JP2012505641A5 - - Google Patents

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Publication number
JP2012505641A5
JP2012505641A5 JP2011531404A JP2011531404A JP2012505641A5 JP 2012505641 A5 JP2012505641 A5 JP 2012505641A5 JP 2011531404 A JP2011531404 A JP 2011531404A JP 2011531404 A JP2011531404 A JP 2011531404A JP 2012505641 A5 JP2012505641 A5 JP 2012505641A5
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JP
Japan
Prior art keywords
target sequence
oligonucleotide
nucleotide
variant
complementary
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JP2011531404A
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English (en)
Japanese (ja)
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JP2012505641A (ja
JP5624044B2 (ja
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Priority claimed from PCT/EP2009/007463 external-priority patent/WO2010046067A1/en
Publication of JP2012505641A publication Critical patent/JP2012505641A/ja
Publication of JP2012505641A5 publication Critical patent/JP2012505641A5/ja
Application granted granted Critical
Publication of JP5624044B2 publication Critical patent/JP5624044B2/ja
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JP2011531404A 2008-10-20 2009-10-17 改善されたアレル−特異的増幅 Active JP5624044B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10678308P 2008-10-20 2008-10-20
US61/106,783 2008-10-20
PCT/EP2009/007463 WO2010046067A1 (en) 2008-10-20 2009-10-17 Improved allele-specific amplification

Publications (3)

Publication Number Publication Date
JP2012505641A JP2012505641A (ja) 2012-03-08
JP2012505641A5 true JP2012505641A5 (enExample) 2012-05-24
JP5624044B2 JP5624044B2 (ja) 2014-11-12

Family

ID=41557754

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2011531404A Active JP5624044B2 (ja) 2008-10-20 2009-10-17 改善されたアレル−特異的増幅

Country Status (8)

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US (2) US8586299B2 (enExample)
EP (1) EP2337865B1 (enExample)
JP (1) JP5624044B2 (enExample)
CN (2) CN105200097A (enExample)
CA (1) CA2740913C (enExample)
ES (1) ES2527653T3 (enExample)
HK (1) HK1219508A1 (enExample)
WO (1) WO2010046067A1 (enExample)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2337865B1 (en) 2008-10-20 2014-11-19 Roche Diagnostics GmbH Allele-specific amplification using a primer with a modified nucleotide
US8614071B2 (en) 2009-12-11 2013-12-24 Roche Molecular Systems, Inc. Preferential amplification of mRNA over DNA using chemically modified primers
US9238832B2 (en) * 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
US9169514B2 (en) * 2010-12-03 2015-10-27 Brandeis University Detecting nucleic acid variations within populations of genomes
US20120164641A1 (en) 2010-12-22 2012-06-28 Roche Molecular Systems, Inc. Methods and Compositions for Detecting Mutation in the Human Epidermal Growth Factor Receptor Gene
CN103797132A (zh) 2011-09-23 2014-05-14 霍夫曼-拉罗奇有限公司 G形夹用于改进的等位基因特异性pcr的用途
JP2014532407A (ja) * 2011-10-24 2014-12-08 トローバジーン インコーポレイテッド がんにおけるｂｒａｆ変異を検出する方法
US9738935B2 (en) 2011-11-10 2017-08-22 Roche Molecular Systems, Inc. Complex mutations in the epidermal growth factor receptor kinase domain
AU2012340118A1 (en) * 2011-11-17 2014-04-24 Rheonix, Inc. System and methods for selective molecular analysis
JP2015517798A (ja) * 2012-02-15 2015-06-25 ジャンセン ダイアグノスティックス，エルエルシー 低頻度変異を検出するための高感度な方法
US20140341884A1 (en) 2012-12-04 2014-11-20 Roche Molecular Systems, Inc. Novel Complex Mutations in the Epidermal Growth Factor Receptor Kinase Domain
US9382581B2 (en) 2012-12-13 2016-07-05 Roche Molecular Systems, Inc. Primers with modified phosphate and base in allele-specific PCR
WO2014140061A2 (en) * 2013-03-13 2014-09-18 Roche Diagnostics Gmbh Methods and compositions for detecting mutations in the human pi3kca (pik3ca) gene
CA2920666A1 (en) 2013-08-12 2015-02-19 Genentech, Inc. Compositions and method for treating complement-associated conditions
WO2015052147A1 (en) 2013-10-09 2015-04-16 Roche Diagnostics Gmbh Methods and compositions for detecting mutation in the human ezh2 gene
TWI769970B (zh) 2014-02-08 2022-07-11 美商建南德克公司 治療阿茲海默症之方法
CN103911445B (zh) * 2014-03-20 2015-08-12 上海科亦生物科技有限公司 一种as-pcr引物设计方法、基因多态性检测方法及试剂盒
WO2015179339A1 (en) * 2014-05-19 2015-11-26 William Marsh Rice University Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides
CN106795567B (zh) 2014-10-09 2021-07-30 豪夫迈·罗氏有限公司 在表皮生长因子受体激酶结构域中的突变
US9909169B2 (en) * 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
CN104845967B (zh) * 2015-04-15 2020-12-11 苏州新海生物科技股份有限公司 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用
CN105400900A (zh) * 2015-12-29 2016-03-16 杭州迪安生物技术有限公司 焦磷酸测序技术检测braf基因v600e微量突变试剂盒及其应用
WO2017207696A1 (en) 2016-06-01 2017-12-07 F. Hoffmann-La Roche Ag Novel mutations in anaplastic lymphoma kinase predicting response to alk inhibitor therapy in lung cancer patients
US11384376B2 (en) * 2018-05-31 2022-07-12 Roche Molecular Systems, Inc. Reagents and methods for post-synthetic modification of nucleic acids
CN110964818A (zh) * 2019-11-28 2020-04-07 重庆浦洛通基因医学研究院有限公司 一种人类braf基因v600e突变的检测试剂盒及检测方法
CN113215227A (zh) * 2021-02-04 2021-08-06 郑州华沃生物科技有限公司 一种人维生素d受体基因分型检测用的引物和探针

