JP7370529B2 - 多能性幹細胞製造システム、幹細胞の誘導方法、幹細胞の浮遊培養方法、幹細胞の浮遊培養器、人工多能性幹細胞の作製方法、及び動物細胞から特定の体細胞を作製する方法 - Google Patents
多能性幹細胞製造システム、幹細胞の誘導方法、幹細胞の浮遊培養方法、幹細胞の浮遊培養器、人工多能性幹細胞の作製方法、及び動物細胞から特定の体細胞を作製する方法 Download PDFInfo
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| JP2022063287A JP2022088643A (ja) | 2015-08-31 | 2022-04-06 | 多能性幹細胞製造システム、幹細胞の誘導方法、幹細胞の浮遊培養方法、幹細胞の浮遊培養器、人工多能性幹細胞の作製方法、及び動物細胞から特定の体細胞を作製する方法 |
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Families Citing this family (54)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11286454B2 (en) | 2015-08-31 | 2022-03-29 | I Peace, Inc. | Pluripotent stem cell manufacturing system and method for producing induced pluripotent stem cells |
| CA3044251A1 (en) | 2016-11-18 | 2018-05-24 | New York Stem Cell Foundation, Inc. | Microfluidic system and method of use thereof |
| EP4039792A1 (en) | 2017-01-20 | 2022-08-10 | FUJIFILM Corporation | Cell culture apparatus and cell culture method |
| EP3587556A4 (en) * | 2017-02-24 | 2021-04-14 | Koji Tanabe | CELL TREATMENT DEVICE, SUSPENSION CULTURE VESSEL AND STEM CELL INDUCTION METHOD |
| WO2018155595A1 (ja) * | 2017-02-24 | 2018-08-30 | 剛士 田邊 | 人工多能性幹細胞の作製方法 |
| EP3530727A4 (en) * | 2017-02-27 | 2020-07-08 | Koji Tanabe | SOMATIC CELL PRODUCTION SYSTEM |
| CN115247129A (zh) * | 2017-02-27 | 2022-10-28 | 田边刚士 | 细胞处理装置 |
| AU2018272017B2 (en) * | 2017-05-26 | 2021-11-04 | Kite Pharma, Inc. | Methods of making and using embryonic mesenchymal progenitor cells |
| CN111065728B (zh) * | 2017-08-24 | 2023-12-12 | 泰尔茂比司特公司 | 细胞扩增 |
| SG11202002047QA (en) * | 2017-09-08 | 2020-04-29 | Otsuka Pharma Factory Inc | Stem cell derived from neonatal pig and method for producing same |
| WO2019131626A1 (ja) * | 2017-12-28 | 2019-07-04 | オリンパス株式会社 | 細胞培養制御方法、細胞培養制御装置、細胞培養装置および細胞培養システム |
| US12252682B2 (en) | 2018-02-09 | 2025-03-18 | Global Life Sciences Solutions Usa Llc | System and method for fluid flow management in a bioprocessing system |
| US11932842B2 (en) | 2018-02-09 | 2024-03-19 | Global Life Sciences Solutions Usa Llc | Bioprocessing apparatus |
| US11920119B2 (en) | 2018-02-09 | 2024-03-05 | Global Life Sciences Solutions Usa Llc | Systems and methods for bioprocessing |
| EP3749741A1 (en) * | 2018-02-09 | 2020-12-16 | Global Life Sciences Solutions USA LLC | Apparatus for fluid line management in a bioprocessing system |
| US12077743B2 (en) | 2018-02-09 | 2024-09-03 | Global Life Sciences Solutions Usa Llc | Apparatus for fluid line management in a bioprocessing system |
| JP6939643B2 (ja) * | 2018-02-26 | 2021-09-22 | 株式会社Ihi | 細胞分割装置及び細胞培養装置 |
| EP3810749A4 (en) * | 2018-06-19 | 2022-04-20 | Stemcell Technologies Canada Inc. | SYSTEMS, METHODS AND APPARATUS FOR AUTOMATED CELL CULTURE |
