JP2010519896A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2010519896A5 JP2010519896A5 JP2009551268A JP2009551268A JP2010519896A5 JP 2010519896 A5 JP2010519896 A5 JP 2010519896A5 JP 2009551268 A JP2009551268 A JP 2009551268A JP 2009551268 A JP2009551268 A JP 2009551268A JP 2010519896 A5 JP2010519896 A5 JP 2010519896A5
- Authority
- JP
- Japan
- Prior art keywords
- primer
- nucleic acid
- target nucleic
- nucleotide
- region
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000013615 primer Substances 0.000 claims 74
- 150000007523 nucleic acids Chemical class 0.000 claims 45
- 108020004707 nucleic acids Proteins 0.000 claims 36
- 102000039446 nucleic acids Human genes 0.000 claims 36
- 125000003729 nucleotide group Chemical group 0.000 claims 33
- 239000002773 nucleotide Substances 0.000 claims 32
- 230000000295 complement effect Effects 0.000 claims 28
- 238000000034 method Methods 0.000 claims 19
- 108091034117 Oligonucleotide Proteins 0.000 claims 18
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 9
- 230000015556 catabolic process Effects 0.000 claims 7
- 238000006731 degradation reaction Methods 0.000 claims 7
- 230000000694 effects Effects 0.000 claims 5
- 230000002452 interceptive effect Effects 0.000 claims 5
- 239000000203 mixture Substances 0.000 claims 5
- 239000002777 nucleoside Substances 0.000 claims 4
- -1 nucleoside triphosphate Chemical class 0.000 claims 4
- 235000011178 triphosphate Nutrition 0.000 claims 4
- 239000001226 triphosphate Substances 0.000 claims 4
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 3
- 230000003321 amplification Effects 0.000 claims 3
- 238000000137 annealing Methods 0.000 claims 3
- 238000009396 hybridization Methods 0.000 claims 3
- 238000003199 nucleic acid amplification method Methods 0.000 claims 3
- 108010037497 3'-nucleotidase Proteins 0.000 claims 2
- 239000003155 DNA primer Substances 0.000 claims 2
- 108060002716 Exonuclease Proteins 0.000 claims 2
- 238000005336 cracking Methods 0.000 claims 2
- 238000000354 decomposition reaction Methods 0.000 claims 2
- 102000013165 exonuclease Human genes 0.000 claims 2
- 230000037452 priming Effects 0.000 claims 2
- 108010006785 Taq Polymerase Proteins 0.000 claims 1
- JCAQMQLAHNGVPY-UUOKFMHZSA-N [(2r,3s,4r,5r)-3,4-dihydroxy-5-(2,2,4-trioxo-1h-imidazo[4,5-c][1,2,6]thiadiazin-7-yl)oxolan-2-yl]methyl dihydrogen phosphate Chemical compound O[C@@H]1[C@H](O)[C@@H](COP(O)(O)=O)O[C@H]1N1C(NS(=O)(=O)NC2=O)=C2N=C1 JCAQMQLAHNGVPY-UUOKFMHZSA-N 0.000 claims 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 230000001419 dependent effect Effects 0.000 claims 1
- 108010068698 spleen exonuclease Proteins 0.000 claims 1
- 230000002194 synthesizing effect Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0703997.7 | 2007-03-01 | ||
| GBGB0703997.7A GB0703997D0 (en) | 2007-03-01 | 2007-03-01 | Methods for detecting nucleic sequences |
| PCT/GB2008/000699 WO2008104791A1 (en) | 2007-03-01 | 2008-02-29 | Methods for detecting nucleic acid sequences |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010519896A JP2010519896A (ja) | 2010-06-10 |
| JP2010519896A5 true JP2010519896A5 (enExample) | 2011-04-21 |
| JP5235905B2 JP5235905B2 (ja) | 2013-07-10 |
Family
ID=37965756
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009551268A Expired - Fee Related JP5235905B2 (ja) | 2007-03-01 | 2008-02-29 | 核酸配列の検出方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US8440405B2 (enExample) |
| EP (1) | EP2129802B1 (enExample) |
| JP (1) | JP5235905B2 (enExample) |
| CN (1) | CN101680030B (enExample) |
| GB (1) | GB0703997D0 (enExample) |
| WO (1) | WO2008104791A1 (enExample) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0703996D0 (en) * | 2007-03-01 | 2007-04-11 | Oxitec Ltd | Nucleic acid detection |
