KR101021387B1 - 아데노바이러스 벡터를 생산하기 위한 수단 및 방법 - Google Patents
아데노바이러스 벡터를 생산하기 위한 수단 및 방법 Download PDFInfo
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- KR101021387B1 KR101021387B1 KR1020047016954A KR20047016954A KR101021387B1 KR 101021387 B1 KR101021387 B1 KR 101021387B1 KR 1020047016954 A KR1020047016954 A KR 1020047016954A KR 20047016954 A KR20047016954 A KR 20047016954A KR 101021387 B1 KR101021387 B1 KR 101021387B1
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- Prior art keywords
- adenovirus
- serotype
- orf6
- coding sequence
- derived
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Abstract
Description
Claims (31)
- 제1 혈청형의 아데노바이러스의 구조적 및 비-구조적 요소들을 포함하는 재조합 아데노바이러스 벡터로, 상기 벡터는 기능적 E4-orf6 단백질을 암호화하는 서열을 추가로 포함하고, 여기서 상기 서열은a) 상기 제1 혈청형과 다른 제2 혈청형의 아데노바이러스로부터 유래된 E4-orf6 암호화 서열; 및b) 상기 제1 혈청형과 다른 제2 혈청형으로부터 유래된 E4-orf6 암호화 서열의 일부와, 제3 혈청형으로부터 유래된 E4-orf6 암호화 서열의 일부 사이의 융합물을 포함하는 (여기서 상기 제3 혈청형은 상기 제1 혈청형과 동일하거나 또는 다를 수 있다) E4-orf6 암호화 서열로 이루어진 군으로부터 선택되는 것인 재조합 아데노바이러스 벡터.
- 제 1항에 있어서, 상기 제1 혈청형과 상기 제2 혈청형이 다른 아데노바이러스 서브그룹으로부터 유래된 재조합 아데노바이러스 벡터.
- 제 1항에 있어서, 상기 제1 혈청형이 서브그룹 C 이외의 서브그룹으로부터 유래되고, 상기 E4-orf6 암호화 서열은 서브그룹 C의 아데노바이러스 혈청형으로부터 유래된 것인 재조합 아데노바이러스 벡터.
- 제 1항에 있어서, 상기 제1 혈청형은 서브그룹 B로부터 유래되고, 상기 제2 혈청형은 서브그룹 C로부터 유래된 재조합 아데노바이러스 벡터.
- 제 1항에 있어서, 상기 E4-orf6 암호화 서열은 아데노바이러스 혈청형 5로부터 유래된 재조합 아데노바이러스 벡터.
- 제 1항 내지 제 5항 중 어느 하나의 항에 있어서, 상기 제1 혈청형이 아데노바이러스 혈청형 11, 14, 16, 21, 34, 35 및 50으로 이루어진 군으로부터 선택되는 것인 재조합 아데노바이러스 벡터.
- 제 1항 내지 제 5항 중 어느 하나의 항에 있어서, 비-아데노바이러스 단백질, 폴리펩티드 또는 펩티드를 암호화하는 서열을 추가로 포함하는 재조합 아데노바이러스 벡터.
- 제 7항에 있어서, 상기 비-아데노바이러스 단백질, 폴리펩티드 또는 펩티드는; 세포-치사 유도 폴리펩티드, 병원성 유기체의 항원성 결정기, 종양 특이 항원, 바이러스성 단백질, 호르몬 및 시토킨으로 이루어진 군으로부터 선택되는 재조합 아데노바이러스 벡터.