| US11948307B2 (en) * | 2018-07-17 | 2024-04-02 | National University Corporation Kobe University | Solid-liquid boundary detection device and pre-processing device provided with same |
| EP3778860A4 (en) * | 2018-07-24 | 2021-05-26 | FUJIFILM Corporation | OPERATING PROGRAM FOR CELL CULTURE SUPPORT DEVICE, CELL CULTURE SUPPORT DEVICE AND OPERATING PROCEDURES FOR A CELL CULTURE SUPPORT DEVICE |
| JP7280574B2 (ja) * | 2018-08-20 | 2023-05-24 | アイ ピース，インコーポレイテッド | 細胞培養器 |
| CN112601813A (zh) * | 2018-08-20 | 2021-04-02 | 田边刚士 | 细胞的培养或诱导方法 |
| WO2020044984A1 (ja) * | 2018-08-27 | 2020-03-05 | 富士フイルム株式会社 | 細胞培養方法及び細胞培養装置 |
| US20200248123A1 (en) | 2019-02-05 | 2020-08-06 | Corning Incorporated | Packed-bed bioreactor systems and methods of using the same |
| JP2020167959A (ja) * | 2019-04-04 | 2020-10-15 | オリンパス株式会社 | 培地モニタリング装置 |
| JP7416764B2 (ja) * | 2019-03-25 | 2024-01-17 | テルモ株式会社 | 細胞培養物を清浄度の高い空間において調製するための容器及びキット |
| WO2020196649A1 (ja) * | 2019-03-27 | 2020-10-01 | 株式会社カネカ | 細胞製造装置、細胞製造方法並びにこれに用いられるサーバ、システムおよび装置 |
| CN110066733A (zh) * | 2019-05-29 | 2019-07-30 | 广东唯泰生物科技有限公司 | 干细胞培养机器人 |
| CN113785049B (zh) * | 2019-06-10 | 2025-11-25 | 爱平世股份有限公司 | 细胞培养方法及单核细胞的回收方法 |
| EP3992274A4 (en) * | 2019-06-28 | 2023-08-09 | I Peace, Inc. | CELL GROUPS DIVIDER, METHOD FOR MAKING CELL GROUPS DIVIDER AND METHOD FOR DIVIDING CELL GROUPS |
| US20220251492A1 (en) * | 2019-06-28 | 2022-08-11 | I Peace, Inc. | Cell culture vessel and cell culture device |
| CN110229751A (zh) * | 2019-07-17 | 2019-09-13 | 中国人民解放军军事科学院军事医学研究院 | mRNA制备系统及制备方法 |
| US20230037137A1 (en) * | 2019-08-29 | 2023-02-02 | Fanuc Corporation | Cell production device and system therefor |
| JP7297272B2 (ja) * | 2019-08-29 | 2023-06-26 | ファナック株式会社 | 細胞製造装置 |
| JP7391339B2 (ja) * | 2019-08-29 | 2023-12-05 | ファナック株式会社 | 細胞製造装置及びその製造方法 |
| US11118151B2 (en) | 2019-11-05 | 2021-09-14 | Corning Incorporated | Fixed bed bioreactor and methods of using the same |
| US20220403307A1 (en) * | 2019-11-06 | 2022-12-22 | I Peace, Inc. | Cell culture device |
| CN111126329A (zh) * | 2019-12-30 | 2020-05-08 | 杭州原生生物科技有限公司 | 一种自动识别多能干细胞群的方法 |
| WO2021166227A1 (ja) * | 2020-02-21 | 2021-08-26 | アイ ピース， インコーポレイテッド | 溶液搬送装置 |
| CA3178792A1 (en) * | 2020-05-22 | 2021-11-25 | Sqz Biotechnologies Company | Point of care system for automatically processing cells |
| CN111666895B (zh) * | 2020-06-08 | 2023-05-26 | 上海市同济医院 | 基于深度学习的神经干细胞分化方向预测系统及方法 |
| JP7682619B2 (ja) * | 2020-10-19 | 2025-05-26 | キヤノンメディカルシステムズ株式会社 | 誘導多能性幹細胞樹立装置及び方法 |
| US20240084262A1 (en) * | 2020-12-23 | 2024-03-14 | Cedars-Sinai Medical Center | Methods for differentiation of pancreatic exocrine cells from human induced pluripotent stem cells |
| JP7744155B2 (ja) * | 2021-04-19 | 2025-09-25 | キヤノンメディカルシステムズ株式会社 | 多能性幹細胞製造システム |
