JP2018537955A5 - - Google Patents

Download PDF

Info

Publication number
JP2018537955A5
JP2018537955A5 JP2018517602A JP2018517602A JP2018537955A5 JP 2018537955 A5 JP2018537955 A5 JP 2018537955A5 JP 2018517602 A JP2018517602 A JP 2018517602A JP 2018517602 A JP2018517602 A JP 2018517602A JP 2018537955 A5 JP2018537955 A5 JP 2018537955A5
Authority
JP
Japan
Prior art keywords
sequence
item
target polynucleotide
primer
copies
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2018517602A
Other languages
English (en)
Japanese (ja)
Other versions
JP7008016B2 (ja
JP2018537955A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2016/056126 external-priority patent/WO2017062863A1/en
Publication of JP2018537955A publication Critical patent/JP2018537955A/ja
Publication of JP2018537955A5 publication Critical patent/JP2018537955A5/ja
Priority to JP2022001627A priority Critical patent/JP2022050563A/ja
Application granted granted Critical
Publication of JP7008016B2 publication Critical patent/JP7008016B2/ja
Priority to JP2024060374A priority patent/JP2024099542A/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2018517602A 2015-10-09 2016-10-07 増幅産生物の富化のための方法および組成物 Active JP7008016B2 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2022001627A JP2022050563A (ja) 2015-10-09 2022-01-07 増幅産生物の富化のための方法および組成物
JP2024060374A JP2024099542A (ja) 2015-10-09 2024-04-03 増幅産生物の富化のための方法および組成物

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562239690P 2015-10-09 2015-10-09
US62/239,690 2015-10-09
PCT/US2016/056126 WO2017062863A1 (en) 2015-10-09 2016-10-07 Methods and compositions for enrichment of amplification products

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2022001627A Division JP2022050563A (ja) 2015-10-09 2022-01-07 増幅産生物の富化のための方法および組成物

Publications (3)

Publication Number Publication Date
JP2018537955A JP2018537955A (ja) 2018-12-27
JP2018537955A5 true JP2018537955A5 (enExample) 2019-11-14
JP7008016B2 JP7008016B2 (ja) 2022-01-25

Family

ID=58488563

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2018517602A Active JP7008016B2 (ja) 2015-10-09 2016-10-07 増幅産生物の富化のための方法および組成物
JP2022001627A Pending JP2022050563A (ja) 2015-10-09 2022-01-07 増幅産生物の富化のための方法および組成物
JP2024060374A Pending JP2024099542A (ja) 2015-10-09 2024-04-03 増幅産生物の富化のための方法および組成物

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2022001627A Pending JP2022050563A (ja) 2015-10-09 2022-01-07 増幅産生物の富化のための方法および組成物
JP2024060374A Pending JP2024099542A (ja) 2015-10-09 2024-04-03 増幅産生物の富化のための方法および組成物

Country Status (11)