| GB0909333D0 (en) * | 2009-06-01 | 2009-07-15 | Fu Guoliang | Multiplex amplification and detection |
| WO2010115044A2 (en) * | 2009-04-02 | 2010-10-07 | Fluidigm Corporation | Selective tagging of short nucleic acid fragments and selective protection of target sequences from degradation |
| AU2010336137B2 (en) * | 2009-12-21 | 2015-08-06 | Seegene, Inc. | TSG primer target detection |
| DE102010003781B4 (de) * | 2010-04-08 | 2012-08-16 | Aj Innuscreen Gmbh | Verfahren zum Nachweis spezifischer Nukleinsäuresequenzen |
| CN101871007B (zh) * | 2010-05-07 | 2014-04-30 | 无锡锐奇基因生物科技有限公司 | 用标记探针进行检测和熔解曲线分析的方法 |
| GB201017978D0 (en) * | 2010-10-25 | 2010-12-08 | Oxitec Ltd | Multiplex amplification and detection |
| US9255293B2 (en) * | 2010-11-01 | 2016-02-09 | Gen-Probe Incorporated | Integrated capture and amplification of target nucleic acid for sequencing |
| WO2012174289A2 (en) * | 2011-06-14 | 2012-12-20 | University Of Southern California | A novel fluorescence-based assay for the rapid detection and quantification of deoxyribonucleoside triphosphates |
| CN102242211A (zh) * | 2011-07-08 | 2011-11-16 | 无锡锐奇基因生物科技有限公司 | 检测突变核酸序列的方法及试剂盒 |
| US20140377762A1 (en) * | 2011-12-19 | 2014-12-25 | 360 Genomics Ltd. | Method for enriching and detection of variant target nucleic acids |
| CN104508492B (zh) | 2012-05-25 | 2018-07-27 | 北卡罗来纳-查佩尔山大学 | 微流体装置、用于试剂的固体支持体和相关方法 |
| EP3022323B1 (en) * | 2013-07-18 | 2019-04-24 | President and Fellows of Harvard College | Specific nucleic acid amplification with compounded selectivity |
| GB201312995D0 (en) * | 2013-07-19 | 2013-09-04 | Atlas Genetics Ltd | Methods and kits for specific nucleic acid amplification and detection |
| CN104561248A (zh) * | 2013-10-22 | 2015-04-29 | 常州金麦格生物技术有限公司 | 用于检测靶核酸的引物和其应用 |
| CN103911445B (zh) * | 2014-03-20 | 2015-08-12 | 上海科亦生物科技有限公司 | 一种as-pcr引物设计方法、基因多态性检测方法及试剂盒 |
| CN114272966B (zh) | 2015-07-22 | 2023-11-07 | 北卡罗来纳-查佩尔山大学 | 含有具有空间分离的珠保留和信号检测段的珠孔几何结构的流体装置和相关方法 |
| JP7063886B2 (ja) * | 2016-09-15 | 2022-05-09 | エフ．ホフマン－ラ ロシュ アーゲー | マルチプレックスリアルタイムｐｃｒを実施する方法 |
| CN112154190A (zh) | 2018-05-18 | 2020-12-29 | 北卡罗来纳-查佩尔山大学 | 用于改善基材的表面性质的组合物、装置和方法 |
| CN118489012A (zh) * | 2021-12-31 | 2024-08-13 | 柏锘（上海）医疗科技有限公司 | 核酸检测方法与系统 |
| CN114350774B (zh) * | 2022-01-28 | 2024-07-09 | 赛纳生物科技(北京)有限公司 | 一种芯片表面固相引物剪切效率的检测方法 |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2650840B1 (fr) | 1989-08-11 | 1991-11-29 | Bertin & Cie | Procede rapide de detection et/ou d'identification d'une seule base sur une sequence d'acide nucleique, et ses applications |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| GB9211979D0 (en) | 1992-06-05 | 1992-07-15 | Buchard Ole | Uses of nucleic acid analogues |
| US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| EP0663447B1 (en) * | 1993-12-28 | 2003-07-09 | Eiken Chemical Co., Ltd. | Method of detecting a specific polynucleotide |
| AU726501B2 (en) | 1996-06-04 | 2000-11-09 | University Of Utah Research Foundation | Monitoring hybridization during PCR |
| US6248518B1 (en) | 1996-10-29 | 2001-06-19 | Board Of Regents Of University Of Nebraska | Method for detecting point mutations in DNA utilizing fluorescence energy transfer |
| US5849497A (en) | 1997-04-03 | 1998-12-15 | The Research Foundation Of State University Of New York | Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker |
| FR2779154B1 (fr) | 1998-05-27 | 2002-07-12 | Bio Merieux | Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre |
| HK1048339A1 (zh) | 1999-03-18 | 2003-03-28 | 埃克西库恩公司 | 利用特异性lna引物检测基因突变 |
| JP2003517309A (ja) * | 1999-12-02 | 2003-05-27 | ディーエヌエー サイエンシーズ インコーポレーテッド | 単一ヌクレオチドの変異及びジェノタイピングの決定方法 |
