TW202219272A - SARS-CoV-2之偵測 - Google Patents
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Abstract
本文提供用於偵測SARS-CoV-2之方法及組成物。
Description
相關申請案之交叉引用
本申請案主張於2020年6月12日提出申請之美國臨時專利申請案第63/038,715號；於2020年7月20日提出申請之美國臨時專利申請案第63/054,214號；於2020年7月24日提出申請之美國臨時專利申請案第63/056,523號；於2020年8月21日提出申請之美國臨時專利申請案第63/068,817號；於2021年1月19日提出申請之美國臨時專利申請案第63/139,268號；於2021年5月6日提出申請之美國臨時專利申請案第63/185,268號中每一者之優先權，該等美國臨時專利申請案中每一者之全部內容皆以引用方式併入本文中。
於2019年12月首次在人類中鑑別出之SARS-CoV-2導致新冠病毒肺炎(COVID-19)，且由世界衛生組織(World Health Organization)在2020年3月11日宣佈為全球大流行。任何及所有爆發之生產性公共衛生管控之標誌係對個體進行測試以鑑別其感染狀況之能力。目前迫切需要改良之偵測及診斷技術。
本文提供用於偵測及診斷SARS-CoV-2之組成物及方法。
定義
約 ：術語「約」在本文中用於提及值時係指在上下文中與所提及值相似之值。一般而言，熟悉上下文之熟習此項技術者應了解在該該上下文中「約」所涵蓋之相關變化程度。舉例而言，在一些實施例中，術語「約」可涵蓋在所提及值之25%、20%、19%、18%、17%、16%、15%、14%、13%、12%、11%、10%、9%、8%、7%、6%、5%、4%、3%、2%、1%或更小之內的一系列值。
劑 ：一般而言，如本文所用之術語「劑」用於指實體(例如脂質、金屬、核酸、多肽、多糖、小分子等，或其複合物、組合、混合物或系統[例如細胞、組織、生物體])或現象(例如熱、電流或電場、磁力或磁場等)。在適當情況下，如熟習此項技術者根據上下文將清楚，該術語可用於指為或包含細胞或生物體或其部分、提取物或組分之實體。替代地或另外，如上下文將表明，該術語可用於指在自然界中發現及/或自自然界獲得之天然產物。在一些情況下，同樣如上下文將表明，該術語可用於指經由人手之動作設計、改造及/或產生及/或並非在自然界發現之一或多種人造實體。在一些實施例中，劑可以經分離或純形式使用；在一些實施例中，劑可以粗形式使用。在一些實施例中，潛在劑可提供為例如可經篩選以鑑別或表徵其內之活性劑之集合或文庫。在一些情形下，術語「劑」可指為或包含聚合物之化合物或實體；在一些情形下，該術語可指包含一或多個聚合物部分之化合物或實體。在一些實施例中，術語「劑」可指不為聚合物及/或實質上不含任何聚合物及/或一或多個特定聚合物部分之化合物或實體。在一些實施例中，該術語可指缺少或實質上不含任何聚合物部分之化合物或實體。
胺基酸 ：在其最廣泛含義中，如本文所用，係指可例如經由形成一或多個肽鍵納入多肽鏈中之任何化合物及/或物質。在一些實施例中，胺基酸具有一般結構H2N-C(H)(R)-COOH。 在一些實施例中，胺基酸係天然胺基酸。在一些實施例中，胺基酸係非天然胺基酸；在一些實施例中，胺基酸係D-胺基酸；在一些實施例中，胺基酸係L-胺基酸。「標準胺基酸」係指在天然肽中通常發現之20種標準L-胺基酸中之任一者。「非標準胺基酸」係指除標準胺基酸外之任一胺基酸，無論其係以合成方式製備抑或自天然來源獲得。在一些實施例中，與上述一般結構相比，胺基酸(包括多肽中之羧基末端及/或胺基末端胺基酸)可含有結構修飾。舉例而言，在一些實施例中，與一般結構相比，胺基酸可藉由甲基化、醯胺化、乙醯化、聚乙二醇化、糖基化、磷酸化及/或取代(例如胺基、羧酸基、一或多個質子及/或羥基)來修飾。在一些實施例中，與含有原本相同之未經修飾之胺基酸之多肽相比，此修飾可例如改變含有經修飾胺基酸之多肽之循環半衰期。在一些實施例中，與含有原本相同之未經修飾之胺基酸之多肽相比，此修飾不會顯著改變含有經修飾胺基酸之多肽之相關活性。如根據上下文將清楚，在一些實施例中，術語「胺基酸」可用於指游離胺基酸；在一些實施例中，其可用於指多肽之胺基酸殘基。
大約 ：如本文所用之術語「大約」或「約」在應用於一或多個所關注值時係指與所述參考值相似之值。在某些實施例中，除非另有說明或自上下文明顯看出，否則術語「大約」或「約」係指在任一方向上(大於或小於)在所述參考值之25%、20%、19%、18%、17%、16%、15%、14%、13%、12%、11%、10%、9%、8%、7%、6%、5%、4%、3%、2%、1%或更小內之一系列值(此數值將超過可能值之100%的情況除外)。
相關 ：當該術語用於本文中時，若一個事件或實體之存在、水準、程度、類型及/或形式與另一個事件或實體之存在、水準、程度、類型及/或形式相關聯，則兩個事件或實體彼此「相關」。舉例而言，若特定實體(例如多肽、遺傳特徵、代謝物、微生物等)之存在、水準及/或形式與疾病、病症或病狀之發生率及/或易感率(例如在相關群體中)相關聯，則認為該特定實體與特定疾病、病症或病狀相關。在一些實施例中，若兩個或更多個實體直接或間接相互作用，以使得其在物理上彼此靠近及/或保持靠近，則該兩個或更多個實體在物理上彼此「相關」。在一些實施例中，兩個或更多個在物理上彼此相關之實體彼此共價連接；在一些實施例中，兩個或更多個在物理上彼此相關之實體彼此不共價連接，但例如藉助氫鍵、范德華(van der Waals)相互作用、疏水相互作用、磁性及其組合非共價相關。
結合 ：應理解，如本文所用之術語「結合」通常係指兩個或更多個實體之間之非共價締合。「直接」結合涉及實體或部分之間之物理接觸；間接結合涉及藉助與一或多個中間實體物理接觸之物理相互作用。兩個或更多個實體之間之結合通常可在各種上下文中之任一者中來評價，包括在分離或在更複雜系統(例如同時與載體實體共價或以其他方式締合及/或在生物系統或細胞中)之上下文中研究相互作用實體或部分的情況。
生物樣品 ： 如本文所用之術語「生物樣品」通常係指自所關注生物來源(例如組織或生物體或細胞培養物)獲得或衍生而來之樣品，如本文所述。在一些實施例中，所關注來源係或包含生物體，例如動物或人類。在一些實施例中，生物樣品係或包含生物組織或流體。在一些實施例中，生物樣品可為或包含骨髓；血液；血球；腹水；組織或細針生檢樣品；含細胞之體液；自由浮動之核酸；痰；唾液；尿；腦脊液、腹膜液；肋膜液；糞便；淋巴；婦科液；皮膚拭子；陰道拭子；口腔拭子；鼻拭子；洗滌液或灌洗液，例如管灌洗液或支氣管肺泡灌洗液；吸出物；刮取物；骨髓樣本；組織生檢樣本；手術樣本；糞便、其他體液、分泌物及/或排泄物；及/或來自其之細胞 等。在一些實施例中，生物樣品係或包含自個體獲得之細胞。在一些實施例中，所獲得細胞係或包括來自獲得樣品之個體之細胞。在一些實施例中，樣品係藉由任何適當方式直接自所關注來源獲得之「初級樣品」。舉例而言，在一些實施例中，初級生物樣品係藉由選自由生檢(例如細針吸出或組織生檢)、手術、收集體液(例如 血液、淋巴、糞便 等) 等組成之群組之方法獲得。在一些實施例中，如根據上下文應清楚，術語「樣品」係指藉由處理初級樣品(例如藉由去除初級樣品之一或多種組分及/或藉由向初級樣品中添加一或多種劑)獲得之製劑。舉例而言，使用半透膜過濾。此一「經處理樣品」可包含例如自樣品提取或藉由使初級樣品經受諸如mRNA之擴增或反轉錄、某些組分之分離及/或純化等技術獲得之核酸或蛋白質 。
細胞溶解物 ：如本文所用之術語「細胞溶解物(cellular lysate)」或「細胞溶解物(cell lysate)」係指含有一或多個經破壞細胞(即膜已經破壞之細胞)之內容物之流體。在一些實施例中，細胞溶解物包括親水性及疏水性細胞組分。在一些實施例中，細胞溶解物主要包括親水性組分；在一些實施例中，細胞溶解物主要包括疏水性組分。在一些實施例中，細胞溶解物係一或多種選自由以下組成之群組之細胞之溶解物：植物細胞、微生物(例如細菌或真菌)細胞、動物細胞(例如哺乳動物細胞)、人類細胞及其組合。在一些實施例中，細胞溶解物係一或多種異常細胞(例如癌細胞)之溶解物。在一些實施例中，細胞溶解物係在破壞細胞後實施極少或未實施純化之粗溶解物；在一些實施例中，此一溶解物稱為「初級」溶解物。在一些實施例中，對初級溶解物實施一或多個分離或純化步驟；然而，術語「溶解物」係指包括多個細胞組分之製劑而非任一個別組分之純製劑。
組成物 ：熟習此項技術者應了解，如本文所用之術語「組成物」可用於指包含一或多種指定組分之離散物理實體。一般而言，除非另有說明，否則組成物可具有任一形式，例如氣體、凝膠、液體、固體等。
包含 ：本文闡述為「包含」一或多個命名要素或步驟之組成物或方法具有開放性，此意指命名要素或步驟係必需的，但可在組成物或方法之範圍內添加其他要素或步驟。為避免冗長，亦應理解，闡述為「包含(comprising)」(或其「包含(comprises)」)一或多個命名要素或步驟之任一組成物或方法亦闡述「基本上由相同命名要素或步驟組成(consisting essentially of)」(或其「基本上由相同命名要素或步驟組成(consists essentially of)」)之相應更有限之組成物或方法，此意指組成物或方法包括命名的必需要素或步驟且亦可包括不會實質上影響組成物或方法之基本及新穎特徵之其他要素或步驟。亦應理解，本文闡述為「包含(comprising)一或多個命名要素或步驟」或「基本上由一或多個命名要素或步驟組成(consisting essentially of)」之任一組成物或方法亦闡述「由命名要素或步驟組成(consisting of)」(或「由命名要素或步驟組成(consists of)」)之相應更有限且封閉的組成物或方法，以排除任何其他未命名之要素或步驟。在本文所揭示之任一組成物或方法中，可用任一命名的必需要素或步驟之已知或所揭示等效物取代該要素或步驟。
對應於 ：如本文所用之術語「對應於」可用於經由與適當參考化合物或組成物比較來表示結構要素在化合物或組成物中之位置/屬性。舉例而言，在一些實施例中，聚合物中之單體殘基(例如多肽中之胺基酸殘基或多核苷酸中之核酸殘基)可鑑別為「對應於」適當參考聚合物中之殘基。舉例而言，熟習此項技術者應了解，出於簡潔之目的，通常使用基於參考相關多肽之規範編號系統來表示多肽中之殘基，以使得胺基酸「對應於」位置190處之殘基，例如，實際上無需為特定胺基酸鏈中之第190 個胺基酸，而是對應於在參考多肽之190處發現之殘基；熟習此項技術者易於了解鑑別「相應」胺基酸之方法。舉例而言，熟習此項技術者將意識到可用於例如鑑別本文之多肽及/或核酸中之「相應」殘基之多種序列比對策略，包括軟體程式，例如BLAST、CS-BLAST、 CUSASW++、DIAMOND、FASTA、GGSEARCH/GLSEARCH、Genoogle、HMMER、HHpred/HHsearch、IDF、Infernal、KLAST、USEARCH、parasail、PSI-BLAST、PSI-Search、ScalaBLAST、Sequilab、SAM、SSEARCH、SWAPHI、SWAPHI-LS、SWIMM或SWIPE。
經設計 ：如本文所用之術語「經設計」係指以下劑：(i)其結構係由人手選擇；(ii)藉由需要人手之製程產生；及/或(iii)不同於天然物質及其他已知劑。
可偵測實體 ： 如本文所用之術語「可偵測實體」係指可偵測之任一元素、分子、官能基、化合物、片段或部分。在一些實施例中，可偵測實體係單獨提供或使用。在一些實施例中，可偵測實體係與另一劑締合(例如連結)來提供及/或使用。可偵測實體之實例包括(但不限於)：各種配位體、放射性核種(例如 3H、14C、18F、19F、32P、35S、135I、125I、123I、64Cu、187Re、111In、90Y、 99mTc、177Lu、89Zr等)、螢光染料(關於具體例示性螢光染料參見下文)、化學發光劑(例如吖啶酯、穩定的雙氧烷及諸如此類)、生物發光劑、可光譜拆分的無機螢光半導體奈米晶體(即量子點)、金屬奈米粒子(例如金、銀、銅、鉑等)奈米簇、順磁金屬離子、酶(關於酶之具體實例參見下文)、比色標記(例如染料、膠體金及諸如此類)、生物素、地高辛(dioxigenin)、半抗原及抗血清或單株抗體可用之蛋白質。
測定 ：本文所述之許多方法包括「測定」步驟。熟習此項技術者在閱讀本說明書時應了解，該「測定」可利用熟習此項技術者可獲得之多種技術中之任一者或經由使用熟習此項技術者可獲得之多種技術中之任一者來完成，包括例如本文明確提及之特定技術。在一些實施例中，測定涉及物理樣品之操縱。在一些實施例中，測定涉及資料或資訊之考慮及/或操縱，例如利用適於執行相關分析之電腦或其他處理單元。在一些實施例中，測定涉及自來源接收相關資訊及/或材料。在一些實施例中，測定涉及比較樣品或實體與相當參考物之一或多個特徵。
表現 ：如本文所用核酸序列之「表現」係指以下事件中之一或多者：(1)自DNA序列產生RNA模板(例如藉由轉錄)；(2)處理RNA轉錄物(例如藉由剪接、編輯、5’帽形成及/或3’端形成)；(3)RNA轉譯成多肽或蛋白質；及/或(4)多肽或蛋白質之轉譯後修飾。
凝膠 ：如本文所用之術語「凝膠」係指流變性質將其與溶液、固體等區分開之黏彈性材料。在一些實施例中，若組成物之儲存模數(G’)大於其模數(G’’)，則將該組成物視為凝膠。在一些實施例中，若在溶液中存在與黏性溶液中之纏絡分子不同之化學或物理交聯網絡，則將組成物視為凝膠。
同源性 ：如本文所用之術語「同源性」係指聚合物分子之間、例如多肽分子之間之總體相關性。在一些實施例中，若聚合物分子(例如抗體)之序列係至少80%、85%、90%、95%或99%一致，則該等聚合物分子視為彼此「同源」。 在一些實施例中，若聚合物分子之序列係至少80%、85%、90%、95%或99%相似，則將該等聚合物分子視為彼此「同源」。
一致性 ：如本文所用之術語「一致性」係指聚合物分子之間、例如核酸分子(例如DNA分子及/或RNA分子)之間及/或多肽分子之間之總體相關性。在一些實施例中，若聚合物分子之序列係至少25%、30%、35%、40%、45%、50%、55%、60%、65%、70%、75%、80%、85%、90%、95%或99%一致，則將該等聚合物分子視為彼此「實質上一致」。 例如，兩條核酸或多肽序列之一致性%之計算可藉由出於最佳比較之目的比對兩條序列來實施(例如，可在第一及第二序列中之一或兩者中引入空位用於最佳比對，且可出於比較之目的忽視非一致序列)。在某些實施例中，經比對用於比較目的之序列之長度係參考序列長度之至少30%、至少40%、至少50%、至少60%、至少70%、至少80%、至少90%、至少95%或實質上100%。然後比較相應位置之核苷酸。當第一序列中之位置由與第二序列中之相應位置相同之殘基(例如核苷酸或胺基酸)佔據時，則分子在該位置係一致的。兩條序列之間之一致性%係序列所共享之一致位置數之函數，考慮到為達成兩條序列之最佳比對而需要引入之空位數及每一空位之長度。兩條序列之間之序列比較及一致性%之測定可使用數學演算法來完成。舉例而言，兩條核苷酸序列之間之一致性%可使用Meyers及Miller之算法(CABIOS，1989，4: 11-17)來測定，該算法已納入ALIGN程式(2.0版)中。在一些例示性實施例中，使用ALIGN程式進行之核酸序列比較利用PAM120權重殘基表、空位長度罰分12及空位罰分4。 兩條核苷酸序列之間之一致性%可替代地使用GCG軟體包中之GAP程式使用NWSgapdna.CMP矩陣來測定。
活體外 ：如本文所用之術語「活體外」係指發生在人造環境中(例如在測試管或反應容器中、細胞培養物中等)而非多細胞生物體內之事件。
經分離 ：如本文所用，係指物質及/或實體已(1)與在最初產生時與其締合之至少一些組分分離(無論在自然界中及/或在實驗環境中)，及/或(2)藉由人手設計、產生、製備及/或製造。經分離之物質及/或實體可與約10%、約20%、約30%、約40%、約50%、約60%、約70%、約80%、約90%、約91%、約92%、約93%、約94%、約95%、約96%、約97%、約98%、約99%或大於約99%之最初與其締合之其他組分分離。在一些實施例中，經分離劑之純度係約80%、約85%、約90%、約91%、約92%、約93%、約94%、約95%、約96%、約97%、約98%、約99%或大於約99%。如本文所用，若物質實質上不含其他組分，則該物質係「純的」。在一些實施例中，如熟習此項技術者應理解，物質在與某些其他組分(例如一或多種載劑或賦形劑，例如緩衝劑、溶劑、水等)組合後仍可視為「經分離」或甚至「純的」；在此類實施例中，物質之分離%或純度係在不包括此類載劑或賦形劑之情況下計算。儘給出一個實例，在一些實施例中，當a)由於其衍生起源或來源而不與在自然界中在天然狀態下伴隨其之一些或所有組分締合；b)其實質上不含與在自然界中產生其之物種相同之物種之其他多肽或核酸；c)由不具在自然界中產生其之物種之細胞或其他表現系統表現或以其他方式與不具在自然界中產生其之物種之細胞或其他表現系統之組分締合時，在自然界中存在之生物聚合物(例如多肽或多核苷酸)視為「經分離」。因此，例如，在一些實施例中，化學合成或在不同於在自然界中產生其之細胞系統中合成之多肽視為「經分離」多肽。替代地或另外，在一些實施例中，已經受一或多種純化技術之多肽可視為「經分離」多肽，其在一定程度上已與a)在自然界中與其締合；及/或b)在最初產生時與其締合之其他組分分離。
核酸 ：如本文所用，在其最廣泛含義中，係指納入或可納入寡核苷酸鏈中之任一化合物及/或物質。在一些實施例中，核酸係經由磷酸二酯鍵納入或可納入寡核苷酸鏈中之化合物及/或物質。如根據上下文將清楚，在一些實施例中，「核酸」係指個別核酸殘基(例如核苷酸及/或核苷)；在一些實施例中，「核酸」係指包含個別核酸殘基之寡核苷酸鏈。在一些實施例中，「核酸」係或包含RNA；在一些實施例中，「核酸」係或包含DNA。在一些實施例中，核酸係一或多種天然核酸殘基，包含一或多種天然核酸殘基，或由一或多種天然核酸殘基組成。在一些實施例中，核酸係一或多種核酸類似物，包含一或多種核酸類似物，或由一或多種核酸類似物組成。在一些實施例中，核酸類似物與核酸之不同之處在於其不使用磷酸二酯骨架。舉例而言，在一些實施例中，核酸係在此項技術中已知且在骨架中具有肽鍵而非磷酸二酯鍵、視為在本發明之範圍內之一或多種「肽核酸」，包含該一或多種肽核酸，或由該一或多種肽核酸組成。替代地或另外，在一些實施例中，核酸具有一或多個硫代磷酸酯鍵及/或5'-N-亞磷醯胺鍵而非磷酸二酯鍵。在一些實施例中，核酸係一或多種天然核苷(例如腺苷、胸苷、鳥苷、胞苷、尿苷、去氧腺苷、去氧胸苷、去氧鳥苷及去氧胞苷)，包含該一或多種天然核苷，或由該一或多種天然核苷組成。在一些實施例中，核酸係一或多種核苷類似物(例如2-胺基腺苷、2-硫胸苷、次黃嘌呤、吡咯并-嘧啶、3-甲基腺苷、5-甲基胞苷、C-5丙炔基-胞苷、C-5丙炔基-尿苷、2-胺基腺苷、C5-溴尿苷、C5-氟尿苷、C5-碘尿苷、C5-丙炔基-尿苷、C5 -丙炔基-胞苷、C5-甲基胞苷、2-胺基腺苷、7-去氮腺苷、7-去氮鳥苷、8-側氧基腺苷、8-側氧基鳥苷、0(6)-甲基鳥嘌呤、2-硫胞苷、甲基化鹼基、嵌入鹼基及其組合)，包含該一或多種核苷類似物，或由該一或多種核苷類似物组成。在一些實施例中，核酸包含一或多种與天然核酸中之糖相比經修飾之糖(例如2'-氟核糖、核糖、2'-去氧核糖、阿拉伯糖及己糖)。在一些實施例中，核酸具有編碼功能基因產物(例如RNA或蛋白質)之核苷酸序列。在一些實施例中，核酸包括一或多個內含子。在一些實施例中，核酸係藉由以下中之一或多者來製備：自天然來源分離、基於互補模板藉由聚合之酶合成(活體內或活體外)、在重組細胞或系統中複製及化學合成。在一些實施例中，核酸之長度為至少3個、4個、5個、6個、7個、8個、9個、10個、15個、20個、25個、30個、35個、40個、45個、50個、55個、60個、65個、70個、75個、80個、85個、90個、95個、100個、1 10個、120個、130個、140個、150個、160個、170個、180個、190個、20個、225個、250個、275個、300個、325個、350個、375個、400個、425個、450個、475個、500個、600個、700個、800個、900個、1000個、1500個、2000個、2500個、3000個、3500個、4000個、4500個、5000個或更多個殘基。在一些實施例中，核酸部分或完全地係單股；在一些實施例中，核酸部分或完全地係雙股。在一些實施例中，核酸具有包含至少一個元件之核苷酸序列，該至少一個元件編碼多肽或係編碼多肽之序列之補體。在一些實施例中，核酸具有酶活性。
多肽 ：如本文所用係指胺基酸之任何聚合物鏈。在一些實施例中，多肽具有在自然界中存在之胺基酸序列。在一些實施例中，多肽具有不在自然界中存在之胺基酸序列。在一些實施例中，多肽具有經由人手之動作設計及/或產生來改造之胺基酸序列。在一些實施例中，多肽可包含天然胺基酸、非天然胺基酸或二者，或由其組成在一些實施例中，多肽可僅包含天然胺基酸或僅包含非天然胺基酸，或僅由其組成。在一些實施例中，多肽可包含D-胺基酸、L-胺基酸或二者。在一些實施例中，多肽可僅包含D-胺基酸。在一些實施例中，多肽可僅包含L-胺基酸。在一些實施例中，多肽可在多肽之N-末端、在多肽之C-末端或其任一組合處包括一或多個側基或其他修飾，例如修飾或連接至一或多個胺基酸側鏈。在一些實施例中，此類側基或修飾可選自由以下組成之群組：乙醯化、醯胺化、脂化、甲基化、聚乙二醇化等，包括其組合。在一些實施例中，多肽可為環狀，及/或可包含環狀部分。在一些實施例中，多肽不為環狀及/或不包含任一環狀部分。在一些實施例中，多肽為線性。在一些實施例中，多肽可為或包含釘形多肽。在一些實施例中，術語「多肽」可附加至參考多肽、活性或結構之名稱；在此類情況下，其在本文中用於指共享相關活性或結構且因此可視為相同之多肽類別或家族之成員的多肽。對於每一該類別，本說明書提供及/或熟習此項技術者將意識到已知胺基酸序列及/或功能之類別內之例示性多肽；在一些實施例中，此類例示性多肽係多肽類別或家族之參考多肽。在一些實施例中，多肽類別或家族之成員顯示與該類別之參考多肽；在一些實施例中與該類別內之所有多肽之顯著序列同源性或一致性，與其共享常見序列基元(例如特徵性序列元件)，及/或共享常見活性(在一些實施例中以相當水準或在指定範圍內)。舉例而言，在一些實施例中，成員多肽顯示與參考多肽至少約30%-40%、且通常大於約50%、60%、70%、80%、90%、91%、92%、93%、94%、95%、96%、97%、98%、99%或更大之總體序列同源性或一致性程度，及/或包括至少一個顯示極高序列一致性、通常大於90%或甚至95%、96%、97%、98%或99%之區域(例如在一些實施例中可為或包含特徵性序列元件之保守區)。此一保守區通常涵蓋至少3-4個且通常高達20個或更多個胺基酸；在一些實施例中，保守區涵蓋至少2個、3個、4個、5個、6個、7個、8個、9個、10個、11個、12個、13個、14個、15個或更多個鄰接胺基酸之至少一段。在一些實施例中，相關多肽可包含母體多肽之片段或由其組成。在一些實施例中，有用之多肽可包含複數個片段或由其組成，該等片段中之每一者係以相對於彼此不同於在所關注多肽中發現之空間排列(例如在母體中直接連接之片段可在所關注多肽中在空間上分開或反之亦然，及/或片段可以不同於母體之順序存在於所關注多肽中)發現於同一母體多肽中，以使得所關注多肽係其母體多肽之衍生物。
蛋白質 ：如本文所用之術語「蛋白質」係指多肽(即一串藉由肽鍵彼此連接之至少兩個胺基酸)。蛋白質可包括除胺基酸外之部分(例如，可為糖蛋白、蛋白聚糖等)及/或可以其他方式處理或修飾。熟習此項技術者應了解，「蛋白質」可為由細胞(具或不具信號序列)產生原樣之完整多肽鏈，或可為其特徵性部分。熟習此項技術者應了解，蛋白質有時可包括例如藉由一或多個二硫鍵連接或藉由其他方式締合之一個以上之多肽鏈。多肽可含有L-胺基酸、D-胺基酸或二者且可含有此項技術中已知之多種胺基酸修飾或類似物中之任一者。 有用的修飾包括 例如末端乙醯化、醯胺化、甲基化等。 在一些實施例中，蛋白質可包含天然胺基酸、非天然胺基酸、合成胺基酸及其組合。術語「肽」通常用於指長度小於約100個胺基酸、小於約50個胺基酸、小於20個胺基酸或小於10個胺基酸之多肽。在一些實施例中，蛋白質係抗體、抗體片段、其生物活性部分及/或其特徵性部分。
參考 ：如本文所用闡述相對於其實施比較之標準或對照。舉例而言，在一些實施例中，將所關注劑、動物、個體、群體、樣品、序列或值與參考或對照劑、動物、個體、群體、樣品、序列或值進行比較。在一些實施例中，參考或對照實質上係與所關注測試或測定同時測試及/或測定。在一些實施例中，參考或對照係歷史參考或對照，視情況地體現於有形介質中。通常，如熟習此項技術者應理解，參考或對照係在與評價下之條件或環境相當之條件或環境下測定或表徵。熟習此項技術者應了解，何時存在足夠相似性以證明依賴於特定可能的參考或對照及/或與特定可能的參考或對照比較係合理的。
樣品 ：如本文所用之術語「樣品」通常係指自所關注來源獲得或衍生而來之材料之等分試樣，如本文所述。在一些實施例中，所關注來源係生物或環境來源。在一些實施例中，所關注來源可為或包含細胞、組織或生物體，例如微生物、植物或動物(例如人類)。在一些實施例中，所關注來源係或包含生物組織或流體。在一些實施例中，生物組織或流體可為或包含羊水、房水、腹水、膽汁、骨髓、血液、母乳、腦脊液、耳垢、乳糜、食糜、射出物、內淋巴、滲出物、糞便、胃酸、胃液、淋巴、黏液、心包液、外淋巴、腹膜液、肋膜液、膿、稀黏液、唾液、皮脂、精液、血清、包皮垢、痰、滑液、汗液、淚液、尿液、陰道分泌物、玻璃狀液、嘔吐物及/或其組合或組分。在一些實施例中，生物流體可為或包含細胞內流體、細胞外流體、血管內流體(血漿)、間隙流體、淋巴液及/或透細胞流體。在一些實施例中，生物流體可為或包含植物滲出物。在一些實施例中，生物組織或樣品可例如藉由抽吸物、生檢(例如細針或組織生檢)、拭子(例如口、鼻、皮膚或陰道拭子)、刮取、手術、洗滌或灌洗(例如支氣管肺泡、導管、鼻、眼、口、子宮、陰道或其他洗滌或灌洗)來獲得。在一些實施例中，生物樣品係或包含自個體獲得之細胞。在一些實施例中，樣品係藉由任何適當方式直接自所關注來源獲得之「初級樣品」。在一些實施例中，如根據上下文應清楚，術語「樣品」係指藉由處理初級樣品(例如藉由去除初級樣品之一或多種組分及/或藉由向初級樣品中添加一或多種劑)獲得之製劑。舉例而言，使用半透膜過濾。此一「經處理樣品」可包含例如自樣品提取或藉由使初級樣品經受一或多種技術(例如核酸之擴增或反轉錄、某些組分之分離及/或純化等)獲得之核酸或蛋白質。
特異性（ specific ） ： 熟習此項技術者應理解，術語「特異性」在本文中用於具有活性之劑時意指該劑區分出潛在靶實體或狀態。舉例而言，在一些實施例中，若劑在一或多種競爭性替代靶存在下優先與其靶結合，則稱該劑「特異性」結合至該靶。在許多實施例中，特異性相互作用取決於靶實體之特定結構特徵(例如抗原決定基、裂縫、結合位點)之存在。應理解，特異性無需係絕對的。在一些實施例中，特異性可相對於一或多種其他潛在靶實體(例如競爭者)之結合劑之特異性來評估。在一些實施例中，特異性係相對於參考特異性結合劑之特異性來評估。在一些實施例中，特異性係相對於參考非特異性結合劑之特異性來評估。在一些實施例中，劑或實體在與其靶實體結合之條件下不可偵測地結合至競爭性替代靶。在一些實施例中，與競爭性替代靶相比，結合劑以較高之締合速率、較低之解離速率、增加的親和力、減少的解離及/或增加的穩定性結合至其靶實體。
特異性（ specificity ） ：如此項技術中已知，「特異性」係特定配位體區分其結合伴侶與其他潛在結合伴侶之能力之量度。
個體 ：如本文所用之術語「個體」係指生物體，例如哺乳動物(例如人類、非人類哺乳動物、非人類靈長類動物、靈長類動物、實驗室動物、小鼠、大鼠、倉鼠、沙鼠、貓、狗)。在一些實施例中，人類個體係成人、青少年或小兒個體。在一些實施例中，個體患有疾病、病症或病狀，例如可如本文所提供治療之疾病、病症或病狀，例如本文所列之癌症或腫瘤。在一些實施例中，個體易患疾病、病症或病狀；在一些實施例中，易患病個體易于患上疾病、病症或病狀及/或顯示增加的患上該疾病、病症或病狀之風險(與在參考個體或群體中觀察到之平均風險相比)。在一些實施例中，個體展示疾病、病症或病狀之一或多種症狀。在一些實施例中，個體不展示疾病、病症或病狀之特定症狀(例如 疾病之臨床表現) 或特徵。在一些實施例中，個體不展示疾病、病症或病狀之任何症狀或特徵。在一些實施例中，個體係患者。在一些實施例中，個體(subject)係投與及/或已投與診斷及/或療法之個體(individual)。
患有 ：「患有」疾病、病症及/或病狀之個體展示疾病、病症及/或病狀之一或多種症狀及/或已經診斷患有疾病、病症或病狀。
易患 ：「易患」疾病、病症及/或病狀之個體係患上疾病、病症及/或病狀之風險高於公眾成員之個體。在一些實施例中，易患疾病、病症及/或病狀之個體可能未經診斷患有疾病、病症及/或病狀。在一些實施例中，易患疾病、病症及/或病狀之個體可展現疾病、病症及/或病狀之症狀。在一些實施例中，易患疾病、病症及/或病狀之個體可能不展現疾病、病症及/或病狀之症狀。在一些實施例中，易患疾病、病症及/或病狀之個體將患上疾病、病症及/或病狀。在一些實施例中，易患疾病、病症及/或病狀之個體將不患上疾病、病症及/或病狀。
SARS-CoV-2
在一些實施例中，本文提供用於偵測及/或診斷SARS-CoV-2之組成物及方法。SARS-CoV-2係COVID-19之病原體。根據美國疾病控制中心(United States Centers for Disease Control，「CDC」)，COVID-19之早期症狀通常包括以下中之一或多者：發熱/風寒、咳嗽、呼吸短促或呼吸困難、疲勞、肌肉或身體疼痛、頭痛、味覺或嗅覺之新喪失、咽喉炎、充血或流鼻涕、惡心或嘔吐及/或腹瀉。更嚴重症狀通常包括例如呼吸困難、胸部持續疼痛或壓力、新擾亂、無法入睡或失眠及/或唇或臉發青。替代地或另外，COVID-19患者可展示低血液氧合(例如低於98%)及/或急性呼吸窘迫症候群(ARDS)及/或肺炎之一或多種症狀或特徵。
報導表明，60歲以上之個體及/或患有潛在免疫病狀之個體可在暴露於且感染SARS-CoV-2後具有尤高之患上COVID-19之風險。
SARS-CoV-2係冠狀病毒家族中之病毒。冠狀病毒家族之成員係具有有義單股RNA基因體之脂膜病毒。
已對多個人類樣品之SARS-CoV-2基因體進行測序；此類序列通常例如可經由公開及/或存放在公共可訪問資料庫中來獲得。
參見 NCBI 參考序列： NC_045512.2 ；重度急性呼吸症候群冠狀病毒 2 資料中心 (www.ncbi.nlm.nih.gov/labs/virus/vssi/#/virus?SeqType_s=Nucleotide&VirusLineage_ss=SARS-CoV-2,%20taxid:2697049) ； www.ncbi.nlm.nih.gov/genbank/sars-cov-2-seqs/#reference-genome 。
圖1呈現包括中之SARS-CoV基因體及開放閱讀框之表示。如可見，基因體係編碼由病毒編碼之蛋白酶所處理之串聯蛋白。
CRISPR/Cas 附帶活性（ Collateral Activity ）
最近，已鑑別出某些CRISPR/Cas酶，其在藉由結合至與其複合之嚮導RNA所識別之目標位點活化時具有非特異性裂解附帶核酸之能力。已顯示Cas12酶、Cas13酶及Cas14酶之代表性實例具有該附帶裂解活性。參見 例如Swarts及Jinek Mol Cell. 2019年2月7日;73(3):589-600.e4；Harrington L.B.等人，Science. 2018;362:839-842；Li S.Y.等人，Cell Res. 2018;28:491-493；Chen J. S.等人，Science. 2018;360:436-439；Abudayyeh O.O.等人，Science. 2016;353aaf5573；East-Seletsky A等人，Nature. 2016;538:270-273；Gootenberg JS等人，Science 2017;356:438-442；Myhrvold C等人，Science 2018;360:444-448；Gootenberg JS等人，Science 2018;360:439-444。一些CRISPR/Cas酶附帶裂解活性會消化或裂解單股核酸。一些CRISPR/Cas酶附帶裂解活性會消化或裂解雙股核酸。一些CRISPR/Cas酶附帶裂解活性會消化或裂解RNA。一些CRISPR/Cas酶附帶裂解活性會消化或裂解DNA。一些CRISPR/Cas酶附帶裂解活性會消化或裂解RNA及DNA二者。
此附帶活性已用於開發在生物及/或環境樣品中達成含有相關目標位點之核酸(或其補體)之偵測之偵測(例如 診斷)技術。參見 例如Gootenberg JS等人，Science 2017;356:438-442；WO2019/011022；US10494664B2；US10337051B2；US10266887；sherlock.bio/better-faster-affordable-diagnostic-testing。
本文提供偵測生物及/或環境樣品中之SARS-CoV-2之尤其有效之技術，包括藉由提供有效的該偵測之實例。舉例而言，本文例示使用某(些) Cas13酶偵測自鼻咽拭子分離之核酸中之SARS-CoV-2。
本文闡述一起達成重要且驚人的SARS-CoV-2偵測靈敏度及/或特異性之特定試劑，例如 目標位點、嚮導RNA序列、擴增及/或信號生成技術及其組合。本文亦闡述例如在偵測SARS-CoV-2方面驚人地有效之樣品、格式及各種條件(例如溫度、時間、組分濃度等)。
本文亦鑑別出某些問題之來源及/或提供允許該達成之關鍵見解。
所提供偵測技術
圖2提供如本文所例示之偵測分析之工作流程概述。圖2中所繪示之分析包括以下步驟：
(i) 樣品收集
(ii) 目標分離/擴增
(iii) CRISPR/Cas附帶活性
本文提供與該等步驟中之每一者相關之見解及/或技術。在一些實施例中，本文所述之多個步驟可同時實施。在一些實施例中，本文所述之一或多個步驟可在單一容器(例如一鍋式反應)中實施。在一些實施例中，擴增及CRISPR/Cas附帶活性可在單一容器中進行。
在一些實施例中，用於分離/擴增步驟中之具體分離技術係產生核酸之任一樣品處理。熟習此項技術者意識到引起穩定核酸分離之許多樣品處理技術。
圖2中所繪示之具體目標分離/擴增技術涉及環介導之等溫擴增(LAMP)。在一些實施例中，擴增步驟包括反轉錄LAMP (RT-LAMP)。熟習此項技術者將意識到某些所報導CRISPR/Cas附帶活性偵測方法(參見例如使用替代性擴增技術，例如基於核酸序列之擴增(NASBA)；股置換擴增；重組酶聚合酶擴增(RPA)；滾環式擴增(RCA))。在一些實施例中，此類替代性擴增技術中之一或多者可用於本發明實踐中(例如 與本文所述之其他態樣及/或特徵一起)。然而，在某些實施例中，本文鑑別出，LAMP可為較佳的，此至少係由於其提供增加的速度及特異性且在單一恆定溫度下操作。
熟習此項技術者將意識到，某些軟體包已經特異性開發以與LAMP技術一起使用，包括預測預期可用於任一給定目標核酸之引子之序列。本文尤其鑑別出使用此類預測之問題之來源，且驚人地發現相對於藉由此類預測生成之其他序列展示意外效用之特定序列。
在一些實施例中，擴增步驟包括包含啟動子序列之引子。在一些實施例中，引子包含RNA聚合酶啟動子序列。在一些實施例中，RNA聚合酶啟動子序列允許DNA在CRISPR/Cas酶偵測之前轉錄成RNA。在一些實施例中，RNA聚合酶啟動子包含pol I、pol II、pol III、T7、T3、SP6、U6、H1、反轉錄病毒勞斯肉瘤病毒(RSV) LTR啟動子、巨細胞病毒(CMV)啟動子、SV40啟動子、二氫葉酸還原酶啟動子、.β.-肌動蛋白啟動子、磷酸甘油激酶(PGK)啟動子及EF1.α.啟動子。
圖2中所繪示之具體CRIPR/Cas附帶活性分析使用Cas13酶。熟習此項技術者將意識到可用於本文所述分析之多種Cas13酶。另外，熟習此項技術者將意識到用於嚮導多核苷酸設計之多種方法、算法及軟體。參見例如sgRNA設計器(Broad)、CRISPR靶向基因設計器(Horizon Discovery)、https://en.wikipedia.org/wiki/CRISPR/Cas_Tools。
下文部分更詳細地論述所提供技術之某些態樣之多個特徵及/或實施例。
CRISPR/Cas 酶
在一些實施例中，本文之方法及組成物使用CRISPR/Cas酶。在一些實施例中，本文之方法及組成物使用V型或VI型CRISPR/Cas酶。在一些實施例中，本文之方法及組成物使用Cas12、Cas13及/或Cas14 CRISPR/Cas酶。在一些實施例中，本文之方法及組成物使用WO2016/166340；WO2016/205711；WO/2016/205749；WO2016/205764；WO2017/070605；WO/2017/106657中所述之CRISPR/Cas酶。在一些實施例中，本文之方法及組成物使用Cas13a CRISPR/Cas酶。在一些實施例中，本文之方法及組成物使用LwaCas13a CRISPR/Cas酶。
在一些實施例中，本文之方法及組成物使用熱穩定CRISPR/Cas酶。在一些實施例中，本文之方法及組成物使用由表1中所列之序列編碼之熱穩定CRISPR/Cas酶。
表1
| SEQ ID NO. | Cas酶 | 推定的序列 |
| 1 | TccCas13a (犬熱梭菌(Thermoclostridium caenicola)) | MKITKRKWGEHHPPLYFYRDEDSGRLLAQNDRKQDYTDTLFNDIAQD TFERSLRNRLLKTPEKGDKRFYSNEIVKLVEKLCQGADVAEIMKSMERN EKLRPKNEKEIKNLKKQLDGTLSEYGKRYTAPEGAMTLNDALFYLVEGN PLKQAMAKAELGKIREALIKEKENRINRVRYSIKNNKIPLRIQEDGGITPN NDRAAWLLGLMKPADPAKGITDCYPLLGELEEVFDFDKLSKTLHEKISR CQGRPRSIAMAVDEALKQYLRELWEKSPSRQQDLKYYFQAVQEYFKD NFPIRTKRMGARLRQELLKDKTSLSRLLEPKHMANAVRRRLINQSTQM HILYGKLYAYCCGEDGRLLVNSETLQRIQVHEAVKKQAMTAVLWSISRL RYFYQFEDGDILSNKNPIKDFRDKFLRDTNKYTHEDVEACKEKLQDFFPL KELQEKIKEDAKGLQETDNKQADTTDFKAIGHIVRDDRKLCNQLLAECV SCIGELRHHIFHYKNVTLIQALKRIADKVKPEDLSVLRAIYLLDRRNLKKAF AKRISSMNLPLYYREDLLSRIFKKEGTAFFLYSAKIQMTPSFQRVYERGK NLRREFECERMKAEASNGQNGQDGDRLKWFRQLAAGDSADTHFNW AVEAYAESAADVENNVEFDTDVDAQRALRNLLLLIYRHHFLPEVQKDET LVTGKIHKVLERNRQLSEGQGPNQGKAHGYSVIEELYHEGMPLSDLMK QLQRRISETERESRELAQEKTDYAQRFILDIFAEAFNDFLEAHYGEEYLEI MSPRKDAEAAKKWVKESKTVDLKTSIDEKEPEGHLLVLYPVLRLLDEREL GELQQQMIRYRTSLASWQGESNFSEEIRIAGQIEELTELVKLTEPEPQFA EEVWGKRAKEAFEDFIEGNMKNYEAFYLQSDNNTPVYRRNMSRLLRS GLMGVYQKVLASHKQALKRDYLLWSEKHWNVKDENGADISSAEQAQ CLLQRLHRKYAESPSRFTEEDCKLYEKVLRRLEDYNQAVKNLSFSSLYEIC VLNLEILSRWVGFVQDWERDMYFLLLAWVRQGKLDGIKEEDVRDIFSE GNIIRNLVDTLKGENMNAFESVYFPENKGSKYLGVRNDVAHLDLMRK NGWRLEAGKTCSVMEDYINRLRFLLSYDQKRMNAVTKTLQQIFDRHK VKIRFTVEKGGMLKIEDVTADKIVHLKGSRLSGIEIPSHGERFIDTLKALM VYPRG* |
| 2 | ThpCas13a (深海海旋菌(Thalassospira profundimaris)) | MRIIKPYGRSHVEGVATEQPRRKLRLNTRPDISRDIPGFAQSHDALIIAQ WISAIDKIATKPKPDQKPTQRQMNLRTTLGDAAWQHLMAKNLLPAAK DPAIREKLHLIWQSKIAPWGASRPQEEKRGKPTPKGGWYERFCGALSP EAITQNVARQIAKDIYDHLYVAAKRKGREPVKQGESSNKPGKFKPDRKL SLIEERAESIAKNALRPGTHAPCPWGQDDQAIYEQAGDVATKIYDDAR DYLEDKKRRSGNRNTSSVQYLPRDLAVKILYAQYGRVFGPDTTIKAALD EQQSLFALHTAIKDCYHRLVNDARKRHILRILPRNMAALFRLVRAQYDN RDINALIRLGKVIHYHAGEQGKDEHHGIRDYWPSQQDIQNSRFWGSD GQADIKRHEAFSRIWRHIIALASRTLHDWADPDSQKFTGDDDDILMRA GAIESNVWDAGRYERKCDVLFGAQASLFCGAEDFEKATLKQAITGTGN LRNATFHFKGKARFENELQRLADDVPVDVQSAIAALWQKDAEGRTRQ IAETLQAVLAGHFLSERQNRHILATLMAAMAQPGDVPLPRLRRVLARH DSICQRGRILPLPPCPDRAKLEESPALTCQYTVLKMLYDGPFRAWLAQQ NSTILNHYIDSTIARTNKAAQDMNGRKLAPAEKDLITARAADIPRLSVDE KMVDFLGRLTAATATEMRVQRGYQSDGEKAQKQAGYIGEFECDVIAR AFSDFLGQSGFDFVLKLKADTPKPDAAQCDVAALIAPGDVPALTPQAW QQVLYFILHLVPVDDASRLLHQTRKWQALEKKGKDKEVKKEKDKEVKK EDEKPDIADLQSVLMLYLDMHDAKFTGGAALHGIEKFAEFFVEKADFR AVFPPQSLQDQDRSIPRRGLREIVRFGHLPLLQHMSGTVKITHDNVVA WQTARTPDATGTSPIARRQKQREELHALAVERPARFRNADLHNYMHA LVDVIKHRQLSAQVTLSDQVRLHRLMMGVLGRLVDYAGLWERDLYFV LLALLYHHGVTPDDVLKGQGKRKLADGQVVEALKPKNRKAAAPVGVF DDLDHYGIYQDDRQSIRNGLSHFNMLRGGTAPDLSHWVNQTRRLVA HDRKLKNAVAKSVIEMLAREGFDLDWTIEPDSGKHILRHGKIRTRQAQ HFQKSRIRIEKKSAKPDKNDTVKIRENLHGDAMVERVARLFAARAQKYR DITTEKRLDHLFLKPKG* |
| 3 | AacCas12b (酸土脂環酸芽孢桿菌(Alicyclobacillus acidoterrestris)) | MAVKSIKVKLRLDDMPEIRAGLWKLHKEVNAGVRYYTEWLSLLRQENL YRRSPNGDGEQECDKTAEECKAELLERLRARQVENGHRGPAGSDDELL QLARQLYELLVPQAIGAKGDAQQIARKFLSPLADKDAVGGLGIAKAGN KPRWVRMREAGEPGWEEEKEKAETRKSADRTADVLRALADFGLKPL MRVYTDSEMSSVEWKPLRKGQAVRTWDRDMFQQAIERMMSWES WNQRVGQEYAKLVEQKNRFEQKNFVGQEHLVHLVNQLQQDMKEAS PGLESKEQTAHYVTGRALRGSDKVFEKWGKLAPDAPFDLYDAEIKNVQ RRNTRRFGSHDLFAKLAEPEYQALWREDASFLTRYAVYNSILRKLNHAK MFATFTLPDATAHPIWTRFDKLGGNLHQYTFLFNEFGERRHAIRFHKLL KVENGVAREVDDVTVPISMSEQLDNLLPRDPNEPIALYFRDYGAEQHF TGEFGGAKIQCRRDQLAHMHRRRGARDVYLNVSVRVQSQSEARGER RPPYAAVFRLVGDNHRAFVHFDKLSDYLAEHPDDGKLGSEGLLSGLRV MSVDLGLRTSASISVFRVARKDELKPNSKGRVPFFFPIKGNDNLVAVHE RSQLLKLPGETESKDLRAIREERQRTLRQLRTQLAYLRLLVRCGSEDVGR RERSWAKLIEQPVDAANHMTPDWREAFENELQKLKSLHGICSDKEW MDAVYESVRRVWRHMGKQVRDWRKDVRSGERPKIRGYAKDVVGGN SIEQIEYLERQYKFLKSWSFFGKVSGQVIRAEKGSRFAITLREHIDHAKED RLKKLADRIIMEALGYVYALDERGKGKWVAKYPPCQLILLEELSEYQFNN DRPPSENNQLMQWSHRGVFQELINQAQVHDLLVGTMYAAFSSRFDA RTGAPGIRCRRVPARCTQEHNPEPFPWWLNKFVVEHTLDACPLRADD LIPTGEGEIFVSPFSAEEGDFHQIHADLNAAQNLQQRLWSDFDISQIRL RCDWGEVDGELVLIPRLTGKRTADSYSNKVFYTNTGVTYYERERGKKRR KVFAQEKLSEEEAELLVEADEAREKSVVLMRDPSGIINRGNWTRQKEF WSMVNQRIEGYLVKQIRSRVPLQDSACENTGDI |
| 4 | AkCas12b (酸土 脂環酸芽孢桿菌(Alicyclobacillus kakegawensis)) | MAVKSIKVKLRLSECPDILAGMWQLHRATNAGVRYYTEWVSLMRQEI LYSRGPDGGQQCYMTAEDCQRELLRRLRNRQLHNGRQDQPGTDADL LAISRRLYEILVLQSIGKRGDAQQIASSFLSPLVDPNSKGGRGEAKSGRKP AWQKMRDQGDPRWVAAREKYEQRKAVDPSKEILNSLDALGLRPLFAV FTETYRSGVDWKPLGKSQGVRTWDRDMFQQALERLMSWESWNRR VGEEYARLFQQKMKFEQEHFAEQSHLVKLARALEADMRAASQGFEAK RGTAHQITRRALRGADRVFEIWKSIPEEALFSQYDEVIRQVQAEKRRDF GSHDLFAKLAEPKYQPLWRADETFLTRYALYNGVLRDLEKARQFATFTL PDACVNPIWTRFESSQGSNLHKYEFLFDHLGPGRHAVRFQRLLVVESE GAKERDSVVVPVAPSGQLDKLVLREEEKSSVALHLHDTARPDGFMAE WAGAKLQYERSTLARKARRDKQGMRSWRRQPSMLMSAAQMLEDA KQAGDVYLNISVRVKSPSEVRGQRRPPYAALFRIDDKQRRVTVNYNKLS AYLEEHPDKQIPGAPGLLSGLRVMSVDLGLRTSASISVFRVAKKEEVEAL GDGRPPHYYPIHGTDDLVAVHERSHLIQMPGETETKQLRKLREERQAV LRPLFAQLALLRLLVRCGAADERIRTRSWQRLTKQGREFTKRLTPSWRE ALELELTRLEAYCGRVPDDEWSRIVDRTVIALWRRMGKQVRDWRKQV KSGAKVKVKGYQLDVVGGNSLAQIDYLEQQYKFLRRWSFFARASGLVV RADRESHFAVALRQHIENAKRDRLKKLADRILMEALGYVYEASGPREG QWTAQHPPCQLIILEELSAYRFSDDRPPSENSKLMAWGHRGILEELVN QAQVHDVLVGTVYAAFSSRFDARTGAPGVRCRRVPARFVGATVDDSL PLWLTEFLDKHRLDKNLLRPDDVIPTGEGEFLVSPCGEEAARVRQVHA DINAAQNLQRRLWQNFDITELRLRCDVKMGGEGTVLVPRVNNARAK QLFGKKVLVSQDGVTFFERSQTGGKPHSEKQTDLTDKELELIAEADEAR AKSVVLFRDPSGHIGKGHWIRQREFWSLVKQRIESHTAERIRVRGVGSS LD |
| 5 | BhCas12b (外村尚芽孢桿菌(Bacillus hisashii)) | MATRSFILKIEPNEEVKKGLWKTHEVLNHGIAYYMNILKLIRQEAIYEHH EQDPKNPKKVSKAEIQAELWDFVLKMQKCNSFTHEVDKDEVFNILREL YEELVPSSVEKKGEANQLSNKFLYPLVDPNSQSGKGTASSGRKPRWYN LKIAGDPSWEEEKKKWEEDKKKDPLAKILGKLAEYGLIPLFIPYTDSNEPI VKEIKWMEKSRNQSVRRLDKDMFIQALERFLSWESWNLKVKEEYEKV EKEYKTLEERIKEDIQALKALEQYEKERQEQLLRDTLNTNEYRLSKRGLRG WREIIQKWLKMDENEPSEKYLEVFKDYQRKHPREAGDYSVYEFLSKKE NHFIWRNHPEYPYLYATFCEIDKKKKDAKQQATFTLADPINHPLWVRFE ERSGSNLNKYRILTEQLHTEKLKKKLTVQLDRLIYPTESGGWEEKGKVDI VLLPSRQFYNQIFLDIEEKGKHAFTYKDESIKFPLKGTLGGARVQFDRDH LRRYPHKVESGNVGRIYFNMTVNIEPTESPVSKSLKIHRDDFPKVVNFKP KELTEWIKDSKGKKLKSGIESLEIGLRVMSIDLGQRQAAAASIFEVVDQK PDIEGKLFFPIKGTELYAVHRASFNIKLPGETLVKSREVLRKAREDNLKLM NQKLNFLRNVLHFQQFEDITEREKRVTKWISRQENSDVPLVYQDELIQI RELMYKPYKDWVAFLKQLHKRLEVEIGKEVKHWRKSLSDGRKGLYGISL KNIDEIDRTRKFLLRWSLRPTEPGEVRRLEPGQRFAIDQLNHLNALKED RLKKMANTIIMHALGYCYDVRKKKWQAKNPACQIILFEDLSNYNPYEER SRFENSKLMKWSRREIPRQVALQGEIYGLQVGEVGAQFSSRFHAKTGS PGIRCSVVTKEKLQDNRFFKNLQREGRLTLDKIAVLKEGDLYPDKGGEK FISLSKDRKCVTTHADINAAQNLQKRFWTRTHGFYKVYCKAYQVDGQT VYIPESKDQKQKIIEEFGEGYFILKDGVYEWVNAGKLKIKKGSSKQSSSEL VDSDILKDSFDLASELKGEKLMLYRDPSGNVFPSDKWMAAGVFFGKLE RILISKLTNQYSISTIEDDSSKQSM |
| 6 | LsCas12b (沈積物萊西氏菌(Laceyella sediminis)) | MSIRSFKLKIKTKSGVNAEELRRGLWRTHQLINDGIAYYMNWLVLLRQE DLFIRNEETNEIEKRSKEEIQGELLERVHKQQQRNQWSGEVDDQTLLQ TLRHLYEEIVPSVIGKSGNASLKARFFLGPLVDPNNKTTKDVSKSGPTPK WKKMKDAGDPNWVQEYEKYMAERQTLVRLEEMGLIPLFPMYTDEV GDIHWLPQASGYTRTWDRDMFQQAIERLLSWESWNRRVRERRAQFE KKTHDFASRFSESDVQWMNKLREYEAQQEKSLEENAFAPNEPYALTKK ALRGWERVYHSWMRLDSAASEEAYWQEVATCQTAMRGEFGDPAIY QFLAQKENHDIWRGYPERVIDFAELNHLQRELRRAKEDATFTLPDSVD HPLWVRYEAPGGTNIHGYDLVQDTKRNLTLILDKFILPDENGSWHEVK KVPFSLAKSKQFHRQVWLQEEQKQKKREVVFYDYSTNLPHLGTLAGAK LQWDRNFLNKRTQQQIEETGEIGKVFFNISVDVRPAVEVKNGRLQNGL GKALTVLTHPDGTKIVTGWKAEQLEKWVGESGRVSSLGLDSLSEGLRV MSIDLGQRTSATVSVFEITKEAPDNPYKFFYQLEGTELFAVHQRSFLLAL PGENPPQKIKQMREIRWKERNRIKQQVDQLSAILRLHKKVNEDERIQAI DKLLQKVASWQLNEEIATAWNQALSQLYSKAKENDLQWNQAIKNAH HQLEPVVGKQISLWRKDLSTGRQGIAGLSLWSIEELEATKKLLTRWSKR SREPGVVKRIERFETFAKQIQHHINQVKENRLKQLANLIVMTALGYKYD QEQKKWIEVYPACQVVLFENLRSYRFSYERSRRENKKLMEWSHRSIPKL VQMQGELFGLQVADVYAAYSSRYHGRTGAPGIRCHALTEADLRNETNI IHELIEAGFIKEEHRPYLQQGDLVPWSGGELFATLQKPYDNPRILTLHAD INAAQNIQKRFWHPSMWFRVNCESVMEGEIVTYVPKNKTVHKKQGK TFRFVKVEGSDVYEWAKWSKNRNKNTFSSITERKPPSSMILFRDPSGTF FKEQEWVEQKTFWGKVQSMIQAYMKKTIVQRMEE |
| 7 | Cas12 | MFKKKLFDDEEFISLAQNQEESNALNAFKGFTTHFKDFQENRKNMYS EDKESTAIAYRIIHENLPVFITNNIRFEKIINELDRSNIHSIEKELKEELANN KLKDIFNIEYFQNTLTQNDITRYNTIIGGKVKADGKKVQGLNEYINLFN QHNKDKKLPLLKPLYKQILSEENSASFIVPAFEKDNEVLQSIFDFWNKCI IDAKGPISGKKYNLLSKIQSLLQNLDKLKNNQLEEMYFENENLSTISNDV YGQWNLIRDALGNFYNSIDAKKNKKDYYSWKEIQDALVYYKQTNDEY KDIDQKAFLIYFKEMKVNDGEENTNNNIINLINERYKRIEPLLKEDRDN RKDLHQDKGKVAIIKEFLDSLKLLQNTIKLLYVDDSLDNMNYDFYNQLT DYYETLRPLNTLYNRVRNYMTRKPFSEEKFVLTFNSPTLLDGWDLNKE EANLGVILRKDNKYYLGIMNKGDNKIFKKYDEEPGDDYYEKMVYKLLP GPNRMLRKVFFSNKNIEYYKPNQDIQNLYNKGEFKKGESLNKESLHKLI DFYKNSISKNGDWSVFNFKFKKTTAYDDISQFYKDVENQGYKLFFKTIK TSYIDQLVNEGKLYLFQIYNKDFSENKKRKDESNPNLHTIYFKNLFSEDN LKNVVYKLNGKAEVFYRKKSIEYPEEIRRKGHHYNELKDKFDYPIIKDKR YSEDKFLFHVPITLNFLAKSDEKVNEMVKNYIAATNEKIHIIGIDRGERN LLYLSLIDSNGNIVKQQSLNIIELPKYQKQIDYHAKLNEKEKQRLAARQN WDVIENIKELKEGYLSQVIHQIARLMVDYKAILVMEDLNFGFKRGRFK VEKQVYQKFEKMLIDKLSYLVFKEKNLCEPGGSLRAYQLSAPFKSFKAL GKQSGMIFYVPAQYTSKIDPTTGFYNFLNIDVSNLARSKETFSKFDKIVY NKKEDYFEFYCKMINFESANQLTKKSQNKANAELKEFQWILCSTHHDR FKVERKNNQINYCKINVNEELKKLLNSKGINYEKSNDLKSEILNIDESKFF KELGYLLKILVSLRYNNGKKGSEEQDFILSPVKNASGKFFCTLDNNNTLP LDADANGAYNIALKGLMIVQRVKAGGKLDLSISKDDWINFLIMNKKLP K |
| 8 | Cas12 | MSNQSVFKDFTNLYELSKTLRFELKPVGKTLRMLEDAKVFKTDELIQKK YEQTKPFINKLHQEFVKESLEGRSLEGLESYQDILKEWQKDKKDKIAQK NLGIKEKELYKQVTQLFNAKAKEWSEPYAHLGLKKKDIGILFEEGVFKIL KEKYNNDKDAKITNKVTGEIFFEDFWKGFVGYFQKFFETRKNFYKDDG TSTAIATRIVAQNLKRFCDNIGLFEKIKDQIDSSEVEQSFGISMEKVFSLD FYNQCLLQGGIDKYNEILGGKTLENGEKFKGINELINKYRQDNKGDKSS FLKILDKQILSEKESFIDEIKNDKELEETLKNLHETAKVKTKIFGTLFEDFIG NNTKYDLAKIYISKEAFNTISHKWTGGTDLFAENLFNALKDEQILKSSAK KKDGSYVFPDFIEFLHIKTALENVPKDINFWKERYYVNKEGENKEFFLG NGEIWQQFLQIFNFEFNELFQKEIIDNQTGKKMHIGYKVYKEEISKLLE DFKVDKDSTVIIKHFADSVLWIYQMAKYFALEKKRTWRDEYDLDTFYT DPKNGYLAFYENAYEEIVQIYNKLRNYLTKKPYSTEKWKLNFQNSTLAS GWDKNKEADNFTVILRKDGKYFLGLMRKGANKLFDKRYGSEFSQGLE KGKYEKMNYKYFPSPSKMIPKTSTQVHEVKKHFKNSSEPFFLEESSSLG KFIKQLKITKEVFDLNNFEYKKSYLSTLNGESPDESQRVKADSKKTGQV KLFQKEFLNLSQNELLYKKSLFAWVDFCKEYLDCFPSTGDGFLQFKKYIQDTEKYESIDQFYKDIERGGYKISFQNISEEYISCKNQNSELYLFKIHNKD WNLKDGKPKTGMKNLHTMYFESLFSSENIAQNFPMKLNGQAEIFYRP KTDINKLEMKKDSKGKNVVDHKRYEEDKIFFHLPMTLNRGKSLFNFNV QLNNFLADNPEINIIGVDRGEKHLAYYSVINQNQEILDGGTLNVVKGG NGKDIDYHKKLEDKAEKREQARKDWQDVEGIKDLKKGYISQVVRKLA DLAIEHNAIIVFEDLNMRFKQIRGGIEKSVYQQLEKALIEKLSFLVRKNE KNPEEAGYLLKAYQLSAPFETFQRIGKQTGIIFYTQASYTSKIDPLTGWR PNLYLKYSNAKKAKADISKFSEIEFINNRFEFTYDLQEFRSQKDKKKEYP KKTLWTLCSSVERYRWNRKLNDNKGGYEHYSDLTSDFKKLFKKYNINI NEDILGQIENMDTDDRKNNARFFSGFMFFWNLICQIRNTNSDVISGE SDNDFILSPVEPFFDSRKASQFGSDLPENGDDNGAFNIARKGIMILKKI SQYVEENENCDKLKWGDLYISHTDWDNFI |
| 9 | Cas12 | MTNYTDFIGLYPVQKTLRFELRPQGKTAEKMRESGLLEQDREKAKNYI VMKALIDDYHRRFINELLEKASFDWQPLFEALNNVKVNKDDKSKKELE KEQLHMRKELIGLFEKDERFKYLFSEKLFSELLNKEISERNDPDEMEAM RSFDRFSGYFIGFHENRRNIYSNEDKHNSLAYRVVAENFPKFADNCRK YSLIKENMQEAVVEFKKEIASVVDIDVDQMFDISYFNKVLTQKGIDDY NTMLGGVSEEGSVKIRGLNEFLNLYYQKVTDNKRIKMAPLYKQILCESK TKSFIPYMFENDEEVISSINQYYDSVKYDILQRSVYLLSNYKEYDASKIFI DQKSISSISIVLFGSWETLGGLMQIYKADQIGDPGLEKTRKKVDKWLSS SYFTLKEVFEAIGEQDPFRVYVEKLSLVLKNIEEFDKSCLLEGTHFSGDEL LTQDIKGFLDLLMEVQHLMKPFNAKEDLDKDAAFYSEYNEIYEALSEIIP LYNKVRNYATKKKYSTYKIKMNFGNPTLAAGWDLNKERDNTAVILLR GNNYYLGIMNPKKKTKFEELPSGEDNDCYRKMVYKLLPGPNKMLPKV FFSKKGIGTFNPSKEILEGYETGKHKLGDSFDIDYCHSLIDFFKENIPKYG DWGTYEFKFSPTEEYSDISQFYKEVSEQGYKITFQNISRKAIDDLVNNG ALFLYQIYNKDFSEHSKGKNNLHTMYWKAAFSEENLRNVVIKINGEAE LFYRDKSDISKTEHSAGTILVNRTDRKDNPIPNSIYYELFKYKTGQIKSVS DEAKQYLDDLVTHEAKYPITKDRRYTEDRMFFHIPITLNFGSSGNTNIN KAVIDHVLNSKDVHIIGIDRGERNLLYVSVIDRKGNIIKQRSLNVIDGIDY HEKLDQREKENISARKSWSNVEKIKDLKEGYLSYVIH |
| 10 | Cas12 | MKDFYQFTNLYALSKTLRFSLIPTPATKQMLEDAKVFEKDETIQKKYEA TKPYFDRLHREFALEALQDQKLDFKNYLELYRKYKADKKASGKLLINIEK DLRKEVVKLFDKQGEKWAKQYPGLKNKNIGVLFKEAVFTVILKERYGN EKETQILDESSGQLVSIFDSWKGFIGYFKKFHETRKNFYKDDGTSTALA TRIIDQNLKRFCDNILIFESTKEKVDFSEVEISFGKPLSEVFTLEFYNTCFL QNGIDFYTKILGGETLQNGEKVKGLNECINLHKQKTGEKLPFFKSLDKQ ILSEKDKFFIDEISNETQLLEVLKSFVASAESKTDTIKTLVDDFVKDQDKY DLNYIYFSNDGLNTITRKWTTETQVFEEALYTALKAAKVVSSSAKKNEG GYSFPDFIPFAHLKTALESIKIDGTIWRDNFNAIENFEEKSIWAQFLAIY NFELSNLFETEIKNPEIGNCPTIGYNVYKQDFEELLKSFVYDPNAKVTIK NFADNVLSIYQMAKYFAVEKKRGWNTDYELDVFYTDPQNGYLQYYE NAYEEIVQVYNKLRNYLTKKPYSEEKWKLNFDSGTPIKYTTRAIIFNNTT NERYYLGLLKKGVAKPREFEPINNNIISSGEFRRMIIQQLKFQTLAGKGY VRDFGVKYSEDKDGVKHLQQLIKKQYLSKYPCLKKIADGVYNDKKAFD ADIKDVLLETYNLDFQPISEEFILNKNRLGEIYLFEIHNKDWNLKDGKNK SGSKNLHTMYFESLFVDKTTFKLNNEGAEVFYRPATNEGKLGTKKDRN GKIIINHKRYATDKILFHCPIGLNKDAGKSYTFNAKINNMLANNPDINII GVDRGEKHLAYYSVITQKGKILDRGSLNKVEGGDKQEIDYAKKLEETAK NREQARKDWQAVEGIKDLKRGYISQVVRKLADLAIEHNAIIVFEDLNM RFKQIRGGIEKSVYQQLEKALIDKLSFLVMKGEADPEKAGHLLKAYQLV APFESFQSMGKQTGIIFYTQANYTSKIDPITGWRPNLYLKYTSAEKAKA DILKFSKIEFVNNRFELTYDIKNFVLDKKVVLSNKTKWTVCSSVERFRW NRRLESNQGNYEHYENLTENLSSLFKDFGFEIEQNIIRQVEQLATKGNE QFFRSFIFYVNLIFQIRNTDAKAKDQNKEDFILSPVEPFFDSRTPEKFGE NLPENGDDNGAFNIARKGIIMLNKISAYKQEVGNVDKIIWKDLFISAAE WDNFTQE |
| 11 | Cas12 | MDSYEQFTKLYPIQKTIRFELKPQGRTKEHFDNSNFLEKDRERDDNYKI LKEVIDDYHREFIDECLSNIQLNWDDLKKFSEEYRRSKEKKNNRDSESE QKRMSTTSETRAINKKNLEAEQKRMRGEIVSAFKKDDRFKHLFSEKLFS ILLKNQIYEKGTLEEIEAFDCFNKFSGYFKSFHENRKNMYSDEDKETAIS YRIINENFPKLLDNFEKYQYVCREYPEQIREAESTLAEAGCYIKMDEIFSI DNFNNVMMQGGKESGISRYNLAIGGIVQGTGEKPKGLNEFLNLAYQ NEPNGRKKIRMEPLYKQILSKEESFSYRLEAFTDDSQLLSAIRSFFDIVEK DKNGNIFDRAVNLMSSFSNYDTSKIYIRKAYLNQVSKEIFGYRGKSDSK PAKTADESLNKSGGWEKLGQMLRDYKADSIGDRNLEKTCKKVDKWL DSDEFTLSDILGAISLAGSNETFEAYVSEICVARRNIDKEKEKEKNINVEK ISGDTESIQIIKALLDSVQEFFHLLSPFQLHPNTPHDWTFYAEFNDIYDK LSAITPLYNQARNHLTKKNLDTSKIKLNFNNPTLANGWDVNKEYENTA VILIRDGKYYLGIMNPKNKRKIKFDEGSGAGPFYQKMVYKLLPGPYRM LPKVFFAKKNIDYYNPSQEIREGYKAGKHKKGKEFDKGFCHKLIDFFKES IQKNENWKVFDFKFSPTESYDDISEFYQEVEKQGYRMYFVNIPSDTIDR YVEGGDMFLFQIYNKDFAKGAKGNKDMHTLYWNAVFSEENLQKGV MKLSGEAELFYRKKSDIKDPPHREGEILVNRTYIDRTHVSGVMGEQNT VKESRIPVPDEIHKNLFDYYNHGRELTKEEKEYCDKVGSFKAYYGIVKD RRYLENKMYFHVPLTLNFKAIGEKRINKMAIEKFLTDENACIIGIDRGER NLLYYSIIDRNGKIIDQKSLNVIDGFDYHEKLSQRQTEREVARQSWNSI GKIKDLKEGYLAKAVHEISKMAIKYNAIVVLEDLHFGFKKGRLKVEKQIY QKFEEMLINKLNYLVFKDVSDSSDAGGVLNAYQLTAPLESFSKLGKQS GILFYVPAAFTSVIDPTTGFVDLFNSSSITSTQKKKEFLQRFESIVYSARD GGIFAFTFDYRNFSKIATDHRNMWTVYTHGERIRYVRDEKCYKTTDPT KRIKEALSGIEYDDGSDIRDKITQSGDNNLINTVYHSFMDTIKMRNKD GRIDYIISPVKNRNGEFFRSDYKHRDFPVDADANGAYHIALKGELLMR MIGKTYDSNSDKMPKLEHKDWFEFMQTRGDQ |
| 12 | Cas12 | MKKEKEFKSFGDFTNLYEISKTLRFELKPVENTQTMLDEADVFGKDKVI KDKYTKTKPFIDKLHREFVDESLKDVSLSGLKKYSEVLENWKKNKKDKD IVKELKKEEERLRKEVVEFFDNTAKKWANEKYKELGLKKKDIGILFEESV FDLLKEKYGEEQDSFLKEEKGDFLKNEKGEKVSIFDEWKGFVGYFTKFQ ETRKNFYKNDGTETALATRIIDQNLKRFCDNIDDFKKIKNKIDFSEVEKN FNKTADVFSLDFYNQCLLQKGIDSYNEFIGGKTLENGKKLKGVNELVNEYRQKNKNEKVSFLKLLDKQILSEKEKLSFGIENDEQLLVVLNSFYETAE EKTKILRTLFGDFVEHNENYDLDKTYISKVAFNTISHKWTNETHKFEELL YGAMKEDKPIGLNYDKKEDSYKFPDFIALGYLKKCLNNLDCDTKFWKE KYYENNADKKDKDKGFLTGGQNAWDQFLQIFIFEFNQLFNSEAFDNK GKEIKIGYDNFRKDFEEIINQKDFKNDENLKIAIKNFADSVLWIYQMAK YFAIEKKRGWDDDFELSEFYTNPSNGYSLFYDRAYEEIVQKYNDLRNYL TKKPYKEDKWKLNFENPTLANGFDKNKESDNSTVILRKKRKYYLGLMK KGNNKIFEDRNKAEFIRNIESGAYEKMAYKYLPDVAKMIPKCSTQLNE AKNHFRNSADDLEIKKSFSNPLKITKRIFDLNNIQYDKTNVSKKISGDNK GIKIFQKEYYKISGDFDVYKSALNDWIDFCKDFLSKYDSTKDFDFSILRKT KDYKSLDEFYVDVAKITYKISFTPVSESYIDQKNKNGELYLFEIYNQDFAK GKMGAKNLHTLYFENVFSPENISKNFPIKLNGNAELFFRPKSIESKKEKR NFVREIVNKKRYSEDKIFFHCPITLNRETGSIYRFNNYVNNFLSENNINII GVDRGEKHLAYYSVIDKNGVKIGGGSFNEINKVDYAKKLEERAGEREQ SRKDWQVVEGIKDLKKGYISQVVRELADLAIKHNAIIVLEDLNMRFKQI RGGIEKSIYQQLEKALIDKLSFLVEKGEKDPNQAGHILKAYQLAAPFTSF KDMGKQTGIVFYTQASYTSKTCPNCGFRKNNNKFYFENNIGKAQDAL KKLKTFEYDSENKCFGLSYCLSDFANKEEVEKNKNKKRNNAPYSDIEKK DCFELSTKDAVRYRWHDKNTERGKTFFEGESVYEEKEEKEIGQTKRGL VKEYDISKCLIGLFEKTGLDYKQNLLDKINSGKFDGTFYKNLFNYLNLLFE IRNSISGTEIDYISCPECQFHTDKSKTIKNGDDNGSYNIARKGMIILDKIK QFKKENGSLDKMGWGELFIDLEEWDKFAQKKNNNIIDK |
| 13 | Cas12 | MKSFDSFTNLYSLSKTLKFEMRPVGNTQKMLDNAGVFEKDKLIQKKY GKTKPYFDRLHREFIEEALTGVELIGLDENFRTLVDWQKDKKNNVAM KAYENSLQRLRTEIGKIFNLKAEDWVKNKYPILGLKNKNTDILFEEAVFG ILKARYGEEKDTFIEVEEIDKTGKSKINQISIFDSWKGFTGYFKKFFETRK NFYKNDGTSTAIATRIIDQNLKRFIDNLSIVESVRQKVDLAETEKSFSISL SQFFSIDFYNKCLLQDGIDYYNKIIGGETLKNGEKLIGLNELINQYRQNN KDQKIPFFKLLDKQILSEKILFLDEIKNDTELIEALSQFAKTAEEKTKIVKKL FADFVENNSKYDLAQIYISQEAFNTISNKWTSETETFAKYLFEAMKSGK LAKYEKKDNSYKFPDFIALSQMKSALLSISLEGHFWKEKYYKISKFQEKT NWEQFLAIFLYEFNSLFSDKINTKDGETKQVGYYLFAKDLHNLILSEQID IPKDSKVTIKDFADSVLTIYQMAKYFAVEKKRAWLAEYELDSFYTQPDT GYLQFYDNAYEDIVQVYNKLRNYLTKKPYSEEKWKLNFENSTLANGW DKNKESDNSAVILQKGGKYYLGLITKGHNKIFDDRFQEKFIVGIEGGKY EKIVYKFFPDQAKMFPKVCFSAKGLEFFRPSEEILRIYNNAEFKKGETYSI DSMQKLIDFYKDCLTKYEGWACYTFRHLKPTEEYQNNIGEFFRDVAED GYRIDFQGISDQYIHEKNEKGELHLFEIHNKDWNLDKARDGKSKTTQK NLHTLYFESLFSNDNVVQNFPIKLNGQAEIFYRPKTEKDKLESKKDKKG NKVIDHKRYSENKIFFHVPLTLNRTKNDSYRFNAQINNFLANNKDINII GVDRGEKHLVYYSVITQASDILESGSLNELNGVNYAEKLGKKAENREQ ARRDWQDVQGIKDLKKGYISQVVRKLADLAIKHNAIIILEDLNMRFKQ VRGGIEKSIYQQLEKALIDKLSFLVDKGEKNPEQAGHLLKAYQLSAPFET FQKMGKQTGIIFYTQASYTSKSDPVTGWRPHLYLKYFSAKKAKDDIAK FTKIEFVNDRFELTYDIKDFQQAKEYPNKTVWKVCSNVERFRWDKNL NQNKGGYTHYTNITENIQELFTKYGIDITKDLLTQISTIDEKQNTSFFRD FIFYFNLICQIRNTDDSEIAKKNGKDDFILSPVEPFFDSRKDNGNKLPEN GDDNGAYNIARKGIVILNKISQYSEKNENCEKMKWGDLYVSNIDWDN FVTQANARH |
| 14 | Cas12 | MNTQKKEFNPKSFKDFTNLYSLNKTLRFSLTPNKKTAEILEFNKQKEVK CFSNDRKIAGAYQEIKKYLNKLHQEFIQEAMKFFAFSEEELKGFEKEYLN LLNFTDKDNFKKKNKIRNEYEQERKILTIKIATYFSKFKSEKYQSFNLANI TGKKVFSILEQKYKEDKKTLKIIHIFKYKPTKDEKKEGEAVNFSTYLTGFN ENRKNFYKSEDKAGQFATRTIDNLAQFIKNKKLFEDKYQKNYSKIGILD EQIKIFNLDYFNNLFLQEGLDEYNGILGNNKGEENKSNEGINQKINIFK QKEKARLKKEKENFNKSDFPLFKELYKQIGSIRKENDVYVEIKTDKELVE ELNNFPKNVENYLKDIQSFYKTFFEKLQNEEYELDKIYLPKSVGTYFSYIA FSDWNKLAFIYNKRYKNEKIKIVEGGDVNVQYRSLEVLKNRIDELKDED NLNFNKFFIDKLKFNEAKKENNWQNFWFCIEYYINSQFIGGEKNILNKE KNEYEILPFGSLKELKEKYFEAVKKYKEKMVDTESGLTDDEEKEIKETLK NYLDRIKEIERIAKYFDLKKSFEEIKQEDLDSNFYGEYQKVVDKTNELKIY QYYSEFRNYLTQNNSVEEKIKLNFNSGLLLDGWDLNKEKVKFSIIFQEN GKYYLGIINKEKDKTILDKDKHPEIFTKNSDFRKMEYKLFPSPSKMLPKIS FSETAKKGDEDVGWSEEIQKIKDEFAEFQEYKKKSKDNWKDEFNRGK LNKLIDYYKQVLEKHSEGYMNTYNFELKDSSKYKNLGEFNDDIARQNY KVKFVGIDKNYIDEKVANGELFLFQIYNKDFSEDKKEGSTNNLETIYFKE LFSKENLENPVFKLSGGAEMFFRNKIEKKKEKKKLDKDGKPMISKKGEK VVDKRRFSENKILFHLPIEINYGKGKMPNFNKKINEYISKNPENIKIIGID RGEKHLLYYSIIDQNGNNIESMSLNAVDEFGNFVNPEKLEEYEIDNNG KKERRWKYIVNDKEIKVTNYQRKLDELEKERQKSRQSWQNINKIKNLK KGYISFVVKKIVDLAIENNAIIILEDLNFGFKSFRQKIEKNVYQQFEKALID KLGFVVDKQKQNQRFAPQLSAPFESFQKIGKQTGIVYYVLANNTSKVC PSCQWIKNFYLKYEKKNTIFNLQKNQKLKVFFEQEKNRFRFEYQMSKE YISVYSDVDRQRYDKTKNQNKGGYLEYKNSNQKEIIDKDGVIQKQSITL QLKELFKENHIDLEKEILKQLDNKKEKNSGYTGVYNKFIYLFNLILQIRNA ISFREKDYIQCPSCHFDTRKENYLKINDGDGNGAYNIALRGLYLLKGKN GIINNLEKIKLIFSNNDYFQWAKKLKNKK |
| 15 | Cas12 | MEEKMLKSYDYFTKLYSLQKTLRFELKPIGKTLEHIKNSGIIESDETLEEQ YAIVKNIIDKLHRKHIDEALSLVDFTKHLDTLKTFQELYLKRGKTDKEKEE LEKLSADLRKLIVSYLKGNVKEKTQHNLNPIKERFEILFGKELFTNEEFFLL AENEKEKKAIQAFKGFTTYFKGFQENRKNMYSEEGNSTSIAYRIINENL PLFIENIARFQKVMSTIEKTTIKKLEQNLKTELKKHNLPGIFTIEYFNNVLT QEGISRYNTIIGGKTTHEGVKIQGLNEIINLYNQQSKDVKLPILKPLHKQI LSEEYSTSFKIKAFENDNEVLKAIDTFWNEHIEKSIHPVTGNKFNILSKIE NLCDQLQKYKDKDLEKLFIERKNLSTVSHQVYGQWNIIRDALRMHLE MNNKNIKEKDIDKYLDNDAFSWKEIKDSIKIYKEHVEDAKELNENGIIK YFSAMSINEEDDEKEYSISLIKNINEKYNNVKSILQEDRTGKSDLHQDKE KVGIIKEFLDSLKQLQWFLRLLYVTVPLDEKDYEFYNELEVYYEALLPLNS LYNKVRNYMTRKPYSVEKFKLNFNSPTLLDGWDKNKETANLSIILRKN GKYYLGIMNKENNTIFEYYPGTKSNDYYEKMIYKLLPGPNKMLPKVFFS KKGLEYYNPPKEILNIYEKGEFKKDKSGNFKKESLHTLIDFYKEAIAKNED WEVFNFKFKNTKEYEDISQFYRDVEEQGYLITFEKVDANYVDKLVKEG KLYLFQIYNKDFSENKKSKGNPNLHTIYWKGLYDSENLKNVVYKLNGE AEVFYRKKSIDYPEEIYNHGHHKEELLGKFNYPIIKDRRYTQDKFLFHVPI TMNFISKEEKRVNQLACEYLSATKEDVHIIGIDRGERHLLYLSLIDKEGNI KKQLSLNTIKNENYDKEIDYRVKLDEKEKKRDEARKNWDVIENIKELKE GYMSQVIHIIAKMMVEEKAILIMEDLNIGFKRGRFKVEKQVYQKFEK MLIDKLNYLVFKNKNPLEPGGSLNAYQLTSKFDSFKKLGKQSGFIFYVP SAYTSKIDPTTGFYNFIQVDVPNLEKGKEFFSKFEKIIYNTKEDYFEFHCK YGKFVSEPKNKDNDRKTKESLTYYNAIKDTVWVVCSTNHERYKIVRNK AGYYESHPVDVTKNLKDIFSQANINYNEGKDIKPIIIESNNAKLLKSIAEQ LKLILAMRYNNGKHGDDEKDYILSPVKNKQGKFFCTLDGNQTLPINAD ANGAYNIALKGLLLIEKIKKQQGKIKDLYISNLEWFMFMMSR |
| 16 | Cas12 | MNNYDEFTKLYPIQKTIRFELKPQGRTMEHLETFNFFEEDRDRAEKYKI LKEAIDEYHKKFIDEHLTNMSLDWNSLKQISEKYYKSREEKDKKVFLSE QKRMRQEIVSEFKKDDRFKDLFSKKLFSELLKEEIYKKGNHQEIDALKSF DKFSGYFIGLHENRKNMYSDGDEITAISNRIVNENFPKFLDNLQKYQE ARKKYPEWIIKAESALVAHNIKMDEVFSLEYFNKVLNQEGIQRYNLALG GYVTKSGEKMMGLNDALNLAHQSEKSSKGRIHMTPLFKQILSEKESFS YIPDVFTEDSQLLPSIGGFFAQIENDKDGNIFDRALELISSYAEYDTERIYI RQADINRVSNVIFGEWGTLGGLMREYKADSINDINLERTCKKVDKWL DSKEFALSDVLEAIKRTGNNDAFNEYISKMRTAREKIDAARKEMKFISE KISGDEESIHIIKTLLDSVQQFLHFFNLFKARQDIPLDGAFYAEFDEVHSK LFAIVPLYNKVRNYLTKNNLNTKKIKLNFKNPTLANGWDQNKVYDYAS LIFLRDGNYYLGIINPKRKKNIKFEQGSGNGPFYRKMVYKQIPGPNKNL PRVFLTSTKGKKEYKPSKEIIEGYEADKHIRGDKFDLDFCHKLIDFFKESIE KHKDWSKFNFYFSPTESYGDISEFYLDVEKQGYRMHFENISAETIDEYV EKGDLFLFQIYNKDFVKAATGKKDMHTIYWNAAFSPENLQDVVVKLN GEAELFYRDKSDIKEIVHREGEILVNRTYNGRTPVPDKIHKKLTDYHNG RTKDLGEAKEYLDKVRYFKAHYDITKDRRYLNDKIYFHVPLTLNFKANG KKNLNKMVIEKFLSDEKAHIIGIDRGERNLLYYSIIDRSGKIIDQQSLNVI DGFDYREKLNQREIEMKDARQSWNAIGKIKDLKEGYLSKAVHEITKM AIQYNAIVVMEELNYGFKRGRFKVEKQIYQKFENMLIDKMNYLVFKD APDESPGGVLNAYQLTNPLESFAKLGKQTGILFYVPAAYTSKIDPTTGF VNLFNTSSKTNAQERKEFLQKFESISYSAKDGGIFAFAFDYRKFGTSKTD HKNVWTAYTNGERMRYIKEKKRNELFDPSKEIKEALTSSGIKYDGGQN ILPDILRSNNNGLIYTMYSSFIAAIQMRVYDGKEDYIISPIKNSKGEFFRT DPKRRELPIDADANGAYNIALRGELTMRAIAEKFDPDSEKMAKLELKH KDWFEFMQTRGD |
| 17 | Cas12 | MASSHFISLDNSFSKFTNLYSLSKTLRFELVPTENTTVMLENNNVFKKD QIIQVKYEKTKPFIDRLHREFIKEALSNYAVSGLQEYFEILRAGGKKANLD SAKKQLRKHVVDQFNATASLWVSRHKDVGFKGEGIELLFKEAVFKLLK EKYGTDMNALIEDNHGKQISIFDSWKGFTGYFDKFQQTRRNLYKDDG KEGRVATRIIDQNLTRFCDNIFVYEKIKDKVSFIDVEKSFGKTCSEVFIPD YYNTCLLQDGIDSYNEFIGGKPLENGEKVQGLNELINLYRQTTGDKVPY FKKLEKQILGEKDEVFIDEITDEDFVPRVLAFYRTVDAKYKLFLKLLDDFV TNQDVYELSQIYISKKGLQEKLYRWLTPSAREVYDEELFEVLKKAKKVN NKDKQKVSGYVPDFVEVLYIKQALENIDAKLIWSDRYYSDGENEGIIDK GFSSWKQFLVILNHEYRQLLSFEDHVIIDKELDFDKEVKQLTDTVEIVSQ DKNARTVTYRGGYDVYKAKLAELGQSFEKDTCTKKVIKNFADSVLSMY HFAMMFAVWDDTYPLDVFYTNNEFGYLLYYEDAYKNIVQEYNKLRN YLTKKPYSTEKWKLNFENPTLAAGFDKNKESDNSTVILRQGDKYFLGV MKKGFNKIFDNSQISQTGNSPEAYFEKMVYKYTKDVVTGIPKSSTQVK EVQEHFRNSDEDFFLEECSSVGNFIVPLKITKEIFDLNNKVYAKEDISQA MYRWALNTDEEKNYVKSFQKSYLSLGGSPELYCKSVTLWIGFCLNFLK SYPSAAYFDYSQLRQASDYESVDECYQELNNAGYTILFQNVSEKYVRV KNKNGELYLFQIKNKDWNEGSTGKKNLHTLYFESLFSKENAKQGFPFK LSGNAELFFRPGSIEQTYERRNFPREIPLKRRYSKDGIFFHIPVQVNRTK VGSPNQFNKEVNDFLAGNPNINIIGVDRGEKHLVYYSVISQNGEKIDG GSFNEINGQDYHDKLEKRAKEREQQRRDWETVEGIKDLKKGYISQVV KKLADLAIEHNAIIVMEDLNMRFKQIRGGIEKSVYQQLEKALIDKLSFLV NKGEVDPQKAGHLLKAYQLTAPIDAFKDMGKQTGIMFYTQAAYTSKI DPVTGWRPHLYLKYSSVEKAKDDISRFTKIAYKNDRFEFTYNITDFRTQ KEWPLKTEWTVCSCVERFRWNKKLANGKGDYEHYPNVTDDFKKLFD SVGINYLQENIKSQVVNLDENTNVEFFREFIKLFALVCQIRNTNSEEAG NLNDFILSPVEPFFDSRSAEDFGKGLPSNGDENGAYNIARKGMIILNTL STFKNDHGSCEGLSWGDLYISDTQWDDFAQSFHG |
| 18 | Cas12 | MDAKEFTGQYPLSKTLRFELRPIGRTWDNLEASGYLAEDRHRAECYPR AKELLDDNHRAFLNRVLPQIDMDWHPIAEAFCKVHKNPGNKELAQD YNLQLSKRRKEISAYLQDADGYKGLFAKPALDEAMKIAKENGNESDIE VLEAFNGFSVYFTGYHESRENIYSDEDMVSVAYRITEDNFPRFVSNALI FDKLNESHPDIISEVSGNLGVDDIGKYFDVSNYNNFLSQAGIDDYNHII GGHTTEDGLIQAFNVVLNLRHQKDPGFEKIQFKQLYKQILSVRTSKSYI PKQFDNSKEMVDCICDYVSKIEKSETVERALKLVRNISSFDLRGIFVNKK NLRILSNKLIGDWDAIETALMHSSSSENDKKSVYDSAEAFTLDDIFSSVK KFSDASAEDIGNRAEDICRVISETAPFINDLRAVDLDSLNDDGYEAAVS KIRESLEPYMDLFHELEIFSVGDEFPKCAAFYSELEEVSEQLIEIIPLFNKA RSFCTRKRYSTDKIKVNLKFPTLADGWDLNKERDNKAAILRKDGKYYLA ILDMKKDLSSIRTSDEDESSFEKMEYKLLPSPVKMLPKIFVKSKAAKEKY GLTDRMLECYDKGMHKSGSAFDLGFCHELIDYYKRCIAEYPGWDVFD FKFRETSDYGSMKEFNEDVAGAGYYMSLRKIPCSEVYRLLDEKSIYLFQI YNKDYSENAHGNKNMHTMYWEGLFSPQNLESPVFKLSGGAELFFRK SSIPNDAKTVHPKGSVLVPRNDVNGRRIPDSIYRELTRYFNRGDCRISD EAKSYLDKVKTKKADHDIVKDRRFTVDKMMFHVPIAMNFKAISKPNL NKKVIDGIIDDQDLKIIGIDRGERNLIYVTMVDRKGNILYQDSLNILNGY DYRKALDVREYDNKEARRNWTKVEGIRKMKEGYLSLAVSKLADMIIE NNAIIVMEDLNHGFKAGRSKIEKQVYQKFESMLINKLGYMVLKDKSID QSGGALHGYQLANHVTTLASVGKQCGVIFYIPAAFTSKIDPTTGFADLF ALSNVKNVASMREFFSKMKSVIYDKAEGKFAFTFDYLDYNVKSECGRT LWTVYTVGERFTYSRVNREYVRKVPTDIIYDALQKAGISVEGDLRDRIA ESDGDTLKSIFYAFKYALDMRVENREEDYIQSPVKNASGEFFCSKNAG KSLPQDSDANGAYNIALKGILQLRMLSEQYDPNAESIRLPLITNKAWLT FMQSGMKTWKN |
| 19 | Cas12 | MVNKQNERGDFDDLTNLYEISKTLRFELVPVGETDRMLKEENVFKVD ENIKRKYQQTKLFFDRIHREFAKEALSVEGILSELEEYLAIFIEWRKDKKI HEKTLNQKEKELRKQVVSAFNAMANKWIERYGDVNLKKKNVEFLFEE GIFRVLKERYGEEDGSTITASDTGEVFSIFDSWKGFTGYFAKFFETRKNF YKDDGTATAIATRIVDENLRRFCDNLIVAQRLTENIDFSEVENNFQIKIK EVLFMEFYNKCLLQDDIDFYNKVIGGETLKTGEKLKGINELVNLHRHKT GEKLPFLKTLDKQILGRKEQFLDEIESEEELLEKLKDFQNVATKKIKVIKSL FGDFVENNENYDLEKIYISKKAFNTISRKWTGETEQFEKLLFESMKSDK PAGLKYDKKENNYKFPDFIAVSYIKDALENFSGEQKFWKDRYYIELELD NQVVWKQFLDIFYWEFSSLFKRSFVNKETGEISEVGCDIFEKKFINLIDD FEYNQKSKILIKDFADSVLSVYQMANYFSLEKKRKWSTEFETDSKFYDD SEIGFRNCFYEDVFEGIVQVYNKLRNYLTKKPFSEEKWKLNFENPTLAA GWDKNKEKDNSTVILRKDEKYFLAIMKKGNNVIFDDRNKALFSQNLE HGKYEKVVYKFAKDVTLGIPKSTTQTKSVIAHFKNSDEDYQITNGSAVG DFLEPLVVTKRIFELNNKIYSKNNLGKVLYRSEVSKDKQKEYIKLFQKKYL VLGGNKNLYRDAVKEWIDFCKSFIKVYPSYKYFDFSLLKEAVEYNSVDE FYKELNSYGYAISFQDISCDYIEEKNKNGELYLFQIKNKDWNKGSTGMK NLHTLYFESLFSEENIKNNFVTKLNGGAEIFYRPKTSKEKLGRKKIVRNG QEVFVVNHKRYSEDKIFFHCSIALNRGKGKLLKFNARINDLLANNPDIN VIGVDRGEKHLAYYSIIDQKCKILDSGTLNEVGAKVDYHEKLSNRAKKR EDGRRDWGWGQIEDIKNLKKGYVSQVVHKLAELIIKYNAILVFEDLN MRFKQIRGGIEKSIYQQLEKALIDKLNFLVKKGEKDSKSAGHLLKAYQL AAPFETFDKMGKQTGVIFYTQASYTSKIDPITGWRPNLYLKHSNANDS QKKIAKFSRIEFINDRFEFEYDLKKFIEMKEVPENTKWTLCSCVQRYRW NRKLNANKGGYDSYNDLTKNFKALFESVGIDIKKNIKEQIVKMEIKGNE KFFKSFIFYWQLLCQIRNTDELKKGDDNDFILSPVEPFFDSRKKNGDDL PKNGDDNGAYNIARKGVIVLNKISEFSKQNGNCEKCGWKELYVSAKD WDDFVQAK |
| 20 | Cas12 | MQNKQSFADFTNLYSLSKTLRFELKPIGQTQAMLDENKIFEVDENRKK AYDKTKPYFDRLHREFINESLSNAQLKGISEYFETFKQFRSNQNNKDLK ELINKQQKFLRHQIVTLFDENGKHWATTKYAHLKIKKKNLDILFDEQVF YILKERYGSEKETQLVDKETGAVTSIFDNWKGFTGYFTKFFETRKNFYK SDGTSTALATRIIDQNLNRFFDNLETFHKIKDKIDVKEVEIFFKLKADNV FSIDFYNQCLLQNGIDKYNDFLGGQTLENGEKQKGINEIINKYRQDNK DQKLPFLKKLDKQILSEKDRFINEIESKEEFFQVLTEFYQSATVKVTIIKTL LNDFVHNTDKYKLEKIYLTKEAFNTIANKWTDETQIFEDNLDLVLKNKK ITAKQDFIPLAYIKEALEVIEKDRKFFKDRYYNDPQIGFFPDQSYWEQFL AILNFEFMTHFQRVAKDKITGKKIELGYFVFEKRIKELLDSDPSLNSQSKI IIKEFADEVLHIFQMAKYFALEKKREWKGDYYQLDDQFYNHIDYGFKD QFYENAYEKIVQPYNKIRNYLTKKPYSDVKWKLNFGNPTLANGWDKN KEADNTAVILKKDGNYYLGVMKKGKNKIFSDQNKEKYKAYNSAYYEKL VYKLFPDPSKMFPKVCFSKKGLNFFQPSEEILRIYKNNEFKKGNTFSISS MQKLIAFYIDCLGLYEGWKHYEFKNIKDVRQYKENIGEFYADVAESGY KLWFEKISEEYITQKNQLGELFLFQIYNKDFAKKTTGRKNLHTIYFEELFS QTNIDNNFPFKLNGQAELFYRPKSLEKIEEKRNFKRSIVNKKRYTQNKIF FHVPITLNRTSENIGRFNVRVNNFLANNSNVNIVGVDRGEKNLAYYSII KQNGEVLKSGSLNIINGVDYHALLTDRAQRREQERRNWQDVESIKDL KRGYISQVVHELVSLAIKYNAIIVMEDLNMRFKQIRGGIEKSTYQQLEK ALIEKLNFLVNKEETDSNQAGNLLNAYQLTAPFKTFKDMGKQTGIIFYT QASYTSKIDPLTGWRPNIYLRYSNAKQAKADILMFTNIYFSEKKDRFEF TYDLEKIDDKRKDLPIKTEWTVCSNVERFSWEKSLNNNKGGYVHYPIQ DSNGEESITSKLKKLFMDFGIDLTDIKTQIESLDTNKKDNANFFRKFIFYF QLICQIRNTQVNKSDDGNDFIFSPVEPFFDSRFADKFRKNLPKNGDEN GAYNIARKGLIILHKISDYFVKEGSTDKISWKDLSISQTEWDNFTTDK |
| 21 | Cas12 | MDKQKNKLQNFTNLYELSKTLRFELKPVGETQHLLEENKVFGIDGNIK KKYEATKPFFDRLHRKFVKEALVNIALGGLDNYLEVYKKFTNDRKDKEN QKELEKQEKLLRKQIKIFFDSQANQWKEKYNKINFKKSGLNILFEESIFQ LLKEIYGKEDDAFLKNDDNEFIFDKDGNKISIFDSWKGFTGYFKKFFETR KNFYKDDGTSTAIATRIIDQNLRRFCDNIFIYNKIKNKLDFSSLEKEQDV VLEEIFTTAYYMDCILQDDIDLYNGVLGGETLDDGTKIKGLNEIINKYRQ DNKGDKIPFFKKLDKQILSEKDRKFLDEIESEEELAELLKIFINNTEAKVKV FDELVNQLCVNDSDFELDKIYISKEAFNTISHKWTNQTHEFERVLFEEM KPDKITGLDYKKAEDKYKFPDFIALKYIIKSLNTLDKDSEFWKSHYYKTEE NQNAILSLEEKVGEQFLQIYKYELQRLHSRNVNVENKDGKMKEKEIGL DYSLTTVKELLKNFKLTDKSKIIIKDFADNVLQYYQLAKYFSVEKNREWN YTKLELADFYINPDFGYEIFYGNAYEEIIQIYNKLRNYLTKKPFSEEKWKL NFENPTLAGGWDKNKERGNATVILRKNEKYYLGIMAKGYNDIFTDKN KDKFDGEGYEKMVYKLFPGPNKMMPKVCFSKKGLDFFEPSEKIIDIYK DGKFKQGDTFSIDSMQQLIDFYKRALREYNGWKMYDFSKLKDTNDYT TNIGEFYNDVACAGYKVWFDNISEEYIQEKNENGELYLFEIHNKDWNL KDEKKKTGTKNLHTLYFESLFSDENALRDFVMKLSGEAELFFRPKTNAD KLGYRKDKKGNKVVKNKRYSEDKMFLHLSINLNRGKGQAFWFNRNIN NFLANNSDINVIGIDRGEKHLAYYSVISQQGEILDNGSLNEIAGVDYYA KLSKRAKEREGQRKDWQAVSDIKNLKKGYISQVVRKLADLAIEHNAIIV LEDLNMRFKQIRGGIEKSIYQQLEKALIEKLNFLVNKKEIDSDKAGNLLR AYQLTAPFETFQKMGKQTGIIFYTQASYTSKIDPLTGWRPNLYLKKGN AKINKEQIEKFSKIEFTNNRFEITYDLKNFGDKKKKYPQKTKWTLCSSVE RWRWDRKLNNNKGGYIHYEDLTTEFKSLFEKFEIDIEGDILEQIKTIDEN DRNNARLFSGFIYLWGLLSQIRNTDGELDEKIKKLEREDKNEEISEKEKF DVDFILSPVEPFFDSRTPEKFGENLPKNGDDNGAYNIARKGIITLERIKK FYELSDKEREKLKYPDLFITNAEWDDFATKRDS |
| 22 | Cas13 | MDNNTTLEKTELGLGITYNHDKVEDKHYFGGFFNLAQNNIDLVAQEF KKRLLVQGKDSINIFSNYFSDQCSITNLERGIKVLSEYFPVIFYFDLDENN KSKSIRQHIILLLDTINNLRNYYTHYYHKKVIIDDALYPLLDTILLKVVLEIK KKKLKEDKTKQLLKKGLEKEMAILFNLMKKEQKEKKIKGWNIDKNIKG AVLNRAFSHLLYNDGISDYRKSKSNTEDENLKDTLSESGILFLLSFFLNKK EQEQLKANIKGYKGKIASIPDEEITLKNNSLRNMATHWTYSHLTYKGLK HRIKTDHEKETLLVNMVDYLSKVPNEIYQNLSEQNKSLFLEDINEYMRDNEENNDSSEASRVIHPVIRKRYENKFAYFAIRFLDEFAEFPTLRFMVN VGNYIHDNRKKDIGGTSLITNRTIKQQINVFGNLTEIHKKKNDYFEKEE NKEKILEWELFPNPSYHFQKENIPIFIDLEKSKETNELAKEYAKEKKKIFG SSRKKQQNTAKKNREAIINLVFDKYKTSDRKTVTFEQPTALLSFNELNA FLYAFLVENKTGKELEKIIIEKIANQYQILKNCSSTVDKTNDSIPKSIKKIA HPTTDSFYSEGKKIDIEKLERDIKIEIEKTNEKLETIKENETSAKNYKRNER DIQKRKLYRKYVFFTNEIGIEATWITNDILRFLDNKENWKGYQHSELQK FISQYDNYKKEALGLLESEWNLESEAFFGQKLKRIFQSNFTFETFYKKYL DNRKDTLETYLSAIENLKTMTDVPPKILKKSWAELFRFFDKKIYLLSTIET KINELITKPINLSRGVFDEKPTFINGKSPNKENDQHLFANWFIHAKEQTI FQDFYNLALETPKEINNLKKQNYKLERSINNLKIEDIYIKQMVDFLYQKL FEQSFKGSLQDLYTSKEKREVEKSKAKNEQTPDESFIWKKQVEINALN GRIIAKTKIKDIGKFKNLLTDNKITHLISYDNRIWNFSLDNDGDTTKKLYS LNTELESYERIRREKLLKQIQEFEQFLLKQETEYSAERKHPEKFEKDGNP NFKKYIIEGMLNKITPVNEIEELEILKSKEDVFKIDFNEIVKLNNESIKKGY LLIMIRNKFAHNQLIDKNLFTFSLQLYSKNENENFSEYLDKVCQKIIQEFI EKLK |
| 23 | Cas13 | MNETDYLAKRLEYNYASIEDKHYFGGYFNLAQNNINDLSKAFKEKFG MKPKSCILDFFTQDKAIAEYQLGVEFLQKNLPVIRYLYLPTSHKRFENVP KNQLISEQRNYFKNSLKVLKNLIRDYRNFYTHHFHKPIPVFPETYKLLDD LFLAVANDVKKHRMKTDASKQLLKKGLIEELAQLEKLKLEDLKKLKREG KKVNLNDKEAITNAILNDSFSHLLPKENTISKYYSAVPTEDIDTENGVTIS ESGIIFLLGLFLTKKQSEDLRSRVKGFKAKLIVNPENPINKKNNSLKYMA THWVFGYLGFKGLKNRFTTTFTKDTLLAQIVDELSKVPDELYQVLPEEL KNEFLEDMNEYLKEENSESLDKATVIHPVIRKRYENKFAYFALRFLDEFV DFPTLRFQLHLGNYVHDKREKPIEGTKYVTERIVKEKIKAFAKLSEAAQL KQKYFEEKENHQSIGLQLYPNPSYNFVGNNIPIHLNLNEHFFPKEVKIV AGRLKKRNSSYKSDHPEEYKVRTDNKIKPDAILQDLGKPEKLAPVAML SLNELPALLHLVLTKKTPEEIEIIIAQKIAERYNVLTNYKAGDDISKGQITK NLLKAKQKKEVNLDKLQLAIEKEIAVTNDKLQTIALHIKERNDPKQKRK YVFTNKEIGLQVTWLANDLKRFMPKGSRQNWRGQHHSQLQKSLAFY DIQPKEPLSLLEEVWDFKNEAYLWNNGIRRSFDKRDFISFYTSYLNNRK ETFQRFKDQLNGIRSNKKILDKFIKQQHLWNLFHKRLYVIDTIEEQVEKL LVKPMQFPKGVFDHKPTYIKGKSIQENPECFADWYVAWNQHTDYQK FYSWDRDYKSAYLSGEQEKTEKRFIRVQGSKINKVKQQDVLLAKMASII FNELYLPEDAEHLDLNLSDIYKTQTERKAEIEAALIQSHKTTGDNSANIIK STSAWTLTVPYCSKNIYEPQVKLKELGKFKKFIASQKVQTLFEYKPQKI WNKTELEEVLELKANSYEVIRRDYLLKSIQEFEKYMIKKLPTLIDTNEHP NFNKYLTTFLKSLELVSEEDAKWLISKKDFDTTPIDELKKQSKIMEKAFLL VMIRNKFSHNQLPRKIYYDEIYKNVPNAVSINFNELFLEYTNQTILEFK |
| 24 | Cas13 | MESIIGLGLSFNPYKTADKHYFGSFLNLVENNLNAVFAEFKERISYKAK DENISSLIEKHFIDNMSIVDYEKKISILNGYLPIIDFLDDELENNLNTRVKN FKKNFIILAEAIEKLRDYYTHFYHDPITFEDNKEPLLELLDEVLLKTILDVK KKYLKTDKTKEILKDSLREEMDLLVIRKTDELREKKKTNPKIQHTDSSQIK NSIFNDAFQGLLYEDKGNNKKTQVSHRAKTRLNPKDIHKQEERDFEIPLSTSGLVFLMSLFLSKKEIEDFKSNIKGFKGKVVKDENHNSLKYMATHR VYSILAFKGLKYRIKTDTFSKETLMMQMIDELSKVPDCVYQNLSETKQK DFIEDWNEYFKDNEENTENLENSRVVHPVIRKRYEDKFNYFAIRFLDEF ANFKTLKFQVFMGYYIHDQRTKTIGTTNITTERTVKEKINVFGKLSKMD NLKKHFFSQLSDDENTDWEFFPNPSYNFLTQADNSPANNIPIYLELKN QQIIKEKDAIKAEVNQTQNRNPNKPSKRDLLNKILKTYEDFHQGDPTAI LSLNEIPALLHLFLVKPNNKTGQQIENIIRIKIEKQFKAINHPSKNNKGIP KSLFADTNVRVNAIKLKKDLEAELDMLNKKHIAFKENQKASSNYDKLLK EHQFTPKNKRPELRKYVFYKSEKGEEATWLANDIKRFMPKDFKTKWK GCQHSELQRKLAFYDRHTKQDIKELLSGCEFDHSLLDINAYFQKDNFE DFFSKYLENRIETLEGVLKKLHDFKNEPTPLKGVFKNCFKFLKRQNYVTE SPEIIKKRILAKPTFLPRGVFDERPTMKKGKNPLKDKNEFAEWFVEYLE NKDYQKFYNAEEYRMRDADFKKNAVIKKQKLKDFYTLQMVNYLLKEV FGKDEMNLQLSELFQTRQERLKLQGIAKKQMNKETGDSSENTRNQTY IWNKDVPVSFFNGKVTIDKVKLKNIGKYKRYERDERVKTFIGYEVDEK WMMYLPHNWKDRYSVKPINVIDLQIQEYEEIRSHELLKEIQNLEQYIY DHTTDKNILLQDGNPNFKMYVLNGLLIGIKQVNIPDFIVLKQNTNFDKI DFTGIASCSELEKKTIILIAIRNKFAHNQLPNKMIYDLANEFLKIEKNETY ANYYLKVLKKMISDLA |