| JP7664656B2 (ja) * | 2021-05-18 | 2025-04-18 | 国立大学法人京都大学 | 多能性幹細胞の製造方法 |
| CN113265333A (zh) * | 2021-06-23 | 2021-08-17 | 无锡生基医药科技有限公司 | 一种全封闭、半自动化控制、模块化的质粒瞬时转染系统 |
| WO2023034720A1 (en) * | 2021-08-28 | 2023-03-09 | Cornell University | Compositions and methods for cell reprogramming |
| WO2023053220A1 (ja) * | 2021-09-28 | 2023-04-06 | 公益財団法人京都大学ｉＰＳ細胞研究財団 | 多能性幹細胞の製造方法 |
| EP4423237A2 (en) * | 2021-10-27 | 2024-09-04 | Regenerative Research Foundation | Cell culture feeding device |
| JP2024085747A (ja) * | 2022-12-15 | 2024-06-27 | ヤマハ発動機株式会社 | 組換えタンパク質産生細胞の培養方法 |
| WO2024176440A1 (ja) * | 2023-02-24 | 2024-08-29 | 公益財団法人京都大学ｉＰＳ細胞研究財団 | 人工多能性幹細胞の製造方法 |
| CN116426573A (zh) * | 2023-04-14 | 2023-07-14 | 臻赫医药(杭州)有限公司 | 重编程因子抗衰老表达系统、生物材料及用途 |
| JP7721836B1 (ja) * | 2023-12-04 | 2025-08-12 | 公益財団法人京都大学ｉＰＳ細胞研究財団 | 人工多能性幹細胞の製造方法 |
| CN118460359B (zh) * | 2024-07-10 | 2024-09-20 | 江苏颐泽生物科技有限公司 | 一种具有细胞活性检测功能的干细胞贮存装置 |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005521405A (ja) | 2002-03-28 | 2005-07-21 | ブラスティコン ビオテクノロギッシュ フォーシュング ゲーエムベーハー | 単球を起源に持つ、脱分化したプログラム可能な幹細胞およびそれらの製造と使用 |
| WO2009096614A1 (en) | 2008-01-30 | 2009-08-06 | Mirae Biotech Co., Ltd. | Method of manufacturing induced pluripotent stem cell originated from somatic cell |
| JP2012528599A (ja) | 2009-06-05 | 2012-11-15 | セルラー ダイナミクス インターナショナル， インコーポレイテッド | リプログラミングｔ細胞および造血細胞 |
| WO2014017513A1 (ja) | 2012-07-24 | 2014-01-30 | 日産化学工業株式会社 | 培地組成物及び当該組成物を用いた細胞又は組織の培養方法 |
Family Cites Families (67)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0258795B1 (en) * | 1986-08-27 | 1993-11-03 | Kawasumi Laboratories, Inc. | A method for cultivating cells and an instrument therefor |
| US5399493A (en) * | 1989-06-15 | 1995-03-21 | The Regents Of The University Of Michigan | Methods and compositions for the optimization of human hematopoietic progenitor cell cultures |
| DE69634794T2 (de) * | 1995-03-20 | 2006-04-27 | PRECISION SYSTEM SCIENCE Co., Ltd., Matsudo | Verfahren und gerät für die flüssigkeitsbehandlung mittels eines verteilers |
| CN1609593B (zh) | 1996-08-16 | 2012-04-25 | Ge保健尼亚加拉公司 | 用于分析井板、凝胶和斑点的数字成像系统 |
| JP4309815B2 (ja) | 2004-07-05 | 2009-08-05 | 株式会社日立製作所 | 細胞培養システム及び細胞培養方法 |
| AU2005322159A1 (en) | 2004-12-29 | 2006-07-06 | Biogen Idec Ma Inc. | Bioreactor process control system and method |
| CN100335612C (zh) * | 2005-01-20 | 2007-09-05 | 上海交通大学 | 植物细胞或组织两段连续培养系统 |
| CN1664545A (zh) * | 2005-03-18 | 2005-09-07 | 宁波美生医疗器材有限公司 | 一种细胞薄层涂片的制作方法 |
| JP2007000038A (ja) | 2005-06-22 | 2007-01-11 | Toray Ind Inc | 閉鎖系循環回路型培養装置 |
| CN2841667Y (zh) * | 2005-09-09 | 2006-11-29 | 刘平 | 细胞滤过器 |
| US20070087437A1 (en) * | 2005-10-14 | 2007-04-19 | Jifan Hu | Methods for rejuvenating cells in vitro and in vivo |