| JP3638516B2 (ja) | 2000-09-28 | 2005-04-13 | 株式会社日立製作所 | 核酸検出方法および核酸検出キット |
| US6391592B1 (en) | 2000-12-14 | 2002-05-21 | Affymetrix, Inc. | Blocker-aided target amplification of nucleic acids |
| EP1430150B1 (en) | 2001-09-24 | 2015-08-19 | One Lambda, Inc. | Diagnostic probe detection system |
| AU2003213652A1 (en) | 2002-03-01 | 2003-09-16 | Integrated Dna Technologies, Inc. | Polynomial amplification of nucleic acids |
| US20030211483A1 (en) | 2002-05-09 | 2003-11-13 | Schroeder Benjamin G. | Methods for the enrichment of low-abundance polynucleotides |
| US20040091905A1 (en) | 2002-07-01 | 2004-05-13 | Baochuan Guo | Method for detecting mutated polynucleotides within a large population of wild-type polynucleotides |
| JP4116856B2 (ja) * | 2002-10-02 | 2008-07-09 | 富士フイルム株式会社 | １塩基多型の検出方法 |
| US20040121374A1 (en) | 2002-10-02 | 2004-06-24 | Yoshihide Iwaki | Method for detecting single nucleotide polymorphisms |
| GB2395556A (en) | 2002-11-22 | 2004-05-26 | Dynal Biotech Ltd | Nucleic acid probe |
| WO2004065628A1 (en) * | 2003-01-21 | 2004-08-05 | Guoliang Fu | Quantitative multiplex detection of nucleic acids |
| US20060183136A1 (en) | 2004-11-05 | 2006-08-17 | Genevieve Pont-Kingdon | Method for haplotyping and genotyping by melting curve analysis of hybridization probes |
| WO2007008997A2 (en) | 2005-07-12 | 2007-01-18 | The Board Of Trustees Of The Leland Stanford Junior University | Template specific inhibition of pcr |
| WO2007045890A1 (en) | 2005-10-21 | 2007-04-26 | Primerdesign Ltd | Probe |
| WO2007106534A2 (en) | 2006-03-14 | 2007-09-20 | Harbor-Ucla Research And Education Institute | Selective amplification of minority mutations using primer blocking high-affinity oligonucleotides |
-
2007
- 2007-03-01 GB GBGB0703997.7A patent/GB0703997D0/en not_active Ceased
-
2008
- 2008-02-29 EP EP08750469.2A patent/EP2129802B1/en not_active Not-in-force
- 2008-02-29 US US12/529,203 patent/US8440405B2/en not_active Expired - Fee Related
- 2008-02-29 CN CN200880012889.8A patent/CN101680030B/zh not_active Expired - Fee Related
- 2008-02-29 WO PCT/GB2008/000699 patent/WO2008104791A1/en not_active Ceased
- 2008-02-29 JP JP2009551268A patent/JP5235905B2/ja not_active Expired - Fee Related
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2010519896A5 (enExample) | ||
| CA2599647C (en) | Processes using dual specificity oligonucleotide and dual specificity oligonucleotide | |
| US20210230670A1 (en) | Solid phase nucleic acid target capture and replication using strand displacing polymerases | |
| US20170067090A1 (en) | Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides | |
| JP2017504360A5 (enExample) | ||
| JP2011502502A5 (enExample) | ||
| JP2012505641A5 (enExample) | ||
| EP1786916A4 (en) | ON PRIMER-nucleic acid amplification | |
| WO2006095981A1 (en) | Processes using dual specificity oligonucleotide and dual specificity oligonucleotide | |
| JP2007532100A5 (enExample) | ||
| JP2012520080A5 (enExample) | ||
| CN100516234C (zh) | Headloop DNA扩增 | |
| JP2005518216A (ja) | 融解温度依存ｄｎａ増幅 | |
| CN104350159B (zh) | 制备在利用核酸聚合酶来检测核酸时使用的高精度核酸的方法 | |
| JP2018516594A5 (enExample) | ||
| US10801056B2 (en) | Kit and method for detection of microRNA | |
| JP6837073B2 (ja) | プライマー伸長中のプライマー−プライマー相互作用の排除 | |
| US20070031869A1 (en) | Template specific inhibition of PCR | |
| US7501254B2 (en) | Methods and compositions for amplification and capture of nucleic acid sequences | |
| JP2009050217A (ja) | 標的ｄｎａを検出する方法 | |
| WO2009102369A4 (en) | Multiplex assay for respiratory viruses | |
| JP7306722B2 (ja) | プライマー及びその使用 | |
| KR100844010B1 (ko) | 다중 유전자 동시 증폭방법 | |
| JP2011087534A (ja) | 夾雑物の影響を排除する方法 | |
| Whitcombe | 6 Using Scorpion Primers for Genotyping |