Country Link
US (3) US10752942B2 (enExample)
EP (1) EP3359693B1 (enExample)
JP (3) JP7008016B2 (enExample)
KR (1) KR20180055905A (enExample)
CN (2) CN114807323A (enExample)
AU (2) AU2016334233B2 (enExample)
HK (1) HK1259101A1 (enExample)
IL (2) IL258236B (enExample)
MX (2) MX2018004284A (enExample)
SG (1) SG11201802864RA (enExample)
WO (1) WO2017062863A1 (enExample)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
EP3080298B1 (en) 2013-12-11 2018-10-31 AccuraGen Holdings Limited Methods for detecting rare sequence variants
HK1259101A1 (zh) 2015-10-09 2019-11-22 Accuragen Holdings Limited 用於富集扩增产物的方法及组合物
EP3383994A4 (en) * 2015-12-03 2019-08-28 Accuragen Holdings Limited METHOD AND COMPOSITIONS FOR FORMING LIGATION PRODUCTS
EP3458586B1 (en) 2016-05-16 2022-12-28 Accuragen Holdings Limited Method of improved sequencing by strand identification
WO2018005811A1 (en) 2016-06-30 2018-01-04 Grail, Inc. Differential tagging of rna for preparation of a cell-free dna/rna sequencing library
JP6966052B2 (ja) 2016-08-15 2021-11-10 アキュラーゲン ホールディングス リミテッド 稀な配列変異体を検出するための組成物および方法
CN107760763B (zh) * 2017-11-20 2021-05-11 东南大学 一种用于茎环引物-滚环扩增反应的茎环引物以及茎环引物-滚环扩增的应用
CN109971825B (zh) * 2017-12-28 2020-11-10 南京金斯瑞生物科技有限公司 快速制备桑格测序模板的方法
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding
US12049665B2 (en) 2018-06-12 2024-07-30 Accuragen Holdings Limited Methods and compositions for forming ligation products
EP3650558A1 (en) * 2018-11-07 2020-05-13 Siemens Healthcare GmbH Liquid sample workflow for nanopore sequencing
CN110820051B (zh) * 2018-12-28 2023-04-28 广州表观生物科技有限公司 一种高灵敏度融合基因检测方法及其应用
KR102102107B1 (ko) * 2019-05-30 2020-04-20 단국대학교 천안캠퍼스 산학협력단 이동성 유전인자 SINE 선택적 발굴을 위한 HiSeq 시퀀서 기반의 DNA 라이브러리 제작 방법
WO2020263978A1 (en) * 2019-06-25 2020-12-30 Accuragen Holdings Limited Methods and systems for disease detection
GB201911421D0 (en) * 2019-08-09 2019-09-25 Dnae Diagnostics Ltd Methods of multiplexed isothermal amplification of nucleic acid sequences
CA3154235A1 (en) * 2019-09-13 2021-03-18 University Health Network Detection of circulating tumor dna using double stranded hybrid capture
WO2021101835A1 (en) * 2019-11-19 2021-05-27 Xiao Bing Wang Method for identifying gene fusions by circle cdna amplification
EP4077735A4 (en) * 2019-12-20 2024-01-24 AccuraGen Holdings Limited METHOD AND SYSTEMS FOR DETECTING DISEASES
KR102468839B1 (ko) 2020-06-03 2022-11-18 주식회사 제노헬릭스 Rna 검출 방법
KR20230028450A (ko) * 2020-06-26 2023-02-28 윌리엄 마쉬 라이스 유니버시티 앰플리콘 포괄적 풍부화
CN116438319A (zh) * 2020-10-21 2023-07-14 Illumina公司 包含多个插入物的测序模板以及用于提高测序通量的组合物和方法
WO2022103750A1 (en) * 2020-11-11 2022-05-19 Accuragen Holdings Limited Methods and systems for sample normalization
CN113549674B (zh) * 2021-04-22 2023-11-03 福建和瑞基因科技有限公司 一种检测样本中目标序列整合和突变的方法及其引物的设计方法和试剂盒
CN113832147B (zh) * 2021-09-08 2024-06-14 华南农业大学 一种高效的大片段dna合成与扩增的pcr引物、方法及应用

