MX348271B - Metodo para la transformacion mejorada usando agrobacterium. - Google Patents

Metodo para la transformacion mejorada usando agrobacterium.

Info

Publication number
MX348271B
MX348271B MX2014007161A MX2014007161A MX348271B MX 348271 B MX348271 B MX 348271B MX 2014007161 A MX2014007161 A MX 2014007161A MX 2014007161 A MX2014007161 A MX 2014007161A MX 348271 B MX348271 B MX 348271B
Authority
MX
Mexico
Prior art keywords
agrobacterium
methods include
plants
cells
improved transformation
Prior art date
Application number
MX2014007161A
Other languages
English (en)
Other versions
MX2014007161A (es
Inventor
David Miller Paul
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of MX2014007161A publication Critical patent/MX2014007161A/es
Publication of MX348271B publication Critical patent/MX348271B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

La presente invención se refiere a métodos para aumentar la frecuencia de transformación en plantas cuando se usan Agrobacterium como el transformador. Los métodos incluyen exponer las células vegetales a células de Agrobacterium en un medio líquido que contiene un tensoactivo. Algunos métodos incluyen exponer las células vegetales a la luz continua después de la exposición a células de Agrobacterium. Ejemplos de las plantas útiles con estos métodos incluyen plantas de maíz (por ejemplo embriones de maíz inmaduros).
MX2014007161A 2011-12-15 2012-12-14 Metodo para la transformacion mejorada usando agrobacterium. MX348271B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161576138P 2011-12-15 2011-12-15
PCT/US2012/069769 WO2013090734A1 (en) 2011-12-15 2012-12-14 Method for improved transformation using agrobacterium

Publications (2)

Publication Number Publication Date
MX2014007161A MX2014007161A (es) 2014-09-22
MX348271B true MX348271B (es) 2017-06-05

Family

ID=48610504

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014007161A MX348271B (es) 2011-12-15 2012-12-14 Metodo para la transformacion mejorada usando agrobacterium.

Country Status (17)

Country Link
US (1) US20130157369A1 (es)
EP (1) EP2791340A4 (es)
JP (1) JP6170939B2 (es)
KR (1) KR20140107419A (es)
CN (1) CN104114708B (es)
AR (1) AR089254A1 (es)
AU (1) AU2012352095A1 (es)
BR (1) BR112014014405A2 (es)
CA (1) CA2858117A1 (es)
HK (1) HK1203215A1 (es)
IL (1) IL233099A0 (es)
MX (1) MX348271B (es)
PH (1) PH12014501339A1 (es)
RU (1) RU2620975C2 (es)
TW (1) TWI582233B (es)
WO (1) WO2013090734A1 (es)
ZA (1) ZA201403781B (es)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR085533A1 (es) 2011-03-23 2013-10-09 Pioneer Hi Bred Int Metodos de produccion de un locus de rasgos transgenicos complejo
CN106133138A (zh) 2013-08-22 2016-11-16 先锋国际良种公司 使用引导多核苷酸/cas内切核酸酶系统的基因组修饰及其使用方法
GB201315321D0 (en) * 2013-08-28 2013-10-09 Koninklijke Nederlandse Akademie Van Wetenschappen Transduction Buffer
KR20160065952A (ko) 2013-10-04 2016-06-09 다우 아그로사이언시즈 엘엘씨 제아 메이스 메탈로티오네인-유사 조절 요소 및 그의 용도
BR102014025574A2 (pt) 2013-10-15 2015-09-29 Dow Agrosciences Llc elementos regulatórios de zea mays e usos dos mesmos
BR102014025499A2 (pt) 2013-10-15 2015-09-29 Dow Agrosciences Llc elementos regulatórios de zea mays e uso dos mesmos
BR112017003757A2 (pt) 2014-09-12 2017-12-26 Du Pont ?plantas de milho, partes de planta de milho ou sementes de milho?
BR112017021903B1 (pt) 2015-04-15 2023-12-05 Corteva Agriscience Llc Promotor de planta para expressão de transgene
KR20170136573A (ko) 2015-04-15 2017-12-11 다우 아그로사이언시즈 엘엘씨 트랜스젠 발현을 위한 식물 프로모터
CA2996582A1 (en) * 2015-08-31 2017-03-09 I Peace, Inc. Pluripotent stem cell manufacturing system and method for producing induced pluripotent stem cells
TW201718861A (zh) 2015-09-22 2017-06-01 道禮責任有限公司 用於轉殖基因表現之植物啟動子及３’ｕｔｒ
TW201718862A (zh) 2015-09-22 2017-06-01 Dow Agrosciences Llc 用於轉殖基因表現之植物啟動子及３’ｕｔｒ
EP3365451B1 (en) 2015-10-22 2020-06-24 Dow Agrosciences LLC Plant promoter for transgene expression
US20170121726A1 (en) 2015-11-04 2017-05-04 Dow Agrosciences Llc Plant promoter for transgene expression
GB201521101D0 (en) 2015-11-30 2016-01-13 Koninklijke Nederlandse Akademie Van Wetenschappen Transduction buffer
BR112019018059A2 (pt) 2017-03-07 2020-08-04 BASF Agricultural Solutions Seed US LLC molécula de ácido nucleico recombinante, célula hospedeira, plantas, sementes transgênicas, polipeptídeo recombinante, método para produzir um polipeptídeo, método de controle de ervas daninhas, uso do ácido nucleico e produto de utilidade
BR112019018175A2 (pt) 2017-03-07 2020-04-07 BASF Agricultural Solutions Seed US LLC molécula, célula, planta, sementes, polipeptídeos recombinantes, método para produzir um polipeptídeo, planta, método para controlar ervas, uso do ácido nucleico e produto de utilidade
KR20200124702A (ko) 2018-02-23 2020-11-03 파이어니어 하이 부렛드 인터내쇼날 인코포레이팃드 신규한 cas9 오르소로그
CN112088018A (zh) * 2018-05-07 2020-12-15 先锋国际良种公司 用于在植物细胞基因组中同源定向修复双链断裂的方法和组合物
CN113166744B (zh) 2018-12-14 2025-02-07 先锋国际良种公司 用于基因组编辑的新颖crispr-cas系统
EP3898988A1 (en) * 2018-12-20 2021-10-27 Benson Hill, Inc. Pre-conditioning treatments to improve plant transformation
AR122735A1 (es) * 2020-06-24 2022-10-05 Benson Hill Inc Tratamientos de células vegetales para mejorar la transformación de la planta