| EA018039B1 (ru) | 2005-12-13 | 2013-05-30 | Киото Юниверсити | Ядерный фактор перепрограммирования |
| EP2155857B1 (en) * | 2007-05-04 | 2013-05-29 | Erik Sundström | Slicing device |
| JP2008307007A (ja) * | 2007-06-15 | 2008-12-25 | Bayer Schering Pharma Ag | 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞 |
| WO2009006422A1 (en) | 2007-06-29 | 2009-01-08 | Stem Cell Products, Inc. | Automated method and apparatus for embryonic stem cell culture |
| US7858371B2 (en) * | 2007-09-26 | 2010-12-28 | Weyerhaeuser Nr Company | Method of separating embryo suspension mass |
| US9683232B2 (en) * | 2007-12-10 | 2017-06-20 | Kyoto University | Efficient method for nuclear reprogramming |
| US9339818B2 (en) | 2008-01-09 | 2016-05-17 | Screencell | Device and method for isolating and cultivating live cells on a filter or extracting the genetic material thereof |
| WO2010017562A2 (en) * | 2008-08-08 | 2010-02-11 | Mayo Foundation For Medical Education And Research | Induced pluripotent stem cells |
| EP2192174B1 (en) * | 2008-11-21 | 2015-11-11 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Reprogramming cells toward a pluripotent state |
| WO2010141320A1 (en) | 2009-06-01 | 2010-12-09 | Mariposa Biotechnology, Inc. | Device for removing cumulus from oocytes |
| CN102686717B (zh) * | 2009-10-09 | 2014-10-29 | 佐治亚科技研究公司 | 用于分散生物材料聚结体的方法和设备 |
| CA3174901A1 (en) * | 2009-11-12 | 2011-05-19 | Technion Research & Development Foundation Ltd. | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
| EP2528687B1 (en) * | 2010-01-29 | 2018-08-22 | Micronics, Inc. | System comprising a host instrument and a microfluidic cartridge for assays |
| US8802438B2 (en) * | 2010-04-16 | 2014-08-12 | Children's Medical Center Corporation | Compositions, kits, and methods for making induced pluripotent stem cells using synthetic modified RNAs |
| WO2011142813A1 (en) * | 2010-05-12 | 2011-11-17 | Cellectis Sa | Dynamic mixing and electroporation chamber and system |
| US8048675B1 (en) * | 2010-05-12 | 2011-11-01 | Ipierian, Inc. | Integration-free human induced pluripotent stem cells from blood |
| EP2614141B1 (en) * | 2010-09-07 | 2019-07-31 | Technion Research & Development Foundation Limited | Novel methods and culture media for culturing pluripotent stem cells |
| US20160272934A1 (en) * | 2010-10-08 | 2016-09-22 | Cellanyx Diagnostics, Llc | Systems, devices and methods for microfluidic culturing, manipulation and analysis of tissues and cells |
| CN102559587A (zh) * | 2010-12-16 | 2012-07-11 | 中国科学院上海药物研究所 | 诱导性多能干细胞的制备方法以及用于制备诱导性多能干细胞的培养基 |
| US9732319B2 (en) * | 2010-12-22 | 2017-08-15 | Fate Therapeutics, Inc. | Cell culture platform for single cell sorting and enhanced reprogramming of iPSCs |
