HK1259101A1 - 用於富集擴增產物的方法及組合物 - Google Patents

用於富集擴增產物的方法及組合物

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Publication number
HK1259101A1
HK1259101A1 HK19101601.8A HK19101601A HK1259101A1 HK 1259101 A1 HK1259101 A1 HK 1259101A1 HK 19101601 A HK19101601 A HK 19101601A HK 1259101 A1 HK1259101 A1 HK 1259101A1
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HK
Hong Kong
Prior art keywords
enrichment
compositions
methods
amplification products
amplification
Prior art date
Application number
HK19101601.8A
Other languages
English (en)
Inventor
Li Weng
Shengrong Lin
Ling Fung Tang
Original Assignee
Accuragen Holdings Ltd
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Publication date
Application filed by Accuragen Holdings Ltd filed Critical Accuragen Holdings Ltd
Publication of HK1259101A1 publication Critical patent/HK1259101A1/zh

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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • G16B25/20Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
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    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/155Modifications characterised by incorporating/generating a new priming site
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    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2525/301Hairpin oligonucleotides
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    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30Oligonucleotides characterised by their secondary structure
    • C12Q2525/307Circular oligonucleotides
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    • C12Q2531/00Reactions of nucleic acids characterised by
    • C12Q2531/10Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/125Rolling circle
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    • C12Q2537/00Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/16Assays for determining copy number or wherein the copy number is of special importance
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    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers
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    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/16Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
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  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
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  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Macromonomer-Based Addition Polymer (AREA)
  • Enzymes And Modification Thereof (AREA)
HK19101601.8A 2015-10-09 2019-01-29 用於富集擴增產物的方法及組合物 HK1259101A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562239690P 2015-10-09 2015-10-09
PCT/US2016/056126 WO2017062863A1 (en) 2015-10-09 2016-10-07 Methods and compositions for enrichment of amplification products

Publications (1)

Publication Number Publication Date
HK1259101A1 true HK1259101A1 (zh) 2019-11-22

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Country Status (12)

Country Link
US (3) US10752942B2 (zh)
EP (1) EP3359693A4 (zh)
JP (3) JP7008016B2 (zh)
KR (1) KR20180055905A (zh)
CN (2) CN114807323A (zh)
AU (2) AU2016334233B2 (zh)
CA (1) CA2999902A1 (zh)
HK (1) HK1259101A1 (zh)
IL (2) IL258236B (zh)
MX (2) MX2018004284A (zh)
SG (1) SG11201802864RA (zh)
WO (1) WO2017062863A1 (zh)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