| 25 | Cas13 | MEDKTTGAGISYDHTLMEDKHFFGGFLNLAQNNIDALLKAFKERFNV RYQSKQFAEVCFSDKLPDQDYLDRTLFLETHLPFIKYIGGKEANNRGTF RKNITLFFESIEQLRNFYTHYYHKPILFPEELYENLDRIFVEVSKEVKTHKV KNDQTRHLLTKNLANELDIRYKKNVEKLKELKAQGKKVNIHDKEAIKNS VLNNAFNHLIYKKEEDVFATEAYKSKYNLEDPSKNGISLSQSGLLFLLSM FLNKKDIEALKSRVKGFKAKIIRDGEENISGLKFMATHWVFSSLSFKNV KHKLSTDFHKETLLIQIVDELSKVPDEVYKTFDKQTQEEFIEDINEYMKV GNKDLSLEESTVIHPVIRKRYDNKFNYFALRFLDEFAGFPTLRFQVHIGN YIHDRRIKNIDGTAFQTERSVKERIKVFGKLSQMSNLKAEYVSGLMDEP VDTGWEIFPNPSYNIIENNIPIYIEMGDHFNDEVLQSKMARKKQKPEE LKDRNSAKASKESMIQTLQNDKGLMDVITVSPTAQLSLNELPAILYELL VKKTPAKTIEKKLVGKLNQRLKEIKNYNPEKPLPASQISKRLRLNREEGSI NTKKIIALLQKELNYTQEKLDLLEKNRKEYGKKVDGKILRKYVFGLKEIG NLATDMAMDIKRFMPANVRKEWKGYQHSQLQQSLAFYDKRPEEAF NILQEVWDINREKSLWDTWILNAFQTSGNFERFFELYHEGRKKYIQQ QLENIDRYTDNKKFLQKFINQQFPTNFLEKRLYTLESLEIEKLKILSKPFIL PRGTFDEKPTFIMGEKVTENPELFADWYTYGYQQHEFQKFYSWPRDY KDLLQNEQKRDPDFAENKKGLSDLKQLELLQLKQDIIIKKIKTQDLYLKLI MDALFIEVFGQEADISLNDLYLTQEERLEKEKLALKQHQRVEGDDSPN VIKDNFIWSKTMPYKHDKIYEPQVRLKDFGKFKHFLLDDKVAKILSYDL QETWNKNELEIQINTGQDSYEVIRREELLKEIQLLEKQILETFSHTLDEH PKEFEDEKGNPNFKMYMANGVIRKGSSTTAKDEADWLEHEKDFDNL SLEIFNSKSEITQLTFLIVLIRNKFGHNQLPIKQFYEIIQNEYSITGETISRLY LNFIIYAKARLKDLM |
| 26 | Cas13 | MEEKLGKGVEYNPFKKEDKYYFGGYFNLAENNINEVFKEVKKRLGETN SSSNIELLNNVFRKEMSLVDYEKWVNAFADYFPIVNYLDRETIKKGEKV VEVPREKRIECFRDMFKGLINTISQLRHYYTHYHHEPIEIDDKILSFLDEV LFNTIITTKNKYLKTDKTKELIKDSLQEELDILCKLKVKYLESKRKRFDRKD KGAIENAVYNDVFRRFIYKDEKGNESLKDIIRTKQIKVHQNSSYLELPISS SGIIFLLSLFLNKKEVESLKSNIRGYKGKSKSEETTPEKNGLLFMTTHRIYS VLAYKGLKKRIKTSVKGDKETLLMQMIDEVSKVPHCIYQNLDQTLQAT FIEDWNEYFKDNEENEENLENSRVLHPVIRKRYEDKFNYFAIRFLDEYA EFPSLRFQVNLGNYVHHKATKKFGNSEVTTERVIKDKITVFGRLSEVNK AKADFFKNETELDPAWELFPNPSYEFPKEKGNNDKDAGKIGIQVKLLN KDIEAVLNESKNTLNNKTRKSDKISKKEIINKIVQINDDTKYNNKNIIYQ GNAIAYLSLNDIHSLLYELLVIGTKGDKLERKVVEKIQQQVTEIRNKDTS AKILSKYKDSEESNTIDKKKLVIDLKYEYDKLQDLLKEHKNREEDYIQTKK KKKDSPKRKYILYHNEKGQVAVWLSNDIKRFMPQNFKEKWKGYQHS EFQKSLAYYETNKEMLKIILQDLDLEQFPFDIKSCFYKNTLEDFYNRYLSL RISYLENVIDRVECFSNEPKAFKSVLKECFVFLKKQNYTNHSLDEQVKKI LANPIFIERGFLDTKPTMIQGVKFSENKGCFADWFVHYKEYEHYQKFY DTNLYPVESIEDKERQKLEATIKKQQKNDVFTLLMIKKIFNDLFNQDFE ANLYEMYQSKEEREKNQLVAKETQNRNLNFIWNKPIAIDLFDGKVKID EVKLKDVGSFRKYENDKRVQTFITYIPEIQWIPYLPNTWEGINLPVNVIE RQIDRYEKVRSEELLKEVQAIEKYIYEQVNDKTELLQNGNQNFKNYLVN GLLKQIQGIDVSNFKFINQQKFETINVKDLDNEASALEQKVYVLINIRN QFSHNQFPKSAFYQFCQKILSIEEDELFADYYLRLFKLLRNELLD |
| 27 | Cas13 | MNTRVTGMGVSYDHTKKEDKHFFGGFLNLAQDNITAVIKAFCIKFDK NPMSSVQFAESCFTDKDSDTDFQNKVRYVRTHLPVIGYLNYGGDRNT FRQKLSTLLKAVDSLRNFYTHYYHSPLALSTELFELLDTVFASVAVEVKQ HKMKDDKTRQLLSKSLAEELDIRYKQQLERLKELKEQGKNIDLRDEAGI RNGVLNAAFNHLIYKEGEIAKPTLSYSSFYYGADSAENGITISQSGLLFLL SMFLGKKEIEDLKSRIRGFKAKIVRDGEENISGLKFMATHWIFSYLSFKG MKQRLSTDFHEETLLIQIIDELSKVPDEVYHDFDTATREKFVEDINEYIR EGNEDFSLGDSTIIHPVIRKRYENKFNYFAVRFLDEFIKFPSLRFQVHLG NFVHDRRIKDIHGTGFQTERVVKDRIKVFGKLSEISSLKTEYIEKELDLDS DTGWEIFPNPSYVFIDNNIPIYISTNKTFKNGSSEFIKLRRKEKPEEMKM RGEDKKEKRDIASMIGNAGSLNSKTPLAMLSLNEMPALLYEILVKKTTP EEIELIIKEKLDSHFENIKNYDPEKPLPASQISKRLRNNTTDKGKKVINPE KLIHLINKEIDATEAKFALLAKNRKELKEKFRGKPLRQTIFSNMELGREA TWLADDIKRFMPDILRKNWKGYQHNQLQQSLAFFNSRPKEAFTILQD GWDFADGSSFWNGWIINSFVKNRSFEYFYEAYFEGRKEYFSSLAENIK QHTSNHRNLRRFIDQQMPKGLFENRHYLLENLETEKNKILSKPLVFPR GLFDTKPTFIKGIKVDEQPELFAEWYQYGYSTEHVFQNFYGWERDYN DLLESELEKDNDFSKNSIHYSRTSQLELIKLKQDLKIKKIKIQDLFLKLIAG HIFENIFKYPASFSLDELYLTQEERLNKEQEALIQSQRKEGDHSDNIIKD NFIGSKTVTYESKQISEPNVKLKDIGKFNRFLLDDKVKTLLSYNEDKVW NKNDLDLELSIGENSYEVIRREKLFKKIQNFELQTLTDWPWNGTDHPE EFGTTDNKGVNHPNFKMYVVNGILRKHTDWFKEGEDNWLENLNET HFKNLSFQELETKSKSIQTAFLIIMIRNQFAHNQLPAVQFFEFIQKKYPEI QGSTTSELYLNFINLAVVELLELLEK |
| 28 | Cas13 | METQILGNGISYDHTKTEDKHFFGGFLNTAQNNIDLLIKAYISKFESSPR KLNSVQFPDVCFKKNDSDADFQHKLQFIRKHLPVIQYLKYGGNREVLK EKFRLLLQAVDSLRNFYTHFYHKPIQLPNELLTLLDTIFGEIGNEVRQNK MKDDKTRHLLKKNLSEELDFRYQEQLERLRKLKSEGKKVDLRDTEAIRN GVLNAAFNHLIFKDAEDFKPTVSYSSYYYDSDTAENGISISQSGLLFLLS MFLGRREMEDLKSRVRGFKARIIKHEEQHVSGLKFMATHWVFSEFCF KGIKTRLNADYHEETLLIQLIDELSKVPDELYRSFDVATRERFIEDINEYIR DGKEDKSLIESKIVHPVIRKRYESKFNYFAIRFLDEFVNFPTLRFQVHAG NYVHDRRIKSIEGTGFKTERLVKDRIKVFGKLSTISSLKAEYLAKAVNITD DTGWELLPHPSYVFIDNNIPIHLTVDPSFKNGVKEYQEKRKLQKPEEM KNRQGGDKMHKPAISSKIGKSKDINPESPVALLSMNEIPALLYEILVKK ASPEEVEAKIRQKLTAVFERIRDYDPKVPLPASQVSKRLRNNTDTLSYN KEKLVELANKEVEQTERKLALITKNRRECREKVKGKFKRQKVFKNAELG TEATWLANDIKRFMPEEQKKNWKGYQHSQLQQSLAFFESRPGEARS LLQAGWDFSDGSSFWNGWVMNSFARDNTFDGFYESYLNGRMKYFL RLADNIAQQSSTNKLISNFIKQQMPKGLFDRRLYMLEDLATEKNKILSK PLIFPRGIFDDKPTFKKGVQVSEEPEAFADWYSYGYDVKHKFQEFYAW DRDYEELLREELEKDTAFTKNSIHYSRESQIELLAKKQDLKVKKVRIQDL YLKLMAEFLFENVFGHELALPLDQFYLTQEERLKQEQEAIVQSQRPKG DDSPNIVKENFIWSKTIPFKSGRVFEPNVKLKDIGKFRNLLTDEKVDILL SYNNTEIGKQVIENELIIGAGSYEFIRREQLFKEIQQMKRLSLRSVRGMG VPIRLNLK |
| 29 | Cas13 | MENQTQKGKGIYYYYTKNEDKHYFGSFLNLANNNIEQIIEEFRIRLSLK DEKNIKEIINNYFTDKKSYTDWERGINILKEYLPVIDYLDLAITDKEFEKID LKQKETAKRKYFRTNFSLLIDTIIDLRNFYTHYFHKPISINPDVAKFLDKN LLNVCLDIKKQKMKTDKTKQALKDGLDKELKKLIELKKAELKEKKIKTW NITENVEGAVYNDAFNHMVYKNNAGVTILKDYHKSILPDDKIDSELKL NFSISGLVFLLSMFLSKKEIEQFKSNLEGFKGKVIGENGEYEISKFNNSLK YMATHWIFSYLTFKGLKQRVKNTFDKETLLMQMIDELNKVPHEVYQT LSKEQQNEFLEDINEYVQDNEENKKSMENSIVVHPVIRKRYDDKFNYF AIRFLDEFANFPTLKFFVTAGNFVHDKREKQIQGSMLTSDRMIKEKINV FGKLTEIAKYKSDYFSNENTLETSEWELFPNPSYLLIQNNIPVHIDLIHNT EEAKQCQIAIDRIKCTTNPAKKRNTRKSKEEIIKIIYQKNKNIKYGDPTAL LSSNELPALIYELLVNKKSGKELENIIVEKIVNQYKTIAGFEKGQNLSNSLI TKKLKKSEPNEDKINAEKIILAINRELEITENKLNIIKNNRAEFRTGAKRK HIFYSKELGQEATWIAYDLKRFMPEASRKEWKGFHHSELQKFLAFYDR NKNDAKALLNMFWNFDNDQLIGNDLNSAFREFHFDKFYEKYLIKRDEI LEGFKSFISNFKDEPKLLKKGIKDIYRVFDKRYYIIKSTNAQKEQLLSKPIC LPRGIFDNKPTYIEGVKVESNSALFADWYQYTYSDKHEFQSFYDMPRD YKEQFEKFELNNIKSIQNKKNLNKSDKFIYFRYKQDLKIKQIKSQDLFIKL MVDELFNVVFKNNIELNLKKLYQTSDERFKNQLIADVQKNREKGDTSD NKMNENFIWNMTIPLSLCNGQIEEPKVKLKDIGKFRKLETDDKVIQLLE YDKSKVWKKLEIEDELENMPNSYERIRREKLLKGIQEFEHFLLEKEKFDG INHPKHFEQDLNPNFKTYVINGVLRKNSKLNYTEIDKLLDLEHISIKDIET SAKEIHLAYFLIHVRNKFGHNQLPKLEAFELMKKYYKKNNEETYAEYFH KVSSQIVNEFKNSLEKHS |
| 30 | Cas13 | MEKTQTGLGIYYDHTKLQDKYFFGGFFNLAQNNIDNVIKTFILKFFPER KDKDVNAAQFLDICFKDNDADSDFLKKTKFLRMHFPVIGFLASNNDK AGFKRKFSLLLKAISELRNFYTHYYHQPIEFPSELFELLDDIFVETTSEIKKL KKKDDKTQQLLNKNLSEEYDIRYQQQIERLKELNAQGKKIPLNDETAIR NGVFNAAFNHLIYKDGGDLKPSRVYQSSYSEPDPAENGTSLSQSSILFL LSMFLERKETEDLKSRVKGFKAKFIKNGEEKISNLKLTATHWVFSYLCFK GIKQKLSTEFHEETLLIQIIDELSKVPDEVYSAFGAKTKQKFVEDINEYMK EGNADLSLEDSKVIHPVIRKRYENKFNYFAIRFLDEYLSSTSLKFQVHVG NYVHDRRIKNINGTDFQTERVVKDSIKVFGRLSKISNLKADYIKEQLSLP NDSNGWEIFPNPSYVFIDNNVPIHIQTDEATKNGIKLFKDTRRKEQPEE LQKRKGKLSKHNIVEIIFKETKGKDKPRVDEPLALLSLNEIPALLYQILEKG ATPEDIELIIKNKLAERFEKIKNYDPETPAPASQISKRLRNNTTAKGQETL NAEKLSILIEREIEDTETKLDAIEEKRRKAKKEYRRNSPQKSIFSNSELGRI AAWLADDIKRFMPAELRKNWKGYQHSQLQQSLAYFEKRPQEAFLLLK EGWDTSDGSSYWNIWVINSFSETEDFEKFYENYLRKRAKYFSELAGNI KQHTHNAKFLRKFIKQQMPADLFPKRHYILKDLETEKNKVLSKPLVFSR GLFDSNPTFIKGVKVTENPELFAEXXNGIATGTKRNIPSSISMAGKETI MSF |
| 31 | Cas13 | MEQNKLGKGIDYNPFKTVDKHYFGGFFNLADNNIQEVFDEINIRYKN GNLKPKVAIERYTTENTSLVEYEKFVAILTEYFPIVKEIDQKNKKDSNDK VIEKTRIERITDFRDAFILFIETIEKLRSYYTHYQHDDITIDNQLFIHLDKILL NTVLETKKKYLKTDKTKELLKNSLQAELKELYHLKINQLEQKKNEVDALI KEQKSKGKKTDKPFKYSKDRDQIINSIYNDAIRPFLYENANKVELSDKKK TAFNEKDASASERDFNLPISSSGIIFLLSCFLNRKEIEDLKANIKGYKGKVI KGETFDLEKNSIRFMATHRIYSVMCYKGLKNKIRTSESATKETLLMQMI DELSKIPDIVYKNISTDLQNTFTEDWNEYYKDNIENNENLENSKVIHPVI RKRYEDKFNYFAIRFLDEFVDFPSLRFQVHLGNYIKHSMPKNIGSVTTT REIKNKIFVFGKLNEINQSKNDFFNKNKEEEQETNWEIFPNPNYHFPM ENSDELKNANKIGIYIDLKDKRKKDTLNEAIKKREKETSIYKKDLVHQIID KNLDMHIGQPVAYLSMNDIHAIIFSILSQNVFTKDNKLNGGDIEKKIKD QINNQITEITEKDASIKILKNHSDNNSNYPNTHKLYDDISNEIEXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXXXNEIEVLDKLMQKHEKRVKEY INTQEDKKYKPARKHILYNSEKGEIATWLANDIKRFFPKEFKENWKGH YHSEFQRNLAYYETNKKEVKTILNDLDYRKEIPFIDFSKNTLADFYFEYLK KRKIYHKNLWVEVNKLIKGENINKEKLFDNCFRIYKRKNYVSNVIDEKV NTILSNPIFIERGFIDEKPTIIPKMPLEGNEEHFAAWFVAFKSFKNNEFQ NFYDTNKYPLETKDKTNSELKKIQTKTYNQKKNDWATWLIVQYIFKDIF STDLQNVKLSELFQTREQRIQNQVKALDGERNQNFIWNRTIDLQLNE KIKIPNVKLKDIGNFRKYVNDSRVEAFLRYNDITQWMAYLPSNWQKE DESKPKPVNVIQLQLDDYEKIRREELLKEVQKLEKTIYNNTNVKTVLLQ DGNPNFKNYVLNGLLEEIKGINISAFTVLHEKTNFDKIDFNVLENCSEIE QSATLIILIRNKFAHNQLPSSDCYQFCSKILTRDTEQTYANYYLKLFMILK DKL |
| 32 | Cas13 | MEETTTMGKGVAYDHTLFKDKHYFAGYLNLAVNNIENVFKTVYKNRF DIKQHNLYKILDSLDGQISEPDYIERVSFLKQYFPVLHYLDLHPDNKRFT KEEDKVKARRRYLINNLRLLIETLSKLRDFYTHYYHKPLSIEQNTFSLIDNI FLNVVIDVKRQKKKNDHTRQLLKDSLKEEMDILYQKTKASLKEKQKEN TRIKLDSETINNTIFNNSFSHLIYRRKKADNDILSASCKSEYKGEPTENGI NVSVDGLLFFLGIFLSRKESNDLRGRIKGFKGTVIKDLPDFPNEKNNSLK FMATHWVFTYLNIKPIKQKLNTNFSRETLLLQIVDELTKIPNEIYRNLCF KKQQEFVEDINEYIKEGDDIDTLNSSTVIHPVIRKRYENKFNYFVLRYLD EFVSFNSLRFQIYLGNYVHHIQRKKLSGTEYETERVIKEKINVFGKLSEVS NIKGDYFIQNNPDNEALGWEIYPNPSYNFTGNNIPIYFDINDQDKEKIN EYKSIRNFSEKRILRKKNKKNKQEIFDLINNTLTTRVFTAEPTAILSLNELP ALLYTILCENKTASEIENLLRRTYLKRLNTIKNYQPGTLPQSKITKNLNKS TNQESLDVSKLIKAMKHEISISNEKLTLIKKNQNEVKDTSHRRKYVFNSK ELGIEATWLANDLKRFMPKKVRENWKGYMHSQLQNSIAYYSQKPKE ALSILSSVWNFNDDNYIWNEGIKKAFNEKEFEKFYCKYLASRNKTLEKL KENLDNLEYKTDKRKLDKFIKQQNLDCLFHIRTYTIDSTQEQINKLLAKP LVFPRGIFDSKPTFVKNESVTEKPELFADWYTYTYKEHPLQEFYSFTKDY ECNFKKEKLTVKEFVKNQEQLNPEEQLNLFKLKEDLSIKCIKNQDLFLKL VVDNIYNKIFEYNIDISLKNLYISRKERIAIGLKAKELNQINDSYIWGKTIL YQDKQIRETKVQLKDINKIKRFLEEDKVKQILSYDINKQWEIEELKYELYI KPNSYEVIRREKLFKAIQEFESYILTINNFDGSNHPSILEYNSNPRFKHYV VNGLLLKKGLATNEEIEWLLAKGQKEFNTFDKSIVEKPEIIQKAFLLVLIR NKFAHSQLPIKEYYEMIRSYTKNIENLNTTEIIFQFTTNTINELKR |
| 33 | cas12b | MATRSFILKIEPNEEVKKGLWKTHEVLNHGIAYYMNILKLIRQEAIYEH HEQDPKNPKKVSKAEIQAELWDFVLKMQKCNSFTHEVDKDVVFNILR ELYEELVPSSVEKKGEANQLSNKFLYPLVDPNSQSGKGTASSGRKPRW YNLKIAGDPSWEEEKKKWEEDKKKDPLAKILGKLAEYGLIPLFIPFTDSN EPIVKEIKWMEKSRNQSVRRLDKDMFIQALERFLSWESWNLKVKEEY EKVEKEHKTLEERIKEDIQAFKSLEQYEKERQEQLLRDTLNTNEYRLSKR GLRGWREIIQKWLKMDENEPSEKYLEVFKDYQRKHPREAGDYSVYEF LSKKENHFIWRNHPEYPYLYATFCEIDKKKKDAKQQATFTLADPINHPL WVRFEERSGSNLNKYRILTEQLHTEKLKKKLTVQLDRLIYPTESGGWEE KGKVDIVLLPSRQFYNQIFLDIEEKGKHAFTYKDESIKFPLKGTLGGARV QFDRDHLRRYPHKVESGNVGRIYFNMTVNIEPTESPVSKSLKIHRDDF PKFVNFKPKELTEWIKDSKGKKLKSGIESLEIGLRVMSIDLGQRQAAAA SIFEVVDQKPDIEGKLFFPIKGTELYAVHRASFNIKLPGETLVKSREVLRK AREDNLKLMNQKLNFLRNVLHFQQFEDITEREKRVTKWISRQENSDV PLVYQDELIQIRELMYKPYKDWVAFLKQLHKRLEVEIGKEVKHWRKSL SDGRKGLYGISLKNIDEIDRTRKFLLRWSLRPTEPGEVRRLEPGQRFAID QLNHLNALKEDRLKKMANTIIMHALGYCYDVRKKKWQAKNPACQIIL FEDLSNYNPYEERSRFENSKLMKWSRREIPRQVALQGEIYGLQVGEVG AQFSSRFHAKTGSPGIRCSVVTKEKLQDNRFFKNLQREGRLTLDKIAVL KEGDLYPDKGGEKFISLSKDRKLVTTHADINAAQNLQKRFWTRTHGFY KVYCKAYQVDGQTVYIPESKDQKQKIIEEFGEGYFILKDGVYEWGNAG KLKIKKGSSKQSSSELVDSDILKDSFDLASELKGEKLMLYRDPSGNVFPS DKWMAAGVFFGKLERILISKLTNQYSISTIEDDSSKQSM |
| 34 | cas12b | MATAVDTSTTRAYTLRLSGGNNWRELLWQTHVAVNRGAWVWGD WLLTLRGGLPASLADGDAERRVVLALSWLSVESPASLAPQAHIVAYGS DARDERNRKVTERFRDILRRMGIKQQQEQEWLDACLPALMASIREDA VWVDRSACFAEAQQCYRGLSSEWARKTLFDFLGGEDDYFKPSAKEG ASSKAKDFVQKAGRWLSRHWGAGKKSDPRDISTRLGKLAGVDPKAID GHTGRAALEDLLRTLGSRPAQNADAEKLYRQLKRAVGWKGRPSKGA VALKKIRDAERVPNDLWKEIASTLREEAAVQSSQTSDHAAVPDWRSH WPAEITGLPMPYRVDRDYIWEHGVMLDHALRRVSSAHTWIKRAEAE RRRFQQDAAKMGSIPEEARNWLDAFRERRSSSSGATGDYLIRERAIN GWDKVVQAWETLGPNSTRDQRIAAARDVQANLDEDEKFGDIQLFA GFGDEHVDDPERCLADDRATCVWRNSSGRADGRILKDYVAATVAEH NQRRFKVPAYRHPDPLRHPVFVDYGKSRWSINYSALTAAQQRRKTTQ KLAQAKTDNTRAKLQQQLASTADLRSVTLGVWDGNRIVKISQRWRS KRFWRDLDLDHFGSHPSAAVSRADRLGRVAARQDPGAAVYVAKVFE QQDWNGRLQVPRRELNRLADVVYGKGADPDFGKLERLDPRARRLW ERLSWFLTTSATVQPQGPWLDYVAAGLPSGIQYTKSRAGYYLNYDAN HGRKGRARLCLARLPGLRVLSLDLGHRYAAACAVWQTLTIEQMTNEC RQAAHPAPSNDDLFIHLRHPTHKPQKSGRKKGRPVTKTTIYRRIGPDK LPDGTDHPAPWARLERQFLIKLQGEDRPARYASQKEIDEVNQFRNFV GLEPIVDRPRVDDLHSDAVRVARLGLRRLADAARIAFAMTAAKKPISG GHEVELTTAQRIEFLQDALLLWQSLAASRRYRDDWAEKLWQSWVVE KLGGPQPAEIADDLPRSQRAASLKTARQSLRKVAEKLSDGQSPSAAEL HRLWAERWQQRQTEWRRHLRWLRRLILPRRKDHQQEDRPLQRVG GLSVKRIQTIRQLYQVLKAFRMRPEPSDLRKNIPAPGDRSLASFGRRIL NHLERLREQRIKQLASRVVEAALGAGRISKPPGRDRRRPQQPVDRPC HAVVIENLQHYKPEDSRLRRENRQLMDWQARNLRKYIVEGCELHGLL FVEVSPAYTSRQDSRTGAPGLRCEDVSRTALQEAARRMHASHSRPSN SSPGGSQTQFEREVCRWINEFKRVEGSSSSLSARQAVLKAFLHHQASI PTSLSTILLPRRGGELFVSADPDSPLACGLQADLNAAANIGLKALTDPD WMGAWWFVLVDRASGQPVEEQVQGCPIWLSCGPLSNSNPATIDPS DSPTAARRSNGTGAKGRARANEYWWSSLSATTLPDHKAWQPTQDY WRDIEQRVVKRLLRLLDGSEWSED |
| 35 | cas12b | MNRIYQGRVSKIEIKDSEGNFRNVPVGSPDTCPLWRHHRIFQDAVNY YLVALGALAGTGSENAFVGLGSKDRVIHDLYSRLFDSWERFPRDMHG ASSLRDSLRRTLPGLSERASLQDAFDAILSGNEANARERVLSLLSLIQDL GGDIQKGSKRYFPFFCEPATKATFPRARVGLLKVEGKDFVPRLLWSSD LEIAPDQVVEQLKFEYFANPNESVQPIEGNEARVRLIEALDNPQLGIEL PIEILSDLRKRVHLIETDIRIPRYFFGGAGAELRKFRLDLFLIAAYVTPDPSI LRALRNSFKEPSASKSSKKKDETEEVENLLRSLGDDPLILARGERGFVFP SFTSLPTWVGANAQKPIWRDFDIAAFAEALKSLNQFTAKTEEREEKLK KAEETLHYMLGISDAIPRSSDSETEEQAPSRPGKDPRWPLVAQLEKEL GENLSEGTWQLSRSAMRGLRDIIGLWRKHPGASVVTLQKDVKTYQA DEKHKREIGSVQLFLLLCEERYHALWQTETDDERGDESEENDDPARILS DAIEVHQIRREVERFREPIRLTPAEPVFSRRLFMFSDLTDKLAKVKFGET TEENSEVKSQFVEAAIALKEGENLKEARVRITFSAPRLHRDELLGGAESR WLQPITAALGFSNPAPSVKFDSAVALMPDHMDDGRIRHLLNFPVNF DSAWLHQSIGKADLWKSQFNGTKDKNLHLHWAGTARDTTRKNTW WENRTIIENGFTVLSNDLGQRSAGAWALLKVTCSRPDTKHPVRSIGH DGTREWFATVLATGIHRLPGEDQRILKNGKWATEQSGKKGRNATFSE YEAACVLAKNLGCESVENWLGMSGEKSYPALNDQLVKIANRRITRLGT YHRWSCFSPEKFEDPARRANVIGGQLAELSAYQDENVTVSADILKSGD FEGFRHRAGAAFEALRTELEVHLVNLANLTAPLRQKVWSWQKRPDSS GYGDLLMVDLDDCHPKIRGQRGLSMARLEQLEGLRRLFLRYNRSLDR SPGIPAKFGREDVGRTSGEPCQALLVKIDRMKEQRVNQTAHLILAQAL GVRLCPHRIEENERKSRDLHGEYEKIPGREPVDFIVIEDLSRYLSSQGRA PSENSRLMKWAHRAVRDKLKMLAEEPFGIPVVETVPAYSSRFHALNG QAGSRLHELHELEAYQQQSLINLAAKTDFQNRDRSKAAGELFEQFQA LAKLNERRRAEGKKVPRTLYYPKSGGPLFLASRDGDTIHADVNAAINL GLRAIAAPACIDIHRRLRATKEKEVYRPRVGNAREKSAFSKDDIIQPSGA PSKKFASSSSPNFFYEPEDLKQANGEPLFDRAMFGEYSLVSGVSLWSM VNNAIYIRCVELNRTRLHGKDPDDQIPM |
| 36 | cas12b | MSITRSIKVKLIVPRDASLEARQLREGLWATHLFVNDGCHYYERLLLEF RQRDVCVGKDDAGKDVIVPAAEWADRLRARLGRNGMVPSHIEAALP IFRELYENMVPSALKAKSGTGQAGRSWHSKLVSPTSRGGEASAARIDV LRPLLPVSGDDPAFEPAARALIEEAGDELLTSTGRCPAWVTAYRKGPE GSAWVEKLRIQLREAVEAGDFDPPSDPQILAAGAVPAAPPLGAGIDAL RPLLPLLGGDPAFEPAARALVEDIGDELFTSTGRPPTWVTAHPTWVRA HRKDAECLEAADDFKWVERLRQRLRDDAKAGKFEQPLHERLGALGAL PVAKPIGAGRVVSRADLTVFERGAMELAIEHLIGWESAGHRARAQYV ERKKRHDDLLQWIEAEAPDALLAVRAYEAARTIHLATLGELGAAPQYT LRLREIRPWRKLREWLLQNPDATIDERRRRLATMQTNDPRGYGGEAL AWLAAPERRALVEHPAGDVVTRIAVLNIRKSILDRSRLFPTCTLADPVE HPRFAKFGKPGDKNSAGYALAVDGVRREAIIKILVPRQDGLLVPTDLR VPFAPSGQMRDLRASGLDISYERQDGRGRQAAKLQGGNLMFDRTHF ARCGAPGPEALGSVWIKVALDLSSPAASLAMKTATPVRTYLSTAVRGR PESTKYEKAAPPEGFRVLSVHMGLRTAATASMLRFGAPEEGGHEVPV SGLAGETLVAFHERTVTMKLPGEDPDTRTEANRGVAKRELRGLGRGI GCLKAIRRASASATPEDRAEALVIIETHVGQGDRHGWAPAEAVGRLD PHGDPDDWKTACAALYAAVEADLGVAISSWRKAARAGGATGMLGG KSLWAVDHLERSFRFLRSWDLRARPHDGDPRRPRPGYASKLLHHIDG VKDDRVKTTADRIVQAACGRAWIGGPTVKRGTQDVRLPGRWEQRG PRADLILLPDLTHFRFRSDRPRAENSRLMRWAHRQLAIYVRMQAEVE GILVADTGAAFTTRFDAWTGAPGVRCEPVTADHLRGIAKREDYWLAR LLREGALKHLRIDPASLRVDDLVPMDHGKILVALDGVDLPGLRILDTDV NASQGLGRRYIEGHGLAYRLPGARVPRGEGEREAAVVHIKGKRLASA MGGTVVVLRASEGPGDITWTAEVYDRPQGARKALGLSLAAFNSIATA AVDDEGPAPENDDEALEEEAEEALGIATGERIVFFRDPSGAVAGGGW LEASAFWGIANRMVTDRLRELGRLG |
| 37 | cas12b | MSLNRIYQGRVAAVETGTALAKGNVEWMPAAGGDEVLWQHHELF QAAINYYLVALLALADKNNPVLGPLISQMDNPQSPYHVWGSFRRQG RQRTGLSQAVAPYITPGNNAPTLDEVFRSILAGNPTDRATLDAALMQL LKACDGAGAIQQEGRSYWPKFCDPDSTANFAGDPAMLRREQHRLLL PQVLHDPAITHDSPALGSFDTYSIATPDTRTPQLTGPKARARLEQAITL WRVRLPESAADFDRLASSLKKIPDDDSRLNLQGYVGSSAKGEVQARLF ALLLFRHLERSSFTLGLLRSATPPPKNAETPPPAGVPLPAASAADPVRIA RGKRSFVFRAFTSLPCWHGGDNIHPTWKSFDIAAFKYALTVINQIEEKT KERQKECAELETDFDYMHGRLAKIPVKYTTGEAEPPPILANDLRIPLLRE LLQNIKVDTALTDGEAVSYGLQRRTIRGFRELRRIWRGHAPAGTVFSSE LKEKLAGELRQFQTDNSTTIGSVQLFNELIQNPKYWPIWQAPDVETAR QWADAGFADDPLAALVQEAELQEDIDALKAPVKLTPADPEYSRRQYD FNAVSKFGAGSRSANRHEPGQTERGHNTFTTEIAARNAADGNRWRA THVRIHYSAPRLLRDGLRRPDTDGNEALEAVPWLQPMMEALAPLPTL PQDLTGMPVFLMPDVTLSGERRILLNLPVTLEPAALVEQLGNAGRWQ NQFFGSREDPFALRWPADGAVKTAKGKTHIPWHQDRDHFTVLGVDL GTRDAGALALLNVTAQKPAKPVHRIIGEADGRTWYASLADARMIRLP GEDARLFVRGKLVQEPYGERGRNASLLEWEDARNIILRLGQNPDELLG ADPRRHSYPEINDKLLVALRRAQARLARLQNRSWRLRDLAESDKALDE IHAERAGEKPSPLPPLARDDAIKSTDEALLSQRDIIRRSFVQIANLILPLR GRRWEWRPHVEVPDCHILAQSDPGTDDTKRLVAGQRGISHERIEQIE ELRRRCQSLNRALRHKPGERPVLGRPAKGEEIADPCPALLEKINRLRDQ RVDQTAHAILAAALGVRLRAPSKDRAERRHRDIHGEYERFRAPADFVV IENLSRYLSSQDRARSENTRLMQWCHRQIVQKLRQLCETYGIPVLAVP AAYSSRFSSRDGSAGFRAVHLTPDHRHRMPWSRILARLKAHEEDGKR LEKTVLDEARAVRGLFDRLDRFNAGHVPGKPWRTLLAPLPGGPVFVP LGDATPMQADLNAAINIALRGIAAPDRHDIHHRLRAENKKRILSLRLGT QREKARWPGGAPAVTLSTPNNGASPEDSDALPERVSNLFVDIAGVAN FERVTIEGVSQKFATGRGLWASVKQRAWNRVARLNETVTDNNRNEE EDDIPM |
| 38 | cas12b | MPTRTINLKLQISPKTDEGRKIRSALWTTHSEINKAVAEIEKLLLLCRGE KYYTTNSKDEEVEVPEPQVKTDALEMARAVQAKNGKAGTGSDEEVLS ALRMLYEATVPSSVLDDKGKPLSGDAQSIGGSYAGPICDPETCRIKDV DRLFESGPFAETASKKFTQLPAWFNEVTKKNFNKDEPEKFVKVGKDK DEKFYEIDLRQADAWYESPEVKDIVSKNKAFNKDKWWKNKRDGVDT WAAEFVKKQFDLRKDVRVSIREELWDRLGLLPLGSLYFKKPVGNKWN RMAFRLAIAHLLSWESWNHQTLAEYTKYTKYKDGLIELAGASRSLEVR FEPLRQYQKERHEELSRTSFVDDDRPFTIGARMIRAWGRVREAWRNK GDGIDERRQILADLQTELKGKFGDPHLFLWLAEAGRESLWRDEDVLTT FVEINIAQRDLERHRPYSLMTFADARLHPRWAMYEALGGTNLRNYEL TPEGKVKIPLLICEKDKLSEKTFTIPLAPSGQLKSLEIKSLPKKKVKISYASA HQFYAGIPGGSEILFDRLFMENRASSALANGSCGPAWLKLTVDVESKA PPEWLDKKGRVQTPPTVHHFKTGLANKSKHTDKLEPSLRVLSVDLGLR TFASCSVFELVDEKPAKGLFFETDHPHLWAKHERSFKLTLPGEEAGDD PKVAQARREAMDEVYSLRRDMYRLKDILRLKIISAPNERREKLESKIAE MREKQDARAVVTSNFFERLSEKCDLNPPMWEHSCNEIHRDAEKAFS ARIGEWRKRTRKRPGSWEEWRETRSYHGGKSYWMIEYLEAVRKLLIG WSTHGRDYGEINRQNKKRYGTVASKLLKHINKLKEDRTKAGTDLIIQA ARGYIPLPGKGWMEKYRPCRVILFEDLARYRFKVDRPRRENSQLMKW GHREIINEATLQGEIYGMVVETAGAGFSSRFHAKTGAPGVRCRYLKED DFENGAPKEFLVRQMKNLMKGDRLEPGLLVPWDGGELFATVDNGK PIVIHADINAAQNLQRRFWTRFADAYRVNAVEENDNWVVTDTGVRV LGALEMAVHGEADRKPRTGFTLHGTLQSGAELKAEGKKTDIKDVEED KDDSISSEIIELQDEKERKGRETFFRDPSGGILDPGKWYGSKRFWGRAK GAVTEALLDNQGANNALEEKPGNDELPF |
| 39 | cas12b | MATRSFILKIEPNEEVKKGLWKTHEVLNHGIAYYMNILKLIRQEAIYEH HEQDPKNPKKVSKAEIQAELWDFVLKMQKCNSFTHEVDKDEVFNILR ELYEELVPSSVEKKGEANQLSNKFLYPLVDPNSQSGKGTASSGRKPRW YNLKIAGDPSWEEEKKKWEEDKKKDPLAKILGKLAEYGLIPLFIPYTDSN EPIVKEIKWMEKSRNQSVRRLDKDMFIQALERFLSWESWNLKVKEEY EKVEKEYKTLEERIKEDIQALKALEQYEKERQEQLLRDTLNTNEYRLSKR GLRGWREIIQKWLKMDENEPSEKYLEVFKDYQRKHPREAGDYSVYEF LSKKENHFIWRNHPEYPYLYATFCEIDKKKKDAKQQATFTLADPINHPL WVRFEERSGSNLNKYRILTEQLHTEKLKKKLTVQLDRLIYPTESGGWEE KGKVDIVLLPSRQFYNQIFLDIEEKGKHAFTYKDESIKFPLKGTLGGARV QFDRDHLRRYPHKVESGNVGRIYFNMTVNIEPTESPVSKSLKIHRDDF PKVVNFKPKELTEWIKDSKGKKLKSGIESLEIGLRVMSIDLGQRQAAAA SIFEVVDQKPDIEGKLFFPIKGTELYAVHRASFNIKLPGETLVKSREVLRK AREDNLKLMNQKLNFLRNVLHFQQFEDITEREKRVTKWISRQENSDV PLVYQDELIQIRELMYKPYKDWVAFLKQLHKRLEVEIGKEVKHWRKSL SDGRKGLYGISLKNIDEIDRTRKFLLRWSLRPTEPGEVRRLEPGQRFAID QLNHLNALKEDRLKKMANTIIMHALGYCYDVRKKKWQAKNPACQIIL FEDLSNYNPYEERSRFENSKLMKWSRREIPRQVALQGEIYGLQVGEVG AQFSSRFHAKTGSPGIRCSVVTKEKLQDNRFFKNLQREGRLTLDKIAVL KEGDLYPDKGGEKFISLSKDRKCVTTHADINAAQNLQKRFWTRTHGFY KVYCKAYQVDGQTVYIPESKDQKQKIIEEFGEGYFILKDGVYEWVNAG KLKIKKGSSKQSSSELVDSDILKDSFDLASELKGEKLMLYRDPSGNVFPS DKWMAAGVFFGKLERILISKLTNQYSISTIEDDSSKQSM |
| 40 | cas12b | MYRGFCTVTATSGGWQSTTFLAGAQMADTTTRAYTLKLQGDRLAL WRNHVIFNNGVKAWGEWLLCLRGGLPASLADHRDSLDVSKGEISRTF KERTAAITPATIRQELKFKAATEKKVREEVASRRKKVTETAVAKELLAAR RSELRRILALSWLCPETPVQLVPQAAIVAAADDSDREQKVLDGFRQILK RKGVSDVAGWVQDCDATLRATIRSDAVWVDRTACFCSMPRAVRPS EVDAAKHLFRLFGSMSDYFATASASSGPAEPKDFANTCRDWVSSFW GGGEKSNKASILAALSAIAQIKPTRVVGKRGPAALAVIAGVLEQKPVDD SVEALARAIGWLSGRPSAARLAINAIAASPRVSQKLWDRLVLACEKDC GRQKSKLAFEGSASTIASALEPRLAGLTGMPYASTGRELIGEYATMLAF AMRRVSQIHTKAKQAEAERRSFAPEQARLALVPSAARKWLEDYVEAR TAASGAVDGYQLRKRALGGWADVVAAWSRCETSEDRIAAVRELQAD WEKAGDVQLFEALAADDAICVWQSANGKTAASILTDYVRAAVADQN ATRFKVPAYRHPDPLRSPTFVGFGNSQWSIAYSAQGEARERRKLLDRA SGSAKDAERAREGLAREAVLQNVSLDLWAGDKMVPTQFRWQSRRLL SDLALHSVPAMKGAKVTRATRFGRARIAAGPVLLDGIADDTPWNGRL QAPRRQLEDLARILDAKGLPFDDESKWPPKVRSRLKHLGWFLTHSAKL TPSGPWLDYVAGGLANGWKWAEGREGACLFREDNKDRKGRAKLILS RLPGLRLLSVDLGLRTSAAAAVWQVVSKRQLTAAKDGAKSVSDTDLF CLVRTGDRTQVYRRIGLSAWARLERQFLIRLDGEKAAARPATTNEWES LQSFRAWLGCGIERRPEKLPPVDSLQQSAERLCRLGLRRLSDLARVAYL LTAKERPIMGGRTAPLDEEGTVQAAQDALSILHALGSSEDFSDARLQG IWRTAIGDTPPLAARLTKKQRQELREALRPAAEKLRGKAALGKELADL WKERSAAWAKHLRWLRDWVIPRFDKRKNGERVRSARGVGGLSLDRI ATIRGVYQIMRAYASRAEPTNLRAGVERLEKAAAKKLRPEFGRRMLAK MERLRENRVKQIASRIVEAALGVGSEDRLHWERGRRRPTAAISDPRFA PCHAVVIENLENYRPDEKRTRRENRGLMSWAARAVGKYLAEGCQLH GLYLRQVSPAYTSRQDSRTGCPGLRCNDVRAQELLNPEGWIGRLVAR AAEAVKEGKATPRQRLLVTLAESARAGIAESAAVRIIAPGGQLFIAADP QSPASNGIHADMNAAANIGLVALLDPDWPAAWWRLPCKAATGYVD ESKVGGSEAVPLGRAILEVGAEAGKVYVNAWSDPQDSAVSRREWTD TKRYWRDVEERVVEILLASNRGGRRGKPGAVPF |
| 41 | cas12b | MATKSFEAKIVCKPDEKYTAEQKKQFLWFTHQVFNDGVRKVIPYVFK MKRGELGPEFQAIYYAITSSQDAIGKLEAVINPDWTSGKIGKSDPNKW KELLKYQELEKGFRQRLKEEGIKSTKKFRKELEDEKKKLAKEIGQKDIWA DAAAILRNKNLLLFNRDELLPNLPSEFRRKIYEMTIQLIHGHQELVANW EDEHAEWLIEKDKWEEEHPEYMNVRPIFEKFEKEQGKVKGSRIRWLA YLDFLSSKPELANWRGKAKETIPLTKEERAGFRKPGQHFAAFFNKNPEL QELDRLHKEYQEKFARTQSKRTPHPDGFKHRPTFTLPDAMRHPVWYS FKGATDPTKGSTYRNLDLENCTLDLKVLTAMEGEGRNPGGMIQYAFE PDERIKGFRYVGTTEKGKRAKGYIYYDPILEKERPAKIQGIKLVFRPPRP DGTAYLIFSCQIEDEKPKIKIWKDKEEESPGEITKRKKTEVYPPELITLAID FGQRHLGAITICKNNNGRPEPIRFIPAYPKRRKDRESKPVSAWLAKIPG LTFNAVGMHEKEISAGMSRRFQDPKSIRQAGEKEGRKSKGQHIPETET PWAHLREHIANMKEDHYKKAANLIIRTALQNGAQVILIENLRNYRPML ERTNLENRRRMQWAVRQTAKFLEDTARPLGLIVRQVSSAYTSRFCSSC GHPGARVSLPGQKNWEKFYAEKYGKERKMIAVAGGQFFCCPACKKII NADINASLNMHKVFYQNFIWPGKIDKKDTKNFIWQGKNYNWDQIA DDVQSFLDQKAGIKKEDDIPY |
| 42 | cas12b | MAYGSEAPDERNRKVTERFRIILSRMGINQQQEQEWLDACRPALTAS IREDAVWIDRSACFAEAQQHYPGLSSEWARETLFDFLGGENDYFALP DPEAAPSSEAKDFVQKAGGWLSRHWGAGKKSDSTAISTNLNRLAGV ESKAIVGRCGCDALAVLLTTLGGWPAKNADSGTLYHQLKQAVGWKG RPSRAAKALEKVRDAPEVTDALWRQTADTLRQEAVAQSSRAAGGSG VPAWMPAWREDMEARLGMPYRGARDYIWEHSVMLDHALRRVSSA HTWIKRAEAKRRRFQQDADKIGSIPAKAREWLDAFRERRFSASGALR GYLIRERAIDGWDRVVQAWASLGPNCTREQRIAAARDVQANLDEDE KFGDIQLFAGVGDEDEGDPQPCLADDDAICVWRDLNGRADSNILKDY VAATVAKHDQQRFKVPAYRHPDPLRHPVYVDYGNSRWSIEYSALKAA HQRRKTTEKLVQAKTDRARAKFQQKPADTPDLRGVTLGVWTGSSIEK VSLHWHGKRFWKDLDLDHFGRDPSATVSRADRLGRVAASQHPEAA VHVAKVFEQQDWNGRLQVPRHELQRLADLVYGKGGDPDFAKLGSL DERRTRRQWEHLSWFLTTSTTIQPRGPWLDYVAQGLPQGIQYKKGR NGYYLEYAANQGRKRRARLCLARLPGLRVLSLDLGDRYAAACAVWET LTREQITQECHQAGHPGPSQDDLFIHLRHRTGKPQKSGRNKGKPVTK TTIYRRIGPDLLPDGTPHPAPWARLQRQFLIRLQGEDRPARFASQHEID GSNRFREFLGLPPLADRPRVDDLHRDMVRLARLGLRRLADAARIAFA MTATKKPISGGREETLATEQRIEFLQDALVRWQALAASSRYRDDWAR QAWQEWIVEKLGGPQPAEIADELPRSQQATRVETARRSLREVAAKLS NPQSSSATELHGLWAARWQERQTKWRQYLRWLRRLILPRRKDYQQ ANRQVHRVGGLSVKRLQTIRQLYQVLKAFRMRPEPSDLRKNIPAPGD PSLASFGRRILHHRERLRQQRIKQLASRLVEAALGAGRISKRLGRDRRR PRQSVDAPCHAVVIENLERYKPEDSRLRRENRQLMNWQARNLRKYIV EGCELHGLLFVEVWPAYTSRQDTRTGAPGVRCEDVPRSVLEEATRRIR ALGSAPSGSSRGRSETRFEREVCRWIHEFNRVVGSSSGLSPRQSVLKAF LDHQAAIPTWRSTVRLPRRGGELFVSADANSPLANGLQADLNAAANI GLKALTDPDWMGAWWFVLVKRDSGQPVPQQVQGSPIWESCTRLSS PATVDSSDSPAGARRSKGRGARGRARATEYRWSPLSAMTMPDNKT WWPTRDYWPEIERQIADRLLREQIDPENRF |
| 43 | cas12c | MKKTSPLKRSALRTARRQIARGCLPIGNRDISTTRTRVLPLADSVADAV WNQARTAALTLRGFGSGSLFDLLLDLHASGLRLFSSNGEREGFLLKQK FDAGKFDRAAAKDVGEDMPKFTAANLRAALVAIPRGGGPDTDAKAL ATRLARAVGVKATKLDKPPKLLKDMAKELAMAFPTWKELSTANGEV GAVIDDVARMYGLRWPSLRRGWAFRLPEVTRELGSPTLAFDPDAPVI DETSATARFAAIVARYLPECGGLTDSAAAKGVQARITTTNANGLSWLF GVGLRGMRDLPVDTVADTLAIDVTRGRDALRALVNDIKALPRLGEFG DRVYVESRATLQGAVDSLIANYVGRLADLVASADALEHDQPRPPVLD DADWKPAIFDGMGFTPWEVEDMLDARPVEVARLRLALGVLAGTTPA VAGDFARALADVEAFGAWAARTEAVAALINARVKVLKAPESLRLRGV LGGGRWKAVVSIHPDEGEPAQVIPQLDTQLQALLDDGQRAFDVLVA DYTPTFAAALEHARSDMRASLADKGREAPSAESIDLLARRKLLDMVAR VTRRGSPSLGHAFLAACAVQGLTRPGTATERSLRGHILSGEQALFVHP YARARSIVRLEHAGLLRLDLDALLTAMERDAEQRADVREQIVLRFTRQ SLLLGGLPGRIRLAKVPWTQEAAAASGVRGAPWLKLHPDDAGTVARS EVIKAFTARFHLSANGLLYRLNRMRFLERYDIRCFIGDTLLFAPKAGAW TPPEQYRHGKYAHWLSHPDLPRTEGGAVDVVPAARWLTEASRRADE DGRASAVALLAQFPHEWVAACEFEGAPVYEGVFPCEGKIGGWMKRR GYRLAPPRHFAGELLAAFKDASVSPHGLTFEREMLREGTTVRELSRRV VAAYPIAVPTHPDAERPWSPLHLMGLDLGEAGLGVCLRHIGTGAETTL LLPVRKTRLLAHREEHYRRKVQPRQAFRKGYGDAMELAVKAAIGEVC GIIDNLIVRYRAVPVFESAVAQARGSNKMIQRVFAGVVQHYTFVANN GAAQTVRQSHWFGAGRWSYTYGADLLPAARQMTEKQLLKAKAEAV FRPAMGFPGVMASGYRTSLVCACCGEDVLDAVDAAAEGGQVALTT DAEGSGVLDLGGRSLRIKLEAPSPNPIVQKAARRKRRRTPWEALADRT WTLTHKTDRADLVATLRRGLRRPPASVQGHATSGWEFHCAACGHIA QADVNAATNLVRRYDDRVRKMEQARAHWDDPSVRAKLASELAERA AARS |
| 44 | cas12c | MLTTKFKLELPAGCPLREDAATFDECRKLYDVVEGCGNGTLTGFLFSVI LSGFRIFPDGKTAEIFANRSVYDEDEFRSALVEAVGAPLPRFTVKALIKR LQMEVRARGNKDNRFVAEVMMKEYRQTLCGKTLPKGVDESYVDRLF EEMARELTSRYRSWNELKGDLLGACKAVDAALRGFGDFPSLATMVTR AAARRLPKDSTIVFDPQSPCIDVQTIGVDAMPYAAVSTILSYPESVGEK RRDFVQNHLTTPSAAGLSWLFNRGLELFSEESVEELCRLFHVPEDQRT RIVQIQNAARATPRQSFFLKKGGAPLGYHDFRSAFAGRINSWTANYL NRLEELQGLLHDLTDELRLPDLVRNGEDFLATTDCRREEVEILCRSFSRE RDRAQTAVEHLIGADPLQVVSDVAAIEEYSRIVNRLCAIKEQIVNSLRQ AEDDKASRWTALWSEVKDEFQPWEKLIRLPKLNGMSGGVPPAQDEL ETILARYSDVGRGASEHFDAVMEWAAKTGAEGDVLKKFAETEQQRA DQRAPGKYDGRELALRLVLQRVARVVRDRSDACAENVRQWFLKENV FAERKDFNKFFFNRLGNLYVSPFSNRRHAGYKLSDGLVERSGAVWREL LALVKEMRGAYASFSEAGETFLRLESLLMGMRIGALTKNIPAEVAALRL DDETALESVSEGLKLQLQQAEVPPSVLAKAFNVYVSLLSGCLIALRRERF FLRTKFSFVGNTALVYVPKEKSWPMPSRYEASPSWTPIFENDVLVRLS TGEVEVAETFRRAVALWGRTTDPVLKKALRELFHQLPHDWCCQVSVR SSGDMTPAKRKEDDRDVLIVEKKGKYDSTIISKKIAATALVRLVGPSTH KERLNRLLLDVGEVACDMTLLADQEILQKVEDDRVHLSPGKLQFSLSV PISTPAEQCEDEVKSERKSTHFRRIVAIDQGERGFAFAVFRLEDAGKKG AQPIAQGFVNIPSIRRLIARVHSYRKGKQSVQKFSQRFDSTMFTLRENV AGDVCGAIAGLMSRYRAFPVLERQVSNLASGGKQLELVYKMVNARFL DDRIPMHSLERTSWWCGTSDWVIPDLWVEVPESYAVKAKKDEILEKD GKFYRTLRITPGAGVNAKWTSRICSQCGGNAMELIEKAREEKVKTVTL DANGEVTLFGRTLRLYKRPSEERSREARRRNERAPWTEPRADVRLSLD DFRRAVAENMRRQPKSLQSRDTSQSRYFCVFTDCRCHNKEQHADIN AAVNIGRRFLESLLRE |
| 45 | cas12c | MRRQHHGGQNARDWRRKVAAAALRQKESVFTYKFGLSVNDGDFDF DAAARTYDITEGIERGSLIGLVCAVHLSGFRLFSKVAETRQFLNRSRYPE NEFAQALAAHTEIENPSVTVQSIESVFVTPPRKQDGVARLWSADELAK RLFQTWNNRSPREGERNHPELLLAQGIARAVTKAFSGWKELADNAV HALTCADNYLATLGNRFPKLSDLPPLTAGSTQTGTLAFDPESPFLNMT GNEDIWLHQVVAVCAGRLKRYMPEIDPSSRKFASRLTDSIVSSQNNGL SWLFGNGLRFLRQSSIAQIAETLSVSQNEHRRVEQLKEFADAIPVNPFF ATDGYAEFRGSVGGKISSWVSNYWKRICELTVLHSQPPDITIPEGLLAS ENATLFSGQHTAAAGLVALSARLPSQVRDAGKALFVLSGDGVPRADD IATVEDVAGELAELTGQLAMLDNRIQQEIERAQDANDEGRVGSLASL RPNPTKELKEPPKLNRISGGTADAAGELARLETSLNDLIRARREHFYRLA EWTGNTASLDPLPALAERERKALTDRGMDPTLAEADEYALRRLLHRIA GMARRLSPNEAKRVRETMTPLFLKKREANLYFHNRAGALYRHPFSNS RHQPYSIDLNRARATDWLAWLEERAREMLGLLGSGAPANHEYLRDLL SIETFVFTTRLSGLPAQVPGYLAKPKSDLTNIPPLLAAQLDVDEVSRDVA LRAFNLFNSAINGLSFRAFRDSFIVRTKFLRLGHDELFYVPKARAWKPP ADYRSAKGKISKGLALPAVKRNEAGSILPRETTQGLSRAKFPEGSHALLS QAPHDWFVELDLRHDKMPQLAGLPVKMNADGLKGWRARRRPTFR LAGPPSFKTWLDRALTSTAVKLGDYTLILDQSFKQSLRVEDGEVRLSAE PAGIKAEIAVPVIDARPFPETEAEALFDNIIGIDLGERRIGYAVFSLPALLK SGNPTRVKPTVVGSVAIPAFRRLMAAVRRHRGSRQPNQKVSQTYSTA LQQFRENVVGDVCNRIDTLCERYRAFPVLESSVANFETGANQLKLIYG TVLRRYTFSNVDAHKSARSAYWYSANRWQHPYLFVREWNKAQRTFT GSAKPLAIYPGVTIHPAGTSQICHRCGRNALRALRNMPDRTIRVGKDG LIVLADSTIRLLERADYSDRELKTFKRRKQRPPLNMPVPEGARPRDQLE RVLRRNMRQQPQSEMSPDTTQARFTCVYTDCGFEGHADENAAVNI GRRFLERIDIEASSRT |
| 46 | cas12c | MTHAKKIPFPVLKRSTLRKARQRIAAGSITAGERPFNSTVTRVVPVKDP VSDQVWAVAREAAMTLRGFGQGSLFDMLIHLHADGFRLFPSGRERE AFFLKDLFDPTEFDDGARRAFGDVMPGFTANSLREILGAPARKCGKVT SVEILLPRLSKGLGVKKSAAPPEVLSSLAAALCEAFPTWSLLTAVDGGV GKVIDDVLRTHGSRLPSLEKAWSTNLPEVPKGLGVPTLAFDDQAPAQS EQTPTGRFAGVVARYLAETFASNPEATAGDASKAVQAKVTTPNGNAL SWLFAVGRRAMCSTTLDELAIGLNITSPRGRHALSSLKERMMALPALS VLGERAYPDSRATLQGTVDSLIANYVNRLFELSSSATSIAQTKLILPAAIQ GDTAVFDGMPFSAEDVGALFEQLPSEIAKLEHAVKVLVGKERTSTLGY QKAVDDVDEFGVWASSVDAVIGQINARLKTLERAQEPLGKLMGDGK LKRLVNIHEPEGPAVEIIPVLDQELQDVLTSCRTAFADLEARYPMTVAK AQRHAEAEVRNALELASRKEGGLSLASADVPALAKRKILEPIISIARRSS PAMATAVLTECLRQKLIVKGTGSERSLRGYVLSGEQVIYAHPLSRRRSI VRLDREGLQNFDALEFLDALQKDATQRTNVRESLIVEMARQSLLLSAL PDRIEIGAISWQTPSQNQHAPWANLRPVNGTVGRSETIKSFTAVFHS RISGLLYRLNRQKFMEKYDLRCFIGSTLLFSPKNADWAPPPQYRHGRF SALLARSDFPWEGAEGTHANAVRLAKFLIDETRNATDLQQAIAAKALL AQLPHDWVVCCDFDGAPSYEGAFVSAGEVSAWAKRSGYLLTPPRHF AGAFLEGFKSTKISPHGLTFERMLERDGDSVIETGRRVTAAFPITQEVA PAAQPWKPRHLAGLDLGEAGLGVCLKNLDNGHEQTLLLKTRKTRLLA HSAEHYRRKDQPRQVFRKQYNQSSENAIKAAIGEVCGLIDNLIARYDA VPVFESQAAAARGSNRMVARVYAGVLQRYTYVVGNGAADATRTSH WLGANRWSYSFGADVIPKVRDLSPEVLRSIKKPENVFRDALGFPGVLA NAWRTSMICSVCGTDPIGALEEAIAANQISFVTDNEGEGSLDLGDGRK VTLRVEVPTSSALTKREASRRKRRAPWEAKVGTVWTLTRKSHRDDLLT TIRRSLRRPSSTFQGSTTKQWEFHCPCCGQIQQADVNAASNLVRRYF VRASDNARARQHWADDSKRLAFIASMGPDRSAREEKVS |
| 47 | cas12d | MRKKLFKGYILHNKRLVYTGKAAIRSIKYPLVAPNKTALNNLSEKIIYDYE HLFGPLNVASYARNSNRYSLVDFWIDSLRAGVIWQSKSTSLIDLISKLE GSKSPSEKIFEQIDFELKNKLDKEQFKDIILLNTGIRSSSNVRSLRGRFLKC FKEEFRDTEEVIACVDKWSKDLIVEGKSILVSKQFLYWEEEFGIKIFPHFK DNHDLPKLTFFVEPSLEFSPHLPLANCLERLKKFDISRESLLGLDNNFSA FSNYFNELFNLLSRGEIKKIVTAVLAVSKSWENEPELEKRLHFLSEKAKLL GYPKLTSSWADYRMIIGGKIKSWHSNYTEQLIKVREDLKKHQIALDKL QEDLKKVVDSSLREQIEAQREALLPLLDTMLKEKDFSDDLELYRFILSDF KSLLNGSYQRYIQTEEERKEDRDVTKKYKDLYSNLRNIPRFFGESKKEQF NKFINKSLPTIDVGLKILEDIRNALETVSVRKPPSITEEYVTKQLEKLSRKY KINAFNSNRFKQITEQVLRKYNNGELPKISEVFYRYPRESHVAIRILPVKI SNPRKDISYLLDKYQISPDWKNSNPGEVVDLIEIYKLTLGWLLSCNKDFS MDFSSYDLKLFPEAASLIKNFGSCLSGYYLSKMIFNCITSEIKGMITLYTR DKFVVRYVTQMIGSNQKFPLLCLVGEKQTKNFSRNWGVLIEEKGDLG EEKNQEKCLIFKDKTDFAKAKEVEIFKNNIWRIRTSKYQIQFLNRLFKKT KEWDLMNLVLSEPSLVLEEEWGVSWDKDKLLPLLKKEKSCEERLYYSL PLNLVPATDYKEQSAEIEQRNTYLGLDVGEFGVAYAVVRIVRDRIELLS WGFLKDPALRKIRERVQDMKKKQVMAVFSSSSTAVARVREMAIHSL RNQIHSIALAYKAKIIYEISISNFETGGNRMAKIYRSIKVSDVYRESGADT LVSEMIWGKKNKQMGNHISSYATSYTCCNCARTPFELVIDNDKEYEK GGDEFIFNVGDEKKVRGFLQKSLLGKTIKGKEVLKSIKEYARPPIREVLLE GEDVEQLLKRRGNSYIYRCPFCGYKTDADIQAALNIACRGYISDNAKD AVKEGERKLDYILEVRKLWEKNGAVLRSAKFL |
| 48 | cas13a | MPIVKKFGRSQTSLSDRKIVLKMETAARNIPDFLLSDPEAVIGQWASA MDKIAKKPKGKDKPSSYQRKFRERLGKAIWADLTGPEGPLRDVPAAEL EDLRKRWDRRVHPYPDGTKDGPKPATPKGRLYTRFAGEVGYGKADA VAIARDIRIHLLETEFKTGGGTRDAGRAVRRASSIEKNVLKKARVPQRP KPPQEAAWSKEDEDRYFIPHDVARKIVLAAKAQEKEDHRVAWRTAA AVLFEHFGRIFQQDGRALSFAEAEKQMPGLLALHRAVEGYYRQALKR HRKDRREHEARPGREKGTGRRKVSAILPKDKTALLALIGHQHQNREIA ALIRLGRILHYEAGRRGNSDMVANINRNWPADVSESHYWTSAGQIEI KRNEAFVRIWRSALSHANRTLGDWLSPDEVANDITMSWESKHEKSG KRKTGKLEENREEAEAHAPVIFGGSAERLGTGDDFQKTLEAICEVFSQL RHSSFHFRGLDGFKDALTKTVKTCDPGAVARLQDLHAEDQANREARL KEDLRGAHAELFLDEGRLAEIWALLHPKSTEKTLPPLPRYSRVVTRAEN TCNGLKLPKSVNRESMKVPAIHCRYILTRLLYQSGFRTWIAEAPAAQLN RWIETATERAQKATVGITKNEADRARMVGQIKVPEGQGIRRFLDDLA GLTATEFRVQAGYESDREAARDQAAFLENLNCDVMALAFDKYLSDHK LGWLAGIDAESRPSETPLSNVDELPSSGSLGTPERWEAALYAVCHLIPV SEVGRLLHQLRRWSNGQKATPDGGRLERLFELYLDMHDAKFDGSTPL RDHDDLAVIFETTGIRDRVLPSSLQHGEHERLPLRGLREMLRFGNLRVL APIFATAKVDQAMIGELEGLEARIGDAPSQVDRAQALRTEMHAALCK KRKLAHDDKKSVKDYLTSLQTVIRHRRLANHVRLTNHVRTNEILMSV MGRLADFSGIWERDLYFVTNALLYQAGLTPCDVFSKEPPKENRRSPLQ EFENGQIVFALRKMQAQCDTHAGLVDQIKGETARLFHIAEGAPGNDP RIQNRNWFAHFNALKPKTGDRLDLTADMNRARDLMAYDRKLKNAV VSAIVTLLERENIVIAWTMKDHQLTDAVLAAKSIEHLKQNKIRENLRDE RSLGYVAALFGGRVAEEAPDIMHDRTVFHLVGALTEEMEPAE |
| 49 | cas13a | MRIVRPYGESRTDLGGERGQTRVLVDNTAARARHEIPDFAQSHDALV IAQWISVLDRIATKPQGTQGATRAQHAFRDRLGRAAWAQMCAADRI SAAAQADPYVAALWRFKTHPYGDAKYRPRKGKDGKPLGEPKPQGR WYGRFAANAEPEQADVAAIAALMDHHLHVAELRIDPKRPEKRKGLIE ARAKSIEGNVLVAEPRKRPVGSWSREAITRYFMRQDVAAEIFAAARD REQGLNDVPRGPVRLALAAKILHGHWTRLFHAPGTRTAYSIREAEEKE PELFALHMAVKDAYAKLLKRRTQPKTLKKGVKPPQQAPVTTVLPKNA GELLRLVQHRSRNRDLSALIRRGKLIHYTAFDIAAAAAEAESKTPDVPD ADRLAYVLTHWPDDLSASRYLTSDGQSAIKRSEAFVRVWRHTIAMAS LTLRDWASMNNDLGDVLGSANKVDQAIGRANFDPAWHDKKVRLLF GARAALFPSDDDGRKALLASVIRAGLALRNSSFHFTGRGGFLAALKKL GSEEVMVPSILAAAHALWREDATARAGRLRAALTGAHAAHYFEEDQ NASILTLLDEAPPKESLPIPRFRRVLGRAENTWKGKEALVLPPTANRRQ LEDPARRCRYTILKALYERPFRSWLIARAPEEVNAWIDRAIERTTRAAK DMNAKRGEDDKRSVIAAKAESLPRLSGERGIGDFFFDLSSATASEMRV QRGYGHDGEAAKEQAGYIDDLLCDVVALAFDAWLRNPQANGRPLTF ICDLKPETPLPAAPKCTLQEIGSAAEPVRPEDWQAALYLLLHLVPVGEA GRLLHQLAKWTVTSRLADDLLNANVTDDPSKAERTADEEDLKRLVHT LIQHLDMHDAKFEGGDALTGCEPFAALFASRPGFARIFPAEADERLDR RVPKRGLREIMRFGHHGLVASFAEDTRITDKEVGDYLRLEIEERPDNVA ALQARKEEAHERWVKAKEKRKTVDPKHLEDYVTALCGIARHRRLASR VTLTDQVQVHRLLMTVLGRLVDFSGMFERDLYFAMLGLLDEKGARP DEVFSGPIDEPKSRLALLANGRVLAALREQIPHSKDLAEELRKDLERLFG MDCSGIRLLEADERGDTCLRDIRNDLSHFNLLHDDSFALDLTTLVNRTR GLMSYDRKLKNAVSKSIKELLAREGLTLSWDMTDRHDLENARIGAKP AVHLGGRKLAFRGGDRRPEPVRENLHSPTHLEAVARLFGGKVVEEDD VTNLDLSSIDWAAEPHNSKETHRHRPAGPRKSPPKRRAYHAPR |
| 50 | cas13a | MRIIKPYGRTLVEHDGAGERKRVLTLRPDHDSKLDIEAFARDHDELVV AQWVSTIDKIAAKPGPRKGATEEQRAFRDRIGKAAWALLVRNALLPG LADADRADRLAKIWRRKIAPYGDLRPNERPASAKGRWYGAFAGEAD VADVDAGEIAAKIHEHLYDAEYRISGDGRKPDGCIAARARSIAVNVLRP ADSSACGQPEWSDRDLQAYRVADVAKQIWDAALSRENGRDGAGTK RVTNSVAGGVLFEHWARIFPGPDGKALSIREAIEKEPGLFALHMAVKD CYARILKHHKKKAPGRRERENGDVSPIRKVLPRDMDELFARIISGRGNR DLNALVRLGKVIHYTASDPNADHPESITENWPGDLAGSHYWTSAGQ AEIKRNEAFVRVWRHVVVLAARTLTDWGDPHGEIGSDILGKANDAT GAKFDEAAFNRKCALLFGKRASHFTAAPDLAFKKAVLKTAIKGMAALR HKSFHFAGRGGFVKALEGIGGLNEIDRFPDVTRALRTLLVEDIEDQSRQ VRATMVGAHFGVYLSKGQVEAIYRAVTGAEPGSLPLPRFSRVLRRAKG AWEAEDVLPPPVNRLDLEQRGRLCQYTGLKLLYERPFRRWLEGRSAA KLNGFIYRAVTRASDAARTLNTKESDDWRDIIVARAEKLGKVPDGGDI HGFFFELSAETASEMRVQQAYESDGERARQQAEYIEDLKCDVVGLAY RSFLETEGFDFLRTLDPEAAIAEAHRFDPAELPDPAVDTDAEDWEAVL YFLVHLVPVDEIGRLLHQMRKWDLLAHDRTAPVADGGQARLVDKVQ RVFTLYLDLHDAKFEGGEALTGIEPFRKLFEESDGFDTIFPPQQGYEEDR RVPLRGLREIMRFGDLPPLLSIYGRRPATKSNIERYRRAEVADAGGRSEI ARLQARREELHAKWVEAKKEGLGPEDRRAYVEALAEIVRHRHLAAHV TLTNHVRLHRLMMAVLGRLADFSGLWERDLYFATLALLHRAGKTPRE VFENEGIDLLRNGQIVYALRKLNGSSNASALRSGLFPHFGSAFKRGDPI GGIRNAFAHFNMLRAAQPPNLTECINRARQLMKHDRKLKNAVSKSVI DLLAREGLNIAWAVHTRAGAHDLAEAVLSSRQAQHLGKLRLFPVSGD GRDGKGFFIMEDLHGADFVEMAAELFGGRVSDRWRGKGCVSELRLD SIDWSRQREQKKHGGGKKPTGRARKANRGHKNRHRRA |
| 51 | cas12f | MSARNIKVKIDTKGNPELRLGLWKTHQVTNEGVKYYTEWLIKLRQQD IYRQSREDASPRVIISASDLKADLLCHARQLQKERLPRITGSDAEILGTLR QVYELIVPSSVGKSGDSKTLARKFLSPLTDPGSAGGRDQSASGRKPTW MKMKSEGNPRWEETFRKWKDRKDNDPTPLVLNQIADYGLLPLIPLFT DVGENIFDPKSKSQFVRTWDRSMFQQAIERLMSWESWNQRVRRE WEALNQKHSAFYREQFTADPDAALYRVAQSLEEEMRKEHQGFASDA PEAFRIRRVALKGFDRLLERWQKTLGKNGQSATLLDDIRRVQSDLGDK FGSAPLYQKLLDERWQRLWAVDPTFLQRYAAFNDLTQRLQRAKRVA NLTLPDAVAHPIWSRYEGANASSGNRYHIHLPTKGQPGSVTFDRILW PDGNGGWYERKRVTVFLRPSHQVDRIHEAPTDSVVDNFPLVVEDQS ARTILRASWGGAKLEYDRNRLPRQLKKGVPDSIYLSLTLNLDTNKPSGL FHTQQNGRVWIRKDVLMQYYNETPGDNVQFKPLYVMSVDLGIRSAA AVSIFSVQLKAGIEEHRLTYPVADCPGLVAVHERSVLLTMPGERREQW DRRYEQQRQGLRELRTDMRGMNDLLRGAYMDGDRREEFLARLSKLE ETSPELWGPVYRSLNDSKVASATEWERLVVYCHRQVEQSLSSRIQNLR SGRSAYRMSGGLSLDHVQDLERIRGIIASWTNHPRIPGSVVRWQQGR SHTVALGRHILELKRDRVKKVANYLIMTTLGYAYDSKRARGEKWVRRY PACHLMVFEDLTRYRFRTDRPRSENRQLMRWTHQELIAVTGIQAEPH GISVGTMYAGFSSRFDAVTKAPGVRGATVRQILRTRGMVRLKEIAAD VGIDINTLRPHDVLPTGDGEYLLSVVRHGESYRLKQVHADINAAHNLQ RRLWTQDEVFRVSCRLALNSGRVVAMPPPSYNKRYGKGFFEKGDNG VYIWKTGGKIKISDTLEEDMDIPEDTAELLRGNSVTLFRDPSGTIAGGN WLEAKEFWGRVNSLVNKGVRDKILGGIPVDNSSAHAE |
| 52 | cas12f | MPMIKITECVTWGTTCDGLWDARPHLEVRRSWSPPVQGGRTNRLD APPASVTLNIHGRVEHPRDADALSVAPLRVRHMFERTTTKAAFLSPLD LRPTQATDLERFAGTTRWAFNWANALLEAHHQAYEGRRQQAARHL FGLGPEQLDELRVLANGTRDENGKKAKGDPVKRREYESIQKATKKAVS EENKALGAEMKLWDEHRSLVVHKGRPLLTPGDEPALDAPPLAHRLYA RRVELAGIQKTDPDYYAEQRKKEREAITPNVVAMKRDLMAKGAYFPS EYDLQYIWRTVRDLPKEEGGSPWWPECPTILFYDGINRARTAWKNW MDSASGARKGPPVGMPRFKSKYKAKDTFTITNPNRSVIKFETYRRIAIT GIGSMRLHRGAKLLARRIAAGQAEITSATISRSGTAWYVSVLCTVHTTA RTAPSKAQRSRGAVGVDWGVRALATTSKPIALTPGKPASRTVPAEKY GAAMSQKIARAQRQLARMPKGSSRRRKAARHVADLQHLVAQRRAS SVHQLSKALAQSFEIVAIEGLNVRGMTKSAKGTVENPGKNIRQKAGLN RAILDATPGELKRQLEYKTKKYGSRLVELDTWYPSSKTCSRCGWVHPK LKLSMRTFRCQQCGLVEDRDFNAAVNIERQGITHIVKENEGTDDREE G |
| 53 | cas12f | MSTPMGWTAVNGGDATSPTTRVSSPPGEPRTGACPRAAAADATRA ESSPRRTSSPARPGERHARARTSRYPIPNTYVVDRPSAEGDRHGQSSL DCGPCPVRRSGALHQSSQAAHRRSMTGAKQKTPIRVVRFSIDHSALT PAQVVAFARHAGAARQTWNWALGRWMDWRNNTKFYVDYKVFKA AGMGPGLSTDDLIQVIERAVSIRQDDKWMDAAWDEARQIHGEWD QFQKASTLQSLYLAGAQEPFDPSRDDGINPYHWWVTEGDKSGLPKA ERHNVNSGATYTAPLRAFEEAVGRFYKLPGKKGTPKFKSKHDDEQGF CIQRLTETGLSPWRAIEGGHRIKVPSIGSIRVVQSTKRLRQLIKRGGKTT SARFTRRGGKWFVSVSVAFDLSAPRVQRPARLSRRQRAGGSTGVDLG VNRLATLSSGDQFPNRRLLRKSMAEIKRLQRKFDRQHRAGSPECFNE DGTHKKRCRWGREDGPAMSRSAQTTKRQLRRIHDLTARRRAGVLHE ITKDLATRFELIGVEDLNVAGMTAKSKPKPDPDRPGHFLPNRRAAKAG LNRAILDVGFYEFKRQLGYKTEWYGSTMQMVHRYAATSKTCSGCGW VKPKLTLAERTFNCTQCGLAMDRDHNAAVNIRALALEGAAPMEREQ PAPVGAAEKRHRDPVSHRRRPKSLAPCESTRPVRDLSPPATQEETA |
| 54 | cas12f | MAQAEAPRRLRAYKFALDPTEAQLREFEQHAGSARWAYNHANAILS RYSDTLRNRWNAWIAQHHGLSREQLYALPDRERTAIQAAARAAVKA ENAQLAAELRIIDDHRKRVTHKGKPSVEPGEQPAEDAPERAYQLWRE RVELARLHAEDPQAYRAERKRILDEIRPLVNATKRKLIEQGAYRPTAM DISTLWREIRDLPPDEGGSPWWPEVSIYAFTSGFAHAETAWKNYLESL AGRRAGRPVGKPRFKKKRRSRRSFTLYGSVKLVTYRRIQVPSIGSVRLH GSAKRLHRALERRGGIIKSITISQGGHRWYASVLVDELDITPGRETQRG PSRRQRDRGAVGVDLGVHHLVALSDPNEKTLDNPRHLRKARKRLLKA QRAMSRRRGPDKRTGQEPSRRWVKARNRVARLHHELAVRRAGHLH EITKRLATSYELVAIEDLNVAGMTRSARGTIDQPGRGVRAKAGLNRSIL DTSPAEFRRQLQYKASWYGATVAVIDRWAPTSRTCSSCGAVKAKLSL AERTFFCEHCGMELDRDINAARNILAFAQSAYPGEGKALNACGGSVS PGSQSVVQAGADEAGRPARKPRRSSRGSDPPATPTTRA |
| 55 | cas12a | MTSSSPTQRAYTLRLKSAAQGDKSWAEKLWDTHEIVNKGARAFGDW LLTLRGGISHKLENLNDKETGEEGKKRRRILLALSWLSVESKDFAPEKYI VEKDGEDKHRTKEALEAILKSRNLEDEEVESWVNDCKDSLTSSIRDDA VWVNRSRAFDDAVRKIGDSLTREEIWDVLGRFFGKKEAYLAPRTIDEK NGKTKKEEPKDLARKAGGWLSKRFGKGKGTDFSKLSKVYSEIVKWAEE PRKSEPRTLANLASALKEDSLQGILNLIKNSGSKSGTRNFLEEIGEGEVS KENLAILKAKAEGNRNYCKKEIGGKGRREWSDRILKSIEETLDGKFTYL QEKGPARHWEFAVMLDHAARRISAGHTWIKLAEARRRNFEEDSQKI NEVPENARQWLETYREDRSKSSGAIEGYLISKRAVTEWETVVKAWKN CKTEEDRIAAAGALQDNLGIDQFGDINLFRALASEDVRCVWQVDGKP DANILLNYVAATKAEFDKRRFKVPAYRHPDPLLHPVFCDYGNSRWEIR FDVHEVNRTGKKAKQNKKTIETADVHGLKMDLWTGSKIENVSLRWQ SKLLEKDLAVKQLDGKEDGKKEVSRASRLGRAAVGAGWETPVSASSV FAQKHWNGRLQASRKELSRIARRVKTRGWDEKANSMKKNLKWFITF SPKLKLQGPWISYVDNSEDKRPFTFTSKGEPILDEVFSIENKNRKGRAR LILSRLPGLRVLSMDLGHRHAAACAVWETLSSRQLEDACAEGGYDKP APDAMYHHIKSNRGKRVIYRRIGADELSDDSIHPTPWARLERQFLIKLQ GEERKARMATADEIWEVHELERALGRKTPLVDRLTKSGWGSDSGTPR QRQLLGELNQWGWEPDEAQENSEDDEITSRESLLVDKLMSRTVDTV RKGLRRHGNRARIANFLVAREKTVPGGQMDTLNNEGRKEIIADALAF WYELANGGEWKDTEALDWWKIHIEPELSVEELPDIAGTGIAPKERKR KKKELKEKLKPVAERLLTSGAKKLSDQWCERWKQDDKEWQKTLRWL RDWILPRGVRGKSELIRNVGGLSLDRLTTIQSLYQAQKAYFTRITPKGIQ MDKDKPLTAVMNFGGHILNDLENMREQRVKQLASRIVEAALGVGRV KIPKKSKDPKRHYERVDAPCHAVVIENLTNYRPEETRTRRENRQLMT WCSGKVKKYLSESCSLHGLFLWEVPPSYTSRQDSRTGSPGIRCEEVSVE KFFKTPFRQREVARAEEKDSKNKASAYEQYLIDLKERWKSRGEETALLR IPRKGGEIFVSANSNSPASKGLQADLNAAANIGLKAITDPDWSGSWW YVPCSSKDFVPIKDKIGGSRAFENITTPMPNPDDAKEATGKKRSGKKEII NLWRNPACSPLERDEWERTAKYWNMVEYHVIKRLKRQMG |
| 56 | cas12a | MKNFQDFTNLYELSKTLRFELKPIWGTKKLIEEKNILKLDKKKRENYEKV KPYFNKIHQEFINFALRNPNFDFSQFEEKYLNWLKDKKNKDLLKEKESI DKIFLEKIWKLFENSVKDFLKENGFESIVKSEDQNLKFFRRKEIFEVLQEK YGSELETQMVNKDWEIKSIFNGWEKWLWYFDKFFNTRDNFYKTDW TSTAIATRIIKDNLKIFLENTIIFEKVKNKKIDFSEVEKNFSVSIDTFFEINNF NNCFLQDWIDFYNKVIWGETLENWEKLKWLNEIINKYRQDTGEKIPY FKKLQKQILSEKDWVFIDKIEDDGGFYEVLKNFYKNAAEKEWFLKNIFE NFYTISDKNLEKIYFNKIAFNTISHKFWSALEFERILYEEMKKEKADWIKF EKKENKYKFPDFIQIIFIKRSLENYDSENLFWKERYYKSEENVDWFLEKN NNNIWEQFCKILNFEFLNILKRRIIDEAWEEYEVWFEISKNILWEKLENF ELNQENKWIIKDFADYSLALYSFWKYFAVEKWRNWDLNIDISDDFYG WEDWYIEKFYNTGYDEIVKPYNLMRNYISKKPWEDSKKWKINFETSSL LSWWDKNLESNWSYIFQKWNKYYIWIINWSKPAKEVLEKLYSWNGE KIKRFIYDFQKPDNKNTPRMFIRSKKDSFSPAVGKYNLPVEDILEIYDN WLFKTENKDNSNYKESLSKLIDYFKLGFSKHESFKHFNFVWKDSKEYEN IADFYRDVEKSCYQITSEFLDFEELKKLTFKKHLYLFQIYNKDFELDESLQ KNWYNFRDEWQKNIHTKYFEALFLEENILRKSWAVFKLSWGWEVFF RKESIKAEKEKRKNIEVTKNRRYTEEKYFLHFPIQVNFKNEISWNFNQEI NKFLANNPDINVIWIDRWEKHLAYFSVINQKWEILESWSFNKIENYNK NWEKLLFPEREIKEIHKDWSLIDLELVETWRKVDYVDYKLLLEYKERKRL LQRQSWKEVEQIKDLKKWYISALVRKIADLIIKHNAIVIFEDLNFRFKQI RGWIEKSIYQQLEKALIDKLNFLVNKNEINLEKAGSILKAYQLTVPVDSL KEIWKQTWVIFYTEAAYTSKIDPIKWWRPNLYLKKQNAEINKENILKFD NIIFNSKENRFEFTYDLKKFFWKDSKFPAKTVNTVCSCVERFKWNRNL NNNKWGYIHYENLTDWKLANKEQKEDEFSNFKELFEKYFIDINWNILE QIKNLDTKNNEKFFSSFIDLFTLVCQIRNTNQNAKWDENDFILSPVEPF FDSRKSQNFWKSLPKNWDENWAFNIARKGLIILNRISENPEKPDLLIFN ADWDNFARNI |
| 57 | cas13b | MTEQNEKPYNGTYYTLEDKHFWAAFFNLARHNAYITLAHIDRQLAYS KADITNDEDILFFKGQWKNLDNDLERKARLRSLILKHFSFLEGAAYGKK LFESQSSGNKSSKKKELTKKEKEELQANALSLDNLKSILFDFLQKLKDFR NYYSHYRHPESSELPLFDGNMLQRLYNVFDVSVQRVKRDHEHNDKV DPHRHFNHLVRKGKKDKYGNNDNPFFKHHFVDREEKVTEAGLLFFVS LFLEKRDAIWMQKKIRGFKGGTEAYQQMTNEVFCRSRISLPKLKLESL RTDDWMLLDMLNELVRCPKSLYDRLREEDRARFRVPVDILSDEDDTD GTEEDPFKNTLVRHQDRFPYFALRYFDLKKVFTSLRFHIDLGTYHFAIYK KNIGEQPEDRHLTRNLYGFGRIQDFAEEHRPEEWKRLVRDLDYFETGD KPYITQTTPHYHIEKGKIGLRFVPEGQLLWPSPEVGATRTGRSKYAQDK RFTAEAFLSVHELMPMMFYYFLLREKYSEEASAEKVQGRIKRVIEDVYA VYDAFARDEINTRDELDACLADKGIRRGHLPRQMIAILSQEHKDMEEK VRKKLQEMIADTDHRLDMLDRQTDRKIRIGRKNAGLPKSGVIADWLV RDMMRFQPVAKDTSGKPLNNSKANSTEYRMLQRALALFGGEKERLT PYFRQMNLTGGNNPHPFLHETRWESHTNILSFYRSYLKARKAFLQSIG RSDREENHRFLLLKEPKTDRQTLVAGWKSEFHLPRGIFTEAVRDCLIEM GYDEVGSYKEVGFMAKAVPLYFERACKDRVQPFYDYPFNVGNSLKPK KGRFLSKEKRAEEWESGKERFRDLEAWSHSAARRIEDAFVGIEYASWE NKKKIEQLLQDLSLWETFESKLKVKADKINIAKLKKEILEAKEHPYHDFKS WQKFERELRLVKNQDIITWMMCRDLMEENKVEGLDTGTLYLKDIRT DVQEQGSLNVLNHVKPMRLPVVVYRADSRGHVHKEEAPLATVYIEER DTKLLKQGNFKSFVKDRRLNGLFSFVDTGALAMEQYPISKLRVEYELAK YQTARVCAFEQTLELEESLLTRYPHLPDESFREMLESWSDPLLDKWPD LQREVRLLIAVRNAFSHNQYPMYDETIFSSIRKYDPSSLDAIEERMGLNI AHRLSEEVKLAKEMVERIIQA |
| 58 | cas13b | MESIKNSQKSTGKTLQKDPPYFGLYLNMALLNVRKVENHIRKWLGDV ALLPEKSGFHSLLTTDNLSSAKWTRFYYKSRKFLPFLEMFDSDKKSYEN RRETTECLDTIDRQKISSLLKEVYGKLQDIRNAFSHYHIDDQSVKHTALII SSEMHRFIENAYSFALQKTRARFTGVFVETDFLQAEEKGDNKKFFAIG GNEGIKLKDNALIFLICLFLDREEAFKFLSRATGFKSTKEKGFLAVRETFC ALCCRQPHERLLSVNPREALLMDMLNELNRCPDILFEMLDEKDQKSFL PLLGEEEQAHILENSLNDELCEAIDDPFEMIASLSKRVRYKNRFPYLML RYIEEKNLLPFIRFRIDLGCLELASYPKKMGEENNYERSVTDHAMAFGR LTDFHNEDAVLQQITKGITDEVRFSLYAPRYAIYNNKIGFVRTGGSDKIS FPTLKKKGGEGHCVAYTLQNTKSFGFISIYDLRKILLLSFLDKDKAKNIVS GLLEQCEKHWKDLSENLFDAIRTELQKEFPVPLIRYTLPRSKGGKLVSSK LADKQEKYESEFERRKEKLTEILSEKDFDLSQIPRRMIDEWLNVLPTSRE KKLKGYVETLKLDCRERLRVFEKREKGEHPVPPRIGEMATDLAKDIIRM VIDQGVKQRITSAYYSEIQRCLAQYAGDDNRRHLDSIIRELRLKDTKNG HPFLGKVLRPGLGHTEKLYQRYFEEKKEWLEATFYPAASPKRVPRFVN PPTGKQKELPLIIRNLMKERPEWRDWKQRKNSHPIDLPSQLFENEICR LLKDKIGKEPSGKLKWNEMFKLYWDKEFPNGMQRFYRCKRRVEVFD KVVEYEYSEEGGNYKKYYEALIDEVVRQKISSSKEKSKLQVEDLTLSVRR VFKRAINEKEYQLRLLCEDDRLLFMAVRDLYDWKEAQLDLDKIDNML GEPVSVSQVIQLEGGQPDAVIKAECKLKDVSKLMRYCYDGRVKGLMP YFANHEATQEQVEMELRHYEDHRRRVFNWVFALEKSVLKNEKLRRFY EESQGGCEHRRCIDALRKASLVSEEEYEFLVHIRNKSAHNQFPDLEIGKL PPNVTSGFCECIWSKYKAIICRIIPFIDPERRFFGKLLEQK |
| 59 | cas13b | MESIKNSQKSTGKTLQKDPPYFGLYLNMALLNVRKVENHIRKWLGDV ALLPEKSGFHSLLTTDNLSSAKWTRFYYKSRKFLPFLEMFDSDKKSYEN RRETAECLDTIDRQKISSLLKEVYGKLQDIRNAFSHYHIDDQSVKHTALII SSEMHRFIENAYSFALQKTRARFTGVFVETDFLQAEEKGDNKKFFAIG GNEGIKLKDNALIFLICLFLDREEAFKFLSRATGFKSTKEKGFLAVRETFC ALCCRQPHERLLSVNPREALLMDMLNELNRCPDILFEMLDEKDQKSFL PLLGEEEQAHILENSLNDELCEAIDDPFEMIASLSKRVRYKNRFPYLML RYIEEKNLLPFIRFRIDLGCLELASYPKKMGEENNYERSVTDHAMAFGR LTDFHNEDAVLQQITKGITDEVRFSLYAPRYAIYNNKIGFVRTSGSDKIS FPTLKKKGGEGHCVAYTLQNTKSFGFISIYDLRKILLLSFLDKDKAKNIVS GLLEQCEKHWKDLSENLFDAIRTELQKEFPVPLIRYTLPRSKGGKLVSSK LADKQEKYESEFERRKEKLTEILSEKDFDLSQIPRRMIDEWLNVLPTSRE KKLKGYVETLKLDCRERLRVFEKREKGEHPLPPRIGEMATDLAKDIIRM VIDQGVKQRITSAYYSEIQRCLAQYAGDDNRRHLDSIIRELRLKDTKNG HPFLGKVLRPGLGHTEKLYQRYFEEKKEWLEATFYPAASPKRVPRFVN PPTGKQKELPLIIRNLMKERPEWRDWKQRKNSHPIDLPSQLFENEICR LLKDKIGKEPSGKLKWNEMFKLYWDKEFPNGMQRFYRCKRRVEVFD KVVEYEYSEEGGNYKKYYEALIDEVVRQKISSSKEKSKLQVEDLTLSVRR VFKRAINEKEYQLRLLCEDDRLLFMAVRDLYDWKEAQLDLDKIDNML GEPVSVSQVIQLEGGQPDAVIKAECKLKDVSKLMRYCYDGRVKGLMP YFANHEATQEQVEMELRHYEDHRRRVFNWVFALEKSVLKNEKLRRFY EESQGGCEHRRCIDALRKASLVSEEEYEFLVHIRNKSAHNQFPDLEIGKL PPNVTSGFCECIWSKYKAIICRIIPFIDPERRFFGKLLEQK |
| 60 | cas13b | MDTPNFSERIPVSLQSHPYYFAHYLNMARHNAYVILEYVNRELIKPGK NLDEDNLIQSTVLKDGYFDRKPDELSHRNRLLVQHFPFLREAENEGAR TCNPVSYKLKTALAALNQWRNNASHYPLNQNHEKDFDLQPFFSFAIE ACKKRMREVFQPDDFYLLETNEKQFYTLHNENGFTEKGLYCFICFFLEK KYAFQFLAGIKGFKNTTDNKFRATLETFTEHCCRLPKPKLDSSDIKLDM LGELSRCPAPLFDLLDIEERKKFIREPEEVKPDESGDREEVQQVLMKRY DDRFPYFALRYFEEKNLLKGISFHIHIGRWIKSEHTKKIMGAERDRRLLK DIRTFGELKEFSPEHAPDYWLRDGITPDDVDQFSPQYRIVGNRIGIKLN YNGHNRWSVPDKEINVKPDAIISTYEFLNLFLYEHLYQKKLTGLSPAEFI QDYLDRFNNFLSEFKAGHIRPVGDFSLEKRRGQGDEPDLTARRKSLQK ELDRFVLKGKDLPDKIREYLLGYKQKSEKKQAKWILGGMIKETVYWRN KAEQSPEKMRSGDMAQQLARDIIFLTPPHTVKEHKQKLNSLEYDVLQ YALAYFSSNREKLYSFFKEHQLTVKGDRAHPFLYKIRLDECQGILDFFIVY MQQKEKWLGWLDRNLKSPRLNEEEFFNTYSYFIKTDTKRAIEMDYES CPNYLPRGIFNEPIAKALQKAGVKIKDEDNASYALSVYSNGKTQPFYNK ERYYNKGIFRMEELPEKLQPKELLGKIQWTIKSSGKDTEEFRSLQNLKN RILNTEKEIRYVQSTDRALWIMVADLFPETFELRPDDLECIGHDLSDDLL SRPYQMKEKVYNYTITDYLPIKRYGEFRRFLKDRRLENLLTYFEEGVPLH REALVAELEAYDLQRKNLLEIIYRFEKLVFDRHRHELTFSGEGENQYVN HWDYLDFVARKYGLSAEVKELNSERFTELRNKMLHNQIPYQLWIKEAI AAREENTVCGRIMGMIGEIYERMTTEIEKQMQV |
| 61 | cas13b | MFDNEQKNLEKEPYWGVFLNQARLNAYIALRDISERLEENAADEDSLS EWPVLKYLDNDTDAVKSRRIFDLVEKHFSMLKIIYGGEKEGDLVKRSKE YKIILKCLFRALNFYRNKFCHMYSGNRARKYNEKELIKYLEDCFDASVRK IKELRRLDEKDVLHLRRKIAEGKDANKRVIDNPQFRYPFKNEKGELNEK GLYFLASIFLDKKEAHEFLKKQEYFKNDSEPKYRATLESFYHYRIKLPRPV IESDVDKNGLALDMLNELKKCPKELFDLLSKEQQEKFRVVDSEDADEE GNEILMRRYSDRFPYLALRYCDENQVFERIRFQIDLGRYYFKFYPKETID GKTQQRSLDKRLKIFGRIKDVKSKVEQEWSGIIKSPDTIEENPNEPYKLK TTPRYNIVDNQIGFVITGDKNLPDVKRPDGRIELEKPDGWLSIYELPGM LFHGLKYGFDKTERMIKIYIEKQRKICKEICEKGTITPDDGESMPEALKG GAKAAKRNYSEKKLERMLQDTEQRIRAIQTTQKRMDEPGNKPGKKKF FDIRAGKLADFLARDIMALQRFDPAKHGKDKLTAINFQVLQATLAFYG AKKDVIEDMFKGIGLLEGDNPHPFLNQIDPAQYNSIAGFYQAYLQKKR SYLEDYRKEEEYDEQFLRPKRQRYAQEKREIKTVARQLLDNPVNVPKN FFKKEIEEFVFSQDPSLKKSKMNTAYMIQALFEKHYGRQQPFYSYNRT YPVVSKAIEYGKKGKNKKIAKVLMAIEPKLNYMEIKKIVNEMPDGQYE PENLKRNLYEGYKDYEKDERIIRRCKVQDVVSFMMVEETLKDQLDFN GNVLTLEKITPWEASPFKKPVLCHTIISIPFNTKGGHTDKDYVDFIKNNF EGSYDCEPNKIILKYKVTSKDTKLKDIGKYRMYSHDRRLPGLLIWKYRP NDQNGNEIKFTEIEQEIKAFERRRIEIAQCLYTLEKKVIDSWFTQDELGE EHIPFNKVIDVIKAKMPNFEDKCNVLLKIRNAINHNQFPVYEQAIQTAP GKEIAGKMLRITESYIEQIMAKIDPDFGRTEDAESSR |
| 62 | cas13b | MDTPNFSERIPVSLQSHPYYFAHYLNMARHNAYVILEYVNRELIKPGK NLDEDNLIQSTVLKDGYFDRKPDELSHRNRLLVQHFPFLREAENEGAR TCNPVSYKLKTALAALNQWRNNASHYPLNQNHEKDFDLQPFFSFAIE ACKKRMREVFQPDDFYLLETNEKQFYTLHNENGFTEKGLYCFICFFLEK KYAFQFLAGIKGFKNTTDNKFRATLETFTEHCCRLPKPKLDSSDIKLDM LGELSRCPAPLFDLLDIEERKKFIREPEEVKPDESGDREEVQQVLMKRY DDRFPYFALRYFEEKNLLKGISFHIHIGRWIKSEHTKKIMGAERDRRLLK DIRTFGELKEFSPEHAPDYWLRDGITPDDVDQFSXPQYRIVGNRIGIKL NYNGHNRWSVPDKEINVKPDAIISTYEFLNLFLYEHLYQKKLTGLSPAE FIQDYLDRFNNFLSEFKAGHIRPVGDFSLEKRRGQGDEPDLTARRKSL QKELDRFVLKGKDLPDKIREYLLGYKQKSEKKQAKWILGGMIKETVYW RNKAEQSPEKMRSGDMAQQLARDIIFLTPPHTVKEHKQKLNSLEYDV LQYALAYFSSNREKLYSFFKEHQLTVKGDRAHPFLYKIRLDECQGILDFFI VYMQQKEKWLGWLDRNLKSPRLNEEEFFNTYSYFIKTDTKRAIEMDY ESCPNYLPRGIFNEPIAKAVQKAGVKIKDEDNASYALSVYSNGKTQPFY NKERYYNKGIFRMEELPEKLQPKELLGKIQWTIKSSGKDTEEFRSLQNL KNRILNTEKEIRYVQSTDRALWIMVADLFPETFELRPDDLECIGHDLSD DLLSRPYQMKEKVYNYTITDYLPIKRYGEFRRFLKDRRLENLLTYFEEGV PLHREALVAELEAYDLQRKNLLEIIYRFEKLVFDRHRHELTFSGEGENQY VNHWDYLDFVARKYGLSAEVKELNSERFTELRNKMLHNQIPYQLWIK EAIAAREENTVCGRIMGMIGEIYERMTTEIEKQMQV |
| 63 | cas13b | MKTLGALSSHNYNNKKYYFSGLLNTAQYNFNLALQEVNDRLGKKGKN PGKTMIKNIFDQKDSFSTQERAMYYLEEFFPWIFLVMKQSGINIPTEE QETKLHKEEIQLIQEHLISLYELLDDLRNEQTHYMHDPVIIPEEVSKMLD ALLLQILKNTRKKCKDDEYRTFIVKKYQEEFQKEIKVQVKDRFGKEKEKI VTGEVKENYVINRCFRKWIQKEGEEETLRYSTVQEEQGKYVWSSSGFV FFLSLFLRRKELEDVMNHVPYFKDSRKLLFYLTRKTFSSYCFRDLRKSLRS DYSNDSLLMQMIEELYKCPGELYEVLLKEQKQEFIEDINEYYKDNPEFE GSANEAQVIHPVIRKRYQDKFPYFALRFIDEYFNFPTLRFQLVLGEYVT DRRTKELQGTALFTDRVISQRISYVGKLSEAEMNKKREGYTETGWKEY PNPYYKIENNRIPLYIEFSKNEELIFKEKKFKYNTLAKWENREIDKRTGEF NQVNKQRRITQLEEFKIDNPKKMKTPNVFLSIYELPALLHALLIEKKTEA EIEDIIKAKIKKQLTEIAEGRRNLSGLPKGIKKMRNCNSDFEKKKLISDID NEIKKGEKILEEVQQWLNPVINKKGTGKQENNKPFFSNTYRGKYATW LAYDIKRFTGKDHIQNWKGYQFSELQTLLSLYTLRKEELKNFLEKDLQLT SHPFLKEALKAVNLEDFMGAYLRGRQFFLEKAKKQIGIKGVKKSIFQYF EERKYKIYSSNLDYWEELWKHPVNLDRGLFDERGTVYNKNKELNDLQ NRAAWFSFAETNPKQQFYHFPRIYSDEDITKPVTDRYGKTKEKLILFKLS PQKGFMEQIPSDLKKKYQEDKGKVEHPEVQKEKKYEEKKHPGINAFIK NAYKNEQKIRRISRNDIFLYEMVKYMLNKISPATEFSSLDKVWLTRIERE KQATEAREQSFKEKGDTSENKIRQDYLLSFPITLTLFNDIIKEKVKIKDIG RFRKLEKDERVQTMISYYTSGLWKNDQPSLTIKELEAELESYHKIRLQEI FKEVHKLEKEIYEFTPEEDKSKLLARESFPKFKYYISFYFIPKEDQEVFNEI QFDKYKNLEQIPGRKPEYDPYYLLIFIRNKFAHNQLPAEPIYKTALTFLP NNFNTLAEYYHKLFILLNNKNYNN |