| CN102174395B (zh) | 2011-01-30 | 2013-08-21 | 中国科学院广州生物医药与健康研究院 | 诱导多能干细胞自动化扩增与培养系统 |
| WO2012115153A1 (ja) | 2011-02-25 | 2012-08-30 | 株式会社ニコン | 細胞評価方法、細胞培養方法、細胞評価装置、インキュベータ、細胞評価プログラム、コロニー分類プログラム、幹細胞の培養方法、幹細胞評価装置および幹細胞評価プログラム |
| GB201105413D0 (en) * | 2011-03-30 | 2011-05-11 | Cambridge Entpr Ltd | Nuclear reprogramming substrate |
| CA2833026A1 (en) * | 2011-04-15 | 2012-10-18 | The University Of British Columbia | Method and apparatus for separation of particles |
| PL2768942T3 (pl) * | 2011-10-17 | 2020-05-18 | Massachusetts Institute Of Technology | Dostarczanie dokomórkowe |
| US20140248698A1 (en) | 2011-10-21 | 2014-09-04 | Arkray, Inc. | Method for culturing pluripotency-maintained singly dispersed cells by means of laminar flow |
| EP2594632A1 (en) * | 2011-11-18 | 2013-05-22 | Miltenyi Biotec GmbH | Method and device for cell modification |
| WO2013077423A1 (ja) * | 2011-11-25 | 2013-05-30 | 国立大学法人京都大学 | 多能性幹細胞の培養方法 |
| CA2857295C (en) * | 2011-12-01 | 2021-06-29 | New York Stem Cell Foundation | Automated system for producing induced pluripotent stem cells or differentiated cells |
| US10428309B2 (en) * | 2011-12-01 | 2019-10-01 | New York Stem Cell Foundation, Inc. | Systems and methods for producing stem cells and differentiated cells |
| KR20140107419A (ko) * | 2011-12-15 | 2014-09-04 | 다우 아그로사이언시즈 엘엘씨 | 애그로박테리움을 사용하는 개선된 형질전환 방법 |
| JP5274731B1 (ja) | 2011-12-22 | 2013-08-28 | 三洋電機株式会社 | 観察システム、プログラム及び観察システムの制御方法 |
| JP5991368B2 (ja) | 2012-03-16 | 2016-09-14 | 株式会社日立製作所 | 細胞シート、細胞培養方法および細胞培養装置 |
| WO2013177228A1 (en) * | 2012-05-22 | 2013-11-28 | Loma Linda University | Generation of integration/transgene-free stem cells |
| JP2015522257A (ja) | 2012-06-13 | 2015-08-06 | ステムジェント， インコーポレイテッドＳｔｅｍｇｅｎｔ， Ｉｎｃ． | 多能性幹細胞を調製する方法 |
| US9664671B2 (en) * | 2012-07-24 | 2017-05-30 | Nissan Chemical Industries, Ltd. | Culture medium composition and method of culturing cell or tissue using thereof |
| AU2013343864B2 (en) | 2012-11-09 | 2019-04-04 | BioNTech SE | Method for cellular RNA expression |
| US9738861B2 (en) | 2013-03-06 | 2017-08-22 | Kyoto University | Culture system for pluripotent stem cells and method for subculturing pluripotent stem cells |
| WO2014153115A2 (en) * | 2013-03-14 | 2014-09-25 | Children's Medical Center Corporation | Compositions and methods for reprogramming hematopoietic stem cell lineages |
| CA2895638A1 (en) * | 2013-03-15 | 2014-09-18 | Fluidigm Corporation | Methods and devices for analysis of defined multicellular combinations |
| EP2977449B1 (en) | 2013-03-21 | 2020-02-26 | Kyoto University | Pluripotent stem cell for neuronal differentiation induction |
| JP6319304B2 (ja) * | 2013-04-17 | 2018-05-09 | 日産化学工業株式会社 | 培地組成物及び当該培地組成物を用いた赤血球の製造方法 |