EP3495506B1 (en) 2013-12-11 2023-07-12 AccuraGen Holdings Limited Methods for detecting rare sequence variants
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
JP7008016B2 (ja) 2015-10-09 2022-01-25 アキュラジェン ホールディングス リミテッド 増幅産生物の富化のための方法および組成物
CN108699505A (zh) * 2015-12-03 2018-10-23 安可济控股有限公司 用于形成连接产物的方法和组合物
US11427866B2 (en) 2016-05-16 2022-08-30 Accuragen Holdings Limited Method of improved sequencing by strand identification
CA3024984C (en) 2016-06-30 2021-12-07 Grail, Inc. Differential tagging of rna for preparation of a cell-free dna/rna sequencing library
JP6966052B2 (ja) 2016-08-15 2021-11-10 アキュラーゲン ホールディングス リミテッド 稀な配列変異体を検出するための組成物および方法
CN107177693B (zh) * 2017-07-19 2020-11-06 中山大学 一种多倍pcr扩增方法
CN107760763B (zh) * 2017-11-20 2021-05-11 东南大学 一种用于茎环引物-滚环扩增反应的茎环引物以及茎环引物-滚环扩增的应用
CN109971825B (zh) * 2017-12-28 2020-11-10 南京金斯瑞生物科技有限公司 快速制备桑格测序模板的方法
US11203782B2 (en) * 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding
US12049665B2 (en) 2018-06-12 2024-07-30 Accuragen Holdings Limited Methods and compositions for forming ligation products
EP3650558A1 (en) * 2018-11-07 2020-05-13 Siemens Healthcare GmbH Liquid sample workflow for nanopore sequencing
CN110820051B (zh) * 2018-12-28 2023-04-28 广州表观生物科技有限公司 一种高灵敏度融合基因检测方法及其应用
KR102102107B1 (ko) * 2019-05-30 2020-04-20 단국대학교 천안캠퍼스 산학협력단 이동성 유전인자 SINE 선택적 발굴을 위한 HiSeq 시퀀서 기반의 DNA 라이브러리 제작 방법
EP3990548A4 (en) * 2019-06-25 2023-07-26 AccuraGen Holdings Limited DISEASE DETECTION METHODS AND SYSTEMS
GB201911421D0 (en) * 2019-08-09 2019-09-25 Dnae Diagnostics Ltd Methods of multiplexed isothermal amplification of nucleic acid sequences
WO2021046655A1 (en) * 2019-09-13 2021-03-18 University Health Network Detection of circulating tumor dna using double stranded hybrid capture
EP4061963A1 (en) * 2019-11-19 2022-09-28 Xbf Llc Method for identifying gene fusions by circle cdna amplification
CN115151657A (zh) * 2019-12-20 2022-10-04 安可济控股有限公司 用于疾病检测的方法和系统
KR102468839B1 (ko) * 2020-06-03 2022-11-18 주식회사 제노헬릭스 Rna 검출 방법
EP4232600A2 (en) * 2020-10-21 2023-08-30 Illumina, Inc. Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput
WO2022103750A1 (en) * 2020-11-11 2022-05-19 Accuragen Holdings Limited Methods and systems for sample normalization
CN113549674B (zh) * 2021-04-22 2023-11-03 福建和瑞基因科技有限公司 一种检测样本中目标序列整合和突变的方法及其引物的设计方法和试剂盒
CN113832147B (zh) * 2021-09-08 2024-06-14 华南农业大学 一种高效的大片段dna合成与扩增的pcr引物、方法及应用

Family Cites Families (154)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US443087A (en) * 1890-12-23 Riding-saddle
US5322770A (en) 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
US5310652A (en) 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
EP0390323B2 (en) 1989-03-29 2012-08-08 Johns Hopkins University Detection of loss of the wild-type p53 gene
US6677312B1 (en) 1989-03-29 2004-01-13 The Johns Hopkins University Methods for restoring wild-type p53 gene function
US6800617B1 (en) 1989-03-29 2004-10-05 The Johns Hopkins University Methods for restoring wild-type p53 gene function
US5362623A (en) 1991-06-14 1994-11-08 The John Hopkins University Sequence specific DNA binding by p53
US5527676A (en) 1989-03-29 1996-06-18 The Johns Hopkins University Detection of loss of the wild-type P53 gene and kits therefor
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
ES2091225T3 (es) 1989-07-11 1996-11-01 Gen Probe Inc Metodos para la amplificacion de las secuencias de acidos nucleicos.