| 64 | cas13b | MNILPAAPEKEKIAYSTATAPWFFGAFLNQARHNLFLTVNDLAIRLGE KVIDYDDQLLNSNVVRMLVNEKASPLQLEILMKYLDRHLPFLIPMQVA LKGHQGDASDNPVIGSPADYGAILSKLIVCLNAARNHFSHYHSTSGWS GYNEVIEWMEHVFTRNIETVVKRFTLTEEEVQHLKKPVDKSPKGTIPPY YFSFCKGDIWTDTGLAFFICLFLTREEAYLFLKKLRGFKRGEERFHKATLE AFCVGSLKVPRERLESNNSPQSAFLDMCNELVRCPKSLFDLLEPEKQEL FRRDPEPEDAEDNGIEEEEDQPQALLVRKENRFSYFALRYLDIAKAFPR LRFGVDLGTYFFSVYPKTFAGIEETRQLSKRLIGYGKLEEFAREKRPEHIA ALFRSKEEANAAPTEPFIRETAPHYHLDGNNVYLYMSGDGEAQWPAV ELEEVTGKSYPRKLVKKSTLLPFAVLTVNELPALLFYHLLHKEKGAGDAA ERVIINHMERVKRFFKALQDDKVDQVAGQPIRKPDVDADESLHMEY DRRWKLLKKKLSEYQLRASYIPEKIINYLLNIEAVDLGDKAMAQLKNLQ RQAQDDIAAIERRMEHLMKKGADGRKTLKVGNLAQQLAEDMLQM QPVQIGTDGEPVPASKANNLAFRLLQSHLAYFAENRHNLPAVFEACGL IGASNKHPFLDNINIESCKGVVDFFILNFRNKLDFLDRCLQEGEWHRYH FISAAKLKSGAKVTIKKYLNEAFESKGRNHIPFTLPPSLFLDASLDWLAKF GDGKAKKVLAENEYVNSVFLIRRLFADGGLQPFYAWKREYRLFEKKAG KAVFLDEAGRMRKADKIGIEVERHREFLARPVKKGKQYDIKKAAAEQF LRSYRFYLQEEKYIRLLAAQDMLLFRCICDLLTYHVGDIGLEELAEAKAG TFSLANITPEKTETAKSLLNYRPAGGVVLDRHFYATDEKGAFVKQEGKL VPGGQVRIFDNTLKIKNAGNFRKLLKDRRMNNLFFYFKQHADEPVVL HRMVLENELRAYDRMRLKVLPVIAEFEKKLYQHCTDVEKERLVVNGS MHHRCYLDVYREKYQPDWGWEAAGNLLRIRNAFVHNQFPLMEGD GFKLEVAHWKKINADFVPSEQGSSLGYGIIDRLGQLAVEGYEGLIKNIH V |
| 65 | cas13b | MGEEEQAHILENSLNDELCEAIDDPFEMIASLSKRARYKDRFPYLMLRY IEEKNLLPFIRFRIDLGCLELASYPKKMGEENNYERSVTDHAMAFGRLT DFHNEDEVLQQITKGITDEVRFSLYAPRYAIYNNKIGFVWTSRSKKKSF PTLKKKEGEGHRVAYTLQNEESFGFISIYDLRKILLLSFLDEGKNIVSGLF KQSKANWENLSENLFDAIRTELQKEFPVPLIRYTLPRSKGGKFVDPKLA DKQEKYESEFERRKEKLSEILSEKGFDLSQIPRRMIDEWLNVLPTSKEKK LKGYVETLKLDCRERLRVFEKREKGEHPVPPRIGEMATDLAKDIIRMVI DQGMKQRITSAYYSEIQRCLAQYAGDDNRRHLDSIIRELGLKDRKKGH PFLGKVLRPDLDHTEKLYQRYFKEKKEWLEATFYPAANPKRVPRFVNP PAEKQKELPLIIHNLMKERPEWRDWKQRKNSHPIDLPSQLFENEICRLL KDKIGKESSGKLKWNEMFKLYWDKEFPNGMQRFYRCKRRVEVFDKV VEYEYSEEGGNYKKYYEALINEVVRQKISSSKENSKLQVEDLTLSVRRAF KRAINEKEYQLRLVCEDDRLLFMAVRDLYDWKEVQLDLNKIDNMLGE PVSVSQVIQLENGQPDAVIKAECKLKDVSKLMRYCYDGRVKGLMPYF ANHEATQEQVEVELRHYEDHRRRVFDWVFALEKSVLKNEKLRRLYEK SQEGCEHRRCIDALRKATLVSEEEYKFLVHIRNKSAHNQFPDLEFGKLT PNVTSGFCECIWSKYKAIICRIIPFIDPERRFFGKLLEQK |
| 66 | cas13b | MRIPKLIEEHKSVFGAYSTMALSNVETVLNHIAERAGLDGYERDRGPG VEDYWEHPVMQCLCRKDKPRSIPSDVLLDVRNRLFRSFPFLKIMAEN QRDYRNAKGKVECVEINESDIFVVLNNSFRVLKAYRDTCTHYLIENRIW EDNSPMLMYNECPLAAMVNQYYTAALRVTKERYGYETRDLTFIQKRR FKQEPEKEASGNVKKKAVPDLAFFLSLVALNGDGRKWLHLSGWGVVL LICLFLEKKYVNVFLSKLPNPGNYPPSSKERRIIRRSMGVCSVVLPKERIH SETGDLSVALDMLNELKRCPRELFDTLSPGDQERFRTISSDHNEVLQM RSKDRFAQLVLQYIDHNRLFENLRFHVNMGKLRYLFNPKKYCIDGQTR VRVLEHPLNGFGRLQEMEEKRLQENGPFARSGIKVRCFDEVRRDDAN ESNYPYIVDTYTHYVLENDMVEMFFCPEGSGMKMPEVTSREGKWYV DKKVPHCRMRMSVLELPAMLFHLLLCGAKNTEVHIGKVCDNYCHLFS DMAQGNLTEENILSYGIKKEDIPQKVWDCVRGVHMGKDSRAYREKEI RERYEDVTRRLERLEADRKAVLGGENKIGKRGFVQIVPGRLAAYLATDI CRLQPSLRKGDGYGTDRLTGLNFRLLQSSIATYNCGESDILYGRFRDVF CSAGLIGGDNPHPFLDKVLPEAYSVCCPRNTIEFYERYLEEYQRYLKPLV IKLEKGKVPSLSFVNEGQRRWARRDDAYYHELGNLYLSQAIELPRQMF DDEIKDKLREMPEMRDVDFDHANVTFLIGEYLKRVRHDESQEFYSWP RHYKYVDMLKCILNPKNGSLQAVYIQMGEREGLWQERSELEEKYAKIR LRDLGRKGLDKDEANERIKTGLGNRKKEYQKAEKVIRRYKVQDALLFM LAKNTLFNSVEVDDERFKLKDIMPDGEKGILSEVVPMDFCFRSGNSAT RKLMGTIHSDNTKIKNYGDFFALANDKRMVTLLPLVGEQCLVKEEVKE EFDKYDDCRPEMISMVFDFEQWAYSAYPELKELVSNEAIKGRLFSNLL QELLGRGELTYEEKYALVGIRNAFLHNSYPKDGGVVKVRTLPDIAKSLK DVFKEYIRLE |
| 67 | cas13b | MKMFYKSVLTAFFTAVDSLRNKYTHYSHKDLNIREIKIECTLGGKDYCI GLLNALDCIYDSAVNLLKLRFMAGEDEVAHLRRCKAVNKKVVVRTEKD GFYYRLSDNGGVTEKGVIFIASMFLNRKYGFLFLKQLEGFKRSDEKRYR LTLEAFLAFSNIKPVDRLKSDKLDRASLGLDMLNELTKIPKELSETLSVDC LYKYLASDGEDDLRSRIRYQDRFVPLALEFISQSDEFKDFRFYTYVGNYV YKGYIKRLIDGTDKERYLSDRLCGFYKSVNDASSDAIAQKYGVEIKDSNE PDYMLPDSFRPHVLRATPHFVINTNNIGIKICGNDCLPIVNGKGVESPE PDYWLSIYELPAMLFYAYLREKNGKRFKDYKSIRELIEGVEKKADEKND RDKGALMARHIDKEIIWTQTKLDEVKRLEEKKVAAYGKKGRVVLKAGR MADLLAHDMVRLQPATKGSDKITGANFQALQVSLAYFKRDILADVFS RAMLTTGNHRHPFLYRIDVSHCSSLRDFYVAYLGERRKYFEDVAKKIAK NKLNTPCHILRRLQREGSGEEAGKDVKPKFLPRGIFTDSIKNCLEQSKL NIYIRNARNDVKPAINAAYLILMYYKEIEKGEFQGFYGEKRRYDILEEGK PLDLAERKKALASIKPAKIDVSEANMPMSKEEHLMRKRYHAVCNNES AIRMYQVQDILLLLMAKDIFKKALSEGVMSKKIGLENLNGIFDAPVNFV KNFDNIKLTATGIKIKDYGKVCRLGTDFKFNSLIKAFHKVYSKSVEMDYS DYLKEEEEFEKYRLNMVKLCREVERGITEDLHLSLDGKSHLSFNDDVIKP YNDKYNVFNGGDLTFFINARNMFMHGDYKYECVKYVVSEHFKGSLN DVSFAKETYGHFCNLLESMRKKTGLRIDI |
| 68 | cas12g | MLPTRYKPARTLVRPLGRLPHEPRKEFVEKCRRVRMHFEQFNIDVADL CQWLMSLRPNTRIGDAQSTVFWDFFLNPSILTVEADEKERDRWRLAA FDELLQIRFGHDPNAPPWSEEFRSAIRHVAQRPKSATAQRLFDRLRSL TAPHRLVLLKSAAEWIIARYQRGMENWQRQFAEWQREKEEWEAAH PNLTPEVRDAFTRVFKNLFENPDGDGKIGVRRKNPRICSWERLKLNKD NCVYAGQKGHGPLCWEFSKFVKAQKNAGTIKTFFVDVANKYLHVRR NLSKPGVKLKKSPRQEAFKRLYNQKGMEKARNWFTDAWSGYLTALN LNEKTILDHGCLKHCGAIGAEFEKSLCQFNPHTHLCVQYRNALESLEPA IRELEGDYREWRRLFLAPPRKPSFRYPSSRRLPMPKIFGEHFHQIDFDQ SILRLRLEDMAEGEWIEFGFKPWPKDYRPGKDEVRVTSVHVNFHGNR MRAGFHFEAPAKPSRFACTQDELDDLRSKQFPRQSQDRQLLEVARRR LLESFDGMLESDLRILAVDLGEKGAAAAVYQGHGHEADVAIPIVKIDRL YDHVPDVLDVESARVPPPKFDDSRDPRGVRKEHVGRHLGQLQRGAQ TLAQHRQQDESAPAALRRHDFRSLTRHIRWMIRDWTRHNAAQITAA AETHRCHLIVFESLRGFKPRGYDQMDFAQKARLAFFAYGRVRRKVVE KAVERGLRVVTVPYGFTSQICSECGHRQRNKGRLRKNKYQRRFVCEC GEPKKSANKTAAPDRSATVSPCTCRLQLGSDVNAARVLARVFWDEIV LPTREEMREPAVDSAPPSK |
| 69 | cas12g | MCLCTLSGRTRQEEEIIGSTQYTEARSLVRRIRRPRGESRRQFKSNVLLL RRHFEQFNVDASEICQWLMGIRPGGRHADESTGPFWEFFLDPGRFLR ETGRGPEDADERIDAYRRIAFDVVAGIEDESRMSDPSIPRQIVESLHAV SMATRTESARRLFERLAGLEPSHRQILLKAAAEWIVSRYWRSVQGWP DRYKHWSDEKEEWEKAHPRLTESLREEFTGIFRDLGIRRKKPRVCPWE RLEKGMDNCMYAGERIKVGYSRQSHSQLCAKYERFSYKQRQRTKSGK NFKSYFVKNAELYLKLRRKNRSLIKKDVMKLFRKKVPQALWFEKAWDE YLKALGVDEATLTKDGKLPHCTQFADDKECLFNRHTELCLQYRERLLRL PHLQELEQLYREWRDKYLSGPRRPSLRYPSKRTLPMPKVFGRGYFCAD FTNSLLDLRLEGMGEGDFVRFGFAPWPADYDAQPSDATVTSVHIHFV GTRARAGFRFQAPHKTSRFASSQDEIDDLRSRKFPRAAQDGEFLDAA RKLLLESFTGDAEREMKLLAVDLGDRGAGAAVFEGRCFKEAMPLKIIK TDTLIDKPPPVTKTPRKGKPGKRESKRARGLDKYHVARHLDTWRKGA RKIAERRAKGEADPVKLGAHDMRSLSLHVRWMIRDWVRLNASQIIKT AESHKTDLIVLESLRGFSAPGYHKLDDEKKRTLAFFAYGRIRRKLTEKAV ERGMRVVVAPYLRSSQVCAECGREQIDRNKLMKDKRKRRFICEYSDC TWQCDSDQNAACVLGRVFWGEIELPSERKKD |
| 70 | cas12g | MHPSRYKTARTLVRRLCRLPGEDRSAFRSKVGLLRGHFEQFNVDVSEL CQWLMSLRKRNKVPENPATFGALGDFLLQPGLPGEETDEKEADRLRL AVFDAVAGFRMLEDRLAASIPASLSDAIRDEAVFLAGVRAAGKPSGLA RVLARLEACAPAQRLVLLKSAAEWIVARFLRGTENWMRQRAEWEKE KAAWEAAHPHLTPEVRAQFNKIFESLHDPENSGKPGVSRKNPRICPW DRLKQNLDNCCYGEKGHSALCWRYQDFLKQRMGENRRDKKNFSAT AMDLAQICREWKIQHSRNALNNPRVLDRLFAEHERRKQDKTKKESRS PKPRQGGYKANPKADYLRSFKAHWKAYLEHMKLNDTTVLERGCLPH CLSIKKNGKESTCKWNKHTELCLEYKRSLAPLPDSVLELEPEYREWRRL YLHGPGRPHFRYPSAGELPLPKVFGEGFHQVDLDRSIVRLRLEGAAEG EWLEFGFIPWPRGYQPSRREVLITSVQVHFVGTRPRAGFRFDVSHRTS RFGCSQDELDELRSRRYPRQAQDKEFLAAARAQLIQTFEGGEGAARQ QMRVMSVDLGEGGACASIYEGRTHQKDESLKVIKIDRRYDQHPEVLE KDVGAAKPQKFEKSDPRGVRKEHVARHLNRIAAGASAIAEHRRKERS DAECSVGELQEHDFRSLKRHIAWMIRDWVRLNAAQIIDVAKQHCCDL IVFESQRGFRLPGYDELDRGKKQRFAILAFGRIRRKVVEKAVEHGMRV VTVPYFASSQVCSACKRVQENRGSWRENKKKRVFACEFCKLKLNSDA NASRVLARVFWGEIELPEPTRAHLPSKA |
| 71 | cas12g | MPVSRYSESRTLVRPLARLPHEERQDVTPKVARLRRHFERFNVDVAEL CQWLMGLRNQFGPKESPASFGPLGDFLIEPALDNIDADETERDRWRL AVFDAVAGFRPIRGLGDHPVPDTLRLAMQQAASLSPTPTTARLLERLR PLSPAHRLVLLKSAAEWIVARYQRGMENWVIQHAAWHKEKEAWER EHPALTPAVRERFTALYKQLSDSKPTDRPVSRRKNPRICEWERLRQNID NCCYAGEKGHGPLCRKYANFVKARKAVDGKFNDLLFWDTATSFIALC RKFNVTRARNALQSQLDALFAEDQRRKAERDQAKGRQPRPLHPQAA ARAKSDFLRIFKDGWNAYLSAMGLNDSTAIEKGRLPHCQKIGGTFENS KCEWNPHTDLCHQYRRLAGQLDDATLALEKDYREWRRLYLAGPRKPS FQYPSSRDLPMPKIFGAGFFELDMDRSILRLRLDDMVEGEWLEFGFKP WPREYTPSRAQVARPGRITSVHVNFIGSRCRVGFRFEAPHAGSRFGCS QDEIDQLRRDHPRERDDQPFLEAARKRLVETFAGDARRDLRLLAVDV GEKGCCAAVYQGTRYVADALLPIIKINQLYTEPPTELKPDSHNRPAPDR RPFNDEKDPRDPRGVRKEHVARHLKRMADKAPEVAAYRLAQREKAA PSPSASPPPVTLGVHDFRRLKRHVTWMIRDWARHNAARIVAEAQRH GCDLIVFESHRGRRPPGYHEVGDDAERRKLDNATFAFGRIRRKVTEKA VERGLRVVTVPYHCSSKVCSRCGRLQENDGLLRRNKKERKFICEQCKF ETNSDGNAARVLARVFWGEIMLPSPEERRKKREGSGGRSPTPANPG GLVDAPPSRRNLR |
| 530 | Cas12 | MEDYSGFVNIYSIQKTLRFELKPVGKTLEHIEKKGFLKKDKIRAEDYKAVKKIIDKYHRAYIEEVFDSVLHQKKKKDKTRFSTQFIKEIKEFSELYYKTEKNIPDKERLEALSEKLRKMLVGAFKGEFSEEVAEKYKNLFSKELIRNEIEKFCETDEERKQVSNFKSFTTYFTGFHSNRQNIYSDEKKSTAIGYRIIHQNLPKFLDNLKIIESIQRRFKDFPWSDLKKNLKKIDKNIKLTEYFSIDGFVNVLNQKGIDAYNTILGGKSEESGEKIQGLNEYINLYRQKNNIDRKNLPNVKILFKQILGDRETKSFIPEAFPDDQSVLNSITEFAKYLKLDKKKKSIIAELKKFLSSFNRYELDGIYLANDNSLASISTFLFDDWSFIKKSVSFKYDESVGDPKKKIKSPLKYEKEKEKWLKQKYYTISFLNDAIESYSKSQDEKRVKIRLEAYFAEFKSKDDAKKQFDLLERIEEAYAIVEPLLGAEYPRDRNLKADKKEVGKIKDFLDSIKSLQFFLKPLLSAEIFDEKDLGFYNQLEGYYEEIDSIGHLYNKVRNYLTGKIYSKEKFKLNFENSTLLKGWDENREVANLCVIFREDQKYYLGVMDKENNTILSDIPKVKPNELFYEKMVYKLIPTPHMQLPRIIFSSDNLSIYNPSKSILKIREAKSFKEGKNFKLKDCHKFIDFYKESISKNEDWSRFDFKFSKTSSYENISEFYREVERQGYNLDFKKVSKFYIDSLVEDGKLYLFQIYNKDFSIFSKGKPNLHTIYFRSLFSKENLKDVCLKLNGEAEMFFRKKSINYDEKKKREGHHPELFEKLKYPILKDKRYSEDKFQFHLPISLNFKSKERLNFNLKVNEFLKRNKDINIIGIDRGERNLLYLVMINQKGEILKQTLLDSMQSGKGRPEINYKEKLQEKEIERDKARKSWGTVENIKELKEGYLSIVIHQISKLMVENNAIVVLEDLNIGFKRGRQKVERQVYQKFEKMLIDKLNFLVFKENKPTEPGGVLKAYQLTDEFQSFEKLSKQTGFLFYVPSWNTSKIDPRTGFIDFLHPAYENIEKAKQWINKFDSIRFNSKMDWFEFTADTRKFSENLMLGKNRVWVICTTNVERYFTSKTANSSIQYNSIQITEKLKELFVDIPFSNGQDLKPEILRKNDAVFFKSLLFYIKTTLSLRQNNGKKGEEEKDFILSPVVDSKGRFFNSLEASDDEPKDADANGAYHIALKGLMNLLVLNETKEENLSRPKWKIKNKDWLEFVWERNR |
| 531 | Cas12 | MKKFTNLYSLSKTLRFELIPQGKTLENIQKSGILEQDNSRAEKYEKIKKIIDDYHKFFIEKSFTGKKIDDYFLNQYFELFKIKDKDEEQKKDFKSIQENLRKNIISFFDKNKLKRLFEKEIIKEDLPNFVKEEEDKKLISEFDKFTTYFVGFHENRKSMYSEEEKSTSIAYRTINENLPKFINNIFVFEKISKTPISENFRELYKDLEEYLNVNDIQDIFKLNYFSNVITQKQIDVYNLVIGGKTLENGTKIKGLNEYINLYNQNQTDKKNKLPLLTVLFKQILCDRDTISFLPEQFENDIDVLDNIKNTYSNMEKSIKDIKDLLSNLKDFDLSKIYITNDIALTDISQQVFNNYSIIINAIKENIKKENPKKKTENEEKYGERIDKIFKSNNSFSIKYINDCIKEKNIEIYFMDFGKKENNKKVKNLFDELQNNYSMVKDLLEYKKIQSLIQDEKSIELIKNFLDSIKNIQHFLKPLYVKDNDIVKDISFYRDFEELYLNIDKITPLYNKVRNYVTQKPYSVKKIKLNFENSTLLAGWDLNKERDNTCAILRKDDLYYLAIMDVNNRNVFNEKGIDGIGYEKMEYKLLPGANKMLPKVFFSKSRIKDFNPSEQIIRNYEKETHKKGSNFSLKDCHKLIDFFKSSINKHEDWKNFNFKFSNTDKYEDLSGFYREVEQQGYKITFRNISKEYVDKLVEEGKIYLFQIYNKDFSKYSKGTPNMHTLYWKALFDEDNLKNVVYKLNGQAEIFYRKGSIEKENIVIHKANNAIENKNMDNKKKQSKFEYDIIKDRRYTVDKFQFHVPITLNFKAIGNERINEQVNQYIKDNNIKHIIGIDRGERHLLFLSLIDLKGNIIKQFSLNEIVNEYNGNSYKTNYHMLLEKREEERDKARKSWKTIENIKELKEGYISQVIHKITQLMIEYNAIVVLEDLNFGFMRGRQKVEKQVYQKFEKMLIDKLNYLVDKKKDKNEAGGLLKAHQLTNKFESFQKMGKQNGFLFYIPAWNTSKLDPITGFVNLFDTHYTNVDNAKKFFENFEDIRFNEKKNYFEFIVNDYTKFNTKAEGTKLNWTICSNEDRIKTFRSSSKNNQWVSETVNLTDSLIELFKKYDIDYKLELKEQIISKSEKNFFETLLYLFKLTLQMRNSITGTETDYLISPVADKTGNFFDSRKGIENLPNNADANGAYNIARKGLWVIEQIKKAKDLKKVKLAISNKEWLQFVQGK |
| 532 | Cas12 | MAKNTIFSQFTGLYPVSKTLRFELKPMGKTLEKIKETGVIENDKKRHNDYFDAKKIIDKYHKYFIDAALSKFPRIDWSPLKEAIERSLDRSDASKKKLEKTQTEFRKKIAKALTTHDHYKELTASTPKDLFLKVFPDHFGKQPAIDTFDGFSSYFTGFQENRQNIYSDEAISTAIPYRLVHDNFPKFLSNIEVYKTLKDNAPSVLSDAENELRDFLNGKSLANIFELNAYNEVLTQSGIDFFNQVIGGISDEGGEKKTRGINEFSNLYRQQHPEFAQKRLATKMIPLYKQILSDRETKSFILESYSNDSQVQNSVKEFFESQILNWDIAGRRVNVLNELTSLVKRISEFDLGNIYVNQEELSNISLKLFDNWNSINGLLFKHAENRIGSAEKSANKKKIDAWMKNKEFSIATLNLAIAESNSEEISRVKIESYWNNFEAKVQSILCGDNRRNLDEFISATFNENNALREDSKIIEKLKAFLDALIEIMHSIKPLISDAENRDLSFYNELIPLYDQLSLVVPLYNKIRNYATQKLTESEKFKLNFDNPTLADGWDQNKEEANTAILLLKNGLYYLGIMNAKNKPKIKDFKTSESEDCYDKMVYKLLPGPNKMLPKVFFSEKGLATFKPPKDILDGYNAGKHKKGDLFDIGFCHQLIDFFKESIAKHPDWKKFDFNFSDTSSYEDISGFYKEVTDQGYKITFSKIPTSQIDEWVKEGKLFLFQIYNKDFAPGAKGSPNLHTLYWKSVFSPENLKDVVVKLNGEAELFYRPSSVKKPYSHKVGEKLVNRIGKDGLPLPESVFGELFRYFNGKLEGELSDEAKRYLDVAVVKDVKHEIVKDRRYTQDKFEFHVPLTLNFKADSKNEYMNERVRHFLKDNPDVNIIGIDRGERHLLYMTLINQKGEILKQKSFNVVESVNYQAKLVQREKERDAARRSWSSVGKIKDLKEGFLSQVIHEITTTMIENNAIVVLEDLNFGFKRGRFCVERQVYQKFEKMLIDKLNYLVFKNKPEGDVGGVLKGYQLAEKFDSFQKLGKQSGFLFYIPAAYTSKIDPTTGFANLFNMTELTSAEKKKEFLSHFEDITYDGKNDRFLFSFDYKNFKCFQTDYIKKWTVYSQGKRIVYDKESKSAKEISPVEIIKAALAKQNIALTDQLDVLSAINSVEASPKSASFFGDICYAFEKTLQMRNSIPNTDEDYLASPVMNKRGEFYDSRSCDDALPQNADANGAYHIALKGLYLIKNVFDAGGKELKISHEDWFKFAQSRNC |
| 533 | Cas12 | MSKGKIWENFINQYSVSKTLRFELKPVGKTLENINAKGLIEEDEQRAEDYKKAKKIIDEYHKYFIEGALGSCSLDLNILNEFLQLYNKAQKTDADKKEYEKIQTTLRKNIAESFGKNADKKTKEQYENLFKKELLRNDLPDWVEDEEDAKIIERFKTFTTYFTGFHENRKNIYDNEEKSTAIGYRIVHENLPKFIDNMNAFEKISKALDLSEIDRDFQSELGEIKAEEFFTIEFFNQCLNQFGIDRYNTLLGGISEGENIKKKQGLNERINLYNQQLKGERKKERLPKLKVLYKQILSDSSSHSFSIDEFENDNELLESLEIFYKNELIGFNHSGVDSNIFDLVKDLLLKIDESEQSSIYLKNDKGLTEISQRIFGDWNIIKSALEEYYDEHYPPKKDTFNKKELDERSRWLKENHSIGVIEKALANYENEIVREHLKQNSAPIVSYFKSLEVDGENLIDKIYSAYGNISDLLNSSYPDEKKLVSDRTSKDKIKVFLDSLMSLLHFLKPLDVKDLGNKDSAFYGDYDFIVEQLSKLVRLYNKTRNYLTRKPYSIEKIKLNFENSTLLAGWDVNKERDNNCVIFKRQDGDRELFYLGIMDKSHNKIFTKIEEAKSDDVYQKMNYKLLPGPNKMLPKVFFSKKSIDFYAPGEELLKNYKNGTHKKGENFNLQHCHELIDFFKRSINKHEDWSQFNFKFSDTSEYEDTSFFFKEVSQQGYSITFKNIDRETIEKFVDEGKLYLFQIYNKDFSPKSKGRPNLHTLYWKMLFDERNLANTVYQLNGEAEVFYRKKSISEKDRVVHRADEPIGLKNSENSAQKSLFPYDIVKDRRFTVDKFQFHVPITLNFKSEGNERLNISVNKFLKDNPDVNIIGLDRGERHLIYLTLINQKGEILHQESLNEVMGVNYQQKLHRVEKDRTEERRNWDRIENIKELKSGYLSQVVHKISQLMVEYNAIVVMEDLNFGFKRGRIKVEKQVYQKFEKTLIDKLNYLVFKDREPEEPAGVLNALQLTNKFESFKKLGKQCGFLFYVTSDYTSKIDPATGFVNLLYPKYESVEKSQNFFRKFDNICFNSGAGYFEFDFDYSNFTDRADGTRTRWKVCTVGNERFGYNPKTKASETVNVTESLKELLLQHEIAFENGESLVESISKNTTKYFHKSLLNFLRLTLTLRHSKTGTDIDYILSPVANEEGVFFDSRNASDKMPKDADANGAYNVALKGLMVLERINAAEDLSQFKFKDMSIKNKDWLKFVQDRQG |
| 534 | Cas12 | MKNLANFTNLYSLQKTLRFELKPIGKTLDWIIKKDLLKQDEILAEDYKIVKKIIDRYHKDFIDLAFESAYLQKKSSDSFTAIMEASIQSYSELYFIKEKSDRDKKAMEEISGIMRKEIVECFTGKYSEVVKKKFGNLFKKELIKEDLLNFCEPDELPIIQKFADFTTYFTGFHENRENMYSNEEKATAIANRLIRENLPRYLDNLRIIRSIQGRYKDFGWKDLESNLKRIDKNLQYSDFLTENGFVYTFSQKGIDRYNLILGGQSVESGEKIQGLNELINLYRQKNQLDRRQLPNLKELYKQILSDRTRHSFVPEKFSSDKALLRSLLDFHKEVIQNKNLFEEKQVSLLQAIRETLTDLKSFDLDRIYLTNDTSLTQISNFVFGDWSKVKTILAIYFDENIANPKDRQRQSNSYLKAKENWLKKNYYSIHELNEAISVYGKHSDEELPNTKIEDYFSGLQTKDETKKPIDVLDAIVSKYADLESLLTKEYPEDKNLKSDKGSIEKIKNYLDSIKLLQNFLKPLKPKKVQDEKDLGFYNDLELYLESLESANSLYNKVRNYLTGKEYSDEKIKLNFKNSTLLDGWDENKETSNLSVIFRDTNNYYLGILDKQNNRIFESIPEIQSGEETIQKMVYKLLPGANNMLPKVFFSEKGLLKFNPSDEITSLYSEGRFKKGDKFSINSLHTLIDFYKKSLAVHEDWSVFNFKFDETSHYEDISQFYRQVESQGYKITFKPISKKYIDTLVEDGKLYLFQIYNKDFSQNKKGGGKPNLHTIYFKSLFEKENLKDVIVKLNGQAEVFFRKKSIHYDENITRYGHHSELLKGRFSYPILKDKRFTEDKFQFHFPITLNFKSGEIKQFNARVNSYLKHNKDVKIIGIDRGERHLLYLSLIDQDGKILRQESLNLIKNDQNFKAINYQEKLHKKEIERDQARKSWGSIENIKELKEGYLSQVVHTISKLMVEHNAIVVLEDLNFGFKRGRQKVERQVYQKFEKMLIEKLNFLVFKDKEMDEPGGILKAYQLTDNFVSFEKMGKQTGFVFYVPAWNTSKIDPKTGFVNFLHLNYENVNQAKELIGKFDQIRYNQDRDWFEFQVTTDQFFTKENAPDTRTWIICSTPTKRFYSKRTVNGSVSTIEIDVNQKLKELFNDCNYQDGEDLVDRILEKDSKDFFSKLIAYLRILTSLRQNNGEQGFEERDFILSPVVGSDGKFFNSLDASSQEPKDADANGAYHIALKGLMNLHVINETDDESLGKPSWKISNKDWLNFVWQRPSLKA |
| 535 | Cas12 | MNLIENETKSEEIKSKLDSIMEIMHWTKMFIIEEEIEKDVNFYNEIEEIYDELQPLVTIYNRIRNYVTQKPYSEEKIKLNFGIPTLANGWSKTKEYDNNAIIMIRDGKYYLGIFNAKNKPDKKIMEGHQSEENGDYKKMIYRLLPGPNKMLPKVFMSKTGIAEYKPSQYILECYEQNKHIKSDKNFDIKFCRDLIDFFKTSINRHPEWSKFNFKFSETSEYEDISTFYREVEKQGYKIEWTYISEKEIKELDENGQLYLFQIYNKDFSEKSKGKENLHTMYLKNLFSEENLKNIVLKLNGEAEVFFRKSSIKKPIIHKKGSVLVNKTYNENGERKSIPEEQYTEIYKYLNSIGTNELSEKSKKLMEEGKVEYYKANYDIVKDYRYSVDKFFIHLPMTINFKAAGFSPINNIALKSIALKEDMHIIGIDRGERNLIYVSVIDTKGNIVEQRNFNIVNGIDYKEKLKQKELDRDNARKNWKEIGKIKDLKEGYLSLVVHEIAKLVVKYNAIITMEDLNQGFKRGRFKVERQVYQKFETMLINKLNYLVDKDLAVDQEGGLLRGYQLTYIPESLKVLGRQCGYIFYVPVAYTSKIDPTTGFVAIFNYKGMTDKDFVTSFDSIKYDDERGLFAFEFDYENFVTHKVEMARNKWTVYTYGERIKRKFKNGLWDTAEKVDLTYQMRSILEKYEIEYNKGQDILEQIEELDEKAQNGICKEIKYLVKDIVQMRNSLPDNAVEDYDAIISPVINNNGEFFDSTRGDEDKPLDADANGAYCIALKGLYEVMQIKKNWNEETEFPRKELKIRHQDWFDFIQNKRYL |
| 536 | Cas12 | MENRYQVLQGLTAAQKKAAAAAKKRSSFSIVELNAATRSRVPDEKYVPVQNYFSAMGKVCSQGEPKRENFVTRICAAYQELEEYIPSIRKSLLQEKRATELIKNYLDAVNDLLRFIKPLLGRGNETDKDANFYGEFSFLTDCLFAIVPLYNEVRNYLTQKPYSTEKFKLNFRGSTLLNGWDKNKERDNLGVILRKEGKYFLAIMNKKHNTLFTEGKLQQHTGGECYQKMEYKLIPGSKMLPKVFFSKKGISTFQPSEELLLNYRIGTYKKGEKFNLEHLHKLIDFYKHSIAVHEDWSKFDFHFSDTSSYRDISGFYKEVEQQGYKLTFRNVSVSYINRLVEEGKLYLFQIYNKDFSEYSKGTPNLHTLYWKMLFDPENLKDVVYKLSGEAEVFFRKKSLDVSHPTHPKNEPIEKKNINNKGEKSLFSYDLIKDRRFTVDKFQFHVPITMNFKGEQGDRVNQMVQSYVRNNKGLNVIGIDRGERNLLYLVVINEHGEILEQFSLNEIRNAYNGKEHKIDYHTLLEERSKKRQDARQSWQTIEGIKDLKTGYLSQVIHVITQLMVKYNAIVVLEDLNFGFKSSRQKFEQSVYQQFERKLIDKLNFLVNKKAAPNEVGGLLNAYQLTAPLGNSRKMGKQNGFLFYVPAWHTSKIDPRTGFVNLLDTRYENVAKAKEFFAKFASITYNPEKKWFEFAFDYKAFGNRADGSRTKWTICSYGERIETFRNPENNNQWDTKSVPLTERLTELFSKYGIDYTTNLKEQILNQTDKAFFVELLGALRLTLQLRNSRKSTGEDFLFSPVADENGCFFDSREANDNEPKDADANGAYHIALKGLWVLDTIRNTEEGKNPKLAITNKEWLSFAQAKPFAHE |
| 537 | Cas12 | MSDSYDELTKAQKEKQEKRKHVALTEVVAALEKYTIALDNGHEHKNAVNTFKNYFQNYFFHFDTDKKKTAKTLDCQIKDEYNGLKGILNTPWDKNKKLQQDKKLVQQIKSFLDSIQELLWFIKPLVLTDNTLEKDERFYGEFMPLYDEISNIIKLYNKIRNYLTKKPYSIEKYKLNFENGSLLSGWDVNKEKDNTSVLLCKDNQYYLAIMHIDHNKVFELDELIKHAGKGYQKINYKLLPGANKMLPKVFFSGKNISYYDPSKEILKIRNYGTHTKNGDPQPGFSKRDFSVDDCRKMIDFFKNSIAKHEDWKNFDFKFQPTKNYNSIDEFYREVEEQGYKITYSNVSEDYIDSLVEYGKIYLFHIYNKDFSDKRDESKKHTDNMHTLYWKALFDAKNLKDVVYKLNGEAEIFYRKKSIDIKKPTHEKGKPIDNKNPNARKKTSVFKYDLIKDKRFTVDKFFFHVPITLNFKSKSGYLSNDDVNAAIKKNNDIKIIGLDRGERNLIYLSLINSKGEIAYQESLNVVSTDKGFDVNYHKLLDDKEGNRDEARKNWDKIENIKELKAGYLSQVIHKIAKLMIDNNAIVVMEDLNFGFKRGRFKVEKQIYQKFEKMLIDKLNYLVFKNVHPEQAGGLYKAYQLTAQFESFKKLGKQSGFLFYIPAWNTSKIDPTAGFVDFLKPRYESVTQAKSFLQRFDKINYNKTKDYFEFAFDYKNFTDKANDTKTDWVVCTYGTERYYYDVRTKTTQKIDITAELKKLLEKSEINYLNGKDIKELIIAVDSKEFHSALLKYLAIVLALRYSDSQSGRDFILSPVANEQGHFFNSDKTDDTLPKDADANGAYHIALKGLWAINQIRKTKNGDKLKLTISNKDWLNFVQKKEYRKGV |
| 538 | Cas12 | MQTLFENFTNQYPVSKTLRFELIPQGKTKDFIEQKGLLKKDEDRAEKYKKVKNIIDEYHKDFIEKSLNGLKLDGLEKYKTLYLKQEKDDKDKKAFDKEKENLRKQIANAFRNNEKFKTLFAKELIKNDLMSFACEEDKKNVKEFEAFTTYFTGFHQNRANMYVADEKRTAIASRLIHENLPKFIDNIKIFEKMKKEAPELLSPFNQTLKDMKDVIKGTTLEEIFSLDYFNKTLTQSGIDIYNSVIGGRTPEEGKTKIKGLNEYINTDFNQKQTDKKKRQPKFKQLYKQILSDRQSLSFIAEAFKNDAEILEAIEKFYVNELLHFSNEGKSTNVLDAIKNAVSNLESFNLTKMYFRSGTSLTDVSRKVFGEWSIINRALDNYYATTYPIKPREKSEKYEERKEKWLKQDFNVRLIQTAIDEYDNETVKGKNSGKVIADYFAKFCDDKETDLIQKVNEGYIAVKDLLNTPYPENEKIGSNKDQVKQIKAFMDSIMDIMHFVRPLSLKDTDKEKDETFYSLFTPLYDHLTQTIALYNKVRNYLTQKPYSTEKIKLNFENSTLLGGWDLNKETDNTAIILRKDNLYYLGIMDKRHNRIFRNVPKADKKDFCYEKMVYKLLPGANKMLPKVFFSQSRIQEFTPSAKLLENYANETHKKGDNFNLNHCHKLIDFFKDSINKHEDWKNFDFRFSATSTYADLSGFYHEVEHQGYKISFQSIADSFIDDLVNEGKLYLFQIYNKDFSPFSKGKPNLHTLYWKMLFDENNLKDVVYKLNGEAEVFYRKKSIAEKNTTIHKANESIINKNPDNPKATSTFNYDIVKDKRYTIDKFQFHIPITMNFKAEGIFNMNQRVNQFLKANPDINIIGIDRGERHLLYYALINQKGKILKQDTLNVIANEKQKVDYHNLLDKKEGDRATARQEWGVIETIKELKEGYLSQVIHKLTDLMIENNAIIVMEDLNFGFKRGRQKVEKQVYQKFEKMLIDKLNYLVDKNKKANELGGLLNAFQLANKFESFQKMGKQNGFIFYVPAWNTSKTDPATGFIDFLKPRYENLNQAKDFFEKFDSIRLNSKADYFEFAFNFKNFTEKADGGRTKWTVCTTNEDRYAWNRALNNNRGSQEKYDITAELKSLFDGKVDYKSGKDLKQQIASQESADFFKALMKNLSITLSLRHNNGEKGDNEQDYILSPVADSKGRFFDSRKADDDMPKNADANGAYHIALKGLWCLEQISKTDDLKKVKLAISNKEWLEFVQTLKG |
| 539 | Cas12 | MQLTDNLSDKYKEAAPLLNENYSNEKGLKNDDKSISLIKNFLDAIKEIEKFIKPLSETNITGEKNDLFYSQFTPLLDNISRIDILYDKVRNYVTQKPFSTDKIKLNFGNSQLLNGWDRNKEKDCGAVWLCKDEKYYLAIIDKSNNSILENIDFQDCDESDCYEKIIYKLLPGPNKMLPKVFFSEKCKKLLSPSDEILKIRKNGTFKKGDKFSLDDCHKLIDFYKESFKKYPNWLIYNFKFKKTNEYNDIREFYNDVASQGYNISKMKIPTSFIDKLVDEGKIYLFQLYNKDFSPHSKGTPNLHTLYFKMLFDERNLEDVVYKLNGEAEMFYRPASIKYDKPTHPKNTPIKNKNTLNDKKTSAFPYDLIKDKRYTKWQFSLHFPITMNFKAPDRAMINDDVRNLLKSCNNNFIIGIDRGERNLLYVSVIDSNGTIIYQHSLNIIGNKFKGKTYKTNYREKLATREKDRTEQRRNWKAIESIKELKEGYISQAVHVICQLVVKYDAIIVMEKLTEGFKRGRTKFEKQVYQKFEKMLIDKLNYYVDKKLDPDEEGGLLHAYQLTNKLESFDKLGTQSGFIFYVRPDFTSKIDPVTGFVNLLYPRYENIDKAKDMISRFDEIRYNAGEDFFEFDIDYDKFPKTASDYRKKWTICTNGERIEAFRNPANNNEWSYRTIILAEKFKELFDNNSINYRDSDDLKAEILSQTKGKFFEDFFKLLRLTLQMRNSNPETGEDRILSPVKDKNGNFYDSSKYDEKSKLPCDADANGAYNIARKGLWIVEQFKKADNVSTVEPVIHNDKWLKFVQENDMTNN |
| 540 | Cas12 | MLPNEKERNEFKNSNAKQYIREISNIITDTETAHLEYDEHISLIESEEKADEMKKRLDMYMNMYHWAKAFIVDEVLDRDEMFYSDIDDIYNILENIVPLYNRVRNYVTQKPYNSKKIKLNFQSPTLANGWSQSKEFDNNAIILIRDNKYYLAIFNAKNKPDKKIIQGNSDKKNDNDYKKMVYNLLPGANKMLPKVFLSKKGIETFKPSDYIISGYNAHKHIKTSENFDISFCRDLIDYFKNSIEKHAEWRKYEFKFSATDSYNDISEFYREVEMQGYRIDWTYISEADINKLDEEGKIYLFQIYNKDFAENSTGKENLHTMYFKNIFSEENLKDIIIKLNGQAELFYRRASVKNPVKHKKDSVLVNKTYKNQLDNGDVVRIPIPDDIYNEIYKMYNGYIKENDLSEAAKEYLDKVEVRTAQKDIVKDYRYTVDKYFIHTPITINYKVTARNNVNDMAVKYIAQNDDIHVIGIDRGERNLIYISVIDSHGNIVKQKSYNILNNYDYKKKLVEKEKTREYARKNWKSIGNIKELKEGYISGVVHEIAMLMVEYNAIIAMEDLNYGFKRGRFKVERQVYQKFESMLINKLNYFASKGKSVDEPGGLLKGYQLTYVPDNIKNLGKQCGVIFYVPAAFTSKIDPSTGFISAFNFKSISTNASRKQFFMQFDEIRYCAEKDMFSFGFDYNNFDTYNITMSKTQWTVYTNGERLQSEFNNARRTGKTKSINLTETIKLLLEDNEINYADGHDVRIDMEKMDEDKNSEFFAQLLSLYKLTVQMRNSYTEAEEQEKGISYDKIISPVINDEGEFFDSDNYKESDDKECKMPKDADANGAYCIALKGLYEVLKIKSEWTEDGFDRNCLKLPHAEWLDFIQNKRYE |
| 541 | Cas12 | MSNLYSNLHNLYPVQKTLRFELKPQGKTKENMEKAGILKADEHRAEVYGKVKKYCDEYHKTFIDRCLSNIELNEIDKYYELYSINNRDDKQKEELDQLETGLRKQISDAFKKSAEYKGLFQKDMITSYLVTMYKENQEKMQDIGEFNRFTTYFTGYNKNRENMYSEEDKSTAISYRLINENLPTFIDNIKIYKKIVSLMPENIEKIYKDLEEYIQVNSVDEIFNISYYNDVLTQRGIECYNILISGRTKNDGDKIKGLNEYINEFNQTHNEKIPKLQELYKQILSDAESASFKVDIIENDKELLNLIEVYYANILPTLNKIEDLFTRISNYNLELILVNNDGSLSTLSNMVFNEWSYIKGIISQKYDAEYSGKEKYGTEKYAQKKQEYLKKQKIYSLKFLNDCIGNNAICEYLKNYIIQNKNIETIKEDYNEVQNIKAEDDTKELIKDEKSIEKIKKFLDDVKSLQEFVKLVIPKDRTVEKDAKFYSELTPYYEKIKEIIPLYNKVRNYVTQKPYSTEKIKLNFECPTLLNGWDANKEEANLGVILLKEGKYYLGIMNPYCKKIFEVYEKDSNEQNNYKKMEYKLLPGPNKMLPKVFFSNSRIEEFNPSKELQEKYNKGYHKKGKDFDINFCHELIDFYKQSLNKHEDWKKFNFKFKDTSEYNDISEFYREVEEQGYKIEYTEYSEKYINELVDRGELYLFQIYNKDFSEYSKGKENLHTLYWKAVFDPDNIMNPVYKLNGNAEIFYRKKSLEMKVTHPANQPIANKNISTIEAGRSTSTFKYDLIKDKRYTMDKFQFHVPITVNFKSERLFNINQIVNKYLKYNDDIHVIGIDRGERNLLYVCVIDKNEKIVYQKSLNEIVSEYNNNRYTTDYHGLLDRKEKEREIAREDWKNIENIKELKEGYMSQIIHILVELMKKYNAIIVIEDLNKGFKNSRIKVEKQVYQKFEKMFIDKLNYLVFKDEDKMDEGGVLNAYQLTNKFESFTKLGKQSGILYYIPAWCTSKIDPTTGFINRFYLKYENFDKSKEFVNRIDDIRYNEKENLFEFDIDYSKFTDRLNDTKNKWTLCSYGERILTQKNANGEWFDRRIQLSIEFKNLFEKYVINLNNIKDSILKLDKDNIEFYKGNGENLGFIQLFKLMVQMRNSLTGKEEDNLISPVKNQHGKFFNTSERVEGLPIDADANGAYNIARKGFMLVEQMKNVEDEKLNKIKYNITEKEWLNYVQNRGMWWKRQYLYHI |
| 542 | Cas12 | MAKNTIFSQFTGLYPVSKTLRFELKPMGKTLEKIKETGVIENDKKRHNDYFDAKKIIDKYHKYFIDAALSKFPRIDWSPLKEAIERSLDRSDASKKKLEKTQTEFRKKIAKALTTHDHYKELTASTPKDLFLKVFPDHFGKQPAIDTFDGFSSYFTGFQENRQNIYSDEAISTAIPYRLVHDNFPKFLSNIEVYKTLKDNAPSVLSDAENELRDFLNGKSLANIFELNAYNEVLTQSGIDFFNQVIGGISDEGGEKKTRGINEFSNLYRQQHPEFAQKRLATKMIPLYKQILSDRETKSFILESYSNDSQVQNSVKEFFESQILNWDIAGRRVNVLNELTSLVKRISEFDLGNIYVNQEELSNISLKLFDNWNSINGLLFKHAENRIGSAEKSANKKKIDAWMKNKEFSIATLNLAIAESNSEEISRVKIESYWNNFEAKVQSILCGDNRRNLDEFISATFNENNALREDSKIIEKLKAFLDALIEIMHSIKPLISDAENRDLSFYNELIPLYDQLSLVVPLYNKIRNYATQKLTESEKFKLNFDNPTLADGWDQNKEEANTAILLLKNGLYYLGIMNAKNKPKIKDFKTSESEDCYDKMVYKLLPGPNKMLPKVFFSEKGLATFKPPKDILDGYNAGKHKKGDLFDIGFCHQLIDFFKESIAKHPDWKKFDFNFSDTSSYEDISGFYKEVTDQGYKITFSKIPTSQIDEWVKEGKLFLFQIYNKDFAPGAKGSPNLHTLYWKSVFSPENLKDVVVKLNGEAELFYRPSSVKKPYSHKVGEKLVNRIGKDGLPLPESVFGELFRYFNGKLEGELSDEAKRYLDVAVVKDVKHEIVKDRRYTQDKFEFHVPLTLNFKADSKNEYMNERVRHFLKDNPDVNIIGIDRGERHLLYMTLINQKGEILKQKSFNVVESVNYQAKLVQREKERDAARRSWSSVGKIKDLKEGFLSQVIHEITTTMIENNAIVVLEDLNFGFKRGRFCVERQVYQKFEKMLIDKLNYLVFKNKPEGDVGGVLKGYQLAEKFDSFQKLGKQSGFLFYIPAAYTSKIDPTTGFANLFNMTELTSAEKKKEFLSHFEDITYDGKNDRFLFSFDYKNFKCFQTDYIKKWTVYSQGKRIVYDKESKSAKEISPVEIIKAALAKQNIALTDQLDVLSAINSVEASPKSASFFGDICYAFEKTLQMRNSIPNTDEDYLASPVMNKRGEFYDSRSCDDALPQNADANGAYHIALKGLYLIKNVFDAGGKELKISHEDWFKFAQSRNC |
| 543 | Cas12 | MYKDKTDKTKIIDSDLIKFINIAESTQLDSMSQDEAKELVKEFWGFTTYFVGFYDNRKNMYTAEEKSTGIAYRLVNENLPKFIDNMEAFKKAIARPEIQANMEELYSDFSEYLNVESVQEMFQLDYYNMLLTQKQIDVYNAIIGGKTDDEHDVKIKGINEYINLYNQQHKDDKLPKLKALFKQILSDRNAISWLPEEFNGDQEVLNAIKDCYERLSENVLGDKVLKSLLGSLSDYSLDGIFIRNDLQLTDISQKMFGNWCVIQNAIMQNIKHVAPARKHKESEEDYEKRIAGIFKKVDSFSISFINDCLNEADPNNAYFVENYFATFGAVNTPTMQRENLFALVQNAYTEVAALLHSDYPTAKHLAQDKVNVAKIKALLDAIKSLQHFVKPLLGKGDESDKDERFYGELASLWAELDTVTPLYNMIRNYMTRKPYSQKKIKLNFENPQLLGGWDANKEKDYATIILRRDGLYYLAIMNKESKKLLGKAMPSDGECYEKMVYKLLPGANKMLPKVFFAKSRMEDFKPSKELVEKYNNGTHKKGKNFNIQDCHNLIDYFKQSISKHEDWGKFGFNFSDTSTYEDLSGFYREVEQQGYKLSFARVSVSYISQLVEEGKMYLFQIYNKDFSEYSKGTPNMHTLYWKALFDERNLADVVYKLNGQAEMFYRKKSIENTHPTHPANHPILNKNKDNKKKESLFDYDLIKDRRYTVDKFMFHVPITMNFKSSGSENINQDVKAYLRHADDMHIIGIDRGERHLLYLVVIDLQGNIKEQYSLNEIVNEYNGNTYHTNYHDLLDVCEEERLKARQSWQTIENIKELKEGYLSQVIHKITQLMVKYHAIVVLEDLNMGFMRGRQKVEKQVYQKFEKMLIDKLNYLVDKKADASVSGGLLNAYQLTSKFDSFQKLGKQSGFLFYIPAWNTSKIDPVTGFVNLLDTRYQNVEKAKVFFSKFDAIRYNKDKDWFEFNLDYDKFGKKAEGTRTKWALCTRGMRIDTFRNKEKNSQWDNQEVDLTAEMKSLLEHYYIDIHGNLKDAISAQTDKAFFTGLLHILKLTLQMRNSITGTETDYLVSPVADENGIFYDSRSCGDELPENADANGAYNIARKGLMMIEQIKDAKDLDNLKFDISNKSWLNFAQQKPYKNE |
| 544 | Cas12 | MNEADPNNAYFVENYFATFGAVNTPTMQRENLFALVLNAYTEVASLLHSYYPAEKNLAQDKANVAKIKALLDAIKSLQHFVKPLLGKGDESDKDERFYGELASLWAELETVTPLYNMIRNYMTRKPYSQKKIKLNFENPQLLGGWDANKEKDYATIILRRNGLYYLAIMDKDSRKLLGKAMPSDGECYEKMVYKLLPGANKMLPKVFFAKSRMEDFKPSKELVEKYNNGTHKKGKNFNIQDCHNLIDYFKQSISKHEDWGKFGFNFSDTSTYEDLSGFYREVEQQGYKLSFARVSVSYISQLVEEGKMYLFQIYNKDFSEYSKGTPNMHTLYWKALFDERNLADVVYKLNGQAEMFYRKKSIENTHPTHPATHPILNKNKDNKKKESLFEYDLIKDRRYTVDKFMFHVPITMNFKSVGSENINQGVKEYLHHADDMHIIGIDRGERHLLYLVVIDLQGNIKEQYSLNEIVNEYNGNTYHTNYHDLLDAREDERLKARQSWQTIENIKELKEGYLSQVIHKITQLMVKYHAIVVLEDLNMGFMRGRQKVEKQVYQKFEKMLIDKLNYLVDKKADASVSGGLLNAYQLTSKFDSFQKLGKQSGFLFYIPAWNTSKIDPVTGFVNLLDARYQNVEKAKAFFSKFDAIRYNKDKDWFEFNLDYDKFGKKAEGTRTKWTLCTRGMRIDTFRNKEKNSQWDNQEVDLTAELKSLLEHYYIDIHGNLKEAISTQTDKAFFTGLLHILKLTLQMRNSITGTETDYLVSPVADENGIFYDSRTCGDELPENADANGAYNIARKGLMMIEQIKNAEDLGNLKFDISNKAWLNFAQQKPYKNG |
| 545 | Cas12 | MDNNNLHAVDGYFATLGAVNTPTMQRENLFALIQNAYTDISDLLDTPYPENKNLAQDKTNVAKVKALLDAIKSLQHFVKPLLGMGDESDKDERFYGELASLWTELDTVTPLYNMIRNYMTRKPYSEKKIKLNFENPQLLGGWDANKEKDYATIILRRNGMYYLAIMDKDSKKLLGKTMPSDGECYEKMVYKLLPGANKMLPKVFFAKSRINDFKPSKKIVENYNNGTHKKGKNFNINDCHDLIDYFKQSIDKHEDWSKFGFNFSDTSTYEDLSGFYREVEQQGYKLSFTNISVSFIDKLVDEGKMYLFQIYNKDFSDYSKGTPNMHTLYWKALFDERNLADVVYKLNGEAEMFYRKKSINNTHPTHHANHPIQNKNKDNKKKESVFEYDLVKDRRYTEDKFLFHVPITMNFNSVGAENINQQVRKYLQQADDTHIIGIDRGERHLLYLVVIDMQGNIKEQFSLNEIVNEYNGNTYRTNYHDLLDVRADKRLKASQSWQTIENIKELKEGYLSQAIHKITQLMVKYHAVVVLEDLNKGFMRGRQKVEKQVYQKFEKMLIDKLNYLVDKHKDANETGGLLHALQLTSEFKNFKKSEYQNGFLFYIPAWNTSKIDPVTGFVNRFDTRYTNAVEAQKFFRKFDEIRYNEEKDWFEFEFDYDKFTQKAHGTRTRWTLCTHGKRLRSFRNPAKQYNWDSEVVALTDEFKRILGEAGIDIHENLKDAISNLEGKRRKYLEPLMQFMKLLLQLRNSRKNPEEDYILSPVADENGVFYDSRSCGDKLPENADANGAYNIARKGLMLIRQIKEAKELGKVKYDISNKAWLNFAQQKPYKNE |
| 546 | Cas12 | MQKKAFEENQQNLRSIIAKKLTEDKAYANLFGKNLLESYKDKTDKTKIIDSDLIKFINTAESTQLDSMSQDEAKEIVKEFWGFTTYFVGFFDNRKNMYTAEEKSTGIAYRLINENLPKFIDNMEAFKKAIARPEIQADMEELYSNFSEYLNVESIQEMFLLDYYNMLLTQKQIDVYNAIIGGKTDDEHDVKIKGINEYINLYNQQHKDDKLPKLKALFKQILSDRNAISWLPEEFNSDQEVLNAIKDCYERLSENVLGDKVLKSMLGSLADYSLDGIFIRNDLQLTDISQKMFGNWSVIQNAIMQNIKHVAPARKHKESEEEYENRIAGIFKKADSFSISYIDACLNETDPNNAYFVENYFATLGAVDTPTMQRENLFALVQNAYTEITALLHSDYPTEKNLAQDKANVAKIKALLDAIKSLQHFVKPLLGKGDESDKDERFYGELASLWAELDTMTPLYNMIRNYMTRKPYSQKKIKLNFENPQLLGGWDANKEKDYATIILRRNGLYYLAIMNKDSKKLLGKAMPSDGECYEKMVYKLLPGANKMLPKVFFAKSRMEDFKPSKELVEKYNNGTHKKGKNFNIQDCHNLIDYFKQSIDKHEDWSKFGFKFSDTSTYEDLSGFYREVEQQGYKLSFARVSVSYINQLVEEGKMYLFQIYNKDFSEYSKGTPNMHTLYWKALFDERNLADVVYKLNGQAEMFYRKKSIENTHPTHPANHPILNKNKDNKKKESLFEYDLIKDRRYTVDKFMFHVPITMNFKSVGSENINQDVKAYLRHADDMHIIGIDRGERHLLYLVVIDLQGNIKEQFSLNEIVNDYNGNTYHTNYHDLLDVREDERLKARQSWQTIENIKELKEGYLSQVIHKITQLMVKYHAIVVLEDLNMGFMRGRQKVEKQVYQKFEKMLIEKLNYLVDKKADASVSGGLLNAYQLTSKFDSFQKLRKQSGFLFYIPAWNTSKIDPITGFVNLLDTRYQNVEKAKAFFSKFDAIRYNKDKEWFEFDLDYDKFGRKAEGTRTKWTLCTRGMRIDTFRNKEKNSQWDNQEIDLTAEMKSLLEHYYIDIQGNLKEAICTQTDKAFFTGLLHILKLTLQMRNSITGTQTDYLVSPVADENGIFYDSRSCGDSLPENADANGAYNIARKGLMLIEKIKNAEDLDTIKFDISNKAWLNFAQQKPYKNG |
| 547 | Cas12 | MLENEQEAEKIKNKLDDIMNIYHWIKIFLVDEEIEKDMDFYSEIEDIYEELSPLVSLYNRVRNYVTQKPYSQEKMKLNFGSPTLADGWSKSKEFSNNAIIMLKDGKYYIGIFNIRNKPNKEVIEGRNNRINDSDYKKMVYRLLPGANKMLPKVMFSKKGIEYYNPSQYILSGYNSKKHIKSNENFDINFCHDLIDFFKESINKNEEWKNFDFKFSDTESYNDISEFYREVEQQGYKIEWVYISEQDIEQLEKNGQLYIFQIYNKDFAKKSIGNKNLHTMYLENLFSEENLKDVVLKLNGEAEIFFRKSSIKKPIVHKAGSILVNKCIEDETGNKVSFPDDIYNEIYQYMNGMTDVLSERAQNYYEKVKHSVSKQDIVKDYRYTVDKYFIHLPITINFKASSFMPINDIALKYIAKRDDIHIIGIDRGERNLIYVSVIDLQGNIVYQKNYNVVNGYDYKAKLRETEIQRDNARKNWKEIGKIKQLKEGYLSLVVHEIAQLIVKYNAIVVMEDLNMGFKRGRFKVERQVYQKFENMLINKLNYLVDKNKKVDEDGGLLRGYQLTYVPGQKEHVGKQCGFIFYVPAAYTSKIDPTTGFVSIFNNKVNAKEFVTKFDSIKYNKNMKMFELKFDYNNFETYNITLAKSKWTIYTNGIRLKREYNNGRWNKITEVDLTKEMANTLKKYDIEFENNEEILKSISQLDEKNQRNICNEIKEIIKLIVQLRNSMPDNGSKDNEYDKIISPVLNENNYFYDSSEVCDNSAPENADANGAYCIAMKGLYQVIQIKENWSQDSNPKNILGIKHYEWFDFMQNKRYL |
| 548 | Cas12 | MAKNTIFTQFTGLYPVSKTLRFELKPMGKTLEKIKETGVIENDKKRHNDYFDAKKIIDKYHKYFIDAALSKFSRIDWNPLKEAIEGSLDRSDASKKKLEKTQTEFRKKIAKALTTHDHYKELTASTPKDLFLKVFPDHFGKQPAIDTFDGFSSYFTGFQENRQNIYSDEAISTAIPYRLVHDNFPKFLSNIEVYKTLKDNAPSVLSDAENELKDFLNGKPLANIFELNAYNDVLTQSGIDFFNQVIGGISGEGGEKKTRGINEFSNLYRQQHPEFAQKRLATKMIPLYKQILSDRETKSFILESYSTDSQVQESVKEFFESQILNCDIAGRKVNVLNELTSLIKRIAEFDLGSIYINQEELSNISLELFKSWNTINAVLFKNAENRIGSAEKAANKKKIDAWMKSNEFSIATLNLAIAESDSEEISRVKIESYWNDFEAKVQSILCGDNRRNLDEFLSATFNENNALREDSEIIGKLKAFLDALIEIMHSIKPLISDAENRDLSFYNELMPLYDQLSLVVPLYNKIRNYATQKLTESEKFKLNFDNPTLADGWDQNKEDANTAILLLKNGLYYLGIMNAKNKPKIKDFKTSESEDCYDKMVYKLLPGPNKMLPKVFFSEKGLATFKPPKDILDGYNAGKHKKGDLFDIGFCHQLIDFFKESIAKHPDWKKFDFKFSDTSSYEDISGFYKEVTDQGYKITFSKIPTSQIDEWVNEGKLFLFQIYNKDFAPGAKGSPNLHTLYWKSVFSPENLKDVVVKLNGEAELFYRPSSVKKPYSHKVGEKLVNRIGKDGLPLPESVFGELFRYFNGKLDGELSDEAKRYLDVAVVKDVKHEIVKDRRYTQDKFEFHVPLTLNFKADSKNEYMNERVRHFLKDNPDVNIIGIDRGERHLLYMTLINQKGEILKQKSFNIVESVNYQAKLVQREKERDAARKSWSSVGKIKDLKEGFLSQVIHEITTTMIENNAIVVLEDLNFGFKRGRFCVERQVYQKFEKMLIDKLNYLVFKNKPEGDVGGVLKGYQLAEKFDSFQKLGKQSGFLFYIPAAYTSKIDPTTGFANLFNMTELTSAEKKKDFLSHFDDITYDGKNDRFLFGFDYKNFKCFQTDFIKKWTVYTQGKRIVYDKESKSAKEIFPVEIIKAALAKQNIALTDQLDVLSAINSVEASPKSASFFGNICYAFEKTLQMRNSIPNTDEDYLVSPVMNKRGEFYDSRSCDDALPQNADANGAYHIALKGLYLIKNVFDAGGKDLKISHEDWFKFAQSRNC |
| 549 | Cas12 | MRPVLQLTDTEDKLSQNKPAVGKIKALLDAFKDLQHFIKPLLGSGEENEKDELFYGAFQLIWDELDTVTPLYNKVRNWLTRKPYSTEKIKLNFDNAQLLGGWDVNKEPDCTGVLLRKDGFYYLGIMNKKSNRIFDADVTPADGICYEKIDYKLLPGANKMLPKVFFSKSRIDEFAPSEAILSSYKRGTHKKGADFSLSDCHRLIDFFKASINKHEDWSKFGFQFSDTKTYEDISGFYREVEQQGYMLSSHQVSEAYINQMVEEGKLFLFRIWNKDFSEYSKGTPNMHTLYWRMLFDERNLADVVYKLNGQAEVFYRKASIKAENQIMHPAHHPIENKNTLNEKRSSTFDYDLVKDRRYTVDKFQFHVPITINFKAIGQTNVNPIVHETIRRGGFTHVIGIDRGERHLLYLSLIDLKGHIVKQMTLNEIINEYNGLAHKTNYYDLLVKREGERTTARRSWDTIENIKELKEGYLSQVIHIISKMMVEYNAIVVLEDLNMGFMRGRQKIERQVYEKFEKMLIDKLNCYIDKQADSQSEGGLLHPIQLANKFESFRKLGKQSGCLFYIPAWNTSKIDPVTGFVNLFDTRYETREKAKLFFSHFQRICFNAEKDWFEFSFDYNDFTTKAEGTRTQWTLCSYGTRIRNFRNPLQNHQWDDEEIVLTEAFKALFDKYDIDIHANLKEAINAQTDAQFFKDLMGLMKLLLQMRNSKTNSEVDYLLSPVADEHGRFFDSRAGAGSLPDNADANGAYNIARKGLWVIRKIQETPEGEKLSLAITNKEWLEFAQTKPYLND |
| 550 | Cas12 | MNLNTYFSQFTGLYPVSKTLRFELKPMGKTLEKIKETGIIENDKKRHNDYFDAKKIIDKYHKYFIDAALSKFPCIDWNPLKEAIERSLDRSDASKKKLEKTQTEFRKKIAKALTTHGHYKELTASTPKDLFLKVFPDHFGKQPAIDTFDGFSSYFTGFQENRQNIYSDEAISTAIPYRLVHDNFPKFLSNIEVYNILKDNAPSVLSDAENELKDFLNGKPLANIFELNAYNDVLTQSGIDFFNQVIGGFSGEGGEKKTRGINEFSNLYRQQHPEFAQKRLATKMIPLYKQILSDRETKSFILESYSTDSQVQESVKEFFESQILNCDIAGRKVNVLKELSSLIKRITEFDLGSIYVNQEELSSISLELFKSWNTINAILFKNAENRIGSAEKAANKKKIDAWMKSNEFSIATLNLAIAESDSEEISRVKIESYWNNFEAKVQSILCGDNRRNLDEFISATFNENNALREDSKVIEKLKAFLDALIEIMHSIKPLISDAENRDLSFYNELMPLYDQLSLVVPLYNKIRNYATQKLTESEKFKLNFDNPTLADGWDQNKEEANTAILLLKNGLYYLGIMNAKNKPKIKDFKTSESEDCYDKMVYKLLPGPNKMLPKVFFSEKGLATFKPPKDILDGYNAGKHKKGDLFDIGFCHQLIDFFKESIAKHPDWKKFDFKFSDTSSYEDISGFYKEVTDQGYKITFSKIPTPQIDEWVNEGKLFLFQIYNKDFAPGAKGSPNLHTLYWKSVFSPENLKDVVVKLNGEAELFYRPSSVKKPYSHKVGEKLVNRIGKDGLPLPESVFGELFRYFNGKLDGELSDEAKRYLDVAVVKDVKHEIVKDRRYTQDKFEFHVPLTLNFKADSKNEYMNERVRHFLKDNPDVNIIGIDRGERHLLYMTLINQKGEILKQKSFNIVESVNYQAKLVQREKERDTARRSWSSVGKIKDLKEGFLSQVIHEITTTMIENNAIVVLEDLNFGFKRGRFCVERQVYQKFEKMLIDKLNYLVFKNKPEGDVGGVLKGYQLAEKFDSFQKLGKQSGFLFYIPAAYTSKIDPTTGFANLFNMTELTSAEKKKEFLSHFEDITYDGKNDRFLFSFDYKKFKCFQTDYIKKWTVYSQGKRIVYDKESKSAKAISPVEIIKAALAKQNIALTDQLDVLSAINSVEASRETASFFGDICYAFEKTLQMRNSIPNTDEDYLVSPVMNKKGEFYDSRSCGDSLPKNADANGAYHIALKGLYLIKNVFDAGGKDLKISHEDWFKFAQSRNR |
| 551 | Cas12 | MQTLFENFTNQYPVSKTLRFELIPQGKTKDFIEQKGLLKKDEDRAEKYKKVKNIIDEYHKDFIEKSLNGLKLDGLEEYKTLYLKQEKDDKDKKAFDKEKENLRKQIANAFRNNEKFKTLFAKELIKNDLMSFACEEDKKNVKEFEAFTTYFTGFHQNRANMYVADEKRTAIASRLIHENLPKFIDNIKIFEKMKKEAPELLSPFNQTLKDMKDVIKGTTLEEIFSLDYFNKTLTQSGIDIYNSVIGGRTPEEGKTKIKGLNEYINTDFNQKQTDKKKRQPKFKQLYKQILSDRQSLSFIAEAFKNDTEILEAIEKFYVNELLHFSNEGKSTNVLDAIKNAVSNLESFNLTKIYFRSGTSLTDVSRKVFGEWSIINRALDNYYATTYPIKPREKSEKYEERKEKWLKQDFNVSLIQTAIDEYDNETVKGKNSGKVIVDYFAKFCDDKETDLIQKVNEGYIAVKDLLNTPYPENEKLGSNKDQVKQIKAFMDSIMDIMHFVRPLSLKDTDKEKDETFYSLFTPLYDHLTQTIALYNKVRNYLTQKPYSTEKIKLNFENSTLLGGWDLNKETDNTAIILRKENLYYLGIMDKRHNRIFRNVPKADKKDSCYEKMVYKLLPGANKMLPKVFFSQSRIQEFTPSAKLLENYENETHKKGDNFNLNHCHQLIDFFKDSINKHEDWKNFDFRFSATSTYADLSGFYHEVEHQGYKISFQSIADSFIDDLVNEGKLYLFQIYNKDFSPFSKGKPNLHTLYWKMLFDENNLKDVVYKLNGEAEVFYRKKSIAEKNTTIHKANESIINKNPDNPKATSTFNYDIVKDKRYTIDKFQFHVPITMNFKAEGIFNMNQRVNQFLKANPDINIIGIDRGERHLLYYTLINQKGKILKQDTLNVIANEKQKVDYHNLLDKKEGDRATARQEWGVIETIKELKEGYLSQVIHKLTDLMIENNAIIVMEDLNFGFKRGRQKVEKQVYQKFEKMLIDKLNYLVDKNKKANELGGLLNAFQLANKFESFQKMGKQNGFIFYVPAWNTSKTDPATGFIDFLKPRYENLKQAKDFFEKFDSIRLNSKADYFEFAFDFKNFTGKADGGRTKWTVCTTNEDRYAWNRALNNNRGSQEKYDITAELKSLFDGKVDYKSGKDLKQQIASQELADFFRTLMKYLSVTLSLRHNNGEKGETEQDYILSPVADSMGKFFDSRKAGDDMPKNADANGAYHIALKGLWCLEQISKTDDLKKVKLAISNKEWLEFMQTLKG |
| 552 | Cas12 | MIDNTKEKKEGSVFDGFTRKYQLSKTLRFELRPILNTPKMLDDEQVIKNDETRRKKYEAVKPWFDQLHREFIEDALKSFKFKNLAIYQDTFQTWQKDRKSKQKKDTLVKIEVGLREEIVRRFEEVANIWVRSEQYKLLGIKKEGLGMLFEAGVFRLLKERFKNEKDTTVDGNNIFDEWTRWTGYFKKFFETRKNFYKSDDTSTAIAYRVINQNLRRFCENIQIFEKISEKIEFSEVEKSFDISCAGIFSLAYYNACLLQGGIDTYNKIIGGEVDEKDKKIPGINELINKYRQDNSGEKIPFLKQLDKQIHSAKEAFIESIETNKELVGKLKTFYENAEVKIQSFRNLIADIVTDYSGYDIDKIYLTKEAVSHNASRWFASFESFERDLFAVVAEKQNKLVYELLRTHKNDSKISDKDGKFSFPDFIKCSHIKRALEKQEGRIWKGEYYEDIVDFEKIKDVFTQFLCVFKFELEQQFFRKTTSAQTGEQTKIGYEIFVTKINELITRENPVIDLEEKIAIKNFADATLLIYQIAKYFAVEKRRGWLDNYDLDDRFYKSSDIGYLNFYRDAFEQIVRPYNLFRNYLTKKPYNTNKWVLSFENPTLADGWDKNKEKTNAAVILRKDGRYYLGIIKEDCKSLFADRYSKEMSEGIESGSFQKMAYKFFPEASKMIPKCSTQTKNVKEHFRKSSSDYNLFHEKDYKISVAITKNIYELNNVFYRKDNIEESFVPKNDFEKKLGVKKFQRQYLEISRDNNGYKQALAQWIEFCIRFLKAYKSTTIFDYSRLREAKEYESLDAFYQDINALTYNISFVPISEQYIKEKNDNGELFLFEIYNKDWSLGPMDKNRKRTKNLHTLYFEQLFSKENEQENFLFQLNGEAELFFRPKTEEKRLGYKVWDAGEKKWVKAKEKEDGAVIDRKRYAKDIILFHCPITLNRVSESKTKREMDVEIREVLSSTPGVHIIGVDRGEKHLAYYSVIDQNGKIIETDTLNSIGKDGRGKPVEYASKLEKRAQEREASRRDWEEVEAIKDLKKGYISQVIRNLADLIIKHNAIIVFEDLNMRFKQIRGGIEKSAYQQLERALIDKLSFLVKKGEEDPKQTGHILRAYQLAAPVIAFKDMGKQTGLIFYTQAGYTSKTCPECGYRRNIKCLFENIEQAKTLIENLESINYNKKEDVFQISYSLEKLSSKDQKKEKKVSNELYAKTLKKDIFILTTKNALRYKWYDRYSEKAKVAKRGIDEYKGEVNESETKKGVVKEFNLTEYLKGLLKTYEIDYEHGGIREQILSVARGREFYKDFLYALFLLTETRHSISGRNTDYIQCPECEFDSRKGFKDIKEFNGDANGAYNIARKGIMILEKIKQFKKDNDGNLEKMGWGDLSISIEEWDKFTQKE |
| 553 | Cas12 | MKNFQDFTNLYELSKTLRFELKPIGGTKKLIEEKNILKLDKKKRENYEKVKPYFNKIHQEFINFALRNPNFDFSQFEEKYLNWLKDKKNKDLLKEKESIDKIFLEKIGKLFENSVKDFLKENGFESIVKEEDQNLKFFRRKEIFEVLQEKYGSELETQMVNKDGEIKSIFNGWEKWLGYFDKFFNTRDNFYKTDGTSTAIATRIIKDNLKIFLENIVAFGKIKNKKIDFSEVEKNFSVSIDTFFEINNFNNCFLQDGIDFYNKVIGGETLENGEKLKGLNEIINKYRQDTGEKIPYFKKLQKQILSEKDGVFIDKIEDDGGFYEVLKNFYKNAAEKEGFLKNIFENFYTISDKNLEKIYFNKIAFNTISHKFGSALEFERILYEEMKKEKADGIKFEKKENKYKFPDFIQIIFIKRSLENYDSENLFWKERYYKSEENVDGFLEKNNNNLWGQFCKILNFEFLNILKRRIIDEAGEEYEVGFEISKNILGEKLENFELNQENKGIIKDFADYSLALYSFGKYFAVEKGRNWDLNIDISDDFYGGEDGYIEKFYNTGYDEIVKPYNLMRNYISKKPWEDNKKWKINFETSSLLSGWDKNLESNGSYIFQKGNKYYLGIINGSKPAKEILEKLYSGDGEKIKRFIYDFQKPDNKNTPRMFIRSKKDSFSPAVEKYNLPINDILEIYDNGLFKTENKGNPNYKESLRKLIDYFKLGFSRHESFKHFNFVWKDSKSYENIADFYRDVEKSCYKIDFEFLNFEELKKLTFEKHLYLFQIYNKDFELDESLQEKGYNFKGEGQKNIHTKYFEALFLEENISRKSGAVFKLSGGGEVFFRKKSIKAKKEKRNSVEVIKNKRYTECKYFLHFPIQVNFKEEISGNFNQEINKFLANNPDINVIGIDRGEKHLAYFSVINQKGEILESGSFNKIENYNKNGEKLLFPEREIKEIHKDGSLIDLELVETGRKVDYVDYKLLLEYKERKRLLQRQSWKEVEQIKDLKKGYISALVRKIADLIIKHNAIVIFEDLNFRFKQIRGGIEKSIYQQLEKALIDKLNFLVNKNEINLEKAGSILKAYQLTVPVDSLKEIGKQTGVIFYTEAAYTSKIDPITGWRPNLYLKKNNSKINKENILKFDNIVFNSKENRFEFTYDLKKFFGKDSKFPAKTVNTVCSCVERFKWNRNLNNNKGGYIHYENLTDGKLANKEQKEDEFSNFKELFEKYFIDINGNILEQIKNLDTKNNEKFFSSFIDLFTLVCQIRNTNQNAKGDENDFILSPVEPFFDSRKSQNFGKSLPKNGDENGAFNIARKGLIILNRISENPEKPDLLIFNADWDNFARNI |
| 554 | Cas12 | MSNNFQEFTQKYALSKTLRFELKPVGKTKEILEKEMPMYQIINADKNIKAKYIQTKPFFDQLHRDFIKEAFENVELSGLSDFFENWKIYKQDKKANEKIYKKSAENLRKEVVSFLNAKGKDWAEKYHSSGLKKADIEILFEEGIFKVLEIRYGTDTNSFITNATTGEITSIFQGWKGFTGYFLKFWNTRENYYKTDGTSTAIATRIVDQNLPGYLENLEIFEKMKGKIDFESVRGDFSDFEKIGTVEYYSTCLLQEGIDGYNRIIGGYTYENGEKIKGINEIINLYRQTHKDEKVPFLKTLDKQIGSEKIAFMETIDTPEEFRKIFEEFVLKSSEKVVLLKQCLNHLFENELTDGVFLSKESLNTISHKWIDIGNMKLFHESLFTILKKEGAKYDSKEDEYKFPDFIRISDIKTALVKITTESFFWKNRYLYEKDENPTGFLTSDNSLWEGFIQIFSHEFSSLFERTEKDEEGKDIQWGYDISLLNIQKLLENNEYNPNDEKNKIIIKSFADDILRIYQMGKYFALEKKRQWNPDNLEIGEFYSHPEIGYDKFYFDSYKIIVQGYNDIRNYLTKNPWSEEKWKLNFENPTLANGWDKNKETDNSCIFLKRDNKFFLALMSRGNNQVFDERNIQKFAQNIEQGKYEKMVYKYMKDVALGIPKATTQLNAVQEHFFQSDKDYIITKGGSSIGEFIKPLRVTKRIFELNNRIYPKDNLGISFLRNQVNKKEQKNYIKIFQKEFITLGGDEVVYKKAVHDWIDFCKEYTKSYPSCAYFDYSGLKDTKEYSSIDEFYNDLDSFGYQISWQDISSSYIDELVESGKLYLFEIYNQDFSNGKTGAKNLHTLYFEHIFSKENQEVNFPLKLNGQAELFFRPKSIEAKGENRKFNREIIAKKRYTEDKIFFHVPLTLNRTEGDIYGFNTEINNFLAHNPDINIIGIDRGEKHLAYYSVIDQKGNIIESDSLNTVNEINYGEKLTDTAEKRKQARQDWQAVEGIKNLKKGYISAVVHKLTDLIIKYNAIVIFEDLNMRFKQIRGGIEKSVYQQLEKALIEKLNYLVEKGEINPEKAGHLLNAYQLTAPFETFKDMGKQTGIVFYTQAAYTSKIDPVTGWRPHLYLKYSSAEQVKKEIAKFSNIIWNNTEKRFDFMYDIRNFSTQKEYPKNNIWTVCSSVERYRWDKTLNQNKGDYVHYKSITPEFEKLFSDFQIDGTKNILEQINRMETKGNEKFFKSFIFFFGLICQIRNTNKADSDENKQDFILSPVVPFFDSRDSENTKNGLPRNGDENGAYNIARKGLIILQKINEFSDENGNCDKLGWKELSISQVDWDNYIKT |
| 555 | Cas12 | MEYDEHISLIESEEKADEMKKRLDMYMNMYHWAKAFIVDEVLDRDEMFYSDIDDIYNILENIVPLYNRVRNYVTQKPYTSKKIKLNFQSPTLANGWSQSKEFDNNAIILIRDNKYYLAIFNAKNKPDKKIIQGNSDKKNDNDYKKMVYNLLPGANKMLPKVFLSKKGIETFKPSDYIISGYNAHKHIKTGENFDISFCRDLIDYFKNSIEKHAEWRKYEFKFSATDSYNDISEFYREVEMQGYRIDWTYISEADINKLDEEGKIYLFQIYNKDFAENSTGKENLHTMYFKNIFSEENLKNIVIKLNGQAELFYRKASVKNPVKHKKDSVLVNKTYKNQLDNGDVVRIPIPDDIYNEIYKMYNGYIKESDLSGAAKEYLDKVEVRTAQKEIVKDYRYTVDKYFIHTPITINYKVTARNNVNDMAVKYIAQNDDIHVIGIDRGERNLIYISVIDSHGNIVKQKSYNILNNYDYKKKLVEKEKTREYARKNWKSIGNIKELKEGYISGVVHEIAMLMVEYNAIIAMEDLNYGFKRGRFKVERQVYQKFESMLINKLNYFASKGKSVDEPGGLLKGYQLTYVPDNIKNLGKQCGVIFYVPAAFTSKIDPSTGFISAFNFKSISTNASRKQFFMQFDEIRYCAEKDMFSFGFDYNNFDTYNITMGKTQWTVYTNGERLQSEFNNARRTGKTKSINLTETIKLLLKDNEINYADGHDVRIDMEKMDEDKNSEFFAQLLSLYKLTVQMRNSYTEAEEQEKGISYDKIISPVINDEGEFFDSDNYKESDDKECKMPKDADANGAYCIALKGLYEVLKIKSEWTEDGFDRNCLKLPHAEWLDFIQNKRYE |
| 556 | Cas12 | MFGNWGVIQNAVMQNIKRVAPARKHKESEEDYEKRIAGIFKKADSFSISYINDCLNEADPNNAYFVENYFATFGAVNTPTMQRENLFALVQNAYTEITALLHSDYPTEKNLAQDKANVAKIKALLDAIKSLQHFVKPLLGKGDESDKDERFYGELASLWAELDTMTPLYNMIRNYMTRKPYSQKKIKLNFENPQLLGGWDANKEKDYATIILRRNGLYYLAIMNKDSKKLLGKAMPSDGECYEKMVYKLLPGANKMLPKVFFAKSRMEDFKPSKELVEKYNNGTHKKGKNFNIQDCHNLIDYFKQSIDKHEDWSKFGFKFSDTSTYEDLSGFYREVEQQGYKLSFARVSVSYINQLVEEGKMYLFQIYNKDFSEYSKGTPNMHTLYWKALFDERNLADVVYKLNGQAEMFYRKKSIENTHPTHPANHPILNKNKDNNKKESLFEYDLIKDRRYTVDKFMFHVPITMNFKSSGSENINQDVKAYLCHADDMHIIGIDRGERHLLYLVVIDLQGNIKEQFSLNEIVNDYNGNTYHTNYHDLLDVREDERLKARQSWQTIENIKELKEGYLSQVIHKITQLMVKYHAIVVLEDLNMGFMRGRQKVEKQVYQKFEKMLIEKLNYLVDKKADASVSGGLLNAYQLTSKFDSFQKLGKQSGFLFYIPAWNTSKIDPVTGFVNLLDTRYQNVEKAKSFFSKFDAIRYNKDKEWFEFNLDYDKFGKKAEGTRTKWTLCTRGMRIDTFRNKEKNSQWDNQEVDLTAEMKSLLEHYYIDIHSNLKDAISAQTDKAFFTGLLHILKLTLQMRNSITGTETDYLVSPVVDENGIFYDSRSCGDELPENADANGAYNIARKGLMMIEQIKDAKDLDNLKFDISNKAWLNFAQQKPYKNG |
| 557 | Cas12 | MSNLYSNLHNLYQVQKTLRFELKPQGKTKENMEKVGILKADEHRAEIYSKVKKYCDEYHKLFIDKSLSNIELNGIDRYYELYSINNRDDKQKEELDQLEASLRKQISDAFKKSAEYKGLFQKDIITSYLVTMYKENQEKMQDIGEFNRFTTYFTGYNKNRENMYSEEDKSTAISYRLINENLPTFIDNIKIYKKIVSLMPEDIEKIYKDLEEYIQVDSIDEIFNISYYNDVLTQRGIECYNILISGRTKNDGDKIKGLNEYINEFNQTHNEKIPKLQELYKQILSDAESASFKIDVIKNDKELMNLIEVYYANILPILNKIEDLFTRISNYNLELILVNNDGTLSTLSNMVFNEWSYIKGAISEKYDEEYSGKEKYGTEKYAQKKQEYLKKQKIYSLKFLNDCIGNNAICEYLKNYIIQNKNIETIKEDYNEVQNIKVEDDTKELIKDEKSIEKIKKFLDDVKSLQEFVKLVIPKDRTVEKDAKFYSELTPYYEKIKEIIPLYNKVRNYVTQKPYSTEKIKLNFECPTLLKGWDANKEEANLGVILLKEGKYYLGIINPYCKKIFEVDEKDSNEQNNYKKMEYKLLPGPNKMLPKVFFSNSRIEEFNPSKELQEKYNKGYHKKGKDFDINFCHELIDFYKQSVNKHEDWKKFNFKFKDTSEYNDISEFYREVEKQGYKIEYTEYSEKYINELVDRGELYLFQIYNKDFSEYSKGKENLHTLYWKAVFDPDNIMNPVYKLNGNAEVFYRKKSLEMKVTHPANQPIANKNISTIEAGRSTSTFKYDLIKDKRYTMDKFQFHVPITMNFKSERLFNINQIVNKYLKYNDDIHVIGIDRGERNLLYVCVIDKNEKIVYQKSLNEIVSEYNNNRYTTDYHGLLDRKEKEREIAREDWKNIENIKELKEGYMSQVIHILVELMKKYNAIIVIEDLNKGFKNSRIKVEKQVYQKFEKMFIDKLNYLVFKDEDKMNEGGVLNAYQLTNKFESFTKLGKQSGILYYIPAWCTSKIDPTTGFINRFYLKYENFDKSKEFVNRIDDIRYNEKENLFEFDIDYSKFTDRLNDTKNKWTLCSYGERILTQKNANGEWLDRRIQLSIEFKKLFEKYGINLNNIKDSILKLDKDNLEFYKGNGESLGFIQLFKLMVQMRNSLTGKEEDNLISPVKNQHGKFFNTSEKIEGLPIDADANGAYNIARKGFMLVEQMKNVEDEKLNKIKYNITEKEWLNYVQNRGM |
| 558 | Cas12 | MARIFEDFKRLYPLSKTLRFEAKPIGATLDNIVKSGLLDEDEHRAESYVKVKKLIDEYHKVFIDRVLDNGCLPLKNEGHNNSLTEYYDSYVSKSQNEDAKKAFEENQQNLRSIIAKKLTEDKAYANLFGKNLIESYKDKTDKTKIIDSDLIQFINTAESTQLNSMSQDEAKELVKEFWGFTTYFVGFFDNCKNMYTAEEKSTGIAYRLINENLPKFIDNMEAFKKAIARPEIQANMEELYSNFSEYLNVESIQEMFLLDYYNMLLTQKQIDVYNAIIGGKTDDEHDVKIKGINEYINLYNQQHKDDKLPKLKALFKQILSDRNAISWLPEEFNSDQEVLNAIKDCYERLSENVLGDKVLKSLLGSLADYSLDGIFIRNDLQLTDISQKMFGNWGVIQNAIMQNIKRVAPARKHKESEEDYEKRIAGIFKKADSFSISYINDCLNEADPNNAYFVENYFATFGAVNTPTMQRENLFALVQNAYTEVASLLHSYYPAEKKLAQDKANVAKIKALLDAIKSLQHFVKPLLGKGDESDKDERFYGELASLWAELDTVTPLYNMIRNYITRKPYSQKKIKLNFENPQLLGGWDANKEKDYATIILRRNGLYYLAIMDKDSRKLLGKAMPSDGECYEKMVYKLLPGANKMLPKVFFAKSRMDDFKPSKELIEKYNNGTHKKGKNFDIQDCHNLIDYFKQSIDKHEDWSKFGFNFSDTSTYEDLSGFYREVEQQGYKLSFARVSVSYINQLVEEGKMYLFQIYNKDFSEYSKGTPNMHTLYWKALFDERNLADVVYKLNGQAEMFYRKKSIENTHPTHPANHPILNKNKDNKKKESLFEYDLIKDRRYTVDKFMFHVPITMNFKSVGSENINQGVKEYLHHADDMHIIGIDRGERHLLYLVVIDLQGNIKEQYSLNEIVNEYNGNTYHTNYHDLLDAREDERLKARQSWQTIENIKELKEGYLSQVIHKITQLMVKYHAIVVLEDLNMGFMRGRQKVEKQVYQKFEKMLIDKLNYLVDKKADASVSGGLLNAYQLTSKFDSFQKLGKQSGFLFYIPAWNTSKIDPVTGFVNLLDARYQNVEKAKAFFSKFDAIRYKRIRTGLSLISTMTSLVKKQKVQGLSGFYAPEECVLILSEIKKKTHNGIIRKLT |
| 559 | Cas12 | MISLNYFQNQYAVAKTLCLELRPIEKTMEYIISSGILKEDEHRNESYKLVKKIIDDYHKAYIELSLSRFELKITSCSKNDALEDFYCQYLANSQEEKDKNIFKKTQDNLRKQIAKHLTQGEAYKRIDKKELIQEDLLEFVAADPDAANKKILINEFRDFTTYFTGFYENRKNMYSEEAQSTAIAYRIIHENLPKFIDNMGTFKQLMQSSITDILPQIFDNFKKDLEVSSIQEIFDLNYFNKVLTQKQIDIYNAIIGGKSLNENSRIQGLNEYINLYNQQHKENKLPLLKLLFKQILSDRNSLSWLPEAFETDKQVLHAVRKCYANLKESVLHEAGLVQLLSSLPSYDSTRIYIRNDQALTTISQKLFGDWGIIPHAIKERLKKDISAKRKETEEAYLERIEKAFKQADSYTIAYINDSLKEIGVDKKNIEDYFIHLGAICTEGQEQENILQRIASAYSQAQPLLEEKVPVHKNLMQDKDSVELIKSLLDELKNLQHFIKPLLGKGSESDKDERFYGEFVGLWNELDQITTLYNKVRNYVTRKPYSIEKFKINFQNATLLKGWDRNKERDNTSIILRKNGLYYLAIMRKEYNKVFEKYPAGTEENCYEKMEYKLLPGANKMLPKVFFSKSRINEFNPSPQLLQNYQMGTHKKGDQFKKEDCHALIDFFKTSIEKHEDWKNFNFQFSPTSVYEDMSGFYREVEQQGYKLVFRSIDAEYIDKLVEEGKIFLFQIYNKDFSPFSKGTPNLHTLYWKMLFDERNLNNVVYKLNGEAEIFFRKKSITYTHPTHPAEIPIKNKNVQNKKKESVFQYDLIKNHRFTIDSFQFHVPITMNFKNAGLSNLNEQVYTYLRENKDAHIIGIDRGERHLLYLVVIDRYGRIVKQFSLNEIVNEYHGNTYTTNYHDLLDKREEARQQARQSWQSIENIKELKEGYLSQVVHKIANLMVEYHAIVVLEDLNIGFMRGRQKVEKQVYQKFEKMLIDKLNYLVDKKKAPEADGGLLKAFQLTNQFESFQKLGKQSGFLFYVPAWNTSKIDPCTGFTNLLDTRYENIAKAQKFFRTFDAIRYNAVKDYFELELDYDKFHKRAEGTQTKWTLCTYGTRIKTFRNPENNNQWDNVEINLTEEFKKLFKQFGINLSGDLQQAICAQTEKSFFESLLRLLKLTLQMRNSITGTDVDYLLSPVQNAEGYFYDSRKGDKSLPANADANGAYNIARKGLWVIQQIKQTPQGQKAKLSISNKEWLKFAQEKPYLKD |
| 560 | Cas12 | MKTFENFTNLYSLPRTLRFELKPLYKTKELIDSKQELFPKDKRIDEIYQNIIKPCLNELHSDFIEKSMENKDFQNIPDNILKIYSNEKNIDDFKNIEKDLIKQINWFLKSNKTFFAENYSDLLGKNSIDIIIKVFWEKIYKKDDSWKIFLYNDLLWKSYEELINIYFKWFSTYLSNFNKNRENLYDKKNEAKVWSVSGRTIWENFPRFLQNCINFRDKLEKLNLSIEQKDIFITNNFWKCISQKQIDYYNKIIWQINSKTNEFNQKNGLKWNKKLPKLLLLHKQILWKSENENILNFINNIIQTDFELEQEIKIINKDIFQRIDFIKKSIVSNIEDFELEKIFIKKNRLKDISSLLMDNYSVLEKLLPEFNEEWKIIKENELVNLSKIKKSFENIDLKDLKNIFKKEYFDESKDWFKLFLNWIYNHFSDLENNIKHTHKLVQDKLISWNFSENIQKSEKNINLRDEIFVSSKWLLKAYLDSILALDRFVHMFDYWEQKDFDSNFYNNIEEYSINFSPFKTYNAVRNYLTKKNYSTDKIKLNFDYPDFLGSNSLWKYAFIYKDSKWFYYLWVLDHSNSQSKYKPQILKNNTEFYQLEYKQIKFNTLAWKWYIRDFWVKYSEDENCIINLKTLIKKQYLERYPVLKEIVDFQTDDKKIFDAKVKTILEQAYSINFVNIDKNYILEENNNWNLHFFQIYNKDFSENKKINSMENLHTMYFKALFEKENFNWWACFKLNSQWAEIFFREKSINEKKVKDLKTRNENAIEKKRYTENKVFLHLPITLNFINKWYSKYSFWYINDSVKKYIKENKISIIWIDRWEKNLIYFSMINENLEIIELKSLNSLILKVSDLEEKEVNYFEKLSKKEWNRNKERKDWDEIETIKELKEWYISQIVDNLVKLIVKHNAIVVMEDLNSGFKRWRQKIEKQIYQKFELALAKKLNFTVDKNKKHDELWWIYKAYQLTPQIENFQDIYSQTWIIFYTQAAYTSVTCPNCSFRKNIYQKYENESKFKEFFKKYILEIKFEDNCFIIKYKIDEKIDKKKNKLKKLEFQVNTKNQIRLKFEKSLKWKWWETKEFNITEKFKEIFEKHKLDLWNLKEELLAWNWEIQLYKDFMFYFNLLLQLRNSKENDNWWYISCPSCWFHSGNWFQWFSYNWDANWAYNIARKWRIILDKIKKDEKNLWITNVEWDNYYQK |
| 561 | Cas12 | MAVRSVKLKLLVPRDGSAESVRKRKALWATHQFVNDAAAAYAELLLEMRQEDVCRGTDDHGKDVIEPAAHWQAKLRARLAAKQLPPVAVAEALPLLKAFYGSRLIKSFVANDKGVAGTGNATDLNTWLSGLVDPASVAGEKTELRKQLLAELPLCEAADADFEGAARKMLAKSDAREALLEGPGTGVGWPAAYNANPTDSVWLDMLHKAAAKARLELADTTVSELKKLGVFPLLQAASSNRVFGSGVLNPFERMAAAQAAAALLPWETKRHEMRKRRDKFADQLNQWDTEFGASHATALAAIRAFEAEESERARRESLGNEGTGYRIGGRELRDAWTLLRDWLKGHSTATAAAREDKVRELQAKQGRSFGSHRLLSWLAKPAQQWLADHSAGDVVTRIAVRNARQRKLDTARTLPIWTGADAVKHPRFANFDPPNNTNQPGFDLRAGTQKGRLTLRLSLLTERADGLLLAQDHDFQLVPSRQMAEIVLHKDGKERALSWQSQDGIGRQVGDVGGSALLFSRDHAECLLERKQITRLERGAWPAALPVWFKLSLDIGAEHKALLKQRFKWGVWLNSALVTRNAKDAKGVPPPVGTRVLAVDLGLRSAATVSVWQVVDAATPVVAGKWRVPLSDTLSAVHERSAMLALPGEHVDAGVLAARRAANEKLAGLLAATSHLSTVFKLGRAEQGDRRRELLERLGEGDDRRARAAVATTAAERDGLRAVLGATQDAWAGAVAAVWRRLETDLAGAIAAYRKQQREDVQLRREARHGPGASQLPKQAAAERLLGGKSAWQIEYKERVRKLLTRWIMRQRPGDTAVRRLARKDLGKYCGGLLDHLTALKEDRAKTTADLIVQAARGRVRAHKDAHGRQQDRELWLAKYAPCDLIVMEDLGRYRFATDRPPSENRQLMQWTHREVFRLVQMQAEVEGIQVLETGAEFSSKFDARTWAPGVRCEPITKLWVERYRNGEMPWLADKADEWRREGIELAQLVPGQLLPTGSGEQFVAVSATGGLRVRHADLNAAQCIALRALTGHGTAFRLTARRLGDVFVSAKGLGKRPQGALWREFGSALPPAVVVLRPAGEVRYALRPFASARDAAAALGLQLGALRNVDATDAESDAEDGDLAELLAGADPDRATFFRDPSGDVHGGAWVQAKVFWAEVRRHVRLGLQAQGLLPAAARSSEPRQMQLPLAGALPGDDIPL |
| 562 | Cas12 | MLDKFASLYPVTKTLRFRLLPQGRTEENMQVAKVLENDLERSEAAAVVKGLIKKYHLQFISDTLSGSTLSWQALTETLDKFKADHTATAELDSALAAYRCKLAELFTKSPKYKVMATPVSIIKEILKTETDPENIAALNKLNGYTYIIFDYVSTRMLTYSADAKATSLAYRLVDENYLRFYQDISAAAEISAVLEEAGFDNAEVEAFIRTDYNTCLTSEGIASFNAAAGSINQFVNVLLQQNPVLQSEPALRRHLQPLYKMLLDEAESKIIKFEDYGQLRDAVENFRRNFQDLPQSLIDIFAGRYDYSKIYVGYKYLNEASSQIAGGYNWKLLENALEDFYSKPYLVNGKLPVKYKTVVNKKMNQLAYSFTELQEALDAGDSGSSITDLFGKYAELHAAYAAADGNVFYKEYDRKSIASLKNYLDAVNAIARFIKIFAAPEVYVKDEGFYGIVDGAADKLRDFDLLYNMVRNYITKKPYKKSKVALTFNSSSFGRGWDENKIYDELTTIFTYNGKYYLGVINKNDKPDLAAAVSKDEGGYKRMVYKTFDIVKQLPRLSFTKAVKAHFAESDEDFIFDGPKFAKPLRVPKEIYLQSFTDNGDKLADSAKKYTKAYLDMSGDYKGYYEAIIKRIDYTKEFLSAYKSTSIYDLAFLKPAGKAAGSLCWTRHI |
| 563 | Cas12 | MKTFENFTNLYSLPRTLRFELKPLYKTKELIDSKQELFPKDKRIDEIYQNIIKPCLNELHSDFIEKSMENKDFQNIPDNILKIYSNEKNIDDFKNIEKDLIKQINWFLKSNKTFFAENYSDLLGKNSIDIIIKVFWEKIYKKDDSWKIFLYNDLLWKSYEELINIYFKWFSTYLSNFNKNRENLYDKKNEAKVWSVSGRTIWENFPRFLQNCINFRDKLEKLNLSIEQKDIFITNNFWKCISQKQIDYYNKIIWQINSKTNEFNQKNGLKWNKKLPKLLLLHKQILWKSENENILNFINNIIQTDFELEQEIKIINKDIFQRIDFIKKSIVSNIEDFELEKIFIKKNRLKDISSLLMDNYSVLEKLLPEFNEEWKIIKENELVNLSKIKKSFENIDLKDLKNIFKKEYFDESKDWFKLFLNWIYNHFSDLENNIKHTHKLVQDKLISWNFSENIQKSEKNINLRDEIFVSSKWLLKAYLDSILALDRFVHMFDYWEQKDFDSNFYNNIEEYSINFSPFKTYNAVRNYLTKKNYSTDKIKLNFDYPDFLGSNSLWKYAFIYKDSKWFYYLWVLDHSNSQSKYKPQILKNNTEFYQLEYKQIKFNTLAWKWYIRDFWVKYSEDENCIINLKTLIKKQYLERYPVLKEIVDFQTDDKKIFDAKVKTILEQAYSINFVNIDKNYILEENNNWNLHFFQIYNKDFSENKKINSMENLHTMYFKALFEKENFNWWACFKLNSQWAEIFFREKSINEKKVKDLKTRNENAIEKKRYTENKVFLHLPITLNFINKWYSKYSFWYINDSVKKYIKENKISIIWIDRWEKNLIYFSMINENLEIIELKSLNSLILKVSDLEEKEVNYFEKLSKKEWNRNKERKDWDEIETIKELKEWYISQIVDNLVKLIVKHNAIVVMEDLNSGFKRWRQKIEKQIYQKFELALAKKLNFTVDKNKKHDELWWIYKAYQLTPQIENFQDIYSQTWIIFYTQAAYTSVTCPNCSFRKNIYQKYENESKFKEFFKKYILEIKFEDNCFIIKYKIDEKIDKKKNKLKKLEFQVNTKNQIRLKFEKSLKWKWWETKEFNITEKFKEIFEKHKLDLWNLKEELLAWNWEIQLYKDFMFYFNLLLQLRNSKENDNWWYISCPSCWFHSGNWFQWFSYNWDANWAYNIARKWRIILDKIKKDEKNLWITNVEWDNYYQK |
| 564 | Cas12 | MSFLVPHLPTVVSHRLGGYSSAMDQTPTRLTPAVKNTAAATPKPEVPLTQRAYTLRLRGANDGDQSWREAVWATHEAVNMGAKVFGDWLLTLRGGLDRELADAKVKAGNNNPDRNPTPEERRGRRVLLALSWLSVESAPKKGDAYEKFVIASGKKDSQSIRDEKVVRALREILAKRGVANQDLEGWIVDCEPSLSAAIRDDAVWVNRSAAFDAAQCRVGTSLTREEIWDLLKPFFGSCESYLASLTTDEDSDTEAAATDDKAKDLVQKAGQWLSSRFGTGKGADFAAMSKVYSETAFWAGRASPFRSGAEALRLIAESLKSFCPKSFDADGILGLISGPGYKSATRNIIKAWSKRAGPVTADDLANLSAVAAEDANKCSANTGSKGHRPWSDAILCEVENACGFTYLQPDGPALHSEFAVMLDHAARRVSIGHSWIKRAEAERDRFTKDALRIKEVPDPIRVCLDRFCADRAGTSGAIDGYRIRKRAVSAWKEVITRWGQAACKTAEDRVIAVRETQADPDIDKFGDIQLFEALAVDDAECVWRVNGEVTPQPLIDYAAATDAEAKQKRFKVPAYRHPDPLSHPVFCDFGNSRWKIRFAAHDAVTKLANARTSLDRREADLAKAKERLDKATAPEEQTEGKESLEEAERHLRDARDRVAWLSSMHAFSMRLWGAGRVGDGQRLFWSCKRLTDDMALRQNSGQAPTIAVTRADRLGRAAAGADTADSVDILGVFTEEHWNGRLQAPRAQLDVIAAHVAKNQWDAKAKKMRDRIRWLVSFSAKLQPVGPWIEYSATFPEAALAKPFVSRKGEYAVRHVSNDDRAGLGKLVLSRLPGLRVLSVDLGHRYAAACAVWETVSIAQTNAACDAAGHELPTESDLFLQLSTTDLTGKNRTTIYRRIGADMITDPKTGEKTPHPAPWARLERQFLVKLPGEDIPARKASPAEFDAIRRLEEAFGRTRTADDPLLVRVDELLAATVDSARLALRRHGDAARIAYAFKPSAEKLTPGGGREVMSPEARKAMILDALLLWHGLWHGDRWADVWASQQWDAYIKPELGMDLPPWSETSGEPRCQYRSKVEGLLKRVAESLAARDGAGLHLLWAEQWRTRNAKWLGNTGHLRTLRSLLLPRGLTTSTPAAWNVGGLSLTRIATLKSLYQLHKAYHMRPEPEDPRKNVPAKGEEELRDFGRGMLDVMERLREQRVKQLASRLAEAALGIGRMKASEGKRDRKRPRAQIDQPCHAVVIENLKNYRPEETRTRRENRQLMSWSSSKVKKYLSEACQLNGLHLREVQASYTSRQDSRTGAPGIRCADVPVQDFFTKPWWRRQVSIAVGRVNQGRGDARERFLADLDAKWSAAEKSERITAPPLRIPVNGGELFVSADLHSPAALGLQADLNAAANIGLKALLDPDWPGKWWFVPASLDADGWRVPAAKSCAGAEWVKNWKVGQLGDSYAPNGKPLQPTDDEGVKKAEDGVKLAKASLDDAEQALKAAKKTKRKAEIDVASARAQEAKKNVDDMKKALVAAKKGASAKEIINLWRDPVGVDPADFASGEPWRAYTVYKQRVEYHVINDVLRGRAGPRSDRP |
