JP2009513142A5 - - Google Patents
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- Publication number
- JP2009513142A5 JP2009513142A5 JP2008538067A JP2008538067A JP2009513142A5 JP 2009513142 A5 JP2009513142 A5 JP 2009513142A5 JP 2008538067 A JP2008538067 A JP 2008538067A JP 2008538067 A JP2008538067 A JP 2008538067A JP 2009513142 A5 JP2009513142 A5 JP 2009513142A5
- Authority
- JP
- Japan
- Prior art keywords
- population
- nucleic acid
- target
- acid molecules
- hybridizing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108020004707 nucleic acids Proteins 0.000 claims 33
- 102000039446 nucleic acids Human genes 0.000 claims 33
- 150000007523 nucleic acids Chemical class 0.000 claims 33
- 238000000034 method Methods 0.000 claims 29
- 108091034117 Oligonucleotide Proteins 0.000 claims 22
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 12
- 239000002773 nucleotide Substances 0.000 claims 10
- 125000003729 nucleotide group Chemical group 0.000 claims 10
- 238000013518 transcription Methods 0.000 claims 7
- 230000035897 transcription Effects 0.000 claims 7
- 108020004999 messenger RNA Proteins 0.000 claims 6
- 230000003321 amplification Effects 0.000 claims 5
- 239000002299 complementary DNA Substances 0.000 claims 5
- 238000003199 nucleic acid amplification method Methods 0.000 claims 5
- 108020004414 DNA Proteins 0.000 claims 4
- 108060003196 globin Proteins 0.000 claims 4
- 102000018146 globin Human genes 0.000 claims 4
- 230000002194 synthesizing effect Effects 0.000 claims 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 3
- 238000012408 PCR amplification Methods 0.000 claims 3
- 238000000137 annealing Methods 0.000 claims 3
- 210000000601 blood cell Anatomy 0.000 claims 3
- 210000004027 cell Anatomy 0.000 claims 3
- 238000000338 in vitro Methods 0.000 claims 3
- 238000010839 reverse transcription Methods 0.000 claims 3
- 125000006850 spacer group Chemical group 0.000 claims 3
- 102000053602 DNA Human genes 0.000 claims 2
- 235000010469 Glycine max Nutrition 0.000 claims 2
- 244000068988 Glycine max Species 0.000 claims 2
- 102100034343 Integrase Human genes 0.000 claims 2
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims 2
- 108020001027 Ribosomal DNA Proteins 0.000 claims 2
- 108020004418 ribosomal RNA Proteins 0.000 claims 2
- 210000003651 basophil Anatomy 0.000 claims 1
- 210000001772 blood platelet Anatomy 0.000 claims 1
- 210000003979 eosinophil Anatomy 0.000 claims 1
- 210000003743 erythrocyte Anatomy 0.000 claims 1
- 238000009396 hybridization Methods 0.000 claims 1
- 210000004698 lymphocyte Anatomy 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 210000001616 monocyte Anatomy 0.000 claims 1
- 210000000440 neutrophil Anatomy 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US73152905P | 2005-10-27 | 2005-10-27 | |
| US60/731,529 | 2005-10-27 | ||
| US79045806P | 2006-04-07 | 2006-04-07 | |
| US60/790,458 | 2006-04-07 | ||
| PCT/US2006/042250 WO2007050990A2 (en) | 2005-10-27 | 2006-10-27 | Nucleic acid amplification using non-random primers |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2009513142A JP2009513142A (ja) | 2009-04-02 |
| JP2009513142A5 true JP2009513142A5 (enExample) | 2009-12-17 |
| JP5409005B2 JP5409005B2 (ja) | 2014-02-05 |
Family
ID=37968610
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2008538067A Expired - Fee Related JP5409005B2 (ja) | 2005-10-27 | 2006-10-27 | 非ランダムプライマーを用いる核酸増幅 |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US20080187969A1 (enExample) |
| EP (2) | EP1941058A4 (enExample) |
| JP (1) | JP5409005B2 (enExample) |
| CA (1) | CA2626977A1 (enExample) |
| WO (1) | WO2007050990A2 (enExample) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2206779B1 (en) | 2005-12-06 | 2013-02-13 | Life Technologies Corporation | Reverse transcription primers and methods of design |
| MX2009009530A (es) * | 2007-03-07 | 2010-05-19 | Nventa Biopharmaceuticals Corp | Composiciones de acidos nucleicos cerrados bicatenarios. |
| CN102124126A (zh) * | 2007-10-26 | 2011-07-13 | 生命技术公司 | 使用非随机引物的cdna合成 |