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5527670A (en) 1990-09-12 1996-06-18 Scientific Generics Limited Electrochemical denaturation of double-stranded nucleic acid
US5352775A (en) 1991-01-16 1994-10-04 The Johns Hopkins Univ. APC gene and nucleic acid probes derived therefrom
WO1992013103A1 (en) 1991-01-16 1992-08-06 The Johns Hopkins University Inherited and somatic mutations of apc gene in colorectal cancer of humans
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5330892A (en) 1991-03-13 1994-07-19 The Johns Hopkins University MCC gene (mutated in colorectal cancer) used for diagnosis of cancer in humans
US5270184A (en) 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
US5591826A (en) 1993-05-05 1997-01-07 The Johns Hopkins University Human MSH2 protein
US7326778B1 (en) 1993-05-05 2008-02-05 The John Hopkins University Mutator gene and hereditary non-polyposis colorectal cancer
US5492808A (en) 1993-05-05 1996-02-20 The Johns Hopkins University Means for detecting familial colon cancer (FCC)
AU690124B2 (en) 1993-06-09 1998-04-23 Gamera Bioscience Corporation Magnetic cycle reaction
WO1995013375A1 (en) 1993-11-10 1995-05-18 The Johns Hopkins University Tumor suppressor waf1
WO1995015381A2 (en) 1993-12-02 1995-06-08 The Johns Hopkins University HUMAN MUTATOR GENE hMSH2 AND HEREDITARY NON POLYPOSIS COLORECTAL CANCER
NZ281023A (en) 1994-01-27 1998-04-27 Human Genome Sciences Inc Human dna mismatch repair proteins and coding sequences
US6380369B1 (en) 1994-01-27 2002-04-30 Human Genome Sciences, Inc. Human DNA mismatch repair proteins
US6482606B1 (en) 1994-01-27 2002-11-19 Human Genome Sciences, Inc. Human DNA mismatch repair polynucleotides
WO1995025177A1 (en) 1994-03-15 1995-09-21 Scientific Generics Limited Electrochemical denaturation of double-stranded nucleic acid
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US5942391A (en) * 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US6593086B2 (en) * 1996-05-20 2003-07-15 Mount Sinai School Of Medicine Of New York University Nucleic acid amplification methods
US5705628A (en) 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5648245A (en) * 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
US5710029A (en) 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5705365A (en) 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
WO1997019193A2 (en) * 1995-11-21 1997-05-29 Yale University Unimolecular segment amplification and detection
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
CA2248981C (en) 1996-03-15 2009-11-24 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays
US8048629B2 (en) 1996-03-15 2011-11-01 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum
US6511805B1 (en) 1996-03-15 2003-01-28 The Penn State Research Foundation Methods for detecting papillomavirus DNA in blood plasma and serum
US6329179B1 (en) 1996-03-26 2001-12-11 Oncomedx, Inc. Method enabling use of extracellular RNA extracted from plasma or serum to detect, monitor or evaluate cancer
US5939291A (en) 1996-06-14 1999-08-17 Sarnoff Corporation Microfluidic method for nucleic acid amplification
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
AU6846698A (en) 1997-04-01 1998-10-22 Glaxo Group Limited Method of nucleic acid amplification
GB9706654D0 (en) 1997-04-02 1997-05-21 Scient Generics Ltd Disassociation of interacting molecules
EP0917590A1 (en) 1997-04-04 1999-05-26 Innogenetics N.V. Isothermal polymerase chain reaction by cycling the concentration of divalent metal ions
AU3567099A (en) * 1998-04-16 1999-11-01 Packard Bioscience Company Analysis of polynucleotide sequence
EP2045337B1 (en) 1998-11-09 2011-08-24 Eiken Kagaku Kabushiki Kaisha Process for synthesizing nucleic acid
GB9903906D0 (en) 1999-02-19 1999-04-14 Microbiological Res Authority Method and apparatus for nucleic acid strand separation
EP1175510B1 (en) 1999-03-17 2007-02-28 The Board Of Trustees Of The Leland Stanford Junior University Mismatch repair detection
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
AU7364700A (en) 1999-09-08 2001-04-10 University Of Victoria Innovation And Development Corporation Use of psychrotrophic bacterium in biotechnology applications
DK1218542T3 (da) 1999-09-13 2004-08-02 Nugen Technologies Inc Fremgangsmåder og sammensætninger til lineær isotermisk amplifikation af polynukleotidsekvenser
AU7537200A (en) 1999-09-29 2001-04-30 Solexa Ltd. Polynucleotide sequencing
AU783841B2 (en) 1999-11-26 2005-12-15 454 Life Sciences Corporation Nucleic acid probe arrays
US6653079B2 (en) 2000-03-13 2003-11-25 Freshgene, Inc. Methods for detection of differences in nucleic acids
US6917726B2 (en) 2001-09-27 2005-07-12 Cornell Research Foundation, Inc. Zero-mode clad waveguides for performing spectroscopy with confined effective observation volumes
US6626906B1 (en) 2000-10-23 2003-09-30 Sdgi Holdings, Inc. Multi-planar adjustable connector
US7001724B1 (en) 2000-11-28 2006-02-21 Applera Corporation Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases
ATE426041T1 (de) * 2001-02-27 2009-04-15 Virco Bvba Zirkulare sonden amplifizierung (cpa) von zirkularisierten nucleinsaure-molekulen
MXPA02012739A (es) 2001-03-09 2004-04-20 Nugen Technologies Inc Metodos y composiciones para amplificacion de secuencias de arn.