Family Cites Families (156)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US443087A (en) * 1890-12-23 Riding-saddle
US5322770A (en) 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
US5310652A (en) 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5527676A (en) 1989-03-29 1996-06-18 The Johns Hopkins University Detection of loss of the wild-type P53 gene and kits therefor
US6800617B1 (en) 1989-03-29 2004-10-05 The Johns Hopkins University Methods for restoring wild-type p53 gene function
US6677312B1 (en) 1989-03-29 2004-01-13 The Johns Hopkins University Methods for restoring wild-type p53 gene function
EP0390323B2 (en) 1989-03-29 2012-08-08 Johns Hopkins University Detection of loss of the wild-type p53 gene
US5362623A (en) 1991-06-14 1994-11-08 The John Hopkins University Sequence specific DNA binding by p53
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
EP0731175A3 (en) 1989-07-11 2004-05-26 Gen-Probe Incorporated Oligonucleotide probes for HIV nucleic acid
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5527670A (en) 1990-09-12 1996-06-18 Scientific Generics Limited Electrochemical denaturation of double-stranded nucleic acid
US5352775A (en) 1991-01-16 1994-10-04 The Johns Hopkins Univ. APC gene and nucleic acid probes derived therefrom
ES2158847T3 (es) 1991-01-16 2001-09-16 Univ Johns Hopkins Mutaciones heredadas y somaticas del gen apc en el cancer colorectal del hombre.
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5330892A (en) 1991-03-13 1994-07-19 The Johns Hopkins University MCC gene (mutated in colorectal cancer) used for diagnosis of cancer in humans
US5270184A (en) 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
US7326778B1 (en) 1993-05-05 2008-02-05 The John Hopkins University Mutator gene and hereditary non-polyposis colorectal cancer
US5492808A (en) 1993-05-05 1996-02-20 The Johns Hopkins University Means for detecting familial colon cancer (FCC)
US5591826A (en) 1993-05-05 1997-01-07 The Johns Hopkins University Human MSH2 protein
JPH08511425A (ja) 1993-06-09 1996-12-03 ガメラ バイオサイエンス コーポレイション 磁気サイクル反応
WO1995013375A1 (en) 1993-11-10 1995-05-18 The Johns Hopkins University Tumor suppressor waf1
WO1995015381A2 (en) 1993-12-02 1995-06-08 The Johns Hopkins University HUMAN MUTATOR GENE hMSH2 AND HEREDITARY NON POLYPOSIS COLORECTAL CANCER
US6380369B1 (en) 1994-01-27 2002-04-30 Human Genome Sciences, Inc. Human DNA mismatch repair proteins
EP0749496A4 (en) 1994-01-27 1999-03-03 Human Genome Sciences Inc HUMAN DNA MALFUNCTION REPAIR PROTEINS
US6482606B1 (en) 1994-01-27 2002-11-19 Human Genome Sciences, Inc. Human DNA mismatch repair polynucleotides
ATE175723T1 (de) 1994-03-15 1999-01-15 Scient Generics Ltd Elektrochemische denatusierung von doppelsträngiger nukleinsäure
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US5942391A (en) * 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US6593086B2 (en) * 1996-05-20 2003-07-15 Mount Sinai School Of Medicine Of New York University Nucleic acid amplification methods
US5705628A (en) 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5648245A (en) * 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
US5710029A (en) 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5705365A (en) 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5854033A (en) * 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
WO1997019193A2 (en) * 1995-11-21 1997-05-29 Yale University Unimolecular segment amplification and detection
US6511805B1 (en) 1996-03-15 2003-01-28 The Penn State Research Foundation Methods for detecting papillomavirus DNA in blood plasma and serum
EP0929694A4 (en) 1996-03-15 2002-05-02 Penn State Res Found DETECTION OF TUMOR-RELATED EXTRACELLULAR NUCLEIC ACID USING PLASMA OR BLOOD SERUM USING NUCLEIC ACID AMPLIFICATION TESTS
US8048629B2 (en) 1996-03-15 2011-11-01 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum
PT938320E (pt) 1996-03-26 2010-09-22 Michael S Kopreski Método que permite a utilização de arn extracelular extraído de plasma ou de soro para detectar, monitorizar ou avaliar o cancro
