technology Associates, Inc. Method and apparatus for growing crystals
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6306590B1 (en) 1998-06-08 2001-10-23 Caliper Technologies Corp. Microfluidic matrix localization apparatus and methods
WO2000008212A1 (en) 1998-08-07 2000-02-17 Cellay, Llc Gel microdrops in genetic analysis
US6159736A (en) 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
CN1326549A (zh) 1998-10-13 2001-12-12 微生物系统公司 基于无源流体动力学的流体管路元件
US6489096B1 (en) 1998-10-15 2002-12-03 Princeton University Quantitative analysis of hybridization patterns and intensities in oligonucleotide arrays
SE9803614L (sv) 1998-10-19 2000-04-20 Muhammed Mamoun Förfarande och anordning för framställning av nanopartiklar
WO2000026412A1 (en) 1998-11-02 2000-05-11 Kenneth Loren Beattie Nucleic acid analysis using sequence-targeted tandem hybridization
US5942609A (en) 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support
US6569631B1 (en) 1998-11-12 2003-05-27 3-Dimensional Pharmaceuticals, Inc. Microplate thermal shift assay for ligand development using 5-(4″dimethylaminophenyl)-2-(4′-phenyl)oxazole derivative fluorescent dyes
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
GB9900298D0 (en) 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
US6416642B1 (en) 1999-01-21 2002-07-09 Caliper Technologies Corp. Method and apparatus for continuous liquid flow in microscale channels using pressure injection, wicking, and electrokinetic injection
US6635419B1 (en) 1999-02-16 2003-10-21 Applera Corporation Polynucleotide sequencing method
US20030027214A1 (en) 1999-02-17 2003-02-06 Kamb Carl Alexander Methods for substrate-ligand interaction screening
ATE508200T1 (de) 1999-02-23 2011-05-15 Caliper Life Sciences Inc Sequenzierung durch inkorporation
US6171850B1 (en) 1999-03-08 2001-01-09 Caliper Technologies Corp. Integrated devices and systems for performing temperature controlled reactions and analyses
US6303343B1 (en) 1999-04-06 2001-10-16 Caliper Technologies Corp. Inefficient fast PCR
US6908737B2 (en) 1999-04-15 2005-06-21 Vitra Bioscience, Inc. Systems and methods of conducting multiplexed experiments
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
AU4806100A (en) 1999-04-28 2000-11-10 Board Of Trustees Of The Leland Stanford Junior University P element derived vector and methods for its use
US6399952B1 (en) 1999-05-12 2002-06-04 Aclara Biosciences, Inc. Multiplexed fluorescent detection in microfluidic devices
WO2000070095A2 (en) 1999-05-17 2000-11-23 Dade Behring Inc. Homogeneous isothermal amplification and detection of nucleic acids using a template switch oligonucleotide
US20020051971A1 (en) 1999-05-21 2002-05-02 John R. Stuelpnagel Use of microfluidic systems in the detection of target analytes using microsphere arrays
US6846622B1 (en) 1999-05-26 2005-01-25 Oregon Health & Science University Tagged epitope protein transposable element
US20030124509A1 (en) 1999-06-03 2003-07-03 Kenis Paul J.A. Laminar flow patterning and articles made thereby
US6372813B1 (en) 1999-06-25 2002-04-16 Motorola Methods and compositions for attachment of biomolecules to solid supports, hydrogels, and hydrogel arrays
WO2001002850A1 (en) 1999-07-06 2001-01-11 Caliper Technologies Corp. Microfluidic systems and methods for determining modulator kinetics
US6977145B2 (en) 1999-07-28 2005-12-20 Serono Genetics Institute S.A. Method for carrying out a biochemical protocol in continuous flow in a microreactor
US6524456B1 (en) 1999-08-12 2003-02-25 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
EP1210358A4 (en) 1999-08-13 2005-01-05 Univ Brandeis PROOF OF NUCLEIC ACIDS
EP1248853A2 (en) 1999-08-20 2002-10-16 Luminex Corporation Liquid array technology
EP1208238B1 (en) 1999-08-27 2008-11-12 Matrix Technologies Corporation Methods of immobilizing ligands on solid supports
US6982146B1 (en) 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
WO2001027610A2 (en) 1999-10-13 2001-04-19 Signature Bioscience, Inc. System and method for detecting and identifying molecular events in a test sample
US6958225B2 (en) 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
WO2001030965A2 (en) 1999-10-28 2001-05-03 The Board Of Trustees Of The Leland Stanford Junior University Methods of in vivo gene transfer using a sleeping beauty transposon system
JP4721603B2 (ja) 1999-11-08 2011-07-13 栄研化学株式会社 変異および／または多型の検出方法
US6432290B1 (en) 1999-11-26 2002-08-13 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
WO2001062887A1 (en) 2000-02-23 2001-08-30 Zyomyx, Inc. Chips having elevated sample surfaces
IL134830A0 (en) 2000-03-01 2001-05-20 Chay 13 Medical Res Group N V Peptides and immunostimulatory and anti-bacterial pharmaceutical compositions containing them
CN1487836A (zh) 2000-03-14 2004-04-07 埃米林药品公司 胰高糖素样肽1(7-36)对胃窦幽门十二指肠能动性的影响
AU5121801A (en) 2000-03-31 2001-10-15 Micronics Inc Protein crystallization in microfluidic structures
WO2001077683A1 (en) 2000-04-06 2001-10-18 Caliper Technologies Corp. Methods and devices for achieving long incubation times in high-throughput systems
DK2278029T3 (en) 2000-04-10 2016-10-03 Taxon Biosciences Inc Methods of study and genetic analysis of populations
US6481453B1 (en) 2000-04-14 2002-11-19 Nanostream, Inc. Microfluidic branch metering systems and methods
US6800298B1 (en) 2000-05-11 2004-10-05 Clemson University Biological lubricant composition and method of applying lubricant composition
US20060008799A1 (en) 2000-05-22 2006-01-12 Hong Cai Rapid haplotyping by single molecule detection
US20010048900A1 (en) 2000-05-24 2001-12-06 Bardell Ronald L. Jet vortex mixer
US6645432B1 (en) 2000-05-25 2003-11-11 President & Fellows Of Harvard College Microfluidic systems including three-dimensionally arrayed channel networks
US20060263888A1 (en) 2000-06-02 2006-11-23 Honeywell International Inc. Differential white blood count on a disposable card
US6632606B1 (en) 2000-06-12 2003-10-14 Aclara Biosciences, Inc. Methods for single nucleotide polymorphism detection
AU7299301A (en) 2000-06-21 2002-01-02 Bioarray Solutions Ltd Multianalyte molecular analysis using application-specific random particle arrays
EP1309404A2 (en) 2000-08-07 2003-05-14 Nanostream, Inc. Fluidic mixer in microfluidic system
US6610499B1 (en) 2000-08-31 2003-08-26 The Regents Of The University Of California Capillary array and related methods
US6773566B2 (en) 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
ATE448875T1 (de) 2000-09-14 2009-12-15 Caliper Life Sciences Inc Mikrofluidische vorrichtungen und methoden um temperatur-vermittelte reaktionen durchzuführen
US7294503B2 (en) 2000-09-15 2007-11-13 California Institute Of Technology Microfabricated crossflow devices and methods
EP1364052A2 (en) 2000-10-10 2003-11-26 Diversa Corporation High throughput or capillary-based screening for a bioactivity or biomolecule
JP2002155305A (ja) 2000-11-14 2002-05-31 Akira Kawasaki 単分散粒子の製造装置及び単分散粒子の製造方法及びその製造方法で製造された単分散粒子
CA2332186A1 (en) 2001-02-08 2002-08-08 Her Majesty In Right Of Canada As Represented By The Minister Of Agricul Ture And Agri-Food Canada Replicative in vivo gene targeting
US7670559B2 (en) 2001-02-15 2010-03-02 Caliper Life Sciences, Inc. Microfluidic systems with enhanced detection systems
WO2002068383A2 (en) 2001-02-22 2002-09-06 Anika Therapeutics, Inc. Thiol-modified hyaluronan
