JP2020533974A5 - - Google Patents

Info

Publication number

JP2020533974A5

JP2020533974A5 JP2020512607A JP2020512607A JP2020533974A5 JP 2020533974 A5 JP2020533974 A5 JP 2020533974A5 JP 2020512607 A JP2020512607 A JP 2020512607A JP 2020512607 A JP2020512607 A JP 2020512607A JP 2020533974 A5 JP2020533974 A5 JP 2020533974A5

Authority

JP

Japan

Prior art keywords

nicking

nicking enzyme

nucleotide sequence

site

template

Prior art date

2018-08-30

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Pending

Links

• Espacenet
• Global Dossier
• Discuss

• 238000000034 method Methods 0.000 claims 25
• 108090000790 Enzymes Proteins 0.000 claims 22
• 102000004190 Enzymes Human genes 0.000 claims 22
• 125000003729 nucleotide group Chemical group 0.000 claims 18
• 239000002773 nucleotide Substances 0.000 claims 15
• 230000003321 amplification Effects 0.000 claims 8
• 238000003199 nucleic acid amplification method Methods 0.000 claims 8
• 150000007523 nucleic acids Chemical group 0.000 claims 8
• 238000011144 upstream manufacturing Methods 0.000 claims 8
• 239000012472 biological sample Substances 0.000 claims 7
• 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 6
• 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 6
• 230000000295 complement effect Effects 0.000 claims 6
• 239000000203 mixture Substances 0.000 claims 6
• 101150095629 NS2 gene Proteins 0.000 claims 5
• 108091028043 Nucleic acid sequence Proteins 0.000 claims 5
• 108090001074 Nucleocapsid Proteins Proteins 0.000 claims 5
• 239000000523 sample Substances 0.000 claims 5
• 108091034117 Oligonucleotide Proteins 0.000 claims 4
• 108091081021 Sense strand Proteins 0.000 claims 4
• 230000000692 anti-sense effect Effects 0.000 claims 4
• 230000006641 stabilisation Effects 0.000 claims 4
• 238000011105 stabilization Methods 0.000 claims 4
• 108020004707 nucleic acids Proteins 0.000 claims 3
• 102000039446 nucleic acids Human genes 0.000 claims 3
• 238000006243 chemical reaction Methods 0.000 claims 2
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
• 241000626621 Geobacillus Species 0.000 claims 1
• 241000211181 Manta Species 0.000 claims 1
• 206010036790 Productive cough Diseases 0.000 claims 1
• 230000000903 blocking effect Effects 0.000 claims 1
• 239000008280 blood Substances 0.000 claims 1
• 210000004369 blood Anatomy 0.000 claims 1
• 230000002255 enzymatic effect Effects 0.000 claims 1
• 238000006911 enzymatic reaction Methods 0.000 claims 1
• 239000002532 enzyme inhibitor Substances 0.000 claims 1
• 210000003608 fece Anatomy 0.000 claims 1
• 239000012634 fragment Substances 0.000 claims 1
• NFGXHKASABOEEW-LDRANXPESA-N methoprene Chemical compound COC(C)(C)CCCC(C)C\C=C\C(\C)=C\C(=O)OC(C)C NFGXHKASABOEEW-LDRANXPESA-N 0.000 claims 1
• 238000012986 modification Methods 0.000 claims 1
• 230000004048 modification Effects 0.000 claims 1
• 210000004915 pus Anatomy 0.000 claims 1
• 210000003296 saliva Anatomy 0.000 claims 1
• 125000006850 spacer group Chemical group 0.000 claims 1
• 210000003802 sputum Anatomy 0.000 claims 1
• 208000024794 sputum Diseases 0.000 claims 1
• 210000002700 urine Anatomy 0.000 claims 1