US7122715B2 (en) * 2001-09-20 2006-10-17 Monsanto Technology Llc In vitro method to create circular molecules for use in transformation
US6977148B2 (en) 2001-10-15 2005-12-20 Qiagen Gmbh Multiple displacement amplification
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
US7553619B2 (en) * 2002-02-08 2009-06-30 Qiagen Gmbh Detection method using dissociated rolling circle amplification
US6815167B2 (en) * 2002-04-25 2004-11-09 Geneohm Sciences Amplification of DNA to produce single-stranded product of defined sequence and length
DE60324810D1 (de) 2002-09-20 2009-01-02 New England Biolabs Inc HELICASE-ABHuNGIGE AMPLIFIKATION VON NUKLEINSUREN
US7309571B2 (en) * 2003-02-03 2007-12-18 Ge Healthcare Bio-Sciences Corp. Amplification of self-ligated, circularized cDNA for expression profiling
US20080021205A1 (en) * 2003-12-11 2008-01-24 Helen Blau Methods and Compositions for Use in Preparing Hairpin Rnas
US20110059865A1 (en) * 2004-01-07 2011-03-10 Mark Edward Brennan Smith Modified Molecular Arrays
CA2557841A1 (en) 2004-02-27 2005-09-09 President And Fellows Of Harvard College Polony fluorescent in situ sequencing beads
US20100075384A1 (en) * 2004-03-25 2010-03-25 New England Biolabs, Inc. Helicase-dependent amplification of circular nucleic acids
US8039210B2 (en) 2004-05-14 2011-10-18 The Johns Hopkins University Protein tyrosine phosphatase mutations in cancers
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
US7588894B2 (en) * 2005-02-01 2009-09-15 John Wayne Cancern Institute Use of ID4 for diagnosis and treatment of cancer
US7824890B2 (en) 2005-02-19 2010-11-02 Avacta Group Plc Isothermal amplification of nucleic acids
ATE406463T1 (de) 2005-04-06 2008-09-15 Maurice Stroun Methode zur krebsdiagnose mittels nachweis von dna und rna im kreislauf
US9139849B2 (en) * 2005-04-08 2015-09-22 The United States of America as Represented by the Government of the Department of Health and Human Services Rapid generation of long synthetic centromeric tandem repeats for mammalian artificial chromosome formation
US20060228721A1 (en) 2005-04-12 2006-10-12 Leamon John H Methods for determining sequence variants using ultra-deep sequencing
CA2611671C (en) 2005-06-15 2013-10-08 Callida Genomics, Inc. Single molecule arrays for genetic and chemical analysis
EP1922420B1 (en) 2005-08-19 2010-01-20 Bioventures, Inc. METHOD AND SUBSTANCES FOR ISOLATING miRNAs
CA2631248C (en) 2005-11-28 2016-01-12 Pacific Biosciences Of California, Inc. Uniform surfaces for hybrid material substrates and methods for making and using same
WO2007092538A2 (en) * 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
WO2007117832A2 (en) 2006-03-12 2007-10-18 Applera Corporation Methods of detecting target nucleic acids
US10829803B2 (en) 2006-05-10 2020-11-10 Dxterity Diagnostics Incorporated Detection of nucleic acid targets using chemically reactive oligonucleotide probes
US8768629B2 (en) 2009-02-11 2014-07-01 Caris Mpi, Inc. Molecular profiling of tumors
US8679741B2 (en) 2006-05-31 2014-03-25 Sequenom, Inc. Methods and compositions for the extraction and amplification of nucleic acid from a sample
US7910354B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
AU2007203390B2 (en) 2007-04-26 2012-10-04 Yew Jin Fong Improved lace fastener
FR2924440B1 (fr) * 2007-12-04 2015-01-09 Pf Medicament Nouveau procede de generation et de criblage d'une banque d'anticorps
