CN106702000A - 利用等位基因特异性抑制序列变体的核酸扩增 - Google Patents

利用等位基因特异性抑制序列变体的核酸扩增 Download PDF

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Publication number
CN106702000A
CN106702000A CN201710109955.4A CN201710109955A CN106702000A CN 106702000 A CN106702000 A CN 106702000A CN 201710109955 A CN201710109955 A CN 201710109955A CN 106702000 A CN106702000 A CN 106702000A
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CN
China
Prior art keywords
oligonucleotide
target sequence
nucleic acid
variants
amplification
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CN201710109955.4A
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English (en)
Chinese (zh)
Inventor
M.格罗
V.布罗菲
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Publication of CN106702000A publication Critical patent/CN106702000A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201710109955.4A 2009-01-07 2010-01-07 利用等位基因特异性抑制序列变体的核酸扩增 Pending CN106702000A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14311309P 2009-01-07 2009-01-07
US61/143113 2009-01-07
CN2010800043562A CN102272333A (zh) 2009-01-07 2010-01-07 利用等位基因特异性抑制序列变体的核酸扩增

Related Parent Applications (1)

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CN2010800043562A Division CN102272333A (zh) 2009-01-07 2010-01-07 利用等位基因特异性抑制序列变体的核酸扩增

Publications (1)

Publication Number Publication Date
CN106702000A true CN106702000A (zh) 2017-05-24

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ID=41682254

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201710109955.4A Pending CN106702000A (zh) 2009-01-07 2010-01-07 利用等位基因特异性抑制序列变体的核酸扩增
CN2010800043562A Pending CN102272333A (zh) 2009-01-07 2010-01-07 利用等位基因特异性抑制序列变体的核酸扩增

Family Applications After (1)

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CN2010800043562A Pending CN102272333A (zh) 2009-01-07 2010-01-07 利用等位基因特异性抑制序列变体的核酸扩增

Country Status (7)

Country Link
US (1) US8206929B2 (enExample)
EP (1) EP2385991B1 (enExample)
JP (1) JP5785095B2 (enExample)
CN (2) CN106702000A (enExample)
CA (1) CA2747068C (enExample)
ES (1) ES2634428T3 (enExample)
WO (1) WO2010079127A1 (enExample)

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WO2013010074A1 (en) 2011-07-13 2013-01-17 Primeradx, Inc. Multimodal methods for simultaneous detection and quantification of multiple nucleic acids in a sample
GB201116876D0 (en) 2011-09-30 2011-11-16 Epistem Ltd Mutational analysis of JAK2
EP2770065B1 (en) 2013-02-25 2017-12-13 Seegene, Inc. Detection of nucleotide variation on target nucleic acid sequence
JP6050555B2 (ja) 2013-07-15 2016-12-21 シージーン アイエヌシー Ｐｔｏの切断及び延長−依存的固定化オリゴヌクレオチドハイブリダイゼーションを用いたターゲット核酸配列の検出
US10913977B2 (en) 2013-07-24 2021-02-09 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable enrichment of minor DNA alleles by limiting denaturation time in PCR or simply enable enrichment of minor DNA alleles by limiting the denaturation time in PCR
EP3058105B1 (en) 2013-10-18 2019-05-22 Seegene, Inc. Detection of target nucleic acid sequence on solid phase by pto cleavage and extension using hcto assay
JP2016535987A (ja) 2013-10-20 2016-11-24 トローバジーン インコーポレイテッド 核酸配列の合成及び濃縮
KR102343605B1 (ko) * 2014-05-19 2021-12-27 윌리엄 마쉬 라이스 유니버시티 비대립 유전자-특이적 프라이머 및 대립 유전자-특이적 블로커 올리고뉴클레오티드를 중첩하는 조성물을 사용한 대립 유전자-특이적 증폭
JP5813263B1 (ja) * 2015-03-31 2015-11-17 日鉄住金環境株式会社 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド
CA3046953A1 (en) 2016-12-12 2018-06-21 Dana Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
US11174511B2 (en) 2017-07-24 2021-11-16 Dana-Farber Cancer Institute, Inc. Methods and compositions for selecting and amplifying DNA targets in a single reaction mixture
CN117858956A (zh) * 2021-08-25 2024-04-09 南京金斯瑞生物科技有限公司 一种用于检测单碱基突变的引物组和基因芯片方法
JP2024531713A (ja) * 2021-09-13 2024-08-29 ネクスジェン キャンサー ディテクション エルエルシー 試料中の標的ポリヌクレオチドを検出するための方法

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CN1471588A (zh) * 2000-08-23 2004-01-28 宝生物工程株式会社 核酸扩增方法
CN1810990A (zh) * 1999-03-19 2006-08-02 宝生物工程株式会社 用于扩增核酸序列的方法

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CN1810990A (zh) * 1999-03-19 2006-08-02 宝生物工程株式会社 用于扩增核酸序列的方法
CN1471588A (zh) * 2000-08-23 2004-01-28 宝生物工程株式会社 核酸扩增方法

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Also Published As

Publication number Publication date
WO2010079127A1 (en) 2010-07-15
ES2634428T3 (es) 2017-09-27
US20100173311A1 (en) 2010-07-08
US8206929B2 (en) 2012-06-26
CN102272333A (zh) 2011-12-07
JP5785095B2 (ja) 2015-09-24
CA2747068A1 (en) 2010-07-15
EP2385991B1 (en) 2017-05-17
EP2385991A1 (en) 2011-11-16
JP2012514451A (ja) 2012-06-28
CA2747068C (en) 2014-04-08

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