| 565 | Cas12 | MSITRSIKVKLIVPRDASLEARQLREGLWATHLFVNDGCHYYERLLLEFRQRDVCVGKDDAGKDVIVPAAEWADRLRARLGRNGMVPSHIEAALPIFRELYENMVPSALKAKSGTGQAGRSWHSKLVSPTSRGGEASAARIDVLRPLLPVSGDDPAFEPAARALIEEAGDELLTSTGRCPAWVTAYRKGPEGSAWVEKLRIQLREAVEAGDFDPPSDPQILAAGAVPAAPPLGAGIDALRPLLPLLGGDPAFEPAARALVEDIGDELFTSTGRPPTWVTAHPTWVRAHRKDAECLEAADDFKWVERLRQRLRDDAKAGKFEQPLHERLGALGALPVAKPIGAGRVVSRADLTVFERGAMELAIEHLIGWESAGHRARAQYVERKKRHDDLLQWIEAEAPDALLAVRAYEAARTIHLATLGELGAAPQYTLRLREIRPWRKLREWLLQNPDATIDERRRRLATMQTNDPRGYGGEALAWLAAPERRALVEHPAGDVVTRIAVLNIRKSILDRSRLFPTCTLADPVEHPRFAKFGKPGDKNSAGYALAVDGVRREAIIKILVPRQDGLLVPTDLRVPFAPSGQMRDLRASGLDISYERQDGRGRQAAKLQGGNLMFDRTHFARCGAPGPEALGSVWIKVALDLSSPAASLAMKTATPVRTYLSTAVRGRPESTKYEKAAPPEGFRVLSVHMGLRTAATASMLRFGAPEEGGHEVPVSGLAGETLVAFHERTVTMKLPGEDPDTRTEANRGVAKRELRGLGRGIGCLKAIRRASASATPEDRAEALVIIETHVGQGDRHGWAPAEAVGRLDPHGDPDDWKTACAALYAAVEADLGVAISSWRKAARAGGATGMLGGKSLWAVDHLERSFRFLRSWDLRARPHDGDPRRPRPGYASKLLHHIDGVKDDRVKTTADRIVQAACGRAWIGGPTVKRGTQDVRLPGRWEQRGPRADLILLPDLTHFRFRSDRPRAENSRLMRWAHRQLAIYVRMQAEVEGILVADTGAAFTTRFDAWTGAPGVRCEPVTADHLRGIAKREDYWLARLLREGALKHLRIDPASLRVDDLVPMDHGKILVALDGVDLPGLRILDTDVNASQGLGRRYIEGHGLAYRLPGARVPRGEGEREAAVVHIKGKRLASAMGGTVVVLRASEGPGDITWTAEVYDRPQGARKALGLSLAAFNSIATAAVDDEGPAPENDDEALEEEAEEALGIATGERIVFFRDPSGAVAGGGWLEASAFWGIANRMVTDRLRELGRLG |
| 566 | Cas12 | MATAVDTSTTRAYTLRLSGGNNWRELLWQTHVAVNRGAWVWGDWLLTLRGGLPASLADGDAERRVVLALSWLSVESPASLAPQAHIVAYGSDARDERNRKVTERFRDILRRMGIKQQQEQEWLDACLPALMASIREDAVWVDRSACFAEAQQCYRGLSSEWARKTLFDFLGGEDDYFKPSAKEGASSKAKDFVQKAGRWLSRHWGAGKKSDPRDISTRLGKLAGVDPKAIDGHTGRAALEDLLRTLGSRPAQNADAEKLYRQLKRAVGWKGRPSKGAVALKKIRDAERVPNDLWKEIASTLREEAAVQSSQTSDHAAVPDWRSHWPAEITGLPMPYRVDRDYIWEHGVMLDHALRRVSSAHTWIKRAEAERRRFQQDAAKMGSIPEEARNWLDAFRERRSSSSGATGDYLIRERAINGWDKVVQAWETLGPNSTRDQRIAAARDVQANLDEDEKFGDIQLFAGFGDEHVDDPERCLADDRATCVWRNSSGRADGRILKDYVAATVAEHNQRRFKVPAYRHPDPLRHPVFVDYGKSRWSINYSALTAAQQRRKTTQKLAQAKTDNTRAKLQQQLASTADLRSVTLGVWDGNRIVKISQRWRSKRFWRDLDLDHFGSHPSAAVSRADRLGRVAARQDPGAAVYVAKVFEQQDWNGRLQVPRRELNRLADVVYGKGADPDFGKLERLDPRARRLWERLSWFLTTSATVQPQGPWLDYVAAGLPSGIQYTKSRAGYYLNYDANHGRKGRARLCLARLPGLRVLSLDLGHRYAAACAVWQTLTIEQMTNECRQAAHPAPSNDDLFIHLRHPTHKPQKSGRKKGRPVTKTTIYRRIGPDKLPDGTDHPAPWARLERQFLIKLQGEDRPARYASQKEIDEVNQFRNFVGLEPIVDRPRVDDLHSDAVRVARLGLRRLADAARIAFAMTAAKKPISGGHEVELTTAQRIEFLQDALLLWQSLAASRRYRDDWAEKLWQSWVVEKLGGPQPAEIADDLPRSQRAASLKTARQSLRKVAEKLSDGQSPSAAELHRLWAERWQQRQTEWRRHLRWLRRLILPRRKDHQQEDRPLQRVGGLSVKRIQTIRQLYQVLKAFRMRPEPSDLRKNIPAPGDRSLASFGRRILNHLERLREQRIKQLASRVVEAALGAGRISKPPGRDRRRPQQPVDRPCHAVVIENLQHYKPEDSRLRRENRQLMDWQARNLRKYIVEGCELHGLLFVEVSPAYTSRQDSRTGAPGLRCEDVSRTALQEAARRMHASHSRPSNSSPGGSQTQFEREVCRWINEFKRVEGSSSSLSARQAVLKAFLHHQASIPTSLSTILLPRRGGELFVSADPDSPLACGLQADLNAAANIGLKALTDPDWMGAWWFVLVDRASGQPVEEQVQGCPIWLSCGPLSNSNPATIDPSDSPTAARRSNGTGAKGRARANEYWWSSLSATTLPDHKAWQPTQDYWRDIEQRVVKRLLRLLDGSEWSED |
| 567 | Cas12 | MLDKFASLYPVTKTLRFRLLPQGRTEENMQVAKVLENDLERSEAAAVVKGLIKKYHLQFISDTLSGSTLSWQALTETLDKFKADHTATAELDSALAAYRCKLAELFTKSPKYKVMATPVSIIKEILKTETDPENIAALNKLNGYTYIIFDYVSTRMLTYSADAKATSLAYRLVDENYLRFYQDISAAAEISAVLEEAGFDNAEVEAFIRTDYNTCLTSEGIASFNAAAGSINQFVNVLLQQNPVLQSEPALRRHLQPLYKMLLDEAESKIIKFEDYGQLRDAVENFRRNFQDLPQSLIDIFAGRYDYSKIYVGYKYLNEASSQIAGGYNWKLLENALEDFYSKPYLVNGKLPVKYKTVVNKKMNQLAYSFTELQEALDAGDSGSSITDLFGKYAELHAAYAAADGNVFYKEYDRKSIASLKNYLDAVNAIARFIKIFAAPEVYVKDEGFYGIVDGAADKLRDFDLLYNMVRNYITKKPYKKSKVALTFNSSSFGRGWDENKIYDELTTIFTYNGKYYLGVINKNDKPDLAAAVSKDEGGYKRMVYKTFDIVKQLPRLSFTKAVKAHFAESDEDFIFDGPKFAKPLRVPKEIYLQSFTDNGDKLADSAKKYTKAYLDMSGDYKGYYEAIIKRIDYTKEFLSAYKSTSIYDLAFLKPAGKAAGSLCWTRHI |
| 568 | Cas12 | MCIRDRDLAVAALNRGDAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGQLNAIRNFLASDGYGAHDLVPVNYNGSNDRGAEVQVGNQKNWTVTSSAPVVVQYNNADTTLTVQGHTDRLIVTGSGNDTITLKDSGDDKVLLGDGNNTVVAGSGADTIVGGAGNDVLIGTNGGYGTELLGGAGNDVLRNAGSHGIYMDGGAGNDTFYGGTGPDTMEGGDGNDLMYANGRGSSIDGGAGNDTIYGGPGGDTLTGGDGNDLLRSDSAFGTKGSGNLLVGGAGNDTLWAGAGYDTLKAGSGSDTLISGTGSSQMIGGSSGNTTFEVAYHTGNDTITGSGSGNTVYLDGRDFSDATISNHSGVTTVSFSDGQVLKISGVQDIVFSDHDYKV |
| 569 | Cas12 | MSITRSIKVKLIVPRDASLEARQLREGLWATHLFVNDGCHYYERLLLEFRQRDVCVGKDDAGKDVIVPAAEWADRLRARLGRNGMVPSHIEAALPIFRELYENMVPSALKAKSGTGQAGRSWHSKLVSPTSRGGEASAARIDVLRPLLPVSGDDPAFEPAARALIEEAGDELLTSTGRCPAWVTAYRKGPEGSAWVEKLRIQLREAVEAGDFDPPSDPQILAAGAVPAAPPLGAGIDALRPLLPLLGGDPAFEPAARALVEDIGDELFTSTGRPPTWVTAHPTWVRAHRKDAECLEAADDFKWVERLRQRLRDDAKAGKFEQPLHERLGALGALPVAKPIGAGRVVSRADLTVFERGAMELAIEHLIGWESAGHRARAQYVERKKRHDDLLQWIEAEAPDALLAVRAYEAARTIHLATLGELGAAPQYTLRLREIRPWRKLREWLLQNPDATIDERRRRLATMQTNDPRGYGGEALAWLAAPERRALVEHPAGDVVTRIAVLNIRKSILDRSRLFPTCTLADPVEHPRFAKFGKPGDKNSAGYALAVDGVRREAIIKILVPRQDGLLVPTDLRVPFAPSGQMRDLRASGLDISYERQDGRGRQAAKLQGGNLMFDRTHFARCGAPGPEALGSVWIKVALDLSSPAASLAMKTATPVRTYLSTAVRGRPESTKYEKAAPPEGFRVLSVHMGLRTAATASMLRFGAPEEGGHEVPVSGLAGETLVAFHERTVTMKLPGEDPDTRTEANRGVAKRELRGLGRGIGCLKAIRRASASATPEDRAEALVIIETHVGQGDRHGWAPAEAVGRLDPHGDPDDWKTACAALYAAVEADLGVAISSWRKAARAGGATGMLGGKSLWAVDHLERSFRFLRSWDLRARPHDGDPRRPRPGYASKLLHHIDGVKDDRVKTTADRIVQAACGRAWIGGPTVKRGTQDVRLPGRWEQRGPRADLILLPDLTHFRFRSDRPRAENSRLMRWAHRQLAIYVRMQAEVEGILVADTGAAFTTRFDAWTGAPGVRCEPVTADHLRGIAKREDYWLARLLREGALKHLRIDPASLRVDDLVPMDHGKILVALDGVDLPGLRILDTDVNASQGLGRRYIEGHGLAYRLPGARVPRGEGEREAAVVHIKGKRLASAMGGTVVVLRASEGPGDITWTAEVYDRPQGARKALGLSLAAFNSIATAAVDDEGPAPENDDEALEEEAEEALGIATGERIVFFRDPSGAVAGGGWLEASAFWGIANRMVTDRLRELGRLG |
| 570 | Cas12 | MAQASSTPAVSPRPRPRYREERTLVRKLLPRPGQSKQEFRENVKKLRKAFLQFNADVSGVCQWAIQFRPRYGKPAEPTETFWKFFLEPETSLPPNDSRSPEFRRLQAFEAAAGINGAAALDDPAFTNELRDSILAVASRPKTKEAQRLFSRLKDYQPAHRMILAKVAAEWIESRYRRAHQNWERNYEEWKKEKQEWEQNHPELTPEIREAFNQIFQQLEVKEKRVRICPAARLLQNKDNCQYAGKNKHSVLCNQFNEFKKNHLQGKAIKFFYKDAEKYLRCGLQSLKPNVQGPFREDWNKYLRYMNLKEETLRGKNGGRLPHCKNLGQECEFNPHTALCKQYQQQLSSRPDLVQHDELYRKWRREYWREPRKPVFRYPSVKRHSIAKIFGENYFQADFKNSVVGLRLDSMPAGQYLEFAFAPWPRNYRPQPGETEISSVHLHFVGTRPRIGFRFRVPHKRSRFDCTQEELDELRSRTFPRKAQDQKFLEAARKRLLETFPGNAEQELRLLAVDLGTDSARAAFFIGKTFQQAFPLKIVKIEKLYEQWPNQKQAGDRRDASSKQPRPGLSRDHVGRHLQKMRAQASEIAQKRQELTGTPAPETTTDQAAKKATLQPFDLRGLTVHTARMIRDWARLNARQIIQLAEENQVDLIVLESLRGFRPPGYENLDQEKKRRVAFFAHGRIRRKVTEKAVERGMRVVTVPYLASSKVCAECRKKQKDNKQWEKNKKRGLFKCEGCGSQAQVDENAARVLGRVFWGEIELPTAIP |
| 571 | Cas12 | MNIIMENFEKFVNLYELSKTLRFELIPFSQTKVELEKDWIIEKDREIEEKYHIIKEKLDTLHIKFVWQALEWVDLSLLEEYAELYFACKKDTKNKKLKSKFEKLEKKIRQEITSFFDAEWNKWKEKYGFLKKWWTSFLTEKEILDVLIDIFPENKDDFEIFKWFFTYFSNFNESRKNFYKDEWKAGQIATRAIDENLTTFLENIIKYKNFKKENPDFFTENEEKVFELDFYNFCLTQKWIDNYNEIIWAKSLEEWKNTQGVNQRINLLKQKNEKSNKKNLSYPKFDILYKQILSEKSENDFIPNIENTEELFTVIQKSIKENDKKITEIDKLFKKFFLEENNIDIWKVYISKQAVNTISSKYFENWSSLWWYLWENSKKKYFSLWEIKEALEDIKEKNIFKGEYYNNKIAFENKSNFENFLAIFYYEFQTNLSLLNWNQNNLESLQEKEFKKEEKQVDIIKKYFDSVMDLYAMSKYFFVDLKQAKNFPKDIEFYNDFDLYFSDYEPWKVYNLVRNFLTKKEVKTDKFKLNFSNSQFLTGWDKDKEKERFWVILRKNEKYFLAILKKNNNKIFENYRENNPTDFYEKMEYKQLNNVYRQIPRLGFPLQKKLDSLKWKELEEYLEKYKNNFWYNKEIAFIKEEFDIFQKNKEKWEKFDREKLKKLIDYYKKVVLEKYSDLYDLKKLENTDYDELVNFYDDVEKSMYSLHFTKIETEFLENLEKNWEIYLFQIYNKDFSDYKKENTKENIHTKYFKHLFSEENLENLKIKLSGWAEIFFRDKTHNLKQKLDKNWKKMFYWENKDKKVLEHRRYAKDSYGFHISITLWANNWDMYKFNQFFNKNFTPKHIIGIDRWEKHLAYYSVIDLEGNLVETDTLNIVNGINYLEKLENIEKSRMQERKSWWEIENIKNLKDGYISAVVSKLTELIEKYQAIIVFEDLNLGFKRWREKIERQVYQKLELALAKKLNYLTFKNKKDCEIWGVLNGIQLVPRVKDYQDIANYKQSGIIFYTNPAYTSTTCPECGWRKTLKFPSKITKTSILEFFKEIQMSFYWEKFSFTYENILWKSETLFSNVKRTQWNNKSRKIEHKENITQELKSVFEKYNINLWENISEKLQQTDMLLEDLKVLFYNFKLINNIRNSDSKLEEDIISCPCCLFNSENWFKGAYFNGDANGAYNTARKGIIMLENIKVNPERSNLFVRNEEWDEFLREEF |
| 572 | Cas12 | MSKLFENMTNLYSLNKTLRFELKPVGNTRELIESKDFFKNDEEKAENYQFMKEKMDKIHRNYIQKSLETIKMLPILDQTEQKRLKKEDIKNELKSLRSFISAAFVSVKDLLSNKIIEWLISEASIEEKEKIKKFDKFFGYFKTYVQNRGNLYKAEDKAGQIAFRLIDENLPRFFKAKQIIEEIIKKTPDFVVKERNEKQEETEKKITEYLSIFYLNSYCHYLSQSGIDLFNEIVGHINLSINLYKQKTGVKFSLIPLLYKLPLAPRKQISRLPKQIENPEELELIVKSVLDRIDQKINPFINNILQTVLEENSVYDLNWIYISIKTTEQWWSIGLSGRNSLRELGYNQSKNWEEPVLKAEKNKFPFISLGEIKSFLDAKQLAWDDIKSVFWEGKSQSLMNWSWSNIFFHLIGKEISSLIEKYLHSRKNYQISQSKENQKHVLDNSLSLWRLLGWFVLMHKKTHLTPEIKESFFYDWENGLDAIVFDENSDPIHTIYDKVRNCLTKKPYSNKDKIKVNFDCPYLLWGWDQNYDAFGWLIFHDWKKYFLWLIKGSWLNMEEKNKLYEWINPMNSITKIIYDYQKPDFKNVPRLFIRSKWDTFAPMVREYWLPVNDILYLYDNELYKPDKKNPWKHKSYLRRLIDYFKLWLSKHKSFKHYIFKRKESDQYENLAEFYSDVEFSCYAIKKEKVNFDQVKALCESERLYLFEIYNKDYNQFSKWKNKNLHTQYFEALFEERDNNLFMLSGGWSIFWRESNWKIVEKIRSYSPKYNIEIIDKRRYTKNKLMIHIPIVLNFCRNQEWRVNDMIKSLIQSQSNNFTILGIDRWEKHLLYYSLIRQDWTIIKTGSRNTITNKIKIVDYHKKLDDREKKRDEAQANWEQQEQIKDLKKWYISQVINEISKMIIEHNAIIVLEDLNGWFKRWRQKVEKSIYQQFELALAKKLNHLVFKNRWDTESWGTMKAYQLTPLVAQFQDLSFQTWVVFYTPAWYTSTTCPCCGWRKNIYFKYENEKQAKIELEKLNIVRENNYFSITYTAEWWNKKWKITWSVLNKTDRILTTKWQTRLQYDRASKNTKEYDITTDFNSVFTAKFLDYKIRLENAGSKQCRNLINSINLLLKIRNAKSGTDIDTIQCPACEFHSQWWFQWNEFNWDANWAYNIARKGKVIIDKIVKGEKNTTVSQIEFDNEIQK |
| 573 | Cas12 | PAYRHPDPLLHPVFCDFGCSRWQICFDVRKNVRTTSPRRLCLTLFTGSGMELVPFSWQSKRLARDLALEQRHRNPEASEVTRADRLGRAAAGVSAGGAVSIKNIFNEENWNGRLQAPRSQLAAIAARVDKHGWDAKALRMLDRLRWLITFSPRLEPTGPWIEYALRIPDDAAAKPFLSRKNGFAVLHRSNDDRKGLAKLILSRLPGLRVLSVDLGHRYAAACAVWETLSADQVREACQAAGHDGPTECDLYLHLKKDGRTVIYRRIGADTLSDGTPHPAPWARLDRQFLIKLQGEEHKAREASNAEIWAVHQMEAALGRSVPLIDRLVASGWGQRTEGQRARLEALKQLGWRPVAEKLDSDDEPGVMEESPAIKPSLSIDELMSSAVRTMRLALKRHGDRARIAHYLITDEKTKPGNVKEQLDENGRIELLQDALVLWHDLFSSKGWQDNKAKELWYVHIATLPEYKAFQSSGEGHAGPERRGKPEEDREKLGVVAQALAPNVTLREALHNAWKKRWEEDDARWKALLRWCKDWILPRGEAANSPAIRKVGGLSLIRLATLTEFRRKVQVGFFARLRPDGKKAETGEKFGRKTLDALEELREQRVKQLASRIAEAALGIGKEHVFPTAKEPQKKCWCGSVHRKKDPKRPRERVHSPCHAVVIENLTHYRPEETRTRRENRQLMSWSSGKVKKYLAEACQLNGLHLREVNAAYTSRQDSRTGAPGIRCQDVPVKEFMRSPFWREQVAQAKKKQAEGKGDARERFLCDLDEQWKNRSEDEWKKAGPVRVPLRGGEIFVSADGTSPTAKGLHADLNAAANIGLCALTDPDWPGRWWYVPCDPASFKPIMDKVEGSAAVQPDQALRQPAQAQRGDAARDRKKRGKVGGRSREVVNLWRDVSSRPISPNDSWQEFTPYWNDVQARVVNILRQSAGLTRGQGEP |
| 574 | Cas12 | MPLRSINIKMRLKRAAEGRALRQSLWLTHSVVNCAVAEIERVLLLCRGRGYWTGDDEPVSAETVQQQALAFARETQARNGQPGIGGDAEILAALRGLYEAIVPSVNRDEQGRPLEGNAQAAGGFAGPMMDAESEGFQSVFDKILEPLPSWVAKMTGGTRGWEQESVQWLKSPESERLQHASGSPPAWVRRLRTGEPWQEAFVQDQENKRKEVKGVPSLIQRLKKELGLLPLMRPPITLQFEDKRSGLTPWDRLTLRLAVAHLLSWESWNHRAADEHGRVTERLARLETEAAPLASLIEGLREYEKIRHEELKRVAQANDENPFRIGARGVRGWDRVREAWLGATDDTKEGRFTSVAALQTKLGGRFGDPDLFRWLAEEGREHLWGEHDPLPILARLNALSRLLRRKKDHAIYTAPDARLHPRWTAYEAPGGGNLRNYSFEISGNDLALRLPLLRRVESGLEEDSQRAEIALAPSGQFQSASWKGDGKPNRHLTYYSAHEQFSAELGGAEILYRRRHLENRKVKELEQGDIGPVWLKLVLDVQPNAPEGWFTPRGRVVTPPTVHHFNTALVNRSKHAPDLVPGLRVLAVDLGVRTFAACSVFELVQGEPGNGMAFLADQERDLWARHERSFLLPLPGEAVDSQLLAARRAAYNQLGLLRRDLGRLKGILRLSVKETAENRCGSLEELLASLEDEWNRGNAPAIDVAALLSAKGCLEMPQPAWEAAIAGTYQAAERELGNRVRDWRRTTRPRTTGEDDRRQRRGYSGGKSAWAVEYLDQVRRLLQGWSLHGRSYAQIRRLDREKMGTFAAGLLDHINALKQDRVKTGSDLIVQAARGYLPTGKKGWIKQYEPCRLILFEDLARYRFRTDRPRRENARLMRWNHRQILTETELQAEIFGLLIGTTGAGFSSRFHARSGAPGCRTRLLTAEDLRSPTLAKQLELLAETQGIDPKCFRPGMQIPWDSGADFVTLDSEGKLVQIHADVNAAQNLQRRFWTRFRDAYRISAVEIKQDGRTSWYPDREGVRLRGALSTIVGGDGYARLVAADDDDGFVLEKVTRPHWRRAAGAQAESGDDVGLDEVALEMAEAMDTDLERGEGRLVFFRDPSGSVIRADRWYQAKAFWGQVRSRVTRALGLRRPGASS |
| 575 | Cas12 | MLDKFASLYPVTKTLRFRLLPQGRTEENMQVAKVLENDLERSEAAAVVKGLIKKYHLQFISDTLSGSTLSWQALTETLDKFKADHTATAELDSALAAYRCKLAELFTKSPKYKVMATPVSIIKEILKTETDPENIAALNKLNGYTYIIFDYVSTRMLTYSADAKATSLAYRLVDENYLRFYQDISAAAEISAVLEEAGFDNAEVEAFIRTDYNTCLTSEGIASFNAAAGSINQFVNVLLQQNPVLQSEPALRRHLQPLYKMLLDEAESKIIKFEDYGQLRDAVENFRRNFQDLPQSLIDIFAGRYDYSKIYVGYKYLNEASSQIAGGYNWKLLENALEDFYSKPYLVNGKLPVKYKTVVNKKMNQLAYSFTELQEALDAGDSGSSITDLFGKYAELHAAYAAADGNVFYKEYDRKSIASLKNYLDAVNAIARFIKIFAAPEVYVKDEGFYGIVDGAADKLRDFDLLYNMVRNYITKKPYKKSKVALTFNSSSFGRGWDENKIYDELTTIFTYNGKYYLGVINKNDKPDLAAAVSKDEGGYKRMVYKTFDIVKQLPRLSFTKAVKAHFAESDEDFIFDGPKFAKPLRVPKEIYLQSFTDNGDKLADSAKKYTKAYLDMSGDYKGYYEAIIKRIDYTKEFLSAYKSTSIYDLAFLKPAGKAAGSLCWTRHI |
| 576 | Cas12 | MSRSIFSPFTNLYPIQKTLRRELKPLNENFQHDPALSALRNSEIPQRDEQREKDYQAIKPLLDEFHNQFITESLHSLEPQDRSDFILFYQTYQKKKKNKAEISEKELKSLDEEFESRTKALRNAIGTSFSVTAELRKSNPDYVSEKGKPFLTQKSYKILTEAGVLWLLEKKYTSDPEKLALIRRFGNFFTYFTGFNQNRENYYATDEKSTAVAYRAINENLLTFANNCELFEKLSVLSLSELEKKTFNPDSYSEYLTQSGIVFYNEMLANIRSKANLYTQEHKAKLPQPKLLYKQIWSPRGDTIPFDLIASEAEFQETLHTMIRETDQRIPEFNKLLEQIFEEKVDLSQIFFSKTSLNIISNRYFSSWHTLLEKGVELKLFKFKKNDEESFKLPAYLSLAELKELLESAPFQMAEKADADEEKHHQASLFKLQRENLHLEKSHSNWELLLKSMKSDFESFWTWEGEFWSYTLAKKALQSLSALESTNQEHKNLIKMLLDNALYAYRMLKWFKVDTSKLGFVPEGEFYPSLDQLLQDYPLPKWYDMIRNYLTRKTYSQAKLKLNFDCSTLLNGRDKNKEIQNLSVILRKDGKFYLAIMKKDQNKFFENSALYEGNLGTMEKMDYKLLPWANKMLPKCLMPGSDKKKYGASDQVLELYAKGSFKKSEKSFNLADLHTLIDFYKLALPKYEDWKVFNFQFQATENYQDISQFYREVEQQGYLLNWRKVNEKLIKQGIKDWSLFLFQISSKDFEGKSKTPDLQTLYWQQLFEFSTNVKLNGEAEIFFRPWSMKKEKKKLKVDNYDVFKHKRYTEDKILFHVPITLWFGNNEVSPSAPSKFNQKLNQELIIPHFDDLHVIGVDRWEKHLAFYSVVSVKTGKIVKQGTLNLLNGTDYEAKLSQKAENRLHARQNRDTIEKIADLKNGYISQVVNKLVELVLEYNAVIVFEDLNAGFKRGRQKIEQSVYQKLELALAKKLNFIVKKEKAVGEPWSVTSAYQLAPQINTFWDIKWKQRGIMLYTRANYTSVTDPLTGWRKQYYFKKGSSEEMKAQFFKSFKNLTRDAGQEAYIFDDGTWLLYSNVERRRGKRGDHRERTQIKYDPSLELDTLFSKYQIEKSDSLFDQLKNRELPQTFWTSFFRIIDLIMQIRNTDDEGRDIILSPIGNPQERFDSRKRYNQLPRDEKGGIIEESAFEYPTSWDANGAYNIARKGVMMLERIKENPEKPDLLIRDAEWDKKITRK |
| 577 | Cas12 | MKNFQDFTNLYELSKTLRFELKPIWGTKKLIEEKNILKLDKKKRENYEKVKPYFNKIHQEFINFALRNPNFDFSQFEEKYLNWLKDKKNKDLLKEKESIDKIFLEKIWKLFENSVKDFLKENGFESIVKSEDQNLKFFRRKEIFEVLQEKYGSELETQMVNKDWEIKSIFNGWEKWLWYFDKFFNTRDNFYKTDWTSTAIATRIIKDNLKIFLENTIIFEKVKNKKIDFSEVEKNFSVSIDTFFEINNFNNCFLQDWIDFYNKVIWGETLENWEKLKWLNEIINKYRQDTGEKIPYFKKLQKQILSEKDWVFIDKIEDDGGFYEVLKNFYKNAAEKEWFLKNIFENFYTISDKNLEKIYFNKIAFNTISHKFWSALEFERILYEEMKKEKADWIKFEKKENKYKFPDFIQIIFIKRSLENYDSENLFWKERYYKSEENVDWFLEKNNNNIWEQFCKILNFEFLNILKRRIIDEAWEEYEVWFEISKNILWEKLENFELNQENKWIIKDFADYSLALYSFWKYFAVEKWRNWDLNIDISDDFYGWEDWYIEKFYNTGYDEIVKPYNLMRNYISKKPWEDSKKWKINFETSSLLSWWDKNLESNWSYIFQKWNKYYIWIINWSKPAKEVLEKLYSWNGEKIKRFIYDFQKPDNKNTPRMFIRSKKDSFSPAVGKYNLPVEDILEIYDNWLFKTENKDNSNYKESLSKLIDYFKLGFSKHESFKHFNFVWKDSKEYENIADFYRDVEKSCYQITSEFLDFEELKKLTFKKHLYLFQIYNKDFELDESLQKNWYNFRDEWQKNIHTKYFEALFLEENILRKSWAVFKLSWGWEVFFRKESIKAEKEKRKNIEVTKNRRYTEEKYFLHFPIQVNFKNEISWNFNQEINKFLANNPDINVIWIDRWEKHLAYFSVINQKWEILESWSFNKIENYNKNWEKLLFPEREIKEIHKDWSLIDLELVETWRKVDYVDYKLLLEYKERKRLLQRQSWKEVEQIKDLKKWYISALVRKIADLIIKHNAIVIFEDLNFRFKQIRGWIEKSIYQQLEKALIDKLNFLVNKNEINLEKAGSILKAYQLTVPVDSLKEIWKQTWVIFYTEAAYTSKIDPIKWWRPNLYLKKQNAEINKENILKFDNIIFNSKENRFEFTYDLKKFFWKDSKFPAKTVNTVCSCVERFKWNRNLNNNKWGYIHYENLTDWKLANKEQKEDEFSNFKELFEKYFIDINWNILEQIKNLDTKNNEKFFSSFIDLFTLVCQIRNTNQNAKWDENDFILSPVEPFFDSRKSQNFWKSLPKNWDENWAFNIARKGLIILNRISENPEKPDLLIFNADWDNFARNI |
| 578 | Cas12 | RPGASAALSLTPLKTLFSENFSSFMCGNWQIINDSLKTYYNENIKSKGKAKEEKVKKAIKAIEYKSLADINQLVERYNNDELNRKAEEYISAINEKIKDLDVNEIEYDEKINLIENETKSEEIKSKLDSIMEIMHWTKMFIIEEEIEKDVNFYNEIEEIYDELQPLVTIYNRIRNYVTQKPYSEEKIKLNFGIPTLANGWSKTKEYDNNAIIMIRDGKYYLGIFNAKNKPDKKIMEGHQSEENGDYKKMIYRLLPGPNKMLPKVFMSKTGIAEYKPSQYILECYEQNKHIKSDKNFDIKFCRDLIDFFKTSINRHPEWSKFNFKFSETSEYEDISTFYREVEKQGYKIEWTYISEKEIKELDENGQLYLFQIYNKDFSEKSKGKENLHTMYLKNLFSEENLKNIVLKLNGEAEVFFRKSSIKKPIIHKKGSVLVNKTYNENGERKSIPEEQYTEIYKYLNSIGTNELSEKSKKLMEEGKVEYYKANYDIVKDYRYSVDKFFIHLPMTINFKAAGFSPINNIALKNIALKDDMHIIGIDRGERNLIYVSVIDTKGNIVEQRNFNIVNGIDYKEKLKQKELDRDNARKNWKEIGKIKDLKEGYLSLVVHEIAKLVVKYNAIITMEDLNQGFKRGRFKVERQVYQKFETMLINKLNYLVDKDLAVDQEGGLLRGYQLTYIPESLKVLGRQCGYIFYVPAAYTSKIDPTTGFVAIFNYKGMTDKDFVTSFDSIKYDDERGLFAFEFDYENFVTHKVEMARNKWTVYTYGERIKRKFKNGSWDTAEKVDLTYQMRSILEKYEIEYNKGQDILEQIEELDEKAQNGICKEIKYLVKDIVQMRNSLPDNAAEDYDAIISPVINNNGEFFDSTRGDEDKPLDADANGAYCIALKGLYEVMQIKKNWNEETEFPRKELKIRHQDWLDFIQNKRYL |
| 579 | Cas12 | MQCGSLRHNSKSLDSALAYPARLTTGGNEGKTSEAHIENDSLPEITRRPSRLARDFAPSNPFPFMKRIYQGQITGMQFHGAAGEQAVPANQNWEQALWDHHGLFHDAVNYYLVCLLALARPGNPVYAIREKLDAHNGAEPDELMVWRTFRRRGCNRPGLRDSVAKYLTPGNDEPTTEECFAAVLAGNPLGATEEGRAILNEGLAQLLGKCTGESGCRNSAKEYLPRFTKPDFKGNFEEDTARLNRAEASKRLPFYLHDPATTPNSPALDEFGVLSIALPNPKRAELTGAEARDKLLEFSREWSARLPDGSADWRRLEAKITALGDSLAIPGYTSGSAKGENRFQLYAMFLFCFVEKSDFTLGLLRQTTRRPVEGEEPPPRSNHSATAGDPIRIARGGRGYVFRAFTSLKQWGGDTGGDLKWPKFDMAAFCEALKALHQVEAKAKQRAEERSKKQALLDYQRGRIRRFKPTANSEDATPPPVLAGDPRISRLEQLLNTDLQDEYEMSEGVAVAYGLHPRTIRGFREIRKRWISAVGNAPFSEAARATLIACVRSFQSENPGTVGSARLFEALAEESNWIIWREPSAAEQQSWRENADLPESAEFALDPLQALTDERELKDEIERLSGPIRFTPADAEHSRRQFYFSDVSQIDKRNRFRPRLNEVEVELAVRPNGHWTNVWVTLQYSAPRLLRDQLPTADEAGGGWQQAMMAALNLRAPLKKGGEAVSFADCAALSLMPDISPDGEKRLLLNFPVELDGEAIANQLGRSKRWETLQFGGADDESYWLRWPKTWIDETKVRRKAAPPKWWLSKEPFSVLGVDLGQRDAAACALVQVSPGNCPTGVCRHVGSADGVDWWATVRSMNMLRLPGENAKVMRDGRFQEELSGSRGRSASIDELKEAGDICARLGFVADTILGANGRALSFPELNDRLLFSLRSAQSRLARLQSWSCVAHADVPPARREGILRDISEAKDDPLGLKPLATAGNLEAIASTLREVILQERTSISAQLVRVADRILPLRGRRWEWVARPESPSNHMLRATAPDTDPRRKLVAGQRGLSLARVEQLESLRQRCQSLNRALMQVPGTPSKLGRRSRGVELPDPCPDLLDRLDALKEQRVNQTAHLILAQALGVRLRIHQVSGPQRTRSDSHGEYERIPGREPVDFLVLENLDRYLASQGRSRSENSRLMKWCHRAILLKLKQLCEPYGLRVLETPAAYSSKFSSRDGTAGFRAVEVTPDDLGSHRWRKHSERLADPGASLSRDEREESTRLMAFAERLKALNQDLIARQEAARSSNQPFRPKWRTLLAPQQLGPIFVPAVGKPLQADINAAINIALRAIASPDVDDIHLRIRAARSGDKFVVRAENTRERARWGSAEAEIGLPTGAGAKEIEERRSLLTEARVNFFFDPASVAAFDHGKVRDAKLAVTSGRGLWGTLRREEWAIVGKINNDRLAAHGLGRPFAERVDL |
| 580 | Cas12 | MSQRDPLPSPTPRAYTLRLSTPDADNGAWRERLWKTHEVVNSGAEAFGDWLLSLRGGLDHRLVAVKVRVGRGASKTEREPTEDERRERRIVLALCWLSVESKKGAPQGRVVEDPVDALKRILGQRGLSEADAEQWVFDCRDSLSATIRDDATWVDRSAAFDETVQRLGSSLTREHAEQVIIEFFGDIHDYLALPKNMEEDGVTSPRGGSGKEFRTQARSWLSENWGAGQKGDKAQIVDALRKCSRCIAQEKPSTGSDLLRVLVRSLGGSPEETLRFDELRKLVGWRTGRRSTGAFALQRTVDMDRLSESDLESLEAKLTVEIDAKSPEASRSVPQWVESMRTAVEREVGMPFRSTRDLIGEFGVMLDHAARRVSATHSWIKRAEAERRKFEGDAAKLAQIPEEARQWLDQFCEDRSQELNALEPYRIRRRAVEGWESVVRTWAKADCKAEGDRVKAVRALQPEIEKFGDAALFEALAAEDALCVWLLDGKATPRPLLDYSAATDAQYRKRRFKVPAYRHPDALLHPVFCDFGESRWTIEFSAHRALGRRRKAQQLVDNKTVALQKAQERLRKAKAESSRSRAEEKVRAAEHALEAARKKLAFLQYRRALAVCLWTGKGVESTSLRWHSKRFAKDFAIGSSPEDGASTGVPVARADRLGRASANVPRGASVTISGVFEQKEWNGRLQAPRDALNAIAKVRDDETLPAADRSRRVQQMLSRLPWFVTFSARLVPQGPWLDYASEHSALGLRVDPKYWPHAEENRQRKGMARLILSRLPSLRVLSVDLGHRYAAACAVWETLSAQEMQRICREMGSAPPSPDDLYLHLRRADGNGKQRTTVYRRIGPDTLPDGGQHPAPWARLDRQFLIKLQGEDESARKASDSEIAVVKKFEADLGRPAMQRRSLRVDDLMSAAVRTARLALRRHGDRARIAFNLIADRRFRPGGAEEPLTDETRVDLLADTLATWHGLFSGGQCHDERAEEQWNEHIAPLLASSGASLSVPSDGDAVAATPARRRRKEVRGKLVPAAMELARRDLSQLSVLWVARWHQDDESWRARLRWLRDWILPRGAKADSGAIRHVGGLSVTRLATIRSLWQLQKAYRTQPEPEDPRKNVPKKGDTSLDDFGRTILDDLEHLRENRVKQLASRICEAALGVGVEQPNGGAKDPKRPQERRFSPCQAVVIENLTRYRPEETRTRRENRQLLNWSAGKVKQYLSEACELHGLHLREVSAAYTSRQDSRTGAPGIRCQDIPVVDFVRENGPCWGRLRSAQQSSGGTAEDQLLLALYERWSEKERTWRDQSGNVWAMDGEGRWVARNGTQLPQGSGLTPHPIRVPQRGGDIFVSAAPRSPAANGIQADLNAAANIGLWALLDPDWEGRWWRLPCSAMDLKPVKGSVNGSAVIHLDAPLKAAATKGAAKKDVVNLWRDISVRSLTEGVWRGYDAYWNLARYRVVQTLRPQVGIGSDE |
| 581 | Cas12 | MFKGDAFTGLYEVQKTLRFELVPIGLTQSYLENDWVIQKDKEVEENYGKIKAYFDLIHKEFVRQSLENAWLCQLDDFYEKYIELHNSLETRKDKNLAKQFEKVMKSLKKEFVSFFDAKWNEWKQKFSFLKKWWIDVLNEKEVLDLMAEFYPDEKELFDKFDKFFTYFSNFKESRKNFYADDGRAWAIATRAIDENLITFIKNIEDFKKLNSSFREFVNDNFSEEDKQIFEIDFYNNCLLQPWIDKYNKIVWWYSLENWEKVQWLNEKINNFKQNQNKSNSKDLKFPRMKLLYKQILGDKEKKVYIDEIRDDKNLIDLIDNSKRRNQIKIDNANDIINDFINNNAKFELDKIYLTRQSINTISSKYFSSWDYIRWYFWTGELQEFVSFYDLKETFWKIEYETLENIFKDCYVKGINTESQNNIVFETQGIYENFLNIFKFEFNQNISQISLLEWELDKIQNEDIKKNEKQVEVIKNYFDSVMSVYKMTKYFSLEKWKKRVELDTDNNFYNDFNEYLEGFEIWKDYNLVRNYITKKQVNTDKIKLNFDNSQFLTWWDKDKENERLGIILRREWKYYLWILKKWNTLNFGDYLQKEWEIFYEKMNYKQLNNVYRQLPRLLFPLTKKLNELKWDELKKYLSKYIQNFWYNEEIAQIKIEFDIFQESKEKWEKFDIDKLRKLIEYYKKWVLALYSDLYDLEFIKYKNYDDLSIFYSDVEKKMYNLNFTKIDKSLIDGKVKSWELYLFQIYNKDFSESKKEWSTENIHTKYFKLLFNEKNLQNLVVKLSWWADIFFRDKTENLKFKKDKNGQEILDHRRFSQDKIMFHISITLNANCWDKYWFNQYVNEYMNKERDIKIIWIDRWEKHLAYYCVIDKSWKIFNNEIWTLNELNWVNYLEKLEKIESSRKDSRISWWEIENIKELKNGYISQVINKLTELIVKYNAIIVFEDLNIWFKRWRQKIEKQIYQKLELALAKKLNYLTQKDKKDDEILWNLKALQLVPKVNDYQDIWNYKQSWIMFYVRANYTSVTCPNCWLRKNLYISNSATKENQKKSLNSIAIKYNDWKFSFSYEIDDKSWKQKQSLNKKKFIVYSDIERFVYSPLEKLTKVIDVNKKLLELFRDFNLSLDINKQIQEKDLDSVFFKSLTHLFNLILQLRNSDSKDNKDYISCPSCYYHSNNWLQWFEFNWDANWAYNIARKGIILLDRIRKNQEKPDLYVSDIDWDNFVQSNQFPNTIIPIQNIEKQVPLNIKI |
| 582 | Cas12 | MLSFNWVLFFFFSKMSRSIFSPFTNLYPIQKTLRRELKPLNENFQHDPALSALRNSEIPQRDEQREKDYQAIKPLLDEFHNQFITESLHSLEPQDRSDFILFYQTYQKKKKNKAEISEKELKSLDEEFESRTKALRNAIGTSFSVTAELRKSNPDYVSEKGKPFLTQKSYKILTEAGVLWLLEKKYTSDPEKLALIRRFGNFFTYFTGFNQNRENYYATDEKSTAVAYRAINENLLTFANNCELFEKLSVLSLSELEKKTFNPDSYSEYLTQSGIVFYNEMLANIRSKANLYTQEHKAKLPQPKLLYKQIWSPRGDTIPFDLIASEAEFQETLHTMIRETDQRIPEFNKLLEQIFEEKVDLSQIFFSKTSLNIISNRYFSSWHTLLEKGVELKLFKFKKNDEESFKLPAYLSLAELKELLESAPFQMAEKADADEEKHHQASLFKLQRENLHLEKSHSNWELLLKSMKSDFESFWTWEGEFWSYTLAKKALQSLSALESTNQEHKNLIKMLLDNALYAYRMLKWFKVDTSKLGFVPEGEFYPSLDQLLQDYPLPKWYDMIRNYLTRKTYSQAKLKLNFDCSTLLNGRDKNKEIQNLSVILRKDGKFYLAIMKKDQNKFFENSALYEGNLGTMEKMDYKLLPWANKMLPKCLMPGSDKKKYGASDQVLELYAKGSFKKSEKSFNLADLHTLIDFYKLALPKYEDWKVFNFQFQATENYQDISQFYREVEQQGYLLNWRKVNEKLIKQGIKDWSLFLFQISSKDFEGKSKTPDLQTLYWQQLFEFSTNVKLNGEAEIFFRPWSMKKEKKKLKVDNYDVFKHKRYTEDKILFHVPITLWFGNNEVSPSAPSKFNQKLNQELIIPHFDDLHVIGVDRWEKHLAFYSVVSVKTGKIVKQGTLNLLNGTDYEAKLSQKAENRLYARQNRDTIEKIADLKNGYISQVVNKLVELVLEYNAVIVFEDLNAGFKRGRQKIEQSVYQKLELALAKKLNFIVKKEKAVGEPWSVTSAYQLAPQINTFWDIKWKQRGIMLYTRANYTSVTDPLTGWRKQYYFKKGSSEEMKAQFFKSFKNLTRDAGQEAYIFDDGTWLLYSNVERRRGKRGDHRERTQIKYDPSLELDTLFSKYQIEKSDSLFDQLKNRELPQTFWTSFFRIIDLIMQIRNTDDEGRDIILSPIGNPQERFDSRKRYNQLPRDEKGGIIEESAFEYPTSWDANGAYNIARKGVMMLERIKENPEKPDLLIRDAEWDKKITRK |
| 583 | Cas12 | MLDKFASLYPVTKTLRFRLLPQGRTEENMQVAKVLENDLERSEAAAVVKGLIKKYHLQFISDTLSGSTLSWQALTETLDKFKADHTATAELDSALAAYRCKLAELFTKSPKYKVMATPVSIIKEILKTETDPENIAALNKLNGYTYIIFDYVSTRMLTYSADAKATSLAYRLVDENYLRFYQDISAAAEISAVLEEAGFDNAEVEAFIRTDYNTCLTSEGIASFNAAAGSINQFVNVLLQQNPVLQSEPALRRHLQPLYKMLLDEAESKIIKFEDYGQLRDAVENFRRNFQDLPQSLIDIFAGRYDYSKIYVGYKYLNEASSQIAGGYNWKLLENALEDFYSKPYLVNGKLPVKYKTVVNKKMNQLAYSFTELQEALDAGDSGSSITDLFGKYAELHAAYAAADGNVFYKEYDRKSIASLKNYLDAVNAIARFIKIFAAPEVYVKDEGFYGIVDGAADKLRDFDLLYNMVRNYITKKPYKKSKVALTFNSSSFGRGWDENKIYDELTTIFTYNGKYYLGVINKNDKPDLAAAVSKDEGGYKRMVYKTFDIVKQLPRLSFTKAVKAHFAESDEDFIFDGPKFAKPLRVPKEIYLQSFTDNGDKLADSAKKYTKAYLDMSGDYKGYYEAIIKRIDYTKEFLSAYKSTSIYDLAFLKPAGKAAGSLCWTRHI |
| 584 | Cas12 | MNRIYQGRVTKVEILNGKNADGQPQELPNWQTALWQHHELFQDAVNYYLFCLAALASSASSPMGKLRNQLGSVWEPFGRTGRRFKGLRDSVGPYLLPDRPTPSIEEAFAAALAGNNSQPELLQLAVDALVEDLGGDAAIQQEGRGYLPRLCSPAYNGQFPRGANSLQKEAAKIRLPTLLHNVATSRNLDALAAELEFGFFANPVPNAGPIAGQEARNKLLEALAWLKAKDTRLTHSANELEKRIGVLPDSVSFPTYSGGSINKEALKHRFFAYLLFQHVERSLVTFEILRDSYPTPKPSARKSSKATPDTMAQLTQFGDDPVKLARGTRGYVFRAFTSLPCWGAKAPSDILWSEFDIAAFKEALKTANQFRLKTKERLDKADELAGELAWMNGEKSKFKPSEHSEQEPPAVLKGDPRFEVLKQLFEVELAEEHYLAEGESVIYGLHPRTLRCYRELVERWNKTVQPGEVFTEATSKKLVASTDKFQAENKERIGSVTLFKKLLERDYWCLWQTPDVQTVAARQKAGFSSNIIEDYQRYLELQTDIARLKEPIRFTPADAEQSRRLFMFSDLAGKSKHKHLPNATPYGFAVDVALAASEGGVWKETRVRLHYSAPRLRRDGLRKGVGEDLKRTAWLQPMVAALNLSEPAPQDFSKCAVSLMPDKSWKGLRHLLNFPVTLDPTVVQKAIGSQARWANQFVAFGKGATEQKFFLRWPEEVSASQKKAGPWWDALRAFSCLSVDLGQRDAGAFALLDVRANADWGKKPSRFIGEADGRNWRAALAAAGLLRLPGEDALVWRDGKWQEELYGEKGRLATKEEWLETNAIFSALEQNSEEWIGADPKRRSFPELNSKLLVVARRGQSWLARLHRWLWMLGDENKRERALRELLEQERQEAWRKRAEGKDLLALKQSLTAEIQRLNGLLPEQLVFLANRCLPLRNRKWAWNQHPDKAFAEKGCHLLEMVEAPSHLPLLAGQRGISFERIGQLEELRRRFQSLNRVLQRELGAPPKSGRELRDDLVPDCCPDILAKLDRVKEQRVNQTAHLIVAQVLGVQLRKHQTPESERTERDLHGEYEIMPGRKPVDFIVLEDLSKYLSSQARGRGENVRLMKWCHRQVTAKVKELCEPFGLPVLETPAAYSSKFCSRTGVAGFRGVEVTLKDRQSFPWSKRLEEDSAEVAELFGWLETASAGHKTKQPRCLLAPMSLGPLFIPVTSQAPIMQADINAAINLGLRAMAAPSVHEIHVRIRSEAKDGQFRVRAQSKREKARWGNEPPPIKMLVETQRVALAREGGHPNFFVDPLKAATFDRAEVEGLGLPVASGRGLWKSVRDAEWKRCREINLERMRRWGFIMEAPRTLQVSRKEDEDDLTF |
| 585 | Cas12 | MAVRSVKLKLLVPRDGSAESVRKRKALWATHQFVNDAAAAYAELLLEMRQEDVCRGTDDHGKDVIEPAAHWQAKLRARLAAKQLPPVAVAEALPLLKAFYGSRLIKSFVANDKGVAGTGNATDLNTWLSGLVDPASVAGEKTELRKQLLAELPLCEAADADFEGAARKMLAKSDAREALLEGPGTGVGWPAAYNANPTDSVWLDMLHKAAAKARLELADTTVSELKKLGVFPLLQAASSNRVFGSGVLNPFERMAAAQAAAALLPWETKRHEMRKRRDKFADQLNQWDTEFGASHATALAAIRAFEAEESERARRESLGNEGTGYRIGGRELRDAWTLLRDWLKGHSTATAAAREDKVRELQAKQGRSFGSHRLLSWLAKPAQQWLADHSAGDVVTRIAVRNARQRKLDTARTLPIWTGADAVKHPRFANFDPPNNTNQPGFDLRAGTQKGRLTLRLSLLTERADGLLLAQDHDFQLVPSRQMAEIVLHKDGKERALSWQSQDGIGRQVGDVGGSALLFSRDHAECLLERKQITRLERGAWPAALPVWFKLSLDIGAEHKALLKQRFKWGVWLNSALVTRNAKDAKGVPPPVGTRVLAVDLGLRSAATVSVWQVVDAATPVVAGKWRVPLSDTLSAVHERSAMLALPGEHVDAGVLAARRAANEKLAGLLAATSHLSTVFKLGRAEQGDRRRELLERLGEGDDRRARAAVATTAAERDGLRAVLGATQDAWAGAVAAVWRRLETDLAGAIAAYRKQQREDVQLRREARHGPGASQLPKQAAAERLLGGKSAWQIEYKERVRKLLTRWIMRQRPGDTAVRRLARKDLGKYCGGLLDHLTALKEDRAKTTADLIVQAARGRVRAHKDAHGRQQDRELWLAKYAPCDLIVMEDLGRYRFATDRPPSENRQLMQWTHREVFRLVQMQAEVEGIQVLETGAEFSSKFDARTWAPGVRCEPITKLWVERYRNGEMPWLADKADEWRREGIELAQLVPGQLLPTGSGEQFVAVSATGGLRVRHADLNAAQCIALRALTGHGTAFRLTARRLGDVFVSAKGLGKRPQGALWREFGSALPPAVVVLRPAGEVRYALRPFASARDAAAALGLQLGALRNVDATDAESDAEDGDLAELLAGADPDRATFFRDPSGDVHGGAWVQAKVFWAEVRRHVRLGLQAQGLLPAAARSSEPRQMQLPLAGALPGDDIPL |
| 586 | Cas12 | MSTQKNPFNQFTNLYELQKTLRFELRPVPETKKLLEKGEGKNLIQMDLEIDRLYEKEMKPMFNILHEKFINESLGLVKLDCKKLKKLENLLAEADKLRKQIKEGRKNKNNISEVEKRLKIIIGDNSQGKNKNGEIAVLQDELRVLIVKAFNLTADKWKKELNNKETLLPEKKGKRKIKIKKSGPKILQEENVLAILAYFNPDKADIIKKFAGFFTYFSGFNQNRANYYTVKALATGVANRAINRNFLIFLANRKDFARFKERLPRLAEFDNYFELENYEKYLSQTGIEEYNDQIGKIKQIVNLEHNQQQKDNKFQLKGLATLEKQIGCRTKKQREEGGDKSAPKFLEKVGLGFQVSQDDDGEYLIWECLNYINKELAGKLKSIKDNYQKFFADWRTGAYDLEKIWFRKEALNTISGRWFGGNNWFIIGKALALTGVGKFDKRENTYKIPEFVSLAEIKTAFEMLENGVNYDFKKSKKKKEGDDTDVVKYSADNLFKEEYKKKGLIKNSLFETMLAVWQSEIKRKFEQIFDGYKLEKDDVFGRKKGEWVEPFIENFQKVSQEKFDRGVKDENGRSIHTEVVKNLIEEGYLRLFQLTKYHNLDKKGERDPRPFDGNFYATLDEFWKDNIVVVYHKALQSTLTKKPYSEDKIKLNFENGSLLGGFSDGQERSKAGVVLKNKNKFYLGILIDRGFFRTDKANPVYDNAQNNEWERLILTNLKFQTLAGKGFLGKHGVSYGEMGKDNPMMAVEYLQKFIKLKYLDKYPALNEVAHKKYTIKKEFDADVKNALKDCFTMNFKPVDFGMIRQGLTESLFYLFEIVNKDISSQAKNGKNVHTLYWEALFGDQNLKKPILALNGGAEIFYRESQREKLEKKLDKSGKEVLDHKRYGQDKYFLHASITINYGQPKNIKFKEVINEKISQNADRVNIIGIDRGEKHLLYYSVVSPEGVLLEQGSFNQIETKNKVDIKAVKAEYGERGELKKVELVPTGKKVKYVDYQILLDYYEKKRNLARRDWQTIGKIKDLKDGYLSQTVHRIYQLILKYNAVVAMEDLNVEFKAKRAAKVEKSVYKNFEMALAKKLNHLILKDRRADEIGGALRAYQLTPAIPANDVGKFDKAKQWGIMFYVRANYTSTTDPLTGWRKHKYISNSEKIDNIQKFFSPGDGIQIDYDTEKQCFKFSYDHELEGGAKKHWELFACDGLERFYWDNRERQIKKYNLYEEFEKLLGGLRKEENINIQIDGVSEFRWKDLVFFWNLLNQIRNTDRSAQGDENDFLQSPVWSEKYNCFYDSRKAPNNMPNNGDANGAFNIARKGQLILERIKKCSDIPKFGNDNNGKNPENNYFISDADWDKFAQK |
| 587 | Cas12 | MAVKSIKVKLRLDDMPEIRAGLWKLHKEVNAGVRYYTEWLSLLRQENLYRRSPNGDGEQECDKTAEECKAELLERLRARQVENGHRGPAGSDDELLQLARQLYELLVPQAIGAKGDAQQIARKFLSPLADKDAVGGLGIAKAGNKPRWVRMREAGEPGWEEEKEKAETRKSADRTADVLRALADFGLKPLMRVYTDSEMSSVEWKPLRKGQAVRTWDRDMFQQAIERMMSWESWNQRVGQEYAKLVEQKNRFEQKNFVGQEHLVHLVNQLQQDMKEASPGLESKEQTAHYVTGRALRGSDKVFEKWGKLAPDAPFDLYDAEIKNVQRRNTRRFGSHDLFAKLAEPEYQALWREDASFLTRYAVYNSILRKLNHAKMFATFTLPDATAHPIWTRFDKLGGNLHQYTFLFNEFGERRHAIRFHKLLKVENGVAREVDDVTVPISMSEQLDNLLPRDPNEPIALYFRDYGAEQHFTGEFGGAKIQCRRDQLAHMHRRRGARDVYLNVSVRVQSQSEARGERRPPYAAVFRLVGDNHRAFVHFDKLSDYLAEHPDDGKLGSEGLLSGLRVMSVDLGLRTSASISVFRVARKDELKPNSKGRVPFFFPIKGNDNLVAVHERSQLLKLPGETESKDLRAIREERQRTLRQLRTQLAYLRLLVRCGSEDVGRRERSWAKLIEQPVDAANHMTPDWREAFENELQKLKSLHGICSDKEWMDAVYESVRRVWRHMGKQVRDWRKDVRSGERPKIRGYAKDVVGGNSIEQIEYLERQYKFLKSWSFFGKVSGQVIRAEKGSRFAITLREHIDHAKEDRLKKLADRIIMEALGYVYALDERGKGKWVAKYPPCQLILLEELSEYQFNNDRPPSENNQLMQWSHRGVFQELINQAQVHDLLVGTMYAAFSSRFDARTGAPGIRCRRVPARCTQEHNPEPFPWWLNKFVVEHTLDACPLRADDLIPTGEGEIFVSPFSAEEGDFHQIHADLNAAQNLQQRLWSDFDISQIRLRCDWGEVDGELVLIPRLTGKRTADSYSNKVFYTNTGVTYYERERGKKRRKVFAQEKLSEEEAELLVEADEAREKSVVLMRDPSGIINRGNWTRQKEFWSMVNQRIEGYLVKQIRSRVPLQDSACENTGDI |
| 588 | Cas12 | MSTQKNPFNQFTNLYELQKTLRFELRPVPETKKLLEKGEGKNLIQMDLEIDRLYEKEMKPMFNILHEKFINESLGLVKLDCKKLKKLENLLAEADKLRKQIKEGRKNKNNISEVEKRLKIIIGDNSQGKNKNGEIAVLQDELRVLIVKAFNLTADKWKKELNNKETLLPEKKGKRKIKIKKSGPKILQEENVLAILAYFNPDKADIIKKFAGFFTYFSGFNQNRANYYTVKALATGVANRAINRNFLIFLANRKDFARFKERLPRLAEFDNYFELENYEKYLSQTGIEEYNDQIGKIKQIVNLEHNQQQKDNKFQLKGLATLEKQIGCRTKKQREEGGDKSAPKFLEKVGLGFQVSQDDDGEYLIWECLNYINKELAGKLKSIKDNYQKFFADWRTGAYDLEKIWFRKEALNTISGRWFGGNNWFIIGKALALTGVGKFDKRENTYKIPEFVSLAEIKTAFEMLENGVNYDFKKSKKKKEGDDTDVVKYSADNLFKEEYKKKGLIKNSLFETMLAVWQSEIKRKFEQIFDGYKLEKDDVFGRKKGEWVEPFIENFQKVSQEKFDRGVKDENGRSIHTEVVKNLIEEGYLRLFQLTKYHNLDKKGERDPRPFDGNFYATLDEFWKDNIVVVYHKALQSTLTKKPYSEDKIKLNFENGSLLGGFSDGQERSKAGVVLKNKNKFYLGILIDRGFFRTDKANPVYDNAQNNEWERLILTNLKFQTLAGKGFLGKHGVSYGEMGKDNPMMAVEYLQKFIKLKYLDKYPALNEVAHKKYTIKKEFDADVKNALKDCFTMNFKPVDFGMIRQGLTESLFYLFEIVNKDISSQAKNGKNVHTLYWEALFGDQNLKKPILALNGGAEIFYRESQREKLEKKLDKSGKEVLDHKRYGQDKYFLHASITINYGQPKNIKFKEVINEKISQNADRVNIIGIDRGEKHLLYYSVVSPEGVLLEQGSFNQIETKNKVDIKAVKAEYGERGELKKVELVPTGKKVKYVDYQILLDYYEKKRNLARRDWQTIGKIKDLKDGYLSQTVHRIYQLILKYNAVVAMEDLNVEFKAKRAAKVEKSVYKNFEMALAKKLNHLILKDRRADEIGGALRAYQLTPAIPANDVGKFDKAKQWGIMFYVRANYTSTTDPLTGWRKHKYISNSEKIDNIQKFFSPGDGIQIDYDTEKQCFKFSYDHELEGGAKKHWELFACDGLERFYWDNRERQIKKYNLYEEFEKLLGGLRKEENINIQIDGVSEFRWKDLVFFWNLLNQIRNTDRSAQGDENDFLQSPVWSEKYNCFYDSRKAPNNMPNNGDANGAFNIARKGQLILERIKKCSDIPKFGNDNNGKNPENNYFISDADWDKFAQK |
| 589 | Cas12 | MAIRSIKLKLKTHTGPEAQNLRKGIWRTHRLLNEGVAYYMKMLLLFRQESTGERPKEELQEELICHIREQQQRNQADKNTQALPLDKALEALRQLYELLVPSSVGQSGDAQIISRKFLSPLVDPNSEGGKGTSKAGAKPTWQKKKEANDPTWEQDYEKWKKRREEDPTASVITTLEEYGIRPIFPLYTNTVTDIAWLPLQSNQFVRTWDRDMLQQAIERLLSWESWNKRVQEEYAKLKEKMAQLNEQLEGGQEWISLLEQYEENRERELRENMTAANDKYRITKRQMKGWNELYELWSTFPASASHEQYKEALKRVQQRLRGRFGDAHFFQYLMEEKNRLIWKGNPQRIHYFVARNELTKRLEEAKQSATMTLPNARKHPLWVRFDARGGNLQDYYLTAEADKPRSRRFVTFSQLIWPSESGWMEKKDVEVELALSRQFYQQVKLLKNDKGKQKIEFKDKGSGSTFNGHLGGAKLQLERGDLEKEEKNFEDGEIGSVYLNVVIDFEPLQEVKNGRVQAPYGQVLQLIRRPNEFPKVTTYKSEQLVEWIKASPQHSAGVESLASGFRVMSIDLGLRAAAATSIFSVEESSDKNAADFSYWIEGTPLVAVHQRSYMLRLPGEQVEKQVMEKRDERFQLHQRVKFQIRVLAQIMRMANKQYGDRWDELDSLKQAVEQKKSPLDQTDRTFWEGIVCDLTKVLPRNEADWEQAVVQIHRKAEEYVGKAVQAWRKRFAADERKGIAGLSMWNIEELEGLRKLLISWSRRTRNPQEVNRFERGHTSHQRLLTHIQNVKEDRLKQLSHAIVMTALGYVYDERKQEWCAEYPACQVILFENLSQYRSNLDRSTKENSTLMKWAHRSIPKYVHMQAEPYGIQIGDVRAEYSSRFYAKTGTPGIRCKKVRGQDLQGRRFENLQKRLVNEQFLTEEQVKQLRPGDIVPDDSGELFMTLTDGSGSKEVVFLQADINAAHNLQKRFWQRYNELFKVSCRVIVRDEEEYLVPKTKSVQAKLGKGLFVKKSDTAWKDVYVWDSQAKLKGKTTFTEESESPEQLEDFQEIIEEAEEAKGTYRTLFRDPSGVFFPESVWYPQKDFWGEVKRKLYGKLRERFLTKAR |
| 590 | Cas12 | MRKKLFKGYILHNKRLVYTGKAAIRSIKYPLVAPNKTALNNLSEKIIYDYEHLFGPLNVASYARNSNRYSLVDFWIDSLRAGVIWQSKSTSLIDLISKLEGSKSPSEKIFEQIDFELKNKLDKEQFKDIILLNTGIRSSSNVRSLRGRFLKCFKEEFRDTEEVIACVDKWSKDLIVEGKSILVSKQFLYWEEEFGIKIFPHFKDNHDLPKLTFFVEPSLEFSPHLPLANCLERLKKFDISRESLLGLDNNFSAFSNYFNELFNLLSRGEIKKIVTAVLAVSKSWENEPELEKRLHFLSEKAKLLGYPKLTSSWADYRMIIGGKIKSWHSNYTEQLIKVREDLKKHQIALDKLQEDLKKVVDSSLREQIEAQREALLPLLDTMLKEKDFSDDLELYRFILSDFKSLLNGSYQRYIQTEEERKEDRDVTKKYKDLYSNLRNIPRFFGESKKEQFNKFINKSLPTIDVGLKILEDIRNALETVSVRKPPSITEEYVTKQLEKLSRKYKINAFNSNRFKQITEQVLRKYNNGELPKISEVFYRYPRESHVAIRILPVKISNPRKDISYLLDKYQISPDWKNSNPGEVVDLIEIYKLTLGWLLSCNKDFSMDFSSYDLKLFPEAASLIKNFGSCLSGYYLSKMIFNCITSEIKGMITLYTRDKFVVRYVTQMIGSNQKFPLLCLVGEKQTKNFSRNWGVLIEEKGDLGEEKNQEKCLIFKDKTDFAKAKEVEIFKNNIWRIRTSKYQIQFLNRLFKKTKEWDLMNLVLSEPSLVLEEEWGVSWDKDKLLPLLKKEKSCEERLYYSLPLNLVPATDYKEQSAEIEQRNTYLGLDVGEFGVAYAVVRIVRDRIELLSWGFLKDPALRKIRERVQDMKKKQVMAVFSSSSTAVARVREMAIHSLRNQIHSIALAYKAKIIYEISISNFETGGNRMAKIYRSIKVSDVYRESGADTLVSEMIWGKKNKQMGNHISSYATSYTCCNCARTPFELVIDNDKEYEKGGDEFIFNVGDEKKVRGFLQKSLLGKTIKGKEVLKSIKEYARPPIREVLLEGEDVEQLLKRRGNSYIYRCPFCGYKTDADIQAALNIACRGYISDNAKDAVKEGERKLDYILEVRKLWEKNGAVLRSAKFL |
| 591 | Cas12 | MSTQKNPFNQFTNLYELQKTLRFELRPVPETKKLLEKGEGKNLIQMDLEIDRLYEKEMKPMFNILHEKFINESLGLVKLDCKKLKKLENLLAEADKLRKQIKEGRKNKNNISEVEKRLKIIIGDNSQGKNKNGEIAVLQDELRVLIVKAFNLTADKWKKELNNKETLLPEKKGKRKIKIKKSGPKILQEENVLAILAYFNPDKADIIKKFAGFFTYFSGFNQNRANYYTVKALATGVANRAINRNFLIFLANRKDFARFKERLPRLAEFDNYFELENYEKYLSQTGIEEYNDQIGKIKQIVNLEHNQQQKDNKFQLKGLATLEKQIGCRTKKQREEGGDKSAPKFLEKVGLGFQVSQDDDGEYLIWECLNYINKELAGKLKSIKDNYQKFFADWRTGAYDLEKIWFRKEALNTISGRWFGGNNWFIIGKALALTGVGKFDKRENTYKIPEFVSLAEIKTAFEMLENGVNYDFKKSKKKKEGDDTDVVKYSADNLFKEEYKKKGLIKNSLFETMLAVWQSEIKRKFEQIFDGYKLEKDDVFGRKKGEWVEPFIENFQKVSQEKFDRGVKDENGRSIHTEVVKNLIEEGYLRLFQLTKYHNLDKKGERDPRPFDGNFYATLDEFWKDNIVVVYHKALQSTLTKKPYSEDKIKLNFENGSLLGGFSDGQERSKAGVVLKNKNKFYLGILIDRGFFRTDKANPVYDNAQNNEWERLILTNLKFQTLAGKGFLGKHGVSYGEMGKDNPMMAVEYLQKFIKLKYLDKYPALNEVAHKKYTIKKEFDADVKNALKDCFTMNFKPVDFGMIRQGLTESLFYLFEIVNKDISSQAKNGKNVHTLYWEALFGDQNLKKPILALNGGAEIFYRESQREKLEKKLDKSGKEVLDHKRYGQDKYFLHASITINYGQPKNIKFKEVINEKISQNADRVNIIGIDRGEKHLLYYSVVSPEGVLLEQGSFNQIETKNKVDIKAVKAEYGERGELKKVELVPTGKKVKYVDYQILLDYYEKKRNLARRDWQTIGKIKDLKDGYLSQTVHRIYQLILKYNAVVAMEDLNVEFKAKRAAKVEKSVYKNFEMALAKKLNHLILKDRRADEIGGALRAYQLTPAIPANDVGKFDKAKQWGIMFYVRANYTSTTDPLTGWRKHKYISNSEKIDNIQKFFSPGDGIQIDYDTEKQCFKFSYDHELEGGAKKHWELFACDGLERFYWDNRERQIKKYNLYEEFEKLLGGLRKEENINIQIDGVSEFRWKDLVFFWNLLNQIRNTDRSAQGDENDFLQSPVWSEKYNCFYDSRKAPNNMPNNGDANGAFNIARKGQLILERIKKCSDIPKFGNDNNGKNPENNYFISDADWDKFAQK |
| 592 | Cas12 | MAQASSTPAVSPRPRPRYREERTLVRKLLPRPGQSKQEFRENVKKLRKAFLQFNADVSGVCQWAIQFRPRYGKPAEPTETFWKFFLEPETSLPPNDSRSPEFRRLQAFEAAAGINGAAALDDPAFTNELRDSILAVASRPKTKEAQRLFSRLKDYQPAHRMILAKVAAEWIESRYRRAHQNWERNYEEWKKEKQEWEQNHPELTPEIREAFNQIFQQLEVKEKRVRICPAARLLQNKDNCQYAGKNKHSVLCNQFNEFKKNHLQGKAIKFFYKDAEKYLRCGLQSLKPNVQGPFREDWNKYLRYMNLKEETLRGKNGGRLPHCKNLGQECEFNPHTALCKQYQQQLSSRPDLVQHDELYRKWRREYWREPRKPVFRYPSVKRHSIAKIFGENYFQADFKNSVVGLRLDSMPAGQYLEFAFAPWPRNYRPQPGETEISSVHLHFVGTRPRIGFRFRVPHKRSRFDCTQEELDELRSRTFPRKAQDQKFLEAARKRLLETFPGNAEQELRLLAVDLGTDSARAAFFIGKTFQQAFPLKIVKIEKLYEQWPNQKQAGDRRDASSKQPRPGLSRDHVGRHLQKMRAQASEIAQKRQELTGTPAPETTTDQAAKKATLQPFDLRGLTVHTARMIRDWARLNARQIIQLAEENQVDLIVLESLRGFRPPGYENLDQEKKRRVAFFAHGRIRRKVTEKAVERGMRVVTVPYLASSKVCAECRKKQKDNKQWEKNKKRGLFKCEGCGSQAQVDENAARVLGRVFWGEIELPTAIP |
| 593 | Cas12 | MAVRSVKLKLLVPRDGSAESVRKRKALWATHQFVNDAAAAYAELLLEMRQEDVCRGTDDHGKDVIEPAAHWQAKLRARLAAKQLPPVAVAEALPLLKAFYGSRLIKSFVANDKGVAGTGNATDLNTWLSGLVDPASVAGEKTELRKQLLAELPLCEAADADFEGAARKMLAKSDAREALLEGPGTGVGWPAAYNANPTDSVWLDMLHKAAAKARLELADTTVSELKKLGVFPLLQAASSNRVFGSGVLNPFERMAAAQAAAALLPWETKRHEMRKRRDKFADQLNQWDTEFGASHATALAAIRAFEAEESERARRESLGNEGTGYRIGGRELRDAWTLLRDWLKGHSTATAAAREDKVRELQAKQGRSFGSHRLLSWLAKPAQQWLADHSAGDVVTRIAVRNARQRKLDTARTLPIWTGADAVKHPRFANFDPPNNTNQPGFDLRAGTQKGRLTLRLSLLTERADGLLLAQDHDFQLVPSRQMAEIVLHKDGKERALSWQSQDGIGRQVGDVGGSALLFSRDHAECLLERKQITRLERGAWPAALPVWFKLSLDIGAEHKALLKQRFKWGVWLNSALVTRNAKDAKGVPPPVGTRVLAVDLGLRSAATVSVWQVVDAATPVVAGKWRVPLSDTLSAVHERSAMLALPGEHVDAGVLAARRAANEKLAGLLAATSHLSTVFKLGRAEQGDRRRELLERLGEGDDRRARAAVATTAAERDGLRAVLGATQDAWAGAVAAVWRRLETDLAGAIAAYRKQQREDVQLRREARHGPGASQLPKQAAAERLLGGKSAWQIEYKERVRKLLTRWIMRQRPGDTAVRRLARKDLGKYCGGLLDHLTALKEDRAKTTADLIVQAARGRVRAHKDAHGRQQDRELWLAKYAPCDLIVMEDLGRYRFATDRPPSENRQLMQWTHREVFRLVQMQAEVEGIQVLETGAEFSSKFDARTWAPGVRCEPITKLWVERYRNGEMPWLADKADEWRREGIELAQLVPGQLLPTGSGEQFVAVSATGGLRVRHADLNAAQCIALRALTGHGTAFRLTARRLGDVFVSAKGLGKRPQGALWREFGSALPPAVVVLRPAGEVRYALRPFASARDAAAALGLQLGALRNVDATDAESDAEDGDLAELLAGADPDRATFFRDPSGDVHGGAWVQAKVFWAEVRRHVRLGLQAQGLLPAAARSSEPRQMQLPLAGALPGDDIPL |
| 594 | Cas12 | MNRIYQGRVSKIEIKDSEGNFRNVPVGSPDTCPLWRHHRIFQDAVNYYLVALGALAGTGSENAFVGLGSKDRVIHDLYSRLFDSWERFPRDMHGASSLRDSLRRTLPGLSERASLQDAFDAILSGNEANARERVLSLLSLIQDLGGDIQKGSKRYFPFFCEPATKATFPRARVGLLKVEGKDFVPRLLWSSDLEIAPDQVVEQLKFEYFANPNESVQPIEGNEARVRLIEALDNPQLGIELPIEILSDLRKRVHLIETDIRIPRYFFGGAGAELRKFRLDLFLIAAYVTPDPSILRALRNSFKEPSASKSSKKKDETEEVENLLRSLGDDPLILARGERGFVFPSFTSLPTWVGANAQKPIWRDFDIAAFAEALKSLNQFTAKTEEREEKLKKAEETLHYMLGISDAIPRSSDSETEEQAPSRPGKDPRWPLVAQLEKELGENLSEGTWQLSRSAMRGLRDIIGLWRKHPGASVVTLQKDVKTYQADEKHKREIGSVQLFLLLCEERYHALWQTETDDERGDESEENDDPARILSDAIEVHQIRREVERFREPIRLTPAEPVFSRRLFMFSDLTDKLAKVKFGETTEENSEVKSQFVEAAIALKEGENLKEARVRITFSAPRLHRDELLGGAESRWLQPITAALGFSNPAPSVKFDSAVALMPDHMDDGRIRHLLNFPVNFDSAWLHQSIGKADLWKSQFNGTKDKNLHLHWAGTARDTTRKNTWWENRTIIENGFTVLSNDLGQRSAGAWALLKVTCSRPDTKHPVRSIGHDGTREWFATVLATGIHRLPGEDQRILKNGKWATEQSGKKGRNATFSEYEAACVLAKNLGCESVENWLGMSGEKSYPALNDQLVKIANRRITRLGTYHRWSCFSPEKFEDPARRANVIGGQLAELSAYQDENVTVSADILKSGDFEGFRHRAGAAFEALRTELEVHLVNLANLTAPLRQKVWSWQKRPDSSGYGDLLMVDLDDCHPKIRGQRGLSMARLEQLEGLRRLFLRYNRSLDRSPGIPAKFGREDVGRTSGEPCQALLVKIDRMKEQRVNQTAHLILAQALGVRLCPHRIEENERKSRDLHGEYEKIPGREPVDFIVIEDLSRYLSSQGRAPSENSRLMKWAHRAVRDKLKMLAEEPFGIPVVETVPAYSSRFHALNGQAGSRLHELHELEAYQQQSLINLAAKTDFQNRDRSKAAGELFEQFQALAKLNERRRAEGKKVPRTLYYPKSGGPLFLASRDGDTIHADVNAAINLGLRAIAAPACIDIHRRLRATKEKEVYRPRVGNAREKSAFSKDDIIQPSGAPSKKFASSSSPNFFYEPEDLKQANGEPLFDRAMFGEYSLVSGVSLWSMVNNAIYIRCVELNRTRLHGKDPDDQIPM |
| 595 | Cas12 | MADDLSTQRAYTLRLQGTDPEDQSWRDALWMTHEAVNAGGRAFGDWLLTLRGGIAHELADTPVKGKKDITDELRKKRRILLALSWLSVESRRGAPDKFIVAGGEEPAGSRNEKVLQALKEILKRRGLSAEESESWMSDCRASLSAAIRDDAVWVNRSAAFDDAQVRIGASLTREDIWDMLDPFFGSREAYLTPAKKKKEDEDSSEGTGEEKAKDLVQKAGQWLSSRFGTGKGANFDAMAEVYSKISEWAGTAQEGVSGKEGIKNLADALAAFSPVSQNLEGVLKLISGPGYKSATRNLLGELDSLPVVSRDHLSALHEKAAEDTVKCKESTGTKGRRPYADAILNDVEKRCGFTYLTDSDNRSVSILDTSEFPSDYKWGTARHSEFAVILDHAARRISVAHSWIKLAEAERDRCEEDAAKVYDLPDKVKEWLDTFCSNRSDISGAQGEGYRIRRKAIEGWKEVVASWGRSSCITAEDRVAAARALQDDPEIDKFGDIQLFEILAQDEALCVWHKDGDVAKSPDAQMLIDYVLASDAESKKRRFKVPAYRHPDALLHPIFCDFGNSRWDITYDIHGARGKKKAKRGSKKEEAMPRGVAMKLWTGSDVLSVSLRWQSKKLAADLALDQEAEEVTDTAAVSRADRLGRAAAGIDRGAGVTIAGLFEEAHWNGRLQAPRQQLEAIAAVRDNQKLSSEERERRIAFMKDRIRWLVTFSAKLRPQGPWHSYAPTQGLQSDPKYWPHSEINKKRKGQAKLILSRLPGLRILSVDLGHRFAAACAVWETMSSEAIQEACRLANHQLPAPADLYLHLKRTVQKNLIDGEKTVEESTVYRRIGADRLPDGTAHPAPWARLDRQFLIKLQGEEKVREASNEEVWQVHLMESALGLSFPLIDRLVYAGWGGTEKQAARLEALREKGWKPTGTPADQDEEGGGYKPSLAVDELMFSAVRTLRLALKYHGDRARIAFALTADYKPMPGDTRYYFSEAKDRSSGADAAEREAKHKDYLLDMLLLWHDLAFSRKWRDEEAKELWNLHIAALPGYQAPAAPIQEEAGQGRKKAREEARAKMTPAAEALLADGTLREKLHGLWKERWEKDDAQWKKHLRWMKDGILPRGGRAATPSIRYVGGLSLTRLATLTEFRRKVQVGFYTRLFPSGEKREIKEAFGQTALDALERLREQRVKQLASRIAEAALGAGRVSRTALKQDPKRPEARVDAACHAVIIENLEHYRPEETRTRRENRGLMNWASSKVKKYLSEACQLHGLFLREVPAGYTSRQDSRTGAPGMRCQDVTVKTFLNSPFWQKQCVQAQKNKSTARDRFLCALKEAVAQGGMEEEKKMGPIRVPVPGGEVFVSADAASPAAKGLQADLNAAANIGLRALLDPDWPGKWWYVPCDRKTAYPAKEKVEGSAAVDVKQALPFVLPEEKENKGKTKGGKKGKGEVMNLWRDVSAEPLMTGQWLDYTAYRKEVENRVIQVLTAQLKARNPLRFGNLGDEEEIPY |
| 596 | Cas12 | MAIRSIKLKLKTHTGPEAQNLRKGIWRTHRLLNEGVAYYMKMLLLFRQESTGERPKEELQEELICHIREQQQRNQADKNTQALPLDKALEALRQLYELLVPSSVGQSGDAQIISRKFLSPLVDPNSEGGKGTSKAGAKPTWQKKKEANDPTWEQDYEKWKKRREEDPTASVITTLEEYGIRPIFPLYTNTVTDIAWLPLQSNQFVRTWDRDMLQQAIERLLSWESWNKRVQEEYAKLKEKMAQLNEQLEGGQEWISLLEQYEENRERELRENMTAANDKYRITKRQMKGWNELYELWSTFPASASHEQYKEALKRVQQRLRGRFGDAHFFQYLMEEKNRLIWKGNPQRIHYFVARNELTKRLEEAKQSATMTLPNARKHPLWVRFDARGGNLQDYYLTAEADKPRSRRFVTFSQLIWPSESGWMEKKDVEVELALSRQFYQQVKLLKNDKGKQKIEFKDKGSGSTFNGHLGGAKLQLERGDLEKEEKNFEDGEIGSVYLNVVIDFEPLQEVKNGRVQAPYGQVLQLIRRPNEFPKVTTYKSEQLVEWIKASPQHSAGVESLASGFRVMSIDLGLRAAAATSIFSVEESSDKNAADFSYWIEGTPLVAVHQRSYMLRLPGEQVEKQVMEKRDERFQLHQRVKFQIRVLAQIMRMANKQYGDRWDELDSLKQAVEQKKSPLDQTDRTFWEGIVCDLTKVLPRNEADWEQAVVQIHRKAEEYVGKAVQAWRKRFAADERKGIAGLSMWNIEELEGLRKLLISWSRRTRNPQEVNRFERGHTSHQRLLTHIQNVKEDRLKQLSHAIVMTALGYVYDERKQEWCAEYPACQVILFENLSQYRSNLDRSTKENSTLMKWAHRSIPKYVHMQAEPYGIQIGDVRAEYSSRFYAKTGTPGIRCKKVRGQDLQGRRFENLQKRLVNEQFLTEEQVKQLRPGDIVPDDSGELFMTLTDGSGSKEVVFLQADINAAHNLQKRFWQRYNELFKVSCRVIVRDEEEYLVPKTKSVQAKLGKGLFVKKSDTAWKDVYVWDSQAKLKGKTTFTEESESPEQLEDFQEIIEEAEEAKGTYRTLFRDPSGVFFPESVWYPQKDFWGEVKRKLYGKLRERFLTKAR |
| 597 | Cas12 | MPVRSINLKIVISRNTQGEKSRQSIWTTHAAVNDAVRYYEEQLLIMRGLGYHISDKDVVSKESIQQERLSRIRRAQLENGLPEPLGTDAELNSLVRKFYEFIVPSSVKEDGNAQQANGFLSPLTDPISIGYLSIFEKLGTIPDWVGQLKAGDPQAVENAKKWSATSAGIKRLSETGAPPKWKKLFLTGDPSWPQSFSEDIDKKIKEIEGAPKVICQLMEMGVLPLFPAYFADKLEGSDGSLSRWDRLAFRLAVGHMLSWESWCIKSAEDHFERKRRVESFSEKHTTPSLIICFETLEKYQKERQEKELGQNRSLPMQRPFRITRRQIRGWEDLRDKWLKNTTRTYDSLKSIASKEQTKKGGRFGDPHLFLWLAKPENHAVWDADEDALSIFAKMNAMRGLLERSRETAYMTLPDPIEHPRSIQWEAEGGSNFKNYVITHSPVEGLHVQLPLLCKSESGKLIDQTFEFPLAPSDQFKVAQISKTKSEVTITHQSVLDEEYRSKVGAADLLMDWPYLKNRRFESVEHGDIGPVFLKLSLDIERILPDGWTPKRPQAISHFSSASGNSKHKLSVVSGLRVLSVDLGIRSFGACSVFELSEHKPTSGMSFEIEGLNLWANHERSFMLNLPDEDVGNKGRQLQKTKDAELRAMRRVLGRYRKIYALAGIDPEDRKDILELLCQDQDIFEFERTIYKGLVTSTSVSQPLWEGKIKESLKALRNAFGRKVREWRRANRLNSNLKYAGKTMWAIQHLEDTRRFLHSWSHLGRFSGEIRRADRVKRGVFATRLLQHLDSVKRDRLKTGADLLVQSARGFLRDNQGNWKKSYAPCQVILFEDLSRYLMQTDRPRRENSQLMKWSHRSIPLEVAMQGELYGIHVCDTSAAFSSRYHARLATPGIRCHALRKEDLSNQFLIESLQKENPDIDFGICKAGDLIPRGGGEIFVSCDGNGGISRIHADINAAQNLQRRFWLRHGEAIRIPARKITLKGDEIWVPRSIGKRLQGAMSGCGYLIPTGHESGSCRWERITASKWESISRSSVAQKEEVNEDLLDIALLEEEALELSNEYTTFFRDPSGITLPSDLWFPMKTFWGMTRAKIKSAIKQ |
| 598 | Cas12 | MTVKSIKVKLRLDNMPEIRAGLWKLHTEVNAGVRYYTEWLSLLRQENLYRRSPNGDGEQECYKTAEECKVELLERLRARQVENGHRDPAGSDDELLQLARQLYELLVPQAIGAKGDAQQIARKFLSPLADKDAVGGLGIAKAGNKPRWVRMRDAGEPGWEEEKAKAEARKSTDRTADVLRALADFGLKPLMRVYTDSDMSSVQWKPLRKGQAVRTWDRDMFQQAIERMMSWESWNQRVGEAYAKLVEQKSRFEQKNFVGQEHLVQLVNQLQQDMKEASHGLESKEQTAHYLTGRALRGSDKVFEKWEKLDPDAPFDLYDTEIKNVQRRNTRRFGSHDLFAKLAEPKYQALWREDASFLTRYAAYNSILRKLNHAKMFATFTLPDATAHPIWTRFDKLGGNLHQYTFLFNEFGEGRHAIRFQKLLTIEHGVAKEVDDVTVPISMSAQLDDLLPGESNEPTELSFRDHGTDQHFTGEFGGAKIQYRRDQLDHVHRRRGARDVYLNLSVRVQSQSEARGERRPPYAAVFRLVGDTHRAFAHFDKLSNYLAEHPDDGKLGSEGLLSGLRVMSVDLGLRTSASISIFRVARKDELKPNSEGRVPFFFPIKGNDNLVAVHERSQLLKLPGETESKDLRAIREERQRILRQLRTQLAYLRLLVRCGSEDVGRRERSWAKLIEQSVDAANHMTPDWREAFEGELQKLKSLYGICGDREWTEAVYESVRRVWRHMGKQVRDWRKDVRSGERPKIRGYQKDVVGGNSIEQIEYLERQYKFLKSWSFFGKVSGQVIRAEKGSRFATTLREHIDHAKEDRLKKLADRIIMEALGYVYALDAERGKGTWVAKYPPCQLILLEELSEYRFNNDRPPSENNQLMQWSHRGVFQELLNQAQVHDLLVGTMYAAFSSRFDARTGAPGIRCRRVPARCAREQNPEPFPWWLNKFVAEHKLDGCPLRADDLIPTGEGEFFVSPFSAEEGDFHQIHADLNAAQNLQRRLWSDFDISQIRLRCDWGEVDGEPVLIPRLTGKRTADSYGNKVFYTNTGVTYYERERGKKRRKAFAQEELSEEEAELLVEADEAREKSVVLMRDPSGIINRGDWTRQKEFWSMVNQRIEGYLVKQIRSRVCLPESACENTGDI |
| 599 | Cas12 | MKNFQDFTNLYELSKTLRFELKPIGGTKKLIEEKNILKLDKKKRENYEKVKPYFNKIHQEFINFALRNPNFDFSQFEEKYLNWLKDKKNKDLLKEKESIDKIFLEKIGKLFENSVKDFLKENGFESIVKEEDQNLKFFRRKEIFEVLQEKYGSELETQMVNKDGEIKSIFNGWEKWLGYFDKFFNTRDNFYKTDGTSTAIATRIIKDNLKIFLENIVAFGKIKNKKIDFSEVEKNFSVSIDTFFEINNFNNCFLQDGIDFYNKVIGGETLENGEKLKGLNEIINKYRQDTGEKIPYFKKLQKQILSEKDGVFIDKIEDDGGFYEVLKNFYKNAAEKEGFLKNIFENFYTISDKNLEKIYFNKIAFNTISHKFGSALEFERILYEEMKKEKADGIKFEKKENKYKFPDFIQIIFIKRSLENYDSENLFWKERYYKSEENVDGFLEKNNNNLWGQFCKILNFEFLNILKRRIIDEAGEEYEVGFEISKNILGEKLENFELNQENKGIIKDFADYSLALYSFGKYFAVEKGRNWDLNIDISDDFYGGEDGYIEKFYNTGYDEIVKPYNLMRNYISKKPWEDNKKWKINFETSSLLSGWDKNLESNGSYIFQKGNKYYLGIINGSKPAKEILEKLYSGDGEKIKRFIYDFQKPDNKNTPRMFIRSKKDSFSPAVEKYNLPINDILEIYDNGLFKTENKGNPNYKESLRKLIDYFKLGFSRHESFKHFNFVWKDSKSYENIADFYRDVEKSCYKIDFEFLNFEELKKLTFEKHLYLFQIYNKDFELDESLQEKGYNFKGEGQKNIHTKYFEALFLEENISRKSGAVFKLSGGGEVFFRKKSIKAKKEKRNSVEVIKNKRYTECKYFLHFPIQVNFKEEISGNFNQEINKFLANNPDINVIGIDRGEKHLAYFSVINQKGEILESGSFNKIENYNKNGEKLLFPEREIKEIHKDGSLIDLELVETGRKVDYVDYKLLLEYKERKRLLQRQSWKEVEQIKDLKKGYISALVRKIADLIIKHNAIVIFEDLNFRFKQIRGGIEKSIYQQLEKALIDKLNFLVNKNEINLEKAGSILKAYQLTVPVDSLKEIGKQTGVIFYTEAAYTSKIDPITGWRPNLYLKKNNSKINKENILKFDNIVFNSKENRFEFTYDLKKFFGKDSKFPAKTVNTVCSCVERFKWNRNLNNNKGGYIHYENLTDGKLANKEQKEDEFSNFKELFEKYFIDINGNILEQIKNLDTKNNEKFFSSFIDLFTLVCQIRNTNQNAKGDENDFILSPVEPFFDSRKSQNFGKSLPKNGDENGAFNIARKGLIILNRISENPEKPDLLIFNADWDNFARNI |
| 600 | Cas12 | MAQAEAPRRLRAYKFALDPTEAQLREFEQHAGSARWAYNHANAILSRYSDTLRNRWNAWIAQHHGLSREQLYALPDRERTAIQAAARAAVKAENAQLAAELRIIDDHRKRVTHKGKPSVEPGEQPAEDAPERAYQLWRERVELARLHAEDPQAYRAERKRILDEIRPLVNATKRKLIEQGAYRPTAMDISTLWREIRDLPPDEGGSPWWPEVSIYAFTSGFAHAETAWKNYLESLAGRRAGRPVGKPRFKKKRRSRRSFTLYGSVKLVTYRRIQVPSIGSVRLHGSAKRLHRALERRGGIIKSITISQGGHRWYASVLVDELDITPGRETQRGPSRRQRDRGAVGVDLGVHHLVALSDPNEKTLDNPRHLRKARKRLLKAQRAMSRRRGPDKRTGQEPSRRWVKARNRVARLHHELAVRRAGHLHEITKRLATSYELVAIEDLNVAGMTRSARGTIDQPGRGVRAKAGLNRSILDTSPAEFRRQLQYKASWYGATVAVIDRWAPTSRTCSSCGAVKAKLSLAERTFFCEHCGMELDRDINAARNILAFAQSAYPGEGKALNACGGSVSPGSQSVVQAGADEAGRPARKPRRSSRGSDPPATPTTRA |
| 601 | Cas12 | MKRIAKFRHDKPVKREAWSKGYRVHKNRIINKVTRSIKYPLVVKDEWKKRLIDDAAHDYRWLVGPINYSDWCRDPNQYSILEFWIDFLCVGGVFQSSHSNICRLAIQLSGGSVFEQEWKDLSPFVRANLIQGIKPAEFIGFLTAEFRSSSNPKNFISKFFEGSNEDLESLTNEFASIVDFIKAKDISLLRKSLPSCKKIAPNLWEKAVGSHSTNELLKLLTKYTRVMLVAEPSHSDRVFSQTVLQSNDQDDPELTGPLPSHKVGKASYLFIPEFIREVNLDKISKLDLSAKSKLAVEQVKKLSELTSDFKQIENQSEAYFGLSTSFNELSNFLGILIRTLRNAPEAILKDQIALCAPLDKDILKITLDWLCDRAQALPENPRFETNWAEYRSYLGGKIKSWFSNYENFFEIPQAASSQQNNNREKKLGNRSAIRALNLKKEAFEKARETFKGDKGTLEKIDLAYRLLGSISPEVLQCDEGLKLYQQFNDELLVLNETINQKFQDAKRDIKAKKEKESFEKLQRNLSSPLPRIPEFFGERAKKGYQKARVSPKLARHLLECLNDWLARFAKVEESAFSEKEFQRILDWLRTSDFLPVFIRKSKDPPSWLRYIARVATGKYYFWVSEYSRKRVQIIDKPIAQNPLKELISWFLLNKDAFSRDNELFKGLSSKMVTLARIMAGILRDRGEGLKELQAMTSKLDNIGLLHPSFSVPVTDSLKDAAFYRAFFSELEGLLNIGRSRLIIERITLQSQQSKNKKTRRPLMPEPFINEDKEVFLAFPKFETKNKVKGTRVVYNSPDEVNWLLSPIRSSKGQLSFMFRCLSEDAKIMTTSGGCSYIVEFKKLLEAQEEVLSIHDCDIIPRAFVSIPFTLERESEETKPDWKPNRFMGVDIGEYAVAYCVIEKGTDSIEILDCGIVRNGAHRVLKEKVDRLKRRQRSMTFGAMDTSIAAARESLVGNYRNRLHAIALKHGAKLVYEYEVSAFESGGNRIKKVYETLKKSDCTGETEADKNARKHIWGETNAVGDQIGAGWTSQTCAKCGRSFGADLKAGNFGVAVPVPEKVEDSKGHYAYHEFPFEDGLKVRGFLKPNKIISDQKELAKAVHAYMRPPLVALGKRKLPKNARYRRGNSSLFRCPFSDCGFTADADIQAAYNIAVKQLYKPKKGYPKERKWQDFVILKPKEPSKLFDKQFYRPN |
| 602 | Cas12 | MAQAEAPRRLRAYKFALDPTEAQLREFEQHAGSARWAYNHANAILSRYSDTLRNRWNAWIAQHHGLSREQLYALPDRERTAIQAAARAAVKAENAQLAAELRIIDDHRKRVTHKGKPSVEPGEQPAEDAPERAYQLWRERVELARLHAEDPQAYRAERKRILDEIRPLVNATKRKLIEQGAYRPTAMDISTLWREIRDLPPDEGGSPWWPEVSIYAFTSGFAHAETAWKNYLESLAGRRAGRPVGKPRFKKKRRSRRSFTLYGSVKLVTYRRIQVPSIGSVRLHGSAKRLHRALERRGGIIKSITISQGGHRWYASVLVDELDITPGRETQRGPSRRQRDRGAVGVDLGVHHLVALSDPNEKTLDNPRHLRKARKRLLKAQRAMSRRRGPDKRTGQEPSRRWVKARNRVARLHHELAVRRAGHLHEITKRLATSYELVAIEDLNVAGMTRSARGTIDQPGRGVRAKAGLNRSILDTSPAEFRRQLQYKASWYGATVAVIDRWAPTSRTCSSCGAVKAKLSLAERTFFCEHCGMELDRDINAARNILAFAQSAYPGEGKALNACGGSVSPGSQSVVQAGADEAGRPARKPRRSSRGSDPPATPTTRA |
| 603 | Cas12 | MAIRSIKLKLKTHTGPEAQNLRKGIWRTHRLLNEGVAYYMKMLLLFRQESTGERPKEELQEELICHIREQQQRNQADKNTQALPLDKALEALRQLYELLVPSSVGQSGDAQIISRKFLSPLVDPNSEGGKGTSKAGAKPTWQKKKEANDPTWEQDYEKWKKRREEDPTASVITTLEEYGIRPIFPLYTNTVTDIAWLPLQSNQFVRTWDRDMLQQAIERLLSWESWNKRVQEEYAKLKEKMAQLNEQLEGGQEWISLLEQYEENRERELRENMTAANDKYRITKRQMKGWNELYELWSTFPASASHEQYKEALKRVQQRLRGRFGDAHFFQYLMEEKNRLIWKGNPQRIHYFVARNELTKRLEEAKQSATMTLPNARKHPLWVRFDARGGNLQDYYLTAEADKPRSRRFVTFSQLIWPSESGWMEKKDVEVELALSRQFYQQVKLLKNDKGKQKIEFKDKGSGSTFNGHLGGAKLQLERGDLEKEEKNFEDGEIGSVYLNVVIDFEPLQEVKNGRVQAPYGQVLQLIRRPNEFPKVTTYKSEQLVEWIKASPQHSAGVESLASGFRVMSIDLGLRAAAATSIFSVEESSDKNAADFSYWIEGTPLVAVHQRSYMLRLPGEQVEKQVMEKRDERFQLHQRVKFQIRVLAQIMRMANKQYGDRWDELDSLKQAVEQKKSPLDQTDRTFWEGIVCDLTKVLPRNEADWEQAVVQIHRKAEEYVGKAVQAWRKRFAADERKGIAGLSMWNIEELEGLRKLLISWSRRTRNPQEVNRFERGHTSHQRLLTHIQNVKEDRLKQLSHAIVMTALGYVYDERKQEWCAEYPACQVILFENLSQYRSNLDRSTKENSTLMKWAHRSIPKYVHMQAEPYGIQIGDVRAEYSSRFYAKTGTPGIRCKKVRGQDLQGRRFENLQKRLVNEQFLTEEQVKQLRPGDIVPDDSGELFMTLTDGSGSKEVVFLQADINAAHNLQKRFWQRYNELFKVSCRVIVRDEEEYLVPKTKSVQAKLGKGLFVKKSDTAWKDVYVWDSQAKLKGKTTFTEESESPEQLEDFQEIIEEAEEAKGTYRTLFRDPSGVFFPESVWYPQKDFWGEVKRKLYGKLRERFLTKAR |
| 604 | Cas12 | MVVRSIKLKMKTNSGTDSIYLRKALWRTHRLINEGIVYYMSLLTLYRQDTLGDRTKEEIQSELIKKIREQQRNNGLSEELGSDQEILSLLRHLYELIIPSINGESGDANQLGNKFLYPLVDPNSQSGKGTSNAGRKSKWKRMKEEGNPDWEVEFKKDEERKANDPTIKVFDHLKKYSLLPLFPLFTNNQKDIEWLPMGKRQSVRKWDKDMFIQAIERLLSWESWNRRLGAEREKLEEKIENFYKEHLSGGQIWIEKIREFERVRDRELGETSFSSNDGYLITSRQIRGWDRVYEKWSKISESASKEELWRVVAEQQSKMREGFGDPKVFSFLAELENMDIWRKHPERIYHIATYNGLLKKLSHTKAQATFTLPDAVKHPLWIRYEAQGGTNLNLFKLEETTKKNCKVILSKIIWPTEDGWFEKENVEVDLAPSKQFYRQIKLQDHIKGKQEISFSDYSSGISLKGVLGGSRIQFDRKYIENHQELLPSGDIGPVFFNLVIDLLPIQETRNGRLKSPIGKTLKVVSSEFPKVIDYKPKELTEWINVSSVTGKVGVESITEGMRVMSIDLGQRTSASVSIFEVVKELPKDKEKMLYYNIKDTELFALHKRSFLLNLPGEEVTKRNRQKRKDRRKKLLFIRSQIRMLASVLKLETKNTPDERKKAINKLVEIVDSYEWTESEKEIWNSEFEYLTNKAVFKQEIWRESLIKSHHRMEPHVGQLVSEWRKSLNEGRRNLAGITMWNIEELEDTRRLLISWSKRSRTPGEANRINNDEPFGAKLLEHIQNVKDDRLKQLANLIVMTALGYKYDKEEKSRDKRWKEKYPACQVILFENLNRYLFSLDRSKRENSKLMKWAHRSIPRTVWMQGEMFGLQVGDVRSEYSSRFHAKSGAPGIRCHSLNDEDLKEESFKLKHLIETGFISEEEISSLKKGDIVPWPGGELFVTLSKPYKKGKDIELTVIHADINAAQNLQKRFWQQNSEVYRIPCQLEKAGDDEFFIPKSQTEIVKKYFGKGRFVKINDKKEVYNWEESEKMKIKTESTITLQDLEGFEDIFQTLELAQEQQKKYSTLFRDPSGYFFNEKNWRPQKEFWSIVNNIIRSSLKKKILKNKVEV |
| 605 | Cas12 | MSNPNIPNISPNITLTRDDVVNLLMSSIAMEELGLAHIINAEGEKIQFALGTLQGASGPPATLQQVLEVNQSTQAMLDTIFRQEMMLDSKLKTATNIPTLRGPTGPVGPTGAPGGVISINGQTGVVTLDASNGVMPFMREQSTSSLDDYKDPGIYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGATGATGATGATGPQEPRALREPRAPQARREPRGLLEPQVLRGPQEPRALREPRAPRALREPRALRALRALQELRAPRALRGLQEPRVLQGPRERQVRPEPRVLQGPRERQVRQGLQGLLEPRAKRERQVRPEPREPQERQAPLVQQALLEPQALLGQAL |
| 606 | Cas12 | MNRIYQGRVSKVEIPNPGDKGKPWQPLPNWQDILWQHHELFQDAVNYYLLALLSLARDSANPATPIRKRMDDPASEHQIWTSFRRRGQMRSGMRDSVAKYLCPVKTEPTLDECFAAVLEGNKENPDVLDLALQELLDECDGDGAIQQEGRSMLPRFCSPSYKGDFPQSAAAKDKKSAKEWLPTFLHSAENASNLKLVRQRLKFELFANRDLTGRILDAVESRKRLTEMLGWLTERNPSLAAERERLQKIIAALPDTFNLPAWRGGSVNKEALKQRFYAWLIFEHVESSRITFDILRDSFPTPKEKRKSVSETIQSEKPSLELSDDPIKLARGTRGYVFRAFTSLPCWGADNANLLAWKEFDVAAFKEALKALHQVESKSEERNKERERLLQRHSVMRGSIKWKPSPESEEKEPDVLAGDPRIERLEKLLKTELASEYEMSEGQTVEYGLQPRTIRGFRDLRKEWNKIVKPGEPFTEQKKGNLVTALRSYQIENPNVIGSVRLYEALLEKGNWLVWQEADSATVEKWAKQKFASDPLEALTEERQLLRDIERLKQPIRFTPADAVHSRRQFYLAEKGDLSVKNRFDPQNQTLQVPIAIKNDDHWKQQFVKIHFSAPRAVRDQLIDETMEESKEARWQQPMMEALGLSLKLTKNNAEVSLSECTAVSLMPEELASGDRRILLNFPITLETAPMVKALGKASLWDGQFAAYGDDNFYLRWPKDKWPAEKESNAWYRRLTEFSLLSVDLGQRDAGAFAVIGATTGKPTRPASRFIGEANGQSWHASVRATGVLRLPGEDAHVFRSGQWQEELYGERGRSADATEWTEAKRICETLGLKFDEILGGDPQWFSFPELNDRLLFAVRRAQTRLARLQSWSWMIADETRRENIREAILAADDDELSLKAAAEKSLWPVLAEKLASEVNRLRNLIPQQLVLLANRILPLRGRRWEWIKRDDTSGCHVLRETNPGTDTTSKKVCGQRGLSMKRLEQLDELRRRFQSLNRALMQTPGQTAGLGKSKRGIELPDPCPDLLDKTEQLREQRVNQTAHLILAQALGVRLRTPQKGDALREQNNVHGEYETFRLPVDFIVLEDLSRYLSSQGRGRNENSRLMKWCHRAILLKLKQLCESYGLKILETNAAYSSRFCSRTGVAGFRAVELTPDARKEFRWRKHLNRLEKAASGEIKLDREARAESEHVKRLFDMLDQLNAGRKEAGKPLRTLLAPIAGGQIFIPMQGHATQADINAAINLGLRAIAAPDCHAIHVRIRTERKDKTLRVRTGSNREKVRWEGKNPEIQMNKAADLASLTGDRQPNFFPDPSAIACYDRAKIEGVQLPFASGRGLWGTMNIELQWTRVNQLNNDRAEKKWNLTRSLPEQSSEEDYIPM |
| 607 | Cas12 | MAVKSIKVKLMLGHLPEIREGLWHLHEAVNLGVRYYTEWLALLRQGNLYRRGKDGAQECYMTAEQCRQELLVRLRDRQKRNGHTGDPGTDEELLGVARRLYELLVPQSVGKKGQAQMLASGFLSPLADPKSEGGKGTSKSGRKPAWMGMKEAGDSRWVEAKARYEANKAKDPTKQVIASLEMYGLRPLFDVFTETYKTIRWMPLGKHQGVRAWDRDMFQQSLERLMSWESWNERVGAEFARLVDRRDRFREKHFTGQEHLVALAQRLEQEMKEASPGFESKSSQAHRITKRALRGADGIIDDWLKLSEGEPVDRFDEILRKRQAQNPRRFGSHDLFLKLAEPVFQPLWREDPSFLSRWASYNEVLNKLEDAKQFATFTLPSPCSNPVWARFENAEGTNIFKYDFLFDHFGKGRHGVRFQRMIVMRDGVPTEVEGIVVPIAPSRQLDALAPNDAASPIDVFVGDPAAPGAFRGQFGGAKIQYRRSALVRKGRREEKAYLCGFRLPSQRRTGTPADDAGEVFLNLSLRVESQSEQAGRRNPPYAAVFHISDQTRRVIVRYGEIERYLAEHPDTGIPGSRGLTSGLRVMSVDLGLRTSAAISVFRVAHRDELTPDAHGRQPFFFPIHGMDHLVALHERSHLIRLPGETESKKVRSIREQRLDRLNRLRSQMASLRLLVRTGVLDEQKRDRNWERLQSSMERGGERMPSDWWDLFQAQVRYLAQHRDASGEAWGRMVQAAVRTLWRQLAKQVRDWRKEVRRNADKVKIRGIARDVPGGHSLAQLDYLERQYRFLRSWSAFSVQAGQVVRAERDSRFAVALREHIDNGKKDRLKKLADRILMEALGYVYVTDGRRAGQWQAVYPPCQLVLLEELSEYRFSNDRPPSENSQLMVWSHRGVLEELIHQAQVHDVLVGTIPAAFSSRFDARTGAPGIRCRRVPSIPLKDAPSIPIWLSHYLKQTERDAAALRPGELIPTGDGEFLVTPAGRGASGVRVVHADINAAHNLQRRLWENFDLSDIRVRCDRREGKDGTVVLIPRLTNQRVKERYSGVIFTSEDGVSFTVGDAKTRRRSSASQGEGDDLSDEEQELLAEADDARERSVVLFRDPSGFVNGGRWTAQRAFWGMVHNRIETLLAERFSVSGAAEKVRG |
| 608 | Cas12 | MGLVDTAGGFLIPAALDPAILLSGDGSTNPIRQVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPSRCRCLAFGRSSSAIRRPAASSSEGVKSLRSVFSERSALARSTTSAIVWHTCTGMRSHPSSRAASSRRWPNTSRLSGVTPIGCSKRQGDSPCGCCCGAHLVVAQRCVAFCCHDRGVTEKLLYSSQVVGAAVGAGGVSVPQGVHGRVTG |
| 609 | Cas12 | MRRQHHGGQNARDWRRKVAAAALRQKESVFTYKFGLSVNDGDFDFDAAARTYDITEGIERGSLIGLVCAVHLSGFRLFSKVAETRQFLNRSRYPENEFAQALAAHTEIENPSVTVQSIESVFVTPPRKQDGVARLWSADELAKRLFQTWNNRSPREGERNHPELLLAQGIARAVTKAFSGWKELADNAVHALTCADNYLATLGNRFPKLSDLPPLTAGSTQTGTLAFDPESPFLNMTGNEDIWLHQVVAVCAGRLKRYMPEIDPSSRKFASRLTDSIVSSQNNGLSWLFGNGLRFLRQSSIAQIAETLSVSQNEHRRVEQLKEFADAIPVNPFFATDGYAEFRGSVGGKISSWVSNYWKRICELTVLHSQPPDITIPEGLLASENATLFSGQHTAAAGLVALSARLPSQVRDAGKALFVLSGDGVPRADDIATVEDVAGELAELTGQLAMLDNRIQQEIERAQDANDEGRVGSLASLRPNPTKELKEPPKLNRISGGTADAAGELARLETSLNDLIRARREHFYRLAEWTGNTASLDPLPALAERERKALTDRGMDPTLAEADEYALRRLLHRIAGMARRLSPNEAKRVRETMTPLFLKKREANLYFHNRAGALYRHPFSNSRHQPYSIDLNRARATDWLAWLEERAREMLGLLGSGAPANHEYLRDLLSIETFVFTTRLSGLPAQVPGYLAKPKSDLTNIPPLLAAQLDVDEVSRDVALRAFNLFNSAINGLSFRAFRDSFIVRTKFLRLGHDELFYVPKARAWKPPADYRSAKGKISKGLALPAVKRNEAGSILPRETTQGLSRAKFPEGSHALLSQAPHDWFVELDLRHDKMPQLAGLPVKMNADGLKGWRARRRPTFRLAGPPSFKTWLDRALTSTAVKLGDYTLILDQSFKQSLRVEDGEVRLSAEPAGIKAEIAVPVIDARPFPETEAEALFDNIIGIDLGERRIGYAVFSLPALLKSGNPTRVKPTVVGSVAIPAFRRLMAAVRRHRGSRQPNQKVSQTYSTALQQFRENVVGDVCNRIDTLCERYRAFPVLESSVANFETGANQLKLIYGTVLRRYTFSNVDAHKSARSAYWYSANRWQHPYLFVREWNKAQRTFTGSAKPLAIYPGVTIHPAGTSQICHRCGRNALRALRNMPDRTIRVGKDGLIVLADSTIRLLERADYSDRELKTFKRRKQRPPLNMPVPEGARPRDQLERVLRRNMRQQPQSEMSPDTTQARFTCVYTDCGFEGHADENAAVNIGRRFLERIDIEASSRT |
| 610 | Cas12 | MQEIKRINKIRRRLVKDSNTKKAGKTGPMKTLLVRVMTPDLRERLENLRKKPENIPQPISNTSRANLNKLLTDYTEMKKAILHVYWEEFQKDPVGLMSRVAQPAPKNIDQRKLIPVKDGNERLTSSGFACSQCCQPLYVYKLEQVNDKGKPHTNYFGRCNVSEHERLILLSPHKPEANDELVTYSLGKFGQRALDFYSIHVTRESNHPVKPLEQIGGNSCASGPVGKALSDACMGAVASFLTKYQDIILEHQKVIKKNEKRLANLKDIASANGLAFPKITLPPQPHTKEGIEAYNNVVAQIVIWVNLNLWQKLKIGRDEAKPLQRLKGFPSFPLVERQANEVDWWDMVCNVKKLINEKKEDGKVFWQNLAGYKRQEALLPYLSSEEDRKKGKKFARYQFGDLLLHLEKKHGEDWGKVYDEAWERIDKKVEGLSKHIKLEEERRSEDAQSKAALTDWLRAKASFVIEGLKEADKDEFCRCELKLQKWYGDLRGKPFAIEAENSILDISGFSKQYNCAFIWQKDGVKKLNLYLIINYFKGGKLRFKKIKPEAFEANRFYTVINKKSGEIVPMEVNFNFDDPNLIILPLAFGKRQGREFIWNDLLSLETGSLKLANGRVIEKTLYNRRTRQDEPALFVALTFERREVLDSSNIKPMNLIGIDRGENIPAVIALTDPEGCPLSRFKDSLGNPTHILRIGESYKEKQRTIQAAKEVEQRRAGGYSRKYASKAKNLADDMVRNTARDLLYYAVTQDAMLIFENLSRGFGRQGKRTFMAERQYTRMEDWLTAKLAYEGLPSKTYLSKTLAQYTSKTCSNCGFTITSADYDRVLEKLKKTATGWMTTINGKELKVEGQITYYNRYKRQNVVKDLSVELDRLSEESVNNDISSWTKGRSGEALSLLKKRFSHRPVQEKFVCLNCGFETHADEQAALNIARSWLFLRSQEYKKYQTNKTTGNTDKRAFVETWQSFYRKKLKEVWKPAV |
| 611 | Cas12 | MGKQSTNSSGLKATSGAPSIEKPVTTQRAYTLRLRGIEGDKTWRDSLWATHEAINLGAKAFGDWLLTLRGGLEHTLADATVAGGKGKPARPPSADERRDRRVLLALSWLTVEDERGAPKAPGLIVAYGDDCKSAKDSQDGRDRKVEDALRDILIKRSVAPGAVESWVNDCVVSLKARIRDDAVWVNRSAAFDALRASWRGLTCPNARTVLEQFFGPVADWITLPARAADDGEGDTGGPTAAGSSNDTEFKLVARSFLSENFGTGLKSSKSDISDALMQATRSLAALRGGSPGSAVWSHLCTEFGLLAADDEARAKALRVRIGWTSGRRSKGRLALATAASKASLAQVDIELLIKKLSEEAQEKLAGSTKLPLPWVNDLMAALERSIGFGFVTERNLIDEFGVMLDHAARRVSIACSWIKLAELERRQFEEDAKKLDQVRTQHADAAAFLDTLGCRRGSESGSAGGASVVIRKRAVLGWNEVVGDWARQGCKSVQERIDAARLLQGELEKFGDIRLFEELAANDAQVVWQNAEGETDPTILARYSAGSVAKANQQRFKVPAYRHPDPLRHPVFGDFGKSRWKIDFAVHESDRAQSGGKRVRSLDAAWQSDVRNMRMALWTGKRVEMVPLRWSSKRLSKDLGISDLSLKDPTVVTRGDRLGRAAVGPGDPVRVASVFAEDNWNGRLQAPRVELNRVARLTQSGNLVQARRLRDRLSWLVSFSPKLAPSGPFIDYAAAHGIEPHKKSGEYWPNSAMNKERKGHHSKLIYSRLPSLRVLSVDLGHRFAAACAVWESMSAAQMRLAASKGKVVRGGIAEGALFIHIESTVADGTVRTTVYRRIGEDALPDGSPHPAPWAKLERQFLIKLQGEETPSRMAALDELVMIADWERALGYQPVTASSTKQSNGVAGLMGRTVRLFMHASRRHFDRARIAHNLTAEHRTKPGGVPESLTEETRIELLIDTLALWHGLFAGDRWRDPRAQKEWETSGLPSLKLPGRGDDDETAFGGPGRKAAMVVYRNELKPHAERMAHSDLSNLSKCWTDRWAQDDREWTAKSGRLRSLRRWITPRGLRPVSGDSAQLIERKAAAALRARHVGGLSMQRINTLTDLYRLLKSFKNRPEPSNLRKNIPEKGDARLIGFNQRLLDVRDRLREQRVKQLASRIVEAALGIGRIKIRRVAAGSPRPTLRVDAACHAVVTENLSNYQPAELQTRRENRQLMAWASSKVHKYLAESCQLHGLHLREVQPNYTSRQCSRTGAAGLRGVAVSATDLLTNPWWMRDVAQAKARIEKALKNGRNGAVADQLLVSAEAWALRIPERDRHDRKADESVKAFHAERVILPRKGGDLFVAESARCNGKGHVPALQADLNAAANVGIRALLDPDWPGKWWWVPCVGGTYSPSPEKTSGAKVLEHIDQLPHEMVDPPQVAPKSSAPQPKSSRAAKVKAKEFRAIENRWRTCSSELLSQGKWIPSGEYWSKVEAAVCLVLNDQIT |
| 612 | Cas12 | MAAFQRSYTMNLKPATSEQDKFILWNRLFLTHWSVNEGAKIFGELFLNLRGGLSPELDIFDLDKVKDDKKKKAFVMGRRRLLALGWLSVEDNLSAGEHPFRIREIPVGRNMGKSQASTLLTEILKNKGIKDEAVIKEWIDDCTPSLIANIREDAVWINRASSFNSITPCPTKDEVWIVLSGLLGLRFLDLSLEEVKGKETEYLYDLGEKETQSKSDPSKKARELFGNLFTQNPVLMKNSRDKKDTFAKEFYLAFKEFKDYEKLKEKIESWRKEKEFPLIENPVAEKYPPEVTFTGSPCTVSKRYRKLLVSLELWPSSQDENGNIPKTEKTEDKTHNQVLLDYLLKACNEGNKGTQKIITPVWANNLKAELELKMNEIIRIGESSSTELQRLMIKMAARRISQTLSWIKINEQTKHDAYQKKNKAFKLLSEIDKNGEACKWLENYELFRTDDSGGEEYHISLRAISCWKQILEEWQKNDSPKALREKVKEVQAEEEKFGDARLFEDLADDNARSVWLLPDGNKTPDILNWWCEYRTAEIDESRFKIPCYCHPHPFKHPVYVEYGKSNPKVIFAMKNNKVKKGHIEHGWNPQNPRSIALSLFNNGNRESSLVPFIWESKRLWKDLGGEATQIGDIPRSDRMGLSGKRESVKPKAPFQKEVWNARLQSDRRTLEKLEKYWNPESMKWIDDGKFLIQSKWFITFGPDMETAEGPWKLYLKEKYVDNKILGNRSKENQKRGYRAKKLLSGYPAGMRILSVDLGHRYAASCAVWETITKKQITEELAYQPDNNSLFEHSCKTIDKKIKNTVYRRIGEDSIDAPWAKLEKQFTIKLQGEDKSCYLLRSDEKELFRSILSKLSCLNNDTGHNILEMIENLLRIVKAKIYRQGILARISYSMTAQYKPGKGGQKSPLSDEDKIHYLSENLAAWSAIMGNQEWNEDVISDWYKTYISHLVSGPKPKEGNRKSDRDKIIEYFLPAARKLYDDNETRIKIHDLFKELWDENNKQLSAVLKEIKKIILPKGIRYFDKNTDNPSKWKNNQSKLKQITHRGGLSMQRIVAIEEYYKLAKAYKNHPEPDDLTKNIPLPGDNSSAGFNQRIRDTLERMKEQRVKQIASRIVESALGLGIEGYKKRPLTPENKPCQAIVIEDLSHYRPDELQTRRENRRLMQWSSSKVKKYLKEACEMHDVRLVEISPEYTSRQDSRTGAAGLRCIDINIREFLKDSSRWQNKINTIQKKPANKKSNLDQYLIELNESLGNKYKDKVIPSDNFVRIPRKGGDVFVSSSKESPVSKGIQADLNAAANIGLKALLDPDWAGAWWYILIEVKSNHVIPYGEKYKGSECLRAWKFSGLENQVMKNNMNLWRDLQSQFSGEDKWMSYKEYNELTEKRVINILRERAGLELIKE |
| 613 | Cas12 | MEDYSGFVNIYSIQKTLRFELKPVGKTLEHIEKKGFLKKDKIRAEDYKAVKKIIDKYHRAYIEEVFDSVLHQKKKKDKTRFSTQFIKEIKEFSELYYKTEKNIPDKERLEALSEKLRKMLVGAFKGEFSEEVAEKYKNLFSKELIRNEIEKFCETDEERKQVSNFKSFTTYFTGFHSNRQNIYSDEKKSTAIGYRIIHQNLPKFLDNLKIIESIQRRFKDFPWSDLKKNLKKIDKNIKLTEYFSIDGFVNVLNQKGIDAYNTILGGKSEESGEKIQGLNEYINLYRQKNNIDRKNLPNVKILFKQILGDRETKSFIPEAFPDDQSVLNSITEFAKYLKLDKKKKSIIAELKKFLSSFNRYELDGIYLANDNSLASISTFLFDDWSFIKKSVSFKYDESVGDPKKKIKSPLKYEKEKEKWLKQKYYTISFLNDAIESYSKSQDEKRVKIRLEAYFAEFKSKDDAKKQFDLLERIEEAYAIVEPLLGAEYPRDRNLKADKKEVGKIKDFLDSIKSLQFFLKPLLSAEIFDEKDLGFYNQLEGYYEEIDSIGHLYNKVRNYLTGKIYSKEKFKLNFENSTLLKGWDENREVANLCVIFREDQKYYLGVMDKENNTILSDIPKVKPNELFYEKMVYKLIPTPHMQLPRIIFSSDNLSIYNPSKSILKIREAKSFKEGKNFKLKDCHKFIDFYKESISKNEDWSRFDFKFSKTSSYENISEFYREVERQGYNLDFKKVSKFYIDSLVEDGKLYLFQIYNKDFSIFSKGKPNLHTIYFRSLFSKENLKDVCLKLNGEAEMFFRKKSINYDEKKKREGHHPELFEKLKYPILKDKRYSEDKFQFHLPISLNFKSKERLNFNLKVNEFLKRNKDINIIGIDRGERNLLYLVMINQKGEILKQTLLDSMQSGKGRPEINYKEKLQEKEIERDKARKSWGTVENIKELKEGYLSIVIHQISKLMVENNAIVVLEDLNIGFKRGRQKVERQVYQKFEKMLIDKLNFLVFKENKPTEPGGVLKAYQLTDEFQSFEKLSKQTGFLFYVPSWNTSKIDPRTGFIDFLHPAYENIEKAKQWINKFDSIRFNSKMDWFEFTADTRKFSENLMLGKNRVWVICTTNVERYFTSKTANSSIQYNSIQITEKLKELFVDIPFSNGQDLKPEILRKNDAVFFKSLLFYIKTTLSLRQNNGKKGEEEKDFILSPVVDSKGRFFNSLEASDDEPKDADANGAYHIALKGLMNLLVLNETKEENLSRPKWKIKNKDWLEFVWERNR |
| 614 | Cas12 | MKRIAKFRHDKPVKREAWSKGYRVHKNRIINKVTRSIKYPLVVKDEWKKRLIDDAAHDYRWLVGPINYSDWCRDPNQYSILEFWIDFLCVGGVFQSSHSNICRLAIQLSGGSVFEQEWKDLSPFVRANLIQGIKPAEFIGFLTAEFRSSSNPKNFISKFFEGSNEDLESLTNEFASIVDFIKAKDISLLRKSLPSCKKIAPNLWEKAVGSHSTNELLKLLTKYTRVMLVAEPSHSDRVFSQTVLQSNDQDDPELTGPLPSHKVGKASYLFIPEFIREVNLDKISKLDLSAKSKLAVEQVKKLSELTSDFKQIENQSEAYFGLSTSFNELSNFLGILIRTLRNAPEAILKDQIALCAPLDKDILKITLDWLCDRAQALPENPRFETNWAEYRSYLGGKIKSWFSNYENFFEIPQAASSQQNNNREKKLGNRSAIRALNLKKEAFEKARETFKGDKGTLEKIDLAYRLLGSISPEVLQCDEGLKLYQQFNDELLVLNETINQKFQDAKRDIKAKKEKESFEKLQRNLSSPLPRIPEFFGERAKKGYQKARVSPKLARHLLECLNDWLARFAKVEESAFSEKEFQRILDWLRTSDFLPVFIRKSKDPPSWLRYIARVATGKYYFWVSEYSRKRVQIIDKPIAQNPLKELISWFLLNKDAFSRDNELFKGLSSKMVTLARIMAGILRDRGEGLKELQAMTSKLDNIGLLHPSFSVPVTDSLKDAAFYRAFFSELEGLLNIGRSRLIIERITLQSQQSKNKKTRRPLMPEPFINEDKEVFLAFPKFETKNKVKGTRVVYNSPDEVNWLLSPIRSSKGQLSFMFRCLSEDAKIMTTSGGCSYIVEFKKLLEAQEEVLSIHDCDIIPRAFVSIPFTLERESEETKPDWKPNRFMGVDIGEYAVAYCVIEKGTDSIEILDCGIVRNGAHRVLKEKVDRLKRRQRSMTFGAMDTSIAAARESLVGNYRNRLHAIALKHGAKLVYEYEVSAFESGGNRIKKVYETLKKSDCTGETEADKNARKHIWGETNAVGDQIGAGWTSQTCAKCGRSFGADLKAGNFGVAVPVPEKVEDSKGHYAYHEFPFEDGLKVRGFLKPNKIISDQKELAKAVHAYMRPPLVALGKRKLPKNARYRRGNSSLFRCPFSDCGFTADADIQAAYNIAVKQLYKPKKGYPKERKWQDFVILKPKEPSKLFDKQFYRPN |
| 615 | Cas12 | MAIRSIKLKLKTHTGPEAQNLRKGIWRTHRLLNEGVAYYMKMLLLFRQESTGERPKEELQEELICHIREQQQRNQADKNTQALPLDKALEALRQLYELLVPSSVGQSGDAQIISRKFLSPLVDPNSEGGKGTSKAGAKPTWQKKKEANDPTWEQDYEKWKKRREEDPTASVITTLEEYGIRPIFPLYTNTVTDIAWLPLQSNQFVRTWDRDMLQQAIERLLSWESWNKRVQEEYAKLKEKMAQLNEQLEGGQEWISLLEQYEENRERELRENMTAANDKYRITKRQMKGWNELYELWSTFPASASHEQYKEALKRVQQRLRGRFGDAHFFQYLMEEKNRLIWKGNPQRIHYFVARNELTKRLEEAKQSATMTLPNARKHPLWVRFDARGGNLQDYYLTAEADKPRSRRFVTFSQLIWPSESGWMEKKDVEVELALSRQFYQQVKLLKNDKGKQKIEFKDKGSGSTFNGHLGGAKLQLERGDLEKEEKNFEDGEIGSVYLNVVIDFEPLQEVKNGRVQAPYGQVLQLIRRPNEFPKVTTYKSEQLVEWIKASPQHSAGVESLASGFRVMSIDLGLRAAAATSIFSVEESSDKNAADFSYWIEGTPLVAVHQRSYMLRLPGEQVEKQVMEKRDERFQLHQRVKFQIRVLAQIMRMANKQYGDRWDELDSLKQAVEQKKSPLDQTDRTFWEGIVCDLTKVLPRNEADWEQAVVQIHRKAEEYVGKAVQAWRKRFAADERKGIAGLSMWNIEELEGLRKLLISWSRRTRNPQEVNRFERGHTSHQRLLTHIQNVKEDRLKQLSHAIVMTALGYVYDERKQEWCAEYPACQVILFENLSQYRSNLDRSTKENSTLMKWAHRSIPKYVHMQAEPYGIQIGDVRAEYSSRFYAKTGTPGIRCKKVRGQDLQGRRFENLQKRLVNEQFLTEEQVKQLRPGDIVPDDSGELFMTLTDGSGSKEVVFLQADINAAHNLQKRFWQRYNELFKVSCRVIVRDEEEYLVPKTKSVQAKLGKGLFVKKSDTAWKDVYVWDSQAKLKGKTTFTEESESPEQLEDFQEIIEEAEEAKGTYRTLFRDPSGVFFPESVWYPQKDFWGEVKRKLYGKLRERFLTKAR |
| 616 | Cas12 | MTHAKKIPFPVLKRSTLRKARQRIAAGSITAGERPFNSTVTRVVPVKDPVSDQVWAVAREAAMTLRGFGQGSLFDMLIHLHADGFRLFPSGREREAFFLKDLFDPTEFDDGARRAFGDVMPGFTANSLREILGAPARKCGKVTSVEILLPRLSKGLGVKKSAAPPEVLSSLAAALCEAFPTWSLLTAVDGGVGKVIDDVLRTHGSRLPSLEKAWSTNLPEVPKGLGVPTLAFDDQAPAQSEQTPTGRFAGVVARYLAETFASNPEATAGDASKAVQAKVTTPNGNALSWLFAVGRRAMCSTTLDELAIGLNITSPRGRHALSSLKERMMALPALSVLGERAYPDSRATLQGTVDSLIANYVNRLFELSSSATSIAQTKLILPAAIQGDTAVFDGMPFSAEDVGALFEQLPSEIAKLEHAVKVLVGKERTSTLGYQKAVDDVDEFGVWASSVDAVIGQINARLKTLERAQEPLGKLMGDGKLKRLVNIHEPEGPAVEIIPVLDQELQDVLTSCRTAFADLEARYPMTVAKAQRHAEAEVRNALELASRKEGGLSLASADVPALAKRKILEPIISIARRSSPAMATAVLTECLRQKLIVKGTGSERSLRGYVLSGEQVIYAHPLSRRRSIVRLDREGLQNFDALEFLDALQKDATQRTNVRESLIVEMARQSLLLSALPDRIEIGAISWQTPSQNQHAPWANLRPVNGTVGRSETIKSFTAVFHSRISGLLYRLNRQKFMEKYDLRCFIGSTLLFSPKNADWAPPPQYRHGRFSALLARSDFPWEGAEGTHANAVRLAKFLIDETRNATDLQQAIAAKALLAQLPHDWVVCCDFDGAPSYEGAFVSAGEVSAWAKRSGYLLTPPRHFAGAFLEGFKSTKISPHGLTFERMLERDGDSVIETGRRVTAAFPITQEVAPAAQPWKPRHLAGLDLGEAGLGVCLKNLDNGHEQTLLLKTRKTRLLAHSAEHYRRKDQPRQVFRKQYNQSSENAIKAAIGEVCGLIDNLIARYDAVPVFESQAAAARGSNRMVARVYAGVLQRYTYVVGNGAADATRTSHWLGANRWSYSFGADVIPKVRDLSPEVLRSIKKPENVFRDALGFPGVLANAWRTSMICSVCGTDPIGALEEAIAANQISFVTDNEGEGSLDLGDGRKVTLRVEVPTSSALTKREASRRKRRAPWEAKVGTVWTLTRKSHRDDLLTTIRRSLRRPSSTFQGSTTKQWEFHCPCCGQIQQADVNAASNLVRRYFVRASDNARARQHWADDSKRLAFIASMGPDRSAREEKVS |
| 617 | Cas12 | MNRIYQGRVTKVEKLKNGKSPDDREELKDWQTALWRHHELFQDAVSYYTLALAAMAEGLPDKHPINVLRKRMEEAWEEFPRKTVTPAKNLRDSVRPWLGLSESASFGDALKKILPPAPENKEVRALAVALLAEKARTLKPQKTSASYWGRFCDDLKKKPNWDYSEEELARKTGSGDWVAGLWSEDALNKIDELAKSLKLSSLVKCVPDGQINPEGARNLVKEALDHLEGVSNGTKKEKNDPGPAKKTNNWLRQHASDVRNFIHKNKNQFSSLPNGRLITERARGGGININKTYAGVLFKAFPCPFTFDYVRAAVPEPKVKKVDQEKKSEQSATWTELEKRILRIGDDPIELARKNNKPIFKAFTALEKWSDQNSKSCWSDFDKCAFEEALKTLNQFNQKTEEREKRRSEAEAELKYMMDENPEWKPKKETEGDDVREVPILKGDPRYEKLVKLFGDLDEEGSEHATGKIYGPSRASLRGFGKLRNEWVDLFTKANDNPREQDLQKAVTGFQREHKLDMGYTAFFLKLCERDYWDIWRDDTEVEVKKIREKRWVKSVVYAAADTRELAEELERLQEPVRYTPAEPQFSRRLFMFSDIKGKQGAKHIREGLVEVSLAVKDQSGKYGTCRVRLHYSAPRLIRDHLSDGSSSMWLQPMMAALGLSSDARGCFTRDSKGNVKEPAVALMSDFVGRKRELRMLLNFPVDLDISKLEENIGKKARWEKQMNTAYEKNKLKQRFHLIWPGMELKETQEPGQFWWDNPTIQKEGMYCLAIDLSQRRAADYALLHAGVNRDSKTFVELGQAGGQSWFTKLCAAGSLRLPGEDTEVIREGKRQIELSGKKGRNATQSEYDQAIALAKQLLHNENSAELESAARDWLGDNAKRFSFPEQNDKLIDLYYGALSRYKTWLRWSWRLTEQHKELWDKTLDEIRKVPYFASWGELAGNGTNEATVQQLQKLIADAAVDLRNFLEKALLHIAYRALPLRENTWRWIENGKDGKGKPLHLLVSDGQSPAEIPWLRGQRGLSIARIEQLENFRRAVLSLNRLLRHEIGTKPEFGSSTCGESLPDPCPDLTDKIVRLKEERVNQTAHLIIAQSLGVRLKGHSLFTEEREKADMHGEHEVIPGRSPVDFVVLEDLSRYTTDKSRSRSENSRLMKWCHRKINEKVKLLAEPFGIPVIEVFASYSSKFDARTGAPGFRAVEVTSEDRPFWRKTIEKQSVAREVFDCLDNLVGKGLNGIHLVLPQNGGPLFIAAVKEDQPLPAIRQADINAAVNIGLRAIAGPSCYHAHPKVRLIKGESGTDKGKWLPRKGKEANKRENAQFGNVDLDLEVKFNRLDIDSDVLKGDNTNLFHDPLNIACYGFATIQNLQHPFLAHASAVFSRQKGAVARLQWEVCRAINSRRLEAWQKKAEKAAVKR |
| 618 | Cas12 | MSHELAEQPSPPKNKEPTCEESDAIRKNRRILLALSWLSVEDDCSAPTGTFRVASGKDSEAERKNKVLTAFRSILTARRMRSQDVESWIADCAASLSAKIREDAVWINRSACFDQRALDLKVLSREYAKAAVMSFFGPLDEYFKLPDEADDTKPAVGGDGPDFRTLARQWVSTNFGTGKKSDSEAIAQNLRKLADANLAPFSGKPKAALIAHLSVELDGSTADIDGLCRAIGWNTGRPSKGRVAIERLPDPPTETSIQTMQQKFREEAEAKASSKGLRQVPEWMPAFQKSIERDCGMPFKLGEGRDHIGEFSVMLDHAARRVSIGHSWIKRAEAERRRFEADAQRLNHIPAAAKDWLDQFVQFRSGSSGAAAAGGEYRIRRRAIEGWDEIIKRWKRAACKSPEDRVAAAREVQADPEIEKFGDIQLFEALAADDAECVWRGDGNGTPDPLKDYVAATDALDKMRRFKVPAYRHPDPLAHPVFGDFGNSRGDIRFAVHEAAKATRGTKRIAKDQKEWIRERHGLRMGLWDGQSVRTADLRWSSKRLVDDLALRNHVTTRRTGPVSRADRLGRAAAGLGADEAACVAGLFELPDWNGRLQAPRAQLDAIAACVAANGGKWDDKARKLRDRIEWLVSFSAKLECCGPFMEYASQNGIQPNGKGEYYPHAERNKGRTGHAKLILSRLPGLRVLAVDLGHRFAAACAVWEALSKIAFDAETKGREVVSGGRAADDLYCHTRHLDCAGKARTTIYRRIGPDKLPDGSDHPAPWARLDRQFLIKLQGEERPARAAGPAETAAVQQIETDLGRARGQEDLPPRPVDSLMREAVRTIRIALRRHGDAARIAYAFKPGAKRLKPGGGAQDHTPETHADAILEALLRWHELATGARWRDPWAETQWKDWVQPHISATLPELANDADRWERKRHRAALEQVLRPVAQMLIQRPTDALHQVWSKHWADEDLKWPSRLRWLRNWLLPRGPRARSGAARNVGGLSLLRIATLRELYQTQKAYAMRPEPDDPRKRIAGRNDDRYDELGRSVLQVIERLREQRVKQLASRIVEAALGVGRAKPTRGRQRPQSRVDVPCHAVIIESLRNYRPDELQTRRENRAIMNWSAGKVRKYLEEACQLHGLHLREVMPNYTSREDSRTGLPGVRCVDVPVDPKLGKPKAYWWNSVLSTARKKSIGDAASHDKQGDATSRFIVELAGCLDRLKADGKPLPKTVRVPRIGGDLFVAAPPTSCTAPAHQPHPACDGARALQADLNAAANIGLRALLDPDFPAKWWYVPCIDDQRGLALPRADKVLGSACFPGDPATFGSLLKTRTAAGPAVDGQAAPDRKPRTGTHRPGSAKSRSLGDGKATTNYWSDRSARDLRPADEGGHWQPTNVYWNWVRKRALLGLYSFNGLSPPSDDRP |
| 619 | Cas12 | MAIRSIKLKLKTNTGPEAQNLRKGIWRTHRLLNEGVAYYMNMLLLFRQESTDKKTKQEIHEELIRHIRAQQQRNHADEKTQALPLEKALEALRKLYELLVPSSVGQSGDSQIISRKFLSPLVDPNSEGGKGTSKAGAKPAWQKKKEANDPTWKQDYEKWKKRREEDPTASVITTLEEYGIRPLFPLYTNTVAEIAWLPLKSGQFVRTWDRDMFQQAIEGMLSWESWNRRVQEEYAKLEGKMAQLNEQLEGGEEWIRLLEQYEEKREQELRENMTAANDKFRITKRQMKGWKELYEVWSTFLPSASQEQYKEAIKRVQQRLRGKFGDFHFFQYLSEEENRLIWKGNPQRIHYFVARNELTKKLEKAKQSARRTLPDANKHPLWVRYDARGGNLQDYYLTAESDKPRSRRFVTFSQLIWPSESGWLEKKDVQAELALSRQFYQQVTFLKNDKGKQEIEFKDKGSGTTFSGHLGGAKLQLERSVLENKERKFEEGEIGKAYLNVAIDFKPLQEVKNGRVQAPYGQVLQLIRLPNAFPKVRTYKSEELVEWIKASPQHLSGVESLASGFRVMSIDLGLRAAAATSIFSVEESSDKNATKLAYWIEGTPLVAVHQRSYMLRLPGEQVEQHVWEKRDERGDQHKRVRFQIRRLAEIIRLANKQYGDRWDELNRLDEAVSKEKSPLDQADRTFWEGIVSDLTTALPLNDADWTEAVVQIHRKAELYVGKVVQAWRKRFNADERKGIAGLSMWSIEELDGLRKLLISWSRRTRNPQEVNRFEPEHTGHKRLLTHIQNVKKDRLKQVSHAIVMTALGYIYDEKKQKWCAKYPACQVILFENLSQYRSNLDRSAKENSTLMKWAHRSIPKYVHMQAEPYGIQIGDVRAEYSSRYYAKTGTPGIRCKKLREHDVKGWRLDHLKKRLVNEQFLTEAQVEQLKAGDIIPDDSGELFMTMTDGSGGKEIVFLQADINAAQNLQKRFWQRNSELFKVSCRVIVRDEAEYLVPQAKKVQEKLGKGVFVKKSDTAWKEVYVWDSQAKLKGKTTFTEESESPEQLEDLQEMIEEAEEAKGTYRTLFRDPSGVFFPDFVWNTPKDFWGEVKRKLYGKLRERLLTKVR |
| 620 | Cas12 | MNRIYQGRVTKVEVPDGKDEKGNIKWKKLENWSDILWQHHMLFQDAVNYYTLALAAISGSAVGSDEKSIILREWAVQVQNIWEKAKKKATVFEGPQKRLTSILGLEQNASFDIAAKHILRTSEAKPEQRASALIRLLEEIDKKNHNVVCGERLPFFCPRNIQSKRSPTSKAVSSVQEQKRQEEVRRFHNMQPEEVVKNAVTLDISLFKSSPKIVFLEDPKKARAELLKQFDNACKKHKELVGIKKAFTESIDKHGSSLKVPAPGSKPSGLYPSAIVFKYFPVDITKTVFLKATEKLAMGKDREVTNDPIADARVNDKPHFDYFTNIALIREKEKNRAAWFEFDLAAFIEAIMSPHRFYQDTQKRKEAARKLEEKIKAIEGKGGQFKESDSEDDDVDSLPGFEGDTRIDLLRKLVTDTLGWLGESETPDNNEGKKTEYSISERTLRIFPDIQKQWSELAEKGETTEGKLLEVLKHEQTEHQSDFGSATLYQHLAKPEFHPIWLKSGTEEWHAENPLKAWLNYKELQYELTDKKRPIHFTPAHPVYSPRYFDFPKKSETEEKEVSKNTHSLTTSLASEHIKNSLQFTAGLIRKTNVGKKAIKARFSYSAPRLRRDCLRSENNENLYKAPWLQPMMRALGIDEEKADRQNFANTRITLMAKGLDDIQLGFPVEANSQELQKEVSNGISWKGQFNWGGIASLSALRWPHEKKPKNPPEQPWWGIDSFSCLAVDLGQRYAGAFARLDVSTIEKKGKSRFIGEACDKKWYAKVSRMGLLRLPGEDVKVWRDASKIDKENGFAFRKELFGEKGRSATPLEAEETAELIKLFGANEKDVMPDNWSKELSFPEQNDKLLIVARRAQAAVSRLHRWAWFFDEAKRSDDAIREILESDDTDLKQKVNKNEIEKVKETIISLLKVKQELLPTLLTRLANRVLPLRGRSWEWKKHHQKNDGFILDQTGKAMPNVLIRGQRGLSMDRIEQITELRKRFQALNQSLRRQIGKKAPAKRDDSIPDCCPDLLEKLDHMKEQRVNQTAHMILAEALGLKLAEPPKDKKELNETCDMHGAYAKVDNPVSFIVIEDLSRYRSSQGRSPRENSRLMKWCHRAVRDKLKEMCEVFFPLCERRKAGSAWVSLPPLLETPAAYSSRFCSRSGVAGFRAVEVIPGFELKYPWSWLKDKKDKAGNLAKEALNIRTVSEQLKAFNQDKPEKPRTLLVPIAGGPIFVPISEVGLSSFGLKPQVVQADINAAINLGLRAISDPRIWEIHPRLRTEKRDGRLFAREKRKYGEEKVEVQPSKNEKAKKVKDDRKPNYFADFSGKVDWGFGNIKNESGLTLVSGKALWWTINQLQWERCFDINKRHIEDWSNKQKQ |
| 621 | Cas12 | MGEENNFSQFTGLYELSKTLRFELKPVWETEKLLIENQVFPKDKIVYESYKKIRPYLDKLHLQFIEESLSSVKLDFNEIEKKFLEWDKEKDKTTKNKLKEEIFWKNKKWWLNSNLRKDMVSYFNLNWKKWINNFENKTIVNEKWKEEKIKIKWEWYKILLSDSNLNILSDFFTQEKEWDNVLIEKYIENPNFKENDWEKKFILEKQNLFKSFKWFTTYFTNFNHSRENFYKDDWKSWRIATRIIDENLIFFLKNKKAFDEKYKNNSEITVKFNEKLLNFWEKLEDFFSLDFYNRCYTWKQIEYYNQLIWELNSVVNHEKQAKFSEYTQNKKVSENNKFNKYDFPIFKELYKQILSEKATEQKFIEINDFKELKQNLQELIEKNKQKNKFAINLTKSLIEKIDEYDFEKIYISKLSLNTISSKFFWSNKWFFIQENLEKNIWKKNKNWKIDLPDFIKLSDIKTALENFNKILFEDKENKENIFKEEFDNITENNIFIKFMKIYENEFINLIEWKKNNKWDYEIIWYNKSLLDLEKNILNISDFSDKKEEKEKQIEYIKKYLDSSLNIYRVMKYFALEKGKESVEWEFETDDIFYNEFKKFYIDNEIISYYNEFRNYLTKKPYSSNKLKLNFENWTLIDGWDKNKEPDNYWTILRKNWKYFLALQIKWKNNIFYKKKWSWVIDIEEAYKIDLWDEFYEKMDYKFLPDPKKMLPKVIFAKSNVKLFNPSKEILQIKENETFKTWDKFKVNDFYKIVDFYKENIIKYPDWKIFNFKFSDTKTYNNLSDFYKEIELWSYDLNFRKVSKKYILKSIEEKNIYLFEIYNKDFADWKTWSENLHTMYFKWLFEENNLNNIVLKLNWQAEIFRREASLKEKEVNRAKENKEKSHNIIENARYTKDKLFFHCPIKLNFAKHNEKINQEILKYISDNKEINIIWIDRWEKHLAYYSVINRDWNIIKDKNWNLVKWSLNIVWNNQNYHDKLETREKERQDARWSWKTIWNIKDLKQGYISQVVHKLAELVIEHNAIIVFEDLNSWFKRWRQKIERQVYQKLEKALIEKLNYLTFKDKNFWENWHYLKAYQLTAPFETFEKVWKQTWVIFYTDPSYTSSTCPACWFRKDLYLKYSNLKNALWDIEKIDSIIFDWKRFIFEYNNKKVFTDRDRKRNISSEESWIKWWKTIDNNITEFLEILFKKSNIDYTSWNNLILDIQEINEKELYKWVFDNFNAILNIRNSTIKDNSRTWDFICCPACDFDSRKENKIWIENWDDNWAFNIARKWIIILNKIDSYLNEKWSLDKILWWDMIVKQIDWDDFTHKK |
| 622 | Cas12 | MAQASSTPAVSPRPRPRYREERTLVRKLLPRPGQSKQEFRENVKKLRKAFLQFNADVSGVCQWAIQFRPRYGKPAEPTETFWKFFLEPETSLPPNDSRSPEFRRLQAFEAAAGINGAAALDDPAFTNELRDSILAVASRPKTKEAQRLFSRLKDYQPAHRMILAKVAAEWIESRYRRAHQNWERNYEEWKKEKQEWEQNHPELTPEIREAFNQIFQQLEVKEKRVRICPAARLLQNKDNCQYAGKNKHSVLCNQFNEFKKNHLQGKAIKFFYKDAEKYLRCGLQSLKPNVQGPFREDWNKYLRYMNLKEETLRGKNGGRLPHCKNLGQECEFNPHTALCKQYQQQLSSRPDLVQHDELYRKWRREYWREPRKPVFRYPSVKRHSIAKIFGENYFQADFKNSVVGLRLDSMPAGQYLEFAFAPWPRNYRPQPGETEISSVHLHFVGTRPRIGFRFRVPHKRSRFDCTQEELDELRSRTFPRKAQDQKFLEAARKRLLETFPGNAEQELRLLAVDLGTDSARAAFFIGKTFQQAFPLKIVKIEKLYEQWPNQKQAGDRRDASSKQPRPGLSRDHVGRHLQKMRAQASEIAQKRQELTGTPAPETTTDQAAKKATLQPFDLRGLTVHTARMIRDWARLNARQIIQLAEENQVDLIVLESLRGFRPPGYENLDQEKKRRVAFFAHGRIRRKVTEKAVERGMRVVTVPYLASSKVCAECRKKQKDNKQWEKNKKRGLFKCEGCGSQAQVDENAARVLGRVFWGEIELPTAIP |
| 623 | Cas12 | MGEENNFSQFTGLYELSKTLRFELKPVWETEKLLIENQVFPKDKIVYESYKKIRPYLDKLHLQFIEESLSSVKLDFNEIEKKFLEWDKEKDKTTKNKLKEEIFWKNKKWWLNSNLRKDMVSYFNLNWKKWINNFENKTIVNEKWKEEKIKIKWEWYKILLSDSNLNILSDFFTQEKEWDNVLIEKYIENPNFKENDWEKKFILEKQNLFKSFKWFTTYFTNFNHSRENFYKDDWKSWRIATRIIDENLIFFLKNKKAFDEKYKNNSEITVKFNEKLLNFWEKLEDFFSLDFYNRCYTWKQIEYYNQLIWELNSVVNHEKQAKFSEYTQNKKVSENNKFNKYDFPIFKELYKQILSEKATEQKFIEINDFKELKQNLQELIEKNKQKNKFAINLTKSLIEKIDEYDFEKIYISKLSLNTISSKFFWSNKWFFIQENLEKNIWKKNKNWKIDLPDFIKLSDIKTALENFNKILFEDKENKENIFKEEFDNITENNIFIKFMKIYENEFINLIEWKKNNKWDYEIIWYNKSLLDLEKNILNISDFSDKKEEKEKQIEYIKKYLDSSLNIYRVMKYFALEKGKESVEWEFETDDIFYNEFKKFYIDNEIISYYNEFRNYLTKKPYSSNKLKLNFENWTLIDGWDKNKEPDNYWTILRKNWKYFLALQIKWKNNIFYKKKWSWVIDIEEAYKIDLWDEFYEKMDYKFLPDPKKMLPKVIFAKSNVKLFNPSKEILQIKENETFKTWDKFKVNDFYKIVDFYKENIIKYPDWKIFNFKFSDTKTYNNLSDFYKEIELWSYDLNFRKVSKKYILKSIEEKNIYLFEIYNKDFADWKTWSENLHTMYFKWLFEENNLNNIVLKLNWQAEIFRREASLKEKEVNRAKENKEKSHNIIENARYTKDKLFFHCPIKLNFAKHNEKINQEILKYISDNKEINIIWIDRWEKHLAYYSVINRDWNIIKDKNWNLVKWSLNIVWNNQNYHDKLETREKERQDARWSWKTIWNIKDLKQGYISQVVHKLAELVIEHNAIIVFEDLNSWFKRWRQKIERQVYQKLEKALIEKLNYLTFKDKNFWENWHYLKAYQLTAPFETFEKVWKQTWVIFYTDPSYTSSTCPACWFRKDLYLKYSNLKNALWDIEKIDSIIFDWKRFIFEYNNKKVFTDRDRKRNISSEESWIKWWKTIDNNITEFLEILFKKSNIDYTSWNNLILDIQEINEKELYKWVFDNFNAILNIRNSTIKDNSRTWDFICCPACDFDSRKENKIWIENWDDNWAFNIARKWIIILNKIDSYLNEKWSLDKILWWDMIVKQIDWDDFTHKK |
| 624 | Cas12 | MATAINYPTTQRAYTLRLRGIDPQDQSWRDALWATHEAVNRGAKVFGEWLLTLRGGLDHQLADAPVKVRGGTTRLPSDEERRDRRVLLALSWLSVEDAHGAPPDASLIVAKGTDSADCRARKLADALIAILQARSVAASEIGDPSKPPEDQPGTWLGDCMGSLSAAIRDDAVWVNRSKAFDAATQSCPSLTRDEIWDFLEPFFASPDAYLKPERAESDEGDSTSAATEDKAKDLVQKAGGWLSKRMGAGGGANFQDLARAYQAIAQWASSAQPGQSAQQAVGSLAGYLSQHGFSPTANDATGVLAVISGPGYKSATRNHITAIATSPEITPQDLSKLQELATKDKAGCSSKIGGKGPRPYATMILQQVEAACGFTYLQSDGPARHREFSVMLDHAARRVNVAHSWIKNAEAERRQFESDARRIKKVPQDALNWLRGYCEERGGASGSLEGYRIRRRAIDGWDQVVIRWSRSDCQSADDRIAAARQLQDDPEIDKFGDIQLFEALAAEEALCVWKPDGNPTAQPLKDFVAATEADAKKKRFKVPAYRHPDPLRHPVFTDFGNSRWGIEYSAHRAPAKCDELGQQVDRLTQAVAEAQRNLDGATAAQRASRESKLAEAQSKLVAAQTEFAAINDPHRVELKLWNGQAVAAIPMRWSSKRLIADLSLRRATQPSSDQRIGVTRADRLGRAAGNADDGRPVTITGLFQQDHWNGRLQAPRAQLDAIAKHVDKHGWDAKARRQIARIRWVVSFSAELSQQGPWFEFCHRFGEDAPARPFVSRHGEYAVKHRDNDQRKGHAKLILSRLPGLRVLAVDLGHRYAAACAVWEAISSDQMRQACAAANAPAPHPLAMYIHLKSTTAKGKPTTTIYRRIGPDKLPDGTPHPAPWARLDRQFLIKLPGEDRPARAASPDEIKAVEDFEDSVGRVRTAVDPPRKRGVDLLMHDAVRTARLALARHGRRARIAFQLISQVRILPGGRPQTLDDAGRRDLLNDTLADWYALATDSRWTDAAARQLWNERLAALNGGFTIDPPADASQPEAERTRAQRRQAEQELRHRLASLVEALFCNPTLCQQLHQAWTDRWNADDQQWRSRLKWLSRWLLPRGGSRRDGSRRHVGGLSLTRISTLIDFRRKVQVGYFTRLRPDGSRAEIGPQFGQSTLDAIQRLKDQRIKQLTSRIVEAALGIGVEQDRIWDAAKRKWRTVKRPREPRYHVDDQGVQQRDPRFQACHAVVIEDLSHYRPEETRTRRENRATMDWKSAETRKRLADHCQLYGLHLRDVNPQYTSRQDSRTGAPGCRCVDVSVADFLTKPAWRKQVAQARGKVASNRGDARDRLLVELDHQLTTANSLRGEMDSLRIPVNGGEVFVSADPRSPLAAGIQADLNAAANIGLRALMDPDFLGTWWYVPCDPSTKKPHIEKVKGSILATVGALQATSEEAAAPPRRGRGGTRSAAPREVINLWRDPSAVRIQDATAGEVWDVTPVYWSIVKDRVVDVLRQRNTKSGD |
| 625 | Cas12 | MAVRSVKLKLLVPRDGSAESVRKRKALWATHQFVNDAAAAYAELLLEMRQEDVCRGTDDHGKDVIEPAAHWQAKLRARLAAKQLPPVAVAEALPLLKAFYGSRLIKSFVANDKGVAGTGNATDLNTWLSGLVDPASVAGEKTELRKQLLAELPLCEAADADFEGAARKMLAKSDAREALLEGPGTGVGWPAAYNANPTDSVWLDMLHKAAAKARLELADTTVSELKKLGVFPLLQAASSNRVFGSGVLNPFERMAAAQAAAALLPWETKRHEMRKRRDKFADQLNQWDTEFGASHATALAAIRAFEAEESERARRESLGNEGTGYRIGGRELRDAWTLLRDWLKGHSTATAAAREDKVRELQAKQGRSFGSHRLLSWLAKPAQQWLADHSAGDVVTRIAVRNARQRKLDTARTLPIWTGADAVKHPRFANFDPPNNTNQPGFDLRAGTQKGRLTLRLSLLTERADGLLLAQDHDFQLVPSRQMAEIVLHKDGKERALSWQSQDGIGRQVGDVGGSALLFSRDHAECLLERKQITRLERGAWPAALPVWFKLSLDIGAEHKALLKQRFKWGVWLNSALVTRNAKDAKGVPPPVGTRVLAVDLGLRSAATVSVWQVVDAATPVVAGKWRVPLSDTLSAVHERSAMLALPGEHVDAGVLAARRAANEKLAGLLAATSHLSTVFKLGRAEQGDRRRELLERLGEGDDRRARAAVATTAAERDGLRAVLGATQDAWAGAVAAVWRRLETDLAGAIAAYRKQQREDVQLRREARHGPGASQLPKQAAAERLLGGKSAWQIEYKERVRKLLTRWIMRQRPGDTAVRRLARKDLGKYCGGLLDHLTALKEDRAKTTADLIVQAARGRVRAHKDAHGRQQDRELWLAKYAPCDLIVMEDLGRYRFATDRPPSENRQLMQWTHREVFRLVQMQAEVEGIQVLETGAEFSSKFDARTWAPGVRCEPITKLWVERYRNGEMPWLADKADEWRREGIELAQLVPGQLLPTGSGEQFVAVSATGGLRVRHADLNAAQCIALRALTGHGTAFRLTARRLGDVFVSAKGLGKRPQGALWREFGSALPPAVVVLRPAGEVRYALRPFASARDAAAALGLQLGALRNVDATDAESDAEDGDLAELLAGADPDRATFFRDPSGDVHGGAWVQAKVFWAEVRRHVRLGLQAQGLLPAAARSSEPRQMQLPLAGALPGDDIPL |
| 626 | Cas12 | MAAFQRSYTMNLKPATSEQDKFILWNRLFLTHWSVNEGAKIFGELFLNLRGGLSPELGIFDLNKDKDDRKKKALVMGRRRLLALGWLSVEDNLSAGDHPFRIREIPVGRNMEIKQATTLLTEILKNKGVKDEAVIKEWIDDCTPSLIANIREDAVWINRAKSFYSMNPCPTKDEVWKILSYVLNTSFLDLSLNDSSERDNTKNKKGTKENEKDVSNKSKELYGWLFTKNPNKMREAGENKDKFINNFRENFNTFTDYANLKVEIELWRKNNISNTLLITQKAKYPPEVKEANHPSKFSVGYRKLLVHLELWPSSKDENGDIPKGIEGKDKSHNQILLDYLLEVCNEGNKTTKKVIVPAWADGIKTELESKASIKVGDSTSSVLQRLMIKMAARRISQTLSWIKINEQVRHDAYQKKNKAFKLLCEIDKNGEACKWLENYELFRRDDSGGEEYHISARAISCWKQILEEWQKNDSSKALREKVKVVQAAEDKFGDARLFEDLADDNARSVWLLPDGNKTPDILNWWCEYRTADIDESRFKIPCYCHPHPFKHPVYVEYGKSNPQVIFSLKHDKARKNRIDNGWNPKNPRILALLLLDIVRQKSTLAPFVWESKRLWKDLGGDATVTYKIPRSDRMGLSSIGNIDYARPEVPFLKEKWNARLQSDRRTLEKLEKYWNPESMKWIDDGKFLIQSKWFITFGPDMETAEGPWKLYLKENINDNNYLGNRSKENQKRGYRAKKLLSGYPAGMRILSVDLGHRYAASCAIWETITKKQITEELAYQPDKNSVFEHSCKTIDKKIKNTVYRRIGDDSIDAPWAKLEKQFTIKLQGEDKSCYLLRSDEKELFRSILSKLSCLNNDTGHNILEMIENLLRIVKAKIYRQGILARISYSMTAQYKPGKGGQKSPLSDEDKIHYLSENLAAWSALMGNQEWNEDGISDWYKKYISHLVSGPKPKEGNRKSDRDKIIEYFLPAARKLYDDNETRINIHDLFKELWDENNKQLSAVLKEIKKIILPKGIRYFDKNNDSSSRWKNNQSKLKQITHRGGLSLRRIVAIEGYYKLAKAYKNHPEPDNLTKNIPLPGDNSSAGFNQRIRDTLERMKEQRVKQIASRIVESALGLGIEGYKKRPLTPESKPCQAIVIEDLSHYRPDELQTRRENRRLMQWSSSKVKKYLSEACEMHDVLLVEISPEYTSRQDSRTGVAGLRCIDINIREFLKDSSSWQNKIKTIQMKPTNKKSNLDQYLIELNESLGERYKDKVIPSDKFVRIPRKGGDIFVSSSKESPVSKGIQADLNAAANIGLKALLDPDWAGAWWYILIEAKSNHVIPYGKKYKGAECLRDFKFSGLENQVMKNNMNLWRDLQSQFSSEDKWMSYKEYNELTEKRVINILRERAGLELIEE |
| `627 | Cas12 | MPTRTINLKMVLGRKDDTAELRRALWTTHEHVNLAVAEVERVLLRCRGRSYWTLDRRGDPVHVPESQVAEDALAMAREAQRRNGWPVVGEDEEILLALRYLYEQIVPSCLLDDLGKPLKGDAQKIGTNYAGPLFDSDTCRRDEGKDVACCGPFHEVAGKYLGALPEWATPISKQEFDGKDASHLRFKATGGDDAFFRVSIEKANAWYEDPANQDALKNKAYNKDDWKKEKDKGISSWAVKYIQKQLQLGQDPRTEVRRKLWLELGLLPLFIPVFDKTMVGNLWNRLAVRLALAHLLSWESWNHRAVQDQALARAKRDELAALFLGMEDGFAGLREYELRRNESIKQHAFEPVDRPYVVSGRALRSWTRVREEWLRHGDTQESRKNICNRLQDRLRGKFGDPDVFHWLAEDGQEALWKERDCVTSFSLLNDADGLLEKRKGYALMTFADARLHPRWAMYEAPGGSNLRTYQIRKTENGLWADVVLLSPRNESAAVEEKTFNVRLAPSGQLSNVSFDQIQKGSKMVGRCRYQSANQQFEGLLGGAEILFDRKRIANEQHGATDLASKPGHVWFKLTLDVRPQAPQGWLDGKGRPALPPEAKHFKTALSNKSKFADQVRPGLRVLSVDLGVRSFAACSVFELVRGGPDQGTYFPAADGRTVDDPEKLWAKHERSFKITLPGENPSRKEEIARRAAMEELRSLNGDIRRLKAILRLSVLQEDDPRTEHLRLFMEAIVDDPAKSALNAELFKGFGDDRFRSTPDLWKQHCHFFHDKAEKVVAERFSRWRTETRPKSSSWQDWRERRGYAGGKSYWAVTYLEAVRGLILRWNMRGRTYGEVNRQDKKQFGTVASALLHHINQLKEDRIKTGADMIIQAARGFVPRKNGAGWVQVHEPCRLILFEDLARYRFRTDRSRRENSRLMRWSHREIVNEVGMQGELYGLHVDTTEAGFSSRYLASSGAPGVRCRHLVEEDFHDGLPGMHLVGELDWLLPKDKDRTANEARRLLGGMVRPGMLVPWDGGELFATLNAASQLHVIHADINAAQNLQRRFWGRCGEAIRIVCNQLSVDGSTRYEMAKAPKARLLGALQQLKNGDAPFHLTSIPNSQKPENSYVMTPTNAGKKYRAGPGEKSSGEEDELALDIVEQAEELAQGRKTFFRDPSGVFFAPDRWLPSEIYWSRIRRRIWQVTLERNSSGRQERAEMDEMPY |
| 628 | Cas12 | MVTRALNLKLVVPRRPGELTKAEALWSTHDIVNRATSYYESQLLLCRQQDYQTRELTVSAGDQAPDLDALIANARDRNRYRGLEKPQVVREKLRNLYEAIVPPAIGKTGTAQAVGAFVSPLLDADSRGFTEIFDKIEALPNWVDGVRAEEPDALEAAADWLKSPQGKERLRPTGAPPTWIKLAKKKDAGWAAAFVADIDKKLKEVEGTPTLMQELRALGVMPLFPSFFASRIAGHKGAVSTWDRLALRLAVAHLLSWESWVELAAKEHAARVAKLEKFRDDNILGEIADAVEALRLYEKERTEELQQKAQLDAEEVRTTSRTIRGWVDLREKWLKTDASPDALISLVAAEQKRKSGKFGDPQLFRWLAKPENHFVWNKPDFDPPSLFASLRMIEGLVERSKETAWMTLPDARLHPRSSQWEPHGGGNLKTFRLEQGEGGSLSVTLPLLRKSGDDSYVEEEHAFSLAGSKQIPNASLDVRRNKYCLSYRTPTGEEAEAVVGSADLLLDWYFLQQRSEHRPEEGDIGPAFLKLALDITPIDPVWGEREKTPAIHHFKTASGKNTRHADGVAPGFRMLAVDLGIRTLATCSVFELKATAPAGRLSFPIAHLDLHAVHERSFTLTLDGEDPDRDAERWRENKSAELRRLRMGLTRYRNIRNMREDAPDEREVLLEDLQEKVQEHGWAFEEPLLRELAKHKDTPEPIWEAELTKALAQFRSDFGVIVGEWRRSNRARSTDSHAGKSMWAIDHLTNSRRFLMSWSLLSKPGQIRRLDRDKQGVFAKHLLDHLEGLKADRLKTGSDLIVQAARGFRRDKRGNWHKAYKPCHGILFEDLSRYRMRTDRPRRENSQLMKWAHRAVPKEVGMQAEVYGIRVEDTGAAFSSRFHAASHTPGIRMHPICQKDLENEWLLDEIEKQNSGVKRRELKLGQLVQLNGGELFACVTASGVKTLHADINAAQNLQRRFFTRHGDAFRIVARKVLVDEEEVWVPRSLGKRLLGALGSHGKLVPTGHESGSCRFEEITTRAWSKLSGEKLSDDRVGNEEDQIIASIEEEALERTGEVVVFFRDPSGQVLPRDLWYPSKTFWSIVKSTTLSKLKAAP |
| 629 | Cas12 | MEDKQFLERYKEFIGLNSLSKTLRNSLIPVGSTLKHIQEYGILEEDSLRAQKREELKGIMDDYYRNYIEMHLRDVHDIDWNELFEALTEVKKNQTDDAKKCLEKIQEKKRKEIYQYLSDDAVFSEMFKEKMISGILPDFIRCNEEYSEEEKEEKLKTVALFHRFTSSFNDFFLNRKNVFTKEAIATAIGYRVVHENAEIFLENMVAFQNIQKSAESQISIIERKNEHYFMEWKLSHIFTADYYMMLMTQKAIEHYNEMCGVVNQHMKEYCQKEKKNWNLYRMKRLHKQILSNASTSFKIPEKYENDAEVYESVNSFLQNVMEKTVMERIAVLKNNTDNFDLSKIYITAPYYEKISNYLCGSWNTIADCLTHYYEQQIAGKGARKDQKVKAAVKADKWKSLSEIEQLLKEYARAEEVKRKPEEYIAEIENIVSLKEVHLLEYHPEVNLIENEKYATEIKDVLDNYMELFHWMKWFYIEEAVEKEVNFYGELDDLYEEIRDIVPLYNKVRNYVTQKPYSDTKIKLNFGTPTLANGWSKSKEYDYNAILLQKDGKYYMGIFNPVQKPEKEIIEGHSHPLEGNEYKKMVYYYLPSANKMLPKVLLSKKGMEIYQPSEYIINGYKERRHIKSEEKFDLQFCHDLIDYFKSGIERNPDWKVFGFHFSDTDTYQDISGFYREVEDQGYKIDWTYIKEADIDRLNEEGKLYLFQIYNKDFSEKSTGRENLHTMYLKNLFSEENIREQVLKLNGEAEIFFRKSSVKKPIIHKKGTMLVNRTYMEEMHGESVKKNIPEKEYQEIYNYMNHRWKGELSAEAKEYLKKAVCHETKKDIVKDYRYSVDKFFIHLPITINYRASGKEALNSVAQRYIAHQNDMHVIGIDRGERNLIYVSVINMQGEIIEQKSFNVVNKYNYKEKLKEREQNRDEARKNWKEIGQIKDLKEGYLSGVIHEIAKMMIKYHAIVAMEDLNYGFKRGRFKVERQVYQKFENMLIQKLNYLVFKDRSADEDGGVLRGYQLAYIPDSVKKLGRQCGMIFYVPAAFTSKIDPATGFVDIFNHKAYTTDQAKREFILSFDEICYDVERQLFRFTFDYANFATHNVTLARNNWTIYTNGTRTQKEFVNRRVRDKKEVFDPTEKMLKLLELEGVEYQSGANLLPKLEKISDPHLFHELQRIVRFTVQLRNSKNEENDVDYDHVISPVLNEEGKFFDSSKYENKEEKKESLLPVDADANGAYCIALKGLYIMQAIQKNWSEEKALSPDVLRLNNNDWFDYIQNKRYR |
| 630 | Cas12 | MAQASSTPAVSPRPRPRYREERTLVRKLLPRPGQSKQEFRENVKKLRKAFLQFNADVSGVCQWAIQFRPRYGKPAEPTETFWKFFLEPETSLPPNDSRSPEFRRLQAFEAAAGINGAAALDDPAFTNELRDSILAVASRPKTKEAQRLFSRLKDYQPAHRMILAKVAAEWIESRYRRAHQNWERNYEEWKKEKQEWEQNHPELTPEIREAFNQIFQQLEVKEKRVRICPAARLLQNKDNCQYAGKNKHSVLCNQFNEFKKNHLQGKAIKFFYKDAEKYLRCGLQSLKPNVQGPFREDWNKYLRYMNLKEETLRGKNGGRLPHCKNLGQECEFNPHTALCKQYQQQLSSRPDLVQHDELYRKWRREYWREPRKPVFRYPSVKRHSIAKIFGENYFQADFKNSVVGLRLDSMPAGQYLEFAFAPWPRNYRPQPGETEISSVHLHFVGTRPRIGFRFRVPHKRSRFDCTQEELDELRSRTFPRKAQDQKFLEAARKRLLETFPGNAEQELRLLAVDLGTDSARAAFFIGKTFQQAFPLKIVKIEKLYEQWPNQKQAGDRRDASSKQPRPGLSRDHVGRHLQKMRAQASEIAQKRQELTGTPAPETTTDQAAKKATLQPFDLRGLTVHTARMIRDWARLNARQIIQLAEENQVDLIVLESLRGFRPPGYENLDQEKKRRVAFFAHGRIRRKVTEKAVERGMRVVTVPYLASSKVCAECRKKQKDNKQWEKNKKRGLFKCEGCGSQAQVDENAARVLGRVFWGEIELPTAIP |
| 631 | Cas12 | MAVKSIKVKLRLSECPDILAGMWQLHRATNAGVRYYTEWVSLMRQEILYSRGPDGGQQCYMTAEDCQRELLRRLRNRQLHNGRQDQPGTDADLLAISRRLYEILVLQSIGKRGDAQQIASSFLSPLVDPNSKGGRGEAKSGRKPAWQKMRDQGDPRWVAAREKYEQRKAVDPSKEILNSLDALGLRPLFAVFTETYRSGVDWKPLGKSQGVRTWDRDMFQQALERLMSWESWNRRVGEEYARLFQQKMKFEQEHFAEQSHLVKLARALEADMRAASQGFEAKRGTAHQITRRALRGADRVFEIWKSIPEEALFSQYDEVIRQVQAEKRRDFGSHDLFAKLAEPKYQPLWRADETFLTRYALYNGVLRDLEKARQFATFTLPDACVNPIWTRFESSQGSNLHKYEFLFDHLGPGRHAVRFQRLLVVESEGAKERDSVVVPVAPSGQLDKLVLREEEKSSVALHLHDTARPDGFMAEWAGAKLQYERSTLARKARRDKQGMRSWRRQPSMLMSAAQMLEDAKQAGDVYLNISVRVKSPSEVRGQRRPPYAALFRIDDKQRRVTVNYNKLSAYLEEHPDKQIPGAPGLLSGLRVMSVDLGLRTSASISVFRVAKKEEVEALGDGRPPHYYPIHGTDDLVAVHERSHLIQMPGETETKQLRKLREERQAVLRPLFAQLALLRLLVRCGAADERIRTRSWQRLTKQGREFTKRLTPSWREALELELTRLEAYCGRVPDDEWSRIVDRTVIALWRRMGKQVRDWRKQVKSGAKVKVKGYQLDVVGGNSLAQIDYLEQQYKFLRRWSFFARASGLVVRADRESHFAVALRQHIENAKRDRLKKLADRILMEALGYVYEASGPREGQWTAQHPPCQLIILEELSAYRFSDDRPPSENSKLMAWGHRGILEELVNQAQVHDVLVGTVYAAFSSRFDARTGAPGVRCRRVPARFVGATVDDSLPLWLTEFLDKHRLDKNLLRPDDVIPTGEGEFLVSPCGEEAARVRQVHADINAAQNLQRRLWQNFDITELRLRCDVKMGGEGTVLVPRVNNARAKQLFGKKVLVSQDGVTFFERSQTGGKPHSEKQTDLTDKELELIAEADEARAKSVVLFRDPSGHIGKGHWIRQREFWSLVKQRIESHTAERIRVRGVGSSLD |
| 632 | Cas12 | MSNPNIPNISPNITLTRDDVVNLLMSSIAMEELGLAHIINAEGEKIQFALGTLQGASGPPATLQQVLEVNQSTQAMLDTIFRQEMMLDSKLKTATNIPTLRGPTGPVGPTGAPGGVISINGQTGVVTLDASNGVMPFMREQSTSSLDDYKDPGIYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGATGATGATGATGPQEPRALREPRAPQARREPRGLLEPQVLRGPQEPRALREPRAPRALREPRALRALRALQELRAPRALRGLQEPRVLQGPRERQVRPEPRVLQGPRERQVRQGLQGLLEPRAKRERQVRPEPREPQERQAPLVQQALLEPQALLGQAL |
| 633 | Cas12 | MIKKSNFWQFTGLYELSKTLRFELKPVWETLNILEKDWVSQKDKEVEENYNKIKVFFDSLHREFVKQSLENWYLELLENFYNSYIELNKNIENKKNKSLQKLFEKSSKELKKELVSFFEWKWNDWKQKYSFLKKWWIDVLNEKEVLDLMWEFYPKEKELFKKFDKFFTYFSNFKESRKNFYADDWRAWAIATRVIDENLITFIKNIEDFKKFKNNFSDFIENGFSDWKIKINWFTLEEKQVFDLDFYNNCLLQDWIDNYNKILWWFSEENWNKIQWINEKINLFKQNQNKTNSKDVKFPRFKLLYKQILSEKEKLIFVDEIENDEKLINFIKESKNNNLIKVEKAIEITENFIKNNETFELDKIYLSKISINTISNKFFSSWDYILKEGFDKWEIKEFISFEDLKNAFGKIKYENLEDIFKSNYITDYIAINWEDLYKNFLNIFLYEFKQNINEINFYNSELEKLFLEKFEKTETQVQIIKNYFDSVLSLYKMTKYFALEKGKKKIEDLETDNNFYNDFFVYYEDFEIWKDYNLVRNFITKKQVKTDKFKLNFENSQFLTGWDKDKEKERLWIILKKDEKYYLWILKNNKIFNSYNYESWDFYEKMSYKQLNNVYRQLPRFAFSKAKREVYWITPELEQIKEEFDIFQKNKEKWEKFDIEKLKKLINCYKKWFIKTYENEFDLEKIKNTDYLDLATFYDEIEQKTYKIDFNKISENFINAKVNSWELYLFQIYNKDFSETKKAWSKENIHTKYFKLLFDEKNLEKLVIKLSWWAEMFFREKTEKLKTKLDKSWKEVLEHRRYSKDKIMLHLSITLNANKWDSFWFNKMVNEYLNKNEDIKIIWIDRGEKHLAYYSVIDKNWKIEEIDTLNIIKSSDWKITNYLEKLEKIESSRKDSRVSWWEIENIKELKNGYISQVVNKLAELIIKYNAIIVFEDLNIWFKRWRQKIEKQIYQKLELALAKKLNYLTQKDKNDNEVLWNLKALQLVPKVNDYQDIANYKQSGIMFYTRANYTSTTCPCCWFRKNIYISNSDTKEKQKKDFEKIDIKFDGEKFIFSYEIIQDKKAKQKSNKTNFSVNSNFSRFKYNSKKMLVEEVNLNLELQNLFKDIDLKWDINKQILEKDSYFYKSLTYYFNLILQLRNSDSKNDIDYITCPSCNYHSKDWFQGLEYNADANWAYNIARKWIIMLDRIEKNFEKPDLYVSDIDWDNFTQK |
| 634 | Cas12 | MIKKSNFWQFTGLYELSKTLRFELKPVWETLNILEKDWVSQKDKEVEENYNKIKVFFDSLHREFVKQSLENWYLELLENFYNSYIELNKNIENKKNKSLQKLFEKSSKELKKELVSFFEWKWNDWKQKYSFLKKWWIDVLNEKEVLDLMWEFYPKEKELFKKFDKFFTYFSNFKESRKNFYADDWRAWAIATRVIDENLITFIKNIEDFKKFKNNFSDFIENGFSDWKIKINWFTLEEKQVFDLDFYNNCLLQDWIDNYNKILWWFSEENWNKIQWINEKINLFKQNQNKTNSKDVKFPRFKLLYKQILSEKEKLIFVDEIENDEKLINFIKESKNNNLIKVEKAIEITENFIKNNETFELDKIYLSKISINTISNKFFSSWDYILKEGFDKWEIKEFISFEDLKNAFGKIKYENLEDIFKSNYITDYIAINWEDLYKNFLNIFLYEFKQNINEINFYNSELEKLFLEKFEKTETQVQIIKNYFDSVLSLYKMTKYFALEKGKKKIEDLETDNNFYNDFFVYYEDFEIWKDYNLVRNFITKKQVKTDKFKLNFENSQFLTGWDKDKEKERLWIILKKDEKYYLWILKNNKIFNSYNYESWDFYEKMSYKQLNNVYRQLPRFAFSKAKREVYWITPELEQIKEEFDIFQKNKEKWEKFDIEKLKKLINCYKKWFIKTYENEFDLEKIKNTDYLDLATFYDEIEQKTYKIDFNKISENFINAKVNSWELYLFQIYNKDFSETKKAWSKENIHTKYFKLLFDEKNLEKLVIKLSWWAEMFFREKTEKLKTKLDKSWKEVLEHRRYSKDKIMLHLSITLNANKWDSFWFNKMVNEYLNKNEDIKIIWIDRGEKHLAYYSVIDKNWKIEEIDTLNIIKSSDWKITNYLEKLEKIESSRKDSRVSWWEIENIKELKNGYISQVVNKLAELIIKYNAIIVFEDLNIWFKRWRQKIEKQIYQKLELALAKKLNYLTQKDKNDNEVLWNLKALQLVPKVNDYQDIANYKQSGIMFYTRANYTSTTCPCCWFRKNIYISNSDTKEKQKKDFEKIDIKFDGEKFIFSYEIIQDKKAKQKSNKTNFSVNSNFSRFKYNSKKMLVEEVNLNLELQNLFKDIDLKWDINKQILEKDSYFYKSLTYYFNLILQLRNSDSKNDIDYITCPSCNYHSKDWFQGLEYNADANWAYNIARKWIIMLDRIEKNFEKPDLYVSDIDWDNFTQK |
| 635 | Cas12 | MATRSFILKIEPNEEVKKGLWKTHEVLNHGIAYYMNILKLIRQEAIYEHHEQDPKNPKKVSKAEIQAELWDFVLKMQKCNSFTHEVDKDVVFNILRELYEELVPSSVEKKGEANQLSNKFLYPLVDPNSQSGKGTASSGRKPRWYNLKIAGDPSWEEEKKKWEEDKKKDPLAKILGKLAEYGLIPLFIPFTDSNEPIVKEIKWMEKSRNQSVRRLDKDMFIQALERFLSWESWNLKVKEEYEKVEKEHKTLEERIKEDIQAFKSLEQYEKERQEQLLRDTLNTNEYRLSKRGLRGWREIIQKWLKMDENEPSEKYLEVFKDYQRKHPREAGDYSVYEFLSKKENHFIWRNHPEYPYLYATFCEIDKKKKDAKQQATFTLADPINHPLWVRFEERSGSNLNKYRILTEQLHTEKLKKKLTVQLDRLIYPTESGGWEEKGKVDIVLLPSRQFYNQIFLDIEEKGKHAFTYKDESIKFPLKGTLGGARVQFDRDHLRRYPHKVESGNVGRIYFNMTVNIEPTESPVSKSLKIHRDDFPKFVNFKPKELTEWIKDSKGKKLKSGIESLEIGLRVMSIDLGQRQAAAASIFEVVDQKPDIEGKLFFPIKGTELYAVHRASFNIKLPGETLVKSREVLRKAREDNLKLMNQKLNFLRNVLHFQQFEDITEREKRVTKWISRQENSDVPLVYQDELIQIRELMYKPYKDWVAFLKQLHKRLEVEIGKEVKHWRKSLSDGRKGLYGISLKNIDEIDRTRKFLLRWSLRPTEPGEVRRLEPGQRFAIDQLNHLNALKEDRLKKMANTIIMHALGYCYDVRKKKWQAKNPACQIILFEDLSNYNPYEERSRFENSKLMKWSRREIPRQVALQGEIYGLQVGEVGAQFSSRFHAKTGSPGIRCSVVTKEKLQDNRFFKNLQREGRLTLDKIAVLKEGDLYPDKGGEKFISLSKDRKLVTTHADINAAQNLQKRFWTRTHGFYKVYCKAYQVDGQTVYIPESKDQKQKIIEEFGEGYFILKDGVYEWGNAGKLKIKKGSSKQSSSELVDSDILKDSFDLASELKGEKLMLYRDPSGNVFPSDKWMAAGVFFGKLERILISKLTNQYSISTIEDDSSKQSM |
| 636 | Cas12 | MAESMYNQQNINQKSTETPTEEDALGIKNPLLTPTTLGQNFLSLQSISPLGSRSINLINWSLISPNQTLLQNFQDNENWQDSSISEFRPFSKNSLTENSPIIEPQSDKTLPSSVPIQLSSELPIQQPPETSFIDSESPIPQPPETPSSDSESPIGQTENIFPFQIDQKTRRNSVIQNSKSSISHFFQKKSEFPREKISQENVNKLTKKSAENQDISTTEESVINSIETNDLQTINPTDIPNPEIPVSTLQKQPDSTIDNIPQTESVSTINPTDIPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSKNNRIPQLITFLKLNQYQQLILQIFLTQKFSFQLSKNNRIPPLITFLKLNQYQQLIPRVLLHQKFLFQLFKNNRIPPLITFPKLNQYQQLIPRIFLTQKFPFQISRNNRIPQLITFPKLNQYQ |
| 637 | Cas13 | MGAIKNKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSKIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQDKEEAIFLPRGLFNEAIINCLKKSKLKQLIESPTREKSPALNVSYLIQNYFRTYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIGSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDLSNDSSA |
| 638 | Cas13 | MARDNFLNTIKLLADKMKLGAISGFGKDGNEVNDINKLFGDKNTYANIENVVEFYFPWIKALEGRFSLDKGDRNLNDMKMFYKSVLTAFFTAVDSLRNKYTHYSHKDLNIREIKIECTLGGKDYCIGLLNALDCIYDSAVNLLKLRFMVGEDEVAHLRRCKAVNKKVVVRTEKDGFYYRLSDNGGVTEKGVIFIASMFLNRKYGFLFLKQLEGFKRSDEKRYRLTLEAFLAFSNIKPVDRLKSDKLDRASLGLDMLNELTKIPKELSETLSVDCLYKYLASDGEDDLRSRIRYQDRFVPLALEFISQSDEFKDFRFYTYVGNYVYKGYIKRLIDGTDKERYLSDRLCGFYKSVNDASSDAIAQKYGVEIKDSNEPDYMLPDSFRPHVLRATPHFVINNNNIGIKICGNDCLPIVNGKGVESPEPDYWLSIYELPAMLFYAYLREKNGKRFKDYKSIRELIEGVEKKADEKNDRDKGALMARHIDKEIIWTQTKLDEVKRLEEKKVAAYGKKGRVVLKSGRMADLLAHDMVRLQPATKGSDKITGVNFQALQVSLAYFKRDILADVFSRAMLTTGNHRHPFLYRIDVSHCSSLRDFYVAYLGERRKYFEDVAKKIAKNKLNTPCHILRRLQREGSGEEAGKDVKPKFLPRGIFTDSIKNCLEQSKLNIYIRNARNDVKPAINAAYLILMYYKEIEKGEFQGFYGEKRRYDILEEGKPLDLAERKKALASIKPAKIDVSEANMPMSKEEHLMRKRYHAVCNNESAIRMYQVQDILLLLMAKDIFKKALSEGVMSKKIGLENLNGIFDAPVNFVKNFDNIKLTATGIKIKNYGKVCRLGTDFKFNSLIKAFHKVYSKSVEMDYSDYLKEEEEFEKYRLNMVKLCREVERGITEDLHLSLDGKSHLSFNDDVIKPYNDKYNVFNGDDLTFFINARNMFMHGDYKYECVKYVVSEHFKGSLNDVSFAKETYGHFCNLLESMRKKTGLRIDI |
| 639 | Cas13 | MKKKISLKEQRNTKKAENKLKYQKAQAERAAAAQQTAAGAESEENPCFDVVKDTKRKALNPLHVEIEAPSAKKSSVKANGLKSLLLTDGKTVMTSFGRGSEANVEKRFDETGTKTFDRDPELFSAKPLETGYRIQRFNASPKDAGLAYRPAGVRPDQIGAKAALEKRYFGKETPGDNIHVQIAYQIQDIEKLLAVYISNIIYAVNNVTGVSAMKDSKGRPVDLLGDYGILGEEGLTKRLQRIPEQADEEAKALQAFLCSERLSYFGKEFCLVRNSPKQPDKEEKRQYKLMRVLCLLGELRQFLVHGKKKEKEFAWLYRLDRQLSQEYRKLLGEFYDAQVDKVNKSFLTNSTVNLEVLFRALKTGTDPERKTVTQEYYQFTVRKEDGNLGFSLKTLREILLSAYKHEVRDKEYDSIRHKLYQLFSFALYHYYKTGVGAERREAFVAKLRAVMTAEAKQRAYADEAAEIWNDEGSGIRAAFLEILEAVDFGSAVKGIKARSSVAGDKRFAEWLEEVRIRPEGVSCFTKLMYLLTRFLDGKEINELLTGLINKLENIQSFLDVMQQEHAETGLSDAFSFFEYSGEIAAELRMTRSFARMAAADPEAKRFMVVDGAKLLGFNPKDTESEDEGIIRAIYGDACAEYLQFSEEEKEAFYVQEGLYGKEREKFSPYAYFHTDTSLRNFIAKNVVESARFRYVIRYVSPEIARKYARQEALVRFALHRVPLLQLRRYYQSCCGPKKDPDAAECVDFLAGVVNRVDFANFTDVRTGDSSKSEQEKKQKYQAIVGLYLTVVYWIVKNLVNVNSRYVMAFHILERDTVLLEGKRLFVGGMKAEDPFLLTDGYVSRQDAYVRKRIGENKRANRHGLNCVLENRNALGSDPASTDAAASLIWSYRNAAAHLTAVAAAQEYVSELREIHSYFEVYHYAMQRYLKSGAEFAELVSKNGPASGKIAAWANAVDRCHSFCKDWLWLLNVPFAYNPARYKNLSIANLFDKNEAAPVTEDASEQKEDE |
| 640 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSKIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKHLIESPTREKSPALNVSYLIQNYFRAYFEDQSQEFYAQPRNYRLFDNLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDFSNDSSA |
| 641 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPRGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRNYTLVNNNGLSEKGYAFFISKFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSRIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKHLIESPTREKSPALNVSYLIHNYFRAYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDLSNDSSA |
| 642 | Cas13 | CILDFFTQDKAIAEYQLGVEFLQKNLPVIRYLYLPTSHKRFENVPKNQLISEQRNYFKNSLKVLKNLIRDYRNFYTHHFHKPIPVFPETYKLLDDLFLAVANDVKKHRMKTDASKQLLKKGLIEELAQLEKLKLEDLKKLKREGKKVNLNDKEAITNAILNDSFSHLLPKENTISKYYSAVPTEDIDTENGVTISESGIIFLLGLFLTKKQSEDLRSRVKGFKAKLIVNPENPINKKNNSLKYMATHWVFGYLGFKGLKNRFTTTFTKDTLLAQIVDELSKVPDELYQVLPEELKNEFLEDMNEYLKEENSESLDKATVIHPVIRKRYENKFAYFALRFLDEFVDFPTLRFQLHLGNYVHDKREKPIEGTKYVTERIVKEKIKAFAKLSEAAQLKQKYFEEKENHQSIGLQLYPNPSYNFVGNNIPIHLNLNEHFFPKEVKIVAGRLKKRNSSYKSDHPEEYKVRTDNKIKPDAILQDLGKPEKLAPVAMLSLNELPALLHLVLTKKTPEEIEIIIAQKIAERYNVLTNYKAGDDISKGQITKNLLKAKQKKEVNLDKLQLAIEKEIAVTNDKLQTIALHIKERNDPKQKRKYVFTNKEIGLQVTWLANDLKRFMPKGSRQNWRGQHHSQLQKSLAFYDIQPKEPLSLLEEVWDFKNEAYLWNNGIRRSFDKRDFISFYTSYLNNRKETFQRFKDQLNGIRSNKKILDKFIKQQHLWNLFHKRLYVIDTIEEQVEKLLVKPMQFPKGVFDHKPTYIKGKSIQENPECFADWYVAWNQHTDYQKFYSWDRDYKSAYLSGEQEKTEKRFIRVQGSKINKVKQQDVLLAKMASIIFNELYLPEDAEHLDLNLSDIYKTQTERKAEIEAALIQSHKTTGDNSANIIKSTSAWTLTVPYCSKNIYEPQVKLKELGKFKKFIASQKVQTLFEYKPQKIWNKTELEEVLELKANSYEVIRRDYLLKSIQEFEKYMIKKLPTLIDTNEHPNFNKYLTTFLKSLELVSEEDAKWLISKKDFDTTPIDELKKQSKIMEKAFLLVMIRNKFSHNQLPRKIYYDEIYKNVPNAVSINFNELFLEYTNQTILEFK |
| 643 | Cas13 | NRRDMEASPGRLARYIVLKTLYERAFPRWLEAREAETLNGWIGRAADRATVAARHINKDENAAARMAGLVRLADGKGIADFTDRLAAETASEYRVQRGYDSDPAVARKQAKHIEDLRCDVVGQAFEAYLADVARKLAWTMNDPPGGPLPDEKKASLETAAPPAEDAGAEAEEDWLARLYFLLHMLPVEEVGGLRHQLRKWSVLEREPDSVLEREPDADVEAIERILGLYLDMHDAKFEGGDGVAGAEALTDLFTSPAQFRRVCPEDGAGNGGHVPWRGLREMLRFGGGEPRLMWAFRKWPIGDGMVDGLNALEATVAEAHKQREDLHAKWAGKKGFSRKDKDEYRAALETVVVHRHLAAHVQLVNHARLHRLAMAVLARLADYAGLWERDLYFTTLALIRLENGKPEDVFRSRELEWLREGRIVDALKELKKNADGSEPAVGTALQRLFGKGILTGGGTVSVRRDLLHFNMLQRKANEPFNLTTAVNDTRKLMAYDRKLKNAVSRSIIELLAREGLDLAWEMKDHQLAGAVLKTRQAVHLGGAKVGGGPITEDLHGPEFTAMAAALFGGEARAATDAAEAATGRPDRRERKSGGASPRPARPAKTLPAPTPRLLPAAGERVEGVLVEEKTRKGGWKAAVEIGGPRIVGDIFNSGEVPSDAEPGLEAEFVVRVANPANASFMWLSPDVEERLKKAAAPRRGGRPAGRQR |
| 644 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSKIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKHLIESPTREKSPALNVSYLIQNYFRAYFEDQSQEFYAQPRNYRLFDNLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDFSNDSSA |
| 645 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPRGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRNYTLVNNNGLSEKGYAFFISKFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSRIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKHLIESPTREKSPALNVSYLIHNYFRAYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDLSNDSSA |
| 646 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSKIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKHLIESPTREKSPALNVSYLIQNYFRAYFEDQSQEFYAQPRNYRLFDNLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDFSNDSSA |
| 647 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPRGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRNYTLVNNNGLSEKGYAFFISKFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSRIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKHLIESPTREKSPALNVSYLIHNYFRAYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDLSNDSSA |
| 648 | Cas13 | MEEANRYIYGAYFNMARDNFLNTIKLLADKMKLGATSGFGKDGNEVNDINELFGDKNTYANIENVVEFYFPWIKALEGRFSLDKGDRNLNDMKMFYKSVLTAFFTAVDSLRNKYTHYSHKDLNIREIKIECTLGGKDYCIGLLNALDCIYDSAVNLLKLRFMAGEYEVAHLRRCKAVNKKVVVRTEKDGFYYRLSDNGGVTEKGVIFIASMFLNRKYGFLFLKQLEGFKRSDEKRYRLTLETFLAFSNIKPVDRLKSDKLDRASLGLDMLNELTKIPRELSETLSVDCLYKYLTSDGEDDLRSRIRYQDRFVPLALEFISQSDEFKDFRFYTYVGNYVYKGYIKRLIDGTDKERYLSDRLCGFYKSVNDASSDAIAQKYGVEIKDSNEPDYMLPDSFRPHVLRATPHFVINNNNIGIKICGNDCLPIVNGKGVESPEPDYWLSIYELPAMLFYAYLREKNGKLLKDYKSIRELIEDVEKKADEKNDRDKGALMARHIDKEIIWTQTKLDEVKRLEEKKVAAYGKKGRVVLKSGRMADLLAHDMVRLQPATKGSDKITGANFQALQVSLAYFKRDILADVFSRAMLTTGNHRHPFLYRIDVSHCSSLRDFYVAYLGERRKYFEDVAKKIAKNKLNTPCHILRRLQREGSGEEAGKDVKPKFLPRGIFTGSIKSCLEKSALNINIRNARNDVKPAINAAYLILMYYKEIEKGEFQGFYGEKRRYDILEEGKPLDLDERKKALASIKPAKIDVSEANMPMSKEEHLMRKRYHAVCNNESAIRMYQVQDILLLLMAKDIFKKALSEGVMSKKIGLENLNGIFDAPVNFVKNFDNIKLTATGIKIKDYGKVCRLGTDFKFNSLVKAFHKVYSKSVEMDYSDYLKEEEEFEKYRLNMVKLCREVERGITEDLHLSLDGKSHLGFNDDVIKPYNDKYNVFNGGDLTFFINARNMFMHGDYKYECVKYVVSEHFKGSLNDVSFAKETYGHFCNLLESMRKKTGLRIDI |
| 649 | Cas13 | MGAIKNKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSKIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQDKEEAIFLPRGLFNEAIINCLKKSKLKQLIESPTREKSPALNVSYLIQNYFRTYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIGSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDLSNDSSA |
| 650 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSKIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKHLIESPTREKSPALNVSYLIQNYFRAYFEDQSQEFYAQPRNYRLFDNLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDFSNDSSA |
| 651 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEIEHLRRYTRKKGRVVLKTEDDHFYYTLVNNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSRIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQDKEEAIFLPRGLFNEAIINCLKKSKLKQLIESPTREKSPALNVSYLIQNYFRTYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDLSNDSSA |
| 652 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSKIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKHLIESPTREKSPALNVSYLIQNYFRAYFEDQSQEFYAQPRNYRLFDNLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDFSNDSSA |
| 653 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSKIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFETFALHFLDKQPDFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSMPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAEMLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKQLIESPTREKSPALNVSYLIQNYFRAYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDLSNDSSA |
| 654 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPRGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRNYTLVNNNGLSEKGYAFFISKFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSRIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKHLIESPTREKSPALNVSYLIHNYFRAYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDLSNDSSA |
| 655 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEIEHLRRYTRKKGRVVLKTEDDHFYYTLVNNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSRIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQDKEEAIFLPRGLFNEAIINCLKKSKLKQLIESPTREKSPALNVSYLIQNYFRTYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDLSNDSSA |
| 656 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSRIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKQLIESPTREKSPALNVSYLILNYFRTYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRINSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDFSNDSSA |
| 657 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSKIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKHLIESPTREKSPALNVSYLIQNYFRAYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDLSNDSSA |
| 658 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSKKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSRIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKQLIESPTREKSPALNVSYLILNYFRTYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRINSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDFSNDSSA |
| 659 | Cas13 | MKHVFAAYFSDARLNIMASLNDVREKSGLKRYKNEAENVQNFEGIFPKNIASDIRDKRITLLRIRFPFIETIVDPGRGKENPASKYGELSWLFQLVNDMRNVFIHSTGSEEEIDYQHHKKIFNALRKVYDCGLRTVKSRFQLENDTTMPLLRCDNRGRPKPFNKFSLALCTSPERNGNKKQSDVLHDFGRVLLCSLFLEKRQISGLVSHFWDKNGYGTDWNDSEQTIIRELLYVNRIRLPSQRLRTDSTLTSVTLDTISELARCPRPLFELLDADGQENFRVGRSPKNPQNRNDDPSYLLLRGHQSRFIPLAMRHLDFDSKCKLRFAVDLGQYYHSVRLKPAESFIDGNPGIRRLGQKIIAFGRLKDFEDAEKPEIWKKLEENGQKFAEEEEELLKRASITGSPEELKPYIIKTFPHYHLYRDKIGFCIDWEEKKDQKVKYPNLGVRGEDSKSTDSGGIKKRENRQLSRHQFWISPNKIIDLAFYHYLQTEIKQEITASVKTGRKKCPYPSVENILKEYYEGMVVLIKELKEQGPLPPWTEIPQISERRAECHRWINKEIIKAENFTISLADLPKAIRRHLKVLDSRETSVSDIIKRTKTMIEETCKKQKEIEYLLKYPKKRGKKGFRPIKHGNIADFLTEDLLRFQPKDSSKKNGGKLTSKNYQILQKAIAYYDKPYCIVDLLKKSGLLEGEFKHPFLCKIITEENHEQYSTLIDFYQKYLEERKAFLEGFIDTFVAGSSIPGWLRLRKPSTFESWLDQQLDEDEKLCQPLPVPKSLFYQMLLKMTAEKLDLTPEMLCKKGTQRFIRDSQEVEVKPSVSWFIRQYMEHRDDTAQEMYMFKRRHELFDFFHDKKKTTKKLHEEKTCCLSEKVRQEELESVHKGIEKLKKLLRTYKRSEKKIRHFSTMDMVLYLLAKKNFEKLILCDETSGPDWSLKTLETKILSTKIKYELNVPGTDRTIVHRACAIKKTGELRLLVRDRRLPSLLDYYPKTEKSIDQEEIKVELADYSCKRIEAMKLISDLEQKIEESCNRGFCKPVPDKLKKIFGAKKHGTLLYALYHRFHEEKDKSVDEQGFNKDCFAHARQIRNAFAHNEYPVSEQFNQIVRQVNETSQPHNHNSPVNHPKIADQLVKELDKLYKPWRNFLKRIAG |
| 660 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPRGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRNYTLVNNNGLSEKGYAFFISKFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSRIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKHLIESPTREKSPALNVSYLIHNYFRAYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDLSNDSSA |
| 661 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPRGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRNYTLVNNNGLSEKGYAFFISKFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSRIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKHLIESPTREKSPALNVSYLIHNYFRAYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDLSNDSSA |
| 662 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSKIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKHLIESPTREKSPALNVSYLIQNYFRAYFEDQSQEFYAQPRNYRLFDNLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDFSNDSSA |
| 663 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPRGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRNYTLVNNNGLSEKGYAFFISKFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSRIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKHLIESPTREKSPALNVSYLIHNYFRAYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDLSNDSSA |
| 664 | Cas13 | MGAIKNKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSKIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQDKEEAIFLPRGLFNEAIINCLKKSKLKQLIESPTREKSPALNVSYLIQNYFRTYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIGSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDLSNDSSA |
| 665 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSKKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSRIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKQLIESPTREKSPALNVSYLILNYFRTYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRINSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDFSNDSSA |
| 666 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMKHLRNYTLVNNNGLSEKGYAFFISKFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSKIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKQLIESPTREKSPALNVSYLIQNYFRTYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRINSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDLVYAKKIIAEFKMKIENIKLEDLSNDSSA |
| 667 | Cas13 | MGAIKNKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSKIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQDKEEAIFLPRGLFNEAIINCLKKSKLKQLIESPTREKSPALNVSYLIQNYFRTYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIGSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDLSNDSSA |
| 668 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSRIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKQLIESPTREKSPALNVSYLILNYFRTYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRINSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDFSNDSSA |
| 669 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSKIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKHLIESPTREKSPALNVSYLIQNYFRAYFEDQSQEFYAQPRNYRLFDNLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDFSNDSSA |
| 670 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPRGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRNYTLVNNNGLSEKGYAFFISKFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSRIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKHLIESPTREKSPALNVSYLIHNYFRAYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDLSNDSSA |
| 671 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSRIPIELYQTLEPKYREMYNATLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKQLIESPTREKSPALNVSYLILNYFRTYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRINSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDFSNDSSA |
| 672 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSKIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFETFALHFLDKQPDFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSMPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAEMLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKQLIESPTREKSPALNVSYLIQNYFRAYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDLSNDSSA |
| 673 | Cas13 | MGAIKNKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSKIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQDKEEAIFLPRGLFNEAIINCLKKSKLKQLIESPTREKSPALNVSYLIQNYFRTYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIGSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDLSNDSSA |
| 674 | Cas13 | MGKLYGYKRWYEIEQNNGEKGSFKRKIRVKRVYDEESKSYVVRRDEVEDTELIKNKNFIINLKDYKTRNSNIQKFYDKFHVGNILFKLKASVKKGKKTFYKDLEKAETVLTNVEILEQTIKLANNSKINRKKLEKELTQLNINEKSEEVTIDNIKIYLRQGEKFDKKENINAIKWRKLTSEELTIKVEIYRECQSINSNLYSLLDYVLSNEEYDDRYYLEEVENKLLIFGTGNKNKNGRNYYYFDYVLKSLSKIKGLIKKDDRNLNYLMFLFNIQKTSENKEHFINKIFNYFKYEVRIEKEDIVEFLVGELEYYDLIKRIEKKPSENQNQTNLEKTYILLDKHEKLKETIDTKNEIVQKLTIELKNNNLRNRIEIILHKYKILELVDKLNKNIKNGKINTELYGIYKEHYGQCIEYINFNELALEEKELYKIIYRYLKGRIEKLLQNRNKIKIGELRIEDIFIFQKLLEKIEIRVKQYLLEHILYLGKLKHHNIDEVNTIRFVEEHANEELSLELITLFSATNVELNRLLKVKGENGSYEKDYDFFSAKPDKGKIKIKDTNSVMKFELLKKLKFINSEATETNQEAIDFLKEAYNLRNNILHGKNEEIIEDNKKNLSKSYKNINELIEELRPSDNEICKSLNLDIIFKGNRKINDINAKLFGNNREKIYLPSFSKLVPEIKNIIESYDKNGTFNDERIKKIVLNGAIYVNKILYLKECSNKDGEFIKNLKEELNKDSKEKSKYVSIEELYKKSQISASKGNKKAIYKYQRKIIEIYLKYLKENYVEILDFSKLNLNIEQIENDIKNRKNSENKVLIESIKQKVFPENDFEYIISIFALLNDNIFINKIRNRFFSTDTWLKNNRYSNIIKILDEVISVNLLRTELLNTSIDIEEIKDDVLTEDIENIIPEIREEILQRTKKDFKTLLGNNAISKEGLSNEDIDKIRNIEDAKINLDFVNNEIKVSLKEYGSLPPNGVLSRNTSKYYNNEIAKKIDQISILTFTKKSIGTISDEKFRNIYWQERKESDESKKIFVYNKNILYLVTKHSFEKLYKNFLEEELNNLKLEDTKYLRDLDLRREKNLKVDNTLKEINEKVRGYSKEYKKKFIENLKNNDEYFGKVVSGRFKNYQEFKEIYDEVSEYKKIRDVVNFNPLNKVYNYLIEINWKLAIQMARAERDLHYIVNGLNELKLIELNQGQNDGISRAYPKYKLNKEKNKKELRLEECHYNFDIDNYKKFEKICEKLGIDLSENGELQQENETNIRNYISHFYILRKPFVDISISEAIKRVSKLLSYRTRYNNSTYSSVFEVFKKDVELNYDFLKKKIELNGKTYDEVVQRKKISCLELESYLDYKPIIKKILF |
| 675 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSKIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKHLIESPTREKSPALNVSYLIQNYFRAYFEDQSQEFYAQPRNYRLFDNLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDFSNDSSA |
| 676 | Cas13 | MGAIKNKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSKIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQDKEEAIFLPRGLFNEAIINCLKKSKLKQLIESPTREKSPALNVSYLIQNYFRTYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIGSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDLSNDSSA |
| 677 | Cas13 | MGAIKNKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSKIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQDKEEAIFLPRGLFNEAIINCLKKSKLKQLIESPTREKSPALNVSYLIQNYFRTYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIGSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDLSNDSSA |
| 678 | Cas13 | MGKLYGYKRWYEIEQNNGEKGSFKRKIRVKRVYDEESKSYVVRRDEVEDTELIKNKNFIINLKDYKTRNSNIQKFYDKFHVGNILFKLKASVKKGKKTFYKDLEKAETVLTNVEILEQTIKLANNSKINRKKLEKELTQLNINEKSEEVTIDNIKIYLRQGEKFDKKENINAIKWRKLTSEELTIKVEIYRECQSINSNLYSLLDYVLSNEEYDDRYYLEEVENKLLIFGTGNKNKNGRNYYYFDYVLKSLSKIKGLIKKDDRNLNYLMFLFNIQKTSENKEHFINKIFNYFKYEVRIEKEDIVEFLVGELEYYDLIKRIEKKPSENQNQTNLEKTYILLDKHEKLKETIDTKNEIVQKLTIELKNNNLRNRIEIILHKYKILELVDKLNKNIKNGKINTELYGIYKEHYGQCIEYINFNELALEEKELYKIIYRYLKGRIEKLLQNRNKIKIGELRIEDIFIFQKLLEKIEIRVKQYLLEHILYLGKLKHHNIDEVNTIRFVEEHANEELSLELITLFSATNVELNRLLKVKGENGSYEKDYDFFSAKPDKGKIKIKDTNSVMKFELLKKLKFINSEATETNQEAIDFLKEAYNLRNNILHGKNEEIIEDNKKNLSKSYKNINELIEELRPSDNEICKSLNLDIIFKGNRKINDINAKLFGNNREKIYLPSFSKLVPEIKNIIESYDKNGTFNDERIKKIVLNGAIYVNKILYLKECSNKDGEFIKNLKEELNKDSKEKSKYVSIEELYKKSQISASKGNKKAIYKYQRKIIEIYLKYLKENYVEILDFSKLNLNIEQIENDIKNRKNSENKVLIESIKQKVFPENDFEYIISIFALLNDNIFINKIRNRFFSTDTWLKNNRYSNIIKILDEVISVNLLRTELLNTSIDIEEIKDDVLTEDIENIIPEIREEILQRTKKDFKTLLGNNAISKEGLSNEDIDKIRNIEDAKINLDFVNNEIKVSLKEYGSLPPNGVLSRNTSKYYNNEIAKKIDQISILTFTKKSIGTISDEKFRNIYWQERKESDESKKIFVYNKNILYLVTKHSFEKLYKNFLEEELNNLKLEDTKYLRDLDLRREKNLKVDNTLKEINEKVRGYSKEYKKKFIENLKNNDEYFGKVVSGRFKNYQEFKEIYDEVSEYKKIRDVVNFNPLNKVYNYLIEINWKLAIQMARAERDLHYIVNGLNELKLIELNQGQNDGISRAYPKYKLNKEKNKKELRLEECHYNFDIDNYKKFEKICEKLGIDLSENGELQQENETNIRNYISHFYILRKPFVDISISEAIKRVSKLLSYRTRYNNSTYSSVFEVFKKDVELNYDFLKKKIELNGKTYDEVVQRKKISCLELESYLDYKPIIKKILF |
| 679 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEIEHLRRYTRKKGRVVLKTEDDHFYYTLVNNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSRIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQDKEEAIFLPRGLFNEAIINCLKKSKLKQLIESPTREKSPALNVSYLIQNYFRTYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFRIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDLSNDSSA |
| 680 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSKIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKHLIESPTREKSPALNVSYLIQNYFRAYFEDQSQEFYAQPRNYRLFDNLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDFSNDSSA |
| 681 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYIRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSRIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKQLIESPTREKSPVLNVSYLIQNYFRAYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDFSNDSSA |
| 682 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMKHLRNYTLVNNNGLSEKGYAFFISKFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSKIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKQLIESPTREKSPALNVSYLIQNYFRTYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRINSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDLVYAKKIIAEFKMKIENIKLEDLSNDSSA |
| 683 | Cas13 | MGAIKNKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSKIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQDKEEAIFLPRGLFNEAIINCLKKSKLKQLIESPTREKSPALNVSYLIQNYFRTYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIGSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDLSNDSSA |
| 684 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSRIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKQLIESPTREKSPALNVSYLILNYFRTYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRINSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDFSNDSSA |
| 685 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSKIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKHLIESPTREKSPALNVSYLIQNYFRAYFEDQSQEFYAQPRNYRLFDNLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDFSNDSSA |
| 686 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSKIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKHLIESPTREKSPALNVSYLIQNYFRAYFEDQSQEFYAQPRNYRLFDNLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDFSNDSSA |
| 687 | Cas13 | MEKPLLPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLKTPSNDDKIVDVVCETWNNILNNDHDLLKKSQLTELILKHFPFLTAMCYHPPKKEGKKKGHQKEQQKEKESEAQSQAEALNPSKLIEALEILVNQLHSLRNYYSHYKHKKPDAEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI |
| 688 | Cas13 | MEKPLLPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLKTPSNDDKIVDVVCETWNNILNNDHDLLKKSQLTELILKHFPFLTAMCYHPPKKEGKKKGHQKEQQKEKESEAQSQAEALNPSKLIEALEILVNQLHSLRNYYSHYKHKKPDAEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI |
| 689 | Cas13 | MDTPNFSERIPVSLQSHPYYFAHYLNMARHNAYVILEYVNRELIKPGKNLDEDNLIQSTVLKDGYFDRKPDELSHRNRLLVQHFPFLREAENEGARTCNPVSYKLKTALAALNQWRNNASHYPLNQNHEKDFDLQPFFSFAIEACKKRMREVFQPDDFYLLETNEKQFYTLHNENGFTEKGLYCFICFFLEKKYAFQFLAGIKGFKNTTDNKFRATLETFTEHCCRLPKPKLDSSDIKLDMLGELSRCPAPLFDLLDIEERKKFIREPEEVKPDESGDREEVQQVLMKRYDDRFPYFALRYFEEKNLLKGISFHIHIGRWIKSEHTKKIMGAERDRRLLKDIRTFGELKEFSPEHAPDYWLRDGITPDDVDQFSPQYRIVGNRIGIKLNYNGHNRWSVPDKEINVKPDAIISTYEFLNLFLYEHLYQKKLTGLSPAEFIQDYLDRFNNFLSEFKAGHIRPVGDFSLEKRRGQGDEPDLTARRKSLQKELDRFVLKGKDLPDKIREYLLGYKQKSEKKQAKWILGGMIKETVYWRNKAEQSPEKMRSGDMAQQLARDIIFLTPPHTVKEHKQKLNSLEYDVLQYALAYFSSNREKLYSFFKEHQLTVKGDRAHPFLYKIRLDECQGILDFFIVYMQQKEKWLGWLDRNLKSPRLNEEEFFNTYSYFIKTDTKRAIEMDYESCPNYLPRGIFNEPIAKALQKAGVKIKDEDNASYALSVYSNGKTQPFYNKERYYNKGIFRMEELPEKLQPKELLGKIQWTIKSSGKDTEEFRSLQNLKNRILNTEKEIRYVQSTDRALWIMVADLFPETFELRPDDLECIGHDLSDDLLSRPYQMKEKVYNYTITDYLPIKRYGEFRRFLKDRRLENLLTYFEEGVPLHREALVAELEAYDLQRKNLLEIIYRFEKLVFDRHRHELTFSGEGENQYVNHWDYLDFVARKYGLSAEVKELNSERFTELRNKMLHNQIPYQLWIKEAIAAREENTVCGRIMGMIGEIYERMTTEIEKQMQV |
| 690 | Cas13 | MEKPLLPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLKTPSNDDKIVDVVCETWNNILNNDHDLLKKSQLTELILKHFPFLTAMCYHPPKKEGKKKGHQKEQQKEKESEAQSQAEALNPSKLIEALEILVNQLHSLRNYYSHYKHKKPDAEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI |
| 691 | Cas13 | MENKTSLGNNIYYNPFKPQDKSYFAGYFNAAMENTDSVFRELGKRLKGKEYTSENFFDAIFKENISLVEYERYVKLLSDYFPMARLLDKKEVPIKERKENFKKNFKGIIKAVRDLRNFYTHKEHGEVEITDEIFGVLDEMLKSTVLTVKKKKVKTDKTKEILKKSIEKQLDILCQKKLEYLRDTARKIEEKRRNQRERGEKELVAPFKYSDKRDDLIAAIYNDAFDVYIDKKKDSLKESSKAKYNTKSDPQQEEGDLKIPISKNGVVFLLSLFLTKQEIHAFKSKIAGFKATVIDEATVSEATVSHGKNSICFMATHEIFSHLAYKKLKRKVRTAEINYGEAENAEQLSVYAKETLMMQMLDELSKVPDVVYQNLSEDVQKTFIEDWNEYLKENNGDVGTMEEEQVIHPVIRKRYEDKFNYFAIRFLDEFAQFPTLRFQVHLGNYLHDSRPKENLISDRRIKEKITVFGRLSELEHKKALFIKNTETNEDREHYWEIFPNPNYDFPKENISVNDKDFPIAGSILDREKQPVAGKIGIKVKLLNQQYVSEVDKAVKAHQLKQRKASKPSIQNIIEEIVPINESNPKEAIVFGGQPTAYLSMNDIHSILYEFFDKWEKKKEKLEKKGEKELRKEIGKELEKKIVGKIQAQIQQIIDKDTNAKILKPYQDGNSTAIDKEKLIKDLKQEQNILQKLKDEQTVREKEYNDFIAYQDKNREINKVRDRNHKQYLKDNLKRKYPEAPARKEVLYYREKGKVAVWLANDIKRFMPTDFKNEWKGEQHSLLQKSLAYYEQCKEELKNLLPEKVFQHLPFKLGGYFQQKYLYQFYTCYLDKRLEYISGLVQQAENFKSENKVFKKVENECFKFLKKQNYTHKELDARVQSILGYPIFLERGFMDEKPTIIKGKTFKGNEALFADWFRYYKEYQNFQTFYDTENYPLVELEKKQADRKRKTKIYQQKKNDVFTLLMAKHIFKSVFKQDSIDQFSLEDLYQSREERLGNQERARQTGERNTNYIWNKTVDLKLCDGKITVENVKLKNVGDFIKYEYDQRVQAFLKYEENIEWQAFLIKESKEEENYPYVVEREIEQYEKVRREELLKEVHLIEEYILEKVKDKEILKKGDNQNFKYYILNGLLKQLKNEDVESYKVFNLNTEPEDVNINQLKQEATDLEQKAFVLTYIRNKFAHNQLPKKEFWDYCQEKYGKIEKEKTYAEYFAEVFKKEKEALIK |
| 692 | Cas13 | MEKPLLPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLKTPSNDDKIVDVVCETWNNILNNDHDLLKKSQLTELILKHFPFLTAMCYHPPKKEGKKKGHQKEQQKEKESEAQSQAEALNPSKLIEALEILVNQLHSLRNYYSHYKHKKPDAEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI |
| 693 | Cas13 | MTEQSERPYNGTYYTLEDKHFWAAFLNLARHNAYITLTHIDRQLAYSKADITNDQDVLSFKALWKNLDNDLERKSRLRSLILKHFSFLEGAAYGKKLFESKSSGNKSSKNKELTKKEKEELQANALSLDNLKSILFDFLQKLKDFRNYYSHYRHSGSSELPLFDGNMLQRLYNVFDVSVQRVKRDHEHNDKVDPHYHFNHLVRKGKKDRYGHNDNPSFKHHFVDSEGMVTEAGLLFFVSLFLEKRDAIWMQKKIRGFKGGTGPYEQMTNEVFCRSRISLPKLKLESLRTDDWMLLDMLNELVRCPKPLYDRLREKDRACFRVPVDILPDEDDTDGGGEDPFKNTLVRHQDRFPYFALRYFDLKKVFTSLRFHIDLGTYHFAIYKKMIGEQPEDRHLTRNLYGFGRIQDFAEEHRPEEWKRLVRDLDYLETGDKPYISQTTPHYHIEKGKIGLRFVPEGQHLWPSPEVGTTRTGRSKCAQDKRLTAEAFLSVHELMPMMFYYFLLREKYSEEVSAEKVQGRIKRVIEDVYAIYDAFARDEINTLKELDTCLADKGIRRGHLPKQMITILSQERKDMKEKIRKKLQEMIADTDHRLDMLDRQTDRKIRIGRKNAGLPKSGVIADWLVRDMMRFQPVAKDASGKPLNNSKANSTEYRMLQRALALFGGEKERLTPYFRQMNLTGGNNPHPFLHETRWESHTNILSFYRSYLRARKAFLERIGRSDRVENCPFLLLKEPKTDRQTLVAGWKDEFHLPRGIFTEAVRDCLIEMGYDEVGSYREVGFMAKAVPLYFERACEDRVQPFYDSPFNVGNSLKPKKGRFLSKEDRAEEWERGMERFRDLEAWSHSAARRIKDAFAGIEYASPGNKKKIEQLLRDLSLWEAFESKLKVRADKINLAKLKKEILEAQEHPYHDFKSWQKFERELRLVKNQDIITWMMCRDLMEENKVEGLDTGTLYLKDIRPNVQEQGSLNVLNRVKPMRLPVVVYRADSRGHVHKEAPLATVYIEERNTKLLKQGNFKSFVKDRRLNGLFSFVDTGGLAMEQYPISKLRVEYELAKYQTARVCVFELTLRLEESLLSRYPHLPDESFREMLESWSDPLLAKWPELHGKVRLLIAVRNAFSHNQYPMYDEAVFSSIRKYDPSSPDAIEERMGLNIAHRLSEEVKQAKETVERIIQA |
| 694 | Cas13 | MEKPLPPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLTTPPNDDKIADVVCGTWNNILNNDHDLLKKSQLTELILKHFPFLAAMCYHPPKKEGKKKGSQKEQQKEKENEAQSQAEALNPSELIKALKTLVKQLRTLRNYYSHYKHKKPDAEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTQDFAHLNRKGKNKQDNPKFDRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQSEKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEKDKKHFQVEADGFLDEIEEEQNPFKDALIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSKQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEVKDGANRIAKYPYNSDFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGDWLVKDFMRFQPVAYDVQNQPIESSKANSTEFQLIQRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLTLSKPIRKEIKKHGRVGFISRAITLYFRERYQDDHQSFYNLPYELEAKASTPKPPLPKKREYVLRAEHYEYWQQNKPQSPTELQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELKLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYQETPIRTVYIREEQTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHASLSSLENEFRTLLEEWKKKYAASSMVTDEHIAFIASVRNAFCHNQYPFYKETLHAPILLFTVAQPTTEEKDGLGIAEALLRVLREYCEIVKSQI |
| 695 | Cas13 | MEKPLLPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLKTPSNDDKIVDVVCETWNNILNNDHDLLKKSQLTELILKHFPFLTAMCYHPPKKEGKKKGHQKEQQKEKESEAQSQAEALNPSKLIEALEILVNQLHSLRNYYSHYKHKKPDAEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI |
| 696 | Cas13 | MENKTSLGNNIYYNPFKPQDKSYFAGYFNAAMENTDSVFRELGKRLKGKEYTSENFFDAIFKENISLVEYERYVKLLSDYFPMARLLDKKEVPIKERKENFKKNFKGIIKAVRDLRNFYTHKEHGEVEITDEIFGVLDEMLKSTVLTVKKKKVKTDKTKEILKKSIEKQLDILCQKKLEYLRDTARKIEEKRRNQRERGEKELVAPFKYSDKRDDLIAAIYNDAFDVYIDKKKDSLKESSKAKYNTKSDPQQEEGDLKIPISKNGVVFLLSLFLTKQEIHAFKSKIAGFKATVIDEATVSEATVSHGKNSICFMATHEIFSHLAYKKLKRKVRTAEINYGEAENAEQLSVYAKETLMMQMLDELSKVPDVVYQNLSEDVQKTFIEDWNEYLKENNGDVGTMEEEQVIHPVIRKRYEDKFNYFAIRFLDEFAQFPTLRFQVHLGNYLHDSRPKENLISDRRIKEKITVFGRLSELEHKKALFIKNTETNEDREHYWEIFPNPNYDFPKENISVNDKDFPIAGSILDREKQPVAGKIGIKVKLLNQQYVSEVDKAVKAHQLKQRKASKPSIQNIIEEIVPINESNPKEAIVFGGQPTAYLSMNDIHSILYEFFDKWEKKKEKLEKKGEKELRKEIGKELEKKIVGKIQAQIQQIIDKDTNAKILKPYQDGNSTAIDKEKLIKDLKQEQNILQKLKDEQTVREKEYNDFIAYQDKNREINKVRDRNHKQYLKDNLKRKYPEAPARKEVLYYREKGKVAVWLANDIKRFMPTDFKNEWKGEQHSLLQKSLAYYEQCKEELKNLLPEKVFQHLPFKLGGYFQQKYLYQFYTCYLDKRLEYISGLVQQAENFKSENKVFKKVENECFKFLKKQNYTHKELDARVQSILGYPIFLERGFMDEKPTIIKGKTFKGNEALFADWFRYYKEYQNFQTFYDTENYPLVELEKKQADRKRKTKIYQQKKNDVFTLLMAKHIFKSVFKQDSIDQFSLEDLYQSREERLGNQERARQTGERNTNYIWNKTVDLKLCDGKITVENVKLKNVGDFIKYEYDQRVQAFLKYEENIEWQAFLIKESKEEENYPYVVEREIEQYEKVRREELLKEVHLIEEYILEKVKDKEILKKGDNQNFKYYILNGLLKQLKNEDVESYKVFNLNTEPEDVNINQLKQEATDLEQKAFVLTYIRNKFAHNQLPKKEFWDYCQEKYGKIEKEKTYAEYFAEVFKKEKEALIK |
| 697 | Cas13 | MFFSFHNAQRVIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI |
| 698 | Cas13 | MEKPLLPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLKTPSNDDKIVDVVCETWNNILNNDHDLLKKSQLTELILKHFPFLTAMCYHPPKKEGKKKGHQKEQQKEKESEAQSQAEALNPSKLIEALEILVNQLHSLRNYYSHYKHKKPDAEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI |
| 699 | Cas13 | MEKPLPPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLTTPPNDDKIADVVCGTWNNILNNDHDLLKKSQLTELILKHFPFLAAMCYHPPKKEGKKKGSQKEQQKEKENEAQSQAEALNPSELIKALKTLVKQLRTLRNYYSHYKHKKPDAEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTQDFAHLNRKGKNKQDNPKFDRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQSEKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEKDKKHFQVEADGFLDEIEEEQNPFKDALIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSKQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEVKDGANRIAKYPYNSDFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDVQNQPIESSKANSTEFQLIQRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLTLSKPIRKEIKKHGRVGFISRAITLYFRERYQDDHQSFYNLPYELEAKASTPKPPLPKKREYVLRAEHYEYWQQNKPQSPTELQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELKLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYQETPIRTVYIREEQTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHASLSSLENEFRTLLEEWKKKYAASSMVTDEHIAFIASVRNAFCHNQYPFYKETLHAPILLFTVAQPTTEEKDGLGIAEALLRVLREYCEIVKSQI |
| 700 | Cas13 | MEKPLLPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLKTPSNDDKIVDVVCETWNNILNNDHDLLKKSQLTELILKHFPFLTAMCYHPPKKEGKKKGHQKEQQKEKESEAQSQAEALNPSKLIEALEILVNQLHSLRNYYSHYKHKKPDAEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI |
| 701 | Cas13 | MEKPLLPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLKTPSNDDKIVDVVCETWNNILNNDHDLLKKSQLTELILKHFPFLTAMCYHPPKKEGKKKGHQKEQQKEKESEAQSQAEALNPSKLIEALEILVNQLHSLRNYYSHYKHKKPDAEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI |
| 702 | Cas13 | MEKPLLPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLKTPSNDDKIVDVVCETWNNILNNDHDLLKKSQLTELILKHFPFLTAMCYHPPKKEGKKKGHQKEQQKEKESEAQSQAEALNPSKLIEALEILVNQLHSLRNYYSHYKHKKPDAEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI |
| 703 | Cas13 | MFFSFHNAQRVIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI |
| 704 | Cas13 | MDTPNFSERIPVSLQSHPYYFAHYLNMARHNAYVILEYVNRELIKPGKNLDEDNLIQSTVLKDGYFDRKPDELSHRNRLLVQHFPFLREAENEGARTCNPVSYKLKTALAALNQWRNNASHYPLNQNHEKDFDLQPFFSFAIEACKKRMREVFQPDDFYLLETNEKQFYTLHNENGFTEKGLYCFICFFLEKKYAFQFLAGIKGFKNTTDNKFRATLETFTEHCCRLPKPKLDSSDIKLDMLGELSRCPAPLFDLLDIEERKKFIREPEEVKPDESGDREEVQQVLMKRYDDRFPYFALRYFEEKNLLKGISFHIHIGRWIKSEHTKKIMGAERDRRLLKDIRTFGELKEFSPEHAPDYWLRDGITPDDVDQFSPQYRIVGNRIGIKLNYNGHNRWSVPDKEINVKPDAIISTYEFLNLFLYEHLYQKKLTGLSPAEFIQDYLDRFNNFLSEFKAGHIRPVGDFSLEKRRGQGDEPDLTARRKSLQKELDRFVLKGKDLPDKIREYLLGYKQKSEKKQAKWILGGMIKETVYWRNKAEQSPEKMRSGDMAQQLARDIIFLTPPHTVKEHKQKLNSLEYDVLQYALAYFSSNREKLYSFFKEHQLTVKGDRAHPFLYKIRLDECQGILDFFIVYMQQKEKWLGWLDRNLKSPRLNEEEFFNTYSYFIKTDTKRAIEMDYESCPNYLPRGIFNEPIAKALQKAGVKIKDEDNASYALSVYSNGKTQPFYNKERYYNKGIFRMEELPEKLQPKELLGKIQWTIKSSGKDTEEFRSLQNLKNRILNTEKEIRYVQSTDRALWIMVADLFPETFELRPDDLECIGHDLSDDLLSRPYQMKEKVYNYTITDYLPIKRYGEFRRFLKDRRLENLLTYFEEGVPLHREALVAELEAYDLQRKNLLEIIYRFEKLVFDRHRHELTFSGEGENQYVNHWDYLDFVARKYGLSAEVKELNSERFTELRNKMLHNQIPYQLWIKEAIAAREENTVCGRIMGMIGEIYERMTTEIEKQMQV |
| 705 | Cas13 | MTEQNEKPYNGTYYTLEDKHFWAAFLNLARHNAYITLTHIDRQLAYSKADITNDEDILFFKGQWKNLDNDLERKARLRSLILKHFFFLEGAAYGKKLFESQSSGNKSSKKKELTKKEKEELQANALSLDNLKSILFDFLQKLKDFRNYYSHYRHPESSELPLFDGNMLQRLYNVFDVSVQRVKRDHEHNDKVDPHRHFNHLVRKGKKDKYGNNDNPFFKHHFVDREGKVTEAGLLFFVSLFLEKRDAIWMQKKIRGFKGGTETYQQMTNEVFCRSRISLPKLKLESLRTDDWMLLDMLNELVRCPKSLYDRLREEDRARFRVPVDILSDEDDTDGAEEDPFKNTLVRHQDRFPYFALRYFDLKKVFTSLRFHIDLGTYHFAIYKKNIGEQPEDRHLTRNLYGFGRIQDFAEEHRPEEWKRLVRDLDYFETGDKPYITQTTPHYHIEKGKIGLRFVPEGQHLWPSPEVGATRTGRSKYAQDKRFTAEAFLSVHELMPMMFYYFLLREKYSEEASAERVQGRIKRVIEDVYAVYDAFARDEINTRDELDACLADKGIRRGHLPRQMIAILSQKHKDMEEKVRKKLQEMIADTDHRLDMLDRQTDRKIRIGRKNAGLPKSGVIADWLVRDMMRFQPVAKDTSGKPLNNSKANSTEYRMLQRALALFGGEKERLTPYFRQMNLTGGNNPHPFLHETRWESHTNILSFYRSYLKARKAFLQSIGRSDRVENHRFLLLKEPKTDRQTLVAGWKGEFHLPRGIFTEAVRDCLIEMGLDEVRSYKEVGFMAKAVPLYFERASKDRVQPFYDYPFNVGNSLKPKKGRFLSKEKRAEEWESGKERFRDLEAWSHSAARRIEDAFAGIENASRENKKKIEQLLQDLSLWETFESKLKVKADKINIAKLKKEILEAKEHPYLDFKSWQKFERELRLVKNQDIITWMMCRDLMEENKVEGLDTGTLYLKDIRTDVQEQGSLNVLNHVKPMRLPVVVYRADSRGHVHKEQAPLATVYIEERDTKLLKQGNFKSFVKDRRLNGLFSFVDTGGLAMEQYPISKLRVEYELAKYQTARVCAFEQTLELEESLLTRYPHLPDKNFRKMLESWSDPLLAKWPELHEKVRLLIAVRNAFSHNQYPMYDEAVFSPIRKYDPSSPDAIEERMRLNIAHRLSEEVKQAKETVERIIQA |
| 706 | Cas13 | MDTPNFSERIPVSLQSHPYYFAHYLNMARHNAYVILEYVNRELIKPGKNLDEDNLIQSTVLKDGYFDRKPDELSHRNRLLVQHFPFLREAENEGARTCNPVSYKLKTALAALNQWRNNASHYPLNQNHEKDFDLQPFFSFAIEACKKRMREVFQPDDFYLLETNEKQFYTLHNENGFTEKGLYCFICFFLEKKYAFQFLAGIKGFKNTTDNKFRATLETFTEHCCRLPKPKLDSSDIKLDMLGELSRCPAPLFDLLDIEERKKFIREPEEVKPDESGDREEVQQVLMKRYDDRFPYFALRYFEEKNLLKGISFHIHIGRWIKSEHTKKIMGAERDRRLLKDIRTFGELKEFSPEHAPDYWLRDGITPDDVDQFSPQYRIVGNRIGIKLNYNGHNRWSVPDKEINVKPDAIISTYEFLNLFLYEHLYQKKLTGLSPAEFIQDYLDRFNNFLSEFKAGHIRPVGDFSLEKRRGQGDEPDLTARRKSLQKELDRFVLKGKDLPDKIREYLLGYKQKSEKKQAKWILGGMIKETVYWRNKAEQSPEKMRSGDMAQQLARDIIFLTPPHTVKEHKQKLNSLEYDVLQYALAYFSSNREKLYSFFKEHQLTVKGDRAHPFLYKIRLDECQGILDFFIVYMQQKEKWLGWLDRNLKSPRLNEEEFFNTYSYFIKTDTKRAIEMDYESCPNYLPRGIFNEPIAKALQKAGVKIKDEDNASYALSVYSNGKTQPFYNKERYYNKGIFRMEELPEKLQPKELLGKIQWTIKSSGKDTEEFRSLQNLKNRILNTEKEIRYVQSTDRALWIMVADLFPETFELRPDDLECIGHDLSDDLLSRPYQMKEKVYNYTITDYLPIKRYGEFRRFLKDRRLENLLTYFEEGVPLHREALVAELEAYDLQRKNLLEIIYRFEKLVFDRHRHELTFSGEGENQYVNHWDYLDFVARKYGLSAEVKELNSERFTELRNKMLHNQIPYQLWIKEAIAAREENTVCGRIMGMIGEIYERMTTEIEKQMQV |
| 707 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEMEHLRRYTRKKGRVVLKTEDDHFYYTLANNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSRIPIELYQTLEPKYREMYNATLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQEKEEAIFLPRGLFNEAIINCLKKSKLKQLIESPTREKSPALNVSYLILNYFRTYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRINSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDFSNDSSA |
| 708 | Cas13 | MEKPLPPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLTTPPNDDKIADVVCGTWNNILNNDHDLLKKSQLTELILKHFPFLAAMCYHPPKKEGKKKGSQKEQQKEKENEAQSQAEALNPSELIKALKTLVKQLRTLRNYYSHYKHKKPDAEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTQDFAHLNRKGKNKQDNPKFDRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQSEKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEKDKKHFQVEADGFLDEIEEEQNPFKDALIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSKQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEVKDGANRIAKYPYNSDFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDVQNQPIESSKANSTEFQLIQRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLTLSKPIRKEIKKHGRVGFISRAITLYFRERYQDDHQSFYNLPYELEAKASTPKPPLPKKREYVLRAEHYEYWQQNKPQSPTELQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELKLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYQETPIRTVYIREEQTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHASLSSLENEFRTLLEEWKKKYAASSMVTDEHIAFIASVRNAFCHNQYPFYKETLHAPILLFTVAQPTTEEKDGLGIAEALLRVLREYCEIVKSQI |
| 709 | Cas13 | MEKPLLPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLKTPSNDDKIVDVVCETWNNILNNDHDLLKKSQLTELILKHFPFLTAMCYHPPKKEGKKKGHQKEQQKEKESEAQSQAEALNPSKLIEALEILVNQLHSLRNYYSHYKHKKPDAEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI |
| 710 | Cas13 | MEKPLLPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLKTPSNDDKIVDVVCETWNNILNNDHDLLKKSQLTELILKHFPFLTAMCYHPPKKEGKKKGHQKEQQKEKESEAQSQAEALNPSKLIEALEILVNQLHSLRNYYSHYKHKKPDAEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI |
| 711 | Cas13 | MFFSFHNAQRVIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI |
| 712 | Cas13 | MEKPLLPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLKTPSNDDKIVDVVCETWNNILNNDHDLLKKSQLTELILKHFPFLTAMCYHPPKKEGKKKGHQKEQQKEKESEAQSQAEALNPSKLIEALEILVNQLHSLRNYYSHYKHKKPDAEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI |
| 713 | Cas13 | MEKPLLPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLKTPSNDDKIVDVVCETWNNILNNDHDLLKKSQLTELILKHFPFLTAMCYHPPKKEGKKKGHQKEQQKEKESEAQSQAEALNPSKLIEALEILVNQLHSLRNYYSHYKHKKPDAEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI |
| 714 | Cas13 | MEKPLLPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLKTPSNDDKIVDVVCETWNNILNNDHDLLKKSQLTELILKHFPFLTAMCYHPPKKEGKKKGHQKEQQKEKESEAQSQAEALNPSKLIEALEILVNQLHSLRNYYSHYKHKKPDAEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI |
| 715 | Cas13 | MDTPNFSERIPVSLQSHPYYFAHYLNMARHNAYVILEYVNRELIKPGKNLDEDNLIQSTVLKDGYFDRKPDELSHRNRLLVQHFPFLREAENEGARTCNPVSYKLKTALAALNQWRNNASHYPLNQNHEKDFDLQPFFSFAIEACKKRMREVFQPDDFYLLETNEKQFYTLHNENGFTEKGLYCFICFFLEKKYAFQFLAGIKGFKNTTDNKFRATLETFTEHCCRLPKPKLDSSDIKLDMLGELSRCPAPLFDLLDIEERKKFIREPEEVKPDESGDREEVQQVLMKRYDDRFPYFALRYFEEKNLLKGISFHIHIGRWIKSEHTKKIMGAERDRRLLKDIRTFGELKEFSPEHAPDYWLRDGITPDDVDQFSPQYRIVGNRIGIKLNYNGHNRWSVPDKEINVKPDAIISTYEFLNLFLYEHLYQKKLTGLSPAEFIQDYLDRFNNFLSEFKAGHIRPVGDFSLEKRRGQGDEPDLTARRKSLQKELDRFVLKGKDLPDKIREYLLGYKQKSEKKQAKWILGGMIKETVYWRNKAEQSPEKMRSGDMAQQLARDIIFLTPPHTVKEHKQKLNSLEYDVLQYALAYFSSNREKLYSFFKEHQLTVKGDRAHPFLYKIRLDECQGILDFFIVYMQQKEKWLGWLDRNLKSPRLNEEEFFNTYSYFIKTDTKRAIEMDYESCPNYLPRGIFNEPIAKALQKAGVKIKDEDNASYALSVYSNGKTQPFYNKERYYNKGIFRMEELPEKLQPKELLGKIQWTIKSSGKDTEEFRSLQNLKNRILNTEKEIRYVQSTDRALWIMVADLFPETFELRPDDLECIGHDLSDDLLSRPYQMKEKVYNYTITDYLPIKRYGEFRRFLKDRRLENLLTYFEEGVPLHREALVAELEAYDLQRKNLLEIIYRFEKLVFDRHRHELTFSGEGENQYVNHWDYLDFVARKYGLSAEVKELNSERFTELRNKMLHNQIPYQLWIKEAIAAREENTVCGRIMGMIGEIYERMTTEIEKQMQV |
| 716 | Cas13 | MFFSFHNAQRVIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI |
| 717 | Cas13 | MEKPLLPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLKTPSNDDKIVDVVCETWNNILNNDHDLLKKSQLTELILKHFPFLTAMCYHPPKKEGKKKGHQKEQQKEKESEAQSQAEALNPSKLIEALEILVNQLHSLRNYYSHYKHKKPDAEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI |
| 718 | Cas13 | MTNTPKRRTLHRHPSYFGAFLNIARHNAFMIMEHLSTKYDMEDKNTLDEAQLPNAKLFGCLKKRYGKPDVTEGVSRDLRRYFPFLNYPLFLHLEKQQNAEQAATYDINPEDIEFTLKGFFRLLNQMRNNYSHYISNTDYGKFDKLPVQDIYEAAIFRLLDRGKHTKRFDVFESKHTRHLESNNSEYRPRSLANSPDHENTVAFVTCLFLERKYAFPFLSRLDCFRSTNDAAEGDPLIRKASHECYTMFCCRLPQPKLESSDILLDMVNELGRCPSALYNLLSEEDQARFHIKREEITGFEEDPDEELEQEIVLKRHSDRFPYFALRYFDDTEAFQTLRFDVYLGRWRTKPVYKKRIYGQERDRVLTQSIRTFTRLSRLLPIYENVKHDAVRQNEEDGKLVNPDVTSQFHKSWIQIESDDRAFLSDRIEHFSPHYNFGDQVIGLKFINPDRYAAIQNVFPKLPGEEKKDKDAKLVNETADAIISTHEIRSLFLYHYLSKKPISAGDERRFIQVDTETFIKQYIDTIKLFFEDIKSGELQPIADPPNYQKNEPLPYVRGDKEKTQEERAQYRERQKEIKERRKELNTLLQNRYGLSIQYIPSRLREYLLGYKKVPYEKLALQKLRAQRKEVKKRIKDIEKMRTPRVGEQATWLAEDIVFLTPPKMHTPERKTTKHPQKLNNDQFRIMQSSLAYFSVNKKAIKKFFQKETGIGLSNRETSHPFLYRIDVGRCRGILDFYTGYLKYKMDWLDDAIKKVDNRKHGKKEAKKYEKYLPSSIQHKTPLELDYTRLPVYLPRGLFKKAIVKALAAHADFQVEPEEDNVIFCLDQLLDGDTQDFYNWQRYYRSALTEKETDNQLVLAHPYAEQILGTIKTLEGKQKNNKLGNKAKQKIKDELIDLKRAKRRLLDREQYLRAVQAEDRALWLMIQERQKQKAEHEEIAFDQLDLKNITKILTESIDARLRIPDTKVDITDKLPLRRYGDLRRVAKDRRLVNLASYYHVAGLSEIPYDLVKKELEEYDRRRVAFFEHVYQFEKEVYDRYAAELRNENPKGESTYFSHWEYVAVAVKHSADTHFNELFKEKVMQLRNKFHHNEFPYFDWLLPEVEKASAALYADRVFDVAEGYYQKMRKLMRQ |
| 719 | Cas13 | MTNTPKRRTLHRHPSYFGAFLNIARHNAFMIMEHLSTKYDMEDKNTLDEAQLPNAKLFGCLKKRYGKPDVTEGVSRDLRRYFPFLNYPLFLHLEKQQNAEQAATYDINPEDIEFTLKGFFRLLNQMRNNYSHYISNTDYGKFDKLPVQDIYEAAIFRLLDRGKHTKRFDVFESKHTRHLESNNSEYRPRSLANSPDHENTVAFVTCLFLERKYAFPFLSRLDCFRSTNDAAEGDPLIRKASHECYTMFCCRLPQPKLESSDILLDMVNELGRCPSALYNLLSEEDQARFHIKREEITGFEEDPDEELEQEIVLKRHSDRFPYFALRYFDDTEAFQTLRFDVYLGRWRTKPVYKKRIYGQERDRVLTQSIRTFTRLSRLLPIYENVKHDAVRQNEEDGKLVNPDVTSQFHKSWIQIESDDRAFLSDRIEHFSPHYNFGDQVIGLKFINPDRYAAIQNVFPKLPGEEKKDKDAKLVNETADAIISTHEIRSLFLYHYLSKKPISAGDERRFIQVDTETFIKQYIDTIKLFFEDIKSGELQPIADPPNYQKNEPLPYVRGDKEKTQEERAQYRERQKEIKERRKELNTLLQNRYGLSIQYIPSRLREYLLGYKKVPYEKLALQKLRAQRKEVKKRIKDIEKMRTPRVGEQATWLAEDIVFLTPPKMHTPERKTTKHPQKLNNDQFRIMQSSLAYFSVNKKAIKKFFQKETGIGLSNRETSHPFLYRIDVGRCRGILDFYTGYLKYKMDWLDDAIKKVDNRKHGKKEAKKYEKYLPSSIQHKTPLELDYTRLPVYLPRGLFKKAIVKALAAHADFQVEPEEDNVIFCLDQLLDGDTQDFYNWQRYYRSALTEKETDNQLVLAHPYAEQILGTIKTLEGKQKNNKLGNKAKQKIKDELIDLKRAKRRLLDREQYLRAVQAEDRALWLMIQERQKQKAEHEEIAFDQLDLKNITKILTESIDARLRIPDTKVDITDKLPLRRYGDLRRVAKDRRLVNLASYYHVAGLSEIPYDLVKKELEEYDRRRVAFFEHVYQFEKEVYDRYAAELRNENPKGESTYFSHWEYVAVAVKHSADTHFNELFKEKVMQLRNKFHHNEFPYFDWLLPEVEKASAALYADRVFDVAEGYYQKMRKLMRQ |
| 720 | Cas13 | MEKPLLPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLKTPSNDDKIVDVVCETWNNILNNDHDLLKKSQLTELILKHFPFLTAMCYHPPKKEGKKKGHQKEQQKEKESEAQSQAEALNPSKLIEALEILVNQLHSLRNYYSHYKHKKPDAEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI |
| 721 | Cas13 | MEKPLLPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLKTPSNDDKIVDVVCETWNNILNNDHDLLKKSQLTELILKHFPFLTAMCYHPPKKEGKKKGHQKEQQKEKESEAQSQAEALNPSKLIEALEILVNQLHSLRNYYSHYKHKKPDAEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI |
| 722 | Cas13 | MEKPLLPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLKTPSNDDKIVDVVCETWNNILNNDHDLLKKSQLTELILKHFPFLTAMCYHPPKKEGKKKGHQKEQQKEKESEAQSQAEALNPSKLIEALEILVNQLHSLRNYYSHYKHKKPDAEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI |
| 723 | Cas13 | MESIKNSQKSTGKTLQKDPPYFGLYLNMALLNVRKVENHIRKWLGDVALLPEKSGFHSLLTTDNLSSAKWTRFYYKSRKFLPFLEMFDSDKKSYENRRETTECLDTIDRQKISSLLKEVYGKLQDIRNAFSHYHIDDQSVKHTALIISSEMHRFIENAYSFALQKTRARFTGVFVETDFLQAEEKGDNKKFFAIGGNEGIKLKDNALIFLICLFLDREEAFKFLSRATGFKSTKEKGFLAVRETFCALCCRQPHERLLSVNPREALLMDMLNELNRCPDILFEMLDEKDQKSFLPLLGEEEQAHILENSLNDELCEAIDDPFEMIASLSKRVRYKNRFPYLMLRYIEEKNLLPFIRFRIDLGCLELASYPKKMGEENNYERSVTDHAMAFGRLTDFHNEDAVLQQITKGITDEVRFSLYAPRYAIYNNKIGFVRTGGSDKISFPTLKKKGGEGHCVAYTLQNTKSFGFISIYDLRKILLLSFLDKDKAKNIVSGLLEQCEKHWKDLSENLFDAIRTELQKEFPVPLIRYTLPRSKGGKLVSSKLADKQEKYESEFERRKEKLTEILSEKDFDLSQIPRRMIDEWLNVLPTSREKKLKGYVETLKLDCRERLRVFEKREKGEHPVPPRIGEMATDLAKDIIRMVIDQGVKQRITSAYYSEIQRCLAQYAGDDNRRHLDSIIRELRLKDTKNGHPFLGKVLRPGLGHTEKLYQRYFEEKKEWLEATFYPAASPKRVPRFVNPPTGKQKELPLIIRNLMKERPEWRDWKQRKNSHPIDLPSQLFENEICRLLKDKIGKEPSGKLKWNEMFKLYWDKEFPNGMQRFYRCKRRVEVFDKVVEYEYSEEGGNYKKYYEALIDEVVRQKISSSKEKSKLQVEDLTLSVRRVFKRAINEKEYQLRLLCEDDRLLFMAVRDLYDWKEAQLDLDKIDNMLGEPVSVSQVIQLEGGQPDAVIKAECKLKDVSKLMRYCYDGRVKGLMPYFANHEATQEQVEMELRHYEDHRRRVFNWVFALEKSVLKNEKLRRFYEESQGGCEHRRCIDALRKASLVSEEEYEFLVHIRNKSAHNQFPDLEIGKLPPNVTSGFCECIWSKYKAIICRIIPFIDPERRFFGKLLEQK |
| 724 | Cas13 | MEKPLPPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLTTPPNDDKIADVVCGTWNNILNNDHDLLKKSQLTELILKHFPFLAAMCYHPPKKEGKKKGSQKEQQKEKEKEKENEAQSQAEALNPSELIKVLKTIVKQLRTLRNYYSHHSHKKPDTEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSHILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDIMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYQETPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLDLEEKLLKKHTSLSSLENKFRILLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYEEALHAPIPLFTVAQQTTEEKDGLGIAEALLRVLREYCEIVKSQI |
| 725 | Cas13 | MFFSFHNAQRVIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI |
| 726 | Cas13 | MEKPLPPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLTTPPNDDKIADVVCGTWNNILNNDHDLLKKSQLTELILKHFPFLAAMCYHPPKKEGKKKGSQKEQQKEKEKEKENEAQSQAEALNPSELIKVLKTIVKQLRTLRNYYSHHSHKKPDTEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSHILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDIMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYQETPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLDLEEKLLKKHTSLSSLENKFRILLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYEEALHAPIPLFTVAQQTTEEKDGLGIAEALLRVLREYCEIVKSQI |
| 727 | Cas13 | MGAIENKHIFAAYANLAIDGLIKTLNFIAKKLDTQKQLSSWDIKHVITLIDSIFDQNPQNNLEQVVEGYLPWIKPIIEMKTPKKGERQSDKLCIEYKTIITAFASLLNDVRNYYTHYYHDPICIYPGGYDIPSSLNCIYDSAINIIKERFQAEEKEIEHLRRYTRKKGRVVLKTEDDHFYYTLVNNNDLSEKGYAFFISMFLERKYSYLFLKKLSGFKRGDSLQYRLTLEVFTALSTKPPVERLRTTKDTKQDRALDILNELSRIPIELYQTLEPKYREMYNETLQPTDAEDPYGLPDRSRIRFRSRFEAFALHFLDKQADFKEIGFYTYLGNYFHNGYQKTRVDRETKDRYINFQLAGFCKNIQDISAKKLSEALNVKSIDISTDSIPDINSFEPYLVQSTPHYIVNGNNIGIKVLPEGKDTYPTIDEKGAKMPIADFWLSKYELPAMLFYTYLRNNNIHKSHCPLSVKDIIERSIHKSTKQKHPEERSELMLRRVMKAIFWTDSKLNEVERIKSQKSAFGKRQHEILKAGRIAETLVRDMLWLQPSKNNGRDKVTEPNFQAIQVSLAYFGIRRNDLTEIFTRAGLINSSNPHPFLAQIGTNYTSLIEFYIAYLKERKVYFSRIQKKILQGKLNIQCHPLRDLQREPNKPQDKEEAIFLPRGLFNEAIINCLKKSKLKQLIESPTREKSPALNVSYLIQNYFRTYFEDQSQEFYAQPRNYRLFDKLSPNKGKSKSYLSLEQRIKKMEELRPSKIPVAEANKLLEKEDRLYRKNYNEICDNESIIRLYQIQDILLFMMTKEYLPSDLYNRINKYKLENVKGILNERVSYLIDLNPLKIQGEDIKIKDYGKLFYIHHDTRISSLNKVLSKVKRNNSISSSVKIQPYENYKRECLDFEEAQIQIIPIIHSFEIAMVSMFPDLKKATPGNYYDFNELITEYEKRTKQKIDSSFLIKTRNMFLHDKYEAECIKEISDDFVYAKKIIAEFKMKIENIKLEDLSNDSSA |
| 728 | Cas13 | MEKPLLPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLKTPSNDDKIVDVVCETWNNILNNDHDLLKKSQLTELILKHFPFLTAMCYHPPKKEGKKKGHQKEQQKEKESEAQSQAEALNPSKLIEALEILVNQLHSLRNYYSHYKHKKPDAEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKDWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI |
| 729 | Cas13 | MEKPLPPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLTTPPNDDKIADVVCGTWNNILNNDHDLLKKSQLTELILKHFPFLAAMCYHPPKKEGKKKGSQKEQQKEKENEAQSQAEALNPSELIKALKTLVKQLRTLRNYYSHYKHKKPDAEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTQDFAHLNRKGKNKQDNPKFDRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQSEKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEKDKKHFQVEADGFLDEIEEEQNPFKDALIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSKQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEVKDGANRIAKYPYNSDFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDVQNQPIESSKANSTEFQLIQRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLTLSKPIRKEIKKHGRVGFISRAITLYFRERYQDDHQSFYNLPYELEAKASTPKPPLPKKREYVLRAEHYEYWQQNKPQSPTELQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELKLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYQETPIRTVYIREEQTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHASLSSLENEFRTLLEEWKKKYAASSMVTDEHIAFIASVRNAFCHNQYPFYKETLHAPILLFTVAQPTTEEKDGLGIAEALLRVLREYCEIVKSQI |
| 730 | Cas13 | MEKPLLPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLKTPSNDDKIVDVVCETWNNILNNDHDLLKKSQLTELILKHFPFLTAMCYHPPKKEGKKKGHQKEQQKEKESEAQSQAEALNPSKLIEALEILVNQLHSLRNYYSHYKHKKPDAEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI |
| 731 | Cas13 | MEKPLLPNVYTLKHKFFWGAFLNIARHNAFITICHINEQLGLKTPSNDDKIVDVVCETWNNILNNDHDLLKKSQLTELILKHFPFLTAMCYHPPKKEGKKKGHQKEQQKEKESEAQSQAEALNPSKLIEALEILVNQLHSLRNYYSHYKHKKPDAEKDIFKHLYKAFDASLRMVKEDYKAHFTVNLTRDFAHLNRKGKNKQDNPDFNRYRFEKDGFFTESGLLFFTNLFLDKRDAYWMLKKVSGFKASHKQREKMTTEVFCRSRILLPKLRLESRYDHNQMLLDMLSELSRCPKLLYEKLSEENKKHFQVEADGFLDEIEEEQNPFKDTLIRHQDRFPYFALRYLDLNESFKSIRFQVDLGTYHYCIYDKKIGDEQEKRHLTRTLLSFGRLQDFTEINRPQEWKALTKDLDYKETSNQPFISKTTPHYHITDNKIGFRLGTSKELYPSLEIKDGANRIAKYPYNSGFVAHAFISVHELLPLMFYQHLTGKSEDLLKETVRHIQRIYKDFEEERINTIEDLEKANQGRLPLGAFPKQMLGLLQNKQPDLSEKAKIKIEKLIAETKLLSHRLNTKLKSSPKLGKRREKLIKTGVLADWLVKDFMRFQPVAYDAQNQPIKSSKANSTEFWFIRRALALYGGEKNRLEGYFKQTNLIGNTNPHPFLNKFNWKACRNLVDFYQQYLEQREKFLEAIKNQPWEPYQYCLLLKIPKENRKNLVKGWEQGGISLPRGLFTEAIRETLSEDLMLSKPIRKEIKKHGRVGFISRAITLYFKEKYQDKHQSFYNLSYKLEAKAPLLKREEHYEYWQQNKPQSPTESQRLELHTSDRWKDYLLYKRWQHLEKKLRLYRNQDVMLWLMTLELTKNHFKELNLNYHQLKLENLAVNVQEADAKLNPLNQTLPMVLPVKVYPATAFGEVQYHKTPIRTVYIREEHTKALKMGNFKALVKDRRLNGLFSFIKEENDTQKHPISQLRLRRELEIYQSLRVDAFKETLSLEEKLLNKHTSLSSLENEFRALLEEWKKEYAASSMVTDEHIAFIASVRNAFCHNQYPFYKEALHAPIPLFTVAQPTTEEKDGLGIAEALLKVLREYCEIVKSQI |
| 732 | Cas12a | MKSLAQFQNLYALQKTLRFELKPEGHTRETFNRWLEEIEKEQASENENIVYQDLLRAKKYEKIKIILDEYHKDFIEQALAYANLTELEKYEELYRKSNRTSEEEEEFENTKESLRKQIANIFIKNPNKTVQERWKFLFSKKLIQNELIVWVKGNYELLSEKLKNEFPDESSIISTIEDFKYFTTYFRNYHENRKNLYSNEDKFSTIAHRLIHENLPKFIDNIAIYQKAKAVLNINEVEKELGLPEDTLDKIFSLDFFSKALTQKGIDQYNYFLGGKTENEVKKIKGLNEFINLYNQQQQDKNQRLPFLKVLYKLPLFERTSTSFRFEPIENDRDLIERIGKFYYNDLKQYRDDSQGDTTDILSGINTLLRHVHDYRDGLYVNGGITLTQISQKIFGSWSYINNALAYFYDTYIDASGVDHQGERKPKKQKQIQEKTKWLKQKQFPVILVEKALSEYKSIETNEDLKTRISDTTLCDFFKRCGNDDNGQDLFDRIEARLREKNEEGYSLEDLLKKEFTTERKLMQDKTKTLLIKNFLDVIQGDKDDITAGLLHFVKCLIPRTEISEKNELFYSGMEKYLNILSEVTPLYNKARNYLTQKPYSIEKVKLNFENSTLLDGWDENEESDNSCVLLRKRGYYYLGIMNKKHNMIFDRKIYPKATEGEAYYEKMIYKLLPGAYKMLPKVFFSEKNIDYFKPSEEILRIRNTASYSKNGQPQEGYQKASFSIEDCRKYIDFFKKCIANHWDWQKFNFNFSPTEYYQSIDEFYREIERQGYKIDFVKIPESYINQLIKENKLYLFKIYNKDFSEKKKSKGKDNLHTLYWKMLFDEKNLKDVVLKLNGEAEVFFRQKSILYNEEIWNKGHHYSELKDRFSYPIISNKRYAEDKFFLHVPITLNFKADGINNVNNMVNEFIKDNRDIHIIGIDRGERHLLYVSVINQKGDIVEQCSLNEIVTEYNGKIFKKNYHEELDNLEKERDRARKDWQTIANIKELKEGYLSHVIHKISKLILKYNAIVVMEDLNSGFKRGRQKVEKQVYQNFEKQLIEKLNYLVLKESNVDEPGGVLRAYQLANKFETFKKLGKQSGIIFYVPAAYTSAIDPVTGYIQYLYPLKQADSVEKARKFYSQFKRISYNPHKQWFEFSFDYNDFNIIYHGKSSWTICTTNTERYMWNRLLNNGHGGEELVYVTNELELLFGEYNIIYGDGKDIKQQITDVQDIDVDRTAKQFYKRINELLNLTLKLRHNNGKKGADEEDYILSPVEPYFDSRFESRKPSMQQTLPINADANGAFNIARKGLLLLERLNQLGVEEFEKTKKSNNKKTQWLPHELWVEYAQNHTRK |
| 733 | Cas12a | MQDKTGWSSFTNKYSLSKTLRFELKPVGNTQKMLEDDGVFQKDRERQENYKKVKPFMDKLHREFIKEALNNLKLEGLTEYFEIFKKFRKDKNNKELKNAEKKLRQIIGRCYTETAQIWVEKYKEFGFKKKNIGFLFEEGVFELMKLKYGNDEASQIEKNGEVLSIFDGWKGFLGYFKKFFETRNNFYKDDGTSTAVSTRIINENLKIYLDNLIKYNKIKDKVDFKEADILQENKLNLSDFFNVESYAKYSLQKGIDYYNEILGGKTLKNGTKLKGLNEVINEYKQKNKSGELSKFKMLKKQILGEGEDRTLFEEIENEDELKDVLKDFFYNADPKITLFKTLLEDFFSNTEKYKDELDKIYFNTVAINGILHRWVDDSGVFQKYLFEVLKSNKLVKSNHYDKKEDSYKFPDFISFEHIKVALENCERDGLKDKFWKEKYYTKECLTENGLANLWQEFLEIYKCEFKKLYDYKTDDNDCYLQYRDNYKKYILDANFNPKEKSAKDIIKDYLDSVLSIYQLAKYFALEKKKVWTTDYETGDFYYEYIKFYEDTYEQIIKPYNLVRNYLTRKPINTAKKWKLNFDNAYLASGWDKDKEVSNLTVILRRDEQYYLAIMKKGKNKIFEKKFSCGEFEKMEYKQIAEASSDIHNLVLMNDGSCRRCIKMHDKRKYWPLDISIIKEKKSYAKENFVRRDFERFVNYMKKCSLLYWKEYDLKFSDTSTYKNINDFTNEIASQGYKLSFSAIPESYINEKNNNGELYLFQIYNKDFGIKTEGNKNLHTMYWESIFSEENRFRNFIVKLNGKAEIFYRPKSEQVEKEQRNFTREIIKNRRYTENKIYFHCPITLNRISRENVKKFNNGINNYIATNPNINILGVDRGEKHLVYYAIVDQDGKLIDAEDATGSFNTIGSTDYHRLLEEKAKDREKERKDWDLIRGIKDLKKGYISLVVRKIADLAIKYNAIIIFEDLNTRFKQIRGGMEKSVYQQLEKALINKLSFLVNKGEKDPEQAGHLLKAYQLAAPFQTFDKMGRQTGIIFYTQASYTSKIDPITGWRPNLYLKYRNIDDSKESIKKFKSILFNKEKNRFEFTYDLKDFVDFEEDKIPEKTEWTLCSSVERHKWNRHMNNNKGGYEVYKDLTENFYKLFDENNISMNKDIVDQVESISNGNFFRQFIYLFNLVCQIRNTDEKAEDVDKRDFILSPVEPFFDSRRAKDFKAYGDNLPKNGDENGAYNIARKGVLIIKKIKEYYNQNGSCDKLGWGDLSISHKEWDDFATNN |
| 734 | Cas12a | MDSYEQFTKLYPIQKTIRFELKPQGRTKEHFDNSNFLEKDRERDDNYKILKEVIDDYHREFIDECLSNIQLNWDDLKKFSEEYRRSKEKKNNRDSESEQKRMSTTSETRAINKKNLEAEQKRMRGEIVSAFKKDDRFKHLFSEKLFSILLKNQIYEKGTLEEIEAFDCFNKFSGYFKSFHENRKNMYSDEDKETAISYRIINENFPKLLDNFEKYQYVCREYPEQIREAESTLAEAGCYIKMDEIFSIDNFNNVMMQGGKESGISRYNLAIGGIVQGTGEKPKGLNEFLNLAYQNEPNGRKKIRMEPLYKQILSKEESFSYRLEAFTDDSQLLSAIRSFFDIVEKDKNGNIFDRAVNLMSSFSNYDTSKIYIRKAYLNQVSKEIFGYRGKSDSKPAKTADESLNKSGGWEKLGQMLRDYKADSIGDRNLEKTCKKVDKWLDSDEFTLSDILGAISLAGSNETFEAYVSEICVARRNIDKEKEKEKNINVEKISGDTESIQIIKALLDSVQEFFHLLSPFQLHPNTPHDWTFYAEFNDIYDKLSAITPLYNQARNHLTKKNLDTSKIKLNFNNPTLANGWDVNKEYENTAVILIRDGKYYLGIMNPKNKRKIKFDEGSGAGPFYQKMVYKLLPGPYRMLPKVFFAKKNIDYYNPSQEIREGYKAGKHKKGKEFDKGFCHKLIDFFKESIQKNENWKVFDFKFSPTESYDDISEFYQEVEKQGYRMYFVNIPSDTIDRYVEGGDMFLFQIYNKDFAKGAKGNKDMHTLYWNAVFSEENLQKGVMKLSGEAELFYRKKSDIKDPPHREGEILVNRTYIDRTHVSGVMGEQNTVKESRIPVPDEIHKNLFDYYNHGRELTKEEKEYCDKVGSFKAYYGIVKDRRYLENKMYFHVPLTLNFKAIGEKRINKMAIEKFLTDENACIIGIDRGERNLLYYSIIDRNGKIIDQKSLNVIDGFDYHEKLSQRQTEREVARQSWNSIGKIKDLKEGYLAKAVHEISKMAIKYNAIVVLEDLHFGFKKGRLKVEKQIYQKFEEMLINKLNYLVFKDVSDSSDAGGVLNAYQLTAPLESFSKLGKQSGILFYVPAAFTSVIDPTTGFVDLFNSSSITSTQKKKEFLQRFESIVYSARDGGIFAFTFDYRNFSKIATDHRNMWTVYTHGERIRYVRDEKCYKTTDPTKRIKEALSGIEYDDGSDIRDKITQSGDNNLINTVYHSFMDTIKMRNKDGRIDYIISPVKNRNGEFFRSDYKHRDFPVDADANGAYHIALKGELLMRMIGKTYDSNSDKMPKLEHKDWFEFMQTRGDQ |
| 735 | Cas12b | MCVSRLPWFNITLTGKLNRQRLNQMCVSRLPWFCTPKGQLAATPKTVVAQQENAMLAIIRDVHEAAPADLKTVAQRLEPGYFVTQFPKQQMTGDEARAEAERLFAACQKKFKELAEYEDGYRQCLDALGPNLSLPRLGRKPKGAYPYAVVFKLMPTNATWECFKRVTASLYKRAQKGVVSPVSADSIADVRTNDEPLFEYFTNLALVRPPGNKDRAVWFEFDLAAFIEAIKSPHQFFQDTIKREQAVAQIKAKLDAMDGQGRAASGEEDALPGFEGDDRITLLRELVTDTLGYLAEADASTSPGGKIEYSIQERTVRGFAEVKRRWRDLVEKGKATEDALLKVLAEEQTEHRDDFGSATLYRELAKPKFQPIWRDPGTQPWHADDPLRAWLEYRELGRELEDKQRPIRFTPVHPVHSPRFFIFPKKKGGGRFGTVHEPGQLRVMAGIVAQTQHGWEPVPVRITYAAPRLRRDQLRDDVETDLESRPWLQPMMQALGLPEPDTADFSNCRVTLQPSAPDDIQLTFPVDVSADKLTTAIGKAARWAKQFNLFPDGDNFYNASLRWPHEKKPSKPPVPWHEALDNFSVLAADLGQRCAGAFARLEVRANDDFAGKPSRFIGETPGKKWRAALVAAGMLRLPGEEQTVWRPGATGPNFHTELSGSRGRMARPHEADDTADLLRAFDCPEESLMPADWRTSLSFPEQNDKLLVAARRYQSRLARLHRWCWFLTDEKKRQTALDEIREAEDMPAADDPQLTDKLRALLLQKQAALPGLLVRLANRILPLRGRSWQWETHPDKADCHLLTQTGPALPDVWIRGQRGLSMQRIEQIEELRRRFQSLNQMQRREIGGKPPIRRDDSIPDCCPDLLDKLDQIKEQRANQAAHMILAEALGLRLAPPPADKRQLRASRDVHGQYVKSREPVDFIVIEDLSRYRSSQGRAPRENSRLMKWCHRAVRDKLRELCEPFGIPVVETPAAYSSRFCSRSGVAGFRAVEVGPGFDREFPWMMLKDREDEGEPVRQLILQVATLNQGRDGKPPRTLLAPLAGGPIFVPIVDKLNGADIQPALAQADINAAINLGLRAIADPRLWSIHPRCRTQRQGDQMLTREKRKFGETGQPLAVHRADGVKPDDTRNPNFFADISGSLPAWESATLDGQHLLSGRCLRSEIKKRQWQRCAEINDRRMNRWMKGE |
| 736 | Cas12b | MTELQTQRAYTLRLKGIDEKDQSWRDALWKTHEAVNKGAKVFGDWLLTLRGGLDHTLADAEIPGEKGKPDRAPTQEERKHRRILLALSWLSVESERGAPEEFIVATGKEPAATRNDKVIAALKDILRGRNLTEEKISEWTEVCTPSLSAAIREDAVWVNRSRAFDEAVKRIGSSLTREEVWDMLECFFGSRNAYLAPVKISEDESSDGEQEEKAKDLVQKAGQWLSSRFGTGEGADFAKMAAVYAKIAAWAGNAQAGTTGNEVINNLATALREFTPKSNDLKGVLDLISGPGYKSATRNLLKQIANTKTVTREDISKLQETAGEDSEECATKTGSKGKRAYADAILKDVESVCGFTYRIDKDGQPVSVADYSKYDEDYKWGSSRHKEFAVMLDHAARRVSLAHTWIKRAEAERRKFEEDSKKIMQVPQAAKDWLDAYCAQRSEASGALEPYRIRKRAIQGWKEIIASWNKPDCKTAEDRIAAARQLQDDPEIEKFGDIQLFEALAEDDAQCVWKKEDGTLDPEILINYTLASEAMFKKQHFKVPSYRHPDAFLYPVFCDFGNSRWELDFSIREAATKLKEIEAKIEKQRQEVHKVQQALEKCENDEKRPKMEERLKEAQKKLQESQNYGEYLHSNNKITMVLFDGTFVKKHIFAWQSKRLTKDLALYQEPSADPKNVVSRADRLGRAVASVGINDAVKVAGLFEQENWNGRLQAPRQQLEAIAQYVEKHGWDNKAEKMRASIKWFITFSAKLQSKGPWNEFARKHGLKEDPHYWPHAEKNENRTAHSRLILSRLPGLRVLSVDLGHRYAAACAVWEALGSEAFKKDIEGKRIIRGDTDENALYCHTEHEANGKKHITIYRRIGADTLPDGAHHPAPWARLDRQFLIKLQGEDEQAREASNEEIWKVHQLENTLGRRTPLIDRLIAGGWGYTEKQKARLEVLTNLGWCPTNKTDNQEEGDEEETAILSKPSLLVDDLMFSAVRTLRLALKRHGDRARIAHYLITDEKTKPGGVKEKLDKNGRVELLLDALGLWHDLFSSPGWHDEKAKQLWNAYIAGLLPEGELQQAKSVTTSAALGGQQKKEKKEKLRAVAEALYLNSDLCHSLNEVWRKRWEEDDKQWRIYIRWFKDWIMPRGANAKSPAIRHVGGLSLTRLATLTEFRRKVQVGFFTRLHPDGTKTETREDFGQKTLDTLEHLREQRVKQLASRIVEAALGIGSEDKRHWDGKKRPRQRIADPRFVPCHAVVIENLTHYRPEETRTRRENRQIMEWASSKVKKYLSEICQLHGLHLREVSAAYTSHQDSRTGAPGIRCQDVSLIEFMKSPFWRKQVAQAEKKQKEGKGDAVERYLCELNQKWKGASEEEWRKAGFVRIPLRGGEIFVSAAGHDSPAAKGIHADLNAAANIGLRALLDPDWSGKWWYVPCNSSTMCPARDKVTGSAAVNPGQPLQVSAQLESDDAAKDTKKRKKKGDGKSKEIINLWRDISSYPLEDTRGGTWSNKTVYWNRVQSNVVHILQNQMKG |
| 737 | Cas12b | MPETTQRAYTLRLQGHDPKDASWREALWKTHEAVNRGAKAFGDWLLTLRGGLDHSLADEGAPGQTPTEEQRKQRRILLALSWLSVESENGAPQEYIVPHDRDNESGARQNWKTREALREILKNRGCRDDEIESWCHDCEPSLTSAIRKDAVWVNRSKAFDNAVQSIPNFSREEIWDLLGCFFVSSQAYLAPLESPKDDKPDASKKDSSKDLIQSAGQWLSRRFGRGKGLNFARLAETYEAIARWASVANPGDTNDLIADLAKTLNAETPELDGILKVVSGPGHKSKTRNLLRSLSAVNHITKDTLQRLKDTANEDAKKAKLKKGEKGHRAYAYKVLEAVEDACGFTYLQEGDRAKHCEFAVMLDHAARRVSSLHTWIKRAEAERRRFEIDTKKKDQLPPSVKEWLDTYCQKRSKETGAVEPYRIRRGAIEGWKEIVEAWSKAGTTTAEDRKHEARRLPDNPHIDKSGDIKLFEDLALEDALPVWHANGDPNNPPDPQLLIDYVEGSEAEFKKRAFKVPTYCHPDPLVHPVFCDYGCSRWNVSFAIQPVKKQKLSSEEKLPAKGLLLDLLHGTAIRPVALRWQSKRFARDLALNTTDSSDKPNEVTRADRFGCALAKCPSSQKIRIRGLFEEKYWNGRLQAPRPELTALAKRVAKYGWDKKARKLRNSLNWFITFSANLRPSGPWEEYTKYAEKAFSSNASAKPSVSRGGFWVVHASPNKRGKMAQLRLCRLPELRVLSVDLGHRYAAACAVWETLSKSAFEQEIHERKILRGGTGPNDLFCHTQHDTNGQSKVTIYRRIGADTLPNGTPHPAPWARLDRQFLIKLPGEEREARKASPTELANVEKLEKELGLKTSENRVKRIDDLMSDTLRTVRQALRRHSLRARIAFNLATLRDQSDGDEESQSKQKRDTRWNNTVKIWHSLLESNEWTDDWAKALWDELGPLSDPQKADDAEWLKLAAEKFYTRWQEDEQTWRERLRWLRRWILPRGSQAASQKGSIRHVGGLSLTRLATIKTLYQVLKAYHMRLKPDNSRKNIPAEGDEALQNFGQKILDDLEHMREQRVKQLASRIVEAALGLGRMKQVTIGKDPKRPREPVDQSCHAVVIENLTHYRPEKRQTRRENRQLMDWSAAKVKKYLKECCQLHGLHLVEVSASYTSRQDSRTGAPGIRCQEVPLTDFLKKNFWREQVKQAKQRLSEGKANARDRYLCQLNERWGNAPAPVTQTAIRLRIPLNGGELFVSADQNSPASKGIQADLNAAANIGLRAITDPDWPGAWWYVPCEANTFKPVKDKVAGSAAIDSNVSLKKDSPNSEKPASDRKSRTSKSMINLWCDTSSKSLSEKDQWQESAPYWEDVAARTINILQASLACSTTNSQ |
| 738 | Cas12a | MKSLAQFQNLYALQKTLRFELKPEGHTRETFNRWLEEIEKEQASENENIVYQDLLRAKKYEKIKIILDEYHKDFIEQALAYANLTELEKYEELYRKSNRTSEEEEEFENTKESLRKQIANIFIKNPNKTVQERWKFLFSKKLIQNELIVWVKGNYELLSEKLKNEFPDESSIISTIEDFKYFTTYFRNYHENRKNLYSNEDKFSTIAHRLIHENLPKFIDNIAIYQKAKAVLNINEVEKELGLPEDTLDKIFSLDFFSKALTQKGIDQYNYFLGGKTENEVKKIKGLNEFINLYNQQQQDKNQRLPFLKVLYKLPLFERTSTSFRFEPIENDRDLIERIGKFYYNDLKQYRDDSQGDTTDILSGINTLLRHVHDYRDGLYVNGGITLTQISQKIFGSWSYINNALAYFYDTYIDASGVDHQGERKPKKQKQIQEKTKWLKQKQFPVILVEKALSEYKSIETNEDLKTRISDTTLCDFFKRCGNDDNGQDLFDRIEARLREKNEEGYSLEDLLKKEFTTERKLMQDKTKTLLIKNFLDVIQGDKDDITAGLLHFVKCLIPRTEISEKNELFYSGMEKYLNILSEVTPLYNKARNYLTQKPYSIEKVKLNFENSTLLDGWDENEESDNSCVLLRKRGYYYLGIMNKKHNMIFDRKIYPKATEGEAYYEKMIYKLLPGAYKMLPKVFFSEKNIDYFKPSEEILRIRNTASYSKNGQPQEGYQKASFSIEDCRKYIDFFKKCIANHWDWQKFNFNFSPTEYYQSIDEFYREIERQGYKIDFVKIPESYINQLIKENKLYLFKIYNKDFSEKKKSKGKDNLHTLYWKMLFDEKNLKDVVLKLNGEAEVFFRQKSILYNEEIWNKGHHYSELKDRFSYPIISNKRYAEDKFFLHVPITLNFKADGINNVNNMVNEFIKDNRDIHIIGIDRGERHLLYVSVINQKGDIVEQCSLNEIVTEYNGKIFKKNYHEELDNLEKERDRARKDWQTIANIKELKEGYLSHVIHKISKLILKYNAIVVMEDLNSGFKRGRQKVEKQVYQNFEKQLIEKLNYLVLKESNVDEPGGVLRAYQLANKFETFKKLGKQSGIIFYVPAAYTSAIDPVTGYIQYLYPLKQADSVEKARKFYSQFKRISYNPHKQWFEFSFDYNDFNIIYHGKSSWTICTTNTERYMWNRLLNNGHGGEELVYVTNELELLFGEYNIIYGDGKDIKQQITDVQDIDVDRTAKQFYKRINELLNLTLKLRHNNGKKGADEEDYILSPVEPYFDSRFESRKPSMQQTLPINADANGAFNIARKGLLLLERLNQLGVEEFEKTKKSNNKKTQWLPHELWVEYAQNHTRK |
| 739 | Cas12b | MAYQNGKEQPTVTNQRAYTLRLSGTNDQDSIWRNRLWHTHEAVNKGAKTFGDWLLTMRGGLCHTLAEADVPGKGNKPARHPTPQEIRSRRVVLALSWLSVESQHGAPERHLVSHDLDIATGERKNWKTVEALREILHGRCLCKELIDEWANDCRDSLSATIREDAVWVNRSKAFDLAAKKIGASLTREELWDFLQPFFANKHGYLQMDTVAGVTNGDSETDAEEAKEDSSEEKAKDLSQKAGQWLSSRFGTGTGADFSRFSKVYEVLAARCGSVAVGVSGVEAIRILAGTLADFSPGSNDIEGMLGLMSGPGYKSATRNILQKINTLQTVSQQDLDRLREASEKDALQSKQKVGGKGSRPYANAILQDVEAACGICYAGTGESPARHWQYAVILDHAARRVSMAHSWIKRAEEQRSKFEIEKDKLDHVPKDALAWLDAFCARRSSESGASDAYRIRRSAVDGWKQVVAAWAALPPKPENQGSELLSDAESARIQAARELQDTVEKFGDIQLFEALSLTGAKCVWQPDGRPDAQPLLDYVAGTDAISKKQRFKVPAYRHPDALLHPVFCDFGNSRWNINYAIHRAPEKLTPAQQLLEKKKAEIDKAELTLAKAGDAAKQANISEKINGLRAAFIQQQEKVAWLNSRHAMTMSLWDGTHIEDTPLIWQSKRFGSDIGQPVEAQPLPVSRADRFGRAVALAQDNVPVIPSGLFDLSDWNGRLQAPRRQLEAIAAIRDSAKLSVNEKQQLVAKRIQSIRWLLTFSAKLQSHGPFIAYAAQHGFDWRYGAHGPENKSRQGLAKLILCRLPGLRILSVDLGHRYAAACAVWETLNAGQIQKACLDAGKEAPGPCTLYLHLKQIANGKEKKTIFRRIAADTLPDGSPHPAPWARLDRQFLIKLQGEDRDARLATSEEIAAVEQMENELGVVRQLKRKGRELLVDELMSDALRTLRLGLRRHGVRARIAFNLTANKRIRPGGKEEVLDQEGRVLLLTETLLAWYELYTAERWTDEPARELWNRHIQPLLGATILQNTVNQEDTPSAAKRRKLREETSGKLKHVAEEIAKNDSLCRQLHVLWSAQWQTEDVIWRTRLRMMRRWLLPRGVKRNAQLRISIRDVGGLSLTRIASFKSLYQVQKAYQMRPHPEDPRLNIPERGDSRLENFGQRVLDAMERMRENRVKQLASRIAEAALGIGGETGISSKDGSQKKRPTERSSDPRFAPCHAVVIEDLTHYRPDETQTRRENRQLMSWSSSKVKKYLGEACELNGLYLREVSPAYTSRQDSRTGAPGLRCNDVTVVEFNNSPFWRKQVGAAEKNQKEGNKGDARERYLLSIEEGIRGAANDRDIFRIPVKGGEIFVSACITDGGNNAKKNAPPGLQADLNAAANIGLRAIFDPDWEGRWWYIPCDAATLCPDAKKFIGCKAVDPTKPLRVVAEEGAISASGIGSKKSGRKKNAATDGTRIVNLWRDPSGAPIHRDVLRSPEWQDYAGYWNEVQHRVIRNLKTCYEQTSQQEDPFVSQDADKPF |
