MX2018004284A - Metodos y composiciones para el enriquecimiento de productos de amplificacion. - Google Patents
Metodos y composiciones para el enriquecimiento de productos de amplificacion.Info
- Publication number
- MX2018004284A MX2018004284A MX2018004284A MX2018004284A MX2018004284A MX 2018004284 A MX2018004284 A MX 2018004284A MX 2018004284 A MX2018004284 A MX 2018004284A MX 2018004284 A MX2018004284 A MX 2018004284A MX 2018004284 A MX2018004284 A MX 2018004284A
- Authority
- MX
- Mexico
- Prior art keywords
- methods
- amplification products
- compositions
- copies
- enrichment
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
- G16B25/20—Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/30—Oligonucleotides characterised by their secondary structure
- C12Q2525/301—Hairpin oligonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/30—Oligonucleotides characterised by their secondary structure
- C12Q2525/307—Circular oligonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/125—Rolling circle
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/16—Assays for determining copy number or wherein the copy number is of special importance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Evolutionary Biology (AREA)
- Medical Informatics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Theoretical Computer Science (AREA)
- Bioinformatics & Computational Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Macromonomer-Based Addition Polymer (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
En algunos aspectos, la presente descripción proporciona métodos para enriquecer amplicones, o productos de amplificación, que comprenden un concatémero de al menos dos o más copias de un polinucleótido objetivo. En algunas modalidades, un método comprende secuenciar los amplicones que comprenden al menos dos o más copias de un polinucleótido objetivo. En algunas modalidades, los polinucleótidos objetivo comprenden secuencias que resultan del reordenamiento cromosómico, que incluyen, pero no se limitan a, mutaciones puntuales, polimorfismos de un solo nucleótido, inserciones, deleciones y translocaciones, incluyendo genes de fusión. En algunos aspectos, la presente descripción proporciona composiciones y mezclas de reacción útiles en los métodos descritos.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201562239690P | 2015-10-09 | 2015-10-09 | |
PCT/US2016/056126 WO2017062863A1 (en) | 2015-10-09 | 2016-10-07 | Methods and compositions for enrichment of amplification products |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2018004284A true MX2018004284A (es) | 2018-05-16 |
Family
ID=58488563
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2018004284A MX2018004284A (es) | 2015-10-09 | 2016-10-07 | Metodos y composiciones para el enriquecimiento de productos de amplificacion. |
Country Status (12)
Country | Link |
---|---|
US (3) | US10752942B2 (es) |
EP (1) | EP3359693A4 (es) |
JP (2) | JP7008016B2 (es) |
KR (1) | KR20180055905A (es) |
CN (2) | CN114807323A (es) |
AU (2) | AU2016334233B2 (es) |
CA (1) | CA2999902A1 (es) |
HK (1) | HK1259101A1 (es) |
IL (2) | IL258236B (es) |
MX (1) | MX2018004284A (es) |
SG (1) | SG11201802864RA (es) |
WO (1) | WO2017062863A1 (es) |
Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11286519B2 (en) | 2013-12-11 | 2022-03-29 | Accuragen Holdings Limited | Methods and compositions for enrichment of amplification products |
CN109706222A (zh) | 2013-12-11 | 2019-05-03 | 安可济控股有限公司 | 用于检测罕见序列变体的组合物和方法 |
US11859246B2 (en) | 2013-12-11 | 2024-01-02 | Accuragen Holdings Limited | Methods and compositions for enrichment of amplification products |
EP3359693A4 (en) | 2015-10-09 | 2019-03-06 | Accuragen Holdings Limited | METHODS AND COMPOSITIONS FOR ENRICHMENT OF AMPLIFICATION PRODUCTS |
