JP2018516594A5 - - Google Patents

Info

Publication number

JP2018516594A5

JP2018516594A5 JP2018512815A JP2018512815A JP2018516594A5 JP 2018516594 A5 JP2018516594 A5 JP 2018516594A5 JP 2018512815 A JP2018512815 A JP 2018512815A JP 2018512815 A JP2018512815 A JP 2018512815A JP 2018516594 A5 JP2018516594 A5 JP 2018516594A5

Authority

JP

Japan

Prior art keywords

nucleotide sequence

nucleic acid

seq

identity

ltr

Prior art date

2016-05-16

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Pending

Links

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• 239000002773 nucleotide Substances 0.000 claims description 152
• 125000003729 nucleotide group Chemical group 0.000 claims description 152
• 150000007523 nucleic acids Chemical class 0.000 claims description 137
• 108020004707 nucleic acids Proteins 0.000 claims description 118
• 102000039446 nucleic acids Human genes 0.000 claims description 118
• 239000000523 sample Substances 0.000 claims description 109
• 238000000034 method Methods 0.000 claims description 75
• 108091093088 Amplicon Proteins 0.000 claims description 28
• 108091028043 Nucleic acid sequence Proteins 0.000 claims description 19
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 12
• 108700026226 TATA Box Proteins 0.000 claims description 11
• 238000001514 detection method Methods 0.000 claims description 9
• 238000000137 annealing Methods 0.000 claims description 6
• QOFZZTBWWJNFCA-UHFFFAOYSA-N texas red-X Chemical compound [O-]S(=O)(=O)C1=CC(S(=O)(=O)NCCCCCC(=O)O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 QOFZZTBWWJNFCA-UHFFFAOYSA-N 0.000 claims description 6
• 230000003321 amplification Effects 0.000 claims description 5
• 238000003199 nucleic acid amplification method Methods 0.000 claims description 5
• 238000003753 real-time PCR Methods 0.000 claims description 5
• 238000000926 separation method Methods 0.000 claims description 2
• 241000713666 Lentivirus Species 0.000 claims 3
• 101100382321 Caenorhabditis elegans cal-1 gene Proteins 0.000 description 9
• 239000013598 vector Substances 0.000 description 4
• 108020004414 DNA Proteins 0.000 description 3
• 238000012239 gene modification Methods 0.000 description 3
• 230000005017 genetic modification Effects 0.000 description 3
• 235000013617 genetically modified food Nutrition 0.000 description 3
• 230000001225 therapeutic effect Effects 0.000 description 3
• 238000011002 quantification Methods 0.000 description 2
• 238000003556 assay Methods 0.000 description 1
• 239000002299 complementary DNA Substances 0.000 description 1
• 230000000694 effects Effects 0.000 description 1
• 239000012634 fragment Substances 0.000 description 1
• 238000003144 genetic modification method Methods 0.000 description 1
• 238000010348 incorporation Methods 0.000 description 1
• 108090000623 proteins and genes Proteins 0.000 description 1
• 238000012546 transfer Methods 0.000 description 1