DK3293256T3 - Automatiseret fremgangsmåde og apparatur til dyrkning af embryonale stamceller - Google Patents

Automatiseret fremgangsmåde og apparatur til dyrkning af embryonale stamceller

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Publication number
DK3293256T3
DK3293256T3 DK17192886.4T DK17192886T DK3293256T3 DK 3293256 T3 DK3293256 T3 DK 3293256T3 DK 17192886 T DK17192886 T DK 17192886T DK 3293256 T3 DK3293256 T3 DK 3293256T3
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Prior art keywords
stem cells
embryonic stem
automated method
culturing embryonic
culturing
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DK17192886.4T
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English (en)
Inventor
Nathaniel Beardsley
Veit Bergendahl
Megan Fitzgerald
Christine Daigh
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Fujifilm Cellular Dynamics Inc
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Publication of DK3293256T3 publication Critical patent/DK3293256T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0606Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/50Means for positioning or orientating the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C12M33/06Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C12M33/07Dosage or metering devices therefore
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48Automatic or computerized control
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70Enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999Small molecules not provided for elsewhere
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00Methods for the dissociation of cells, e.g. specific use of enzymes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Computer Hardware Design (AREA)
  • Clinical Laboratory Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Virology (AREA)
  • Transplantation (AREA)
DK17192886.4T 2007-06-29 2008-06-30 Automatiseret fremgangsmåde og apparatur til dyrkning af embryonale stamceller DK3293256T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US94701307P 2007-06-29 2007-06-29
EP08781187.3A EP2173863B1 (en) 2007-06-29 2008-06-30 Automated method and apparatus for embryonic stem cell culture

Publications (1)

Publication Number Publication Date
DK3293256T3 true DK3293256T3 (da) 2019-08-12

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
DK08781187.3T DK2173863T3 (da) 2007-06-29 2008-06-30 Automatiseret fremgangsmåde og apparatur til embryonisk stamcellekultur
DK17192886.4T DK3293256T3 (da) 2007-06-29 2008-06-30 Automatiseret fremgangsmåde og apparatur til dyrkning af embryonale stamceller

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK08781187.3T DK2173863T3 (da) 2007-06-29 2008-06-30 Automatiseret fremgangsmåde og apparatur til embryonisk stamcellekultur

Country Status (8)

Country Link
US (2) US8815585B2 (da)
EP (2) EP2173863B1 (da)
JP (4) JP5991796B2 (da)
KR (2) KR20100059789A (da)
AU (1) AU2008272949B2 (da)
CA (1) CA2691793A1 (da)
DK (2) DK2173863T3 (da)
WO (1) WO2009006422A1 (da)

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DK2173863T3 (da) 2019-01-21
KR20160005142A (ko) 2016-01-13
JP2016168056A (ja) 2016-09-23
JP2014110817A (ja) 2014-06-19
AU2008272949B2 (en) 2014-05-15
EP2173863A1 (en) 2010-04-14
JP2017023158A (ja) 2017-02-02
AU2008272949A1 (en) 2009-01-08
CA2691793A1 (en) 2009-01-08
JP2010532173A (ja) 2010-10-07
EP3293256B1 (en) 2019-05-22
WO2009006422A1 (en) 2009-01-08
JP5991796B2 (ja) 2016-09-14
US8815585B2 (en) 2014-08-26
EP3293256A1 (en) 2018-03-14
EP2173863B1 (en) 2018-10-10
US20130316445A1 (en) 2013-11-28
KR20100059789A (ko) 2010-06-04
US20090029462A1 (en) 2009-01-29

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