| 740 | Cas12b | MKRLAETALADKVKCETNSRPKGERAYANSILHDVESACGFTYRVDKGEQPVPVSDYSHYANDYRWGPANHSEFAVMLDHAARRVSLAHTWIKRAEAERRQFEENAKKIDKVPKVAREWLDSLCAERSIVLGALEPYRIRRRAVDGWKHVVAAWSKSDCKTAQDRITAARLLQEDPEIDKFGDIQLFEALAEDHAVCVWQRDGEAGKTSDPQLLIDYALAAEAEFKKRHFKVPAYRHPEAFWHPVFCDFGQSRWKICFDVHKNRQSRRQRACANRISRKICFDVHKKRQTLRLSLEVWTGSKMLDMPLCWQCKRLARDLALGQDHKKDRSCQVTRADRLGRAVSNVARNQEVQILGLFEQEYWNGRLQAPRPQLEALGRYIEKHGWDAKAQKSCRAIRWMISFSPRLQPAGPWGKFAEKLQLNPNPKYWPHAEDNKDRGSRSKLILCRLPGLRVLSVDLGHRYAAACAVWEAVDAEQVKEACQAAGHREPNENDLYLHLKKRTTKQKKGSQGVVEETTIYRRIGADTLPDCTPHPAPWARLDRQFLIRLQGEEDEARAASNEEVWAVHKLEAELGRTIPLIDRLLGAGWGQTEKQKARLKALRELGWTPANKCQAFNSTDETELRRPSLAVDELMLDAVGTLRLALKRHGDRARIARYLITDERTKPGGVKEKLDENGRIELLQDALIIWHGLFSSPRWRDDAAKQLWNEHIAKLVGEQNLVEVSEDASGSERRTKQKQNREKLREAAKALVDDVALRQALHDMWKRRWEEEDREWRRRLRWFKDWVLPRREQARKAYSRPAETGSSSHPKRRARYAAIRRVGGLSLTRLATLTEFRRKVQVGFFTRLKPDGTKAEAKEGFGQSTLDALEHLRAQRVKQLASRIVEAALGVGRIRRFPGVKNPKRPDTPVDKPCHAIVIENLTHYRPEETRTRRENRQLMTWSSSKIKKYLAEACQLYGLHLREVTAAYTSRQDSRTGAPGLRCQDVPVKEFMRSLFWRKEVAQAEKKLTAGKGSSYERLLCELNQRWKDNSPGDGKRAELLRLPHKGGEIFVSAAPDSPAARGLQADLNAAANIGLRALTDPDWPGKWWHVPCNAVTFRPVEDKVKGSAAVKLDQSLRQVAHPQSKDPGAKKSKEIVNLWCDISSLPLEHREWKLDWEPYPAYWNNVQCRVIRVLQGKV |
| 741 | Cas12b | MANAKVKTTTRSYTLSLNAPSDTTDRSPLWHRIFRTHYAICCGAREFGKLLLDLRGGLPTSLAQLGEGIAENDRRQTQRGTRRILALGWLSVEDLDHARNDPHRVQDTAPGSPLDQDLAEKILRKILITKGIKSEEEQSNWISDCLPALTANIRPDAVWVNRAESFAQWQRGTQPGAQPPTPEEAQQILFSLCGESLVTLTLPEQPAAAGQKQPDQETSPDPEEQTDRPPAAPSADDEMDPSNASRGIFGDLFGENAEGKRSRSQGKDNFACAVRDFLCANPTPSADAITEFREKQKPREPNPPGPEKYPPEVSTSGAPTAVAKRYRKLLVCAGLWPKSADEDGSSRNSAKTKFADPKEPQKTEIQINALDLIDACNQAAPADDSGTSPKAGRVFAPAWASNIAEKVASATQMPANAKSLNEFKRLMFALAARRFSQTQSWTRRNEAERHMAAARQDAAVARLREIDPDHKAQDWLRGYEQRRADQSGSNGEFRITRRMIGEAEAVFKAWAGTNSAAERELKTVAVQTTAEKFGDAALYSEIARNTAAEAVWRSGSAPEILDQWVKLRKAQSDQQRTRVPRFCHPNAFRHPTWCEFGESSKPGVWYAWNPKSKPRKPEVGGEGDGTRRLWVLLPDFNSGIGQAVPLRWRSKRLSKDLGEALQPSDAPIPRADRVSIAAAGLNLEGANGVPARYRPSLPFSENTKGWNARLQANRTALLHLESKWDAEAATWRDGGRSLLALKWFTTFSPELAMSEGPGRAIHPKLGWNSEPHSDLNRAQKRGGNAKLILSRLPGLRVLSVDLGHRYAAACAVWETLTTEQMNAACQAKNHTQPAESDMYVHLAHPTERVVKSGRKKGQNLIQTTVYRRIAADTLPDGTPHPAPWGRLDRQFLIKLQGEQRPTRAASKNEADLANALFHRLGLRSDADSENKSRAVDKLMARTVRVATLGLKRHARRAKIAYALDPNTKAIPGMGGSSAAFTPGDEPHIHLLTDALFDWQSLATDAKWDDAHARSLWNHHIATLPGGFHLENPTPRDESAHEPSRQRQRSGDDALRATLKPIAEKLSKADRQEVHAAWKKYWGDSDGQSAIVPKVLQGQRGPEKTTPSASASGWHGKIRWITDWIMGKYLEGCTGHAWKHDVGGLSVSRITTMKSLYQLHKAFAMRATPEKPRGAPEKGESNLGAAQGILTAMESMRQQRVKQLASRIAEAALGAGIERRSDNGRELQRPRERVDDPRFAACHAVVVEDLTNYRPDEMQTRRENRQLMQWASSKVKKYLSEACQLHGLYLRGVPAGYTSRQDSRTGAPGVRCGDIPVEELMAAPRWRRQILTAEKTRRENNTGTARDRYILTLDEKYRLLTAEQRKKTPPARIPVKGGDLFVSADPDSPAASGIQADLNAAANIGLKALIDPDWPGRWWYIPCDATTHKPSPERTRGSAAVDCDVPLGPDSTGTPEDRDAKPKKNQRNSKIAGRGQSAIINLWRDPTHLPIKENPSAWCESKKYWNQVEHNVVKVIESKGQKLTQTAEAATGESASSPPIAPTDVPW |
本文教示，在一些實施例中，使用熱穩定Cas酶將為尤其期望或有用的。在一些實施例中，有用的熱穩定Cas蛋白係Cas12或Cas13同源物(例如直向同源物)。在一些實施例中，有用的熱穩定Cas蛋白係Cas酶，其包含與SEQ ID No. 1至71或530至741中之任一者具有80%、85%、90%、99%或100%序列一致性之胺基酸序列。
替代地或另外，在一些實施例中，有用的熱穩定Cas蛋白在高於約50 ℃之溫度下起作用(例如其附帶裂解活性充分發揮功能)；在一些實施例中，高於選自由以下組成之群組之溫度：約55 ℃、約56 ℃、約57 ℃、約58 ℃、約59 ℃、約60 ℃、約61 ℃、約62 ℃、約63 ℃、約64 ℃、約65 ℃、約66 ℃、約67 ℃、約68 ℃、約69 ℃、約70 ℃、約71 ℃、約72 ℃、約73 ℃、約74 ℃、約75 ℃、約76 ℃、約77 ℃、約78 ℃、約79 ℃、約80 ℃、約81 ℃、約82 ℃、約83 ℃、約84 ℃、約85 ℃、約86 ℃、約87 ℃、約88 ℃、約89 ℃、約90 ℃、約91 ℃、約92 ℃、約93 ℃、約94 ℃、約95 ℃、約96 ℃、約97 ℃、約98 ℃、約99 ℃、約100 ℃或其組合。在許多實施例中，有用的熱穩定Cas蛋白在高於約60 ℃之溫度下起作用(例如其附帶裂解活性充分發揮功能)。
在一些實施例中，有用的熱穩定Cas蛋白在實施核酸延伸及/或擴增反應之溫度範圍內起作用(例如其附帶裂解活性充分發揮功能)；熟習此項技術者非常熟悉各種此類反應及實施該等反應之溫度範圍。在一些實施例中，此一溫度範圍可高於選自由組成之群組之溫度：約60 ℃、約61 ℃、約62 ℃、約63 ℃、約64 ℃、65 ℃、約66 ℃、約67 ℃、約68 ℃、約69 ℃、約70 ℃、約71 ℃、約72 ℃、約73 ℃、約74 ℃、約75 ℃、約76 ℃、約77 ℃、約78 ℃、約79 ℃、約80 ℃、約81 ℃、約82 ℃、約83 ℃、約84 ℃、約85 ℃、約86 ℃、約87 ℃、約88 ℃、約89 ℃、約90 ℃、約91 ℃、約92 ℃、約93 ℃、約94 ℃、約95 ℃、約96 ℃、約97 ℃、約98 ℃、約99 ℃、約100 ℃或其組合。在一些實施例中，溫度範圍可為約60 ℃至約90 ℃。在一些實施例中，溫度範圍可為約60 ℃至約80 ℃。在一些實施例中，溫度範圍可為約60 ℃至約75 ℃。在一些實施例中，溫度範圍可為約65 ℃至約90 ℃。在一些實施例中，溫度範圍可為約60 ℃至約80 ℃。在一些實施例中，溫度範圍可為約60 ℃至約75 ℃。
本文另外教示，如本文所闡述之熱穩定Cas酶可尤其用於當及/或可允許在單一反應/容器中實施多個反應步驟(例如 用於「一鍋式」反應)時。因此，在一些實施例中，使用熱穩定Cas可減少或消除某些處理及/或轉移步驟。在一些實施例中，除核酸分離外之所有反應步驟皆可在單一容器(例如 以「一鍋式」格式)實施。
嚮導多核苷酸
在一些實施例中，本文提供識別並結合所關注目標核酸之嚮導多核苷酸。在一些實施例中，嚮導多核苷酸係嚮導RNA (gRNA、sgRNA)。在一些實施例中，本文之嚮導多核苷酸包含crRNA。在一些實施例中，crRNA與所關注目標核酸互補。
熟習此項技術者將意識到設計及鑑別所關注目標核酸之嚮導多核苷酸之多種方法。熟習此項技術者將意識到可用於設計所關注目標核酸之嚮導多核苷酸之多種算法及軟體。
本文使用基於可用SARS-CoV-2序列設計嚮導物之可用算法。本文闡述憑經驗鑑別可用於本文所述方法及組成物中之藉由現有算法表明之彼等嚮導多核苷酸(若有)之測試。如實例中進一步闡述，僅如本文中所述經特異性鑑別且憑經驗測試之彼等嚮導RNA可用於在本文所述基於CRISPR之偵測分析中偵測SARS-CoV-2。
在一些實施例中，嚮導多核苷酸與表23中所列之序列具有60%、70%、80%、90%、95%、90%序列一致性。在一些實施例中，嚮導多核苷酸包含表17中所揭示之crRNA。在一些實施例中，用於嚮導多核苷酸中之crRNA與表17中所列之crRNA具有60%、70%、80%、90%、95%、90%序列一致性。
LAMP
如上文所述，本文尤其提供某些LAMP技術及/或其組分，其具體有用性及/或有效性記載於本文中。在一些實施例中，如WO2000/028082；WO2001/034790；WO2001/077317；或WO2002/024902中所述實施擴增。
熟習此項技術者將意識到設計可用於LAMP中之引子之多種方法。本文闡述憑經驗鑑別可用於本文所述方法及組成物中之藉由現有算法表明之彼等LAMP引子(若有)之測試。如實例中進一步闡述，僅如本文中所述經特異性鑑別且憑經驗測試之彼等LAMP引子可用於在本文所述基於CRISPR之偵測分析中偵測SARS-CoV-2。
在一些實施例中，LAMP引子與表20中所列之序列具有60%、70%、80%、90%、95%、90%序列一致性，與表17中所列之引子序列具有60%、70%、80%、90%、95%、90%序列一致性。
經標記核酸報導子構築體
在一些實施例中，本文提供經標記核酸報導子構築體。根據本文，可藉由偵測經標記之適當核酸報導基因構築體之裂解來偵測CRISPR/Cas酶之裂解活性(例如附帶活性)。通常，用於本文中之經標記核酸報導子構築體之特徵在於可偵測到其裂解。熟習此項技術者意識到用於經標記核酸報導子構築體之多種策略及實施例，特定Cas酶對該等構築體之附帶裂解係可偵測的。僅給出一個實例，在一些實施例中，可用螢光發射染料對(例如FRET對或螢光/淬滅劑對)標記經標記核酸報導子構築體，使得在經標記核酸報導子構築體裂解時觀察到螢光變化(例如增加(例如在裂解減輕淬滅時)、減少、波長變化或其組合)。適當FRET對為此項技術中已知(參見例如Bajar等人，sensors (Basel)，2016；Abraham等人，PLoS One 10:e0134436，2015)。
用於偵測經標記核酸報導子構築體之裂解之多種其他策略亦為此項技術中已知，且包括例如如SHERLOCK™ (參見例如WO 2018/107129，其以引用方式併入本文中)所述遮蔽構築體。
樣品
在一些實施例中，本文之方法及組成物偵測樣品中之目標核酸。在一些實施例中，樣品係環境樣品。
在一些實施例中，樣品係生物樣品。在一些實施例中，生物樣品係自個體(例如人類或動物個體)收集。在一些實施例中，動物個體可為穿山甲、鳥或蝙蝠。在一些實施例中，動物個體可為家養動物，例如農場動物或寵物。在一些實施例中，動物個體可為貓、乳牛、犬、山羊、馬、駱馬、豬、綿羊等。在一些實施例中，動物個體可為齧齒類動物。在一些實施例中，個體可為靈長類動物。 在一些實施例中，個體可為人類。
在一些實施例中，生物樣品係自個體、例如自個體之流體或組織獲得。在一些實施例中，樣品係藉助拭子、抽吸物或灌洗自個體獲得。在一些實施例中，樣品係藉助鼻拭子、鼻咽拭子、口咽拭子、鼻抽吸物、痰、支氣管肺泡灌洗自個體獲得。
在一些實施例中，使用拭子收集之樣品係使用具有合成尖端(例如耐綸(nylon)或Dacron®)及鋁或塑膠軸之拭子來收集。在一些實施例中，不使用海藻酸鈣拭子。在一些實施例中，不使用具有木軸之棉拭子。在一些實施例中，將拭子立即放入含有2-3 ml病毒運輸培養基(即VTM、UTM、M4RT)之無菌管中。
在一些實施例中，樣品係經處理的。在一些實施例中，樣品係藉由稀釋、過濾、澄清、蒸餾、分開、分離及/或低溫保藏來處理。在一些實施例中，樣品係藉由分離特定組分來處理。在一些實施例中，樣品係藉由分離核酸來處理。在一些實施例中，自樣品分離RNA。在一些實施例中，自樣品分離DNA。在一些實施例中，核酸係使用管柱自樣品分離。在一些實施例中，在分離後在偵測目標核酸之前稀釋經分離核酸。在一些實施例中，在分離後在偵測目標核酸之前連續稀釋經分離核酸。
偵測極限
在一些實施例中，本文之方法及組成物提供目標核酸之靈敏性偵測。在一些實施例中，本文之方法及組成物可偵測3個、5個、7個、9個、11個、13個、15個、17個、19個、21個、23個、25個、27個、29個、31個、33個、35個、37個、39個、41個、43個、45個、47個、49個、51個、53個目標核酸病毒拷貝/µL所提取RNA。在一些實施例中，本文之方法及組成物可偵測3-11個、5-13個、7-15個、9-17個、11-19個、15-21個、19-23個、21-25個、23-27個、25-31個、27-33個、29-35個、31-37個、33-39個、37-41個、39-45個、45-49個、47-53個目標核酸病毒拷貝/µL所提取RNA。
格式
在一些實施例中，本文提供用於偵測SARS-CoV-2之尤其有用及/或有效的格式。
在一些實施例中，核酸分離可涉及例如細胞破壞、消化及/或去除非核酸細胞組分及/或核酸之沈澱。在一些實施例中，用於核酸分離之試劑可包括硫氰酸與胍之化合物(1:1)；蛋白酶K；熱；變性劑；清潔劑；載體RNA (例如酵母菌tRNA)。在一些實施例中，用於核酸分離之試劑可包括苯酚/氯仿；BHT；BHA；表面活性素；癸酸(8:0)；辛酸(10:0)；月桂酸；棕櫚油酸(16:1)；油酸(18:1)；亞麻油酸(18:2)；次亞麻油酸(18:3)；花生油酸(20:4)；二十二碳六烯酸(22:6)；三油酸甘油酯(Triolein)；單辛酸甘油酯(Monocaprylin)；單癸酸甘油酯(Monocaprin)；單月桂酸甘油酯(Monolaurin)；單油酸甘油酯(Monoolein)；單亞油酸甘油酯(Monolinolein)；單月桂酸甘油酯+BHA；單月桂酸甘油酯+山梨酸；癸醇；十二烷醇；L-精胺酸。
在一些實施例中，可在同一反應容器中實施靶擴增；CRISPR/Cas附帶活性活化；及信號偵測中之兩者或更多者。在一些實施例中，靶擴增；CRISPR/Cas附帶活性活化；及信號偵測皆係在同一反應容器中實施。
在一些實施例中， 靶擴增涉及環介導之等溫擴增(LAMP)。實際上，在一些實施例中，本文提供以下見解：相對於替代性擴增技術(例如基於核酸序列之擴增(NASBA)；股置換擴增；重組酶聚合酶擴增(RPA)；滾環式擴增(RCA))，LAMP提供某些意外的優點。
在一些實施例中，用於LAMP之試劑可包括例如緩衝液中之Bst 2.0WarmStart DNA聚合酶及WarmStart RTx反轉錄酶。
實例
實例 1. 一般 Sherlock SARS-CoV-2 分子診斷分析
本實例闡述本文所述之診斷及偵測分析之製備。使用標準去鹽純化自100 nmol規模合成獲得LAMP引子，且使用不含核酸酶之分子級水重懸浮至100 µM。製造10× LAMP引子混合物，然後運行分析。使用標準去鹽純化自2 nmol規模合成獲得crRNA，且使用不含核酸酶之分子級水重懸浮至1 µM。
在不含核酸酶之水中製備10× LAMP引子混合物。製備2 µM F3、2 µM B3、16 µM FIP、16 µM BIP、4 µM環F及4 µM環B之引子10×原液。在SARS-CoV-2 N之100 uL反應中，使用以下體積之所製備10×原液：2 µL之F3及B3、16 µL之FIP及BIP及4 µL之環F及環B，添加有56 µL水。在SARS-CoV-2 Orf1AB之100 μL反應中，使用以下體積之所製備10×原液：2 µL之F3及B3、16 µL之FIP及BIP及4 µL之環F，添加有60 µL水。在SARS-CoV-2 RNA酶P之100 μL反應中，使用以下體積之所製備10×原液：2 µL之F3及B3、16 µL之FIP及BIP及8 µL之環F及環B，添加有48 µL水。
藉由將310 μL不含RNA酶之水添加至310 μg凍乾載體RNA中來製備載體RNA，以獲得1 μg/μL載體RNA原液。製備洗滌緩衝液。 將60 mL之96%-100%乙醇添加至15 mL洗滌緩衝液(WII)濃縮物中。
製備溶解緩衝液。同時，使用使用下式計算處理樣品所需之溶解緩衝液之體積/載體RNA混合物： N × 0.21 mL (溶解緩衝液之體積/反應) = A mL，A mL × 28 µL/mL = B µL。其中N = 樣品數，A = 所計算溶解緩衝液之體積(L22)，且B = 所計算添加至溶解緩衝液(L22)中之1 µg/µL載體RNA原液之體積。向1 μg/μL載體RNA原液中添加載體RNA原液之體積(B，如上文計算)及溶解緩衝液之體積(A，如上文計算)。
製備溶解物。向微量離心管中之25 μL蛋白酶K中添加200 μL不含細胞之樣品(平衡至室溫)。向此管中添加200 μL溶解緩衝液(含有5.6 μg載體RNA)且藉由以速度10分之7至8渦旋15秒來混合。將管在乾熱塊中在56℃下培育15分鐘。遵循樣品溶解混合物管之脈衝離心以自蓋內部去除任何液滴。然後使管準備用於結合及洗滌步驟。
藉由以下方式結合及洗滌所製備RNA/DNA樣品：將250 μL 96%-100%乙醇添加至溶解物管中以獲得37%之最終乙醇濃度，然後以速度10分之7-8渦旋15秒。然後將管在室溫(19℃至26℃)下培育5分鐘。將管脈衝離心以自蓋內部去除任何液滴。將乙醇(約675 μL)中之溶解物轉移至旋轉管柱上，使該旋轉管柱經受約6800 × g離心1分鐘。將旋轉管柱置於潔淨的洗滌管中且將500 μL含有乙醇之洗滌緩衝液(WII)添加至旋轉管柱中並經受兩次約6800 × g離心1分鐘，在每次離心後丟棄收集管且丟棄流過物。藉由以＞13,000 × g離心來乾燥旋轉管柱。藉由將旋轉管柱置於潔淨的1.5-mL回收管中來完成RNA/DNA之溶析，且將30 μL不含RNA酶之無菌水添加至管柱中並在室溫下培育約1 min，然後使管經受13,000 × g離心1分鐘，溶析物含有經純化之病毒核酸。
藉由製備LAMP主混合物及含有期望引子之10×引子原液來實施使用LAMP最終體積為20 uL之擴增反應。向12 uL LAMP主混合物/引子原液中添加8 µL靶且混合，旋轉沈降。然後將樣品於設定為61℃之溫度循環器/加熱塊中放置40分鐘。
在37℃下在螢光微量板中以25 uL體積實施CRISPR-Cas偵測。藉由用25 µL不含核酸酶之水重懸浮個別管來製備2 µM RNA酶警報原液。製備Cas主混合物。Cas主混合物含有RNA酶警報(125 nM)、1mM rNTP混合物、T7 RNA聚合酶(1 U/µL)、 鼠類RNA酶抑制劑(1 U/µL)、LwaCas13a (6.33 ng/µL)、crRNA (SARS-CoV-2 N或SARS-CoV-2 Orf1AB或RNA酶P) (22.5 nM)及MgCl2 (9 mM)。
將20 µL之每一Cas混合物與5 µL擴增之LAMP樣品合併成8連管混合物，脈衝渦旋，且旋轉沈降。然後將LAMP-Cas混合物8連管之20 µL添加至384 Corning黑色透明底孔板中且密封。將板於37℃板中放置15分鐘且間隔1分鐘讀取。
在完成讀板儀運行後實施數據提取及分析且將數據輸出於excel表。對於陰性對照樣品，計算每一靶分析物之最終讀數(T15)對初始讀數(T0)之比率，且對於陽性對照樣品以及所有患者或人為樣品亦如此。
當將「無輸入RNA」反應設置為擴增之陰性對照時，對照定義為「陰性對照」。「陽性對照」係所提取病毒RNA，其以5000 cp/uL之濃度用作擴增及偵測每一SARS-CoV-2靶分析物之LAMP反應之模板。實施數據分析及結果解釋，使得若最終信號比有效「無輸入RNA」樣品高
5倍，則樣品視為陽性，且所有對照分析給出適當結果(定義於下文)。
實例 2. 偵測極限 (LoD) 之測定及確認 - 規劃。
| 結果 | 解釋 |
| 所有「陰性對照」樣品信號之初始讀數對最終讀數(即T0對T15)增加不到3倍 | 陰性對照結果係「有效的」且可使用陰性對照信號強度作為背景。測試運行係有效的 |
任一「陰性對照」樣品之初始讀數對最終讀數增加
 | 陰性對照結果係「無效的」；測試運行係無效的 |
| 在T15處，所有「陽性對照」樣品之信號比有效的「陰性對照」信號增加
| 測試運行係有效的 |
| 在T15處，任一「陽性對照」樣品之信號係＜「陰性對照」信號的5倍 | 測試運行係無效的 |
| 在T15處，對於一或兩種CoV目標，患者樣品信號係
| 樣品對COVID-19呈陽性 |
| 在T15處，對於兩種CoV目標，患者樣品信號係＜「陰性對照」信號的5倍，且RNA酶P信號係
| 樣品對COVID-19呈陰性 |
| 在T15處，對於兩種CoV目標，患者樣品信號係＜「陰性對照」信號的5倍，且RNA酶P信號係＜「陰性對照」信號的5倍 | 測試結果係無效的且應對樣品再測試。 |
本實例闡述用於測定SARS-CoV-2診斷之偵測極限之製劑。
研究中所用之SARS-CoV-2基因體RNA源自SARS-CoV-2之病毒培養物(分離物2019-nCoV/USA-WA1/2020，MN985325)，其係在草原猴(Cercopithecus aethiops)上皮腎細胞中繁殖且穩定於Trizol中。使用PureLink™病毒DNA/RNA微套組純化SARS-CoV-2基因體RNA且溶析於60 µL不含核酸酶之水中。經由兩個獨立數位PCR實驗量化所溶析RNA後，將濃縮之RNA稀釋至48,000 cp/µL，等分成一次性等分試樣，儲存在-80℃下且在使用之前立即解凍一次。將病毒RNA之此原液連續稀釋於水中以產生一系列濃度。
在此研究中使用陰性基質(NM)，其係彙集的鼻咽拭子基質，自32個有症狀之流感患者收集，藉由RT-qPCR使用CDC /紐約州衛生部(New York State Department of Health)引子探針組(方案LVD SOP-151.0)篩選，且確認對SARS-CoV-2 N1及N2靶呈陰性並對RNA酶P呈陽性。
產生LoD小組成員：在此研究中測試之每一樣品係藉由以下方式來產生：將10微升經量化之SARS-CoV-2基因體RNA (陽性)或水(陰性)添加至溶解處理之陰性基質中，以達成期望病毒濃度。在添加225微升之PureLink溶解緩衝液/蛋白酶K混合物且在56℃下培育15分鐘後，將10微升之病毒培養物(用於人為臨床陽性)或水(用於陰性)添加至200微升之陰性基質中。使用PureLink病毒RNA提取套組、遵循製造商之說明書提取此人為樣品，最終溶析體積為30微升。使用8微升之此溶析樣品作為CRISPR SARS-CoV-2分析靶向之每一分析物(即兩種SARS-CoV-2靶分析物及RNA酶P對照)之模板。
對照如下：1)提取對照：在SARS-CoV-2信號不存在下，RNA酶P偵測用作提取對照。2)陰性對照：設置「無輸入RNA」反應作為擴增之陰性對照並測定Cas反應之背景偵測水準。對欲測試之每一LAMP引子組及每一嚮導物實施此陰性對照。陰性對照係藉由用等體積之不含核酸酶之水替代LAMP反應中之8 ul模板體積來產生。3)陽性對照：對欲測試之每一Orf1AB及N LAMP引子組及每一Orf1AB及N嚮導物實施用於擴增及偵測SARS-CoV-2分析物之陽性對照。陽性對照係藉由用等體積之病毒RNA替代LAMP反應中之8 ul模板體積來產生，該病毒RNA係以不含核酸酶之水中5000拷貝/ul之濃度自上文所述之SARS-CoV-2病毒RNA原液材料提取。使用PureLink病毒RNA提取套組純化陽性對照。將最終RNA溶析於60 µL不含核酸酶之水中。藉由數位PCR量化經純化之病毒基因體RNA，且在不含核酸酶水中稀釋至5000拷貝/微升。將陽性對照等分試樣以一次性25微升等分試樣儲存在小於-70℃之溫度下且在使用前立即解凍一次。
使用彙集的鼻咽(NP)拭子基質實施分析型靈敏度(LoD)研究，該彙集的鼻咽拭子基質係在2019-2020年流感季節自有症狀之流感患者收集且藉由RT-qPCR分析使用CDC引子/探針組測定為SARS-CoV-2陰性。藉由以下方式測定LoD：使三名操作者對摻入彙集的NP拭子基質中之一系列7個濃度之經量化病毒材料及不含病毒材料之彙集的NP拭子基質運行SHERLOCK分析。 使用經測定對表現最差之靶之3/3個重複呈陽性之系列之最低濃度的20個重複來確認LoD。另外，由SHERLOCK操作者分析2×假定的LoD之20個重複以及單獨基質之20個重複。由4名操作者運行LoD確認。根據展示兩種靶(N及Orf1ab)之至少19/20個陽性重複之最低濃度來定義最終LoD。參見下表2。另外，若靶經測定在初始滴定中具有不同的推定LoD，則可獨立地確認(若期望)兩種靶，此將涉及產生經測定對表現最佳之靶之3/3個重複呈陽性之系列之最低濃度的額外20個重複。
對經處理用於下文所概述之LoD測定研究之每種樣品之NP拭子樣品實施Sherlock CRISPR SARS-CoV-2測試。
表2
LoD估計：將病毒培養物稀釋於不含核酸酶之水中，且然後在添加225微升之PureLink溶解緩衝液/蛋白酶K混合物且在56℃下培育15分鐘後摻入200微升NP基質中，以達成基於先前測試經預測為0×、0.25×、0.5×、1×、1.5×、2×、3×及5× LoD之濃度。由三名獨立操作者測試N=7個濃度中之每一者以及僅陰性基質樣品之N=3個重複。使用PureLink病毒RNA提取套組提取該等人為樣品，最終溶析體積為30 µL。使用8 µL之此溶析物作為偵測CRISPR SARS-CoV-2分析靶分析物(即N及Orf1AB)以及RNA酶P對照之每一SHERLOCK反應之模板。對每一CRISPR SARS-CoV-2分析靶分析物估計之LoD係偵測為對3個重複中之3個呈陽性之最低濃度。
樣品提取：提取樣品1-8。藉由下表3追蹤I期-LoD估計之樣品提取資訊。
表3
LAMP擴增：根據上文佈局使用下文用於反應設置之條形模板來實施LAMP反應。
| 樣品編號 | 小組成員ID | 描述 |
| 1 | [首字母縮寫]-[日期]-01 | 5× |
| 2 | [首字母縮寫]-[日期]-02 | 3× |
| 3 | [首字母縮寫]-[日期]-03 | 2× |
| 4 | [首字母縮寫]-[日期]-04 | 1.5× |
| 5 | [首字母縮寫]-[日期]-05 | 1× |
| 6 | [首字母縮寫]-[日期]-06 | 0.5× |
| 7 | [首字母縮寫]-[日期]-07 | 0.25× |
| 8 | [首字母縮寫]-[日期]-08 | 0× |
| 條中之位置 | |||||||||
| 條ID | 引子混合物 | 孔1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
| [首字母縮寫]-1 | Orf1AB | 樣品1 | 樣品2 | 樣品3 | 樣品4 | 樣品5 | 樣品6 | 樣品7 | 樣品8 |
| [首字母縮寫]-2 | N | 樣品1 | 樣品2 | 樣品3 | 樣品4 | 樣品5 | 樣品6 | 樣品7 | 樣品8 |
| [首字母縮寫]-3 | RNA酶P | 樣品1 | 樣品2 | 樣品3 | 樣品4 | 樣品5 | 樣品6 | 樣品7 | 樣品8 |
| [首字母縮寫]-4 | Orf1AB或N或RNA酶P | Orf1AB陽性對照 | N陽性對照 | ＜空白＞ | Orf1AB陰性對照 | N陰性對照 | RNA酶P陰性對照 | ＜空白＞ | ＜空白＞ |
對於每一所提取樣品，針對三個引子組中之每一者實施一次LAMP反應。另外，包括CoV目標之LAMP-Cas偵測之陽性對照(先前提取之5000 cp/µL之病毒RNA)。針對每一LAMP引子組及Cas反應實施含有水而非模板之LAMP-Cas之一次陰性對照。
I期LoD估計/ Cas偵測：遵循Sherlock CRISPR SARS-CoV-2測試說明書中所列之步驟設置且實施Cas反應。在384孔板中遵循以下模板實施反應。
| Cas嚮導RNA： | Orf1AB | N | RNA酶P | 如所指示 |
| LAMP條 | [首字母縮寫]-1 | [首字母縮寫]- 2 | [首字母縮寫]-3 | [首字母縮寫]-4 |
| 行/ 列 | 1 | 3 | 5 | 7 |
| A | 8 | 8 | 8 | ＜空白＞ |
| C | 7 | 7 | 7 | ＜空白＞ |
| E | 6 | 6 | 6 | RNA酶P陰性對照 |
| G | 5 | 5 | 5 | N陰性對照 |
| I | 4 | 4 | 4 | Orf1AB陰性對照 |
| K | 3 | 3 | 3 | ＜空白＞ |
| M | 2 | 2 | 2 | N陽性對照 |
| O | 1 | 1 | 1 | Orf1AB陽性對照 |
II期 - LoD確認：將所估計LoD (如自I期測試所測定)或2× LoD之20個重複摻入NM中。同時分析單獨基質之20個重複。使用Sherlock
TMCRISPR SARS-CoV-2測試針對兩種SARS-CoV-2目標分析物中之每一者以及RNA酶P測試每一提取。若每一SARS-CoV-2目標之
19/20個重複對SARS-CoV-2呈陽性，則稱已建立LoD。若＜19/20個重複呈陽性，則使用至少2×較高輸入之病毒RNA重複研究直至測定LoD。如上文所述之陽性對照及無模板陰性對照包括在所有LAMP運行中。
對於LoD確認樣品資訊，LAMP設置及Cas設置遵循下表之方案。
| 樣品編號 | 小組成員ID | 描述 |
| 101 | [首字母縮寫]-[日期]-101 | 1×LoD |
| 102 | [首字母縮寫]-[日期]-102 | 1×LoD |
| 103 | [首字母縮寫]-[日期]-103 | 1×LoD |
| 104 | [首字母縮寫]-[日期]-104 | 1×LoD |
| 105 | [首字母縮寫]-[日期]-105 | 1×LoD |
| 106 | [首字母縮寫]-[日期]-106 | 2×LoD |
| 107 | [首字母縮寫]-[日期]-107 | 2×LoD |
| 108 | [首字母縮寫]-[日期]-108 | 2×LoD |
| 109 | [首字母縮寫]-[日期]-109 | 2×LoD |
| 110 | [首字母縮寫]-[日期]-110 | 2×LoD |
| 111 | [首字母縮寫]-[日期]-111 | NTC |
| 112 | [首字母縮寫]-[日期]-112 | NTC |
| 113 | [首字母縮寫]-[日期]-113 | NTC |
| 114 | [首字母縮寫]-[日期]-114 | NTC |
| 115 | [首字母縮寫]-[日期]-115 | NTC |
| 條ID | 引子混合物 | 孔1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
| [首字母縮寫]-01 | Orf1AB | 樣品101 | 樣品102 | 樣品103 | 樣品104 | 樣品105 | ＜空白＞ | ＜空白＞ | ＜空白＞ |
| [首字母縮寫]-02 | Orf1AB | 樣品106 | 樣品107 | 樣品108 | 樣品109 | 樣品110 | ＜空白＞ | ＜空白＞ | ＜空白＞ |
| [首字母縮寫]-03 | Orf1AB | 樣品111 | 樣品112 | 樣品113 | 樣品114 | 樣品115 | ＜空白＞ | ＜空白＞ | ＜空白＞ |
| [首字母縮寫]-04 | Orf1AB | Orf1AB陽性對照 | Orf1AB陰性對照 | ＜空白＞ | ＜空白＞ | ＜空白＞ | ＜空白＞ | ＜空白＞ | ＜空白＞ |
| [首字母縮寫]-05 | N | 樣品101 | 樣品102 | 樣品103 | 樣品104 | 樣品105 | ＜空白＞ | ＜空白＞ | ＜空白＞ |
| [首字母縮寫]-06 | N | 樣品106 | 樣品107 | 樣品108 | 樣品109 | 樣品110 | ＜空白＞ | ＜空白＞ | ＜空白＞ |
| [首字母縮寫]-07 | N | 樣品111 | 樣品112 | 樣品113 | 樣品114 | 樣品115 | ＜空白＞ | ＜空白＞ | ＜空白＞ |
| [首字母縮寫]-08 | N | N陽性對照 | N陰性對照 | ＜空白＞ | ＜空白＞ | ＜空白＞ | ＜空白＞ | ＜空白＞ | ＜空白＞ |
| [首字母縮寫]-09 | RNA酶P | 樣品101 | 樣品102 | 樣品103 | 樣品104 | 樣品105 | ＜空白＞ | ＜空白＞ | ＜空白＞ |
| [首字母縮寫]-10 | RNA酶P | 樣品106 | 樣品107 | 樣品108 | 樣品109 | 樣品110 | ＜空白＞ | ＜空白＞ | ＜空白＞ |
| [首字母縮寫]-11 | RNA酶P | 樣品111 | 樣品112 | 樣品113 | 樣品114 | 樣品115 | ＜空白＞ | ＜空白＞ | ＜空白＞ |
| [首字母縮寫]-12 | RNA酶P | ＜空白＞ | RNA酶P陰性對照 | ＜空白＞ | ＜空白＞ | ＜空白＞ | ＜空白＞ | ＜空白＞ | ＜空白＞ |
統計/分析方法、樣本大小及驗收準則：
i) 在I期(LoD估計)中，對每一CRISPR SARS-CoV-2分析靶分析物估計之LoD係對3個重複中之3個呈陽性之最低濃度。
ii) 在II期(LoD確認)中，若出現以下情況則確認LoD：
● ≥每一CRISPR SARS-CoV-2分析靶分析物之19/20個重複對SARS-CoV-2偵測呈陽性，且
● 單獨基質之20/20個重複對SARS-CoV-2偵測呈陰性。
結果報導：
a) 陰性對照反應必須產生在15分鐘讀取過程中增加不到3倍(由T15/T0之比率定義)之Cas信號，陰性對照才能對靶有效。
b) 陽性對照必須對兩種CoV目標呈陽性；且陰性對照必須對所有三種靶呈陰性，分析運行才能有效。
c) 在陽性或陰性對照中之一或多者無法產生預期結果之情況下，必須重複受影響之分析運行。
d) 若Cas信號之T15讀數與靶之有效陰性對照(「未添加RNA」)反應相比增加
5倍，則樣品視為對該目標呈陽性。
e) 若在T15處，對於兩種CoV目標，患者樣品信號係＜陰性對照信號的5倍且RNA酶P信號係
陰性對照信號的5倍，則樣品對COVID-19呈陰性。
f) 若在T15處，對於兩種CoV目標，患者樣品信號係＜陰性對照信號的5倍且RNA酶P信號係＜陰性對照信號的5倍，則樣品係無效的。 必須自提取步驟開始重複此樣品。
實例 3. 偵測極限之測定及確認 1. 簡介
偵測極限(LoD)研究係分兩個時期實施。向彙集的鼻咽(NP)拭子樣品(在一步RT-qPCR實驗中使用CDC /紐約州衛生部引子及探針確認為對COVID19呈陰性)中摻入定量病毒SARS-CoV-2培養物或不含核酸酶之水，且然後使用Sherlock™ CRISPR SARS-CoV-2分析及套組來處理。
在I期(「LoD估計」)中，在陰性臨床基質存在下使用PureLink™病毒RNA/DNA微套組提取病毒SARS-CoV-2 RNA之限制性稀釋物之一式三份重複，且藉由Sherlock™ CRISPR SARS-CoV-2測試針對兩種SARS-CoV-2目標分析物(即ORF1ab及N)以及RNA酶P提取對照分析所提取RNA。ORF1ab之推定的LoD係4.5個拷貝/µL VTM且N之推定的LoD係0.9個拷貝/µL VTM。
在II期(「LoD確認」)中，對ORF1ab及N獨立地確認LoD。測試ORF1ab之推定的1× LoD濃度之二十(20)個重複樣品及1.5× LoD ORF1ab之20個重複。另外，如上文所述同時分析N之1× LoD濃度之二十(20)個重複及1.5×推定的LoD N之LoD濃度之20個重複。參見圖8。
ORF-1ab之LoD測定為6.75個拷貝/µL VTM且N之LoD測定為1.35個拷貝/µL VTM。Sherlock™ CRISPR SARS-CoV-02套組之LoD係6.75個拷貝/µL。
2. 測試方法：
a.測試目標：
陰性基質(NM)： 使用彙集的鼻咽拭子基質作為此研究之臨床基質，該彙集的鼻咽拭子基質係自53個有症狀之流感患者收集，在一步RT-qPCR方案中使用CDC /紐約州衛生部引子及探針(LVD SOP-151.0)篩選，且確認對SARS-CoV-2 N1及N2靶呈陰性並對RNA酶P呈陽性。
使用PureLink™病毒DNA/RNA微套組純化生長於Vero細胞株中之SARS-CoV-2之病毒培養物(穩定於Trizol中)之病毒基因體RNA，且溶析於60 µL中。經由兩個獨立數位PCR實驗量化所溶析RNA後，將濃縮之RNA於不含核酸酶水中稀釋至48,000 cp/µL。將病毒RNA之此原液連續稀釋於水中以產生一系列濃度。藉由在病毒稀釋物中摻入所溶解陰性基質(彙集的臨床鼻咽樣品)來生成人為陽性樣品。
對照：如LoD方案PRO-100-0004 Rev 01中所述，LAMP至Cas反應之每個實驗運行含有針對兩種CoV目標之陽性對照(將先前提取之4,800個拷貝/µL之病毒RNA直接添加至LAMP反應混合物中)以及陰性「未添加RNA」對照(NTC)。
b. 設備/儀器：
研究中所用之設備及儀器列於實例5中。
c. 方案步驟之彙總(彙總測試程序)：
LoD I期
● 測試0×、0.25×、0.5×、0.75×、1×、1.25×、1.5×、1.75×、2×、3×及5× LoD濃度之三個重複以估計LoD。
● 三名操作者各自對11個樣品實施核酸提取，且將經純化之RNA溶析於30 µL水中。
● 每一操作者製備三種2× RT-LAMP混合物(一種目標各一種混合物：ORF1ab、N、RNA酶P)，足以用於11個樣品及2個對照，且將12 µL等分至連管中。
● 操作者將8 uL所溶析RNA添加至相應連管中且將LAMP反應物在61℃下培育40分鐘。
● 每一操作者製備三種含有相應CRISPR嚮導RNA (crRNA)之CRISPR-Cas混合物(一種靶一種混合物：ORF1ab、N、RNA酶P)，且將20 µL等分至連管中。
● 操作者將Cas板密封並將其置於37℃讀板儀中，且以2.5分鐘間隔持續總共15分鐘收集相對螢光信號強度數據。
LoD II期
● 測試所估計1× N LoD (如自I期測試所測定)之20個重複樣品以及1× ORF1ab LoD之20個重複。另外，測試所估計N 1.5× LoD (來自1期測試)之20個重複及1.5× ORF1ab LoD之20個重複以確認兩種SARS-CoV-2靶(ORF1ab及N)之LoD。同時，針對ORF1ab及N二者測試0× LoD之20個重複。 獨立地確認ORF1ab及N之LoD，如下表4中所述。
● 五名操作者各自對20個樣品實施核酸提取，且將經純化之RNA溶析於30 µL水中。
● 每一操作者製備三種2× RT-LAMP混合物(一種靶一種混合物：ORF1ab、N、RNA酶P)，足以用於20個樣品及2個對照，且將12 µL等分至連管中。
● 操作者將8 uL所溶析RNA添加至相應連管中且將LAMP反應物在61℃下培育40分鐘。
● 每一操作者製備三種含有相應CRISPR嚮導RNA (crRNA)之CRISPR-Cas混合物(一種靶一種混合物：ORF1ab、N、RNA酶P)，且將20 µL等分至連管中。
● 操作者將5 uL完全LAMP反應物添加至相應Cas反應連管中，短暫混合，且將20 uL Cas-LAMP反應物轉移至384 Corning黑色透明底孔板。
● 操作者將Cas板密封並將其置於37℃讀板儀中，且以2.5分鐘間隔持續總共10分鐘或15分鐘收集相對螢光信號強度數據。
此方案偏差不會改變任一人為樣品或對照結果之解釋。
3. 分析及論述：
| II 期 LoD 樣品重複 | ||||
| 目標 | 病毒拷貝 (cp/µL溶析物RNA) | 病毒拷貝(cp/µL VTM) | LoD預估值 | 樣品數 |
| ORF1ab | 45 | 6.75 | 1.5× | 20 |
| ORF1ab | 30 | 4.5 | 1× | 20 |
| N | 9 | 1.35 | 1.5× | 20 |
| N | 6 | 0.9 | 1× | 20 |
| ORF1ab /N | 0 | 0 | 0× | 20 |
| 結果解釋偏差 | |||
| 結果 | 方案準則 | 研究準則 | |
| 有效陽性 |  |  | |
| 有效陰性 |  |  | |
| COVID-19陽性 |   |   | |
| COVID-19陰性 |    |    | |
| 無效 |    |    | |
| 其中， | N O R N spc O spc N ntc O ntc R ntc t | =N目標反應螢光 =Orf1ab目標反應螢光 =RNA酶P目標反應螢光 =N目標陽性對照反應螢光 =Orf1ab目標陽性對照反應螢光 =N目標陰性對照反應螢光 =Orf1ab目標陰性對照反應螢光 =RNA酶P目標陰性對照反應螢光 =反應時間 | |
1期LoD
LoD估計：表4至表6中個別重複結果之值代表在T10處所指示目標相對於無模板對照(NTC)之倍數增加。若該倍數增加大於或等於5，則樣品視為對目標呈陽性。LoD估計為3/3個重複樣品中偵測為對Sherlock™ CRISPR SARS-CoV-2分析及套組之目標分析物(例如N及ORF1ab)呈陽性之最低濃度。LoD估計係獨立地針對ORF1ab目標(表4)及N目標(表5)來測定。II期LoD確認中所用之所估計LoD濃度顯示於下表4 (ORF1ab)及下表5 (N)中。
| 表 4 ： ORF1AB LoD 估計之彙總 | ORF1AB | ||||
| 病毒拷貝 ( 拷貝 /µL 所提取 RNA) | LoD 預估值 | 重複 1 | 重複 2 | 重複 3 | 總陽性 |
| 120 | 5× LoD | 35.0 | 44.2 | 55.8 | 3/3 |
| 72 | 3× LoD | 28.7 | 41.2 | 59.4 | 3/3 |
| 48 | 2× LoD | 28.4 | 42.7 | 56.0 | 3/3 |
| 42 | 1.75× LoD | 35.9 | 43.9 | 59.4 | 3/3 |
| 36 | 1.5× LoD | 31.0 | 1.2 | 58.2 | 2/3 |
| 30 | 1.25× LoD | 41.6 | 44.2 | 57.7 | 3/3 |
| 24 | 1× LoD | 38.2 | 1.1 | 1.1 | 1/3 |
| 18 | 0.75× LoD | 36.8 | 36.5 | 65.8 | 3/3 |
| 12 | 0.5× LoD | 1.3 | 43.0 | 1.0 | 1/3 |
| 6 | 0.25× LoD | 1.1 | 42.9 | 1.0 | 1/3 |
| 0 | 0× LoD | 1.0 | 1.1 | 1.1 | 0/3 |
| n/a | 陽性對照 | 28.5 | 40.0 | 57.9 | 3/3 |
| n/a | 陰性對照 | 1.0 | 1.0 | 1.0 | 0/3 |
| 表 5 ： N LoD 估計 之彙總 | N | ||||
| 病毒拷貝 ( 拷貝 /µL 所提取 RNA) | LoD 預估值 | 重複 1 | 重複 2 | 重複 3 | 總陽性 |
| 120 | 5× LoD | 65.5 | 5.6 | 36.9 | 2/3 |
| 72 | 3× LoD | 66.0 | 19.0 | 38.2 | 3/3 |
| 48 | 2× LoD | 60.6 | 19.3 | 36.5 | 3/3 |
| 42 | 1.75× LoD | 57.4 | 17.7 | 37.2 | 3/3 |
| 36 | 1.5× LoD | 55.5 | 16.4 | 36.1 | 3/3 |
| 30 | 1.25× LoD | 55.4 | 18.8 | 38.5 | 3/3 |
| 24 | 1× LoD | 58.0 | 20.1 | 35.7 | 3/3 |
| 18 | 0.75× LoD | 49.0 | 21.7 | 42.6 | 3/3 |
| 12 | 0.5× LoD | 51.5 | 19.1 | 39.8 | 3/3 |
| 6 | 0.25× LoD | 50.1 | 18.8 | 35.3 | 3/3 |
| 0 | 0× LoD | 0.8 | 0.9 | 1.0 | 0/3 |
| n/a | 陽性對照 | 44.5 | 18.0 | 34.9 | 3/3 |
| n/a | 陰性對照 | 1.8 | 1.2 | 1.1 | 0/3 |
| 表 6 ： LoD 估計之 RNA 酶 P 提取對照彙總 | RNA 酶 P | ||||
| 病毒拷貝 ( 拷貝 /µL 所提取 RNA) | LoD 預估值 | 重複 1 | 重複 2 | 重複 3 | 總陽性 |
| 120 | 5× LoD | 22.2 | 29.8 | 32.9 | 3/3 |
| 72 | 3× LoD | 23.3 | 28.8 | 33.5 | 3/3 |
| 48 | 2× LoD | 24.3 | 25.2 | 30.4 | 3/3 |
| 42 | 1.75× LoD | 24.4 | 12.7 | 31.9 | 3/3 |
| 36 | 1.5× LoD | 24.1 | 24.6 | 30.0 | 3/3 |
| 30 | 1.25× LoD | 22.3 | 24.7 | 27.3 | 3/3 |
| 24 | 1× LoD | 28.7 | 22.7 | 30.5 | 3/3 |
| 18 | 0.75× LoD | 29.8 | 20.3 | 31.9 | 3/3 |
| 12 | 0.5× LoD | 26.1 | 22.9 | 33.0 | 3/3 |
| 6 | 0.25× LoD | 24.5 | 26.3 | 31.7 | 3/3 |
| 0 | 0× LoD | 20.5 | 24.9 | 29.8 | 3/3 |
| n/a | 陰性對照 | 1.1 | 1.3 | 1.3 | 0/3 |
在II期中，藉由運行各自20個重複檢查1× LoD ORF1ab (30個拷貝/µL所提取RNA)及1.5× LoD ORF1ab (45個拷貝/µL所提取RNA)以確認LoD。對於N目標，藉由運行各自20個重複檢查1× LoD (6個拷貝/ µL所提取RNA)及1.5× LoD N (9個拷貝/ µL所提取RNA)以確認LoD，下表7。當每一CRISPR SARS-CoV-2分析目標分析物之≥19/20個重複對SARS-CoV-2偵測呈陽性時確認LoD，下表8。
4. 結論及建議：當≥19/20個重複對ORF1ab及N目標呈陽性時滿足LoD確認之驗收準則。對Sherlock™ CRISPR SARS-CoV-2分析及套組之N靶分析物確認之LoD經測定為：
9個病毒拷貝/ µL所提取RNA
1.35個病毒拷貝/ µL VTM樣品
| 表 7. 1× 及 1.5× LoD (ORF1ab 及 N) 之結果 | 個別目標結果 - 陽性數 / 重複數 | ||||
| 病毒拷貝 ( 拷貝 /µL 所提取 RNA) | 病毒拷貝 ( 拷貝 /µL VTM) | LoD 預估值 | ORF1AB | N | RNA 酶 P |
| 45 (ORF1ab) 9 (N) | 6.75 (ORF1ab) 1.35 (N) | 1.5× | 19/20 | 20/20 | 20/20 |
| 30 (ORF1ab) 6 (N) | 4.5 (ORF1ab) 0.9 (N) | 1× | 17/20 | 17/20 | 20/20 |
| n/a | n/a | 陽性對照 | 5/5 | 5/5 | n/a |
| n/a | n/a | NTC | 0/5 | 0/5 | 0/5 |
| 表 8. LoD 之確認 | ||||||
| 靶 | 病毒拷貝 (cp/µL溶析物RNA) | 病毒拷貝(cp/µL VTM) | LoD預估值 | 樣品數 | 偵測數 | 偵測率(%) |
| ORF1ab | 45 | 6.75 | 1.5× | 20 | 19 | 95 |
| N | 9 | 1.35 | 1.5× | 20 | 20 | 100 |
對Sherlock™ CRISPR SARS-CoV-2分析及套組之ORF1ab目標分析物確認之LoD經測定為：
45個病毒拷貝/ µL所提取RNA
6.75個病毒拷貝/ µL VTM樣品
實例 4. 使用人為臨床樣品之分析之臨床評估 1. 簡介 ( 彙總之程序及結果 ) ：
在真實臨床樣品不存在下，遵循PRO-100-0027 Rev 02 (使用人為臨床樣品之Sherlock™ CRISPR SARS-CoV-2分析之臨床評估)中所指定之程序，對人為陽性及陰性樣品實施臨床評估。藉由CDC/紐約州衛生部RT-qPCR引子/探針組(LVD SOP-151.0)確認對COVID-19呈陰性之鼻咽拭子樣品係以原樣使用，或摻入所提取之定量SARS-CoV-2病毒RNA以分別產生人為陰性及陽性樣品。使用Sherlock CRISPR SARS-CoV-2測試處理跨越Sherlock CRISPR SARS-CoV-2分析及套組之orf1ab目標分析物之2×、3×及5× LoD之總共30個人為陽性樣品及30個人為陰性樣品，以測定測試之陽性一致性百分比(靈敏度)及陰性一致性百分比(特異性)。
2. 目的：
使用人為臨床樣本測定Sherlock™ CRISPR SARS-CoV-2測試之陽性一致性百分比(靈敏度)及陰性一致性百分比(特異性)效能。
3. 測試方法：
a.測試目標：
使用PureLink™病毒DNA/RNA微套組純化SARS-CoV-2之病毒培養物(穩定於Trizol中)之病毒基因體RNA，且溶析於60 µL不含核酸酶之水中。經由兩個獨立數位PCR實驗量化所溶析RNA後，將濃縮之RNA稀釋至48,000 cp/µL。將病毒RNA之此原液連續稀釋於水中以產生一系列濃度。
藉由將病毒稀釋物摻入所溶解鼻咽基質中來生成人為陽性樣品。使用不同的鼻咽拭子基質臨床樣本來產生此研究之人為臨床樣品。藉由RT-qPCR使用N1、N2及RNA酶P之CDC/紐約州衛生部RT-qPCR引子/探針組篩選所有臨床NP拭子樣品之SARS-CoV-2之存在。所用之所有NP拭子樣品經確認對SARS-CoV-2 N1及N2目標呈陰性且對RNA酶P呈陽性。
自獨特的NP拭子樣品取出人為「陰性」臨床樣品，藉由RT-qPCR使用N1、N2及RNA酶P之CDC/紐約州衛生部RT-qPCR引子/探針組(LVD SOP-151.0)篩選SARS-CoV-2之存在，並確認對SARS-CoV-2 N1及N2靶呈陰性且對RNA酶P呈陽性，並原樣用於此研究。
對照：如LoD方案PRO-100-0004 Rev 01中所述，LAMP至Cas反應之每個實驗運行含有針對兩種CoV目標之陽性對照(將先前提取之4,800個拷貝/µL之病毒RNA直接添加至LAMP反應混合物中)以及陰性「未添加RNA」對照(NTC)。
b. 設備/儀器：
研究中所用之設備及儀器列於下表9中。
| 名稱 | 製造商 | 型號 |
| 讀板儀 | BioTek | NEO2 |
| 溫度循環器 | Analytika | Biometra TRIO |
| 溫度循環器 | Analytika | Biometra TONE |
| 微量離心機 | Axygen | Axyspin R |
| 微量離心機 | Ohaus | FC5513 |
| 微量離心機 | Ohaus | FC5513 |
| 乾燥浴/加熱塊 | Corning | LSE |
方案步驟之彙總：
● 測試0×、2×、3×及5× (對應於0個、90個、135個及225個病毒拷貝/ µL所提取病毒RNA)之ORF1ab LoD濃度之樣品以測定靈敏度及特異性效能。
● 五名操作者各自對12個樣品實施核酸提取，且將經純化之RNA溶析於30 µL不含核酸酶之水中。
● 每一操作者製備三種2× RT-LAMP混合物(一種目標各一種混合物：orf1ab、N、RNA酶P)，足以用於12個樣品及2個對照，且將12 µL等分至8孔連管中。
● 操作者將8 µL所溶析RNA添加至相應8孔連管中且將LAMP反應物在61℃下培育40分鐘。
● 每一操作者製備三種含有相應CRISPR嚮導RNA (crRNA)之CRISPR-Cas混合物(一種目標各一種混合物：orf1ab、N、RNA酶P)，且將20 µL等分至8孔連管中。
● 操作者將Cas板密封並將其置於37℃讀板儀中，且以2.5分鐘間隔持續總共10分鐘或15分鐘收集在CRISPR複合物活化時由RNA酶警報之裂解引起之相對螢光信號強度數據。
此方案將樣品#10之分析自n=15分鐘時之以下變成：
n=10分鐘時之以下：
| 表10 ：結果解釋偏差 | |||
| 結果 | 方案準則 | 研究準則 | |
| 有效陽性 |  |  | |
| 有效陰性 |  |  | |
| COVID-19陽性 |   |   | |
| COVID-19陰性 |    |    | |
| 無效 |    |    | |
| 其中， | N O RP N spc O spc N ntc O ntc R ntc t | =N目標反應螢光 =Orf1ab目標反應螢光 =RNA酶P目標反應螢光 =N目標陽性對照反應螢光 =Orf1ab目標陽性對照反應螢光 =N目標陰性對照反應螢光 =Orf1ab目標陰性對照反應螢光 =RNA酶P目標陰性對照反應螢光 =反應時間 | |
此方案偏差產生樣品#10為真陰性而非弱假陽性之更準確之解釋。此方案偏差不會改變任何其他人為樣品或對照結果之解釋。
4. 詳細測試結果 ( 數據 )：
所有樣品之比率計算包括在下表11至13中。
5. 分析及論述：
| 表11 ：Orf1AB 目標螢光比率 | |||||||
| 樣品 | 病毒拷貝/µL 所提取RNA | 樣品類型 | 重複1 | 重複2 | 重複3 | 重複4 | 重複5 |
| 1 | 0 | NS | 0.9 | 1.0 | 0.6 | 1.0 | 0.9 |
| 2 | 0 | NS | 1.2 | 1.0 | 0.7 | 1.0 | 0.9 |
| 3 | 90 | 2× | 101.1 | 28.3 | 18.6 | 59.2 | 43.6 |
| 4 | 225 | 5× | 105.9 | 28.6 | 22.1 | 55.1 | 45.3 |
| 5 | 0 | NS | 1.2 | 1.0 | 1.0 | 1.1 | 0.9 |
| 6 | 0 | NS | 1.2 | 0.9 | 1.0 | 1.1 | 0.9 |
| 7 | 90 | 2× | 118.3 | 28.1 | 25.1 | 54.6 | 47.5 |
| 8 | 0 | NS | 1.0 | 0.9 | 1.0 | 1.1 | 0.9 |
| 9 | 135 | 3× | 106.2 | 33.4 | 27.3 | 52.5 | 50.8 |
| 10 | 0 | NS | 1.0 | 1.1 | 4.8 | 1.0 | 1.0 |
| 11 | 90 | 2× | 93.3 | 29.4 | 24.5 | 50.9 | 42.5 |
| 12 | 90 | 2× | 83.2 | 30.1 | 24.5 | 50.7 | 44.3 |
| 陽性對照 | 99.6 | 30.9 | 22.6 | 48.1 | 53.6 | ||
| 陰性對照 | 1.1 | 1.1 | 1.1 | 1.0 | 1.1 |
| 表12 ：N 標螢光比率 | |||||||
| 樣品 | 病毒拷貝/µL 所提取RNA | 樣品類型 | 重複1 | 重複2 | 重複3 | 重複4 | 重複5 |
| 1 | 0 | NS | 1.1 | 0.9 | 1.3 | 1.1 | 0.8 |
| 2 | 0 | NS | 1.2 | 0.9 | 1.5 | 1.0 | 0.9 |
| 3 | 90 | 2× | 69.1 | 45.4 | 71.3 | 49.1 | 20.4 |
| 4 | 225 | 5× | 62.6 | 44.0 | 55.6 | 36.0 | 23.0 |
| 5 | 0 | NS | 1.1 | 0.8 | 1.3 | 1.1 | 0.9 |
| 6 | 0 | NS | 1.3 | 0.8 | 1.4 | 1.2 | 0.9 |
| 7 | 90 | 2× | 72.3 | 42.4 | 72.0 | 44.9 | 23.6 |
| 8 | 0 | NS | 1.2 | 0.6 | 1.3 | 1.2 | 0.9 |
| 9 | 135 | 3× | 67.2 | 57.5 | 69.2 | 39.5 | 23.7 |
| 10 | 0 | NS | 1.5 | 1.0 | 1.2 | 1.1 | 1.0 |
| 11 | 90 | 2× | 62.9 | 44.1 | 65.9 | 34.0 | 22.2 |
| 12 | 90 | 2× | 67.2 | 47.6 | 54.8 | 36.2 | 22.2 |
| 陽性對照 | 61.5 | 45.6 | 70.2 | 30.7 | 20.9 | ||
| 陰性對照 | 1.3 | 1.6 | 1.0 | 1.1 | 1.2 |
| 表13 ：RNA 酶P 目標螢光比率 | |||||||
| 樣品 | 病毒拷貝/µL 所提取RNA | 樣品類型 | 重複1 | 重複2 | 重複3 | 重複4 | 重複5 |
| 1 | 0 | NS | 28.0 | 34.1 | 42.6 | 29.8 | 10.7 |
| 2 | 0 | NS | 29.2 | 29.1 | 47.2 | 26.1 | 10.8 |
| 3 | 90 | 2× | 29.2 | 24.0 | 41.7 | 1.0 | 10.6 |
| 4 | 225 | 5× | 26.6 | 25.4 | 41.3 | 22.5 | 11.1 |
| 5 | 0 | NS | 30.0 | 26.2 | 44.4 | 26.1 | 10.4 |
| 6 | 0 | NS | 27.2 | 24.8 | 47.7 | 24.0 | 10.6 |
| 7 | 90 | 2× | 30.6 | 24.6 | 55.1 | 26.9 | 10.5 |
| 8 | 0 | NS | 31.5 | 24.5 | 51.8 | 28.3 | 11.3 |
| 9 | 135 | 3× | 32.9 | 51.3 | 64.7 | 24.0 | 9.0 |
| 10 | 0 | NS | 31.5 | 49.1 | 59.3 | 28.4 | 10.8 |
| 11 | 90 | 2× | 25.7 | 41.7 | 33.7 | 23.7 | 7.6 |
| 12 | 90 | 2× | 22.6 | 41.5 | 29.4 | 25.5 | 8.1 |
| 陰性對照 | 1.3 | 1.3 | 1.3 | 1.3 | 1.0 |
表11至表13中個別人為樣品及對照之值代表在t=10分鐘時所指示靶反應之螢光與相應陰性對照反應之螢光的比率，代表t=10分鐘及t=0分鐘時陰性對照反應之螢光比率之陰性對照除外。結果解釋闡述於上表3中且彙總於下表14及表15中。使用Wilson得分區間來計算信賴區間(95%)。
6. 結論及建議：
| 表 14 ： Sherlock CRISPR SARS-CoV-2 測試與根據靶濃度之預期結果一致 | ||
| 樣品濃度 | 樣品數 | 一致 % (95% 信賴區間 ) |
| 5× LoD | 5 | 100% (NA*) |
| 3× LoD | 5 | 100% (NA*) |
| 2× LoD | 20 | 100% (83.9% - 100%) |
| 陰性樣本(NS) | 30 | 100% (88.6% - 100%) |
| NA*，未計算5或更小之樣本大小之信賴區間 |
| 表 15 ： Sherlock CRISPR SARS-CoV-2 測試之 PPA 及 NPA 之計算 | |||
| 人為參照樣品 | |||
| + | - | ||
| Sherlock CRISPR SARS-CoV-2 測試結果 | + | 30 | 0 |
| - | 0 | 30 | |
| 總計 | 30 | 30 | |
| 陽性一致性百分比 ( 靈敏度 ) = 100% 陰性一致性百分比 ( 特異性 ) = 100% |
滿足研究之驗收準則 - 特定而言，
● 2× LoD樣品之≥19/20個重複對SARS-CoV-2偵測呈陽性，
● 組合3×及5× LoD樣品之10/10個重複對SARS-CoV-2偵測呈陽性，且
● 陰性樣本(NS)樣品之30/30個重複對SARS-CoV-2偵測呈陰性
Sherlock SARS-CoV-2測試之陽性一致性百分比(靈敏度)係100%，且Sherlock SARS-CoV-2測試之陰性一致性百分比(特異性)係100%。
實例 5 設備及試劑。
本實例提供可用於實施Sherlock SARS-CoV-2測試之試劑及設備之清單。
表16 - 反應試劑之清單
表 17 ： LAMP 引子及 crRNA 序列之清單
* RNA酶P POP7 LAMP引子公開於Curtis 等人(2018) J Virol Methods. 255:91-97中
表 18 ：其他材料及供應之清單
表 19 ：設備 / 儀器之清單
實例 6 LAMP 引子及嚮導多核苷酸設計
| 試劑 | 供應商 | 目錄號 | 原液濃度 | 100 個反應所需之 µL |
| RNA酶P 10× LAMP 引子混合物 | Sherlock (參見第7.5.1部分) | n/a | 10× | 200 µL |
| SARS-CoV-2 N 10× LAMP 引子混合物 | Sherlock (參見第7.5.1部分) | n/a | 10× | 200 µL |
| SARS-CoV-2 ORF1AB 10× LAMP 引子混合物 | Sherlock (參見第7.5.1部分) | n/a | 10× | 200 µL |
| crRNA | IDT | n/a | 1000 nM | 56 µL |
| LwaCas13 酶 | IDT | n/a | 0.5 mg/mL | 32 µL |
| 2× WarmStart RT LAMP | NEB | E1700 | 2× | 1000 µL |
| T7 RNA pol | NEB | M0251 | 50 U/µL | 50 µL |
| 鼠類 RNA 酶抑制劑 | NEB | M0314 | 40 U/µL | 62.5 µL |
| MgCl 2 | ThermoFisher | AM9530G | 1 M | 23 µL |
| RNA 酶警報 | IDT | 11-04-03-03 | 2 µM | 156 µL |
| PureLink™ 病毒 RNA/DNA 微套組 | ThermoFisher | 12280050 | n/a | n/a |
| 目標 | 描述 | 序列 |
| orf1ab | orf1ab-F3 | TGAAAATAGGACCTGAGCG (SEQ ID NO. 72) |
| orf1ab-B3 | ACACCTAGTCATGATTGCA (SEQ ID NO. 73) | |
| orf1ab-FIP | CCAATAGAATGATGCCAACAGGCGATAGACGTGCCACATGC (SEQ ID NO. 74) | |
| orf1ab-BIP | GATTGATGTTCAACAATGGGGTTTCATTACCATGGACTTGACAAT (SEQ ID NO. 75) | |
| orf1ab-LF-T7 | gaaatTAATACGACTCACTATAGGGAAGTGTCTGAAGCAGTGGAAAA (SEQ ID NO. 76) | |
| crRNA | gatttagactaccccaaaaacgaaggggactaaaacGATCATGGTTGCTTTGTAGGTTACCTGT (SEQ ID NO. 77) | |
| N | N-5F3 | GCTTCTACGCAGAAGGGA (SEQ ID NO. 78) |
| N-5B3 | GTGACAGTTTGGCCTTGT (SEQ ID NO. 79) | |
| N-5FIP | TACTGCTGCCTGGAGTTGAATTCCTCTTCTCGTTCCTCATC (SEQ ID NO. 80) | |
| N-5BIP | GCTTTGCTGCTGCTTGACAGTGTTGTTGGCCTTTACCA (SEQ ID NO. 81) | |
| N-5LB | ATTGAACCAGCTTGAGAGCAAA (SEQ ID NO. 82) | |
| N-5LF-T7 | gaaatTAATACGACTCACTATAGGGCTTGAACTGTTGCGACTACGT (SEQ ID NO. 83) | |
| crRNA | gatttagactaccccaaaaacgaaggggactaaaacGGTGATGCTGCTCTTGCTTTGCTGCTGC (SEQ ID NO. 84) | |
| RNA 酶 P POP7* | RP- F3 | TTGATGAGCTGGAGCCA(SEQ ID NO. 85) |
| RP-B3 | CACCCTCAATGCAGAGTC(SEQ ID NO. 86) | |
| RP-FIP | GTGTGACCCTGAAGACTCGGTTTTAGCCACTGACTCGGATC(SEQ ID NO. 87) | |
| RP-BIP | CCTCCGTGATATGGCTCTTCGTTTTTTTCTTACATGGCTCTGGTC(SEQ ID NO. 88) | |
| RP-LF | ATGTGGATGGCTGAGTTGTT(SEQ ID NO. 89) | |
| RP-LB-T7 | GAATTAATACGACTCACTATAGGGCATGCTGAGTACTGGACCTC(SEQ ID NO. 90) | |
| crRNA | gatttagactaccccaaaaacgaaggggactaaaacAGTGGAGGAGTGTCTTTTCAATTACTTG(SEQ ID NO. 91) |
| 名稱 |
| 0.2 mL連管 |
| 1.5 mL彈扣蓋管 |
| 分子級水(不含DNA酶/RNA酶) |
| 過濾器移液管尖 |
| 384 Corning黑色透明底低體積板 |
| 板光學密封件 |
| 自臨床樣品/合成RNA靶提取之RNA |
| 血清移液管 |
| 項目 | 製造商 | 型號 | SHLK# |
| 微量離心機 | Axygen | Axyspin R | SHLK-0031 |
| 微量離心機 | Ohaus | ----------------------- | SHLK-0033 |
| PCR工作站-通風櫃（Dead Air Box） | Air Clean | AC632DBC | SHLK-0034 |
| PCR工作站 | Air Clean | AC648DBC | SHLK-0035 |
| PCR工作站 | Air Clean | AC648DBC | SHLK-0036 |
| PCR工作站 | Air Clean | AC648DBC | SHLK-0037 |
| PCR工作站-通風櫃 | Air Clean | AC632DBC | SHLK-0038 |
| PCR工作站-通風櫃 | Air Clean | AC632DBC | SHLK-0039 |
| PCR工作站 | Air Clean | AC648DBC | SHLK-0040 |
| PCR工作站-通風櫃 | Air Clean | AC632DBC | SHLK-0041 |
| 讀板儀 | BioTek | NEO2 | SHLK-0045 |
| 讀板儀 | BioTek | NEO2 | SHLK-0046 |
| 乾浴/加熱塊 | Corning | LSE | SHLK-0047 |
| PCR工作站-通風櫃 | Air Clean | AC632DBC | SHLK-0051 |
| PCR工作站 | Air Clean | AC648DBC | SHLK-0052 |
| PCR機器(3 × 48孔) | Analytika | Biometra TRIO | SHLK-0056 |
| 生物安全櫃II類A2 4ft型 | Baker | Sterilgard III | SHLK-0057 |
| 生物安全櫃II類A2 4ft型 | PHCBI | NHE-N4002A | SHLK-0058 |
| 生物安全櫃II類A2 4ft型 | PHCBI | NHE-N4002A | SHLK-0059 |
| PCR工作站 | Air Clean | AC648DBC | SHLK-0060 |
| PCR工作站-通風櫃 | Air Clean | AC632DBC | SHLK-0061 |
| PCR工作站 | Air Clean | AC648DBC | SHLK-0062 |
| 讀板儀 | BioTek | NEO2 | SHLK-0063 |
| PCR機器(3 × 48孔) | Analytika | Biometra TRIO | SHLK-0067 |
| PCR機器(1× 96孔) | Analytika | Biometra TONE | SHLK-0068 |
| 台式離心機 | Beckman | Avanti J15 | SHLK-0069 |
| 生物安全櫃II類A2 6ft型 | NuAire | NU-425-600 | SHLK-0070 |
| 生物安全櫃II類A2 4ft型 | NuAire | NU-425-400 | SHLK-0071 |
| PCR機器(1× 96孔) | Analytika | Biometra TONE | SHLK-0072 |
| 微量離心機 | Ohaus | FC5513 | SHLK-0073 |
| RODI水純化系統 | EMD-Millipore | Milli-Q | SHLK-0075 |
| 渦旋機 | Corning | 6775 | SHLK-0086 |
| 渦旋機 | Ohaus | VXMNAL | SHLK-0087 |
| 迷你離心機 | Ohaus | FC5306 | SHLK-0088 |
| 迷你離心機 | Ohaus | FC5306 | SHLK-0089 |
| 迷你離心機 | Ohaus | FC5306 | SHLK-0090 |
| 迷你離心機 | Ohaus | FC5306 | SHLK-0091 |
| 迷你離心機 | Ohaus | FC5306 | SHLK-0092 |
| 迷你離心機 | Ohaus | FC5306 | SHLK-0093 |
| 迷你離心機 | Ohaus | FC5306 | SHLK-0094 |
| 渦旋機 | Ohaus | VXMNAL | SHLK-0095 |
| 渦旋機 | Ohaus | VXMNAL | SHLK-0096 |
| 渦旋機 | Ohaus | VXMNAL | SHLK-0097 |
| 渦旋機 | Ohaus | VXMNAL | SHLK-0098 |
| 渦旋機 | Ohaus | VXMNAL | SHLK-0099 |
| 渦旋機 | Ohaus | VXMNAL | SHLK-0100 |
| 渦旋機 | Corning | 6775 | SHLK-0101 |
| 渦旋機 | Corning | 6775 | SHLK-0102 |
| 渦旋機 | Corning | 6775 | SHLK-0103 |
| 渦旋機 | Corning | 6775 | SHLK-0104 |
| 迷你離心機 | Corning | 6770 | SHLK-0105 |
| 迷你離心機 | Corning | 6770 | SHLK-0106 |
| 迷你離心機 | Corning | 6770 | SHLK-0107 |
| 渦旋機 | Ohaus | VXMNAL | SHLK-0115 |
| 迷你離心機 | Ohaus | FC5306 | SHLK-0116 |
| 加熱塊 | Fisher | 14955218 | SHLK-0117 |
| 加熱塊 | Fisher | 14955218 | SHLK-0118 |
| 單通道移液管，P3 (各5) | Sartorius | -------- | -------- |
| 單通道移液管，P10 (各5) | Sartorius | -------- | -------- |
| 單通道移液管，P20 (各5) | Sartorius | -------- | -------- |
| 單通道移液管，P100 (各5) | Sartorius | -------- | -------- |
| 單通道移液管，P200 (各5) | Sartorius | -------- | -------- |
| 單通道移液管，P300 (各5) | Sartorius | -------- | -------- |
| 單通道移液管，P1000 (各5) | Sartorius | -------- | -------- |
| 多通道移液管M100 (各2) | Sartorius | -------- | -------- |
| 多通道移液管M10 (各2) | Sartorius | -------- | -------- |
| 電動移液管E10 (各4) | Sartorius | -------- | -------- |
| 電動移液管E100 (各4) | Sartorius | -------- | -------- |
| 電動移液管E300 (各4) | Sartorius | -------- | -------- |
| 電動移液管E1000 (各4) | Sartorius | -------- | -------- |
| 血清移液管 |
本實例闡述選擇LAMP引子及嚮導多核苷酸用於Sherlock SARS-CoV-2測試之製程。
使用LAMP引子設計軟體(例如PrimerExplorer)來設計擴增SARS-CoV-2之部分之LAMP引子。設計覆蓋SARS-CoV-2基因體內之多個靶之80多個引子組。LAMP引子經設計以生成覆蓋SARS-CoV-2基因體中之幾乎每個開放閱讀框之擴增子，包括目前用於基於PCR之SARS-CoV-2診斷或偵測系統中之彼等擴增子。
所生成LAMP引子之序列顯示於表20中
表20
| 名稱 | 序列 | 備註 | SEQ ID NO. |
| XL-500 | CAACGTGTTGTAGCTTGTC | b1-F3 | 92 |
| XL-501 | ACCATCAGTAGATAAAAGTGCA | b1-B3 | 93 |
| XL-502 | GAACCGCCACACATGACCATCTATAGATTAGCTAATGAGTGTGCT | b1-FIP | 94 |
| XL-503 | GGTGGAACCTCATCAGGAGATGTAACATTGGCCGTGACAG | b1-BIP | 95 |
| XL-504 | CCACAACTGCTTATGCTAATAGTGT | b1-LB | 96 |
| XL-505 | CGTTTCTATAGATTAGCTAATGAGT | b2-F3 | 97 |
| XL-506 | GGCAATTTTGTTACCATCAGT | b2-B3 | 98 |
| XL-507 | GAGGTTCCACCTGGTTTAACATATAGCTCAAGTATTGAGTGAAATGG | b2-FIP | 99 |
| XL-508 | CAGGAGATGCCACAACTGCTTATAGATAAAAGTGCATTAACATTGG | b2-BIP | 100 |
| XL-509 | TAACATTTGTCAAGCTGTCACGG | b2-LB | 101 |
| XL-510 | ATGGCCTCACTTGTTCTT | b3-F3 | 102 |
| XL-511 | TAACATTGGCCGTGACAG | b3-B3 | 103 |
| XL-512 | ACTTGAGCACACTCATTAGCTAATCGCTCGCAAACATACAACG | b3-FIP | 104 |
| XL-513 | GTCATGTGTGGCGGTTCACTACACTATTAGCATAAGCAGTTG | b3-BIP | 105 |
| XL-514 | ACGGTGTGACAAGCTACAACA | b3-LF | 106 |
| XL-515 | ATGTTAAACCAGGTGGAACCTCATC | b3-LB | 107 |
| XL-516 | GCTCGCAAACATACAACG | b4-F3 | 108 |
| XL-517 | GCATTAACATTGGCCGTG | b4-B3 | 109 |
| XL-518 | ACCATTTCACTCAATACTTGAGCAGTAGCTTGTCACACCGTT | b4-FIP | 110 |
| XL-519 | ATATGTTAAACCAGGTGGAACCTCGCTTGACAAATGTTAAAAACACT | b4-BIP | 111 |
| XL-520 | TCAGGAGATGCCACAACTGCTTAT | b4-LB | 112 |
| XL-521 | CCTAACATGCTTAGAATTATGGC | b5-F3 | 113 |
| XL-522 | TAACATTGGCCGTGACAG | b5-B3 | 114 |
| XL-523 | ACTTGAGCACACTCATTAGCTAATCCACTTGTTCTTGCTCGCA | b5-FIP | 115 |
| XL-524 | GTCATGTGTGGCGGTTCACTCACTATTAGCATAAGCAGTTGT | b5-BIP | 116 |
| XL-525 | GACAAGCTACAACACGTTGTATGTT | b5-LF | 117 |
| XL-526 | TAAACCAGGTGGAACCTCATCA | b5-LB | 118 |
| XL-527 | TGTTCTTGCTCGCAAACA | b6-F3 | 119 |
| XL-528 | TAACATTGGCCGTGACAG | b6-B3 | 120 |
| XL-529 | ACTCAATACTTGAGCACACTCATTATACAACGTGTTGTAGCTTGTC | b6-FIP | 121 |
| XL-530 | ATGGTCATGTGTGGCGGTTCCTATTAGCATAAGCAGTTGTGG | b6-BIP | 122 |
| XL-531 | GTTAAACCAGGTGGAACCTCATC | b6-LB | 123 |
| XL-532 | CAACGTGTTGTAGCTTGTC | b7-F3 | 124 |
| XL-533 | ACCATCAGTAGATAAAAGTGCA | b7-B3 | 125 |
| XL-534 | GGTTTAACATATAGTGAACCGCCACGCTAATGAGTGTGCTCAAGT | b7-FIP | 126 |
| XL-535 | GGTGGAACCTCATCAGGAGATGTAACATTGGCCGTGACAG | b7-BIP | 127 |
| XL-536 | CCACAACTGCTTATGCTAATAGTGT | b7-LB | 128 |
| XL-537 | GCTAATGAGTGTGCTCAAGT | b8-F3 | 129 |
| XL-538 | ACTTATCGGCAATTTTGTTACC | b8-B3 | 130 |
| XL-539 | CCTGATGAGGTTCCACCTGGTGAGTGAAATGGTCATGTGT | b8-FIP | 131 |
| XL-540 | GATGCCACAACTGCTTATGCTGTAGATAAAAGTGCATTAACATTGG | b8-BIP | 132 |
| XL-541 | TAACATTTGTCAAGCTGTCACGG | b8-LB | 133 |
| XL-542 | CATGGCTTTGAGTTGACATC | hk1-F3 | 134 |
| XL-543 | ACCTGTAAAACCCCATTGT | hk1-B3 | 135 |
| XL-544 | ATGTGGCACGTCTATCACATAGATGAAGTATTTTGTGAAAATAGGACC | hk1-FIP | 136 |
| XL-545 | TCCACTGCTTCAGACACTTATGCTGAACATCAATCATAAACGGATT | hk1-BIP | 137 |
| XL-546 | TGATGTTCAACAATGGGGT | hk2-F3 | 138 |
| XL-547 | TTAATCTTCAGTTCATCACCAA | hk2-B3 | 139 |
| XL-548 | GCTACATGTGCATTACCATGGACTTTTACAGGTAACCTACAAAGCAA | hk2-FIP | 140 |
| XL-549 | AGTTGTGATGCAATCATGACTAGGTTTATAGGATATTCAATAGTCCAGTC | hk2-BIP | 141 |
| XL-550 | CCACGAGTGCTTTGTTAAGCG | hk2-LB | 142 |
| XL-551 | CCATGATCTGTATTGTCAAGTC | hk3-F3 | 143 |
| XL-552 | GAACTGGGAATTTGTCTGC | hk3-B3 | 144 |
| XL-553 | CGTGGACAGCTAGACACCTAGCATGGTAATGCACATGTAGC | hk3-FIP | 145 |
| XL-554 | GCGTGTTGACTGGACTATTGAATATAACCATGTGTTGAACCTTTC | hk3-BIP | 146 |
| XL-555 | ATGAACTGAAGATTAATGCGGCTTG | hk3-LB | 147 |
| XL-556 | CATCATTCTATTGGATTTGATTACG | hk4-F3 | 148 |
| XL-557 | TCAATAGTCCAGTCAACACG | hk4-B3 | 149 |
| XL-558 | AGATCATGGTTGCTTTGTAGGTTACAATCCGTTTATGATTGATGTTCA | hk4-FIP | 150 |
| XL-559 | GTCAAGTCCATGGTAATGCACATCTTAACAAAGCACTCGTGG | hk4-BIP | 151 |
| XL-560 | TGTGATGCAATCATGACTAGGTGT | hk4-LB | 152 |
| XL-561 | GTCTATAATCCGTTTATGATTGATG | hk5-F3 | 153 |
| XL-562 | CCGCATTAATCTTCAGTTCAT | hk5-B3 | 154 |
| XL-563 | TACATGTGCATTACCATGGACTTGAAACAATGGGGTTTTACAGGTA | hk5-FIP | 155 |
| XL-564 | AGTTGTGATGCAATCATGACTAGGTTTATAGGATATTCAATAGTCCAGTC | hk5-BIP | 156 |
| XL-565 | CAGATCATGGTTGCTTTGTAGGT | hk5-LF | 157 |
| XL-566 | AGCTGTCCACGAGTGCTTT | hk5-LB | 158 |
| XL-567 | ACACTTATGCCTGTTGGC | hk6-F3 | 159 |
| XL-568 | ACACGCTTAACAAAGCACT | hk6-B3 | 160 |
| XL-569 | AGGTTACCTGTAAAACCCCATTGTGATTTGATTACGTCTATAATCCGT | hk6-FIP | 161 |
| XL-570 | AAGCAACCATGATCTGTATTGTCATAGACACCTAGTCATGATTGC | hk6-BIP | 162 |
| XL-571 | TGGTAATGCACATGTAGCTAGTTGT | hk6-LB | 163 |
| XL-572 | TGAAAATAGGACCTGAGCG | hk7-F3 | 164 |
| XL-573 | ACACCTAGTCATGATTGCA | hk7-B3 | 165 |
| XL-574 | CCAATAGAATGATGCCAACAGGCGATAGACGTGCCACATGC | hk7-FIP | 166 |
| XL-575 | GATTGATGTTCAACAATGGGGTTTCATTACCATGGACTTGACAAT | hk7-BIP | 167 |
| XL-576 | AAGTGTCTGAAGCAGTGGAAAA | hk7-LF | 168 |
| XL-577 | GGTAACCTACAAAGCAACCATGAT | hk7-LB | 169 |
| XL-578 | AGTCCATGGTAATGCACAT | hk8-F3 | 170 |
| XL-579 | GGGTTACCAATGTCGTGAA | hk8-B3 | 171 |
| XL-580 | GCTTAACAAAGCACTCGTGGAGTAGCTAGTTGTGATGCAATC | hk8-FIP | 172 |
| XL-581 | GATGAACTGAAGATTAATGCGGCTTGAACTGGGAATTTGTCTGC | hk8-BIP | 173 |
| XL-582 | TCAACACATGGTTGTTAAAGCTGCA | hk8-LB | 174 |
| MKW0112 | ATCAGAGGCACGTCAACATC | JP1F3 | 175 |
| MKW0113 | TCACCACTACGACCGTACTG | JP1B3 | 176 |
| MKW0114 | AGGGCTGTTCAAGTTGAGGCAAAGATGGCACTTGTGGCTTAG | JP1FIP | 177 |
| MKW0115 | ACGTTCGGATGCTCGAACTGCATGCCTTCGAGTTCTGCTAC | JP1BIP | 178 |
| MKW0116 | AACGCCTTTTTCAACTTCTA | JP1LF | 179 |
| MKW0117 | AGATGGCACTTGTGGCTTAG | JP2F3 | 180 |
| MKW0118 | CGAAGAAGAACCTTGCGGTA | JP2B3 | 181 |
| MKW0119 | GCAGTTCGAGCATCCGAACGTTGGCGTTTTGCCTCAACTTG | JP2FIP | 182 |
| MKW0120 | TCGAAGGCATTCAGTACGGTCGACTGGTATTTCGCCCACATG | JP2BIP | 183 |
| MKW0121 | GGTGAGACACTTGGTGTCCTTGTC | JP2LB | 184 |
| MKW0122 | CCTCAACTTGAACAGCCCT | JP5F3 | 185 |
| MKW0123 | ACGAAGAAGAACCTTGCGG | JP5B3 | 186 |
| MKW0124 | GCCTTCGAGTTCTGCTACCAGCTCATCAAACGTTCGGATGCT | JP5FIP | 187 |
| MKW0125 | ACGGTCGTAGTGGTGAGACACTTAAGCCACTGGTATTTCGCC | JP5BIP | 188 |
| MKW0126 | TGACCATGAGGTGCAGTTCG | JP5LF | 189 |
| MKW0127 | TGGTGTCCTTGTCCCTCATGT | JP5LB | 190 |
| MKW0128 | CCTCAACTTGAACAGCCCT | JP6F3 | 191 |
| MKW0129 | TATGGCCACCAGCTCCTT | JP6B3 | 192 |
| MKW0130 | CGACCGTACTGAATGCCTTCGACGTTCGGATGCTCGAACTG | JP6FIP | 193 |
| MKW0131 | GACACTTGGTGTCCTTGTCCCTAGAAGAACCTTGCGGTAAGC | JP6BIP | 194 |
| MKW0132 | CATGTGGGCGAAATACCAGTG | JP6LB | 195 |
| MKW0133 | CTTATCAGAGGCACGTCAA | JP9F3 | 196 |
| MKW0134 | TACGACCGTACTGAATGC | JP9B3 | 197 |
| MKW0135 | TCAAGTTGAGGCAAAACGCCTCTTAAAGATGGCACTTGTGG | JP9FIP | 198 |
| MKW0136 | AGCCCTATGTGTTCATCAAACGTTCTTCGAGTTCTGCTACCA | JP9BIP | 199 |
| MKW0137 | TTTTTCAACTTCTACTAAG | JP9LF | 200 |
| MKW0138 | CATCTTAAAGATGGCACTTGT | JP10F3 | 201 |
| MKW0139 | AGTGTCTCACCACTACGA | JP10B3 | 202 |
| MKW0140 | GTTTGATGAACACATAGGGCTGTTGGCTTAGTAGAAGTTGAAAAAGG | JP10FIP | 203 |
| MKW0141 | GTTCGGATGCTCGAACTGCACCGTACTGAATGCCTTCG | JP10BIP | 204 |
| MKW0142 | CAAGTTGAGGCAAAACG | JP10LF | 205 |
| MKW0143 | GATGGCACTTGTGGCTTA | JP11F3 | 206 |
| MKW0144 | AGGACACCAAGTGTCTCA | JP11B3 | 207 |
| MKW0145 | CCGAACGTTTGATGAACACATAGGGTAGAAGTTGAAAAAGGCGT | JP11FIP | 208 |
| MKW0146 | ATGCTCGAACTGCACCTCATCCACTACGACCGTACTGAA | JP11BIP | 209 |
| MKW0147 | GCTGTTCAAGTTGAGGCAAA | JP11LF | 210 |
| MKW0148 | GTAGAAGTTGAAAAAGGCGTT | JP12F3 | 211 |
| MKW0149 | GAAGAAGAACCTTGCGGT | JP12B3 | 212 |
| MKW0150 | ACCATGAGGTGCAGTTCGAGGCCTCAACTTGAACAGCC | JP12FIP | 213 |
| MKW0151 | AGAACTCGAAGGCATTCAGTACGAAGCCACTGGTATTTCGC | JP12BIP | 214 |
| MKW0152 | TCCGAACGTTTGATGAACACATAG | JP12LF | 215 |
| MKW0153 | GTCGTAGTGGTGAGACACTTGG | JP12LB | 216 |
| MKW0154 | TCAAACGTTCGGATGCTC | JP13F3 | 217 |
| MKW0155 | CCAGCTCCTTTATTACCGT | JP13B3 | 218 |
| MKW0156 | CGTACTGAATGCCTTCGAGTTCTGAACTGCACCTCATGGTC | JP13FIP | 219 |
| MKW0157 | GTCGTAGTGGTGAGACACTTGGACGAAGAAGAACCTTGCG | JP13BIP | 220 |
| MKW0158 | GCTACCAGCTCAACCATAACAT | JP13LF | 221 |
| MKW0159 | CTTGTCCCTCATGTGGGCGAA | JP13LB | 222 |
| XL-600 | TGTTATGGAGTGTCTCCTACT | LnS1-F3 | 223 |
| XL-601 | CCAACCTTAGAATCAAGATTGT | LnS1-B3 | 224 |
| XL-602 | CGATTTGTCTGACTTCATCACCTCTAAATGATCTCTGCTTTACTAATGTC | LnS1-FIP | 225 |
| XL-603 | CTCCAGGGCAAACTGGAAAGCAAGCTATAACGCAGCCT | LnS1-BIP | 226 |
| XL-604 | CAACAGAATCTATTGTTAGATTTCC | LnS2-F3 | 227 |
| XL-605 | AGACATTAGTAAAGCAGAGATCA | LnS2-B3 | 228 |
| XL-606 | TTCTCTTCCTGTTCCAAGCATAAACCTTTTGGTGAAGTTTTTAACG | LnS2-FIP | 229 |
| XL-607 | ACTGTGTTGCTGATTATTCTGTCCAATTTAGTAGGAGACACTCCAT | LnS2-BIP | 230 |
| XL-608 | CCGCATCATTTTCCACTTTTAAGTG | LnS2-LB | 231 |
| XL-609 | CAACAGAATCTATTGTTAGATTTCC | LnS3-F3 | 232 |
| XL-610 | AGACATTAGTAAAGCAGAGATCA | LnS3-B3 | 233 |
| XL-611 | GTTCCAAGCATAAACAGATGCAAATCAAACTTGTGCCCTTTTGG | LnS3-FIP | 234 |
| XL-612 | ACTGTGTTGCTGATTATTCTGTCCAATTTAGTAGGAGACACTCCAT | LnS3-BIP | 235 |
| XL-613 | TGATCTCTGCTTTACTAATGTCT | LnS4-F3 | 236 |
| XL-614 | TGAGATTAGACTTCCTAAACAATC | LnS4-B3 | 237 |
| XL-615 | CAGTTTGCCCTGGAGCGATTATGCAGATTCATTTGTAATTAGAGG | LnS4-FIP | 238 |
| XL-616 | TACCAGATGATTTTACAGGCTGCCCACCAACCTTAGAATCAAGA | LnS4-BIP | 239 |
| XL-617 | ACTTTTAAGTGTTATGGAGTGTC | LnS5-F3 | 240 |
| XL-618 | CCAACCTTAGAATCAAGATTGT | LnS5-B3 | 241 |
| XL-619 | CGATTTGTCTGACTTCATCACCTCTTCCTACTAAATTAAATGATCTCTGC | LnS5-FIP | 242 |
| XL-620 | CTCCAGGGCAAACTGGAAAGTCCAAGCTATAACGCAGC | LnS5-BIP | 243 |
| XL-621 | TGTTATGGAGTGTCTCCTACT | LnS6-F3 | 244 |
| XL-622 | CCACCAACCTTAGAATCAAGA | LnS6-B3 | 245 |
| XL-623 | GAGCGATTTGTCTGACTTCATCATGCTTTACTAATGTCTATGCAGAT | LnS6-FIP | 246 |
| XL-624 | GGCAAACTGGAAAGATTGCTGATGTTAGAATTCCAAGCTATAACG | LnS6-BIP | 247 |
| XL-625 | GAGTTGATTTTTGTGGAAAGG | JpS1-F3 | 248 |
| XL-626 | GTTACAAACCAGTGTGTGC | JpS1-B3 | 249 |
| XL-627 | AGGGACATAAGTCACATGCAAGAAGCTATCATCTTATGTCCTTCCCTC | JpS1-FIP | 250 |
| XL-628 | ACAAGAAAAGAACTTCACAACTGCTTTGAAACAAAGACACCTTCA | JpS1-BIP | 251 |
| XL-629 | ACACCATGAGGTGCTGACT | JpS1-LF | 252 |
| XL-630 | TGCCATTTGTCATGATGGAAAAGC | JpS1-LB | 253 |
| XL-631 | GTGACTTATGTCCCTGCA | JpS2-F3 | 254 |
| XL-632 | ACAATTCCTATTACAACATCACAG | JpS2-B3 | 255 |
| XL-633 | ACGAGGAAAGTGTGCTTTTCCAGAAAAGAACTTCACAACTGC | JpS2-FIP | 256 |
| XL-634 | GTTTCAAATGGCACACACTGGTACACAAATGTGTTGTCTGTAG | JpS2-BIP | 257 |
| XL-635 | CCAATTTAATAGTGCTATTGGCA | JpS3-F3 | 258 |
| XL-636 | GCACTTCAGCCTCAACTT | JpS3-B3 | 259 |
| XL-637 | GCATTTTGGTTGACCACATCTTGAAAATTCAAGACTCACTTTCTTCC | JpS3-FIP | 260 |
| XL-638 | GCTTTAAACACGCTTGTTAAACAACTGTCAAGACGTGAAAGGAT | JpS3-BIP | 261 |
| XL-639 | GTTTTCCAAGTGCACTTGCTGT | JpS3-LF | 262 |
| XL-640 | ACAAGAAAAGAACTTCACAACT | JpS5-F3 | 263 |
| XL-641 | TGACAATTCCTATTACAACATCA | JpS5-B3 | 264 |
| XL-642 | GTGCCATTTGAAACAAAGACACCTTGCTCCTGCCATTTGTCAT | JpS5-FIP | 265 |
| XL-643 | ACACTGGTTTGTAACACAAAGGACAGTTACCAGACACAAATGTG | JpS5-BIP | 266 |
| XL-644 | CACGAGGAAAGTGTGCTTTTCCAT | JpS5-LF | 267 |
| XL-645 | GCTATTGGCAAAATTCAAGAC | JpS6-F3 | 268 |
| XL-646 | GCACTTCAGCCTCAACTT | JpS6-B3 | 269 |
| XL-647 | AGCTTGTGCATTTTGGTTGACCTCACTTTCTTCCACAGCA | JpS6-FIP | 270 |
| XL-648 | AACACGCTTGTTAAACAACTTAGCTTGTCAAGACGTGAAAGGAT | JpS6-BIP | 271 |
| XL-649 | CTTATGTCCTTCCCTCAGTC | JpS7-F3 | 272 |
| XL-650 | TGTAGTAATGATTTGTGGTTCAT | JpS7-B3 | 273 |
| XL-651 | GCAGGAGCAGTTGTGAAGTTCTGTAGTCTTCTTGCATGTGACTT | JpS7-FIP | 274 |
| XL-652 | TGTCATGATGGAAAAGCACACTCCTTTGTGTTACAAACCAGTG | JpS7-BIP | 275 |
| XL-653 | CGTGAAGGTGTCTTTGTTTCAAATG | JpS7-LB | 276 |
| XL-654 | ACACAGAATGTTCTCTATGAGA | JpS8-F3 | 277 |
| XL-655 | CGTGAAAGGATATCATTTAAAACAC | JpS8-B3 | 278 |
| XL-656 | ACTTGCTGTGGAAGAAAGTGAGTATTGCCAACCAATTTAATAGTGC | JpS8-FIP | 279 |
| XL-657 | TTGGAAAACTTCAAGATGTGGTCAGCACCAAAATTGGAGCTAAG | JpS8-BIP | 280 |
| XL-658 | TGCACAAGCTTTAAACACGCTTGTT | JpS8-LB | 281 |
| XL-659 | TCAAGACTCACTTTCTTCCA | JpS9-F3 | 282 |
| XL-660 | ATGTCTGCAAACTTTGAAGT | JpS9-B3 | 283 |
| XL-661 | GCGTGTTTAAAGCTTGTGCATTTCAGCAAGTGCACTTGGAA | JpS9-FIP | 284 |
| XL-662 | TGATATCCTTTCACGTCTTGACAAACTGCCTGTGATCAACCTA | JpS9-BIP | 285 |
| XL-663 | TTGAGGCTGAAGTGCAAATTGA | JpS9-LB | 286 |
| XL-664 | ACCTCATGGTGTAGTCTTCT | JpS10-F3 | 287 |
| XL-665 | CACAAATGTGTTGTCTGTAGT | JpS10-B3 | 288 |
| XL-666 | ATGACAAATGGCAGGAGCAGTGCATGTGACTTATGTCCCT | JpS10-FIP | 299 |
| XL-667 | AAGCACACTTTCCTCGTGAAGGTGTGGTTCATAAAAATTCCTTTG | JpS10-BIP | 300 |
| XL-668 | GTTTCAAATGGCACACACTGGTTTG | JpS10-LB | 301 |
| MKW0167 | TCGTGTTGTTTTAGATTTCATCT | N1-1F3 | 302 |
| MKW0168 | TTGAGTGAGAGCGGTGAA | N1-1B3 | 303 |
| MKW0169 | TCCACCAAACGTAATGCGGGCAAACTAAAATGTCTGATAATGGAC | N1-1FIP | 304 |
| MKW0170 | ACTGGCAGTAACCAGAATGGAGCAGTATTATTGGGTAAACCTTG | N1-1BIP | 305 |
| MKW0171 | GTGCATTTCGCTGATTTTGGG | N1-1LF | 306 |
| MKW0172 | ACGAACAAACTAAAATGTCTGA | N1-2F3 | 307 |
| MKW0173 | TTGAGTGAGAGCGGTGAA | N1-2B3 | 308 |
| MKW0174 | TGAATCTGAGGGTCCACCAAAATGGACCCCAAAATCAGC | N1-2FIP | 309 |
| MKW0175 | ACTGGCAGTAACCAGAATGGAGCAGTATTATTGGGTAAACCTTG | N1-2BIP | 310 |
| MKW0176 | CGTAATGCGGGGTGCATTTC | N1-2LF | 311 |
| MKW0177 | GATTTCATCTAAACGAACAAACT | N1-3F3 | 312 |
| MKW0178 | GGGAATTTAAGGTCTTCCTTG | N1-3B3 | 313 |
| MKW0179 | AGGGTCCACCAAACGTAATGCATGTCTGATAATGGACCCCA | N1-3FIP | 314 |
| MKW0180 | GGCGCGATCAAAACAACGTCCATGTTGAGTGAGAGCGG | N1-3BIP | 315 |
| MKW0181 | AAGGTTTACCCAATAATACTGCGTC | N1-3LB | 316 |
| MKW0182 | ACGAACAAACTAAAATGTCTGA | N1-4F3 | 317 |
| MKW0183 | CGAGGGAATTTAAGGTCTTCC | N1-4B3 | 318 |
| MKW0184 | TTACTGCCAGTTGAATCTGAGGGACCCCAAAATCAGCGAAAT | N1-4FIP | 319 |
| MKW0185 | CGATCAAAACAACGTCGGCCTTGCCATGTTGAGTGAGA | N1-4BIP | 320 |
| MKW0186 | AAACGTAATGCGGGGTGC | N1-4LF | 321 |
| MKW0187 | CAAGGTTTACCCAATAATACTGCGT | N1-4LB | 322 |
| MKW0188 | ACTAAAATGTCTGATAATGGACC | N1-5F3 | 323 |
| MKW0189 | CTCGAGGGAATTTAAGGTCT | N1-5B3 | 324 |
| MKW0190 | GTTACTGCCAGTTGAATCTGAGGCCAAAATCAGCGAAATGCA | N1-5FIP | 325 |
| MKW0191 | CGCGATCAAAACAACGTCGGCCATGTTGAGTGAGAGCG | N1-5BIP | 326 |
| MKW0192 | CACCAAACGTAATGCGGGG | N1-5LF | 327 |
| MKW0193 | CAAGGTTTACCCAATAATACTGCGT | N1-5LB | 328 |
| MKW0194 | ACGAACAAACTAAAATGTCTGA | N1-6F3 | 329 |
| MKW0195 | CCTCGAGGGAATTTAAGGT | N1-6B3 | 330 |
| MKW0196 | TTACTGCCAGTTGAATCTGAGGGATGGACCCCAAAATCAGC | N1-6FIP | 331 |
| MKW0197 | GGCCCCAAGGTTTACCCAATCTTCCTTGCCATGTTGAGT | N1-6BIP | 332 |
| MKW0198 | CGTAATGCGGGGTGCATTTC | N1-6LF | 333 |
| MKW0199 | AATACTGCGTCTTGGTTCACCG | N1-6LB | 334 |
| MKW0200 | CAAACTAAAATGTCTGATAATGGAC | N1-7F3 | 335 |
| MKW0201 | CTCGAGGGAATTTAAGGTCT | N1-7B3 | 336 |
| MKW0202 | GTTACTGCCAGTTGAATCTGAGGCCCAAAATCAGCGAAATGC | N1-7FIP | 337 |
| MKW0203 | GGCCCCAAGGTTTACCCAATTCCTTGCCATGTTGAGTG | N1-7BIP | 338 |
| MKW0204 | ACCAAACGTAATGCGGGGT | N1-7LF | 339 |
