CA2747068C - Nucleic acid amplification with allele-specific suppression of sequence variants - Google Patents

Nucleic acid amplification with allele-specific suppression of sequence variants Download PDF

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Publication number
CA2747068C
CA2747068C CA2747068A CA2747068A CA2747068C CA 2747068 C CA2747068 C CA 2747068C CA 2747068 A CA2747068 A CA 2747068A CA 2747068 A CA2747068 A CA 2747068A CA 2747068 C CA2747068 C CA 2747068C
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Canada
Prior art keywords
oligonucleotide
target sequence
nucleic acid
amplification
variant
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CA2747068A
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English (en)
French (fr)
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CA2747068A1 (en
Inventor
Michael Grow
Victoria Brophy
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA2747068A 2009-01-07 2010-01-07 Nucleic acid amplification with allele-specific suppression of sequence variants Active CA2747068C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14311309P 2009-01-07 2009-01-07
US61/143,113 2009-01-07
PCT/EP2010/000030 WO2010079127A1 (en) 2009-01-07 2010-01-07 Nucleic acid amplification with allele-specific suppression of sequence variants

Publications (2)

Publication Number Publication Date
CA2747068A1 CA2747068A1 (en) 2010-07-15
CA2747068C true CA2747068C (en) 2014-04-08

Family

ID=41682254

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2747068A Active CA2747068C (en) 2009-01-07 2010-01-07 Nucleic acid amplification with allele-specific suppression of sequence variants

Country Status (7)

Country Link
US (1) US8206929B2 (enExample)
EP (1) EP2385991B1 (enExample)
JP (1) JP5785095B2 (enExample)
CN (2) CN102272333A (enExample)
CA (1) CA2747068C (enExample)
ES (1) ES2634428T3 (enExample)
WO (1) WO2010079127A1 (enExample)

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GB201116876D0 (en) 2011-09-30 2011-11-16 Epistem Ltd Mutational analysis of JAK2
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US20160244816A1 (en) 2013-07-15 2016-08-25 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent immobilized oligonucleotide hybridization
US10913977B2 (en) 2013-07-24 2021-02-09 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable enrichment of minor DNA alleles by limiting denaturation time in PCR or simply enable enrichment of minor DNA alleles by limiting the denaturation time in PCR
WO2015057008A2 (en) 2013-10-18 2015-04-23 Seegene, Inc. Detection of target nucleic acid sequence on solid phase by pto cleavage and extension using hcto assay
CN105283555A (zh) 2013-10-20 2016-01-27 特罗瓦基因公司 核酸序列的合成和富集
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JP5813263B1 (ja) * 2015-03-31 2015-11-17 日鉄住金環境株式会社 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド
US11371090B2 (en) 2016-12-12 2022-06-28 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of DNA molecules prior to mutation enrichment and/or mutation detection
US11174511B2 (en) 2017-07-24 2021-11-16 Dana-Farber Cancer Institute, Inc. Methods and compositions for selecting and amplifying DNA targets in a single reaction mixture
CN117858956A (zh) * 2021-08-25 2024-04-09 南京金斯瑞生物科技有限公司 一种用于检测单碱基突变的引物组和基因芯片方法
US20240401122A1 (en) * 2021-09-13 2024-12-05 Nexgen Cancer Detection Llc Methods for detecting a target polynucleotide in a sample

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ATE197718T1 (de) 1990-09-28 2000-12-15 Hoffmann La Roche Gereinigte thermostabile nukleinsäurepolymerase aus thermosipho africanus
JPH04234422A (ja) 1990-10-31 1992-08-24 Internatl Business Mach Corp <Ibm> 二重硬化エポキシバックシール処方物
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Also Published As

Publication number Publication date
JP2012514451A (ja) 2012-06-28
WO2010079127A1 (en) 2010-07-15
EP2385991B1 (en) 2017-05-17
EP2385991A1 (en) 2011-11-16
US8206929B2 (en) 2012-06-26
CN102272333A (zh) 2011-12-07
ES2634428T3 (es) 2017-09-27
US20100173311A1 (en) 2010-07-08
JP5785095B2 (ja) 2015-09-24
CA2747068A1 (en) 2010-07-15
CN106702000A (zh) 2017-05-24

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