CN106702000A - 利用等位基因特异性抑制序列变体的核酸扩增 - Google Patents

利用等位基因特异性抑制序列变体的核酸扩增 Download PDF

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Publication number
CN106702000A
CN106702000A CN201710109955.4A CN201710109955A CN106702000A CN 106702000 A CN106702000 A CN 106702000A CN 201710109955 A CN201710109955 A CN 201710109955A CN 106702000 A CN106702000 A CN 106702000A
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CN
China
Prior art keywords
oligonucleotide
target sequence
nucleic acid
variants
amplification
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CN201710109955.4A
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English (en)
Chinese (zh)
Inventor
M.格罗
V.布罗菲
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Publication of CN106702000A publication Critical patent/CN106702000A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201710109955.4A 2009-01-07 2010-01-07 利用等位基因特异性抑制序列变体的核酸扩增 Pending CN106702000A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14311309P 2009-01-07 2009-01-07
US61/143113 2009-01-07
CN2010800043562A CN102272333A (zh) 2009-01-07 2010-01-07 利用等位基因特异性抑制序列变体的核酸扩增

Related Parent Applications (1)

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CN2010800043562A Division CN102272333A (zh) 2009-01-07 2010-01-07 利用等位基因特异性抑制序列变体的核酸扩增

Publications (1)

Publication Number Publication Date
CN106702000A true CN106702000A (zh) 2017-05-24

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
CN201710109955.4A Pending CN106702000A (zh) 2009-01-07 2010-01-07 利用等位基因特异性抑制序列变体的核酸扩增
CN2010800043562A Pending CN102272333A (zh) 2009-01-07 2010-01-07 利用等位基因特异性抑制序列变体的核酸扩增

Family Applications After (1)

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CN2010800043562A Pending CN102272333A (zh) 2009-01-07 2010-01-07 利用等位基因特异性抑制序列变体的核酸扩增

Country Status (7)

Country Link
US (1) US8206929B2 (enExample)
EP (1) EP2385991B1 (enExample)
JP (1) JP5785095B2 (enExample)
CN (2) CN106702000A (enExample)
CA (1) CA2747068C (enExample)
ES (1) ES2634428T3 (enExample)
WO (1) WO2010079127A1 (enExample)

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GB201116876D0 (en) 2011-09-30 2011-11-16 Epistem Ltd Mutational analysis of JAK2
ES2662825T3 (es) 2013-02-25 2018-04-09 Seegene, Inc. Detección de variación de nucleótido en una secuencia de ácidos nucleicos diana
WO2015008985A1 (en) 2013-07-15 2015-01-22 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent immobilized oligonucleotide hybridization
US10913977B2 (en) 2013-07-24 2021-02-09 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable enrichment of minor DNA alleles by limiting denaturation time in PCR or simply enable enrichment of minor DNA alleles by limiting the denaturation time in PCR
KR101757473B1 (ko) 2013-10-18 2017-07-13 주식회사 씨젠 ｈＣＴＯ를 이용하는 ＰＴＯ 절단 및 연장 분석에 의한 고상에서의 타겟 핵산 서열 검출
US20160273022A1 (en) 2013-10-20 2016-09-22 Trovagene, Inc. Synthesis and enrichment of nucleic acid sequences
EP4512908A3 (en) * 2014-05-19 2025-03-26 William Marsh Rice University Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides
JP5813263B1 (ja) * 2015-03-31 2015-11-17 日鉄住金環境株式会社 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド
EP3551756A4 (en) 2016-12-12 2020-07-15 Dana Farber Cancer Institute, Inc. COMPOSITIONS AND METHODS FOR MOLECULAR BARCODE CODING OF DNA MOLECULES BEFORE ENRICHMENT OF MUTATIONS AND / OR DETECTION OF MUTATIONS
WO2019023243A1 (en) 2017-07-24 2019-01-31 Dana-Farber Cancer Institute, Inc. METHODS AND COMPOSITIONS FOR SELECTING AND AMPLIFYING DNA TARGETS IN A SINGLE REACTION MIXTURE
US20250092445A1 (en) * 2021-08-25 2025-03-20 Manjing Genscript Biotech Co., Ltd. Primer set and gene chip method for detecting single base mutation
JP2024531713A (ja) * 2021-09-13 2024-08-29 ネクスジェン キャンサー ディテクション エルエルシー 試料中の標的ポリヌクレオチドを検出するための方法

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CN1471588A (zh) * 2000-08-23 2004-01-28 宝生物工程株式会社 核酸扩增方法
CN1810990A (zh) * 1999-03-19 2006-08-02 宝生物工程株式会社 用于扩增核酸序列的方法

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US6001611A (en) * 1997-03-20 1999-12-14 Roche Molecular Systems, Inc. Modified nucleic acid amplification primers
CN1810990A (zh) * 1999-03-19 2006-08-02 宝生物工程株式会社 用于扩增核酸序列的方法
CN1471588A (zh) * 2000-08-23 2004-01-28 宝生物工程株式会社 核酸扩增方法

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Also Published As

Publication number Publication date
WO2010079127A1 (en) 2010-07-15
CN102272333A (zh) 2011-12-07
CA2747068C (en) 2014-04-08
JP2012514451A (ja) 2012-06-28
EP2385991A1 (en) 2011-11-16
JP5785095B2 (ja) 2015-09-24
CA2747068A1 (en) 2010-07-15
US20100173311A1 (en) 2010-07-08
US8206929B2 (en) 2012-06-26
EP2385991B1 (en) 2017-05-17
ES2634428T3 (es) 2017-09-27

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