US5939291A (en) 1996-06-14 1999-08-17 Sarnoff Corporation Microfluidic method for nucleic acid amplification
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
EP2327797B1 (en) 1997-04-01 2015-11-25 Illumina Cambridge Limited Method of nucleic acid sequencing
GB9706654D0 (en) 1997-04-02 1997-05-21 Scient Generics Ltd Disassociation of interacting molecules
WO1998045474A1 (en) 1997-04-04 1998-10-15 Innogenetics N.V. Isothermal polymerase chain reaction by cycling the concentration of divalent metal ions
WO1999053102A1 (en) * 1998-04-16 1999-10-21 Packard Bioscience Company Analysis of polynucleotide sequence
DE1020534T1 (de) 1998-11-09 2001-03-01 Eiken Kagaku K.K., Tokio/Tokyo Verfahren zur synthetisieren von nukleinsäure
GB9903906D0 (en) 1999-02-19 1999-04-14 Microbiological Res Authority Method and apparatus for nucleic acid strand separation
CA2365123A1 (en) 1999-03-17 2000-09-21 David R. Cox Mismatch repair detection
IL145677A0 (en) * 1999-04-12 2002-06-30 Genentech Inc Pcr-based cloning method
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
WO2001018230A1 (en) 1999-09-08 2001-03-15 University Of Victoria Innovation & Development Corporation Use of psychrotrophic bacterium in biotechnology applications
US6251639B1 (en) 1999-09-13 2001-06-26 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences, using a RNA-DNA composite primer
EP1218543A2 (en) 1999-09-29 2002-07-03 Solexa Ltd. Polynucleotide sequencing
WO2001038580A2 (en) 1999-11-26 2001-05-31 Curagen Corporation Nucleic acid probe arrays
US6653079B2 (en) 2000-03-13 2003-11-25 Freshgene, Inc. Methods for detection of differences in nucleic acids
US6917726B2 (en) 2001-09-27 2005-07-12 Cornell Research Foundation, Inc. Zero-mode clad waveguides for performing spectroscopy with confined effective observation volumes
US6626906B1 (en) 2000-10-23 2003-09-30 Sdgi Holdings, Inc. Multi-planar adjustable connector
US7001724B1 (en) 2000-11-28 2006-02-21 Applera Corporation Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases
AU2002257605B2 (en) * 2001-02-27 2007-10-04 Virco Bvba Circular probe amplification (CPA) using energy-transfer primers
US6946251B2 (en) 2001-03-09 2005-09-20 Nugen Technologies, Inc. Methods and compositions for amplification of RNA sequences using RNA-DNA composite primers
US7122715B2 (en) * 2001-09-20 2006-10-17 Monsanto Technology Llc In vitro method to create circular molecules for use in transformation
US6977148B2 (en) 2001-10-15 2005-12-20 Qiagen Gmbh Multiple displacement amplification
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
US7553619B2 (en) * 2002-02-08 2009-06-30 Qiagen Gmbh Detection method using dissociated rolling circle amplification
US6815167B2 (en) * 2002-04-25 2004-11-09 Geneohm Sciences Amplification of DNA to produce single-stranded product of defined sequence and length
EP1539979B1 (en) 2002-09-20 2008-11-19 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
JP2006516410A (ja) 2003-02-03 2006-07-06 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 発現プロファイリングのためのｃＤＮＡ増幅
US20080021205A1 (en) * 2003-12-11 2008-01-24 Helen Blau Methods and Compositions for Use in Preparing Hairpin Rnas
WO2005065814A1 (en) * 2004-01-07 2005-07-21 Solexa Limited Modified molecular arrays
CN1950519A (zh) 2004-02-27 2007-04-18 哈佛大学的校长及成员们 聚合酶群落荧光原位测序珠子
US20100075384A1 (en) * 2004-03-25 2010-03-25 New England Biolabs, Inc. Helicase-dependent amplification of circular nucleic acids
US8039210B2 (en) 2004-05-14 2011-10-18 The Johns Hopkins University Protein tyrosine phosphatase mutations in cancers
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
EP2272984B1 (en) * 2005-02-01 2012-06-20 John Wayne Cancer Institute Use of ID4 for diagnosis and treatment of cancer
US7824890B2 (en) 2005-02-19 2010-11-02 Avacta Group Plc Isothermal amplification of nucleic acids
ATE406463T1 (de) 2005-04-06 2008-09-15 Maurice Stroun Methode zur krebsdiagnose mittels nachweis von dna und rna im kreislauf
US9139849B2 (en) * 2005-04-08 2015-09-22 The United States of America as Represented by the Government of the Department of Health and Human Services Rapid generation of long synthetic centromeric tandem repeats for mammalian artificial chromosome formation
