BR112020004071A2 - reação de amplificação por nicking e extensão (near) de espécies do vírus sincicial respiratório - Google Patents

reação de amplificação por nicking e extensão (near) de espécies do vírus sincicial respiratório Download PDF

Info

Publication number
BR112020004071A2
BR112020004071A2 BR112020004071-1A BR112020004071A BR112020004071A2 BR 112020004071 A2 BR112020004071 A2 BR 112020004071A2 BR 112020004071 A BR112020004071 A BR 112020004071A BR 112020004071 A2 BR112020004071 A2 BR 112020004071A2
Authority
BR
Brazil
Prior art keywords
rsv
nicking
nucleotide sequence
sequence
gene
Prior art date
Application number
BR112020004071-1A
Other languages
English (en)
Portuguese (pt)
Inventor
Honghua Zhang
Richard Roth
Original Assignee
Ionian Technologies, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ionian Technologies, Inc. filed Critical Ionian Technologies, Inc.
Publication of BR112020004071A2 publication Critical patent/BR112020004071A2/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701Specific hybridization probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/11Antisense
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/50Other enzymatic activities
    • C12Q2521/507Recombinase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/16Primer sets for multiplex assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/166Oligonucleotides used as internal standards, controls or normalisation probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
BR112020004071-1A 2017-08-31 2018-08-30 reação de amplificação por nicking e extensão (near) de espécies do vírus sincicial respiratório BR112020004071A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762552546P 2017-08-31 2017-08-31
US62/552,546 2017-08-31
PCT/US2018/048903 WO2019046610A1 (en) 2017-08-31 2018-08-30 REACTION OF SYNCHRONIZATION AMPLIFICATION AND (NEAR) EXTENSION OF SYNCYTIAL RESPIRATORY VIRUS SPECIES

Publications (1)

Publication Number Publication Date
BR112020004071A2 true BR112020004071A2 (pt) 2020-09-24

Family

ID=65526105

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112020004071-1A BR112020004071A2 (pt) 2017-08-31 2018-08-30 reação de amplificação por nicking e extensão (near) de espécies do vírus sincicial respiratório

Country Status (10)

Country Link
US (1) US11634770B2 (enExample)
EP (1) EP3676394A4 (enExample)
JP (1) JP2020533974A (enExample)
KR (1) KR20200083444A (enExample)
CN (1) CN111406118B (enExample)
AU (1) AU2018326627C1 (enExample)
BR (1) BR112020004071A2 (enExample)
CA (1) CA3073954A1 (enExample)
RU (1) RU2020100221A (enExample)
WO (1) WO2019046610A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9689031B2 (en) * 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
GB201611469D0 (en) 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes
GB2569965A (en) 2018-01-04 2019-07-10 Lumiradx Uk Ltd Improvements in or relating to amplification of nucleic acids
WO2025030178A1 (en) * 2023-08-03 2025-02-06 Abbott Laboratories Isothermal amplification of nucleic acids

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060287267A1 (en) 2001-05-18 2006-12-21 Sirna Therapeutics, Inc. RNA interference mediated inhibition of respiratory syncytial virus (RSV) expression using short interfering nucleic acid (siNA)
WO2004022701A2 (en) * 2001-07-15 2004-03-18 Keck Graduate Institute Exponential amplification of nucleic acids using nicking agents
US6881835B2 (en) * 2002-01-04 2005-04-19 Dr. Chip Biotechnology Inc. Detection of respiratory viruses
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
AU2003215391B2 (en) 2002-02-21 2007-05-24 Abbott Diagnostics Scarborough, Inc. Recombinase Polymerase Amplification
CA2616241C (en) * 2005-07-25 2012-02-07 Asm Scientific, Inc. Methods for multiplexing recombinase polymerase amplification
CA2650993C (en) 2006-05-04 2015-06-16 Asm Scientific, Inc. Recombinase polymerase amplification
US9689031B2 (en) * 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
EP2438196B1 (en) 2009-06-05 2016-12-21 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
BR112014025262A8 (pt) * 2012-04-09 2021-06-29 Envirologix Inc “métodos para quantificar um produto específico em uma reação de amplificação de extensão e corte, para detectar uma pluralidade de produtos de reação distintos produzidos ao longo de uma única reação, para monitorar em tempo real uma reação de amplificação de extensão e corte, para monitorar em tempo real uma molécula-alvo de ácido nucleico em uma reação de amplificação de extensão e corte, para monitorar em tempo real uma molécula-alvo de ácido nucleico em uma amostra de teste, e para amplificar um polinucleotídeo, kit, e, oligonucleotídeo isolado
US10329601B2 (en) * 2015-12-28 2019-06-25 Ionian Technologies, Inc. Nicking and extension amplification reaction (NEAR) of Streptococcus species