EP1721658B2 (en) 2001-02-23 2020-08-05 Japan Science and Technology Agency Process and apparatus for producing microcapsules
US20150329617A1 (en) 2001-03-14 2015-11-19 Dynal Biotech Asa Novel MHC molecule constructs, and methods of employing these constructs for diagnosis and therapy, and uses of MHC molecules
US7211654B2 (en) 2001-03-14 2007-05-01 Regents Of The University Of Michigan Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports
EP1377811B1 (en) 2001-04-03 2008-07-16 Micronics, Inc. Split focusing cytometer
US7138267B1 (en) 2001-04-04 2006-11-21 Epicentre Technologies Corporation Methods and compositions for amplifying DNA clone copy number
US20030027221A1 (en) 2001-04-06 2003-02-06 Scott Melissa E. High-throughput screening assays by encapsulation
US7572642B2 (en) 2001-04-18 2009-08-11 Ambrigen, Llc Assay based on particles, which specifically bind with targets in spatially distributed characteristic patterns
WO2002097122A1 (en) 2001-05-26 2002-12-05 One Cell Systems, Inc. Secretion of proteins by encapsulated cells
US6880576B2 (en) 2001-06-07 2005-04-19 Nanostream, Inc. Microfluidic devices for methods development
US7179423B2 (en) 2001-06-20 2007-02-20 Cytonome, Inc. Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system
US7682353B2 (en) 2001-06-29 2010-03-23 Coloplast A/S Catheter device
US6613523B2 (en) 2001-06-29 2003-09-02 Agilent Technologies, Inc. Method of DNA sequencing using cleavable tags
US7077152B2 (en) 2001-07-07 2006-07-18 Nanostream, Inc. Microfluidic metering systems and methods
US7799552B2 (en) 2001-07-20 2010-09-21 California Institute Of Technology Protein and nucleic acid expression systems
US6767731B2 (en) 2001-08-27 2004-07-27 Intel Corporation Electron induced fluorescent method for nucleic acid sequencing
US7297485B2 (en) 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
US6783647B2 (en) 2001-10-19 2004-08-31 Ut-Battelle, Llc Microfluidic systems and methods of transport and lysis of cells and analysis of cell lysate
US20030089605A1 (en) 2001-10-19 2003-05-15 West Virginia University Research Corporation Microfluidic system for proteome analysis
US20030149307A1 (en) 2001-10-24 2003-08-07 Baxter International Inc. Process for the preparation of polyethylene glycol bis amine
WO2003038558A2 (en) 2001-10-30 2003-05-08 Nanomics Biosystems Pty, Ltd. Device and methods for directed synthesis of chemical libraries
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
US7335153B2 (en) 2001-12-28 2008-02-26 Bio Array Solutions Ltd. Arrays of microparticles and methods of preparation thereof
WO2003057010A2 (en) 2002-01-04 2003-07-17 Board Of Regents, The University Of Texas System Droplet-based microfluidic oligonucleotide synthesis engine
CN1617938A (zh) 2002-01-16 2005-05-18 戴诺生物技术有限公司 从单个样品中分离核酸和蛋白质的方法
KR100459870B1 (ko) 2002-02-22 2004-12-04 한국과학기술원 트랜스포존과 Cre/loxP 부위 특이적 재조합 방법을 이용하는 염색체의 특정부위가 제거된 미생물 변이주 제조방법
CN1656234B (zh) 2002-03-20 2012-02-01 创新生物公司 包囊化核酸扩增反应混合物的微胶囊及其作为反应隔间进行平行反应的用途
ES2438008T3 (es) 2002-05-06 2014-01-15 Endocyte, Inc. Agentes de formación de imágenes dirigidos a vitaminas
EP1508044B1 (en) 2002-05-09 2010-09-01 The University of Chicago Device and method for pressure-driven plug transport and reaction
US7901939B2 (en) 2002-05-09 2011-03-08 University Of Chicago Method for performing crystallization and reactions in pressure-driven fluid plugs
US7527966B2 (en) 2002-06-26 2009-05-05 Transgenrx, Inc. Gene regulation in transgenic animals using a transposon-based vector
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
AU2003252136A1 (en) 2002-07-24 2004-02-09 Ptc Therapeutics, Inc. METHODS FOR IDENTIFYING SMALL MOLEDULES THAT MODULATE PREMATURE TRANSLATION TERMINATION AND NONSENSE MEDIATED mRNA DECAY
IL151660A0 (en) 2002-09-09 2003-04-10 Univ Ben Gurion Method for isolating and culturing unculturable microorganisms
WO2004031408A1 (en) 2002-09-30 2004-04-15 F.Hoffmann-La Roche Ag Oligonucleotides for genotyping thymidylate synthase gene
US20040081962A1 (en) 2002-10-23 2004-04-29 Caifu Chen Methods for synthesizing complementary DNA
US6979713B2 (en) 2002-11-25 2005-12-27 3M Innovative Properties Company Curable compositions and abrasive articles therefrom
US20040248299A1 (en) 2002-12-27 2004-12-09 Sumedha Jayasena RNA interference
WO2004065617A2 (en) 2003-01-17 2004-08-05 The Trustees Of Boston University Haplotype analysis
DE602004026033D1 (de) 2003-01-29 2010-04-29 454 Corp Zweiendige sequenzierung
US8420386B2 (en) 2003-02-10 2013-04-16 Max-Delbruck-Centrum Fur Molekulare Medizin (Mdc) Transposon-based targeting system
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
US7316903B2 (en) 2003-03-28 2008-01-08 United States Of America As Represented By The Department Of Health And Human Services Detection of nucleic acid sequence variations using phase Mu transposase
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
RU2541809C2 (ru) 2003-04-04 2015-02-20 Зоетис Пи ЛЛК Микрофлюидизированные эмульсии "масло в воде" и композиции вакцины
US20100035254A1 (en) 2003-04-08 2010-02-11 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
CA2521862C (en) 2003-04-10 2012-10-16 President And Fellows Of Harvard College Formation and control of fluidic species
US20060275915A1 (en) 2003-05-16 2006-12-07 Global Technologies (Nz) Ltd. Method and apparatus for mixing sample and reagent in a suspension fluid
DE112004001376D2 (de) 2003-05-19 2006-04-13 Knoell Hans Forschung Ev Vorrichtung und Verfahren zur Strukturierung von Flüssigkeiten und zum zudosieren von Reaktionsflüssigkeiten zu in Separationsmedium eingebetteten Flüssigkeitskompartimenten
WO2004105734A1 (en) 2003-05-28 2004-12-09 Valorisation Recherche, Societe En Commandite Method of preparing microcapsules
AU2004250131A1 (en) 2003-06-13 2004-12-29 The General Hospital Corporation Microfluidic systems for size based removal of red blood cells and platelets from blood
GB2403475B (en) 2003-07-01 2008-02-06 Oxitec Ltd Stable integrands
GB0315438D0 (en) 2003-07-02 2003-08-06 Univ Manchester Analysis of mixed cell populations
US8048627B2 (en) 2003-07-05 2011-11-01 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
CN1842368B (zh) 2003-08-27 2014-05-28 哈佛大学 流体物种的电子控制
JP4988345B2 (ja) 2003-09-04 2012-08-01 ザ・ユナイテッド・ステイツ・オブ・アメリカ・アズ・リプレゼンティッド・バイ・ザ・デパートメント・オブ・ヴェテランズ・アフェアーズ 眼用ハイドロゲルナノコンポジット
US7354706B2 (en) 2003-09-09 2008-04-08 The Regents Of The University Of Colorado, A Body Corporate Use of photopolymerization for amplification and detection of a molecular recognition event
US20090004739A1 (en) 2003-09-22 2009-01-01 Taku Demura Efficient Method of Preparing Dna Inverted Repeat
EP2381255A1 (en) 2003-09-25 2011-10-26 Toyama Prefecture Microwell array chip and its manufacturing method
WO2005047521A2 (en) 2003-11-10 2005-05-26 Investigen, Inc. Methods of preparing nucleic acid for detection
EP1691792A4 (en) 2003-11-24 2008-05-28 Yeda Res & Dev COMPOSITIONS AND PROCESSES FOR SORTING IN VITRO OF MOLECULE AND CELL LIBRARIES
US8071364B2 (en) 2003-12-24 2011-12-06 Transgenrx, Inc. Gene therapy using transposon-based vectors
US20050181379A1 (en) 2004-02-18 2005-08-18 Intel Corporation Method and device for isolating and positioning single nucleic acid molecules
CA2557841A1 (en) 2004-02-27 2005-09-09 President And Fellows Of Harvard College Polony fluorescent in situ sequencing beads
KR100552706B1 (ko) 2004-03-12 2006-02-20 삼성전자주식회사 핵산 증폭 방법 및 장치
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
WO2005099419A2 (en) 2004-04-13 2005-10-27 President And Fellows Of Harvard College Manipulation and/or detection of biological samples or other objects
US7799553B2 (en) 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system
US7700281B2 (en) 2004-06-30 2010-04-20 Usb Corporation Hot start nucleic acid amplification
US7968085B2 (en) 2004-07-05 2011-06-28 Ascendis Pharma A/S Hydrogel formulations
CN1648671B (zh) 2005-02-06 2012-09-26 成都夸常医学工业有限公司 多反应器分析芯片检测方法和分析芯片及检测装置
US7608434B2 (en) 2004-08-04 2009-10-27 Wisconsin Alumni Research Foundation Mutated Tn5 transposase proteins and the use thereof
WO2006030993A1 (en) 2004-09-14 2006-03-23 Jin-Ho Choy Information code system using dna sequences
US7892731B2 (en) 2004-10-01 2011-02-22 Radix Biosolutions, Ltd. System and method for inhibiting the decryption of a nucleic acid probe sequence used for the detection of a specific nucleic acid
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
WO2007001448A2 (en) 2004-11-04 2007-01-04 Massachusetts Institute Of Technology Coated controlled release polymer particles as efficient oral delivery vehicles for biopharmaceuticals
US20080004436A1 (en) 2004-11-15 2008-01-03 Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science Directed Evolution and Selection Using in Vitro Compartmentalization
US7329493B2 (en) 2004-12-22 2008-02-12 Asiagen Corporation One-tube nested PCR for detecting Mycobacterium tuberculosis
WO2006078841A1 (en) 2005-01-21 2006-07-27 President And Fellows Of Harvard College Systems and methods for forming fluidic droplets encapsulated in particles such as colloidal particles
US7579153B2 (en) 2005-01-25 2009-08-25 Population Genetics Technologies, Ltd. Isothermal DNA amplification
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
US7407757B2 (en) 2005-02-10 2008-08-05 Population Genetics Technologies Genetic analysis by sequence-specific sorting
KR101544351B1 (ko) 2005-02-18 2015-08-13 캐논 유.에스. 라이프 사이언시즈, 인크. 유기체의 게놈 ｄｎａ를 확인하기 위한 장치 및 방법
EP1867702B1 (en) 2005-02-21 2011-09-28 Kagoshima University Method for purifying biodiesel fuel
US9039273B2 (en) 2005-03-04 2015-05-26 President And Fellows Of Harvard College Method and apparatus for forming multiple emulsions
US9040237B2 (en) 2005-03-04 2015-05-26 Intel Corporation Sensor arrays and nucleic acid sequencing applications
US20070054119A1 (en) 2005-03-04 2007-03-08 Piotr Garstecki Systems and methods of forming particles
JP2006289250A (ja) 2005-04-08 2006-10-26 Kao Corp マイクロミキサー及びそれを用いた流体混合方法
CA2609328A1 (en) 2005-05-26 2006-11-30 The Trustees Of Boston University Quantification of nucleic acids and proteins using oligonucleotide mass tags
US8407013B2 (en) 2005-06-07 2013-03-26 Peter K. Rogan AB initio generation of single copy genomic probes
EP2322920B1 (en) 2005-06-07 2014-12-10 Centre National de la Recherche Scientifique (CNRS) MALDI tissue imaging using conjugates cleavable by fast fragmentation
DK1907571T3 (en) 2005-06-15 2017-08-21 Complete Genomics Inc NUCLEIC ACID ANALYSIS USING INCIDENTAL MIXTURES OF NON-OVERLAPPING FRAGMENTS
JP2006349060A (ja) 2005-06-16 2006-12-28 Ntn Corp ボールねじ
US8828209B2 (en) 2005-06-22 2014-09-09 The Research Foundation For The State University Of New York Massively parallel 2-dimensional capillary electrophoresis
WO2007002567A2 (en) 2005-06-23 2007-01-04 Nanosphere, Inc. Selective isolation and concentration of nucleic acids from complex samples
JP4927730B2 (ja) 2005-07-05 2012-05-09 一般財団法人化学及血清療法研究所 改変トランスポゾンベクター及びその利用方法
JP5051490B2 (ja) 2005-07-08 2012-10-17 独立行政法人産業技術総合研究所 マクロ生体材料を内包する無機マイクロカプセルおよびその製造方法
US20070020640A1 (en) 2005-07-21 2007-01-25 Mccloskey Megan L Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis
FR2888912B1 (fr) 2005-07-25 2007-08-24 Commissariat Energie Atomique Procede de commande d'une communication entre deux zones par electromouillage, dispositif comportant des zones isolables les unes des autres et procede de realisation d'un tel dispositif
DK1924704T3 (da) 2005-08-02 2011-09-05 Rubicon Genomics Inc Sammensætninger og fremgangsmåder til bearbejdning og mangfoldiggørelse af DNA, herunder ved anvendelse af flere enzymer i en enkelt reaktion
WO2007024840A2 (en) 2005-08-22 2007-03-01 Critical Therapeutics, Inc. Method of quantitating nucleic acids by flow cytometry microparticle-based array
JP2007074967A (ja) 2005-09-13 2007-03-29 Canon Inc 識別子プローブ及びそれを用いた核酸増幅方法
AU2006336290A1 (en) 2005-09-16 2007-07-26 The Regents Of The University Of California A colorimetric bio-barcode amplification assay for analyte detection
US7960104B2 (en) 2005-10-07 2011-06-14 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
US20070190543A1 (en) 2005-11-14 2007-08-16 Applera Corporation Coded Molecules for Detecting Target Analytes
US20070134277A1 (en) 2005-12-09 2007-06-14 Children's Medical Center Corporation Pharmaceutical formulation for sulfur-containing drugs in liquid dosage forms
DE602006008350D1 (de) 2005-12-15 2009-09-17 Akzo Nobel Coatings Int Bv Mehrlagiges beschichtungssystem
US20070141718A1 (en) 2005-12-19 2007-06-21 Bui Huy A Reduction of scan time in imaging mass spectrometry
US7932037B2 (en) 2007-12-05 2011-04-26 Perkinelmer Health Sciences, Inc. DNA assays using amplicon probes on encoded particles
WO2007081385A2 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
EP1987162A4 (en) 2006-01-23 2009-11-25 Population Genetics Technologi NUCLEIC ACID ANALYSIS ABOUT SEQUENCE TOKENS
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
WO2007089541A2 (en) 2006-01-27 2007-08-09 President And Fellows Of Harvard College Fluidic droplet coalescence
WO2007092473A2 (en) 2006-02-02 2007-08-16 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive fetal genetic screening by digital analysis
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
GB0603251D0 (en) 2006-02-17 2006-03-29 Isis Innovation DNA conformation
SG170028A1 (en) 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
CN101432439B (zh) 2006-02-24 2013-07-24 考利达基因组股份有限公司 Dna阵列上的高通量基因组测序
WO2007111937A1 (en) 2006-03-23 2007-10-04 Applera Corporation Directed enrichment of genomic dna for high-throughput sequencing
JP4921829B2 (ja) 2006-03-30 2012-04-25 株式会社東芝 微粒子の製造装置、乳化剤保持部、微粒子の製造方法および分子膜の製造方法
US20090181864A1 (en) 2006-03-31 2009-07-16 Nam Trung Nguyen Active control for droplet-based microfluidics
AU2007237909A1 (en) 2006-04-19 2007-10-25 Applied Biosystems, Llc. Reagents, methods, and libraries for gel-free bead-based sequencing
US7811603B2 (en) 2006-05-09 2010-10-12 The Regents Of The University Of California Microfluidic device for forming monodisperse lipoplexes
WO2007133710A2 (en) 2006-05-11 2007-11-22 Raindance Technologies, Inc. Microfluidic devices and methods of use thereof
EP2030011B1 (en) 2006-05-22 2017-02-01 Nanostring Technologies, Inc. Systems and methods for analyzing nanoreporters
WO2007140015A2 (en) 2006-05-26 2007-12-06 Althea Technologies, Inc Biochemical analysis of partitioned cells
FR2901717A1 (fr) 2006-05-30 2007-12-07 Centre Nat Rech Scient Procede de traitement de gouttes dans un circuit microfluidique.
AU2007260676A1 (en) 2006-06-14 2007-12-21 Artemis Health, Inc. Rare cell analysis using sample splitting and DNA tags
AU2007261445B2 (en) 2006-06-19 2013-04-18 The Johns Hopkins University Single-molecule PCR on microparticles in water-in-oil emulsions
WO2008005675A2 (en) 2006-06-30 2008-01-10 Applera Corporation Emulsion pcr and amplicon capture
EP1878501A1 (en) 2006-07-14 2008-01-16 Roche Diagnostics GmbH Instrument for heating and cooling
US9012390B2 (en) 2006-08-07 2015-04-21 Raindance Technologies, Inc. Fluorocarbon emulsion stabilizing surfactants
MX339603B (es) 2006-09-25 2016-05-31 Archer-Daniels-Midland Company Polisacaridos carboxialquilados superabsorbentes con tratamiento de la superficie y procedimientos para producir los mismos.
US7935518B2 (en) 2006-09-27 2011-05-03 Alessandra Luchini Smart hydrogel particles for biomarker harvesting
US8841116B2 (en) 2006-10-25 2014-09-23 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated DNA analysis system using same
WO2008070352A2 (en) 2006-10-27 2008-06-12 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20110045462A1 (en) 2006-11-14 2011-02-24 The Regents Of The University Of California Digital analysis of gene expression
WO2008061193A2 (en) 2006-11-15 2008-05-22 Biospherex Llc Multitag sequencing and ecogenomics analysis
US20080242560A1 (en) 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
US8598328B2 (en) 2006-12-13 2013-12-03 National University Corporation Nagoya University Tol1 factor transposase and DNA introduction system using the same
US7844658B2 (en) 2007-01-22 2010-11-30 Comcast Cable Holdings, Llc System and method for providing an application to a device
US20080176768A1 (en) 2007-01-23 2008-07-24 Honeywell Honeywell International Hydrogel microarray with embedded metal nanoparticles
EP2121983A2 (en) 2007-02-02 2009-11-25 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US8003312B2 (en) 2007-02-16 2011-08-23 The Board Of Trustees Of The Leland Stanford Junior University Multiplex cellular assays using detectable cell barcodes
FI20075124A0 (fi) 2007-02-21 2007-02-21 Valtion Teknillinen Menetelmä ja testikitti nukleotidivariaatioiden toteamiseksi
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US20080228268A1 (en) 2007-03-15 2008-09-18 Uluru, Inc. Method of Formation of Viscous, Shape Conforming Gels and Their Uses as Medical Prosthesis
EP2136786B8 (en) 2007-03-28 2012-11-14 President and Fellows of Harvard College Apparatus for forming droplets
US20100130369A1 (en) 2007-04-23 2010-05-27 Advanced Liquid Logic, Inc. Bead-Based Multiplexed Analytical Methods and Instrumentation
CN101293191B (zh) 2007-04-25 2011-11-09 中国科学院过程工程研究所 一种琼脂糖凝胶微球的制备方法
CN101720359A (zh) 2007-06-01 2010-06-02 454生命科学公司 从多重混合物中识别个别样本的系统和方法
US20100255556A1 (en) 2007-06-29 2010-10-07 President And Fellows Of Harvard College Methods and apparatus for manipulation of fluidic species
US20090068170A1 (en) 2007-07-13 2009-03-12 President And Fellows Of Harvard College Droplet-based selection
WO2009015296A1 (en) 2007-07-24 2009-01-29 The Regents Of The University Of California Microfabricated dropley generator
US20130084243A1 (en) 2010-01-27 2013-04-04 Liliane Goetsch Igf-1r specific antibodies useful in the detection and diagnosis of cellular proliferative disorders
US8563527B2 (en) 2007-08-20 2013-10-22 Pharmain Corporation Oligonucleotide core carrier compositions for delivery of nucleic acid-containing therapeutic agents, methods of making and using the same
US8268564B2 (en) 2007-09-26 2012-09-18 President And Fellows Of Harvard College Methods and applications for stitched DNA barcodes
WO2009048532A2 (en) 2007-10-05 2009-04-16 President And Fellows Of Harvard College Formation of particles for ultrasound application, drug release, and other uses, and microfluidic methods of preparation
US20090099040A1 (en) 2007-10-15 2009-04-16 Sigma Aldrich Company Degenerate oligonucleotides and their uses
US20100086914A1 (en) 2008-10-03 2010-04-08 Roche Molecular Systems, Inc. High resolution, high throughput hla genotyping by clonal sequencing
WO2009061372A1 (en) 2007-11-02 2009-05-14 President And Fellows Of Harvard College Systems and methods for creating multi-phase entities, including particles and/or fluids
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
US20110008775A1 (en) 2007-12-10 2011-01-13 Xiaolian Gao Sequencing of nucleic acids
US7771944B2 (en) 2007-12-14 2010-08-10 The Board Of Trustees Of The University Of Illinois Methods for determining genetic haplotypes and DNA mapping
JP5738597B2 (ja) 2007-12-21 2015-06-24 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸の配列決定のためのシステムおよび方法
EP4450642A3 (en) 2008-01-17 2025-01-08 Sequenom, Inc. Single molecule nucleic acid sequence analysis processes and compositions
EP2252625B1 (en) 2008-02-07 2017-12-20 Pacific Biosciences of California, Inc. Cis reactive oxygen quenchers integrated into linkers
JP5468271B2 (ja) 2008-02-08 2014-04-09 花王株式会社 微粒子分散液の製造方法
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
US9068181B2 (en) 2008-05-23 2015-06-30 The General Hospital Corporation Microfluidic droplet encapsulation
GB0810051D0 (en) 2008-06-02 2008-07-09 Oxford Biodynamics Ltd Method of diagnosis
KR20110042050A (ko) 2008-06-05 2011-04-22 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 폴리머좀, 콜로이드좀, 리포좀 및 유체 액적과 관련된 다른 종
US8198028B2 (en) 2008-07-02 2012-06-12 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces
EP2310524A4 (en) 2008-07-10 2013-07-24 Versitech Ltd METHODS OF CARTOGRAPHY OF NUCLEIC ACIDS AND IDENTIFICATION OF FINE STRUCTURAL VARIATIONS IN NUCLEIC ACIDS
US8388945B2 (en) 2008-07-11 2013-03-05 Eth Zurich Degradable microcapsules
EP4047367A1 (en) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Method for detecting target analytes with droplet libraries
WO2010009735A2 (en) 2008-07-23 2010-01-28 Dako Denmark A/S Combinatorial analysis and repair
US20100062494A1 (en) 2008-08-08 2010-03-11 President And Fellows Of Harvard College Enzymatic oligonucleotide pre-adenylation
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US20110218123A1 (en) 2008-09-19 2011-09-08 President And Fellows Of Harvard College Creation of libraries of droplets and related species
US20110159499A1 (en) 2009-11-25 2011-06-30 Quantalife, Inc. Methods and compositions for detecting genetic material
US8663920B2 (en) 2011-07-29 2014-03-04 Bio-Rad Laboratories, Inc. Library characterization by digital assay
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US20120252015A1 (en) 2011-02-18 2012-10-04 Bio-Rad Laboratories Methods and compositions for detecting genetic material
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
CN104224703A (zh) 2008-09-25 2014-12-24 赛福伦公司 苯达莫司汀的液体配制品
US8361299B2 (en) 2008-10-08 2013-01-29 Sage Science, Inc. Multichannel preparative electrophoresis system
EP2664678B1 (en) 2008-10-24 2014-10-08 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US20100113296A1 (en) 2008-11-05 2010-05-06 Joel Myerson Methods And Kits For Nucleic Acid Analysis
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
JP5685195B2 (ja) 2008-12-02 2015-03-18 バイオ−ラッド ラボラトリーズ インコーポレーティッド クロマチン構造の検出方法
EP3290531B1 (en) 2008-12-19 2019-07-24 President and Fellows of Harvard College Particle-assisted nucleic acid sequencing
KR101065807B1 (ko) 2009-01-23 2011-09-19 충남대학교산학협력단 액적 기반의 미세유체 칩을 이용한 마이크로 캡슐 제조방법
JP5457222B2 (ja) 2009-02-25 2014-04-02 エフ．ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
US9347092B2 (en) 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
WO2010104604A1 (en) 2009-03-13 2010-09-16 President And Fellows Of Harvard College Method for the controlled creation of emulsions, including multiple emulsions
KR101793744B1 (ko) 2009-03-13 2017-11-03 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 유동 포커싱 미세유동 장치의 규모 확장
EP2230312A1 (en) 2009-03-19 2010-09-22 Helmholtz-Zentrum für Infektionsforschung GmbH Probe compound for detecting and isolating enzymes and means and methods using the same
WO2010111231A1 (en) 2009-03-23 2010-09-30 Raindance Technologies, Inc. Manipulation of microfluidic droplets
US20120010091A1 (en) 2009-03-30 2012-01-12 Illumina, Inc. Gene expression analysis in single cells
CN103952482A (zh) 2009-04-02 2014-07-30 弗卢伊蒂格姆公司 用于对目标核酸进行条形码化的多引物扩增方法
FR2945545B1 (fr) 2009-05-14 2011-08-05 Univ Aix Marseille Ii Methode de detection d'adn procaryote extrait d'un echantillon de selles
FR2945819B1 (fr) 2009-05-19 2011-06-17 Commissariat Energie Atomique Dispositif et procede d'isolement de cibles biologiques ou chimiques
US8574835B2 (en) 2009-05-29 2013-11-05 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
CN102459592B (zh) 2009-06-15 2017-04-05 考利达基因组股份有限公司 用于长片段阅读测序的方法和组合物
CA2766795C (en) 2009-06-26 2017-10-03 President And Fellows Of Harvard College Fluid injection
US20110028412A1 (en) 2009-08-03 2011-02-03 Cappellos, Inc. Herbal enhanced analgesic formulations
US20110033548A1 (en) 2009-08-05 2011-02-10 E.I. Du Pont De Nemours And Company Degradable crosslinked aminated dextran microspheres and methods of use
ES2562159T3 (es) 2009-08-20 2016-03-02 Population Genetics Technologies Ltd. Composiciones y métodos para el reordenamiento de ácido nucleico molecular
BR112012004382A2 (pt) 2009-09-01 2016-03-22 Koninkl Philips Electronics Nv dispositivo para a seleção específica de moléculas-alvo, método para selecionar especificamente moléculas-alvo e uso de um dispositivo
EP2473263B1 (en) 2009-09-02 2022-11-02 President and Fellows of Harvard College Multiple emulsions created using jetting and other techniques
CA2767056C (en) 2009-09-02 2018-12-04 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
US9625454B2 (en) 2009-09-04 2017-04-18 The Research Foundation For The State University Of New York Rapid and continuous analyte processing in droplet microfluidic devices
US9714937B2 (en) 2009-10-13 2017-07-25 Nanostring Technologies, Inc. Protein detection via nanoreporters
GB0918564D0 (en) 2009-10-22 2009-12-09 Plasticell Ltd Nested cell encapsulation
EP2493619B1 (en) 2009-10-27 2018-12-19 President and Fellows of Harvard College Droplet creation techniques
WO2011056872A2 (en) 2009-11-03 2011-05-12 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
RU2573409C2 (ru) 2009-11-04 2016-01-20 Дзе Юниверсити Оф Бритиш Коламбиа Содержащие нуклеиновые кислоты липидные частицы и относящиеся к ним способы
GB2485850C (en) 2009-11-25 2019-01-23 Bio Rad Laboratories Methods and compositions for detecting copy number and chromosome aneuploidy by ligation probes and partitioning the ligated products prior to amplification
US9023769B2 (en) 2009-11-30 2015-05-05 Complete Genomics, Inc. cDNA library for nucleic acid sequencing
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
EP2513333B1 (en) 2009-12-17 2013-10-02 Keygene N.V. Restriction enzyme based whole genome sequencing
EP2517025B1 (en) 2009-12-23 2019-11-27 Bio-Rad Laboratories, Inc. Methods for reducing the exchange of molecules between droplets
US8889416B2 (en) 2010-01-21 2014-11-18 California Institute Of Technology Methods and devices for micro-isolation, extraction, and/or analysis of microscale components
JP5901046B2 (ja) 2010-02-19 2016-04-06 国立大学法人 千葉大学 ＯＡＴＰ１Ｂ３ｍＲＮＡの新規な選択的スプライシングバリアント
JP2013524171A (ja) 2010-03-25 2013-06-17 クァンタライフ・インコーポレーテッド 液滴ベースのアッセイのための液滴の発生
KR101866401B1 (ko) 2010-04-05 2018-06-11 프로그노시스 바이오사이언스, 인코포레이티드 공간적으로 엔코딩된 생물학적 검정
US20110287947A1 (en) * 2010-05-18 2011-11-24 University Of Southern California Tethered Conformation Capture
US20120000777A1 (en) 2010-06-04 2012-01-05 The Regents Of The University Of California Devices and methods for forming double emulsion droplet compositions and polymer particles
US8703493B2 (en) 2010-06-15 2014-04-22 Src, Inc. Location analysis using fire retardant-protected nucleic acid-labeled tags
WO2012003374A2 (en) 2010-07-02 2012-01-05 The Board Of Trustees Of The Leland Stanford Junior University Targeted sequencing library preparation by genomic dna circularization
AU2011274642B2 (en) * 2010-07-09 2015-05-21 Cergentis B.V. 3-d genomic region of interest sequencing strategies
US20120238738A1 (en) 2010-07-19 2012-09-20 New England Biolabs, Inc. Oligonucleotide Adapters: Compositions and Methods of Use
CN103202812B (zh) 2010-08-09 2015-10-28 南京大学 一种制备用于体内递送药理活性物质的蛋白纳米粒的方法
EP2616103A1 (en) 2010-09-16 2013-07-24 The University of North Carolina At Chapel Hill Asymmetric bifunctional silyl monomers and particles thereof as prodrugs and delivery vehicles for pharmaceutical, chemical and biological agents
EP2619327B1 (en) 2010-09-21 2014-10-22 Population Genetics Technologies LTD. Increasing confidence of allele calls with molecular counting
EP2625526B1 (en) 2010-10-04 2017-03-15 Genapsys Inc. Systems and methods for automated reusable parallel biological reactions
US9999886B2 (en) 2010-10-07 2018-06-19 The Regents Of The University Of California Methods and systems for on demand droplet generation and impedance based detection
EP3561073A1 (en) 2010-10-08 2019-10-30 President and Fellows of Harvard College High-throughput immune sequencing
US9902950B2 (en) 2010-10-08 2018-02-27 President And Fellows Of Harvard College High-throughput single cell barcoding
US20130225623A1 (en) 2010-10-27 2013-08-29 Mount Sinai School Of Medicine Methods of Treating Psychiatric or Neurological Disorders with MGLUR Antagonists
CA2821299C (en) 2010-11-05 2019-02-12 Frank J. Steemers Linking sequence reads using paired code tags
US8829171B2 (en) 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
WO2012083225A2 (en) 2010-12-16 2012-06-21 Gigagen, Inc. System and methods for massively parallel analysis of nycleic acids in single cells
US8765455B2 (en) 2011-01-27 2014-07-01 Lawrence Livermore National Security, Llc Chip-based droplet sorting
GB201101429D0 (en) 2011-01-27 2011-03-16 Biocompatibles Uk Ltd Drug delivery system
EP2668273B9 (en) 2011-01-28 2017-03-29 Illumina, Inc. Oligonucleotide replacement for di-tagged and directional libraries
JP6017458B2 (ja) 2011-02-02 2016-11-02 ユニヴァーシティ・オブ・ワシントン・スルー・イッツ・センター・フォー・コマーシャリゼーション 大量並列連続性マッピング
EP3483285B1 (en) * 2011-02-09 2021-07-14 Bio-Rad Laboratories, Inc. Analysis of nucleic acids
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
US9260753B2 (en) 2011-03-24 2016-02-16 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
GB2489714B (en) 2011-04-05 2013-11-06 Tracesa Ltd Fluid Identification Method
WO2012150317A1 (en) 2011-05-05 2012-11-08 Institut National De La Sante Et De La Recherche Medicale (Inserm) Linear dna amplification
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
JP2014512826A (ja) * 2011-04-25 2014-05-29 バイオ−ラド ラボラトリーズ インコーポレイテッド 核酸分析のための方法および組成物
SG10201900183VA (en) 2011-04-28 2019-05-30 Univ Leland Stanford Junior Identification of polynucleotides associated with a sample
US9957558B2 (en) 2011-04-28 2018-05-01 Life Technologies Corporation Methods and compositions for multiplex PCR
WO2012157684A1 (ja) 2011-05-16 2012-11-22 地方独立行政法人 大阪府立病院機構 血中ｄｎａの定量的検出による悪性新生物の病勢の進行を評価する方法
BR112013029729A2 (pt) 2011-05-23 2017-01-24 Basf Se controle de emulsões, incluindo emulsões múltiplas
US9005935B2 (en) 2011-05-23 2015-04-14 Agilent Technologies, Inc. Methods and compositions for DNA fragmentation and tagging by transposases
CN103890191B (zh) 2011-05-27 2018-12-04 哈佛大学校长及研究员协会 单细胞全基因组扩增方法
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
US9150916B2 (en) 2011-06-24 2015-10-06 Beat Christen Compositions and methods for identifying the essential genome of an organism
US8927218B2 (en) 2011-06-27 2015-01-06 Flir Systems, Inc. Methods and compositions for segregating target nucleic acid from mixed nucleic acid samples
EP2729501A2 (en) 2011-07-07 2014-05-14 Life Technologies Corporation Polymer particles, nucleic acid polymer particles and methods of making and using the same
US20130017978A1 (en) 2011-07-11 2013-01-17 Finnzymes Oy Methods and transposon nucleic acids for generating a dna library
US9605304B2 (en) 2011-07-20 2017-03-28 The Hong Kong Polytechnic University Ultra-stable oligonucleotide-gold and-silver nanoparticle conjugates and method of their preparation
US20130189700A1 (en) 2011-07-25 2013-07-25 Bio-Rad Laboratories, Inc. Breakage of an emulsion containing nucleic acid
HK1199745A1 (en) 2011-09-09 2015-07-17 The Board Of Trustees Of The Leland Stanford Junior University Methods for obtaining a sequence
US9514272B2 (en) 2011-10-12 2016-12-06 Complete Genomics, Inc. Identification of DNA fragments and structural variations
US9469874B2 (en) 2011-10-18 2016-10-18 The Regents Of The University Of California Long-range barcode labeling-sequencing
US20130109576A1 (en) 2011-10-28 2013-05-02 Anthony P. Shuber Methods for detecting mutations
EP2774277A4 (en) 2011-11-04 2015-07-22 Intel Corp SIGNALING FOR THE CONFIGURATION OF COORDINATED DOWNLINK MULTIPORT COMMUNICATIONS
WO2013072790A1 (en) 2011-11-16 2013-05-23 International Business Machines Corporation Microfluidic device with deformable valve
US10689643B2 (en) 2011-11-22 2020-06-23 Active Motif, Inc. Targeted transposition for use in epigenetic studies
US9938524B2 (en) 2011-11-22 2018-04-10 Active Motif, Inc. Multiplex isolation of protein-associated nucleic acids
EP2794928B1 (en) 2011-12-22 2019-02-20 President and Fellows of Harvard College Compositions and methods for analyte detection
US20150125865A1 (en) 2011-12-23 2015-05-07 Gigagen, Inc. Methods And Apparatuses For Droplet Mixing
CN104245745B (zh) 2012-02-09 2017-03-29 生命技术公司 亲水性聚合物颗粒及其制备方法
CN104271768A (zh) 2012-02-14 2015-01-07 约翰霍普金斯大学 miRNA分析方法
WO2013123382A1 (en) 2012-02-15 2013-08-22 Wisconsin Alumni Research Foundation Dithioamine reducing agents
US10202628B2 (en) 2012-02-17 2019-02-12 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
US9176031B2 (en) 2012-02-24 2015-11-03 Raindance Technologies, Inc. Labeling and sample preparation for sequencing
EP2823064B1 (en) 2012-03-05 2019-02-06 President and Fellows of Harvard College Methods for epigenetic sequencing
NO2694769T3 (enExample) 2012-03-06 2018-03-03
EP2647426A1 (en) 2012-04-03 2013-10-09 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Replication of distributed nucleic acid molecules with preservation of their relative distribution through hybridization-based binding
BR112014025695B1 (pt) 2012-04-16 2021-08-03 Biological Dynamics, Inc Preparação de amostra de ácido nucleico
US20130296173A1 (en) 2012-04-23 2013-11-07 Complete Genomics, Inc. Pre-anchor wash
WO2013177220A1 (en) 2012-05-21 2013-11-28 The Scripps Research Institute Methods of sample preparation
JP6558830B2 (ja) 2012-06-15 2019-08-14 ボード・オブ・リージエンツ，ザ・ユニバーシテイ・オブ・テキサス・システム 複数転写産物のハイスループットシークエンシング
EP2881465B1 (en) * 2012-07-30 2018-07-04 Hitachi, Ltd. Tag-sequence-attached two-dimensional cdna library device, and gene expression analysis method and gene expression analysis apparatus each utilizing same
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20140378322A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
CN114891871A (zh) 2012-08-14 2022-08-12 10X基因组学有限公司 微胶囊组合物及方法
US20140378345A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US20140378349A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US20150005200A1 (en) 2012-08-14 2015-01-01 10X Technologies, Inc. Compositions and methods for sample processing
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20150005199A1 (en) 2012-08-14 2015-01-01 10X Technologies, Inc. Compositions and methods for sample processing
WO2014047561A1 (en) 2012-09-21 2014-03-27 The Broad Institute Inc. Compositions and methods for labeling of agents
US9644199B2 (en) 2012-10-01 2017-05-09 Agilent Technologies, Inc. Immobilized transposase complexes for DNA fragmentation and tagging
GB201217772D0 (en) 2012-10-04 2012-11-14 Base4 Innovation Ltd Sequencing method
WO2014062717A1 (en) 2012-10-15 2014-04-24 Life Technologies Corporation Compositions, methods, systems and kits for target nucleic acid enrichment
EP3901280B1 (en) 2012-10-17 2025-03-12 10x Genomics Sweden AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
CN104869993B (zh) 2012-10-25 2019-01-15 通用医疗公司 治疗阿尔茨海默病及相关疾病的组合疗法
EP2914745B1 (en) 2012-11-05 2017-09-06 Rubicon Genomics, Inc. Barcoding nucleic acids
WO2014074611A1 (en) 2012-11-07 2014-05-15 Good Start Genetics, Inc. Methods and systems for identifying contamination in samples
CN105026576A (zh) 2012-12-03 2015-11-04 以琳生物药物有限公司 单链多核苷酸扩增方法
MX380562B (es) 2012-12-12 2025-03-12 Broad Inst Inc Modificaciones de sistemas, métodos y composiciones guía optimizadas para la manipulación de secuencias.