EP3383994A4 (en) * | 2015-12-03 | 2019-08-28 | Accuragen Holdings Limited | METHODS AND COMPOSITIONS FOR FORMING LIGATURE PRODUCTS |
EP3458586B1 (en) | 2016-05-16 | 2022-12-28 | Accuragen Holdings Limited | Method of improved sequencing by strand identification |
EP3831958B1 (en) | 2016-06-30 | 2023-09-06 | Grail, LLC | Differential tagging of rna for preparation of a cell-free dna/rna sequencing library |
WO2018035170A1 (en) | 2016-08-15 | 2018-02-22 | Accuragen Holdings Limited | Compositions and methods for detecting rare sequence variants |
CN107177693B (zh) * | 2017-07-19 | 2020-11-06 | 中山大学 | 一种多倍pcr扩增方法 |
CN107760763B (zh) * | 2017-11-20 | 2021-05-11 | 东南大学 | 一种用于茎环引物-滚环扩增反应的茎环引物以及茎环引物-滚环扩增的应用 |
CN109971825B (zh) * | 2017-12-28 | 2020-11-10 | 南京金斯瑞生物科技有限公司 | 快速制备桑格测序模板的方法 |
US11203782B2 (en) | 2018-03-29 | 2021-12-21 | Accuragen Holdings Limited | Compositions and methods comprising asymmetric barcoding |
EP3650558A1 (en) * | 2018-11-07 | 2020-05-13 | Siemens Healthcare GmbH | Liquid sample workflow for nanopore sequencing |
CN110820051B (zh) * | 2018-12-28 | 2023-04-28 | 广州表观生物科技有限公司 | 一种高灵敏度融合基因检测方法及其应用 |
KR102102107B1 (ko) * | 2019-05-30 | 2020-04-20 | 단국대학교 천안캠퍼스 산학협력단 | 이동성 유전인자 SINE 선택적 발굴을 위한 HiSeq 시퀀서 기반의 DNA 라이브러리 제작 방법 |
EP3990548A4 (en) * | 2019-06-25 | 2023-07-26 | AccuraGen Holdings Limited | DISEASE DETECTION METHODS AND SYSTEMS |
GB201911421D0 (en) * | 2019-08-09 | 2019-09-25 | Dnae Diagnostics Ltd | Methods of multiplexed isothermal amplification of nucleic acid sequences |
WO2021046655A1 (en) * | 2019-09-13 | 2021-03-18 | University Health Network | Detection of circulating tumor dna using double stranded hybrid capture |
EP4061963A1 (en) * | 2019-11-19 | 2022-09-28 | Xbf Llc | Method for identifying gene fusions by circle cdna amplification |
EP4077735A4 (en) * | 2019-12-20 | 2024-01-24 | Accuragen Holdings Ltd | METHOD AND SYSTEMS FOR DETECTING DISEASES |
KR102468839B1 (ko) * | 2020-06-03 | 2022-11-18 | 주식회사 제노헬릭스 | Rna 검출 방법 |
KR20230091116A (ko) * | 2020-10-21 | 2023-06-22 | 일루미나, 인코포레이티드 | 다수의 삽입체를 포함하는 시퀀싱 주형, 및 시퀀싱 처리량을 개선하기 위한 조성물 및 방법 |
WO2022103750A1 (en) * | 2020-11-11 | 2022-05-19 | Accuragen Holdings Limited | Methods and systems for sample normalization |
CN113549674B (zh) * | 2021-04-22 | 2023-11-03 | 福建和瑞基因科技有限公司 | 一种检测样本中目标序列整合和突变的方法及其引物的设计方法和试剂盒 |
Family Cites Families (154)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US443087A (en) * | 1890-12-23 | Riding-saddle | ||
US5310652A (en) | 1986-08-22 | 1994-05-10 | Hoffman-La Roche Inc. | Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription |
US5322770A (en) | 1989-12-22 | 1994-06-21 | Hoffman-Laroche Inc. | Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription |
US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
US5234809A (en) | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
EP0390323B2 (en) | 1989-03-29 | 2012-08-08 | Johns Hopkins University | Detection of loss of the wild-type p53 gene |
US6677312B1 (en) | 1989-03-29 | 2004-01-13 | The Johns Hopkins University | Methods for restoring wild-type p53 gene function |
US5362623A (en) | 1991-06-14 | 1994-11-08 | The John Hopkins University | Sequence specific DNA binding by p53 |
US5527676A (en) | 1989-03-29 | 1996-06-18 | The Johns Hopkins University | Detection of loss of the wild-type P53 gene and kits therefor |
US6800617B1 (en) | 1989-03-29 | 2004-10-05 | The Johns Hopkins University | Methods for restoring wild-type p53 gene function |
CA2020958C (en) | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
EP0731175A3 (en) | 1989-07-11 | 2004-05-26 | Gen-Probe Incorporated | Oligonucleotide probes for HIV nucleic acid |
US5494810A (en) | 1990-05-03 | 1996-02-27 | Cornell Research Foundation, Inc. | Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease |
US5527670A (en) | 1990-09-12 | 1996-06-18 | Scientific Generics Limited | Electrochemical denaturation of double-stranded nucleic acid |
US5352775A (en) | 1991-01-16 | 1994-10-04 | The Johns Hopkins Univ. | APC gene and nucleic acid probes derived therefrom |
DK0569527T3 (da) | 1991-01-16 | 2001-07-16 | Syngenta Ltd | Nedarvede og somatiske mutationer af APC-gen i colorektal cancer hos mennesker |
US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
US5330892A (en) | 1991-03-13 | 1994-07-19 | The Johns Hopkins University | MCC gene (mutated in colorectal cancer) used for diagnosis of cancer in humans |
US5270184A (en) | 1991-11-19 | 1993-12-14 | Becton, Dickinson And Company | Nucleic acid target generation |
US5591826A (en) | 1993-05-05 | 1997-01-07 | The Johns Hopkins University | Human MSH2 protein |
US7326778B1 (en) | 1993-05-05 | 2008-02-05 | The John Hopkins University | Mutator gene and hereditary non-polyposis colorectal cancer |
US5492808A (en) | 1993-05-05 | 1996-02-20 | The Johns Hopkins University | Means for detecting familial colon cancer (FCC) |
KR960703174A (ko) | 1993-06-09 | 1996-06-19 | 미안 알렉 | 자기장 순환식 반응방법(magnetic cycle reaction) |
WO1995013375A1 (en) | 1993-11-10 | 1995-05-18 | The Johns Hopkins University | Tumor suppressor waf1 |
JP4312827B2 (ja) | 1993-12-02 | 2009-08-12 | ザ・ジョーンズ・ホプキンス・ユニバーシティ | ヒトのミューテーター遺伝子hMSH2及び遺伝性非ポリープ性大腸ガン |
US6482606B1 (en) | 1994-01-27 | 2002-11-19 | Human Genome Sciences, Inc. | Human DNA mismatch repair polynucleotides |
JP3732509B2 (ja) | 1994-01-27 | 2006-01-05 | ヒューマン・ジェノム・サイエンシズ,インコーポレイテッド | ヒト・dnaミスマッチ修復蛋白 |
US6380369B1 (en) | 1994-01-27 | 2002-04-30 | Human Genome Sciences, Inc. | Human DNA mismatch repair proteins |
US6033850A (en) | 1994-03-15 | 2000-03-07 | Affymetrix, Inc. | Electrochemical denaturation of double-stranded nucleic acid |
US5648211A (en) | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
US6593086B2 (en) * | 1996-05-20 | 2003-07-15 | Mount Sinai School Of Medicine Of New York University | Nucleic acid amplification methods |
US5942391A (en) * | 1994-06-22 | 1999-08-24 | Mount Sinai School Of Medicine | Nucleic acid amplification method: ramification-extension amplification method (RAM) |
US5705628A (en) | 1994-09-20 | 1998-01-06 | Whitehead Institute For Biomedical Research | DNA purification and isolation using magnetic particles |
US5648245A (en) * | 1995-05-09 | 1997-07-15 | Carnegie Institution Of Washington | Method for constructing an oligonucleotide concatamer library by rolling circle replication |
US5705365A (en) | 1995-06-07 | 1998-01-06 | Gen-Probe Incorporated | Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
US5710029A (en) | 1995-06-07 | 1998-01-20 | Gen-Probe Incorporated | Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
WO1997019193A2 (en) * | 1995-11-21 | 1997-05-29 | Yale University | Unimolecular segment amplification and detection |
US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
CA2248981C (en) | 1996-03-15 | 2009-11-24 | The Penn State Research Foundation | Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays |
US6511805B1 (en) | 1996-03-15 | 2003-01-28 | The Penn State Research Foundation | Methods for detecting papillomavirus DNA in blood plasma and serum |
US8048629B2 (en) | 1996-03-15 | 2011-11-01 | The Penn State Research Foundation | Detection of extracellular tumor-associated nucleic acid in blood plasma or serum |
PT938320E (pt) | 1996-03-26 | 2010-09-22 | Michael S Kopreski | Método que permite a utilização de arn extracelular extraído de plasma ou de soro para detectar, monitorizar ou avaliar o cancro |
US5939291A (en) | 1996-06-14 | 1999-08-17 | Sarnoff Corporation | Microfluidic method for nucleic acid amplification |