CN102317475B (zh) 2009-02-16 2014-07-16 阿霹震中科技公司 单链dna的不依赖模板的连接
WO2010117817A2 (en) 2009-03-30 2010-10-14 Life Technologies Corporation Methods for generating target specific probes for solution based capture
CN102625850B (zh) 2009-04-03 2014-11-26 蒂莫西·Z·刘 多重核酸检测方法和系统
US8962253B2 (en) * 2009-04-13 2015-02-24 Somagenics Inc. Methods and compositions for detection of small RNAs
GB0910302D0 (en) * 2009-06-15 2009-07-29 Lumora Ltd Nucleic acid amplification
US20110237444A1 (en) 2009-11-20 2011-09-29 Life Technologies Corporation Methods of mapping genomic methylation patterns
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US8889350B2 (en) 2010-03-26 2014-11-18 Swift Biosciences, Inc. Methods and compositions for isolating polynucleotides
WO2011161549A2 (en) 2010-06-24 2011-12-29 Population Genetics Technologies Ltd. Methods and compositions for polynucleotide library production, immortalization and region of interest extraction
US20120046877A1 (en) 2010-07-06 2012-02-23 Life Technologies Corporation Systems and methods to detect copy number variation
CN102971434B (zh) 2010-08-11 2014-04-09 中国科学院心理研究所 一种甲基化dna的高通量测序方法及其应用
EP2426217A1 (en) 2010-09-03 2012-03-07 Centre National de la Recherche Scientifique (CNRS) Analytical methods for cell free nucleic acids and applications
CN101985654A (zh) 2010-10-21 2011-03-16 徐州医学院 检测多样本多位点snp的检测方法
US9476095B2 (en) 2011-04-15 2016-10-25 The Johns Hopkins University Safe sequencing system
US9670527B2 (en) 2011-06-29 2017-06-06 The Johns Hopkins University Enrichment of nucleic acids by complimentary capture
JP2013051956A (ja) * 2011-08-05 2013-03-21 Toshiba Corp 核酸解析法
WO2013035875A1 (ja) * 2011-09-08 2013-03-14 独立行政法人理化学研究所 プライマーセット及びそれを用いた標的核酸配列の増幅方法並びに変異核酸の検出方法
WO2013056178A2 (en) 2011-10-14 2013-04-18 Foundation Medicine, Inc. Novel estrogen receptor mutations and uses thereof
WO2013059740A1 (en) 2011-10-21 2013-04-25 Foundation Medicine, Inc. Novel alk and ntrk1 fusion molecules and uses thereof
WO2013074632A1 (en) 2011-11-14 2013-05-23 Rose Floyd D Mismatch nucleotide purification and identification
US20130217023A1 (en) 2012-02-22 2013-08-22 454 Life Sciences Corporation System And Method For Generation And Use Of Compact Clonally Amplified Products
DK2828218T3 (da) 2012-03-20 2020-11-02 Univ Washington Through Its Center For Commercialization Methods of lowering the error rate of massively parallel dna sequencing using duplex consensus sequencing
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
EP2855707B1 (en) 2012-05-31 2017-07-12 Board Of Regents, The University Of Texas System Method for accurate sequencing of dna
WO2013181276A1 (en) 2012-06-01 2013-12-05 Fred Hutchinson Cancer Research Center Compositions and methods for detecting rare nucleic acid molecule mutations
WO2013188840A1 (en) 2012-06-14 2013-12-19 Fred Hutchinson Cancer Research Center Compositions and methods for sensitive mutation detection in nucleic acid molecules
WO2014008635A1 (zh) 2012-07-11 2014-01-16 北京贝瑞和康生物技术有限公司 片断dna检测方法、片断dna检测试剂盒及其应用
CN104812947B (zh) 2012-07-17 2018-04-27 考希尔股份有限公司 检测遗传变异的系统和方法
US9092401B2 (en) 2012-10-31 2015-07-28 Counsyl, Inc. System and methods for detecting genetic variation
AU2013204615A1 (en) 2012-07-20 2014-02-06 Verinata Health, Inc. Detecting and classifying copy number variation in a fetal genome