- 제1 혈청형의 아데노바이러스의 구조적 및 비-구조적 요소들을 포함하는 재조합 아데노바이러스 벡터의 생산방법으로, 상기 방법은a) 발현 형태로 제2 혈청형의 아데노바이러스로부터 유래된, E1B-55K 암호화 서열을 정착시키고 있는 보충 세포에, 상기 보충 세포에 의한 상기 재조합 아데노바이러스 벡터의 조립을 가능하게 하는 아데노바이러스의 필수 요소들을 제공하는 단계, 여기서 상기 요소들은 상기 제2 혈청형과 다른 상기 제1 혈청형의 아데노바이러스로부터의 몇몇 이상의 구조적 및 비-구조적 요소들 및 (상기 보충 세포의 상기 발현가능한 E1B-55K 단백질과 양립할 수 있는) 기능적 E4-orf6 단백질의 암호화 서열을 포함하고;b) 상기 보충 세포를 아데노바이러스 벡터의 생산 및 조립이 일어나는 것을 가능하게 하는 조건하의 배지에서 배양시키는 단계; 및c) 그렇게 생산된 재조합 아데노바이러스를 배지 및/또는 보충 세포로부터 수확하는 단계로 이루어지며,여기서 상기 양립가능한 E4-orf6 단백질을 암호화하는 서열은 그렇게 생산된 재조합 아데노바이러스 벡터에 존재하는 방법.
- 제 9항에 있어서, 상기 E4-orf6 암호화 서열이a) 상기 제2 혈청형의 아데노바이러스로부터 유래된 E4-orf6 암호화 서열;b) 상기 제1 및 제2 혈청형과 다른 제3 혈청형의 아데노바이러스로부터 유래된 E4-orf6 암호화 서열; 및c) 제3 혈청형으로부터 유래된 E4-orf6 암호화 서열의 일부(여기서 상기 제3 혈청형은 상기 제1 혈청형과 동일하거나 또는 다르다)와 상기 제2 혈청형의 아데노바이러스로부터 유래된 E4-orf6 암호화 서열의 일부 사이의 융합물을 포함하는 E4-orf6 암호화 서열로 이루어진 군으로부터 선택되는 것인 방법.
- 제 9항 또는 제 10항에 있어서, 상기 제1 및 제2 혈청형은 다른 서브그룹으로부터 유래된 방법.
- 제 9항 또는 제 10항에 있어서, 상기 제2 혈청형이 서브그룹 C의 아데노바이러스 혈청형인 방법.
- 제 12항에 있어서, 상기 제2 혈청형이 아데노바이러스 혈청형 5인 방법.
- 제 9항 또는 제 10항에 있어서, 상기 제1 혈청형이 서브그룹 B의 아데노바이러스 혈청형인 방법.
- 제 14항에 있어서, 상기 제1 혈청형이 아데노바이러스 혈청형 11, 14, 16, 21, 34, 35 및 50으로 이루어진 군으로부터 선택되는 방법.
- 제 9항 또는 제 10항에 있어서, 상기 E1B-55K 암호화 서열이 상기 보충 세포의 게놈으로 통합되어 있는 방법.
- 제 9항 또는 제 10항에 있어서, 상기 보충 세포가 1차 이배체 인간 세포 또는 그의 자손 세포로부터 유도되는 방법.
- 제 9항 또는 제 10항에 있어서, 상기 보충 세포가 1차 인간 망막아세포, 1차 인간 배아 신장 세포, 1차 인간 신경세포 또는 일차 인간 양수 아세포로부터 유도되는 방법.
- 제 9항 또는 제 10항에 있어서, 상기 보충 세포가, 그의 게놈으로 통합된, 하나 이상의 아데노바이러스 E1A 단백질을 암호화하는 핵산을 포함하는 방법.
- 제 19항에 있어서, 상기 하나 이상의 아데노바이러스 E1A 단백질을 암호화하는 핵산이 서브그룹 B와 다른 서브그룹의 아데노바이러스 혈청형으로부터 유래되는 방법.
- 제 19항에 있어서, 상기 하나 이상의 아데노바이러스 E1A 단백질을 암호화하는 핵산이 서브그룹C의 아데노바이러스 혈청형으로부터 유래되는 방법.