US20060228721A1 (en) 2005-04-12 2006-10-12 Leamon John H Methods for determining sequence variants using ultra-deep sequencing
EP3257949A1 (en) 2005-06-15 2017-12-20 Complete Genomics Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
EP1922420B1 (en) 2005-08-19 2010-01-20 Bioventures, Inc. METHOD AND SUBSTANCES FOR ISOLATING miRNAs
CA2631248C (en) 2005-11-28 2016-01-12 Pacific Biosciences Of California, Inc. Uniform surfaces for hybrid material substrates and methods for making and using same
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
WO2007117832A2 (en) 2006-03-12 2007-10-18 Applera Corporation Methods of detecting target nucleic acids
EP2677039B8 (en) 2006-05-10 2022-10-05 DxTerity Diagnostics Incorporated Detection of nucleic acid targets using chemically reactive oligonucleotide probes
US8768629B2 (en) 2009-02-11 2014-07-01 Caris Mpi, Inc. Molecular profiling of tumors
EP2029777B1 (en) 2006-05-31 2017-03-08 Sequenom, Inc. Methods and compositions for the extraction of nucleic acid from a sample
WO2008070352A2 (en) 2006-10-27 2008-06-12 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
AU2007203390B2 (en) 2007-04-26 2012-10-04 Yew Jin Fong Improved lace fastener
FR2924440B1 (fr) * 2007-12-04 2015-01-09 Pf Medicament Nouveau procede de generation et de criblage d'une banque d'anticorps
AU2010213510B2 (en) 2009-02-16 2014-05-01 Epicentre Technologies Corporation Template-independent ligation of single-stranded DNA
WO2010117817A2 (en) 2009-03-30 2010-10-14 Life Technologies Corporation Methods for generating target specific probes for solution based capture
CN102625850B (zh) 2009-04-03 2014-11-26 蒂莫西·Z·刘 多重核酸检测方法和系统
WO2010120803A2 (en) * 2009-04-13 2010-10-21 Somagenics Inc. Methods and compositions for detection of small rnas
GB0910302D0 (en) * 2009-06-15 2009-07-29 Lumora Ltd Nucleic acid amplification
US20110237444A1 (en) 2009-11-20 2011-09-29 Life Technologies Corporation Methods of mapping genomic methylation patterns
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9315857B2 (en) * 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8889350B2 (en) 2010-03-26 2014-11-18 Swift Biosciences, Inc. Methods and compositions for isolating polynucleotides
US9096952B2 (en) * 2010-06-24 2015-08-04 Population Genetics Technologies Ltd. Methods and compositions for polynucleotide library production, immortalization and region of interest extraction
WO2012006291A2 (en) 2010-07-06 2012-01-12 Life Technologies Corporation Systems and methods to detect copy number variation
WO2012019320A1 (zh) 2010-08-11 2012-02-16 中国科学院心理研究所 一种甲基化dna的高通量测序方法及其应用
EP2426217A1 (en) 2010-09-03 2012-03-07 Centre National de la Recherche Scientifique (CNRS) Analytical methods for cell free nucleic acids and applications
CN101985654A (zh) 2010-10-21 2011-03-16 徐州医学院 检测多样本多位点snp的检测方法
PL3246416T3 (pl) 2011-04-15 2024-09-30 The Johns Hopkins University Bezpieczny system sekwencjonowania
WO2013003585A2 (en) 2011-06-29 2013-01-03 The Johns Hopkins University Enrichment of nucleic acids by complimentary capture
JP2013051956A (ja) * 2011-08-05 2013-03-21 Toshiba Corp 核酸解析法
JP5299981B1 (ja) 2011-09-08 2013-09-25 独立行政法人理化学研究所 プライマーセット及びそれを用いた標的核酸配列の増幅方法並びに変異核酸の検出方法
WO2013056178A2 (en) 2011-10-14 2013-04-18 Foundation Medicine, Inc. Novel estrogen receptor mutations and uses thereof
WO2013059740A1 (en) 2011-10-21 2013-04-25 Foundation Medicine, Inc. Novel alk and ntrk1 fusion molecules and uses thereof
WO2013074632A1 (en) 2011-11-14 2013-05-23 Rose Floyd D Mismatch nucleotide purification and identification
US20130217023A1 (en) 2012-02-22 2013-08-22 454 Life Sciences Corporation System And Method For Generation And Use Of Compact Clonally Amplified Products
DK2828218T3 (da) 2012-03-20 2020-11-02 Univ Washington Through Its Center For Commercialization Methods of lowering the error rate of massively parallel dna sequencing using duplex consensus sequencing
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