| US20150159127A1 (en) * | 2013-05-10 | 2015-06-11 | BioCapacity On Demand, LLC | Mobile vessel or carrier for the manufacturing production, purification and sterile fill and finish of biologics |
| US20160122720A1 (en) * | 2013-06-11 | 2016-05-05 | Kyoto University | Method of efficiently establishing induced pluripotent stem cells |
| US9580678B2 (en) * | 2013-06-21 | 2017-02-28 | The Regents Of The University Of California | Microfluidic tumor tissue dissociation device |
| US9765296B2 (en) | 2013-09-04 | 2017-09-19 | Otsuka Pharmaceutical Factory, Inc. | Method for preparing pluripotent stem cells |
| JP5751726B2 (ja) | 2013-11-12 | 2015-07-22 | 東京エレクトロン株式会社 | 多能性幹細胞の培養方法及び施設 |
| JP6668756B2 (ja) | 2014-01-23 | 2020-03-18 | 日産化学株式会社 | 培地組成物の製造方法 |
| CA2937801C (en) | 2014-01-23 | 2023-04-04 | Nissan Chemical Industries, Ltd. | Liquid culture medium composition comprising dispersed chitin or chitosan nanofibers |
| US10100286B2 (en) * | 2014-06-20 | 2018-10-16 | The Board Of Trustees Of The University Of Alabama | Systems and methods of dissociating aggregate spheres of cells |
| US20170355950A1 (en) * | 2014-12-04 | 2017-12-14 | The Arizona Board Of Regents On Behalf Of The University Of Arizona | Systems for dissociation of biological tissues |
| JP6291429B2 (ja) | 2015-01-20 | 2018-03-14 | 富士フイルム株式会社 | 細胞培養装置および細胞培養方法 |
| EP3247808B1 (en) * | 2015-01-21 | 2021-05-05 | Fred Hutchinson Cancer Research Center | Point-of-care and/or portable platform for gene therapy |
| JP6773268B2 (ja) | 2015-03-09 | 2020-10-21 | 学校法人慶應義塾 | 多能性幹細胞を所望の細胞型へ分化する方法 |
| US11286454B2 (en) | 2015-08-31 | 2022-03-29 | I Peace, Inc. | Pluripotent stem cell manufacturing system and method for producing induced pluripotent stem cells |
| KR102802007B1 (ko) * | 2016-06-08 | 2025-04-28 | 더 리전트 오브 더 유니버시티 오브 캘리포니아 | 조직 및 세포를 프로세싱하기 위한 방법 및 장치 |
-
2016
- 2016-08-30 US US15/756,029 patent/US11286454B2/en active Active
- 2016-08-30 CN CN202210349694.4A patent/CN114717183B/zh active Active
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- 2016-08-30 JP JP2018510722A patent/JP7370529B2/ja active Active
- 2016-08-30 EP EP16842835.7A patent/EP3344755A4/en active Pending
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005521405A (ja) | 2002-03-28 | 2005-07-21 | ブラスティコン ビオテクノロギッシュ フォーシュング ゲーエムベーハー | 単球を起源に持つ、脱分化したプログラム可能な幹細胞およびそれらの製造と使用 |
| WO2009096614A1 (en) | 2008-01-30 | 2009-08-06 | Mirae Biotech Co., Ltd. | Method of manufacturing induced pluripotent stem cell originated from somatic cell |
| JP2012528599A (ja) | 2009-06-05 | 2012-11-15 | セルラー ダイナミクス インターナショナル， インコーポレイテッド | リプログラミングｔ細胞および造血細胞 |
| WO2014017513A1 (ja) | 2012-07-24 | 2014-01-30 | 日産化学工業株式会社 | 培地組成物及び当該組成物を用いた細胞又は組織の培養方法 |
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