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU762964B2 (en) * 1998-10-15 2003-07-10 Protein Research Trust Transformation process
BR0010069A (pt) * 1999-04-29 2002-01-22 Syngenta Ltd Polinucelotìdeo isolado, vetor, material vegetal, plantas ineiras, férteis, morfologicamente normais, plantas de milho, métodos para controlar seletivamente ervas daninhas em um campo, e para produzir vegetais que sejam substancialmente tolerantes resistentes ao herbicida glifosato, uso de polinucleotìdeo, e, métodos para selecionar material biológico transformado, e para regenerar uma planta transformada fértil para conter dna estranho
PL204789B1 (pl) * 1999-09-16 2010-02-26 Monsanto Technology Llc Promotor, rekombinowana konstrukcja DNA, transgeniczna komórka roślinna, transgeniczna roślina i sposób wytwarzania transgenicznej rośliny
WO2005076766A2 (en) * 2002-12-23 2005-08-25 Sunol Molecular Corporation Transient production of pharmaceutically important proteins in plants
CL2008001592A1 (es) * 2007-05-30 2009-03-06 Syngenta Participations Ag Celula vegetal que comprende una construccion de acido nucleico que contine una secuencia nucleotidica que codifica un citocromo p450, y metodo para conferir resistencia a un herbicida en la planta basado en la transformacion de la construccion descrita anteriormente.
EA201071114A1 (ru) * 2008-03-21 2011-04-29 Таргетед Гроут, Инк. Способ трансформации camelina, основанный на погружении цветков
CN101451149B (zh) * 2008-12-12 2011-08-10 四川农业大学 农杆菌介导的高效玉米遗传转化方法
CN104232679A (zh) * 2009-01-28 2014-12-24 巴斯夫植物科学有限公司 具有增强的产量相关性状的植物及其制备方法
CN102102109B (zh) * 2009-12-01 2014-02-12 北京未名凯拓作物设计中心有限公司 一种改进的渗透培养基浸花法转化玉米的方法
CN101775409A (zh) * 2010-03-17 2010-07-14 吉林省农业科学院 通过农杆菌浸染幼穗获得转基因玉米的方法