Also Published As

Publication number Publication date
EP3676394A4 (en) 2021-06-02
CA3073954A1 (en) 2019-03-07
WO2019046610A1 (en) 2019-03-07
US20190194747A1 (en) 2019-06-27
KR20200083444A (ko) 2020-07-08
AU2018326627A1 (en) 2020-01-30
JP2020533974A (ja) 2020-11-26
CN111406118A (zh) 2020-07-10
AU2018326627C1 (en) 2024-12-12
US11634770B2 (en) 2023-04-25
EP3676394A1 (en) 2020-07-08
AU2018326627B2 (en) 2024-08-22
RU2020100221A (ru) 2021-09-30
CN111406118B (zh) 2024-07-12
RU2020100221A3 (enExample) 2022-03-11

Similar Documents

Publication Publication Date Title
US11186864B2 (en) Nicking and extension amplification reaction (near) of Streptococcus species
US8354230B2 (en) Multiplex detection assay for influenza and RSV viruses
Yu et al. Development of a real-time reverse transcription loop-mediated isothermal amplification method for the rapid detection of porcine epidemic diarrhea virus
BR112014000138A2 (pt) Método para identificar a presença ou ausência de um ácido nucleico alvo
BR112020004071A2 (pt) reação de amplificação por nicking e extensão (near) de espécies do vírus sincicial respiratório
TW202219272A (zh) SARS-CoV-2之偵測
EP4163397A1 (en) Specimen transport kit for detecting respiratory pathogens and methods for detecting respiratory pathogens using same
Fan et al. A rapid and highly sensitive multiple detection of human adenovirus type 3, type 7 and respiratory syncytial virus by recombinase‐aided reverse transcription PCR
Cea-Callejo et al. An affordable detection system based on RT-LAMP and DNA-nanoprobes for avian metapneumovirus
CN106471135A (zh) 肠道病毒和双埃柯病毒的分子检测
BR112015022470B1 (pt) Sonda ou iniciador de oligonucleotídeo, método para determinar presença da sequência do gene de proteína externa principal (opca) de neisseria gonorrhoeae e composições para detecção de neisseria gonorrhoeae
Shang et al. Duplex Reverse Transcription Multienzyme Isothermal Rapid Amplification Assays for Detecting SARS-CoV-2.
US20240124947A1 (en) Compositions for coronavirus detection and methods of making and using therof
US20210340635A1 (en) Materials and methods for detecting coronavirus
CN101178350B (zh) 检测狂犬病毒的试剂盒
JP6181660B2 (ja) 血中のストレプトコッカス・ニューモニエの検出
Matthews et al. Semiquantitative polymerase chain reaction enzyme immunoassay for the diagnosis of pertussis
Cea et al. Development of a Fast and Affordable Diagnostic System for RNA Viruses Using Loop-Mediated Isothermal Amplification and DNA-Nanoprobe Detection
KR102468964B1 (ko) 뎅기, 지카 및 치쿤구니야를 포함한 다중 모기 매개 감염병 진단용 프라이머 세트와 이를 이용한 동시다중 분자진단 방법과 랩온페이퍼 기반 진단 키트
HK40025966A (en) Nicking and extension amplification reaction (near) of respiratory syncytial virus species
US9157128B2 (en) Kit for detecting HIV-2 and method for detecting HIV-2 using the same
Koentjoro et al. RNA Internal Control (IC) for Routine Clinical Diagnostic Real-Time Reverse Transcription-PCR SARS-CoV-2
Irawati et al. Concordance between Isothermal Amplification-Real Time Fluorescence Assay and Reverse Transcriptase Real Time Polymerase Chain Reaction for Detecting SARS-CoV-2
SARS-CoV RNA Internal Control (IC) for Routine Clinical
JP2024505686A (ja) ヒトパラインフルエンザウイルス1-4(hpiv1-4)の検出のための組成物及び方法

Legal Events

Date Code Title Description
B350 Update of information on the portal [chapter 15.35 patent gazette]
B06W Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements