MX391104B - Glucosiltransferasas modificadas con ingeniería genética y métodos de glucosilación de glucósido de esteviol. - Google Patents

Glucosiltransferasas modificadas con ingeniería genética y métodos de glucosilación de glucósido de esteviol.

Info

Publication number
MX391104B
MX391104B MX2019009233A MX2019009233A MX391104B MX 391104 B MX391104 B MX 391104B MX 2019009233 A MX2019009233 A MX 2019009233A MX 2019009233 A MX2019009233 A MX 2019009233A MX 391104 B MX391104 B MX 391104B
Authority
MX
Mexico
Prior art keywords
enzymes
methods
present
rebaudioside
polypeptides
Prior art date
Application number
MX2019009233A
Other languages
English (en)
Spanish (es)
Other versions
MX2019009233A (es
Inventor
Courtney Dianne Moffett
David Entwistle
Jack Liang
Jonathan Vroom
Joyce Liu
Nikki Dellas
Stephanie Sue Galanie
Original Assignee
Tate & Lyle Solutions Usa Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tate & Lyle Solutions Usa Llc filed Critical Tate & Lyle Solutions Usa Llc
Publication of MX2019009233A publication Critical patent/MX2019009233A/es
Publication of MX391104B publication Critical patent/MX391104B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1062—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01013—Sucrose synthase (2.4.1.13)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
MX2019009233A 2017-02-03 2018-02-01 Glucosiltransferasas modificadas con ingeniería genética y métodos de glucosilación de glucósido de esteviol. MX391104B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762454417P 2017-02-03 2017-02-03
US201762479262P 2017-03-30 2017-03-30
PCT/US2018/016359 WO2018144679A2 (en) 2017-02-03 2018-02-01 Engineered glycosyltransferases and steviol glycoside glucosylation methods

Publications (2)

Publication Number Publication Date
MX2019009233A MX2019009233A (es) 2019-09-19
MX391104B true MX391104B (es) 2025-03-21

Family

ID=63038689

Family Applications (4)

Application Number Title Priority Date Filing Date
MX2019009233A MX391104B (es) 2017-02-03 2018-02-01 Glucosiltransferasas modificadas con ingeniería genética y métodos de glucosilación de glucósido de esteviol.
MX2022003824A MX2022003824A (es) 2017-02-03 2019-08-02 Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol.
MX2021005861A MX2021005861A (es) 2017-02-03 2019-08-02 Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol.
MX2021005862A MX2021005862A (es) 2017-02-03 2019-08-02 Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol.

Family Applications After (3)

Application Number Title Priority Date Filing Date
MX2022003824A MX2022003824A (es) 2017-02-03 2019-08-02 Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol.
MX2021005861A MX2021005861A (es) 2017-02-03 2019-08-02 Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol.
MX2021005862A MX2021005862A (es) 2017-02-03 2019-08-02 Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol.

Country Status (15)

Country Link
US (5) US20180223264A1 (enExample)
EP (3) EP3878967A3 (enExample)
JP (4) JP7248302B2 (enExample)
KR (3) KR20260006703A (enExample)
CN (2) CN110914445B (enExample)
AU (4) AU2018215335B2 (enExample)
BR (1) BR112019015992A2 (enExample)
CA (3) CA3050310A1 (enExample)
IL (3) IL268121B2 (enExample)
MX (4) MX391104B (enExample)
MY (1) MY199186A (enExample)
PH (1) PH12019501698A1 (enExample)
SG (1) SG11201906480QA (enExample)
TW (3) TWI797103B (enExample)
WO (2) WO2018144679A2 (enExample)

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MY202423A (en) * 2017-03-06 2024-04-28 Conagen Inc Biosynthetic production of steviol glycoside rebaudioside d4 from rebaudioside e
AU2019304965B2 (en) * 2018-07-16 2025-04-24 Manus Bio, Inc. Production of steviol glycosides through whole cell biotransformation
IL319918A (en) 2018-07-30 2025-05-01 Tate & Lyle Solutions Usa Llc Engineered glycosyltransferases and methods for glycosylation of steviol glycoside
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KR102546737B1 (ko) * 2020-11-25 2023-06-22 씨제이제일제당 주식회사 포도당 전이 스테비아를 포함하는 감미질이 개선된 조성물
CN114480326B (zh) * 2021-01-08 2024-04-26 中国科学院华南植物园 亚精胺衍生物糖基转移酶LbUGT73及其编码基因和应用
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CN115678867B (zh) * 2021-07-27 2023-12-01 弈柯莱生物科技(集团)股份有限公司 一种蔗糖合成酶及其应用
CN113462670B (zh) * 2021-08-23 2023-03-24 兴化格林生物制品有限公司 一种糖基转移酶突变体及其催化合成莱鲍迪苷m的方法
WO2023044368A1 (en) * 2021-09-17 2023-03-23 Doublerainbow Biosciences Inc. Method for producing glycosylated therapeutics by using an immobilized enzyme preparation
KR102628374B1 (ko) * 2021-10-19 2024-01-24 씨제이제일제당 주식회사 신규한 당전이효소 및 이의 용도
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CN118489006A (zh) * 2021-12-24 2024-08-13 株式会社三养社 糖基转移酶变体和使用其制备甜菊糖苷的方法
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CN120112655A (zh) * 2022-10-26 2025-06-06 阿泽达公司 甜菊醇糖苷的酶促介导的反应性结晶
CN120858181A (zh) 2022-12-05 2025-10-28 泰特和莱尔解决方案美国公司 获得甜菊醇糖苷的方法
CN116426506B (zh) * 2023-03-10 2024-01-30 云南师范大学 低温活性提高的β-木糖苷酶突变体D259G及其应用
CN122459446A (zh) * 2023-06-06 2026-07-24 阿泽达公司 用于制备莱鲍迪苷d和莱鲍迪苷m的组合物和方法
CN119432785B (zh) * 2023-08-07 2026-02-06 中国科学院天津工业生物技术研究所 一种高丝氨酸脱氢酶突变体及其应用
CN117106685A (zh) * 2023-08-17 2023-11-24 江南大学 一种利用糖基转移酶高效生物合成莱鲍迪苷m2的方法
WO2025039672A1 (zh) 2023-08-24 2025-02-27 弈柯莱生物科技(集团)股份有限公司 莱鲍迪苷M的制备方法和β-1,2-葡萄糖基转移酶在其中的应用
CN119931981B (zh) * 2024-05-29 2026-05-05 东台市浩瑞生物科技有限公司 一种糖基转移酶ugt76g1突变体及其催化合成莱鲍迪苷a的方法
WO2026063396A1 (ja) * 2024-09-20 2026-03-26 富士フイルム株式会社 変異型スクロースシンターゼ、変異型スクロースシンターゼをコードする核酸、発現ベクター、形質転換体、変異型スクロースシンターゼの製造方法、変異型スクロースシンターゼを含む組成物、ウリジン二リン酸－グルコースの製造方法、糖化合物の製造方法
WO2026101250A1 (ko) * 2024-11-06 2026-05-15 씨제이제일제당 (주) 신규한 당전이 효소 및 이의 용도
CN120384061B (zh) * 2025-04-23 2026-03-13 杭州力文所生物科技有限公司 一种用于催化生产莱鲍迪苷m的糖基转移酶及其重组基因
CN121718520B (zh) * 2026-02-26 2026-06-26 山东奔月生物科技股份有限公司 β-1,3-糖基转移酶突变体及其在催化合成莱鲍迪苷M中的应用

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