AU2013267609C1 (en) 2012-05-31 2019-01-03 Board Of Regents, The University Of Texas System Method for accurate sequencing of DNA
US10538807B2 (en) 2012-06-01 2020-01-21 Fred Hutchinson Cancer Research Center Compositions and methods for detecting rare nucleic acid molecule mutations
CA2875666A1 (en) 2012-06-14 2013-12-19 Fred Hutchinson Cancer Research Center Compositions and methods for sensitive mutation detection in nucleic acid molecules
CN107739754A (zh) 2012-07-11 2018-02-27 北京贝瑞和康生物技术有限公司 片断dna检测方法、片断dna检测试剂盒及其应用
WO2014015084A2 (en) 2012-07-17 2014-01-23 Counsyl, Inc. System and methods for detecting genetic variation
US9092401B2 (en) 2012-10-31 2015-07-28 Counsyl, Inc. System and methods for detecting genetic variation
AU2013204615A1 (en) 2012-07-20 2014-02-06 Verinata Health, Inc. Detecting and classifying copy number variation in a fetal genome
US20140066317A1 (en) 2012-09-04 2014-03-06 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
EP2914753A4 (en) 2012-10-31 2016-06-08 Becton Dickinson Co SELECTIVE AMPLIFICATION AND REAL-TIME PCR DETECTION OF RARE MUTATIONS
US10980804B2 (en) 2013-01-18 2021-04-20 Foundation Medicine, Inc. Methods of treating cholangiocarcinoma
WO2014145128A2 (en) * 2013-03-15 2014-09-18 Arnold Lyle J Methods for amplification of nucleic acids utilizing a circularized template prepared from a target nucleic acid
US9290800B2 (en) * 2013-03-15 2016-03-22 Pacific Biosciences Of California, Inc. Targeted rolling circle amplification
EP2971097B1 (en) 2013-03-15 2018-08-01 Verinata Health, Inc Generating cell-free dna libraries directly from blood
EP3795696B1 (en) 2013-03-15 2023-04-26 The Board of Trustees of the Leland Stanford Junior University Identification and use of circulating nucleic acid tumor markers
US20150033372A1 (en) * 2013-05-01 2015-01-29 Kymab Limited Human VpreB & Chimaeric Surrogate Light Chains in Transgenic Non-Human Vertebrates
US9644232B2 (en) 2013-07-26 2017-05-09 General Electric Company Method and device for collection and amplification of circulating nucleic acids
US9217167B2 (en) 2013-07-26 2015-12-22 General Electric Company Ligase-assisted nucleic acid circularization and amplification
WO2015048535A1 (en) 2013-09-27 2015-04-02 Natera, Inc. Prenatal diagnostic resting standards
EP3760733A1 (en) * 2013-10-17 2021-01-06 Takara Bio USA, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
CN105829589B (zh) 2013-11-07 2021-02-02 小利兰·斯坦福大学理事会 用于分析人体微生物组及其组分的无细胞核酸
GB201321123D0 (en) 2013-11-29 2014-01-15 Linea Ab Q Amplification of circular molecules
EP3080298B1 (en) 2013-12-11 2018-10-31 AccuraGen Holdings Limited Methods for detecting rare sequence variants
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
EP3378952B1 (en) 2013-12-28 2020-02-05 Guardant Health, Inc. Methods and systems for detecting genetic variants
CN104745679B (zh) 2013-12-31 2018-06-15 杭州贝瑞和康基因诊断技术有限公司 一种无创检测egfr基因突变的方法及试剂盒
KR102482592B1 (ko) * 2014-01-31 2022-12-30 인티그레이티드 디엔에이 테크놀로지스 아이엔씨. Dna 기질을 처리하는 개선 방법
CN106715693A (zh) * 2014-06-13 2017-05-24 伊卢米纳剑桥有限公司 用于制备序列文库的方法和组合物
EP3169793B1 (en) * 2014-07-16 2022-07-06 Tangen Biosciences Inc. Isothermal methods for amplifying nucleic acid samples
EP3201350B1 (en) 2014-09-30 2020-12-02 Global Life Sciences Solutions USA LLC Method for nucleic acid analysis directly from an unpurified biological sample
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
HK1259101A1 (zh) * 2015-10-09 2019-11-22 Accuragen Holdings Limited 用於富集扩增产物的方法及组合物
EP3383994A4 (en) 2015-12-03 2019-08-28 Accuragen Holdings Limited METHOD AND COMPOSITIONS FOR FORMING LIGATION PRODUCTS
EP3458586B1 (en) 2016-05-16 2022-12-28 Accuragen Holdings Limited Method of improved sequencing by strand identification
US10370701B2 (en) * 2016-06-17 2019-08-06 Pacific Biosciences Of California, Inc. Methods and compositions for generating asymmetrically-tagged nucleic acid fragments
US20190323073A1 (en) 2016-06-23 2019-10-24 Accuragen Holdings Limited Cell-free nucleic acid standards and uses thereof
JP6966052B2 (ja) 2016-08-15 2021-11-10 アキュラーゲン ホールディングス リミテッド 稀な配列変異体を検出するための組成物および方法
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding

Similar Documents

Publication Publication Date Title
JP2018537955A5 (enExample)
US11162094B2 (en) Capture methodologies for circulating cell free DNA
JP2017504360A5 (enExample)
JP2019531710A5 (enExample)
JP5637853B2 (ja) 縮重オリゴヌクレオチドおよびその使用
JP2017510244A5 (enExample)
JP2010519896A5 (enExample)
JP2021510541A5 (enExample)
CN103266103B (zh) 用于扩增核糖核酸的方法及分析该方法中rna或dna模板的方法
JP6310099B2 (ja) プライマーダイマーの増幅を低減する方法
JP2015526069A5 (enExample)
JP2005512577A5 (enExample)
JP2013535210A5 (enExample)
JP2021094038A5 (enExample)
JP2013507971A5 (enExample)
JP2005511096A5 (enExample)
JP2019519231A5 (enExample)
CN106661636A (zh) 衔接子连接的扩增子的制备
JP2022518917A (ja) 核酸の検出方法及びプライマーの設計方法
JP2021503903A (ja) 非対称ｐｃｒ法
CN110452966A (zh) 一种利用raa-crispr蛋白酶系统快速检测方法
US10801056B2 (en) Kit and method for detection of microRNA
EP2653559A1 (en) Polynucleotide and use thereof
WO2016174642A1 (en) Method of detecting genes responsible for resistance to beta-lactam antibiotics
CN114364813B (zh) 多重等温扩增核酸序列的方法