- 제 20항에 있어서, 상기 하나 이상의 아데노바이러스 E1A 단백질 암호화 핵산이 아데노바이러스 혈청형 5로부터 유래되는 방법.
- 제 9항 또는 제 10항에 있어서, 상기 E4-orf6 암호화 서열 및 상기 E1B-55K 암호화 서열이 다른 아데노바이러스 혈청형들로부터 유래되고 여기서 상기 다른 아데노바이러스 혈청형들이 동일한 아데노바이러스 서브그룹의 멤버인 방법.
- 제 9항 또는 제 10항에 있어서, 상기 E4-orf6 암호화 서열 및 상기 E1B-55K 암호화 서열이 다른 아데노바이러스 혈청형들로부터 유래되고 여기서 상기 다른 아데노바이러스 혈청형들은 둘 다 서브그룹 C의 멤버인 방법.
- 제 9항 또는 제 10항에 있어서, 상기 E4-orf6 암호화 서열 및 상기 E1B-55K 암호화 서열이 동일한 아데노바이러스 혈청형으로부터 유래되는 방법.
- 제 25항에 있어서, 상기 E4-orf6 암호화 서열 및 상기 E1B-55K 암호화 서열이 아데노바이러스 혈청형 5로부터 유래되는 방법.
- 제 9항에 있어서, 상기 보충 세포가 PER.C6 세포 또는 그의 유도체인 방법.
- 삭제
- a) 제1 혈청형의 아데노바이러스의 구조적 및 비-구조적 요소들을 포함하는 재조합 아데노바이러스 벡터의 생산을 위한 보충 세포, (여기서 상기 세포는 발현가능한 형태의 제2 혈청형의 아데노바이러스로부터 유래된, E1B-55K 암호화 서열을 정착시키고 있다), 및b) 상기 보충 세포에 의한 상기 재조합 아데노바이러스 벡터의 조립을 가능하게 하는 모든 필수 아데노바이러스 요소들을 포함하는 하나 이상의 복제가능한 핵산 벡터로, 여기서 상기 요소들은 상기 제2 혈청형과 다른 상기 제1 혈청형의 아데노바이러스로부터 유래된 몇몇 이상의 구조적 및 비-구조적 요소들 및 상기 보충 세포중의 상기 발현가능한 E1B-55K 단백질과 양립할 수 있는 기능적 E4-orf6 단백질을 암호화하는 서열을 포함하는 것인 키트.
- 제 29항에 있어서, 상기 E4-orf6 암호화 서열은:a) 상기 제2 혈청형의 아데노바이러스로부터 유래된 E4-orf6 암호화 서열;b) 상기 제1 및 제2 혈청형과 다른 제3 혈청형의 아데노바이러스로부터 유래된 E4-orf6 암호화 서열; 및c) 제3 혈청형으로부터 유래된 E4-orf6 암호화 서열의 일부(여기서 상기 제3 혈청형은 상기 제1 혈청혈과 동일하거나 또는 다르다)와 상기 제2 혈청형의 아데노바이러스로부터 유래된 E4-orf6 암호화 서열의 일부 사이의 융합물을 포함하는 E4-orf6 암호화 서열로 이루어진 군으로부터 선택되는 것인 키트.
- 삭제
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
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NLPCT/NL02/00280 | 2002-04-25 | ||
NL0200280 | 2002-04-25 | ||
PCT/EP2003/050125 WO2003104467A1 (en) | 2002-04-25 | 2003-04-24 | Means and methods for the production of adenovirus vectors |
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KR20040106363A KR20040106363A (ko) | 2004-12-17 |
KR101021387B1 true KR101021387B1 (ko) | 2011-03-14 |
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KR1020047016954A KR101021387B1 (ko) | 2002-04-25 | 2003-04-24 | 아데노바이러스 벡터를 생산하기 위한 수단 및 방법 |
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US (2) | US7468181B2 (ko) |
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