US20140066317A1 (en) 2012-09-04 2014-03-06 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20150315636A1 (en) 2012-10-31 2015-11-05 Becton, Dickinson And Company Selective amplification and real-time pcr detection of rare mutations
US10980804B2 (en) 2013-01-18 2021-04-20 Foundation Medicine, Inc. Methods of treating cholangiocarcinoma
US9290800B2 (en) * 2013-03-15 2016-03-22 Pacific Biosciences Of California, Inc. Targeted rolling circle amplification
AU2014233373B2 (en) 2013-03-15 2019-10-24 Verinata Health, Inc. Generating cell-free DNA libraries directly from blood
EP3421613B1 (en) 2013-03-15 2020-08-19 The Board of Trustees of the Leland Stanford Junior University Identification and use of circulating nucleic acid tumor markers
US10081825B2 (en) * 2013-03-15 2018-09-25 Aegea Biotechnologies, Inc. Methods for amplification of nucleic acids utilizing a circularized template prepared from a target nucleic acid
US20150033372A1 (en) * 2013-05-01 2015-01-29 Kymab Limited Human VpreB & Chimaeric Surrogate Light Chains in Transgenic Non-Human Vertebrates
US9644232B2 (en) 2013-07-26 2017-05-09 General Electric Company Method and device for collection and amplification of circulating nucleic acids
US9217167B2 (en) 2013-07-26 2015-12-22 General Electric Company Ligase-assisted nucleic acid circularization and amplification
WO2015048535A1 (en) 2013-09-27 2015-04-02 Natera, Inc. Prenatal diagnostic resting standards
EP4389888A3 (en) * 2013-10-17 2024-09-04 Takara Bio USA, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
KR102649364B1 (ko) 2013-11-07 2024-03-20 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 인간 마이크로바이옴 및 그의 성분의 분석을 위한 무세포 핵산
GB201321123D0 (en) 2013-11-29 2014-01-15 Linea Ab Q Amplification of circular molecules
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
EP3495506B1 (en) 2013-12-11 2023-07-12 AccuraGen Holdings Limited Methods for detecting rare sequence variants
EP3087204B1 (en) 2013-12-28 2018-02-14 Guardant Health, Inc. Methods and systems for detecting genetic variants
CN104745679B (zh) 2013-12-31 2018-06-15 杭州贝瑞和康基因诊断技术有限公司 一种无创检测egfr基因突变的方法及试剂盒
CN109971826B (zh) * 2014-01-31 2022-12-30 斯威夫特生物科学股份有限公司 用于加工dna底物的改进方法
AU2015273232B2 (en) * 2014-06-13 2021-09-16 Illumina Cambridge Limited Methods and compositions for preparing sequencing libraries
CN106661600B (zh) * 2014-07-16 2021-04-06 唐恩生物科技股份有限公司 用于扩增核酸样品的等温方法
US11987836B2 (en) 2014-09-30 2024-05-21 Global Life Sciences Solutions Usa Llc Method for nucleic acid analysis directly from an unpurified biological sample
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
JP7008016B2 (ja) * 2015-10-09 2022-01-25 アキュラジェン ホールディングス リミテッド 増幅産生物の富化のための方法および組成物
CN108699505A (zh) 2015-12-03 2018-10-23 安可济控股有限公司 用于形成连接产物的方法和组合物
US11427866B2 (en) 2016-05-16 2022-08-30 Accuragen Holdings Limited Method of improved sequencing by strand identification
US10370701B2 (en) * 2016-06-17 2019-08-06 Pacific Biosciences Of California, Inc. Methods and compositions for generating asymmetrically-tagged nucleic acid fragments
US20190323073A1 (en) 2016-06-23 2019-10-24 Accuragen Holdings Limited Cell-free nucleic acid standards and uses thereof
JP6966052B2 (ja) 2016-08-15 2021-11-10 アキュラーゲン ホールディングス リミテッド 稀な配列変異体を検出するための組成物および方法
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding

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