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP2752664A1 (en) 2013-01-07 2014-07-09 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Label-free method for the detection of analytes
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
KR20230003659A (ko) 2013-02-08 2023-01-06 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
SG10201710049RA (en) 2013-03-08 2018-01-30 Bio Rad Laboratories Inc Compositions, methods and systems for polymerase chain reaction assays
EP2970951B1 (en) 2013-03-13 2019-02-20 Illumina, Inc. Methods for nucleic acid sequencing
US9273349B2 (en) 2013-03-14 2016-03-01 Affymetrix, Inc. Detection of nucleic acids
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
CA2907050C (en) 2013-03-15 2023-09-26 Prognosys Biosciences, Inc. Methods for detecting peptide/mhc/tcr binding
WO2014145078A1 (en) 2013-03-15 2014-09-18 Verinata Health, Inc. Generating cell-free dna libraries directly from blood
US20140274729A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Methods, compositions and kits for generation of stranded rna or dna libraries
WO2014144495A1 (en) 2013-03-15 2014-09-18 Abvitro, Inc. Single cell bar-coding for antibody discovery
EP2970959B1 (en) 2013-03-15 2018-05-30 Lineage Biosciences, Inc. Methods and compositions for tagging and analyzing samples
EP2971080B1 (en) 2013-03-15 2018-01-03 Expedeon, S.L. Methods for amplification and sequencing using thermostable tthprimpol
US9896717B2 (en) 2013-05-09 2018-02-20 Bio-Rad Laboratories, Inc. Magnetic immuno digital PCR assay
US10059989B2 (en) 2013-05-23 2018-08-28 The Board Of Trustees Of The Leland Stanford Junior University Transposition of native chromatin for personal epigenomics
US20160122753A1 (en) 2013-06-12 2016-05-05 Tarjei Mikkelsen High-throughput rna-seq
JP6600302B2 (ja) 2013-06-12 2019-10-30 ザ ジェネラル ホスピタル コーポレイション 標的分子のマルチプレックス検出のための方法、キット、およびシステム、ならびにそれらの使用
JP6738728B2 (ja) 2013-06-17 2020-08-19 ザ・ブロード・インスティテュート・インコーポレイテッド 肝臓ターゲティングおよび治療のためのＣＲＩＳＰＲ−Ｃａｓ系、ベクターおよび組成物の送達および使用
US20160208323A1 (en) 2013-06-21 2016-07-21 The Broad Institute, Inc. Methods for Shearing and Tagging DNA for Chromatin Immunoprecipitation and Sequencing
CN111500680B (zh) 2013-06-25 2024-04-16 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统
KR102366116B1 (ko) 2013-06-27 2022-02-23 10엑스 제노믹스, 인크. 샘플 처리를 위한 조성물 및 방법
EP3019618B1 (en) 2013-07-12 2018-10-31 University of South Alabama Minimal piggybac vectors for genome integration
CN105705659B (zh) 2013-08-28 2019-11-29 贝克顿迪金森公司 大规模平行单细胞分析
CN105764490B (zh) 2013-09-24 2020-10-09 加利福尼亚大学董事会 用于生物测定和诊断的胶囊封装的传感器和感测系统及其制造和使用方法
GB201317301D0 (en) 2013-09-30 2013-11-13 Linnarsson Sten Method for capturing and encoding nucleic acid from a plurality of single cells
WO2015065924A2 (en) 2013-10-28 2015-05-07 Massachusetts Institute Of Technology Hydrogel microstructures with immiscible fluid isolation for small reaction volumes
GB201320351D0 (en) * 2013-11-18 2014-01-01 Erasmus Universiteit Medisch Ct Method
US9834814B2 (en) * 2013-11-22 2017-12-05 Agilent Technologies, Inc. Spatial molecular barcoding of in situ nucleic acids
CN105765080A (zh) 2013-11-26 2016-07-13 伯乐实验室公司 用于检测核酸相邻性的方法
US11091758B2 (en) * 2013-12-11 2021-08-17 The Regents Of The University Of California Methods for labeling DNAa fragments to reconstruct physical linkage and phase
US20140315755A1 (en) 2013-12-26 2014-10-23 Tao Chen Genome-wide Antisense Oligonucleotide and RNAi
JP6608368B2 (ja) 2013-12-30 2019-11-20 アトレカ インコーポレイテッド 核酸バーコードを用いた単一細胞と関連づけられた核酸の分析方法
WO2015113725A1 (en) 2014-02-03 2015-08-06 Thermo Fisher Scientific Baltics Uab Method for controlled dna fragmentation
EP2986761B1 (en) * 2014-02-13 2018-08-15 Bio-rad Laboratories, Inc. Chromosome conformation capture in droplet partitions
PL3110975T3 (pl) 2014-02-27 2021-12-13 Jumpcode Genomics, Inc. Metody analizy somatycznych elementów ruchomych i ich zastosowania
US9694361B2 (en) 2014-04-10 2017-07-04 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
CN106507677B (zh) 2014-04-15 2021-03-12 伊鲁米那股份有限公司 用于改进插入序列偏倚和增加dna输入耐受性的修饰的转座酶
EP3134536B1 (en) 2014-04-21 2019-12-18 President and Fellows of Harvard College Systems and methods for barcoding nucleic acids
US10975371B2 (en) 2014-04-29 2021-04-13 Illumina, Inc. Nucleic acid sequence analysis from single cells
EP3146046B1 (en) 2014-05-23 2020-03-11 Digenomix Corporation Haploidome determination by digitized transposons
JP5949832B2 (ja) 2014-05-30 2016-07-13 ダイキン工業株式会社 空調システム
AU2015271324B2 (en) 2014-06-06 2019-09-12 Herlev Hospital Determining antigen recognition through barcoding of MHC multimers
WO2015191877A1 (en) 2014-06-11 2015-12-17 Life Technologies Corporation Systems and methods for substrate enrichment
EP4270006A3 (en) 2014-06-13 2024-01-10 Immudex ApS General detection and isolation of specific cells by binding of labeled molecules
CN114214314A (zh) 2014-06-24 2022-03-22 生物辐射实验室股份有限公司 数字式pcr条码化
EP3161161A4 (en) 2014-06-26 2018-02-28 10X Genomics, Inc. Methods and compositions for sample analysis
KR20170026383A (ko) 2014-06-26 2017-03-08 10엑스 제노믹스, 인크. 핵산 서열의 분석
EP4574974A3 (en) 2014-06-26 2025-10-08 10x Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
EP4235677A3 (en) 2014-06-26 2023-11-22 10X Genomics, Inc. Processes and systems for nucleic acid sequence assembly
US20160024558A1 (en) 2014-07-23 2016-01-28 10X Genomics, Inc. Nucleic acid binding proteins and uses thereof
JP2017532024A (ja) 2014-09-09 2017-11-02 ザ・ブロード・インスティテュート・インコーポレイテッド コンポジット単一細胞核酸分析のための液滴ベースの方法および機器
US10590483B2 (en) 2014-09-15 2020-03-17 Abvitro Llc High-throughput nucleotide library sequencing
BR122021026779B1 (pt) 2014-10-17 2023-12-19 Illumina Cambridge Limited Transposon de preservação de contiguidade
JP2017532042A (ja) 2014-10-29 2017-11-02 １０エックス ゲノミクス，インコーポレイテッド 標的化核酸配列決定のための方法及び組成物
AU2016207023B2 (en) 2015-01-12 2019-12-05 10X Genomics, Inc. Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
CN107530654A (zh) 2015-02-04 2018-01-02 加利福尼亚大学董事会 通过在离散实体中条形码化对核酸进行测序
