CN105200097A - 改进的等位基因特异性扩增 - Google Patents
改进的等位基因特异性扩增 Download PDFInfo
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- CN105200097A CN105200097A CN201510625732.4A CN201510625732A CN105200097A CN 105200097 A CN105200097 A CN 105200097A CN 201510625732 A CN201510625732 A CN 201510625732A CN 105200097 A CN105200097 A CN 105200097A
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| CN110964818A (zh) * | 2019-11-28 | 2020-04-07 | 重庆浦洛通基因医学研究院有限公司 | 一种人类braf基因v600e突变的检测试剂盒及检测方法 |
| CN113215227A (zh) * | 2021-02-04 | 2021-08-06 | 郑州华沃生物科技有限公司 | 一种人维生素d受体基因分型检测用的引物和探针 |
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| EP2337865B1 (en) | 2008-10-20 | 2014-11-19 | Roche Diagnostics GmbH | Allele-specific amplification using a primer with a modified nucleotide |
| US9238832B2 (en) | 2009-12-11 | 2016-01-19 | Roche Molecular Systems, Inc. | Allele-specific amplification of nucleic acids |
| US8614071B2 (en) | 2009-12-11 | 2013-12-24 | Roche Molecular Systems, Inc. | Preferential amplification of mRNA over DNA using chemically modified primers |
| US9169514B2 (en) * | 2010-12-03 | 2015-10-27 | Brandeis University | Detecting nucleic acid variations within populations of genomes |
| US20120164641A1 (en) | 2010-12-22 | 2012-06-28 | Roche Molecular Systems, Inc. | Methods and Compositions for Detecting Mutation in the Human Epidermal Growth Factor Receptor Gene |
| EP2758546B1 (en) * | 2011-09-23 | 2017-11-22 | Roche Diagnostics GmbH | Use of g-clamp for improved allele-specific pcr |
| EP2771486A1 (en) * | 2011-10-24 | 2014-09-03 | Trovagene, Inc. | Methods of detecting braf mutations in cancer |
| US9738935B2 (en) | 2011-11-10 | 2017-08-22 | Roche Molecular Systems, Inc. | Complex mutations in the epidermal growth factor receptor kinase domain |
| AU2012340118A1 (en) * | 2011-11-17 | 2014-04-24 | Rheonix, Inc. | System and methods for selective molecular analysis |
| AU2013221642A1 (en) * | 2012-02-15 | 2014-09-25 | Janssen Diagnostics, Llc | Highly sensitive method for detecting low frequency mutations |
| US20140341884A1 (en) | 2012-12-04 | 2014-11-20 | Roche Molecular Systems, Inc. | Novel Complex Mutations in the Epidermal Growth Factor Receptor Kinase Domain |
| US9382581B2 (en) * | 2012-12-13 | 2016-07-05 | Roche Molecular Systems, Inc. | Primers with modified phosphate and base in allele-specific PCR |
| CN108130373A (zh) * | 2013-03-13 | 2018-06-08 | 豪夫迈·罗氏有限公司 | 在人pi3kca (pik3ca)基因中检测突变的方法和组合物 |
| US10093978B2 (en) | 2013-08-12 | 2018-10-09 | Genentech, Inc. | Compositions for detecting complement factor H (CFH) and complement factor I (CFI) polymorphisms |
| CN105593378B (zh) | 2013-10-09 | 2019-09-20 | 豪夫迈·罗氏有限公司 | 用于在人ezh2基因中检测突变的方法和组合物 |
| MX388168B (es) | 2014-02-08 | 2025-03-19 | Genentech Inc | Un anticuerpo anti-amiloide beta (a?) monoclonal humanizado para usarse en el tratamiento de enfermedad de alzheimer. |
| CN103911445B (zh) * | 2014-03-20 | 2015-08-12 | 上海科亦生物科技有限公司 | 一种as-pcr引物设计方法、基因多态性检测方法及试剂盒 |
| PH12016502542B1 (en) * | 2014-05-19 | 2024-01-17 | Univ Rice William M | Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides |
| US9994914B2 (en) | 2014-10-09 | 2018-06-12 | Roche Molecular Systems, Inc. | Mutation in the epidermal growth factor receptor kinase domain |
| US9909169B2 (en) | 2014-12-17 | 2018-03-06 | Roche Molecular Systems, Inc. | Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression |
| CN104845967B (zh) * | 2015-04-15 | 2020-12-11 | 苏州新海生物科技股份有限公司 | 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用 |
| CN105400900A (zh) * | 2015-12-29 | 2016-03-16 | 杭州迪安生物技术有限公司 | 焦磷酸测序技术检测braf基因v600e微量突变试剂盒及其应用 |
| JP2019519540A (ja) | 2016-06-01 | 2019-07-11 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 肺癌患者におけるａｌｋ阻害薬療法に対する応答を予測する未分化リンパ腫キナーゼにおける新規な変異 |
| US11384376B2 (en) * | 2018-05-31 | 2022-07-12 | Roche Molecular Systems, Inc. | Reagents and methods for post-synthetic modification of nucleic acids |
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| US6001611A (en) * | 1997-03-20 | 1999-12-14 | Roche Molecular Systems, Inc. | Modified nucleic acid amplification primers |
| EP0974672A1 (en) * | 1998-07-21 | 2000-01-26 | Keygene N.V. | Improved primers for AFLP amplification |
| WO2000043544A1 (en) * | 1999-01-19 | 2000-07-27 | Dade Behring Inc. | Method for controlling the extension of an oligonucleotide |
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- 2009-10-17 JP JP2011531404A patent/JP5624044B2/ja active Active
- 2009-10-17 ES ES09740853.8T patent/ES2527653T3/es active Active
- 2009-10-17 WO PCT/EP2009/007463 patent/WO2010046067A1/en not_active Ceased
- 2009-10-17 CA CA2740913A patent/CA2740913C/en active Active
- 2009-10-17 CN CN2009801413032A patent/CN102186996A/zh active Pending
- 2009-10-17 CN CN201510625732.4A patent/CN105200097A/zh active Pending
- 2009-10-20 US US12/582,068 patent/US8586299B2/en active Active
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2013
- 2013-10-11 US US14/052,416 patent/US9506111B2/en active Active
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2016
- 2016-06-28 HK HK16107476.0A patent/HK1219508A1/zh unknown
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110964818A (zh) * | 2019-11-28 | 2020-04-07 | 重庆浦洛通基因医学研究院有限公司 | 一种人类braf基因v600e突变的检测试剂盒及检测方法 |
| CN113215227A (zh) * | 2021-02-04 | 2021-08-06 | 郑州华沃生物科技有限公司 | 一种人维生素d受体基因分型检测用的引物和探针 |
Also Published As
| Publication number | Publication date |
|---|---|
| ES2527653T3 (es) | 2015-01-28 |
| CA2740913A1 (en) | 2010-04-29 |
| US20100099110A1 (en) | 2010-04-22 |
| US20140242592A1 (en) | 2014-08-28 |
| US9506111B2 (en) | 2016-11-29 |
| US8586299B2 (en) | 2013-11-19 |
| JP2012505641A (ja) | 2012-03-08 |
| EP2337865A1 (en) | 2011-06-29 |
| CN102186996A (zh) | 2011-09-14 |
| HK1219508A1 (zh) | 2017-04-07 |
| WO2010046067A9 (en) | 2010-07-15 |
| EP2337865B1 (en) | 2014-11-19 |
| CA2740913C (en) | 2015-02-10 |
| JP5624044B2 (ja) | 2014-11-12 |
| WO2010046067A1 (en) | 2010-04-29 |
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