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DE3529478A1 (de) * 1985-08-16 1987-02-19 Boehringer Mannheim Gmbh 7-desaza-2'desoxyguanosin-nukleotide, verfahren zu deren herstellung und deren verwendung zur nukleinsaeure-sequenzierung
IE61148B1 (en) * 1988-03-10 1994-10-05 Ici Plc Method of detecting nucleotide sequences
US5521301A (en) * 1988-12-12 1996-05-28 City Of Hope Genotyping of multiple allele systems
US5639611A (en) * 1988-12-12 1997-06-17 City Of Hope Allele specific polymerase chain reaction
US5137806A (en) * 1989-12-11 1992-08-11 Board Of Regents, The University Of Texas System Methods and compositions for the detection of sequences in selected DNA molecules
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
DE69827060T2 (de) * 1997-03-20 2005-03-24 F. Hoffmann-La Roche Ag Modifizierte Primer
EP0974672B1 (en) * 1998-07-21 2003-04-09 Keygene N.V. Improved primers for AFLP amplification
US6200757B1 (en) * 1999-01-19 2001-03-13 Dade Behring Inc. Method for controlling the extension of an oligonucleotide
CN100351393C (zh) * 2001-02-15 2007-11-28 宝生物工程株式会社 核苷酸多态性的检测方法
JP2005524383A (ja) * 2001-08-28 2005-08-18 ファルマシア・アンド・アップジョン・カンパニー・エルエルシー 統合失調症の診断に用いる一塩基多型
US7408051B2 (en) * 2004-04-14 2008-08-05 Applera Corporation Modified oligonucleotides and applications thereof
EP2021488B1 (en) * 2006-04-28 2018-09-12 Igor Kutyavin Use of base-modified deoxynucleoside triphosphates to improve nucleic acid detection
EP2337865B1 (en) 2008-10-20 2014-11-19 Roche Diagnostics GmbH Allele-specific amplification using a primer with a modified nucleotide

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