BR112019015992A2 - Glicosiltransferase engenheirada, polinucleotídeo engenheirado, vetor, célula hospedeira, métodos para produzir pelo menos uma glicosiltransferase engenheirada, para produzir pelo menos uma variante de sacarose sintase engenheirada, para produzir rebaudiosídeo m, rebaudiosídeo a e/ou rebaudiosídeo i e rebaudiosídeo d e para glicosilação de um substrato, composição, sacarose sintase engenheirada, rebaudiosídeo m, rebaudiosídeo a, rebaudiosídeo i, e, rebaudiosídeo d. - Google Patents

Glicosiltransferase engenheirada, polinucleotídeo engenheirado, vetor, célula hospedeira, métodos para produzir pelo menos uma glicosiltransferase engenheirada, para produzir pelo menos uma variante de sacarose sintase engenheirada, para produzir rebaudiosídeo m, rebaudiosídeo a e/ou rebaudiosídeo i e rebaudiosídeo d e para glicosilação de um substrato, composição, sacarose sintase engenheirada, rebaudiosídeo m, rebaudiosídeo a, rebaudiosídeo i, e, rebaudiosídeo d. Download PDF

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Publication number
BR112019015992A2
BR112019015992A2 BR112019015992-4A BR112019015992A BR112019015992A2 BR 112019015992 A2 BR112019015992 A2 BR 112019015992A2 BR 112019015992 A BR112019015992 A BR 112019015992A BR 112019015992 A2 BR112019015992 A2 BR 112019015992A2
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BR
Brazil
Prior art keywords
rebaudioside
engineered
glycosyltransferase
produce
methods
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BR112019015992-4A
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English (en)
Portuguese (pt)
Inventor
Vroom Jonathan
Sue GALANIE Stephanie
DELLAS Nikki
Liang Jack
Liu Joyce
Entwistle David
Dianne MOFFETT Courtney
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Codexis, Inc.
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Publication of BR112019015992A2 publication Critical patent/BR112019015992A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1062—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01013—Sucrose synthase (2.4.1.13)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
BR112019015992-4A 2017-02-03 2018-02-01 Glicosiltransferase engenheirada, polinucleotídeo engenheirado, vetor, célula hospedeira, métodos para produzir pelo menos uma glicosiltransferase engenheirada, para produzir pelo menos uma variante de sacarose sintase engenheirada, para produzir rebaudiosídeo m, rebaudiosídeo a e/ou rebaudiosídeo i e rebaudiosídeo d e para glicosilação de um substrato, composição, sacarose sintase engenheirada, rebaudiosídeo m, rebaudiosídeo a, rebaudiosídeo i, e, rebaudiosídeo d. BR112019015992A2 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201762454417P 2017-02-03 2017-02-03
US62/454,417 2017-02-03
US201762479262P 2017-03-30 2017-03-30
US62/479,262 2017-03-30
PCT/US2018/016359 WO2018144679A2 (en) 2017-02-03 2018-02-01 Engineered glycosyltransferases and steviol glycoside glucosylation methods

Publications (1)

Publication Number Publication Date
BR112019015992A2 true BR112019015992A2 (pt) 2020-05-26

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BR112019015992-4A BR112019015992A2 (pt) 2017-02-03 2018-02-01 Glicosiltransferase engenheirada, polinucleotídeo engenheirado, vetor, célula hospedeira, métodos para produzir pelo menos uma glicosiltransferase engenheirada, para produzir pelo menos uma variante de sacarose sintase engenheirada, para produzir rebaudiosídeo m, rebaudiosídeo a e/ou rebaudiosídeo i e rebaudiosídeo d e para glicosilação de um substrato, composição, sacarose sintase engenheirada, rebaudiosídeo m, rebaudiosídeo a, rebaudiosídeo i, e, rebaudiosídeo d.

Country Status (15)

Country Link
US (5) US20180223264A1 (enExample)
EP (3) EP3878967A3 (enExample)
JP (4) JP7248302B2 (enExample)
KR (3) KR20260006703A (enExample)
CN (2) CN118956802A (enExample)
AU (4) AU2018215335B2 (enExample)
BR (1) BR112019015992A2 (enExample)
CA (3) CA3175100A1 (enExample)
IL (3) IL304833B2 (enExample)
MX (4) MX391104B (enExample)
MY (1) MY199186A (enExample)
PH (1) PH12019501698A1 (enExample)
SG (1) SG11201906480QA (enExample)
TW (3) TW202237834A (enExample)
WO (2) WO2018144675A1 (enExample)

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WO2020237226A1 (en) * 2019-05-23 2020-11-26 Arzeda Corp. Compositions and methods for producing steviol glycosides
KR102709594B1 (ko) * 2019-06-25 2024-09-26 마누스 바이오, 인크. 우리딘 다이포스페이트-의존성 글라이코실트랜스퍼라제 효소
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JP2024520118A (ja) 2021-06-01 2024-05-21 エーバイオケム バイオテクノロジー（グループ）カンパニー，リミティド 糖転移酵素及びその使用
CN115449514B (zh) * 2021-06-08 2023-09-29 弈柯莱生物科技(上海)股份有限公司 一种β-1,2-糖基转移酶及其应用
CN115678867B (zh) * 2021-07-27 2023-12-01 弈柯莱生物科技(集团)股份有限公司 一种蔗糖合成酶及其应用
CN113462670B (zh) * 2021-08-23 2023-03-24 兴化格林生物制品有限公司 一种糖基转移酶突变体及其催化合成莱鲍迪苷m的方法
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KR102628374B1 (ko) * 2021-10-19 2024-01-24 씨제이제일제당 주식회사 신규한 당전이효소 및 이의 용도
KR102924285B1 (ko) * 2021-12-24 2026-02-09 주식회사 삼양사 당전이 효소 변이체 및 이를 이용한 스테비올 배당체의 제조방법
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CN116426506B (zh) * 2023-03-10 2024-01-30 云南师范大学 低温活性提高的β-木糖苷酶突变体D259G及其应用
WO2024254112A1 (en) * 2023-06-06 2024-12-12 Arzeda Corp. Compositions and methods for producing rebaudioside d and rebaudioside m
CN119432785B (zh) * 2023-08-07 2026-02-06 中国科学院天津工业生物技术研究所 一种高丝氨酸脱氢酶突变体及其应用
WO2025039672A1 (zh) 2023-08-24 2025-02-27 弈柯莱生物科技(集团)股份有限公司 莱鲍迪苷M的制备方法和β-1,2-葡萄糖基转移酶在其中的应用
CN119931981B (zh) * 2024-05-29 2026-05-05 东台市浩瑞生物科技有限公司 一种糖基转移酶ugt76g1突变体及其催化合成莱鲍迪苷a的方法
WO2026063396A1 (ja) * 2024-09-20 2026-03-26 富士フイルム株式会社 変異型スクロースシンターゼ、変異型スクロースシンターゼをコードする核酸、発現ベクター、形質転換体、変異型スクロースシンターゼの製造方法、変異型スクロースシンターゼを含む組成物、ウリジン二リン酸－グルコースの製造方法、糖化合物の製造方法
CN120384061B (zh) * 2025-04-23 2026-03-13 杭州力文所生物科技有限公司 一种用于催化生产莱鲍迪苷m的糖基转移酶及其重组基因
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B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]