CA2740913C - Improved allele-specific amplification - Google Patents
Improved allele-specific amplification Download PDFInfo
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- CA2740913C CA2740913C CA2740913A CA2740913A CA2740913C CA 2740913 C CA2740913 C CA 2740913C CA 2740913 A CA2740913 A CA 2740913A CA 2740913 A CA2740913 A CA 2740913A CA 2740913 C CA2740913 C CA 2740913C
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- oligonucleotide
- variant
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- complementary
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12Q1/6858—Allele-specific amplification
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| US9238832B2 (en) * | 2009-12-11 | 2016-01-19 | Roche Molecular Systems, Inc. | Allele-specific amplification of nucleic acids |
| US8614071B2 (en) | 2009-12-11 | 2013-12-24 | Roche Molecular Systems, Inc. | Preferential amplification of mRNA over DNA using chemically modified primers |
| EP2646576B1 (en) * | 2010-12-03 | 2015-10-28 | Brandeis University | Methods and kits for detecting nucleic acid mutants in wild-type populations |
| US20120164641A1 (en) | 2010-12-22 | 2012-06-28 | Roche Molecular Systems, Inc. | Methods and Compositions for Detecting Mutation in the Human Epidermal Growth Factor Receptor Gene |
| CA2847943C (en) * | 2011-09-23 | 2017-08-29 | F.Hoffmann-La Roche Ag | Use of g-clamp for improved allele-specific pcr |
| CA2865037A1 (en) * | 2011-10-24 | 2013-05-02 | Trovagene, Inc. | Methods of detecting braf mutations in cancer |
| US9738935B2 (en) | 2011-11-10 | 2017-08-22 | Roche Molecular Systems, Inc. | Complex mutations in the epidermal growth factor receptor kinase domain |
| JP2014533508A (ja) * | 2011-11-17 | 2014-12-15 | リーアニクス・インコーポレイテッドＲｈｅｏｎｉｘ， Ｉｎｃ． | 選択的分子分析のためのシステムおよび方法 |
| AU2013221642A1 (en) * | 2012-02-15 | 2014-09-25 | Janssen Diagnostics, Llc | Highly sensitive method for detecting low frequency mutations |
| US20140341884A1 (en) | 2012-12-04 | 2014-11-20 | Roche Molecular Systems, Inc. | Novel Complex Mutations in the Epidermal Growth Factor Receptor Kinase Domain |
| US9382581B2 (en) * | 2012-12-13 | 2016-07-05 | Roche Molecular Systems, Inc. | Primers with modified phosphate and base in allele-specific PCR |
| US20140272961A1 (en) * | 2013-03-13 | 2014-09-18 | Roche Molecular Systems, Inc. | Methods and compositions for detecting mutations in the human pi3kca (pik3ca) gene |
| SG11201601044XA (en) | 2013-08-12 | 2016-03-30 | Genentech Inc | Compositions and method for treating complement-associated conditions |
| CN105593378B (zh) | 2013-10-09 | 2019-09-20 | 豪夫迈·罗氏有限公司 | 用于在人ezh2基因中检测突变的方法和组合物 |
| AU2015213741B2 (en) | 2014-02-08 | 2020-10-08 | Genentech, Inc. | Methods of treating Alzheimer's Disease |
| CN103911445B (zh) * | 2014-03-20 | 2015-08-12 | 上海科亦生物科技有限公司 | 一种as-pcr引物设计方法、基因多态性检测方法及试剂盒 |
| EP4512908A3 (en) * | 2014-05-19 | 2025-03-26 | William Marsh Rice University | Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides |
| ES2688840T3 (es) | 2014-10-09 | 2018-11-07 | F. Hoffmann-La Roche Ag | Mutaciones en el dominio cinasa del receptor del factor de crecimiento epidérmico |
| US9909169B2 (en) * | 2014-12-17 | 2018-03-06 | Roche Molecular Systems, Inc. | Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression |
| CN104845967B (zh) * | 2015-04-15 | 2020-12-11 | 苏州新海生物科技股份有限公司 | 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用 |