| WO2009091719A1 (en) | 2008-01-14 | 2009-07-23 | Applera Corporation | Compositions, methods, and kits for detecting ribonucleic acid |
| EP2285961A4 (en) * | 2008-05-15 | 2012-10-17 | Topigen Pharmaceuticals Inc | OLIGONUCLEOTIDES FOR THE TREATMENT OF INFLAMMATION AND NEOPLASTIC CELL REPRODUCTION |
| US20100159533A1 (en) * | 2008-11-24 | 2010-06-24 | Helicos Biosciences Corporation | Simplified sample preparation for rna analysis |
| EP3181702B1 (en) * | 2008-11-24 | 2019-04-17 | Sequenom, Inc. | Improving nucleic acid quantitation by compressing the dynamic range of high-abundance molecules |
| WO2011032053A1 (en) * | 2009-09-11 | 2011-03-17 | Nugen Technologies, Inc. | Compositions and methods for whole transcriptome analysis |
| WO2012064739A2 (en) * | 2010-11-08 | 2012-05-18 | The Trustees Of Columbia University In The City Of New York | Microbial enrichment primers |
| CN107058059B (zh) | 2011-09-26 | 2020-08-07 | 基因技术股份公司 | 高效的小体积核酸合成 |
| WO2014153188A2 (en) | 2013-03-14 | 2014-09-25 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
| CN107532129B (zh) | 2014-12-09 | 2022-09-13 | 生命技术公司 | 高效小体积核酸合成 |
| EP3974528A4 (en) * | 2019-05-20 | 2022-11-09 | Kyushu University, National University Corporation | OLIGONUCLEOTIDE, METHOD AND OMICS ANALYSIS KIT |
Family Cites Families (61)
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| US5723597A (en) * | 1983-01-10 | 1998-03-03 | Gen-Probe Incorporated | Ribosomal nucleic acid probes for detecting organisms or groups of organisms |
| US6221581B1 (en) * | 1984-04-27 | 2001-04-24 | Enzo Diagnostics, Inc. | Processes for detecting polynucleotides, determining genetic mutations or defects in genetic material, separating or isolating nucleic acid of interest from samples, and useful compositions of matter and multihybrid complex compositions |
| US5541308A (en) * | 1986-11-24 | 1996-07-30 | Gen-Probe Incorporated | Nucleic acid probes for detection and/or quantitation of non-viral organisms |
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| US5639604A (en) * | 1987-09-21 | 1997-06-17 | Gen-Probe Incorporated | Homogeneous protection assay |
| US5084565A (en) * | 1988-08-18 | 1992-01-28 | Gene-Trak Systems | Probes for the specific detection of escherichia coli and shigella |
| US5512439A (en) * | 1988-11-21 | 1996-04-30 | Dynal As | Oligonucleotide-linked magnetic particles and uses thereof |
| US5708154A (en) * | 1989-02-24 | 1998-01-13 | City Of Hope | RNA-DNA hybrid molecules of nucleic acid |
| JP3046838B2 (ja) * | 1989-03-10 | 2000-05-29 | バイシス・インコーポレーテツド | 親和性捕捉法の妨害の防止 |
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| US5744101A (en) * | 1989-06-07 | 1998-04-28 | Affymax Technologies N.V. | Photolabile nucleoside protecting groups |
| US5545522A (en) * | 1989-09-22 | 1996-08-13 | Van Gelder; Russell N. | Process for amplifying a target polynucleotide sequence using a single primer-promoter complex |
| CA2025181A1 (en) * | 1989-10-12 | 1991-04-13 | William G. Weisburg | Nucleic acid probes and methods for detecting fungi |
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| US5633134A (en) * | 1992-10-06 | 1997-05-27 | Ig Laboratories, Inc. | Method for simultaneously detecting multiple mutations in a DNA sample |
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| FR2737502B1 (fr) * | 1995-07-31 | 1997-10-24 | Genset Sa | Procede de detection d'acides nucleiques utilisant des sondes nucleotidiques permettant a la fois une capture specifique et une detection |
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-
2006
- 2006-10-27 WO PCT/US2006/042250 patent/WO2007050990A2/en not_active Ceased
- 2006-10-27 EP EP06827033A patent/EP1941058A4/en not_active Withdrawn
- 2006-10-27 EP EP12165043A patent/EP2479291A1/en not_active Withdrawn
- 2006-10-27 CA CA002626977A patent/CA2626977A1/en not_active Abandoned
- 2006-10-27 JP JP2008538067A patent/JP5409005B2/ja not_active Expired - Fee Related
- 2006-10-27 US US11/589,322 patent/US20080187969A1/en not_active Abandoned
-
2011
- 2011-04-11 US US13/084,342 patent/US20110294701A1/en not_active Abandoned
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