GB9620209D0 (en) | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
ES2563643T3 (es) | 1997-04-01 | 2016-03-15 | Illumina Cambridge Limited | Método de secuenciación de ácido nucleico |
GB9706654D0 (en) | 1997-04-02 | 1997-05-21 | Scient Generics Ltd | Disassociation of interacting molecules |
CA2256563A1 (en) | 1997-04-04 | 1998-10-15 | Innogenetics N.V. | Isothermal polymerase chain reaction by cycling the concentration of divalent metal ions |
AU3567099A (en) * | 1998-04-16 | 1999-11-01 | Packard Bioscience Company | Analysis of polynucleotide sequence |
EP2287338B1 (en) | 1998-11-09 | 2012-09-05 | Eiken Kagaku Kabushiki Kaisha | Process for synthesizing nucleic acid |
GB9903906D0 (en) | 1999-02-19 | 1999-04-14 | Microbiological Res Authority | Method and apparatus for nucleic acid strand separation |
ATE355388T1 (de) | 1999-03-17 | 2006-03-15 | Univ Leland Stanford Junior | Detektion von fehlbasenpaarungen |
US20060275782A1 (en) | 1999-04-20 | 2006-12-07 | Illumina, Inc. | Detection of nucleic acid reactions on bead arrays |
US7056661B2 (en) | 1999-05-19 | 2006-06-06 | Cornell Research Foundation, Inc. | Method for sequencing nucleic acid molecules |
AU7364700A (en) | 1999-09-08 | 2001-04-10 | University Of Victoria Innovation And Development Corporation | Use of psychrotrophic bacterium in biotechnology applications |
CN102586228A (zh) | 1999-09-13 | 2012-07-18 | 纽亘技术公司 | 用于多核苷酸序列线性等温扩增的方法及组合物 |
EP1218543A2 (en) | 1999-09-29 | 2002-07-03 | Solexa Ltd. | Polynucleotide sequencing |
JP2003515149A (ja) | 1999-11-26 | 2003-04-22 | キュラジェン コーポレイション | 核酸プローブアレイ |
US6653079B2 (en) | 2000-03-13 | 2003-11-25 | Freshgene, Inc. | Methods for detection of differences in nucleic acids |
US6917726B2 (en) | 2001-09-27 | 2005-07-12 | Cornell Research Foundation, Inc. | Zero-mode clad waveguides for performing spectroscopy with confined effective observation volumes |
US6626906B1 (en) | 2000-10-23 | 2003-09-30 | Sdgi Holdings, Inc. | Multi-planar adjustable connector |
US7001724B1 (en) | 2000-11-28 | 2006-02-21 | Applera Corporation | Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases |
WO2002068683A2 (en) * | 2001-02-27 | 2002-09-06 | Virco Bvba | Circular probe amplification (cpa) using energy-transfer primers |
BR0205268A (pt) | 2001-03-09 | 2004-11-30 | Nugen Technologies Inc | Processos e composições para a mplificação de sequências de rna |
US7122715B2 (en) * | 2001-09-20 | 2006-10-17 | Monsanto Technology Llc | In vitro method to create circular molecules for use in transformation |
US6977148B2 (en) | 2001-10-15 | 2005-12-20 | Qiagen Gmbh | Multiple displacement amplification |
US20110151438A9 (en) | 2001-11-19 | 2011-06-23 | Affymetrix, Inc. | Methods of Analysis of Methylation |
US7553619B2 (en) * | 2002-02-08 | 2009-06-30 | Qiagen Gmbh | Detection method using dissociated rolling circle amplification |
US6815167B2 (en) * | 2002-04-25 | 2004-11-09 | Geneohm Sciences | Amplification of DNA to produce single-stranded product of defined sequence and length |
US7282328B2 (en) | 2002-09-20 | 2007-10-16 | New England Biolabs, Inc. | Helicase dependent amplification of nucleic acids |
WO2004070053A2 (en) | 2003-02-03 | 2004-08-19 | Amersham Biosciences Corporation | cDNA AMPLIFICATION FOR EXPRESSION PROFILING |
US20080021205A1 (en) * | 2003-12-11 | 2008-01-24 | Helen Blau | Methods and Compositions for Use in Preparing Hairpin Rnas |
JP2007525571A (ja) * | 2004-01-07 | 2007-09-06 | ソレクサ リミテッド | 修飾分子アレイ |
WO2005082098A2 (en) | 2004-02-27 | 2005-09-09 | President And Fellows Of Harvard College | Polony fluorescent in situ sequencing beads |
US20100075384A1 (en) * | 2004-03-25 | 2010-03-25 | New England Biolabs, Inc. | Helicase-dependent amplification of circular nucleic acids |