| CN105400900A (zh) * | 2015-12-29 | 2016-03-16 | 杭州迪安生物技术有限公司 | 焦磷酸测序技术检测braf基因v600e微量突变试剂盒及其应用 |
| EP3464625A1 (en) | 2016-06-01 | 2019-04-10 | F. Hoffmann-La Roche AG | Novel mutations in anaplastic lymphoma kinase predicting response to alk inhibitor therapy in lung cancer patients |
| US11384376B2 (en) * | 2018-05-31 | 2022-07-12 | Roche Molecular Systems, Inc. | Reagents and methods for post-synthetic modification of nucleic acids |
| CN110964818A (zh) * | 2019-11-28 | 2020-04-07 | 重庆浦洛通基因医学研究院有限公司 | 一种人类braf基因v600e突变的检测试剂盒及检测方法 |
| CN113215227A (zh) * | 2021-02-04 | 2021-08-06 | 郑州华沃生物科技有限公司 | 一种人维生素d受体基因分型检测用的引物和探针 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3529478A1 (de) | 1985-08-16 | 1987-02-19 | Boehringer Mannheim Gmbh | 7-desaza-2'desoxyguanosin-nukleotide, verfahren zu deren herstellung und deren verwendung zur nukleinsaeure-sequenzierung |
| IE61148B1 (en) | 1988-03-10 | 1994-10-05 | Ici Plc | Method of detecting nucleotide sequences |
| US5521301A (en) | 1988-12-12 | 1996-05-28 | City Of Hope | Genotyping of multiple allele systems |
| US5639611A (en) | 1988-12-12 | 1997-06-17 | City Of Hope | Allele specific polymerase chain reaction |
| US5137806A (en) | 1989-12-11 | 1992-08-11 | Board Of Regents, The University Of Texas System | Methods and compositions for the detection of sequences in selected DNA molecules |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| EP0866071B1 (en) * | 1997-03-20 | 2004-10-20 | F. Hoffmann-La Roche Ag | Modified primers |
| DE69813203T2 (de) * | 1998-07-21 | 2004-02-12 | Keygene N.V. | Verbesserte Primer zur AFLP Amplifizierung |
| US6200757B1 (en) * | 1999-01-19 | 2001-03-13 | Dade Behring Inc. | Method for controlling the extension of an oligonucleotide |
| EA005141B1 (ru) | 2001-02-15 | 2004-12-30 | Такара Био Инк. | Способ детекции нуклеотидного полиморфизма |
| JP2005524383A (ja) * | 2001-08-28 | 2005-08-18 | ファルマシア・アンド・アップジョン・カンパニー・エルエルシー | 統合失調症の診断に用いる一塩基多型 |
| US7408051B2 (en) | 2004-04-14 | 2008-08-05 | Applera Corporation | Modified oligonucleotides and applications thereof |
| CA2650820C (en) * | 2006-04-28 | 2014-09-16 | Igor Kutyavin | Use of base-modified deoxynucleoside triphosphates to improve nucleic acid detection |
| CN105200097A (zh) | 2008-10-20 | 2015-12-30 | 霍夫曼-拉罗奇有限公司 | 改进的等位基因特异性扩增 |
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2009
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- 2009-10-17 EP EP09740853.8A patent/EP2337865B1/en active Active
- 2009-10-17 CA CA2740913A patent/CA2740913C/en active Active
- 2009-10-17 JP JP2011531404A patent/JP5624044B2/ja active Active
- 2009-10-17 WO PCT/EP2009/007463 patent/WO2010046067A1/en not_active Ceased
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- 2009-10-20 US US12/582,068 patent/US8586299B2/en active Active
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| Publication number | Publication date |
|---|---|
| EP2337865B1 (en) | 2014-11-19 |
| JP2012505641A (ja) | 2012-03-08 |
| WO2010046067A9 (en) | 2010-07-15 |
| HK1219508A1 (zh) | 2017-04-07 |
| WO2010046067A1 (en) | 2010-04-29 |
| CA2740913A1 (en) | 2010-04-29 |
| EP2337865A1 (en) | 2011-06-29 |
| US8586299B2 (en) | 2013-11-19 |
| CN105200097A (zh) | 2015-12-30 |
| CN102186996A (zh) | 2011-09-14 |
| US20100099110A1 (en) | 2010-04-22 |
| JP5624044B2 (ja) | 2014-11-12 |
| US20140242592A1 (en) | 2014-08-28 |
| US9506111B2 (en) | 2016-11-29 |
| ES2527653T3 (es) | 2015-01-28 |
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