JP2012514451A5 - - Google Patents
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- Publication number
- JP2012514451A5 JP2012514451A5 JP2011544052A JP2011544052A JP2012514451A5 JP 2012514451 A5 JP2012514451 A5 JP 2012514451A5 JP 2011544052 A JP2011544052 A JP 2011544052A JP 2011544052 A JP2011544052 A JP 2011544052A JP 2012514451 A5 JP2012514451 A5 JP 2012514451A5
- Authority
- JP
- Japan
- Prior art keywords
- oligonucleotide
- target sequence
- variants
- hybridizing
- variant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 108091034117 Oligonucleotide Proteins 0.000 claims 39
- 230000003321 amplification Effects 0.000 claims 9
- 238000003199 nucleic acid amplification method Methods 0.000 claims 9
- 150000007523 nucleic acids Chemical class 0.000 claims 9
- 108020004707 nucleic acids Proteins 0.000 claims 8
- 102000039446 nucleic acids Human genes 0.000 claims 8
- 239000011541 reaction mixture Substances 0.000 claims 6
- 238000000034 method Methods 0.000 claims 5
- 239000002773 nucleotide Substances 0.000 claims 5
- 125000003729 nucleotide group Chemical group 0.000 claims 5
- 101710163270 Nuclease Proteins 0.000 claims 3
- 125000003277 amino group Chemical group 0.000 claims 3
- 239000003153 chemical reaction reagent Substances 0.000 claims 3
- TWZSNBJODPRUAV-GZBFAFLISA-N 4-(benzylamino)-1-[(2r,4s,5r)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidin-2-one Chemical group C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)N=C(NCC=2C=CC=CC=2)C=C1 TWZSNBJODPRUAV-GZBFAFLISA-N 0.000 claims 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical group C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 2
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 238000011109 contamination Methods 0.000 claims 1
- 238000003752 polymerase chain reaction Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US14311309P | 2009-01-07 | 2009-01-07 | |
| US61/143,113 | 2009-01-07 | ||
| PCT/EP2010/000030 WO2010079127A1 (en) | 2009-01-07 | 2010-01-07 | Nucleic acid amplification with allele-specific suppression of sequence variants |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2012514451A JP2012514451A (ja) | 2012-06-28 |
| JP2012514451A5 true JP2012514451A5 (enExample) | 2012-10-25 |
| JP5785095B2 JP5785095B2 (ja) | 2015-09-24 |
Family
ID=41682254
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011544052A Active JP5785095B2 (ja) | 2009-01-07 | 2010-01-07 | 配列変種の対立遺伝子特異的抑制を用いる核酸増幅 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US8206929B2 (enExample) |
| EP (1) | EP2385991B1 (enExample) |
| JP (1) | JP5785095B2 (enExample) |
| CN (2) | CN106702000A (enExample) |
| CA (1) | CA2747068C (enExample) |
| ES (1) | ES2634428T3 (enExample) |
| WO (1) | WO2010079127A1 (enExample) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5795341B2 (ja) * | 2010-03-08 | 2015-10-14 | デイナ ファーバー キャンサー インスティチュート，インコーポレイテッド | 参照ブロック配列によるｆｕｌｌＣＯＬＤ−ＰＣＲ濃縮 |
| US11130992B2 (en) | 2011-03-31 | 2021-09-28 | Dana-Farber Cancer Institute, Inc. | Methods and compositions to enable multiplex COLD-PCR |
| WO2013010074A1 (en) | 2011-07-13 | 2013-01-17 | Primeradx, Inc. | Multimodal methods for simultaneous detection and quantification of multiple nucleic acids in a sample |
| GB201116876D0 (en) | 2011-09-30 | 2011-11-16 | Epistem Ltd | Mutational analysis of JAK2 |
| EP2770065B1 (en) | 2013-02-25 | 2017-12-13 | Seegene, Inc. | Detection of nucleotide variation on target nucleic acid sequence |
| WO2015008985A1 (en) | 2013-07-15 | 2015-01-22 | Seegene, Inc. | Detection of target nucleic acid sequence by pto cleavage and extension-dependent immobilized oligonucleotide hybridization |
| WO2015013166A1 (en) | 2013-07-24 | 2015-01-29 | Dana-Farber Cancer Institute, Inc. | Methods and compositions to enable enrichment of minor dna alleles by limiting denaturation time in pcr or simply enable enrichment of minor dna alleles by limiting denaturation time in pcr |
| EP3058105B1 (en) | 2013-10-18 | 2019-05-22 | Seegene, Inc. | Detection of target nucleic acid sequence on solid phase by pto cleavage and extension using hcto assay |
| JP2016535987A (ja) | 2013-10-20 | 2016-11-24 | トローバジーン インコーポレイテッド | 核酸配列の合成及び濃縮 |
| KR102343605B1 (ko) * | 2014-05-19 | 2021-12-27 | 윌리엄 마쉬 라이스 유니버시티 | 비대립 유전자-특이적 프라이머 및 대립 유전자-특이적 블로커 올리고뉴클레오티드를 중첩하는 조성물을 사용한 대립 유전자-특이적 증폭 |
| JP5813263B1 (ja) * | 2015-03-31 | 2015-11-17 | 日鉄住金環境株式会社 | 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド |
| US11371090B2 (en) | 2016-12-12 | 2022-06-28 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for molecular barcoding of DNA molecules prior to mutation enrichment and/or mutation detection |
| WO2019023243A1 (en) | 2017-07-24 | 2019-01-31 | Dana-Farber Cancer Institute, Inc. | METHODS AND COMPOSITIONS FOR SELECTING AND AMPLIFYING DNA TARGETS IN A SINGLE REACTION MIXTURE |
| US20250092445A1 (en) * | 2021-08-25 | 2025-03-20 | Manjing Genscript Biotech Co., Ltd. | Primer set and gene chip method for detecting single base mutation |
| JP2024531713A (ja) * | 2021-09-13 | 2024-08-29 | ネクスジェン キャンサー ディテクション エルエルシー | 試料中の標的ポリヌクレオチドを検出するための方法 |
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-
2010
- 2010-01-06 US US12/683,111 patent/US8206929B2/en active Active
- 2010-01-07 CA CA2747068A patent/CA2747068C/en active Active
- 2010-01-07 JP JP2011544052A patent/JP5785095B2/ja active Active
- 2010-01-07 ES ES10700205.7T patent/ES2634428T3/es active Active
- 2010-01-07 WO PCT/EP2010/000030 patent/WO2010079127A1/en not_active Ceased
- 2010-01-07 EP EP10700205.7A patent/EP2385991B1/en active Active
- 2010-01-07 CN CN201710109955.4A patent/CN106702000A/zh active Pending
- 2010-01-07 CN CN2010800043562A patent/CN102272333A/zh active Pending