CA2747068C - Nucleic acid amplification with allele-specific suppression of sequence variants - Google Patents

Nucleic acid amplification with allele-specific suppression of sequence variants Download PDF

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Publication number
CA2747068C
CA2747068C CA2747068A CA2747068A CA2747068C CA 2747068 C CA2747068 C CA 2747068C CA 2747068 A CA2747068 A CA 2747068A CA 2747068 A CA2747068 A CA 2747068A CA 2747068 C CA2747068 C CA 2747068C
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Prior art keywords
oligonucleotide
target sequence
nucleic acid
amplification
variant
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CA2747068A
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English (en)
French (fr)
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CA2747068A1 (en
Inventor
Michael Grow
Victoria Brophy
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA2747068A 2009-01-07 2010-01-07 Nucleic acid amplification with allele-specific suppression of sequence variants Active CA2747068C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14311309P 2009-01-07 2009-01-07
US61/143,113 2009-01-07
PCT/EP2010/000030 WO2010079127A1 (en) 2009-01-07 2010-01-07 Nucleic acid amplification with allele-specific suppression of sequence variants

Publications (2)

Publication Number Publication Date
CA2747068A1 CA2747068A1 (en) 2010-07-15
CA2747068C true CA2747068C (en) 2014-04-08

Family

ID=41682254

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2747068A Active CA2747068C (en) 2009-01-07 2010-01-07 Nucleic acid amplification with allele-specific suppression of sequence variants

Country Status (7)

Country Link
US (1) US8206929B2 (enExample)
EP (1) EP2385991B1 (enExample)
JP (1) JP5785095B2 (enExample)
CN (2) CN106702000A (enExample)
CA (1) CA2747068C (enExample)
ES (1) ES2634428T3 (enExample)
WO (1) WO2010079127A1 (enExample)

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WO2015008985A1 (en) 2013-07-15 2015-01-22 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent immobilized oligonucleotide hybridization
US10913977B2 (en) 2013-07-24 2021-02-09 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable enrichment of minor DNA alleles by limiting denaturation time in PCR or simply enable enrichment of minor DNA alleles by limiting the denaturation time in PCR
KR101757473B1 (ko) 2013-10-18 2017-07-13 주식회사 씨젠 ｈＣＴＯ를 이용하는 ＰＴＯ 절단 및 연장 분석에 의한 고상에서의 타겟 핵산 서열 검출
US20160273022A1 (en) 2013-10-20 2016-09-22 Trovagene, Inc. Synthesis and enrichment of nucleic acid sequences
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JP5813263B1 (ja) * 2015-03-31 2015-11-17 日鉄住金環境株式会社 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド
EP3551756A4 (en) 2016-12-12 2020-07-15 Dana Farber Cancer Institute, Inc. COMPOSITIONS AND METHODS FOR MOLECULAR BARCODE CODING OF DNA MOLECULES BEFORE ENRICHMENT OF MUTATIONS AND / OR DETECTION OF MUTATIONS
WO2019023243A1 (en) 2017-07-24 2019-01-31 Dana-Farber Cancer Institute, Inc. METHODS AND COMPOSITIONS FOR SELECTING AND AMPLIFYING DNA TARGETS IN A SINGLE REACTION MIXTURE
US20250092445A1 (en) * 2021-08-25 2025-03-20 Manjing Genscript Biotech Co., Ltd. Primer set and gene chip method for detecting single base mutation
JP2024531713A (ja) * 2021-09-13 2024-08-29 ネクスジェン キャンサー ディテクション エルエルシー 試料中の標的ポリヌクレオチドを検出するための方法

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Also Published As

Publication number Publication date
WO2010079127A1 (en) 2010-07-15
CN102272333A (zh) 2011-12-07
JP2012514451A (ja) 2012-06-28
EP2385991A1 (en) 2011-11-16
CN106702000A (zh) 2017-05-24
JP5785095B2 (ja) 2015-09-24
CA2747068A1 (en) 2010-07-15
US20100173311A1 (en) 2010-07-08
US8206929B2 (en) 2012-06-26
EP2385991B1 (en) 2017-05-17
ES2634428T3 (es) 2017-09-27

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