US8039210B2 (en) | 2004-05-14 | 2011-10-18 | The Johns Hopkins University | Protein tyrosine phosphatase mutations in cancers |
US7170050B2 (en) | 2004-09-17 | 2007-01-30 | Pacific Biosciences Of California, Inc. | Apparatus and methods for optical analysis of molecules |
WO2006084051A2 (en) * | 2005-02-01 | 2006-08-10 | John Wayne Cancer Institute | Use of id4 for diagnosis and treatment of cancer |
WO2006087574A2 (en) | 2005-02-19 | 2006-08-24 | Geneform Technologies Limited | Isothermal nucleic acid amplification |
ES2313143T3 (es) | 2005-04-06 | 2009-03-01 | Maurice Stroun | Metodo para el diagnostico de cancer mediante la deteccion de adn y arn circulantes. |
US9139849B2 (en) * | 2005-04-08 | 2015-09-22 | The United States of America as Represented by the Government of the Department of Health and Human Services | Rapid generation of long synthetic centromeric tandem repeats for mammalian artificial chromosome formation |
US20060228721A1 (en) | 2005-04-12 | 2006-10-12 | Leamon John H | Methods for determining sequence variants using ultra-deep sequencing |
JP5331476B2 (ja) | 2005-06-15 | 2013-10-30 | カリダ・ジェノミックス・インコーポレイテッド | 遺伝子解析および化学解析用の単分子アレイ |
EP1922420B1 (en) | 2005-08-19 | 2010-01-20 | Bioventures, Inc. | METHOD AND SUBSTANCES FOR ISOLATING miRNAs |
AU2006320739B2 (en) | 2005-11-28 | 2012-03-29 | Pacific Biosciences Of California, Inc. | Uniform surfaces for hybrid material substrates and methods for making and using same |
WO2007092538A2 (en) | 2006-02-07 | 2007-08-16 | President And Fellows Of Harvard College | Methods for making nucleotide probes for sequencing and synthesis |
WO2007117832A2 (en) | 2006-03-12 | 2007-10-18 | Applera Corporation | Methods of detecting target nucleic acids |
CA2651815A1 (en) | 2006-05-10 | 2007-11-22 | Dxterity Diagnostics | Detection of nucleic acid targets using chemically reactive oligonucleotide probes |
US8768629B2 (en) | 2009-02-11 | 2014-07-01 | Caris Mpi, Inc. | Molecular profiling of tumors |
EP2602321B1 (en) | 2006-05-31 | 2017-08-23 | Sequenom, Inc. | Methods and compositions for the extraction and amplification of nucleic acid from a sample |
US7910302B2 (en) | 2006-10-27 | 2011-03-22 | Complete Genomics, Inc. | Efficient arrays of amplified polynucleotides |
AU2007203390B2 (en) | 2007-04-26 | 2012-10-04 | Yew Jin Fong | Improved lace fastener |
FR2924440B1 (fr) * | 2007-12-04 | 2015-01-09 | Pf Medicament | Nouveau procede de generation et de criblage d'une banque d'anticorps |
AU2010213510B2 (en) | 2009-02-16 | 2014-05-01 | Epicentre Technologies Corporation | Template-independent ligation of single-stranded DNA |
WO2010117817A2 (en) | 2009-03-30 | 2010-10-14 | Life Technologies Corporation | Methods for generating target specific probes for solution based capture |
CN104404134B (zh) | 2009-04-03 | 2017-05-10 | 莱弗斯基因股份有限公司 | 多重核酸检测方法和系统 |
WO2010120803A2 (en) * | 2009-04-13 | 2010-10-21 | Somagenics Inc. | Methods and compositions for detection of small rnas |
GB0910302D0 (en) * | 2009-06-15 | 2009-07-29 | Lumora Ltd | Nucleic acid amplification |
US20110237444A1 (en) | 2009-11-20 | 2011-09-29 | Life Technologies Corporation | Methods of mapping genomic methylation patterns |
US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
US8889350B2 (en) | 2010-03-26 | 2014-11-18 | Swift Biosciences, Inc. | Methods and compositions for isolating polynucleotides |
WO2011161549A2 (en) | 2010-06-24 | 2011-12-29 | Population Genetics Technologies Ltd. | Methods and compositions for polynucleotide library production, immortalization and region of interest extraction |
US20120046877A1 (en) | 2010-07-06 | 2012-02-23 | Life Technologies Corporation | Systems and methods to detect copy number variation |
WO2012019320A1 (zh) | 2010-08-11 | 2012-02-16 | 中国科学院心理研究所 | 一种甲基化dna的高通量测序方法及其应用 |
EP2426217A1 (en) | 2010-09-03 | 2012-03-07 | Centre National de la Recherche Scientifique (CNRS) | Analytical methods for cell free nucleic acids and applications |
CN101985654A (zh) | 2010-10-21 | 2011-03-16 | 徐州医学院 | 检测多样本多位点snp的检测方法 |
EP3246416A1 (en) | 2011-04-15 | 2017-11-22 | The Johns Hopkins University | Safe sequencing system |
US9670527B2 (en) | 2011-06-29 | 2017-06-06 | The Johns Hopkins University | Enrichment of nucleic acids by complimentary capture |
CN103717752B (zh) * | 2011-08-05 | 2015-04-15 | 株式会社东芝 | 核酸分析法 |
JP5299981B1 (ja) | 2011-09-08 | 2013-09-25 | 独立行政法人理化学研究所 | プライマーセット及びそれを用いた標的核酸配列の増幅方法並びに変異核酸の検出方法 |
WO2013056178A2 (en) | 2011-10-14 | 2013-04-18 | Foundation Medicine, Inc. | Novel estrogen receptor mutations and uses thereof |
WO2013059740A1 (en) | 2011-10-21 | 2013-04-25 | Foundation Medicine, Inc. | Novel alk and ntrk1 fusion molecules and uses thereof |
WO2013074632A1 (en) | 2011-11-14 | 2013-05-23 | Rose Floyd D | Mismatch nucleotide purification and identification |
US20130217023A1 (en) | 2012-02-22 | 2013-08-22 | 454 Life Sciences Corporation | System And Method For Generation And Use Of Compact Clonally Amplified Products |
ES2828661T3 (es) | 2012-03-20 | 2021-05-27 | Univ Washington Through Its Center For Commercialization | Métodos para reducir la tasa de error de la secuenciación de ADN masiva en paralelo mediante el uso de la secuenciación de secuencia consenso bicatenaria |
US8209130B1 (en) | 2012-04-04 | 2012-06-26 | Good Start Genetics, Inc. | Sequence assembly |
WO2013181170A1 (en) | 2012-05-31 | 2013-12-05 | Board Of Regents, The University Of Texas System | Method for accurate sequencing of dna |
US10538807B2 (en) | 2012-06-01 | 2020-01-21 | Fred Hutchinson Cancer Research Center | Compositions and methods for detecting rare nucleic acid molecule mutations |
JP2015521472A (ja) | 2012-06-14 | 2015-07-30 | フレッド ハチンソン キャンサー リサーチ センター | 核酸分子における高感度変異検出のための組成物および方法 |
CN103998621A (zh) | 2012-07-11 | 2014-08-20 | 北京贝瑞和康生物技术有限公司 | 片断dna检测方法、片断dna检测试剂盒及其应用 |
US9092401B2 (en) | 2012-10-31 | 2015-07-28 | Counsyl, Inc. | System and methods for detecting genetic variation |
EP3243937A1 (en) | 2012-07-17 | 2017-11-15 | Counsyl, Inc. | System and methods for detecting genetic variation |
AU2013204615A1 (en) | 2012-07-20 | 2014-02-06 | Verinata Health, Inc. | Detecting and classifying copy number variation in a fetal genome |
US20160040229A1 (en) | 2013-08-16 | 2016-02-11 | Guardant Health, Inc. | Systems and methods to detect rare mutations and copy number variation |
US20140066317A1 (en) | 2012-09-04 | 2014-03-06 | Guardant Health, Inc. | Systems and methods to detect rare mutations and copy number variation |
EP2914753A4 (en) | 2012-10-31 | 2016-06-08 | Becton Dickinson Co | SELECTIVE AMPLIFICATION AND REAL-TIME PCR DETECTION OF RARE MUTATIONS |
CA3150658A1 (en) | 2013-01-18 | 2014-07-24 | Foundation Medicine, Inc. | Methods of treating cholangiocarcinoma |
US10017807B2 (en) | 2013-03-15 | 2018-07-10 | Verinata Health, Inc. | Generating cell-free DNA libraries directly from blood |
ES2946689T3 (es) | 2013-03-15 | 2023-07-24 | Univ Leland Stanford Junior | Identificación y uso de marcadores tumorales de ácido nucleico circulante |
US10081825B2 (en) * | 2013-03-15 | 2018-09-25 | Aegea Biotechnologies, Inc. | Methods for amplification of nucleic acids utilizing a circularized template prepared from a target nucleic acid |
US9290800B2 (en) * | 2013-03-15 | 2016-03-22 | Pacific Biosciences Of California, Inc. | Targeted rolling circle amplification |
US20150033372A1 (en) * | 2013-05-01 | 2015-01-29 | Kymab Limited | Human VpreB & Chimaeric Surrogate Light Chains in Transgenic Non-Human Vertebrates |
US9644232B2 (en) | 2013-07-26 | 2017-05-09 | General Electric Company | Method and device for collection and amplification of circulating nucleic acids |
US9217167B2 (en) | 2013-07-26 | 2015-12-22 | General Electric Company | Ligase-assisted nucleic acid circularization and amplification |
WO2015048535A1 (en) | 2013-09-27 | 2015-04-02 | Natera, Inc. | Prenatal diagnostic resting standards |
WO2015057319A1 (en) * | 2013-10-17 | 2015-04-23 | Clontech Laboratories, Inc. | Methods for adding adapters to nucleic acids and compositions for practicing the same |
IL285106B (en) | 2013-11-07 | 2022-09-01 | Univ Leland Stanford Junior | Clean nucleic acids are suitable for analyzing the human microbiome and its parts |
GB201321123D0 (en) | 2013-11-29 | 2014-01-15 | Linea Ab Q | Amplification of circular molecules |
US11859246B2 (en) | 2013-12-11 | 2024-01-02 | Accuragen Holdings Limited | Methods and compositions for enrichment of amplification products |
CN109706222A (zh) | 2013-12-11 | 2019-05-03 | 安可济控股有限公司 | 用于检测罕见序列变体的组合物和方法 |
US11286519B2 (en) | 2013-12-11 | 2022-03-29 | Accuragen Holdings Limited | Methods and compositions for enrichment of amplification products |
AU2014369841B2 (en) | 2013-12-28 | 2019-01-24 | Guardant Health, Inc. | Methods and systems for detecting genetic variants |
CN104745679B (zh) | 2013-12-31 | 2018-06-15 | 杭州贝瑞和康基因诊断技术有限公司 | 一种无创检测egfr基因突变的方法及试剂盒 |
JP6723929B2 (ja) * | 2014-01-31 | 2020-07-15 | スウィフト バイオサイエンシズ, インク.Swift Biosciences, Inc. | Dna基質を処理するための改善された方法 |
SG11201610357UA (en) * | 2014-06-13 | 2017-01-27 | Illumina Cambridge Ltd | Methods and compositions for preparing sequencing libraries |
EP3169793B1 (en) * | 2014-07-16 | 2022-07-06 | Tangen Biosciences Inc. | Isothermal methods for amplifying nucleic acid samples |
US20170298415A1 (en) | 2014-09-30 | 2017-10-19 | Ge Healthcare Bio-Sciences Corp. | Method for nucleic acid analysis directly from an unpurified biological sample |
US10844428B2 (en) | 2015-04-28 | 2020-11-24 | Illumina, Inc. | Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS) |
EP3359693A4 (en) | 2015-10-09 | 2019-03-06 | Accuragen Holdings Limited | METHODS AND COMPOSITIONS FOR ENRICHMENT OF AMPLIFICATION PRODUCTS |
EP3383994A4 (en) | 2015-12-03 | 2019-08-28 | Accuragen Holdings Limited | METHODS AND COMPOSITIONS FOR FORMING LIGATURE PRODUCTS |
EP3458586B1 (en) | 2016-05-16 | 2022-12-28 | Accuragen Holdings Limited | Method of improved sequencing by strand identification |
US10370701B2 (en) * | 2016-06-17 | 2019-08-06 | Pacific Biosciences Of California, Inc. | Methods and compositions for generating asymmetrically-tagged nucleic acid fragments |
WO2017223366A1 (en) | 2016-06-23 | 2017-12-28 | Accuragen Holdings Limited | Cell-free nucleic acid standards and uses thereof |
WO2018035170A1 (en) | 2016-08-15 | 2018-02-22 | Accuragen Holdings Limited | Compositions and methods for detecting rare sequence variants |
US11203782B2 (en) | 2018-03-29 | 2021-12-21 | Accuragen Holdings Limited | Compositions and methods comprising asymmetric barcoding |
-
2016
- 2016-10-07 EP EP16854489.8A patent/EP3359693A4/en active Pending
- 2016-10-07 CA CA2999902A patent/CA2999902A1/en active Pending
- 2016-10-07 MX MX2018004284A patent/MX2018004284A/es unknown
- 2016-10-07 CN CN202210471304.0A patent/CN114807323A/zh active Pending
- 2016-10-07 CN CN201680072543.1A patent/CN108368545B/zh active Active
- 2016-10-07 SG SG11201802864RA patent/SG11201802864RA/en unknown
- 2016-10-07 JP JP2018517602A patent/JP7008016B2/ja active Active
- 2016-10-07 AU AU2016334233A patent/AU2016334233B2/en active Active
- 2016-10-07 KR KR1020187013081A patent/KR20180055905A/ko unknown
- 2016-10-07 WO PCT/US2016/056126 patent/WO2017062863A1/en active Application Filing
-
2018
- 2018-03-20 IL IL258236A patent/IL258236B/en unknown
- 2018-04-06 US US15/947,100 patent/US10752942B2/en active Active
-
2019
- 2019-01-29 HK HK19101601.8A patent/HK1259101A1/zh unknown
-
2020
- 2020-07-13 US US16/927,898 patent/US11578359B2/en active Active
-
2021
- 2021-10-26 IL IL287586A patent/IL287586A/en unknown
-
2022
- 2022-01-07 JP JP2022001627A patent/JP2022050563A/ja active Pending
-
2023
- 2023-01-11 US US18/153,214 patent/US20230287485A1/en active Pending
- 2023-03-29 AU AU2023201912A patent/AU2023201912A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
AU2016334233A1 (en) | 2018-04-12 |
WO2017062863A1 (en) | 2017-04-13 |
US20180298434A1 (en) | 2018-10-18 |
EP3359693A1 (en) | 2018-08-15 |
EP3359693A4 (en) | 2019-03-06 |
CA2999902A1 (en) | 2017-04-13 |
US11578359B2 (en) | 2023-02-14 |
AU2016334233B2 (en) | 2023-01-05 |
HK1259101A1 (zh) | 2019-11-22 |
JP2018537955A (ja) | 2018-12-27 |
KR20180055905A (ko) | 2018-05-25 |
AU2023201912A1 (en) | 2023-05-04 |
IL258236B (en) | 2021-12-01 |
CN114807323A (zh) | 2022-07-29 |
SG11201802864RA (en) | 2018-05-30 |
US20210002716A1 (en) | 2021-01-07 |
CN108368545A (zh) | 2018-08-03 |
IL258236A (en) | 2018-05-31 |
IL287586A (en) | 2021-12-01 |
US10752942B2 (en) | 2020-08-25 |
JP2022050563A (ja) | 2022-03-30 |
CN108368545B (zh) | 2022-05-17 |
US20230287485A1 (en) | 2023-09-14 |
JP7008016B2 (ja) | 2022-01-25 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
MX2018004284A (es) | Metodos y composiciones para el enriquecimiento de productos de amplificacion. | |
BR112017012765A2 (pt) | ?métodos para editar uma sequência de nucleotídeos, célula e linhagem de e. coli e método para produzir uma célula de e. coli? | |
WO2014012107A3 (en) | Human identifiation using a panel of snps | |
NZ723399A (en) | Methods and compositions for dna profiling | |
PH12019500421A1 (en) | Distributed systems for the efficient production and use of microbe-based compositions | |
MX2017005267A (es) | Metodos y composiciones para la secuenciacion de acidos nucleicos seleccionados como diana. | |
MX2018007290A (es) | Metodos y composiciones para expresion de acido ribonucleico (arn) guia basada en polimerasa ii (pol-ii). | |
MX2017008183A (es) | Nucleosidos sustituidos, nucleotidos y analogos de estos. | |
MX2017008184A (es) | Nucleosidos sustituidos, nucleotidos y analogos de estos. | |
SG11201809961TA (en) | Variants of a dna polymerase of the polx family | |
EP3633032A3 (en) | Novel cas9 proteins and guiding features for dna targeting and genome editing | |
PT3216867T (pt) | Método para modificar a sequência de genoma para introduzir mutação específica a sequência de adn alvo por reação de remoção de bases, e complexo molecular nele utilizado | |
BR112017023189A2 (pt) | aparelho de efeito de campo e métodos para sequenciar ácidos nucleicos | |
WO2014202616A3 (en) | Rasamsonia gene and use thereof | |
MX2016009243A (es) | Métodos de mutagénesis. | |
MX2018014501A (es) | Modificacion del genoma usando sistemas guia de polinucleotido/cas endonucleasa y metodos de uso. | |
SG10201804835VA (en) | Substituted nucleosides, nucleotides and analogs thereof | |
PH12017500944A1 (en) | New 2` and/or 5` amino-acid ester phosphoramidate 3`-deoxy adenosine derivatives as anti-cancer compounds | |
NZ714415A (en) | Substituted nucleosides, nucleotides and analogs thereof | |
MX2019015188A (es) | Inserciones de adn no virales orientadas. | |
MX2022005119A (es) | Metodos de reduccion de olor. | |
MD4760B1 (ro) | Compoziţii şi metode de tratament al infecţiilor provocate de viruşii hepatitei B şi hepatitei D | |
PH12017502240A1 (en) | Recombinant microorganism for improved production of alanine | |
MY196912A (en) | Short pendant arm linkers for nucleotides in sequencing applications | |
MX2018014163A (es) | Composiciones de nucleotido marcados y metodos para el secuenciamiento de acido nucleico. |