JP5785095B2 - 配列変種の対立遺伝子特異的抑制を用いる核酸増幅 - Google Patents

配列変種の対立遺伝子特異的抑制を用いる核酸増幅 Download PDF

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JP5785095B2
JP5785095B2 JP2011544052A JP2011544052A JP5785095B2 JP 5785095 B2 JP5785095 B2 JP 5785095B2 JP 2011544052 A JP2011544052 A JP 2011544052A JP 2011544052 A JP2011544052 A JP 2011544052A JP 5785095 B2 JP5785095 B2 JP 5785095B2
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oligonucleotide
target sequence
amplification
variants
nucleic acid
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JP2012514451A (ja
JP2012514451A5 (enExample
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グロウ マイケル
グロウ マイケル
ブロフィー ビクトリア
ブロフィー ビクトリア
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
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  • Molecular Biology (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2011544052A 2009-01-07 2010-01-07 配列変種の対立遺伝子特異的抑制を用いる核酸増幅 Active JP5785095B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14311309P 2009-01-07 2009-01-07
US61/143,113 2009-01-07
PCT/EP2010/000030 WO2010079127A1 (en) 2009-01-07 2010-01-07 Nucleic acid amplification with allele-specific suppression of sequence variants

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JP2012514451A JP2012514451A (ja) 2012-06-28
JP2012514451A5 JP2012514451A5 (enExample) 2012-10-25
JP5785095B2 true JP5785095B2 (ja) 2015-09-24

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JP2011544052A Active JP5785095B2 (ja) 2009-01-07 2010-01-07 配列変種の対立遺伝子特異的抑制を用いる核酸増幅

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US (1) US8206929B2 (enExample)
EP (1) EP2385991B1 (enExample)
JP (1) JP5785095B2 (enExample)
CN (2) CN102272333A (enExample)
CA (1) CA2747068C (enExample)
ES (1) ES2634428T3 (enExample)
WO (1) WO2010079127A1 (enExample)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2545189B1 (en) * 2010-03-08 2018-01-10 Dana-Farber Cancer Institute, Inc. Full cold-pcr enrichment with reference blocking sequence
EP2691541B1 (en) 2011-03-31 2017-10-18 Dana-Farber Cancer Institute, Inc. Method for enriching in single-stranded mutant sequences from mixture of wild-type and mutant sequences
WO2013010074A1 (en) 2011-07-13 2013-01-17 Primeradx, Inc. Multimodal methods for simultaneous detection and quantification of multiple nucleic acids in a sample
GB201116876D0 (en) 2011-09-30 2011-11-16 Epistem Ltd Mutational analysis of JAK2
AU2014200958B2 (en) 2013-02-25 2016-01-14 Seegene, Inc. Detection of nucleotide variation on target nucleic acid sequence
EP3022319B1 (en) 2013-07-15 2023-05-17 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent immobilized oligonucleotide hybridization
WO2015013166A1 (en) 2013-07-24 2015-01-29 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable enrichment of minor dna alleles by limiting denaturation time in pcr or simply enable enrichment of minor dna alleles by limiting denaturation time in pcr
CN105705657B (zh) 2013-10-18 2020-07-28 Seegene株式会社 基于利用杂交-捕捉和模板化寡核苷酸的探测和标记寡核苷酸切割及延伸分析的在固相中的靶核酸序列检测
CN105283555A (zh) 2013-10-20 2016-01-27 特罗瓦基因公司 核酸序列的合成和富集
EP4512908A3 (en) * 2014-05-19 2025-03-26 William Marsh Rice University Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides
JP5813263B1 (ja) * 2015-03-31 2015-11-17 日鉄住金環境株式会社 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド
US11371090B2 (en) 2016-12-12 2022-06-28 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of DNA molecules prior to mutation enrichment and/or mutation detection
US11174511B2 (en) 2017-07-24 2021-11-16 Dana-Farber Cancer Institute, Inc. Methods and compositions for selecting and amplifying DNA targets in a single reaction mixture
US20250092445A1 (en) * 2021-08-25 2025-03-20 Manjing Genscript Biotech Co., Ltd. Primer set and gene chip method for detecting single base mutation
WO2023039228A2 (en) * 2021-09-13 2023-03-16 Nexgen Cancer Detection Llc Methods for detecting a target polynucleotide in a sample comprising a mixture of the target polynucleotide and a non-target polynucleotide