EP4286516A3 (en) 2015-02-24 2024-03-06 10X Genomics, Inc. Partition processing methods and systems
AU2016222719B2 (en) 2015-02-24 2022-03-31 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
WO2016160908A1 (en) 2015-03-30 2016-10-06 Verily Life Sciences Llc Methods for combining single cell profiling with combinatorial nanoparticle conjugate library screening
EP4321627A3 (en) 2015-04-10 2024-04-17 10x Genomics Sweden AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
CN107709574B (zh) 2015-04-14 2021-10-01 皇家飞利浦有限公司 生物组织样品的分子概况的空间作图
EP3298168A4 (en) 2015-05-18 2019-02-20 10X Genomics, Inc. STABILIZED REDUCING AGENTS AND METHOD FOR THEIR USE
KR20180008493A (ko) 2015-05-18 2018-01-24 10엑스 제노믹스, 인크. 생화학 반응 및 분석에 사용하기 위한 이동성 고체상 조성물
WO2016187717A1 (en) 2015-05-26 2016-12-01 Exerkine Corporation Exosomes useful for genome editing
WO2016191618A1 (en) 2015-05-27 2016-12-01 Jianbiao Zheng Methods of inserting molecular barcodes
JP2018527016A (ja) 2015-06-24 2018-09-20 オックスフォード バイオダイナミックス リミテッド 染色体相互作用の検出
US10894980B2 (en) 2015-07-17 2021-01-19 President And Fellows Of Harvard College Methods of amplifying nucleic acid sequences mediated by transposase/transposon DNA complexes
ES2779798T3 (es) 2015-08-12 2020-08-19 Cemm - Forschungszentrum Für Molekulare Medizin Gmbh Métodos para estudiar ácidos nucleicos
KR20180085717A (ko) 2015-09-24 2018-07-27 에이비비트로, 엘엘씨 친화성-올리고뉴클레오타이드 콘쥬게이트 및 그것의 사용
FR3042658B1 (fr) 2015-10-15 2017-10-27 Renault Sas Dispositif de gestion thermique d'un groupe motopropulseur electrique.
WO2017075294A1 (en) 2015-10-28 2017-05-04 The Board Institute Inc. Assays for massively combinatorial perturbation profiling and cellular circuit reconstruction
US11092607B2 (en) 2015-10-28 2021-08-17 The Board Institute, Inc. Multiplex analysis of single cell constituents
US20170145476A1 (en) 2015-11-19 2017-05-25 10X Genomics, Inc. Transformable tagging compositions, methods, and processes incorporating same
KR102723865B1 (ko) 2015-12-04 2024-10-29 10엑스 제노믹스, 인크. 핵산 분석을 위한 방법 및 조성물
US20170260584A1 (en) 2016-02-11 2017-09-14 10X Genomics, Inc. Cell population analysis using single nucleotide polymorphisms from single cell transcriptomes
WO2017156336A1 (en) 2016-03-10 2017-09-14 The Board Of Trustees Of The Leland Stanford Junior University Transposase-mediated imaging of the accessible genome
WO2018044831A1 (en) 2016-08-30 2018-03-08 Integrated Dna Technologies, Inc. Cleavable hairpin primers
GB2569252A (en) 2016-08-31 2019-06-12 Harvard College Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing
WO2018075693A1 (en) 2016-10-19 2018-04-26 10X Genomics, Inc. Methods and systems for barcoding nucleic acid molecules from individual cells or cell populations
GB201619458D0 (en) 2016-11-17 2017-01-04 Spatial Transcriptomics Ab Method for spatial tagging and analysing nucleic acids in a biological specimen
CN118441026A (zh) 2016-12-19 2024-08-06 生物辐射实验室股份有限公司 液滴加标的相邻性保留的标签化dna
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4310183B1 (en) 2017-01-30 2025-07-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding

Also Published As

Publication number Publication date
DE202016009134U1 (de) 2022-09-02
EP3882357B1 (en) 2022-08-10
US10774370B2 (en) 2020-09-15
KR20240161696A (ko) 2024-11-12
US20210238660A1 (en) 2021-08-05
EP4524255C0 (en) 2026-05-13
US20210246490A1 (en) 2021-08-12
ES2926495T3 (es) 2022-10-26
US20240167079A1 (en) 2024-05-23
EP3384048A1 (en) 2018-10-10
EP4782550A3 (en) 2026-08-05
JP2022000050A (ja) 2022-01-04
CN108431232B (zh) 2022-10-14
EP4524255B1 (en) 2026-05-13
EP4524255A3 (en) 2025-04-02
EP4524255A2 (en) 2025-03-19
US11473125B2 (en) 2022-10-18
JP6954899B2 (ja) 2021-10-27
JP7182679B2 (ja) 2022-12-02
IL259736A (en) 2018-07-31
EP4782550A2 (en) 2026-07-29
EP3882357A1 (en) 2021-09-22
US20170159109A1 (en) 2017-06-08
EP3384048B1 (en) 2021-03-24
US11624085B2 (en) 2023-04-11
US11873528B2 (en) 2024-01-16
EP4144861B1 (en) 2024-09-11
US20190085380A1 (en) 2019-03-21
DK3882357T3 (da) 2022-08-29
US12421539B2 (en) 2025-09-23
US20260049345A1 (en) 2026-02-19
SG11201804086VA (en) 2018-06-28
WO2017096158A1 (en) 2017-06-08
CN115369161A (zh) 2022-11-22
SG10202108763UA (en) 2021-09-29
JP2018537086A (ja) 2018-12-20
KR102723865B1 (ko) 2024-10-29
CN108431232A (zh) 2018-08-21
EP4144861A1 (en) 2023-03-08
CN115369161B (zh) 2026-03-06
EP4144861C0 (en) 2024-09-11
KR20180081164A (ko) 2018-07-13
US20230073186A1 (en) 2023-03-09

Similar Documents

Publication Publication Date Title
IL259736A (en) Methods and systems for nucleic acid analysis
PT3325669T (pt) Composições e métodos de análise de rna
IL249618A0 (en) Methods and preparations for analyzing samples
SG10201912283RA (en) Nucleic acid sequence analysis from single cells
BR112017024747A2 (pt) composições e métodos para enriquecer populações de ácidos nucleicos
SI3334841T1 (sl) Postopki za proučevanje nukleinskih kislin
PT3108009T (pt) Métodos e composições para a criação de perfis de adn
GB201516569D0 (en) Methods, compositions, and kits for nucleic acid analysis
SG10202007321RA (en) Sample preparation for nucleic acid amplification
SG10201913820TA (en) Nucleic Acid Analysis Apparatus
GB2548733B (en) Nucleic acid analysis device
GB201403216D0 (en) Nucleic acid sample preparation
GB201405226D0 (en) Nucleic acid preparation method
SG10201600867XA (en) Nucleic acid extracting device
GB201518379D0 (en) Analytical method and composition
GB201413318D0 (en) Nucleic acid sample preparation
GB201418621D0 (en) Improved nucleic acid sample preparation using concatenation
GB201513128D0 (en) Nucleic acid detection method
IL260122A (en) Methods and compositions for assembly of nucleic acids
GB201506669D0 (en) Nucleic acid sample enrichment
GB201415674D0 (en) Nucleic acid analysis
GB2553907B (en) Nucleic acid analyzer
GB201519323D0 (en) Nucleic acid analysis
GB201503845D0 (en) Nucleic acid analysis
ZA201705645B (en) Methods and compositions for dna profiling