Also Published As

Publication number Publication date
TW201333197A (zh) 2013-08-16
EP2791340A4 (en) 2015-07-29
TWI582233B (zh) 2017-05-11
JP2015502156A (ja) 2015-01-22
IL233099A0 (en) 2014-07-31
PH12014501339A1 (en) 2014-09-15
RU2014128796A (ru) 2016-02-10
ZA201403781B (en) 2015-12-23
JP6170939B2 (ja) 2017-07-26
AR089254A1 (es) 2014-08-13
KR20140107419A (ko) 2014-09-04
CA2858117A1 (en) 2013-06-20
AU2012352095A1 (en) 2014-06-12
RU2620975C2 (ru) 2017-05-30
CN104114708A (zh) 2014-10-22
BR112014014405A2 (pt) 2017-06-13
EP2791340A1 (en) 2014-10-22
NZ625401A (en) 2017-02-24
HK1203215A1 (en) 2015-10-23
US20130157369A1 (en) 2013-06-20
WO2013090734A1 (en) 2013-06-20
MX2014007161A (es) 2014-09-22
CN104114708B (zh) 2018-04-03

Similar Documents

Publication Publication Date Title
PH12014501339A1 (en) Method for improved transformation using agrobacterium
WO2012158986A3 (en) Methods for site-specific genetic modification in stem cells using xanthomonas tal nucleases (xtn) for the creation of model organisms
PH12014501407A1 (en) Plant artificial seeds and methods for the production thereof
SG10201810830UA (en) Cell culture compositions and methods for polypeptide production
CL2015000565A1 (es) Método de integración de transgenes por ingeniería genética para producir células de planta transgénica.
AR085507A1 (es) Animales modificados geneticamente y metodos para su obtencion
TR201905503T4 (tr) Endofitik bakteriler içeren bitki tohumu üretmek için yöntem.
ZA201403678B (en) Methods and systems for growing plants using silicate-based substrates,cultivation of enhanced photosynthetic productivity and photosafening by utilization of exogenous glycopyranosides for endogenous glycopyranosyl-protein derivatives ,and formulations,processes and systems for the same
EA201491526A8 (ru) Способ культивирования бычьих и свиных сперматогониальных стволовых клеток без использования клеток-фидеров
MX2013009741A (es) Medio de cultivo celular para exposicion a uvc y metodos relacionados al mismo.
PH12014501510A1 (en) Improved pre-soaking process for biomass conversion
CR20150543A (es) Método de tratamiento de páncreas e hígado para condiciones por transplante de células madre en paredes de conducto biliar
DK2825633T3 (da) Fremgangsmåde til dyrkning af E. coli-celler til høj densitet
MX2014003005A (es) Plantas que tienen mejores rasgos relacionados con el rendimiento y un metodo para producirlas.
AR092868A1 (es) Metodo para mejorar la tolerancia al estres abiotico de plantas y plantas generadas mediante dicho metodo
WO2014012115A3 (en) Method for inducing cells to less mature state
TN2015000173A1 (en) Method to develop herbicide-resistant sugar beet plants
WO2012104731A3 (en) Methods for making cells with an extra-embryonic endodermal precursor phenotype
MX2014003372A (es) Método y sistema para la recolección de agua de una celda acuatica.
MX2016000141A (es) El uso de piridonacarboxamidas seleccionadas o sales de las mismas como sustancias activas contra el estres abiotico de las plantas.
BR112014011814A2 (pt) planta transgênica, descendência, semente, vetor e processo de produção relacionados
UY35104A (es) Medio de cultivo para celulas madre mesenquimales humanas
CL2016001674A1 (es) Casete de expresión génica, que comprende un promotor unido operativamente a un transgen, vectores y células huésped que comprenden dicho casete de expresión, así como método para la expresión de genes heterólogos en plantas.
UY36013A (es) Expresión raíz específica conferida por elementos regulatorios génicos quiméricos, cassette de expresión, vector, plantas y células transgénicas, polinucleotidos y métodos?.
WO2010092598A3 (en) Plant regeneration from immature embryos of jatropha curcas l.

Legal Events

Date Code Title Description
FG Grant or registration