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4469863A (en) 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
US5034506A (en) 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US5235033A (en) 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
DE3529478A1 (de) 1985-08-16 1987-02-19 Boehringer Mannheim Gmbh 7-desaza-2'desoxyguanosin-nukleotide, verfahren zu deren herstellung und deren verwendung zur nukleinsaeure-sequenzierung
US5374553A (en) 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5455170A (en) 1986-08-22 1995-10-03 Hoffmann-La Roche Inc. Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
US5466591A (en) 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5405774A (en) 1986-08-22 1995-04-11 Hoffmann-La Roche Inc. DNA encoding a mutated thermostable nucleic acid polymerase enzyme from thermus species sps17
US5795762A (en) 1986-08-22 1998-08-18 Roche Molecular Systems, Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5216141A (en) 1988-06-06 1993-06-01 Benner Steven A Oligonucleotide analogs containing sulfur linkages
US5386023A (en) 1990-07-27 1995-01-31 Isis Pharmaceuticals Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling
US5602240A (en) 1990-07-27 1997-02-11 Ciba Geigy Ag. Backbone modified oligonucleotide analogs
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
EP0550696B1 (en) 1990-09-28 2000-11-22 F. Hoffmann-La Roche Ag Purified thermostable nucleic acid polymerase enzyme from thermosipho africanus
JPH04234422A (ja) 1990-10-31 1992-08-24 Internatl Business Mach Corp <Ibm> 二重硬化エポキシバックシール処方物
ATE226093T1 (de) 1991-11-26 2002-11-15 Isis Pharmaceuticals Inc Gesteigerte bildung von triple- und doppelhelices aus oligomeren mit modifizierten pyrimidinen
TW393513B (en) 1991-11-26 2000-06-11 Isis Pharmaceuticals Inc Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines
US5484908A (en) 1991-11-26 1996-01-16 Gilead Sciences, Inc. Oligonucleotides containing 5-propynyl pyrimidines
US5644048A (en) 1992-01-10 1997-07-01 Isis Pharmaceuticals, Inc. Process for preparing phosphorothioate oligonucleotides
DE4234086A1 (de) 1992-02-05 1993-08-12 Diagen Inst Molekularbio Verfahren zur bestimmung von in vitro amplifizierten nukleinsaeuresequenzen
US5637684A (en) 1994-02-23 1997-06-10 Isis Pharmaceuticals, Inc. Phosphoramidate and phosphorothioamidate oligomeric compounds
GB9513271D0 (en) 1995-06-29 1995-09-06 Johnson James G Stress free fish harvester
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
CA2591550C (en) 1996-06-04 2009-04-28 University Of Utah Research Foundation Monitoring hybridization during pcr
DE69827060T2 (de) * 1997-03-20 2005-03-24 F. Hoffmann-La Roche Ag Modifizierte Primer
US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
US6228628B1 (en) 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
JP3274396B2 (ja) 1997-11-07 2002-04-15 株式会社東芝 パターン測定方法
CN100432237C (zh) * 1999-03-19 2008-11-12 宝生物工程株式会社 用于扩增核酸序列的方法
ES2269113T3 (es) 1999-03-24 2007-04-01 Exiqon A/S Sintesis mejorada de -2.2.1 / biciclo-nucleosidos.
DE60014399T2 (de) 1999-05-04 2005-12-08 Ortho-Clinical Diagnostics, Inc. Schnelle und wirksame dns-immobilisierung aus einer probe ohne die verwendung von zelllysereagenzien
CN1254548C (zh) * 2000-08-23 2006-05-03 宝生物工程株式会社 核酸扩增方法
US6479242B1 (en) * 2000-10-26 2002-11-12 Cleveland State University Method for genotyping of single nucleotide polymorphism
GB0101397D0 (en) * 2001-01-19 2001-03-07 Amersham Pharm Biotech Uk Ltd Suppression of non-specific nucleic acid amplication
EP1253205A1 (en) 2001-04-24 2002-10-30 LION Bioscience AG Method of blocking amplification of selected sequences
US7198897B2 (en) * 2001-12-19 2007-04-03 Brandeis University Late-PCR
WO2007039301A2 (en) * 2005-10-05 2007-04-12 Roche Diagnostics Gmbh Non-fluorescent energy transfer
US7803543B2 (en) * 2007-01-19 2010-09-28 Chang Gung University Methods and kits for the detection of nucleotide mutations using peptide nucleic acid as both PCR clamp and sensor probe
US8071338B2 (en) * 2007-08-08 2011-12-06 Roche Molecular Systems, Inc. Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5′-3′ nuclease activity
EP2247601B1 (en) 2008-07-02 2013-04-10 Avexa Limited Thiazopyrimidinones and uses thereof

Also Published As

Publication number Publication date
EP2385991B1 (en) 2017-05-17
CA2747068C (en) 2014-04-08
CN106702000A (zh) 2017-05-24
US8206929B2 (en) 2012-06-26
ES2634428T3 (es) 2017-09-27
JP2012514451A (ja) 2012-06-28
EP2385991A1 (en) 2011-11-16
US20100173311A1 (en) 2010-07-08
CA2747068A1 (en) 2010-07-15
CN102272333A (zh) 2011-12-07
WO2010079127A1 (en) 2010-07-15

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