| MKW0205 | AATACTGCGTCTTGGTTCACC | N1-7LB | 340 |
| MKW0206 | AACACAAGCTTTCGGCAG | N2-1F3 | 341 |
| MKW0207 | TCTTTGTCATCCAATTTGATGG | N2-1B3 | 342 |
| MKW0208 | CGGCCAATGTTTGTAATCAGTTCCCAGAACAAACCCAAGGAAAT | N2-1FIP | 343 |
| MKW0209 | GTTCTTCGGAATGTCGCGCACCTGTGTAGGTCAACCAC | N2-1BIP | 344 |
| MKW0210 | TGATTAGTTCCTGGTCCCCAAA | N2-1LF | 345 |
| MKW0211 | TTGGCATGGAAGTCACACCT | N2-1LB | 346 |
| MKW0212 | AACACAAGCTTTCGGCAG | N2-2F3 | 347 |
| MKW0213 | TTGGATCTTTGTCATCCAATT | N2-2B3 | 348 |
| MKW0214 | CGGCCAATGTTTGTAATCAGTTCCCAGAACAAACCCAAGGAAAT | N2-2FIP | 349 |
| MKW0215 | GTTCTTCGGAATGTCGCGCATGATGGCACCTGTGTAGG | N2-2BIP | 350 |
| MKW0216 | TGATTAGTTCCTGGTCCCCAAA | N2-2LF | 351 |
| MKW0217 | TTGGCATGGAAGTCACACCT | N2-2LB | 352 |
| MKW0218 | TCCAGAACAAACCCAAGG | N2-3F3 | 353 |
| MKW0219 | ATGACTTGATCTTTGAAATTTGG | N2-3B3 | 354 |
| MKW0220 | GCAATTTGCGGCCAATGTTTGAAATTTTGGGGACCAGGA | N2-3FIP | 355 |
| MKW0221 | TGGCATGGAAGTCACACCTTTCCAATTTGATGGCACCTG | N2-3BIP | 356 |
| MKW0222 | CGGGAACGTGGTTGACCT | N2-3LB | 357 |
| MKW0223 | AACACAAGCTTTCGGCAG | N2-4F3 | 358 |
| MKW0224 | GACTTGATCTTTGAAATTTGGATCT | N2-4B3 | 359 |
| MKW0225 | TGCGGCCAATGTTTGTAATCAGCCAAGGAAATTTTGGGGAC | N2-4FIP | 360 |
| MKW0226 | TGGCATGGAAGTCACACCTTATCCAATTTGATGGCACCT | N2-4BIP | 361 |
| MKW0227 | CGGGAACGTGGTTGACCT | N2-4LB | 362 |
| MKW0228 | AACACAAGCTTTCGGCAG | N2-5F3 | 363 |
| MKW0229 | TCTTTGTCATCCAATTTGATGG | N2-5B3 | 364 |
| MKW0230 | TGCGGCCAATGTTTGTAATCAGCAGAACAAACCCAAGGAAAT | N2-5FIP | 365 |
| MKW0231 | GTTCTTCGGAATGTCGCGCACACCTGTGTAGGTCAACC | N2-5BIP | 366 |
| MKW0232 | TGATTAGTTCCTGGTCCCCAAA | N2-5LF | 367 |
| MKW0233 | TTGGCATGGAAGTCACACCT | N2-5LB | 368 |
| MKW0234 | AACACAAGCTTTCGGCAG | N2-6F3 | 369 |
| MKW0235 | GATCTTTGTCATCCAATTTGATG | N2-6B3 | 370 |
| MKW0236 | CGGCCAATGTTTGTAATCAGTTCCAGAACAAACCCAAGGAAAT | N2-6FIP | 371 |
| MKW0237 | GTTCTTCGGAATGTCGCGCACCTGTGTAGGTCAACCAC | N2-6BIP | 372 |
| MKW0238 | TGATTAGTTCCTGGTCCCCAAA | N2-6LF | 373 |
| MKW0239 | TTGGCATGGAAGTCACACCT | N2-6LB | 374 |
| MKW0240 | TAACACAAGCTTTCGGCA | N2-7F3 | 375 |
| MKW0241 | GAAATTTGGATCTTTGTCATCC | N2-7B3 | 376 |
| MKW0242 | GCAATTTGCGGCCAATGTTTGCCAAGGAAATTTTGGGGAC | N2-7FIP | 377 |
| MKW0243 | GTTCTTCGGAATGTCGCGCATTTGATGGCACCTGTGTAG | N2-7BIP | 378 |
| MKW0244 | CAGTTCCTTGTCTGATTAGTTCCTG | N2-7LF | 379 |
| MKW0245 | TTGGCATGGAAGTCACACCT | N2-7LB | 380 |
| MKW0246 | AACACAAGCTTTCGGCAG | N2-8F3 | 381 |
| MKW0247 | CTTTGAAATTTGGATCTTTGTCA | N2-8B3 | 382 |
| MKW0248 | TGCGGCCAATGTTTGTAATCAGCCAAGGAAATTTTGGGGAC | N2-8FIP | 383 |
| MKW0249 | TGGCATGGAAGTCACACCTTTCCAATTTGATGGCACCTG | N2-8BIP | 384 |
| MKW0250 | CGGGAACGTGGTTGACCT | N2-8LB | 385 |
| MKW0251 | AACACAAGCTTTCGGCAG | N2-9F3 | 386 |
| MKW0252 | AGCAAAATGACTTGATCTTTGA | N2-9B3 | 387 |
| MKW0253 | GCAATTTGCGGCCAATGTTTAAGGAAATTTTGGGGACCAG | N2-9FIP | 388 |
| MKW0254 | ATGGAAGTCACACCTTCGGGTCTTTGTCATCCAATTTGATGG | N2-9BIP | 389 |
| MKW0255 | AACGTGGTTGACCTACACAGG | N2-9LB | 390 |
| MKW0256 | GAAATCTGCTGCTGAGGC | N2-10F3 | 391 |
| MKW0257 | AAGGTGTGACTTCCATGC | N2-10B3 | 392 |
| MKW0258 | GGTTTGTTCTGGACCACGTCTAAACGTACTGCCACTAAAGC | N2-10FIP | 393 |
| MKW0259 | ACTGATTACAAACATTGGCCGCGACATTCCGAAGAACGCT | N2-10BIP | 394 |
| MKW0260 | GCCGAAAGCTTGTGTTACATTGTAT | N2-10LF | 395 |
| MKW0261 | TCTTCTCGTTCCTCATCAC | CN-1F3 | 396 |
| MKW0262 | CTTAGAAGCCTCAGCAGC | CN-1B3 | 397 |
| MKW0263 | TCTAGCAGGAGAAGTTCCCCTAGTAGTCGCAACAGTTCAAGAA | CN-1FIP | 398 |
| MKW0264 | CTGCTTGACAGATTGAACCAGCGTGACAGTTTGGCCTTGTT | CN-1BIP | 399 |
| MKW0265 | AAAATGTCTGGTAAAGGCCAACAAC | CN-1LB | 400 |
| MKW0266 | AGTAGGGGAACTTCTCCTG | CN-2F3 | 401 |
| MKW0267 | CTGCCGAAAGCTTGTGTT | CN-2B3 | 402 |
| MKW0268 | GCTGGTTCAATCTGTCAAGCAGTAGAATGGCTGGCAATGG | CN-2FIP | 403 |
| MKW0269 | GCCAACAACAACAAGGCCAAAGTACGTTTTTGCCGAGG | CN-2BIP | 404 |
| MKW0270 | GCAAGAGCAGCATCACCG | CN-2LF | 405 |
| MKW0271 | AGTAGGGGAACTTCTCCTG | CN-3F3 | 406 |
| MKW0272 | CTGCCGAAAGCTTGTGTT | CN-3B3 | 407 |
| MKW0273 | GCTGGTTCAATCTGTCAAGCAGTAGAATGGCTGGCAATGG | CN-3FIP | 408 |
| MKW0274 | AACAAGGCCAAACTGTCACTAACAGTACGTTTTTGCCGAG | CN-3BIP | 409 |
| MKW0275 | GCAAGAGCAGCATCACCG | CN-3LF | 410 |
| MKW0276 | TCCTCATCACGTAGTCGC | CN-4F3 | 411 |
| MKW0277 | GGCTTCTTAGAAGCCTCAG | CN-4B3 | 412 |
| MKW0278 | CCAGCCATTCTAGCAGGAGAAAACAGTTCAAGAAATTCAACTCC | CN-4FIP | 413 |
| MKW0279 | TTGACAGATTGAACCAGCTTGAGACAGCAGATTTCTTAGTGACAGT | CN-4BIP | 414 |
| MKW0280 | GTTCCCCTACTGCTGCCT | CN-4LF | 415 |
| MKW0281 | GCAAAATGTCTGGTAAAGGCCAACA | CN-4LB | 416 |
| MKW0282 | GCTTCTACGCAGAAGGGA | CN-5F3 | 417 |
| MKW0283 | GTGACAGTTTGGCCTTGT | CN-5B3 | 418 |
| MKW0284 | CTACTGCTGCCTGGAGTTGAATTCCTCTTCTCGTTCCTCATC | CN-5FIP | 419 |
| MKW0285 | GCTTTGCTGCTGCTTGACAGTGTTGTTGGCCTTTACCA | CN-5BIP | 420 |
| MKW0286 | CTTGAACTGTTGCGACTACGT | CN-5LF | 421 |
| MKW0287 | ATTGAACCAGCTTGAGAGCAAA | CN-5LB | 422 |
| MKW0288 | GCTGCAATCGTGCTACAACT | CN-8F3 | 423 |
| MKW0289 | TTTGCTCTCAAGCTGGTTCA | CN-8B3 | 424 |
| MKW0290 | TGCGACTACGTGATGAGGAACGTTGCCAAAAGGCTTCTACGC | CN-8FIP | 425 |
| MKW0291 | TCTCCTGCTAGAATGGCTGGCATCTGTCAAGCAGCAGCAAAG | CN-8BIP | 426 |
| MKW0292 | TTGACTGCCGCCTCTGC | CN-8LF | 427 |
| gaaatTAATACGACTCACTATAGGGTGATTAGTTCCTGGTCCCCAAA | N2-2LFT7 | 528 | |
| gaaatTAATACGACTCACTATAGGGACACCATGAGGTGCTGACT | Jps1LFT7 | 529 |
測試引子組且將每組分級為4：無擴增/極差擴增；3：較差靈敏度及緩慢擴增或2/2 NTC陽性；2：良好靈敏度及緩慢擴增或較差靈敏度及快速擴增；或1：良好靈敏度及速度。良好速度：平均3000 ＜18 : 平均30 ＜25；良好靈敏度：對於30 cp為2/2。LAMP引子篩選之結果展示於表21及表22中。
表21
表22
| LAMP 引子 | |||||||
| 擴增子 ID | F3 | B3 | FIP | BIP | LF | LB | 引子組 ID |
| B1 | XL-500 | XL-501 | XL-502 | XL-503 | XL-504 | B1 | |
| B2 | XL-505 | XL-506 | XL-507 | XL-508 | XL-509 | B2 | |
| B3 | XL-510 | XL-511 | XL-512 | XL-513 | XL-514 | XL-515 | B3 |
| B4 | XL-516 | XL-517 | XL-518 | XL-519 | XL-520 | B4 | |
| B5 | XL-521 | XL-522 | XL-523 | XL-524 | XL-525 | XL-526 | B5 |
| B6 | XL-527 | XL-528 | XL-529 | XL-530 | XL-531 | B6 | |
| B7 | XL-532 | XL-533 | XL-534 | XL-535 | XL-536 | B7 | |
| B8 | XL-537 | XL-538 | XL-539 | XL-540 | XL-541 | B8 | |
| HK1 | XL-542 | XL-543 | XL-544 | XL-545 | HK1 | ||
| HK2 | XL-546 | XL-547 | XL-548 | XL-549 | XL-550 | HK2 | |
| HK3 | XL-551 | XL-552 | XL-553 | XL-554 | XL-555 | HK3 | |
| HK4 | XL-556 | XL-557 | XL-558 | XL-559 | XL-560 | HK4 | |
| HK5 | XL-561 | XL-562 | XL-563 | XL-564 | XL-565 | XL-566 | HK5 |
| HK6 | XL-567 | XL-568 | XL-569 | XL-570 | XL-571 | HK6 | |
| HK7 | XL-572 | XL-573 | XL-574 | XL-575 | XL-576 | XL-577 | HK7 |
| HK8 | XL-578 | XL-579 | XL-580 | XL-581 | XL-582 | HK8 | |
| JP1 | MKW0112 | MKW0113 | MKW0114 | MKW0115 | MKW0116 | JP1 | |
| JP2 | MKW0117 | MKW0118 | MKW0119 | MKW0120 | MKW0121 | JP2 | |
| JP5 | MKW0122 | MKW0123 | MKW0124 | MKW0125 | MKW0126 | MKW0127 | JP5 |
| JP6 | MKW0128 | MKW0129 | MKW0130 | MKW0131 | MKW0132 | JP6 | |
| JP9 | MKW0133 | MKW0134 | MKW0135 | MKW0136 | MKW0137 | JP9 | |
| JP10 | MKW0138 | MKW0139 | MKW0140 | MKW0141 | MKW0142 | JP10 | |
| JP11 | MKW0143 | MKW0144 | MKW0145 | MKW0146 | MKW0147 | JP11 | |
| JP12 | MKW0148 | MKW0149 | MKW0150 | MKW0151 | MKW0152 | MKW0153 | JP12 |
| JP13 | MKW0154 | MKW0155 | MKW0156 | MKW0157 | MKW0158 | MKW0159 | JP13 |
| LnS1 | XL-600 | XL-601 | XL-602 | XL-603 | LnS1 | ||
| LnS2 | XL-604 | XL-605 | XL-606 | XL-607 | XL-608 | LnS2 | |
| LnS3 | XL-609 | XL-610 | XL-611 | XL-612 | LnS3 | ||
| LnS4 | XL-613 | XL-614 | XL-615 | XL-616 | LnS4 | ||
| LnS5 | XL-617 | XL-618 | XL-619 | XL-620 | LnS5 | ||
| LnS6 | XL-621 | XL-622 | XL-623 | XL-624 | LnS6 | ||
| JpS1 | XL-625 | XL-626 | XL-627 | XL-628 | XL-629 | XL-630 | JpS1 |
| JpS2 | XL-631 | XL-632 | XL-633 | XL-634 | JpS2 | ||
| JpS3 | XL-635 | XL-636 | XL-637 | XL-638 | XL-639 | JpS3 | |
| JpS5 | XL-640 | XL-641 | XL-642 | XL-643 | XL-644 | JpS5 | |
| JpS6 | XL-645 | XL-646 | XL-647 | XL-648 | JpS6 | ||
| JpS7 | XL-649 | XL-650 | XL-651 | XL-652 | XL-653 | JpS7 | |
| JpS8 | XL-654 | XL-655 | XL-656 | XL-657 | XL-658 | JpS8 | |
| JpS9 | XL-659 | XL-660 | XL-661 | XL-662 | XL-663 | JpS9 | |
| JpS10 | XL-664 | XL-665 | XL-666 | XL-667 | XL-668 | JpS10 | |
| N1-1 | MKW0167 | MKW0168 | MKW0169 | MKW0170 | MKW0171 | N1-1 | |
| N1-2 | MKW0172 | MKW0173 | MKW0174 | MKW0175 | MKW0176 | N1-2 | |
| N1-3 | MKW0177 | MKW0178 | MKW0179 | MKW0180 | MKW0181 | N1-3 | |
| N1-4 | MKW0182 | MKW0183 | MKW0184 | MKW0185 | MKW0186 | MKW0187 | N1-4 |
| N1-5 | MKW0188 | MKW0189 | MKW0190 | MKW0191 | MKW0192 | MKW0193 | N1-5 |
| N1-6 | MKW0194 | MKW0195 | MKW0196 | MKW0197 | MKW0198 | MKW0199 | N1-6 |
| N1-7 | MKW0200 | MKW0201 | MKW0202 | MKW0203 | MKW0204 | MKW0205 | N1-7 |
| N2-1 | MKW0206 | MKW0207 | MKW0208 | MKW0209 | MKW0210 | MKW0211 | N2-1 |
| N2-2 | MKW0212 | MKW0213 | MKW0214 | MKW0215 | MKW0216 | MKW0217 | N2-2 |
| N2-3 | MKW0218 | MKW0219 | MKW0220 | MKW0221 | MKW0222 | N2-3 | |
| N2-4 | MKW0223 | MKW0224 | MKW0225 | MKW0226 | MKW0227 | N2-4 | |
| N2-5 | MKW0228 | MKW0229 | MKW0230 | MKW0231 | MKW0232 | MKW0233 | N2-5 |
| N2-6 | MKW0234 | MKW0235 | MKW0236 | MKW0237 | MKW0238 | MKW0239 | N2-6 |
| N2-7 | MKW0240 | MKW0241 | MKW0242 | MKW0243 | MKW0244 | MKW0245 | N2-7 |
| N2-8 | MKW0246 | MKW0247 | MKW0248 | MKW0249 | MKW0250 | N2-8 | |
| N2-9 | MKW0251 | MKW0252 | MKW0253 | MKW0254 | MKW0255 | N2-9 | |
| N2-10 | MKW0256 | MKW0257 | MKW0258 | MKW0259 | MKW0260 | N2-10 | |
| CN-1 | MKW0261 | MKW0262 | MKW0263 | MKW0264 | MKW0265 | CN-1 | |
| CN-2 | MKW0266 | MKW0267 | MKW0268 | MKW0269 | MKW0270 | CN-2 | |
| CN-3 | MKW0271 | MKW0272 | MKW0273 | MKW0274 | MKW0275 | CN-3 | |
| CN-4 | MKW0276 | MKW0277 | MKW0278 | MKW0279 | MKW0280 | MKW0281 | CN-4 |
| CN-5 | MKW0282 | MKW0283 | MKW0284 | MKW0285 | MKW0286 | MKW0287 | CN-5 |
| CN-8 | MKW0288 | MKW0289 | MKW0290 | MKW0291 | MKW0292 | CN-8 |
| Ct 值 | |||||||||
| 引子組 ID | ~3000 cp | ~30 cp | NTC | 平均3000 cp | 平均30 cp | 等級 | |||
| B1 | 21.5 | n/d | n/d | 21.6 | 12.0 | 30.2 | 21.5 | 21.6 | 4 |
| B2 | 17.3 | 17.3 | 23.9 | 29.2 | n/d | n/d | 17.3 | 26.6 | 2 |
| B3 | 12.2 | 11.7 | 30.8 | n/d | n/d | n/d | 12.0 | 30.8 | 2 |
| B4 | 19.3 | 20.6 | 33.0 | 22.9 | n/d | n/d | 19.9 | 28.0 | 3 |
| B5 | 18.0 | 16.4 | 29.4 | n/d | n/d | n/d | 17.2 | 29.4 | 2 |
| B6 | 24.9 | 26.2 | n/d | n/d | n/d | n/d | 25.6 | #DIV/0! | 3 |
| B7 | 21.4 | 23.2 | n/d | 37.2 | n/d | n/d | 22.3 | 37.2 | 3 |
| B8 | 18.9 | 19.1 | n/d | n/d | 34.1 | 35.5 | 19.0 | #DIV/0! | 3 |
| HK1 | 37.4 | n/d | n/d | n/d | n/d | n/d | 37.4 | #DIV/0! | 4 |
| HK2 | 26.1 | 34.2 | n/d | n/d | n/d | n/d | 30.1 | #DIV/0! | 3 |
| HK3 | 17.1 | 17.1 | 24.2 | 25.8 | n/d | n/d | 17.1 | 25.0 | 1 |
| HK4 | 19.2 | 18.8 | n/d | n/d | n/d | n/d | 19.0 | #DIV/0! | 2 |
| HK5 | 11.9 | 12.0 | 16.8 | 17.0 | n/d | n/d | 11.9 | 16.9 | 1 |
| HK6 | 20.1 | 19.5 | n/d | n/d | n/d | n/d | 19.8 | #DIV/0! | 2 |
| HK7 | 10.8 | 11.0 | n/d | n/d | n/d | n/d | 10.9 | #DIV/0! | 2 |
| HK8 | 24.4 | 21.5 | 34.6 | n/d | n/d | n/d | 22.9 | 34.6 | 3 |
| JP1 | 20.9 | 21.7 | 30.6 | 26.8 | n/d | n/d | 21.3 | 28.7 | 2 |
| JP2 | 18.4 | 18.4 | 29.1 | 29.3 | n/d | n/d | 18.4 | 29.2 | 2 |
| JP5 | 9.7 | 9.4 | 13.7 | 12.6 | 47.6 | n/d | 9.6 | 13.1 | 1 |
| JP6 | 17.8 | 19.4 | 27.6 | 30.1 | n/d | 39.9 | 18.6 | 28.8 | 2 |
| JP9 | 37.2 | 39.2 | 51.7 | n/d | n/d | n/d | 38.2 | 51.7 | 3 |
| JP10 | 48.8 | 45.9 | n/d | n/d | n/d | n/d | 47.4 | #DIV/0! | 3 |
| JP11 | 31.4 | 30.3 | n/d | 49.9 | n/d | n/d | 30.8 | 49.9 | 3 |
| JP12 | 16.1 | 16.8 | n/d | 21.9 | n/d | n/d | 16.5 | 21.9 | 2 |
| JP13 | 13.6 | 14.4 | 22.9 | 18.6 | n/d | n/d | 14.0 | 20.7 | 2 |
| LnS1 | n/d | n/d | 53.3 | 38.7 | n/d | n/d | #DIV/0! | 46.0 | 4 |
| LnS2 | 27.7 | 26.9 | 33.2 | 44.7 | n/d | n/d | 27.3 | 39.0 | 3 |
| LnS3 | 38.6 | 35.6 | n/d | n/d | n/d | n/d | 37.1 | #DIV/0! | 3 |
| LnS4 | 26.4 | 26.6 | 36.5 | 34.0 | n/d | n/d | 26.5 | 35.2 | 3 |
| LnS5 | n/d | n/d | n/d | n/d | n/d | n/d | #DIV/0! | #DIV/0! | 4 |
| LnS6 | n/d | n/d | n/d | n/d | n/d | 51.1 | #DIV/0! | #DIV/0! | 4 |
| JpS1 | 11.8 | 12.0 | 15.8 | 24.0 | n/d | n/d | 11.9 | 19.9 | 1 |
| JpS2 | 29.2 | 28.5 | 57.8 | 56.3 | n/d | n/d | 28.9 | 57.0 | 3 |
| JpS3 | 21.9 | 22.3 | n/d | 26.3 | n/d | n/d | 22.1 | 26.3 | 3 |
| JpS5 | 17.9 | 18.0 | 26.0 | 21.7 | n/d | n/d | 17.9 | 23.9 | 1 |
| JpS6 | 33.3 | 33.2 | 38.0 | 52.7 | 44.9 | 45.3 | 33.2 | 45.4 | 3 |
| JpS7 | 30.4 | 30.4 | 39.8 | 38.7 | n/d | n/d | 30.4 | 39.3 | 3 |
| JpS8 | 15.7 | 16.5 | 20.9 | 19.3 | n/d | n/d | 16.1 | 20.1 | 1 |
| JpS9 | 20.1 | 19.3 | 26.5 | 26.8 | n/d | n/d | 19.7 | 26.7 | 2 |
| JpS10 | 19.9 | 19.8 | n/d | 27.4 | n/d | n/d | 19.9 | 27.4 | 2 |
| N1-1 | 21.946 | 25.496 | n/d | n/d | n/d | n/d | 23.7 | #DIV/0! | 3 |
| N1-2 | 27.221 | 24.863 | 34.189 | n/d | 31.641 | n/d | 26.0 | 34.2 | 3 |
| N1-3 | 24.853 | 25.655 | n/d | 27.966 | n/d | n/d | 25.3 | 28.0 | 3 |
| N1-4 | 15.157 | 14.626 | 34.418 | n/d | n/d | n/d | 14.9 | 34.4 | 2 |
| N1-5 | 12.444 | 11.89 | n/d | 24.671 | n/d | n/d | 12.2 | 24.7 | 2 |
| N1-6 | 11.749 | 11.83 | 18.703 | 19.939 | n/d | n/d | 11.8 | 19.3 | 1 |
| N1-7 | 12.871 | 13.762 | 21.513 | 29.409 | n/d | n/d | 13.3 | 25.5 | 2 |
| N2-1 | 16.2 | 15.2 | 23.9 | 20.0 | n/d | n/d | 15.7 | 21.9 | 1 |
| N2-2 | 16.5 | 16.6 | 23.0 | 22.5 | n/d | n/d | 16.5 | 22.7 | 1 |
| N2-3 | 19.1 | 18.3 | 25.5 | 23.3 | n/d | n/d | 18.7 | 24.4 | 2 |
| N2-4 | 16.8 | 17.7 | 24.5 | 25.2 | n/d | 29.4 | 17.2 | 24.8 | 1 |
| N2-5 | 15.4 | 14.5 | 20.9 | 18.9 | 34.5 | n/d | 15.0 | 19.9 | 1 |
| N2-6 | 15.9 | 16.1 | 22.8 | 21.2 | n/d | n/d | 16.0 | 22.0 | 1 |
| N2-7 | 11.1 | 10.5 | 16.2 | 14.3 | n/d | n/d | 10.8 | 15.3 | 1 |
| N2-8 | 15.3 | 15.1 | 19.1 | 17.9 | n/d | n/d | 15.2 | 18.5 | 1 |
| N2-9 | 20.1 | 21.9 | 37.7 | 27.9 | n/d | n/d | 21.0 | 32.8 | 2 |
| N2-10 | 27.5 | 26.6 | 38.4 | 32.2 | n/d | 38.3 | 27.1 | 35.3 | 2 |
| CN-1 | 15.9 | 16.4 | 21.8 | 19.1 | 30.1 | 25.0 | 16.1 | 20.4 | 3 |
| CN-2 | n/d | 39.8 | n/d | n/d | n/d | n/d | 39.8 | #DIV/0! | 4 |
| CN-3 | n/d | n/d | n/d | 34.1 | n/d | n/d | #DIV/0! | 34.1 | 4 |
| CN-4 | 12.9 | 13.3 | 18.4 | 17.7 | n/d | n/d | 13.1 | 18.1 | 1 |
| CN-5 | 12.5 | 13.4 | 16.2 | 15.7 | n/d | n/d | 13.0 | 16.0 | 1 |
| CN-8 | 18.5 | 17.8 | 26.2 | 26.7 | n/d | n/d | 18.1 | 26.5 | 2 |
應注意，一些算法設計之引子完全無法擴增目標。因此，需要該等大規模測試分析以憑經驗鑑別可用於擴增SARS-CoV-2核酸之LAMP引子組。
已鑑別出可行的引子組，需要構築包含crRNA之嚮導RNA，其結合至藉由LAMP引子組擴增之核酸。設計2至5種嚮導物用於給定可行的LAMP引子組。為設計嚮導物，選擇位於F2結合區與F1c結合區之間、F1c結合區與B1c結合區之間或B1c結合區與B2結合區之間的28 nt區域進行嚮導物篩選。嚮導物係藉由可用算法來設計以與組中之任一LAMP引子具有＜10 bp重疊。在初始引子篩選後，使用Sherlock反應測試潛在嚮導物。藉由比較自目標LAMP擴增觀察到之信號對自無目標LAMP擴增觀察到之信號，所選嚮導物顯示高信號對雜訊比。經設計嚮導物列於表23中
表23
| 名稱 | 序列 | 設計，未測試 | SEQ ID NO |
| XLcr-600 | gatttagactaccccaaaaacgaaggggactaaaacTCTGGTAATTTATAATTATAATCAGCAA | X | 428 |
| XLcr-601 | gatttagactaccccaaaaacgaaggggactaaaacAAAATCATCTGGTAATTTATAATTATAA | X | 429 |
| XLcr-602 | gatttagactaccccaaaaacgaaggggactaaaacCTGGTAATTTATAATTATAATCAGCAAT | X | 430 |
| XLcr-603 | gatttagactaccccaaaaacgaaggggactaaaacACACTTAAAAGTGGAAAATGATGCGGAA | X | 431 |
| XLcr-604 | gatttagactaccccaaaaacgaaggggactaaaacAAAAGTGGAAAATGATGCGGAATTATAT | X | 432 |
| XLcr-605 | gatttagactaccccaaaaacgaaggggactaaaacACACTTAAAAGTGGAAAATGATGCGGAA | X | 433 |
| XLcr-606 | gatttagactaccccaaaaacgaaggggactaaaacTAAAAGTGGAAAATGATGCGGAATTATA | X | 434 |
| XLcr-607 | gatttagactaccccaaaaacgaaggggactaaaacGAAAGATTGCTGATTATAATTATAAATT | X | 435 |
| XLcr-608 | gatttagactaccccaaaaacgaaggggactaaaacTGCGTTATAGCTTGGAATTCTAACAATC | X | 436 |
| XLcr-609 | gatttagactaccccaaaaacgaaggggactaaaacATTACAAATGAATCTGCATAGACATTAG | X | 437 |
| XLcr-610 | gatttagactaccccaaaaacgaaggggactaaaacTCTGGTAATTTATAATTATAATCAGCAA | X | 438 |
| XLcr-611 | gatttagactaccccaaaaacgaaggggactaaaacCCTGTAAAATCATCTGGTAATTTATAAT | X | 439 |
| XLcr-612 | gatttagactaccccaaaaacgaaggggactaaaacGCCTGTAAAATCATCTGGTAATTTATAA | X | 440 |
| XLcr-613 | gatttagactaccccaaaaacgaaggggactaaaacAAAGTGTGCTTTTCCATCATGACAAATG | X | 441 |
| XLcr-614 | gatttagactaccccaaaaacgaaggggactaaaacGTGTGCTTTTCCATCATGACAAATGGCA | X | 442 |
| XLcr-615 | gatttagactaccccaaaaacgaaggggactaaaacTGGTTCATAAAAATTCCTTTGTGTTACA | X | 443 |
| XLcr-616 | gatttagactaccccaaaaacgaaggggactaaaacATTTGTGGTTCATAAAAATTCCTTTGTG | X | 444 |
| XLcr-617 | gatttagactaccccaaaaacgaaggggactaaaacCATTTAAAACACTTGAAATTGCACCAAA | X | 445 |
| XLcr-618 | gatttagactaccccaaaaacgaaggggactaaaacAAACACTTGAAATTGCACCAAAATTGGA | X | 446 |
| XLcr-619 | gatttagactaccccaaaaacgaaggggactaaaacTCTGTAGTAATGATTTGTGGTTCATAAA | X | 447 |
| XLcr-620 | gatttagactaccccaaaaacgaaggggactaaaacTGATGGAAAAGCACACTTTCCTCGTGAA | X | 448 |
| XLcr-621 | gatttagactaccccaaaaacgaaggggactaaaacAAGTGCACTTGGAAAACTTCAAGATGTG | X | 449 |
| XLcr-622 | gatttagactaccccaaaaacgaaggggactaaaacCATTTAAAACACTTGAAATTGCACCAAA | X | 450 |
| XLcr-623 | gatttagactaccccaaaaacgaaggggactaaaacCCATTTGAAACAAAGACACCTTCACGAG | X | 451 |
| XLcr-624 | gatttagactaccccaaaaacgaaggggactaaaacGTGCCATTTGAAACAAAGACACCTTCAC | X | 452 |
| XLcr-625 | gatttagactaccccaaaaacgaaggggactaaaacACAAGCGTGTTTAAAGCTTGTGCATTTT | X | 453 |
| XLcr-626 | gatttagactaccccaaaaacgaaggggactaaaacGCACCAAAATTGGAGCTAAGTTGTTTAA | X | 454 |
| XLcr-627 | gatttagactaccccaaaaacgaaggggactaaaacTGAAATTGCACCAAAATTGGAGCTAAGT | X | 455 |
| XLcr-628 | gatttagactaccccaaaaacgaaggggactaaaacCCAGTGTGTGCCATTTGAAACAAAGACA | X | 456 |
| XLcr-629 | gatttagactaccccaaaaacgaaggggactaaaacACAAACCAGTGTGTGCCATTTGAAACAA | X | 457 |
| MKW0293 | gatttagactaccccaaaaacgaaggggactaaaacCCGACGTTGTTTTGATCGCGCCCCACTG | X | 458 |
| MKW0294 | gatttagactaccccaaaaacgaaggggactaaaacGTGGGGCGCGATCAAAACAACGTCGGCC | X | 459 |
| MKW0295 | gatttagactaccccaaaaacgaaggggactaaaacGAACGCAGTGGGGCGCGATCAAAACAAC | X | 460 |
| MKW0296 | gatttagactaccccaaaaacgaaggggactaaaacGGCAGTAACCAGAATGGAGAACGCAGTG | X | 461 |
| MKW0297 | gatttagactaccccaaaaacgaaggggactaaaacCGCCCCACTGCGTTCTCCATTCTGGTTA | 462 | |
| MKW0298 | gatttagactaccccaaaaacgaaggggactaaaacGCTGAAGCGCTGGGGGCAAATTGTGCAA | 463 | |
| MKW0299 | gatttagactaccccaaaaacgaaggggactaaaacGAAGCGCTGGGGGCAAATTGTGCAATTT | 464 | |
| MKW0300 | gatttagactaccccaaaaacgaaggggactaaaacCAATTTGCCCCCAGCGCTTCAGCGTTCT | 465 | |
| MKW0301 | gatttagactaccccaaaaacgaaggggactaaaacCCCCCAGCGCTTCAGCGTTCTTCGGAAT | 466 | |
| MKW0302 | gatttagactaccccaaaaacgaaggggactaaaacAAATTGCACAATTTGCCCCCAGCGCTTC | 467 | |
| MKW0303 | gatttagactaccccaaaaacgaaggggactaaaacAATGGCGGTGATGCTGCTCTTGCTTTGC | 468 | |
| MKW0304 | gatttagactaccccaaaaacgaaggggactaaaacGCAAAGCAAGAGCAGCATCACCGCCATT | 469 | |
| MKW0305 | gatttagactaccccaaaaacgaaggggactaaaacTGAGAGCAAAATGTCTGGTAAAGGCCAA | X | 470 |
| MKW0306 | gatttagactaccccaaaaacgaaggggactaaaacGGTGATGCTGCTCTTGCTTTGCTGCTGC | 471 | |
| MKW0307 | gatttagactaccccaaaaacgaaggggactaaaacGCAGCAGCAAAGCAAGAGCAGCATCACC | 472 | |
| XLcr-500 | gatttagactaccccaaaaacgaaggggactaaaacTGACAAATGTTAAAAACACTATTAGCAT | X | 473 |
| XLcr-501 | gatttagactaccccaaaaacgaaggggactaaaacAATGTTAAAAACACTATTAGCATAAGCA | X | 474 |
| XLcr-502 | gatttagactaccccaaaaacgaaggggactaaaacCAAATGTTAAAAACACTATTAGCATAAG | X | 475 |
| XLcr-503 | gatttagactaccccaaaaacgaaggggactaaaacTGACAGCTTGACAAATGTTAAAAACACT | X | 476 |
| XLcr-504 | gatttagactaccccaaaaacgaaggggactaaaacCGTGACAGCTTGACAAATGTTAAAAACA | X | 477 |
| XLcr-505 | gatttagactaccccaaaaacgaaggggactaaaacTGTTGTAGCTTGTCACACCGTTTCTATA | 478 | |
| XLcr-506 | gatttagactaccccaaaaacgaaggggactaaaacCATCTCCTGATGAGGTTCCACCTGGTTT | 479 | |
| XLcr-507 | gatttagactaccccaaaaacgaaggggactaaaacCTCCTGATGAGGTTCCACCTGGTTTAAC | 480 | |
| XLcr-508 | gatttagactaccccaaaaacgaaggggactaaaacTAGCATAAGCAGTTGTGGCATCTCCTGA | X | 481 |
| XLcr-509 | gatttagactaccccaaaaacgaaggggactaaaacTAGAAACGGTGTGACAAGCTACAACACG | 482 | |
| XLcr-510 | gatttagactaccccaaaaacgaaggggactaaaacACGGTGTGACAAGCTACAACACGTTGTA | 483 | |
| XLcr-511 | gatttagactaccccaaaaacgaaggggactaaaacCATCTCCTGATGAGGTTCCACCTGGTTT | 484 | |
| XLcr-512 | gatttagactaccccaaaaacgaaggggactaaaacCCTGATGAGGTTCCACCTGGTTTAACAT | 485 | |
| XLcr-513 | gatttagactaccccaaaaacgaaggggactaaaacCCTGATGAGGTTCCACCTGGTTTAACAT | X | 486 |
| XLcr-514 | gatttagactaccccaaaaacgaaggggactaaaacTGAGGTTCCACCTGGTTTAACATATAGT | X | 487 |
| XLcr-515 | gatttagactaccccaaaaacgaaggggactaaaacTGACAAATGTTAAAAACACTATTAGCAT | X | 488 |
| XLcr-516 | gatttagactaccccaaaaacgaaggggactaaaacAATGTTAAAAACACTATTAGCATAAGCA | X | 489 |
| XLcr-517 | gatttagactaccccaaaaacgaaggggactaaaacTGACAGCTTGACAAATGTTAAAAACACT | X | 490 |
| XLcr-518 | gatttagactaccccaaaaacgaaggggactaaaacCAGCTTGACAAATGTTAAAAACACTATT | X | 491 |
| XLcr-519 | gatttagactaccccaaaaacgaaggggactaaaacAATCAAATCCAATAGAATGATGCCAACA | X | 492 |
| XLcr-520 | gatttagactaccccaaaaacgaaggggactaaaacATCAAATCCAATAGAATGATGCCAACAG | X | 493 |
| XLcr-521 | gatttagactaccccaaaaacgaaggggactaaaacTAATCAAATCCAATAGAATGATGCCAAC | X | 494 |
| XLcr-522 | gatttagactaccccaaaaacgaaggggactaaaacACACGCTTAACAAAGCACTCGTGGACAG | X | 495 |
| XLcr-523 | gatttagactaccccaaaaacgaaggggactaaaacCGCTTAACAAAGCACTCGTGGACAGCTA | X | 496 |
| XLcr-524 | gatttagactaccccaaaaacgaaggggactaaaacCCGCATTAATCTTCAGTTCATCACCAAT | X | 497 |
| XLcr-525 | gatttagactaccccaaaaacgaaggggactaaaacACAAGCCGCATTAATCTTCAGTTCATCA | X | 498 |
| XLcr-526 | gatttagactaccccaaaaacgaaggggactaaaacACACCTAGTCATGATTGCATCACAACTA | X | 499 |
| XLcr-527 | gatttagactaccccaaaaacgaaggggactaaaacAGACACCTAGTCATGATTGCATCACAAC | X | 500 |
| XLcr-528 | gatttagactaccccaaaaacgaaggggactaaaacCAGCTAGACACCTAGTCATGATTGCATC | X | 501 |
| XLcr-529 | gatttagactaccccaaaaacgaaggggactaaaacACCTACAAAGCAACCATGATCTGTATTG | 502 | |
| XLcr-530 | gatttagactaccccaaaaacgaaggggactaaaacACACGCTTAACAAAGCACTCGTGGACAG | 503 | |
| XLcr-531 | gatttagactaccccaaaaacgaaggggactaaaacACGCTTAACAAAGCACTCGTGGACAGCT | 504 | |
| XLcr-532 | gatttagactaccccaaaaacgaaggggactaaaacTCACAACTAGCTACATGTGCATTACCAT | X | 505 |
| XLcr-533 | gatttagactaccccaaaaacgaaggggactaaaacCACAACTAGCTACATGTGCATTACCATG | 506 | |
| XLcr-534 | gatttagactaccccaaaaacgaaggggactaaaacATTTGATTACGTCTATAATCCGTTTATG | 507 | |
| XLcr-535 | gatttagactaccccaaaaacgaaggggactaaaacGATCATGGTTGCTTTGTAGGTTACCTGT | 508 | |
| XLcr-536 | gatttagactaccccaaaaacgaaggggactaaaacCAGATCATGGTTGCTTTGTAGGTTACCT | 509 | |
| XLcr-537 | gatttagactaccccaaaaacgaaggggactaaaacGCTTTTCCACTGCTTCAGACACTTATGC | 510 | |
| XLcr-538 | gatttagactaccccaaaaacgaaggggactaaaacATTATAGGATATTCAATAGTCCAGTCAA | X | 511 |
| XLcr-539 | gatttagactaccccaaaaacgaaggggactaaaacCCAATTATAGGATATTCAATAGTCCAGT | X | 512 |
| XLcr-540 | gatttagactaccccaaaaacgaaggggactaaaacGCTTTAACAACCATGTGTTGAACCTTTC | X | 513 |
| XLcr-541 | gatttagactaccccaaaaacgaaggggactaaaacGCAGCTTTAACAACCATGTGTTGAACCT | X | 514 |
| XLcr-542 | gatttagactaccccaaaaacgaaggggactaaaacCTTTAACAACCATGTGTTGAACCTTTCT | X | 515 |
| MKW0160 | gatttagactaccccaaaaacgaaggggactaaaacACCTCATGGTCATGTTATGGTTGAGCTG | 516 | |
| MKW0161 | gatttagactaccccaaaaacgaaggggactaaaacCAGCTCAACCATAACATGACCATGAGGT | 517 | |
| MKW0162 | gatttagactaccccaaaaacgaaggggactaaaacTGGTCATGTTATGGTTGAGCTGGTAGCA | 518 | |
| MKW0163 | gatttagactaccccaaaaacgaaggggactaaaacTGCTACCAGCTCAACCATAACATGACCA | 519 | |
| MKW0164 | gatttagactaccccaaaaacgaaggggactaaaacCGAACTGCACCTCATGGTCATGTTATGG | 520 | |
| MKW0165 | gatttagactaccccaaaaacgaaggggactaaaacCCATAACATGACCATGAGGTGCAGTTCG | 521 | |
| crRNA N1-1 | gatttagactaccccaaaaacgaaggggactaaaacGCACCCCGCATTACGTTTGGTGGACCCT | X | 522 |
| crRNA N1-2 | gatttagactaccccaaaaacgaaggggactaaaacAGGGTCCACCAAACGTAATGCGGGGTGC | X | 523 |
| crRNA N2-1 | gatttagactaccccaaaaacgaaggggactaaaacTGCACAATTTGCCCCCAGCGCTTCAGCG | X | 524 |
| crRNA N2-2 | gatttagactaccccaaaaacgaaggggactaaaacCGCTGAAGCGCTGGGGGCAAATTGTGCA | X | 525 |
| crRNA N3-1 | gatttagactaccccaaaaacgaaggggactaaaacATCACATTGGCACCCGCAATCCTGCTAA | 526 | |
| crRNA N3-1 | gatttagactaccccaaaaacgaaggggactaaaacTTAGCAGGATTGCGGGTGCCAATGTGAT | 527 |
某些嚮導物係與LAMP引子組組合測試。將所測試之每一嚮導物分級為4：無偵測；3：較差偵測；2：良好偵測；或1：最佳偵測。嚮導物篩選之結果展示於表24中。
表24
此篩選展示用於偵測SARS-CoV-2之存在之LAMP引子及嚮導多核苷酸之獨特組的經驗鑑別。
實例 7 ：交叉反應性研究
| 目標／引子組 | crRNA | 3000 個拷貝 | 30 個拷貝 | NTC | S/N | 等級 | |||
| B3 (orf1ab) | XLcr-507 | 381560 | 606255 | 751128 | 479722 | 11912 | 11033 | 53.6435 | 1 |
| B3 (orf1ab) | XLcr-506 | 159355 | 242958 | 362670 | 283619 | 12702 | 12853 | 25.2901 | 2 |
| B3 (orf1ab) | XLcr-505 | 84626 | 27520 | 13134 | 12996 | 11661 | 9565 | 1.23104 | 4 |
| B5 (orf1ab) | XLcr-509 | 596771 | 655653 | 626136 | 700660 | 11049 | 11547 | 58.7182 | 1 |
| B5 (orf1ab) | XLcr-510 | 500593 | 583394 | 624045 | 612371 | 13989 | 13380 | 45.1758 | 2 |
| B5 (orf1ab) | XLcr-512 | 38997 | 50342 | 79652 | 133126 | 12817 | 12506 | 8.40256 | 3 |
| CN-4B7 (N) | MKW0307 | 193718 | 158289 | 163775 | 172492 | 5365 | 5149 | 31.9828 | 2 |
| B5 (orf1ab) | XLcr-511 | 12617 | 15145 | 19248 | 27464 | 11217 | 11329 | 2.07185 | 4 |
| HK5 (orf1ab) | XLcr-530 | 808713 | 702139 | 727598 | 727101 | 8336 | 8819 | 84.7974 | 1 |
| HK5 (orf1ab) | XLcr-531 | 743506 | 735770 | 671386 | 575016 | 9777 | 8956 | 66.5351 | 2 |
| HK5 (orf1ab) | XLcr-529 | 1019348 | 581204 | 750566 | 660271 | 16473 | 16749 | 42.4669 | 2 |
| HK7-B7 (orf1ab) | XLcr-534 | 788340 | 744356 | 666083 | 695888 | 12366 | 11219 | 57.7473 | 2 |
| HK7-F7 (orf1ab) | XLcr-535 | 866522 | 757465 | 678074 | 730362 | 9309 | 8150 | 80.6711 | 1 |
| CN-4B7 (N) | MKW0306 | 358246 | 318146 | 278357 | 29721 | 5229 | 5164 | 29.6428 | 2 |
| HK7-F7 (orf1ab) | XLcr-536 | 949197 | 885495 | 786778 | 740809 | 10180 | 9335 | 78.2776 | 1 |
| CN-4B7 (N) | MKW0304 | 117477 | 132281 | 132603 | 126557 | 5861 | 5830 | 22.1675 | 2 |
| HK7-F7 (orf1ab) | XLcr-534 | 960662 | 817735 | 779298 | 689392 | 13810 | 12804 | 55.1849 | 2 |
| HK7-F7 (orf1ab) | XLcr-537 | 864567 | 752316 | 755534 | 679622 | 18715 | 19172 | 37.8799 | 2 |
| CN-4B7 (N) | MKW0303 | 112354 | 102155 | 71727 | 93316 | 5624 | 5472 | 14.8741 | 3 |
| CN-4F7 (N) | MKW0304 | 235526 | 220675 | 237769 | 251256 | 5856 | 5720 | 42.2447 | 2 |
| CN-4F7 (N) | MKW0306 | 228295 | 211651 | 132373 | 166404 | 5417 | 5155 | 28.2612 | 2 |
| CN-4F7 (N) | MKW0303 | 76253 | 57912 | 66575 | 71504 | 5846 | 5543 | 12.1239 | 3 |
| CN-4F7 (N) | MKW0307 | 244513 | 239678 | 238032 | 293421 | 73770 | 5403 | 6.71255 | 3 |
| JP13 (orf1ab) | MKW0162 | 776366 | 681657 | 9653 | 667011 | 9754 | 9637 | 34.8958 | 2 |
| JP13 (orf1ab) | MKW0160 | 804053 | 860405 | 16386 | 686270 | 13490 | 12905 | 26.6208 | 2 |
| JP2 (orf1ab) | MKW0163 | 785006 | 669887 | 749394 | 698177 | 9265 | 9736 | 76.1839 | 1 |
| CN-5B7 (N) | MKW0303 | 54150 | 42947 | 42340 | 45260 | 5195 | 33100 | 2.2875 | 4 |
| JP2 (orf1ab) | MKW0162 | 900163 | 796992 | 807423 | 792384 | 15817 | 13983 | 53.6848 | 2 |
| CN-5B7 (N) | MKW0306 | 356470 | 342436 | 317549 | 322836 | 5349 | 322337 | 1.95426 | 4 |
| CN-5F7 (N) | MKW0306 | 322802 | 302853 | 294700 | 305699 | 5273 | 5060 | 58.105 | 2 |
| CN-5F7 (N) | MKW0303 | 24608 | 22195 | 25378 | 20977 | 5448 | 5339 | 4.2973 | 3 |
| JP2 (orf1ab) | MKW0160 | 615770 | 445162 | 405453 | 660643 | 12836 | 14247 | 39.364 | 2 |
| N2-3 (N) | MKW0301 | 139665 | 86572 | 87309 | 67359 | 5199 | 59696 | 2.38337 | 4 |
| N2-3 (N) | MKW0300 | 194311 | 185570 | 154457 | 170884 | 5926 | 145851 | 2.14355 | 4 |
| N2-8 (N) | MKW0300 | 309406 | 218624 | 176785 | 232912 | 6234 | 6268 | 32.7705 | 2 |
| JP2 (orf1ab) | MKW0161 | 788645 | 713904 | 702131 | 704646 | 35679 | 35324 | 19.8129 | 3 |
| N2-8 (N) | MKW0301 | 122293 | 91069 | 91177 | 88135 | 5602 | 5495 | 16.1593 | 3 |
| N2-8 (N) | MKW0302 | 80342 | 39161 | 49717 | 42488 | 5640 | 5411 | 8.34392 | 3 |
| N2-8 (N) | MKW0299 | 8398 | 7396 | 7883 | 6907 | 4111 | 4682 | 1.68202 | 4 |
| JP5 (orf1ab) | MKW0165 | 696060 | 682256 | 15141 | 719209 | 13834 | 15635 | 24.9194 | 2 |
| N2-8 (N) | MKW0298 | 14876 | 8856 | 7590 | 3456 | 3843 | 4707 | 1.29193 | 4 |
| N2-9 (N) | MKW0300 | 320588 | 267128 | 270173 | 273597 | 6644 | 7594 | 38.1927 | 2 |
| N2-9 (N) | MKW0301 | 205854 | 162351 | 148890 | 198734 | 5822 | 5303 | 31.2498 | 2 |
| JP5 (orf1ab) | MKW0164 | 63738 | 55591 | 11900 | 52679 | 12010 | 11523 | 2.74419 | 4 |
| N2-9 (N) | MKW0299 | 6750 | 6278 | 6013 | 6067 | 5151 | 5076 | 1.18119 | 4 |
| N2-9 (N) | MKW0298 | 5850 | 5314 | 5016 | 5075 | 4809 | 4695 | 1.06176 | 4 |
此實例展示本文所述之方法具有靈敏性及特異性。特定而言，本實例展示本文所述之方法不會因交叉反應性而產生SARS-CoV-2之假陽性偵測。
交叉反應性彙集物：在各自由兩種生物體組成之五種彙集物中測試交叉反應性小組。為產生兩種小組成員彙集物，遵循工作單「交叉反應性計算」中之方案，將每一生物體之原液濃度稀釋於不含核酸酶之水中。彙集物中每一生物體之最終濃度將為2×10
4基因體當量/μL (對於細菌及酵母菌)或2×10
3基因體當量/μL (對於病毒)，最終分析濃度為10
6基因體當量/mL VTM (對於細菌及酵母菌)或10
5基因體當量/mL VTM (對於病毒)。可在研究之前製備彙集物且將其儲存在等於或低於-70℃之溫度下。
樣品：在此研究中測試之每一樣品係藉由以下方式來產生：將10微升彙集之經稀釋生物體原液(上文所述)添加至200微升溶解處理之陰性基質中(例如200微升NM AFTER，添加225微升PureLink™溶解緩衝液及蛋白酶K，且在56℃下培育15分鐘)。然後使用PureLink™病毒DNA/RNA微套組、遵循製造商之說明書提取此人為樣品，最終溶析體積為30微升。使用8微升之此溶析樣品作為Sherlock™ CRISPR SARS-CoV-2套組靶向之兩種SARS-CoV-2分析物(即ORF1ab及N靶分析物，及RNA酶P對照)中之每一者之模板。將測試每一溶析樣品之三個重複等分試樣。
對照： i. 提取對照：在SARS-CoV-2信號不存在下，RNA酶P偵測用作提取對照。
ii. 無模板對照：設置「無輸入RNA」反應作為擴增之陰性對照並測定Cas偵測反應之背景螢光水準。對欲測試之每一LAMP引子組及每一嚮導物實施陰性對照。陰性對照係藉由用等體積之不含核酸酶之水替代LAMP反應中之8微升模板體積來產生。
iii. 陽性對照：將對欲測試之每一ORF1ab及N LAMP引子組及每一ORF1ab及N嚮導物實施用於擴增及偵測SARS-CoV-2靶分析物之陽性對照。陽性對照係藉由用等體積之病毒基因體RNA替代LAMP反應中之8微升模板體積來產生，該病毒基因體RNA係自在Vero細胞中繁殖、在Trizol中穩定且運輸至Sherlock Biosciences之所培養SARS-CoV-2病毒提取。藉由數位PCR量化此病毒原液，且在不含核酸酶之水中稀釋至4800個拷貝/微升之濃度，等分用於單次使用。
每一目標引子及嚮導物組之交叉反應性在此方案下係獨立地測定。產生五種生物體彙集物且用於實施活體外交叉反應性研究。每一生物體彙集物係由兩種生物體之核酸組成。樣品係藉由向SARS-CoV-2陰性基質摻入以臨床相關濃度自兩種生物體提取之核酸之彙集的量化原液來產生。使用Sherlock™ CRISPR SARS-CoV-2套組測試每一生物體彙集物之三個重複等分試樣。
生物體彙集物產生：製備下表25中所述之量化之生物體原液彙集物。在添加225微升之PureLink™溶解緩衝液/蛋白酶K且在56℃下培育15分鐘後，將10微升之每一彙集的量化生物體原液摻入200微升之陰性基質中。
| 表 25 ：生物體原液彙集物濃度生物體彙集物 | 生物體 | 來源 | 基因體拷貝 /ul 彙集的生物體原液 | 基因體拷貝 /mL 人為臨床樣品 |
| 1 | 人類冠狀病毒229E | ATCC® VR-740D | 2.0 × 10 3 | 1.0 × 10 5 |
| 人類冠狀病毒OC43 | ATCC® VR-1558D | 2.0 × 10 3 | 1.0 × 10 5 | |
| 2 | 人類冠狀病毒HKU1 | ATCC® VR-3262SD | 2.0 × 10 3 | 1.0 × 10 5 |
| 人類冠狀病毒NL63 | ATCC-3263SD | 2.0 × 10 3 | 1.0 × 10 5 | |
| 3 | A型流行性感冒病毒 | VR-95DQ | 2.0 × 10 3 | 1.0 × 10 5 |
| B型流行性感冒病毒 | VR-1885DQ | 2.0 × 10 3 | 1.0 × 10 5 | |
| 4 | 呼吸道融合病毒 | ATCC® VR-1580DQ | 2.0 × 10 3 | 1.0 × 10 5 |
| 綠膿桿菌 (Pseudomonas aeruginosa) | ATCC® 27853D-5 | 2.0 × 10 4 | 1.0 × 10 6 | |
| 5 | 表皮葡萄球菌 (Staphylococcus epidermis) | ATCC® 12228D-5 | 2.0 × 10 4 | 1.0 × 10 6 |
| 白色念珠菌 (Candida albicans) | ATCC® 10231D-5 | 2.0 × 10 4 | 1.0 × 10 6 |
實施LAMP反應。對於每一所提取樣品，針對三個引子組中之每一者實施一次LAMP反應。另外，如所述包括用於偵測SARS-CoV-2靶之陽性對照(由先前提取之4800 cp/μL之病毒RNA組成)。對三種LAMP引子組及Cas反應中之每一者實施一次陰性對照(由不含核酸酶之水而非模板組成，如所述)。
測試樣品結果之解釋：1. 靶(N、Orf1ab、RNA酶P)解釋：若Cas信號之T10讀數與目標之有效陰性對照(「未添加RNA」)相比增加≥5倍，則樣品視為對該目標呈陽性。SARS-CoV-2 (COVID-19)陽性結果解釋：若在T10處，對於SARS-CoV-2目標分析物(即N或ORF1ab)中之一或多者，人為樣品之螢光Cas信號之T10讀數係有效陰性對照之螢光Cas信號的≥5倍，則樣品對COVID-19呈陽性。SARS-CoV-2 (COVID-19)陰性結果解釋：若在T10處：a. 對於兩種SARS-CoV-2目標分析物，人為樣品之螢光信號比有效陰性對照信號大不到5倍， b. 且RNA酶P信號為陽性(在T10讀取時，RNA酶P螢光信號比有效陰性對照信號大至少5倍)，則樣品對COVID-19呈陰性。4. 無效結果解釋：若在T10讀取時：a. 在T10讀取時對於SARS-CoV-2 (N及ORF1ab)靶分析物，人為樣品之螢光信號比有效陰性對照信號大不到5倍，b. 且在T10讀取時，RNA酶P信號比有效陰性對照信號大不到5倍，則樣本係無效的。具有無效測試結果之任一樣品可自提取步驟開始再測試。
統計/分析方法、樣本大小及驗收準則：若生物體彙集物之0/3個重複對兩種SARS-CoV-2靶呈陽性，則稱該彙集物中之所有生物體不顯示與Sherlock™ CRISPR SARS-CoV-2套組之交叉反應性。a. 無效樣品不包括在結果分析內且再測試。b. 在對彙集物之任一重複偵測到一或多種SARS-CoV-2目標之陽性信號的情況下，遵循上文針對測試生物體彙集物所概述之方案使用三個(n=3)重複個別地測試該彙集物之每一生物體。i. 稱顯示0/3個重複具有兩種SARS-CoV-2目標分析物之陽性偵測之生物體不顯示與Sherlock™ CRISPR SARS-CoV-2套組之交叉反應性。ii. 稱顯示N=1至3個重複具有任一SARS-CoV-2目標之陽性偵測之生物體可能與Sherlock™ CRISPR SARS-CoV-2套組交叉反應。可以一式三份測試「反應性」生物體之連續稀釋物直至0/3個重複對SARS-CoV-2偵測呈陰性。
針對基於疾病盛行率、疾病風險、與分析特異性目標之同源性及與SARS-CoV-2基因體之同源性選擇之呼吸道高風險致病性生物體實施濕式測試以確認電腦分析之結果。使用Sherlock™ CRISPR SARS-CoV-2套組藉由將稀釋之生物體原液摻入溶解處理之彙集的鼻咽拭子基質中以一式三份測試下文鑑別出之每一生物體。所有重複對SARS-CoV-2呈陰性。
實例 8 ：彙集的唾液樣品之偵測極限測試
| 生物體 | ATCC 目錄號 | 濃度 | ORF1ab | N | RNA 酶 P |
| 人類冠狀病毒 229E | ATCC® VR-740D | 1 × 10 5個拷貝/mL | 0/3 | 0/3 | 3/3 |
| 人類冠狀病毒 OC43 | ATCC® VR-1558D | 1 × 10 5個拷貝/mL | 0/3 | 0/3 | 3/3 |
| 人類冠狀病毒 HKU1 | ATCC® VR-3262SD | 1 × 10 5個拷貝/mL | 0/3 | 0/3 | 3/3 |
| 人類冠狀病毒 NL63 | ATCC® 3263SD | 1 × 10 5個拷貝/mL | 0/3 | 0/3 | 3/3 |
| A 型流行性感冒病毒 | VR-95DQ | 1 × 10 5個拷貝/mL | 0/3 | 0/3 | 3/3 |
| B 型流行性感冒病毒 | VR-1885DQ | 1 × 10 5個拷貝/mL | 0/3 | 0/3 | 3/3 |
| 呼吸道融合病毒 | ATCC® VR-1580DQ | 1 × 10 5個拷貝/mL | 0/3 | 0/3 | 3/3 |
| 綠膿桿菌 (Pseudomonas aeruginosa) | ATCC® 27853D-5 | 1 × 10 6個拷貝/mL | 0/3 | 0/3 | 3/3 |
| 表皮葡萄球菌 (Staphylococcus epidermis) | ATCC® 12228D-5 | 1 × 10 6個拷貝/mL | 0/3 | 0/3 | 3/3 |
| 白色念珠菌 (Candida albicans) | ATCC® 10231D-5 | 1 × 10 6個拷貝/mL | 0/3 | 0/3 | 3/3 |
本實例闡述使用唾液樣品測定本文所述SARS-CoV-2診斷之偵測極限之測試。
為使用本文所述之診斷方法測試唾液之偵測極限，將彙集的人類唾液樣品1:1添加至Zymo DNA/RNA唾液套組(R1210-1)中。然後向200μl彙集的唾液摻入SARS-CoV-2陽性對照(SeraCare 0505-129)。然後使用如本文所闡述之Purelink提取套組自陽性對照摻入之唾液樣品提取RNA且溶析於30 ul中。表26展示自唾液提取之SARS-CoV-2之靈敏性偵測。
表26
| 唾液 /Zymo 防腐劑 | |||
| 樣品濃度 | N | Orf | Sherlock 陽性 |
| 12 | 3/3 | 3/3 | 3/3 |
| 4 | 3/3 | 3/3 | 3/3 |
| 2 | 3/3 | 3/3 | 3/3 |
| 1.5 | 5/6 | 6/6 | 6/6 |
| 1 | 3/3 | 3/3 | 3/3 |
| 0.75 | 6/6 | 4/6 | 6/6 |
| 0.375 | 1/3 | 2/3 | 2/3 |
| 0.1875 | 0/3 | 1/3 | 1/3 |
| 0 | 0/7 | 0/7 | 0/7 |
為進一步證實本文所述之分析偵測唾液中之SARS-CoV-2之能力，對未提取RNA之唾液測試本文所述之診斷方法及組成物。將摻入SARS-CoV-2陽性對照之彙集的唾液與快速提取DNA緩衝液(15 ul快速提取DNA緩衝液對15 ul唾液 + SeraCare陽性對照) 1:1混合，在65℃下加熱6 min，在98℃下加熱3 min，且冷卻至4℃，或將3 ul蛋白酶K及12 ul H2O添加至15 ul唾液+防腐劑+Seracare中，然後在55℃下加熱15 min，在98℃下加熱3 min，且冷卻至4℃。表27及表28展示自唾液提取之SARS-CoV-2之靈敏性偵測。
表27
表28
實例 9 ：自動化工作流程
| 快速提取 DNA | |||
| N | Orf | ||
| 50 cp/ul | 200 cp/rxn | 3/3 | 2/3 |
| 25 cp/ul | 100 cp/rxn | 2/3 | 3/3 |
| 5 cp/ul | 20 cp/rxn | 0/3 | 1/3 |
| 0 | 0 | 0/1 | 0/1 |
| 加熱 + 蛋白酶 K | |||
| N | Orf | ||
| 50 cp/ul | 200 cp/rxn | 3/3 | 1/3 |
| 25 cp/ul | 100 cp/rxn | 3/3 | 1/3 |
| 5 cp/ul | 20 cp/rxn | 2/3 | 1/3 |
| 0 | 0 | 0/1 | 0/1 |
| 僅加熱 | |||
| N | Orf | ||
| 50 cp/ul | 400 cp/rxn | 3/3 | 1/3 |
| 25 cp/ul | 200 cp/rxn | 3/3 | 0/3 |
| 5 cp/ul | 40 cp/rxn | 0/3 | 0/3 |
| 0 | 0 | 0/1 | 0/1 |
| Sherlock 陽性 | ||
| 樣品濃度，拷貝 /ul | 快速提取 DNA | 加熱 + 蛋白酶 K |
| 50 | 3/3 | 3/3 |
| 25 | 3/3 | 3/3 |
| 5 | 1/3 | 2/3 |
| 0 | 0/1 | 0/1 |
如本文所述，本實例展示，可組合本文之診斷分析之步驟以改良工作流程之速度、準確性及容易性。圖11展示其中在單一容器中組合且相繼實施某些步驟之有效工作流程。在此例示性工作流程中，製備擴增反應物(例如LAMP) (1)，然後等分至384孔板(2)。在擴增反應(3)後，製備CRISPR/Cas附帶活性分析(4)且等分至同一384孔板用於CRISPR/Cas附帶活性之活化(5)及相關信號之偵測(6)。
將在本文所揭示之自動化製程中分析之每一樣品以一式兩份平鋪於384孔板中。將7 µL溶解溶液(例如蛋白酶K或快速提取)添加至384孔板之每孔中。隨後，將7 µL樣品添加至384孔板之每孔中。將板在55℃下培育15 min，然後在98℃下培育3分鐘。將8 µL之LAMP擴增試劑添加至每孔中。兩個一式兩份樣品中之一者接受SARS-Cov-2 LAMP擴增試劑且其餘一份接受對照LAMP擴增試劑。將20 µL礦物油添加至384孔板之每孔中。將板在61℃下培育40分鐘。將5 µL之SARS-CoV-2 Cas偵測試劑(參見「目標CRISPR Cas主混合物配方」)添加至含有SARS-CoV-2目標之孔中，且將5 µL對照Cas偵測試劑添加至含有對照目標之孔中。在螢光讀板儀上在37℃下使用分別為485奈米及528奈米之激發-發射完成信號偵測。應注意，在LAMP反應後，不將板冷卻至4℃而是室溫。
| 目標 CRISPR Cas 主混合物配方 | ||
| 試劑 | 每反應之體積 | 總體積 |
| 預混合物 | 7.5 µL | 7.5 µL × (12 + 1)= |
| crRNA N | 2.25 uL | 2.25 uL × (12+1)= |
| crRNA O | 2.25 µL | 2.25 µL × (12+1)= |
| MgCl2 | 0.23 µL | 0.23 µL × (12+1)= |
| 總體積 | 12.23 µL | 12.23 µL × (12+1)= |
圖12展示，在單一板上組合實施擴增、CRISPR/cas活化及偵測產生更簡單之工作流程以及可靠的結果。應注意，將偵測N之cRNA及偵測ORF1ab之cRNA組合於單一偵測反應中產生SARS-CoV-2之靈敏性偵測。圖13展示在組合工作流程中起始偵測後10分鐘及20分鐘測定之RFU之間的顯著差異，此在別處未觀察到。
另外，圖14顯示使用本文所述之方法提取且使用組合工作流程(「新工作流程」)分析之含有SARS-CoV-2之唾液樣品之比較。圖15顯示本文所述方法之靈敏度之進一步確認。使用本文所述之384孔板工作流程分析唾液樣品(50個、25個、10個、5個及0個拷貝/µL樣品之10 µL彙集的唾液)。簡言之，將蛋白酶K (PK)或快速提取(QE)添加至樣品中且在65℃下加熱6 min並在98℃下加熱3 min。然後添加LAMP主混合物，在61℃下加熱40 mi。然後添加Cas主混合物且將板在讀板儀上在37℃下培育，同時偵測信號。應注意，組合工作流程提供SARS-CoV-2之靈敏性偵測。
實例 10 ：患者鼻咽拭子之 SARS-CoV-2 偵測
如本文所述，本實例進一步展示SHERLOCK CRISPR SARS-CoV-2套組之靈敏度及特異性。本實例闡述來自鼻咽拭子之總共20個COVID-19患者樣品(10個陽性及10個陰性)之SARS-CoV-2之偵測。在先前經驗證之RT-qPCR分析(CDC，Abbott，m2000)上測試所選COVID-19患者樣品。基於寬範圍之循環臨限值(Ct) (包含低(µ = 7.11)、中(µ = 17.2)及高(µ = 27.9) Ct值之平均值)來選擇陽性樣品。使用EZ1高級系統(Qiagen)實施鼻咽拭子患者樣品之核酸提取。遵循SHERLOCK CRISPR SARS-CoV-2套組說明書，使所提取材料經受反轉錄酶環介導之擴增。將擴增產物與特異性針對SARS-CoV-2目標之CRISPR嚮導RNA複合之Cas13a酶一起培育。在微量讀板儀(BioTek)上以2.5分鐘間隔持續總共10分鐘獲取所裂解報導子分子之螢光讀出。將相對螢光單位比率之數據輸出標準化至無模板對照。所有20個COVID-19患者樣品皆經正確診斷，準確性高達100%。所有對照(包括RNA酶P)皆顯示預期發現，即 對經稀釋陽性對照分離物(BEI)偵測到5500個拷貝/µl。對於COVID-19陽性樣品，對於N及ORF1ab基因目標，標準化比率分別介於16.45至49.17及33.82至48.15範圍內。對於N及ORF1ab基因目標，陰性樣品之螢光比率分別介於0.54至1.28及0.84至4.93範圍內。所測定比率足夠大於或小於預先建立之螢光讀數輸出之5倍變化，排除任何臨界結果之解釋。
實例 11 ：即時多工 SARS-CoV-2 偵測
本實例確認，如本文所闡述之熱穩定Cas酶允許在單一反應容器中實施多個反應步驟(例如「一鍋式」)。使用熱穩定Cas會減少或消除某些處理及/或轉移步驟。本實例展示，使用熱穩定Cas，可在單一容器中實施除核酸分離外之所有反應步驟。
本實例展示，使用本文所述與LAMP相容之新熱穩定Cas12蛋白(SLK-9 (亦可互換地稱為rs9；SEQ ID NO: 15)提供改良之實時SHERLOCK系統(RT-SHERLOCK)，其將工作流程自兩步工作流程顯著簡化至單一反應，同時提供即時信號讀出。另外，兩種不同CRISPR-Cas系統之組合(SLK-9；SEQ ID NO:15及AacCas12b；SEQ ID NO: 3)生成第一個基於CRISPR之即時多工診斷平台(Duplex Aac/rs9-cas12實時Sherlock; DARTS)，其能夠同時偵測SARS-CoV-2 RNA及人類RNA酶P內部對照。
RT-SHERLOCK及DARTS之一步工作流程係藉由將經提取或未經提取之COVID-19患者前鼻拭子或唾液樣品添加至含有RT-SHERLOCK或DARTS反應混合物之反應管中、然後即時監測螢光信號變化來實施。藉由Purelink提取套組根據其方案純化所提取樣品且溶析至水中。藉由添加蛋白酶K及RNAecure (65℃ 15 min，95℃ 10 min)使未經提取之樣品簡單地熱溶解。例示性DARTS設計(DARTSv1)顯示於表29中，其中DARTSv1使用AacCas12b系統來偵測N基因且使用rs9系統來偵測RNA酶P (RP)內部對照。圖16顯示實驗資料，其展示雙工系統同時偵測SARS-CoV-2及RP二者之能力。
表29
| DARTS 設計 | Aac Cas12b | Rs9 cas12a |
| 目標 | SARS-CoV-2 N基因 | RNA酶P內部對照 |
| 報導子 | 聚T7-FAM | 聚T7-FAM 聚C7-TexasRed |
| 螢光通道 | x1-m1 | x1-m1 x4-m4 |
| 溫度 | 56℃ | |
| 嚮導RNA | 嚮導1 (XL-A226) | 嚮導2 (XL-374) |
| 引子組 | CNFB | RP |
DARTSv1之偵測極限之初始評估展示LOD為14至28 cp/μl (圖17)。
另一例示性DARTS平台(DARTs v2)含有RT-LAMP反應混合物以提供足以用於雙工LAMP擴增之試劑、用於N及RP之兩個LAMP引子組、含有靶向N之crRNA之SLK9酶、含有靶向RP之crRNA之AacCas酶、FAM淬滅劑修飾之T報導基因及HEX淬滅劑修飾之C報導基因(圖18及圖19)。DARTS係多工實時CRIPSR診斷平台之第一次展示。為使用DARTS分析，使用者之唯一操作步驟係將樣品添加至DARTS反應物中且將反應物置於具有螢光監測器及溫度控制器之裝置(例如讀板儀或qPCR儀器)中。由於Aac系統之較弱熱穩定性，在56℃下實施例示性DARTS分析，此溫度低於最佳SLK9反應溫度。在反應中，當將樣品添加至DARTS中時，N或RP藉由相應LAMP引子組擴增，其後為相應Cas酶活化。當SLK9活化時，其將裂解C及T報導基因二者，從而發出FAM及HEX螢光。當Aac活化時，其僅裂解T報導基因，僅發出FAM螢光。分析結果解釋遵循簡單且直觀的「雙線意指陽性，單線意指陰性」規則：陽性樣品在FAM及HEX通道中顯示開(ON)信號，陰性樣品在FAM通道中顯示開信號且在HEX通道中顯示關(OFF)信號。若在FAM通道或兩個通道中可見關信號，則分析測定為無效。發現DARTSv2之LOD為7至14 cp/μl (圖19)。
對具或不具提取之總共60個陽性及陰性患者樣品評估RT-SHERLOCK及DARTS分析，且與傳統RT-PCR達成98%一致性(在總共60個中正確地鑑別出58個；圖20及圖21)。未觀察到假陽性。結果之時間可快至12分鐘，此端視患者樣品及所用提取方法而定。RT-SHERLOCK分析型偵測極限為0.5個拷貝/uL (對於經提取之樣品)及10至20個拷貝/μL (對於未經提取之樣品)，此端視樣品類型而定。DARTS分析型偵測極限為10個拷貝/uL (對於經提取之樣品)及60個拷貝/μL (對於未經提取之樣品)。
藉由將10 µL或5 µL預處理之臨床樣品直接添加至DARTS反應混合物中、且然後在QuantStudio 5 qPCR儀器上在56℃下量測螢光讀出來實施臨床樣品之例示性DARTS偵測。例示性DARTS反應混合物顯示於表11-1中。
表11-1：
| DARTS反應混合物 | 原液濃度 | 每反應之量(uL) | |
| 所提取樣品 | 未經提取之樣品 | ||
| WarmStart LAMP反應混合物(NEB) | 2× | 25 | 25 |
| CNFB引子混合物 | - | 2.2 | 2.2 |
| RPFB引子混合物 | - | 2.2 | 2.2 |
| Aac Cas12b酶 | 2 mg/mL | 1.2 | 1.2 |
| SLK9 Cas12a酶 | 2.83 mg/mL | 0.65 | 0.65 |
| 用於RP之AacCas12b crRNA | 10 uM | 2.6 | 2.6 |
| 用於N之SLK9 Cas12a crRNA | 10 uM | 0.6 | 0.6 |
| 報導子C | 20 uM | 0.5 | 0.5 |
| 報導子T | 20 uM | 0.5 | 0.5 |
| 水 | - | 4.55 | 9.55 |
| 樣品 | - | 10 | 5 |
| 總體積 | - | 50 | 50 |
例示性引子混合物顯示於表11-2中。
表11-2：
| 引子混合物 | 原液濃度 | 量(uL) |
| F3 | 100 uM | 10 |
| B3 | 100 uM | 10 |
| FIP | 100 uM | 80 |
| BIP | 100 uM | 80 |
| LF | 100 uM | 20 |
| LB | 100 uM | 20 |
| 總體積 | - | 220 |
用於DARTS反應之例示性序列顯示於表11-3中。
表11-3：
| 名稱 | 序列 |
| 用於RP之Aac Cas12b crRNA | GTCTAGAGGACAGAATTTTTCAACGGGTGTGCCAATGGCCACTTTCCAGGTGGCAAAGCCCGTTGAGCTTCTCAAATCTGAGAAGTGGCACAGTGGAGGAGTGTCTTTTCAA (SEQ ID NO: 742) |
| 用於N之SLK9 Cas12a crRNA | AAUUUCUACUAUUGUAGAUCUCCUGCUAGAAUGGCUGGCAAUGGC (SEQ ID NO: 743) |
| CNFB引子：F3 | GCTTCTACGCAGAAGGGA (SEQ ID NO: 744) |
| CNFB引子：B3 | GTGACAGTTTGGCCTTGT (SEQ ID NO: 745) |
| CNFB引子：FIP | CTACTGCTGCCTGGAGTTGAATTCCTCTTCTCGTTCCTCATC (SEQ ID NO: 746) |
| CNFB引子：BIP | GCTTTGCTGCTGCTTGACAGTGTTGTTGGCCTTTACCA (SEQ ID NO: 747) |
| CNFB引子：LF | CTTGAACTGTTGCGACTACGT (SEQ ID NO: 748) |
| CNFB引子：LB | ATTGAACCAGCTTGAGAGCAAA (SEQ ID NO: 749) |
| RPFB引子：F3 | TTGATGAGCTGGAGCCA (SEQ ID NO: 750) |
| RPFB引子：B3 | CACCCTCAATGCAGAGTC (SEQ ID NO: 751) |
| RPFB引子：FIP | GTGTGACCCTGAAGACTCGGTTTTAGCCACTGACTCGGATC (SEQ ID NO: 752) |
| RPFB引子：BIP | CCTCCGTGATATGGCTCTTCGTTTTTTTCTTACATGGCTCTGGTC (SEQ ID NO: 753) |
| RPFB引子：LF | ATGTGGATGGCTGAGTTGTT (SEQ ID NO: 754) |
| RPFB引子：LB | GGCATGCTGAGTACTGGACCTC (SEQ ID NO: 755) |
| 報導基因C | /5TexRd-XN/CCCCCCC/3IAbRQSp/ (SEQ ID NO: 756) |
| 報導基因T | /56-FAM/TTTTTTT/3IABkFQ/ (SEQ ID NO: 757) |
基於新穎熱穩定cas12a酶(SLK-9)之RT-SHERLOCK及DARTS分析可達成自臨床樣品偵測SARS-CoV-2 RNA之PCR樣高靈敏度及特異性。工作流程係簡單、快速、高通量且自動化相容的。該兩種分析具有縮短當前COVID-19診斷分析的周轉時間並改良所有實驗室的通量、從而在不犧牲效能的情況下提高其測試能力之潛能。
等效內容
熟習此項技術者僅使用常規實驗即可意識到或能夠確定本文所述發明之特定實施例之許多等效內容。本發明之範圍不欲限於上述說明書，而是如以下申請專利範圍中所闡述：
無
圖1呈現SARS-CoV-2；SARS-CoV；及MERS-CoV之開放閱讀框。
圖2提供用於偵測本文所述之SARS-CoV-2之例示性工作流程。
圖3：30 cp/μL之靶1 (orf1ab)及6 cp/μL之靶2 (N)以及陽性對照(5000 cp/μL)及陰性對照(0 cp/μL)之樣品偵測之三個重複(n = 3)之經繪圖平均值。
圖4：靶1 (orf1ab)及靶2 (N)之個別重複之15分鐘(T15/T0)時之信號對背景比率線性尺度繪圖。
圖5：靶1 (orf1ab)及靶2 (N)之個別重複之10分鐘(T10/T0)時之信號對背景比率線性尺度繪圖。
圖6：靶1 (orf1ab)及靶2 (N)之個別重複之15分鐘(T15/T0)時之信號對背景比率對數尺度繪圖。
圖7：靶1 (orf1ab)及靶2 (N)之個別重複之10分鐘(T10/T0)時之信號對背景比率對數尺度繪圖。
圖8：45 cp/μL之靶1 (orf1ab)及9 cp/μL之靶2 (N)以及陽性對照(5000 cp/μL)及陰性對照(0 cp/μL)之樣品偵測之20個重複(n = 20)之經繪圖平均值。
圖9：靶1 (orf1ab)及靶2 (N)之個別重複之15分鐘(T15/T0)時之信號對背景比率對數尺度繪圖。若樣品之S:B比率係＜5.0，則結果視為陰性。
圖10：靶1 (orf1ab)及靶2 (N)之個別重複之10分鐘(T10/T0)時之信號對背景比率對數尺度繪圖。若樣品之S:B比率係＜5.0，則結果視為陰性。
圖11顯示如本文所闡述之組合工作流程之例示性工作流程。
圖12顯示使用如本文所闡述之組合「自動化」工作流程偵測SARS-CoV-2 (N及Orf1ab (「O」)之結果。
圖13顯示組合工作流程相對於標準工作流程之RFU之比較。
圖14顯示使用本文所述之方法提取且使用組合工作流程(「新工作流程」)分析之含SARS-CoV-2之唾液樣品之比較。
圖15進一步展示利用使用本文所述之方法提取且使用組合工作流程分析之含SARS-CoV-2之唾液樣品偵測之靈敏度。
圖16展示雙工系統(DARTSv1)同時偵測SARS-CoV-2及RP二者之能力。
圖17顯示DARTSv1之偵測極限之評估。
圖18展示雙工系統(DARTSv2)同時偵測SARS-CoV-2及RP二者之能力。
圖19顯示DARTSv2之偵測極限之評估。
圖20顯示關於未經提取之NP拭子臨床樣品DARTSv2與PCR SARS-CoV-2偵測結果之一致性。
圖21顯示關於所提取之臨床樣品DARTSv2與PCR SARS-CoV-2偵測結果之一致性。
Claims (60)
- 一種組成物，其包含 (i) 嚮導多核苷酸，其具有包含選自由以下組成之群組之crRNA之核苷酸序列：SEQ ID NO.: 77；SEQ ID NO.: 84；SEQ ID NO.: 91；及 (ii) CRISPR/Cas酶。
- 如請求項1之組成物，其中該CRISPR/Cas酶係V型CRISPR/Cas酶。
- 如請求項1之組成物，其中該CRISPR/Cas酶係VI型CRISPR/Cas酶。
- 如請求項1之組成物，其中該CRISPR/Cas酶係熱穩定CRISPR/Cas酶。
- 如請求項1之組成物，其中該CRISPR/Cas酶係Cas13 CRISPR/Cas酶。
- 一種組成物，其包含： 反轉錄酶； DNA聚合酶； 核苷酸 RNA； 選自由以下組成之群組之引子組： (a)：orf1ab-F3 TGAAAATAGGACCTGAGCG (SEQ ID NO. 72) orf1ab-B3 ACACCTAGTCATGATTGCA (SEQ ID NO. 73) orf1ab-FIP CCAATAGAATGATGCCAACAGGCGATAGACGTGCCACATGC (SEQ ID NO. 74) orf1ab-BIP GATTGATGTTCAACAATGGGGTTTCATTACCATGGACTTGACAAT (SEQ ID NO. 75) orf1ab-LF-T7 gaaatTAATACGACTCACTATAGGGAAGTGTCTGAAGCAGTGGAAAA(SEQ ID NO. 76)； (b)： N-5F3 GCTTCTACGCAGAAGGGA (SEQ ID NO. 78) N-5B3 GTGACAGTTTGGCCTTGT (SEQ ID NO. 79) N-5FIP TACTGCTGCCTGGAGTTGAATTCCTCTTCTCGTTCCTCATC (SEQ ID NO. 80) N-5BIP GCTTTGCTGCTGCTTGACAGTGTTGTTGGCCTTTACCA (SEQ ID NO. 81) N-5LB ATTGAACCAGCTTGAGAGCAAA (SEQ ID NO. 82) N-5LF-T7 gaaatTAATACGACTCACTATAGGGCTTGAACTGTTGCGACTACGT(SEQ ID NO. 83)； (c) RP- F3 TTGATGAGCTGGAGCCA (SEQ ID NO. 85) RP-B3 CACCCTCAATGCAGAGTC (SEQ ID NO. 86) RP-FIP GTGTGACCCTGAAGACTCGGTTTTAGCCACTGACTCGGATC (SEQ ID NO.87) RP-BIP CCTCCGTGATATGGCTCTTCGTTTTTTTCTTACATGGCTCTGGTC (SEQ ID NO. 88) RP-LF ATGTGGATGGCTGAGTTGTT (SEQ ID NO. 89) RP-LB-T7 GAATTAATACGACTCACTATAGGGCATGCTGAGTACTGGACCTC (SEQ ID NO. 90)；及其組合。
- 如請求項6之組成物，其中該RNA係自個體之樣品分離。
- 如請求項7之組成物，其中該個體正暴露於或感染SARS-CoV-2，或懷疑暴露於或感染SARS-CoV-2，或曾暴露於或感染SARS-CoV-2。
- 一種組成物，其包含： 包含啟動子之DNA； 經標記核酸報導子構築體； 核糖核苷三磷酸(rNTP)； RNA聚合酶； CRISPR/Cas酶； 嚮導多核苷酸，其具有包含選自由以下組成之群組之crRNA之核苷酸序列：SEQ ID NO.: 77；SEQ ID NO.: 84；SEQ ID NO.: 91。
- 如請求項9之組成物，其中該啟動子包含pol I、pol II、pol III、T7、T3、Sp6、U6、H1、反轉錄病毒勞斯肉瘤病毒(Rous sarcoma virus，RSV) LTR啟動子、巨細胞病毒(CMV)啟動子、SV40啟動子、二氫葉酸還原酶啟動子、β-肌動蛋白啟動子、磷酸甘油激酶(PGK)啟動子及EF1.α.啟動子。
- 如請求項9之組成物，其中該RNA聚合酶係T7 RNA聚合酶。
- 如請求項9之組成物，其中該CRISPR/Cas酶係V型CRISPR/Cas酶。
- 如請求項9之組成物，其中該CRISPR/Cas酶係VI型CRISPR/Cas酶。
- 如請求項9之組成物，其中該CRISPR/Cas酶係熱穩定CRISPR/Cas酶。
- 如請求項9之組成物，其中該CRISPR/Cas酶係Cas13 CRISPR/Cas酶。
- 如請求項9之組成物，其中該CRISPR/Cas酶展現附帶RNA酶活性。
- 如請求項9之組成物，其中該經標記核酸報導子構築體係RNA。
- 如請求項9之組成物，其中該經標記核酸報導子構築體包含螢光/淬滅劑對。
- 一種偵測SARS-CoV-2目標核酸序列之方法，該方法包括： 使RNA製劑與結合至嚮導多核苷酸之CRISPR/Cas酶接觸，該嚮導多核苷酸具有包含選自由以下組成之群組之crRNA之核苷酸序列：SEQ ID NO.: 77；SEQ ID NO.: 84；SEQ ID NO.: 91； 在rNTP及經標記核酸報導子構築體存在下； 其中該CRISPR/Cas酶對該經標記核酸報導子構築體之裂解產生可偵測信號； 其中該可偵測信號之偵測指示該RNA製劑中存在該SARS-CoV-2目標核酸序列。
- 一種偵測SARS-CoV-2目標核酸序列之方法，該方法包括： (i)自個體獲得樣品 (ii)自該樣品分離核酸 (iii)藉由使該經分離核酸與選自由以下組成之群組之引子組接觸來擴增目標序列： (a)：orf1ab-F3 TGAAAATAGGACCTGAGCG (SEQ ID NO.72) orf1ab-B3 ACACCTAGTCATGATTGCA (SEQ ID NO. 73) orf1ab-FIP CCAATAGAATGATGCCAACAGGCGATAGACGTGCCACATGC (SEQ ID NO. 74) orf1ab-BIP GATTGATGTTCAACAATGGGGTTTCATTACCATGGACTTGACAAT (SEQ ID NO. 75) orf1ab-LF-T7 gaaatTAATACGACTCACTATAGGGAAGTGTCTGAAGCAGTGGAAAA(SEQ ID NO.76)； (b)： N-5F3 GCTTCTACGCAGAAGGGA (SEQ ID NO. 78) N-5B3 GTGACAGTTTGGCCTTGT (SEQ ID NO. 79) N-5FIP TACTGCTGCCTGGAGTTGAATTCCTCTTCTCGTTCCTCATC (SEQ ID NO. 80) N-5BIP GCTTTGCTGCTGCTTGACAGTGTTGTTGGCCTTTACCA (SEQ ID NO. 81) N-5LB ATTGAACCAGCTTGAGAGCAAA (SEQ ID NO. 82) N-5LF-T7 gaaatTAATACGACTCACTATAGGGCTTGAACTGTTGCGACTACGT(SEQ ID NO.83)； (c) RP- F3 TTGATGAGCTGGAGCCA (SEQ ID NO. 85) RP-B3 CACCCTCAATGCAGAGTC (SEQ ID NO. 86) RP-FIP GTGTGACCCTGAAGACTCGGTTTTAGCCACTGACTCGGATC (SEQ ID NO.87) RP-BIP CCTCCGTGATATGGCTCTTCGTTTTTTTCTTACATGGCTCTGGTC (SEQ ID NO. 88) RP-LF ATGTGGATGGCTGAGTTGTT (SEQ ID NO. 89) RP-LB-T7 GAATTAATACGACTCACTATAGGGCATGCTGAGTACTGGACCTC (SEQ ID NO. 90)；及其組合。 反轉錄酶； DNA聚合酶； 核苷酸 (iv)使該等擴增之目標序列與以下接觸： CRISPR/Cas酶； 嚮導多核苷酸，其具有包含選自由以下組成之群組之crRNA之核苷酸序列：SEQ ID NO.: 77；SEQ ID NO.: 84；SEQ ID NO.: 91。 rNTP； 經標記核酸報導子構築體； 其中該CRISPR/Cas酶對該經標記核酸報導子構築體之裂解產生可偵測信號； 其中該可偵測信號之偵測指示存在該SARS-CoV-2目標核酸序列。
- 如請求項20之方法，其中該個體正暴露於或感染SARS-CoV-2，或懷疑暴露於或感染SARS-CoV-2。
- 如請求項20之方法，其中該樣品係生物樣品。
- 如請求項20之方法，其中該樣品包含鼻拭子、鼻咽拭子、口咽拭子、鼻抽吸物、痰、支氣管肺泡灌洗、血液、血清、糞便及唾液。
- 如請求項20之方法，其中該自該樣品分離核酸之步驟包括自該樣品分離RNA。
- 如請求項20之方法，其中該經標記核酸報導子構築體係RNA。
- 如請求項20之方法，其中該經標記核酸報導子構築體包含 螢光/淬滅劑對。
- 如請求項20之方法，其中該可偵測信號係螢光偵測。
- 如請求項20之組成物，其中該CRISPR/Cas酶係V型CRISPR/Cas酶。
- 如請求項20之組成物，其中該CRISPR/Cas酶係VI型CRISPR/Cas酶。
- 如請求項20之組成物，其中該CRISPR/Cas酶係熱穩定CRISPR/Cas酶。
- 如請求項20之組成物，其中該CRISPR/Cas酶係Cas13 CRISPR/Cas酶。
- 如請求項20之組成物，其中該CRISPR/Cas酶展現附帶RNA酶活性。
- 一種引子組，其具有至少一個選自如表20中所列群組之引子。
- 如請求項33之引子組，其中至少一個引子包含RNA聚合酶啟動子。
- 如請求項34之引子組，其中該RNA聚合酶啟動子包含 pol I、pol II、pol III、T7、T3、SP6、U6、H1、反轉錄病毒勞斯肉瘤病毒(RSV) LTR啟動子、巨細胞病毒(CMV)啟動子、SV40啟動子、二氫葉酸還原酶啟動子、β-肌動蛋白啟動子、磷酸甘油激酶(PGK)啟動子及EF1.α.啟動子。
- 如請求項35之引子組，其中該RNA聚合酶啟動子係T7。
- 如請求項34之引子組，組具有至少一個選自表17中所列群組之引子。
- 一種引子組，其包含： orf1ab-F3 TGAAAATAGGACCTGAGCG (SEQ ID NO. 72)； orf1ab-B3 ACACCTAGTCATGATTGCA (SEQ ID NO.73)； orf1ab-FIP CCAATAGAATGATGCCAACAGGCGATAGACGTGCCACATGC (SEQ ID NO.74)； orf1ab-BIP GATTGATGTTCAACAATGGGGTTTCATTACCATGGACTTGACAAT (SEQ ID NO.75)；及 orf1ab-LF-T7 gaaatTAATACGACTCACTATAGGGAAGTGTCTGAAGCAGTGGAAAA(SEQ ID NO.76)。
- 一種引子組，其包含： N-5F3 GCTTCTACGCAGAAGGGA (SEQ ID NO. 78)； N-5B3 GTGACAGTTTGGCCTTGT (SEQ ID NO. 79)； N-5FIP TACTGCTGCCTGGAGTTGAATTCCTCTTCTCGTTCCTCATC (SEQ ID NO.80)； N-5BIP GCTTTGCTGCTGCTTGACAGTGTTGTTGGCCTTTACCA (SEQ ID NO.81)； N-5LB ATTGAACCAGCTTGAGAGCAAA (SEQ ID NO.82)；及 N-5LF-T7 gaaatTAATACGACTCACTATAGGGCTTGAACTGTTGCGACTACGT(SEQ ID NO.83)。
- 一種引子組，其包含： N2-2F3 AACACAAGCTTTCGGCAG (SEQ ID NO.: 347) N2-2B3 TTGGATCTTTGTCATCCAATT(SEQ ID NO.: 348) N2-2FIP CGGCCAATGTTTGTAATCAGTTCCCAGAACAAACCCAAGGAAAT(SEQ ID NO.: 349)； N2-2BIP GTTCTTCGGAATGTCGCGCATGATGGCACCTGTGTAGG(SEQ ID NO.: 350)； N2-2LB TTGGCATGGAAGTCACACCT(SEQ ID NO.: 352)； N2-2LFT7 gaaatTAATACGACTCACTATAGGGTGATTAGTTCCTGGTCCCCAAA (SEQ ID NO.: 528)。
- 一種用於環介導之等溫擴增之引子組，其包含： JpS1-F3 GAGTTGATTTTTGTGGAAAGG(SEQ ID NO.: 248) JpS1-B3 GTTACAAACCAGTGTGTGC (SEQ ID NO.: 249) JpS1-FIP AGGGACATAAGTCACATGCAAGAAGCTATCATCTTATGTCCTTCCCTC JpS1-BIP ACAAGAAAAGAACTTCACAACTGCTTTGAAACAAAGACACCTTCA(SEQ ID NO.: 250) JpS1-LFF7 gaaatTAATACGACTCACTATAGGGACACCATGAGGTGCTGACT (SEQ ID 529)。
- 一種偵測樣品中之SARS-CoV-2目標核酸序列之方法，其包括： 使該樣品之核酸與以下接觸： 如請求項33至41中任一項之引子組； VI型Cas； 至少一種嚮導多核苷酸，其包含能夠結合該目標核酸序列之crRNA；及 經標記核酸報導構築體，其中該VI型Cas展現附帶RNA酶活性，且一旦因存在該目標序列而活化便立即裂解該經標記核酸報導構築體； 偵測來自經標記核酸報導構築體裂解之信號，由此偵測該樣品中之該SARS-CoV-2目標核酸序列。
- 如請求項42之方法，其中該VI型Cas係Cas13。
- 如請求項42之方法，其進一步包括自個體獲得生物樣品。
- 如請求項42之方法，其中該生物樣品包含鼻拭子、鼻咽拭子、口咽拭子、鼻抽吸物、痰、支氣管肺泡灌洗、血液、糞便及唾液樣品。
- 如請求項42之方法，其中該偵測信號之步驟包括偵測螢光、吸光度、光譜、側向流、遷移、化學發光、遷移、電化學偵測。
- 一種表23中所列之嚮導多核苷酸。
- 一種嚮導多核苷酸，其核苷酸序列包含： crRNA gatttagactaccccaaaaacgaaggggactaaaacGATCATGGTTGCTTTGTAGGTTACCTGT (SEQ ID NO.: 77)。
- 一種嚮導多核苷酸，其包含： crRNA gatttagactaccccaaaaacgaaggggactaaaacGGTGATGCTGCTCTTGCTTTGCTGCTGC (SEQ ID NO.: 84)。
- 一種嚮導多核苷酸，其包含： crRNA gatttagactaccccaaaaacgaaggggactaaaacAGTGGAGGAGTGTCTTTTCAATTACTTG (SEQ ID NO.: 91)。
- 一種嚮導多核苷酸，其包含： crRNA-N2-3 CAATTTGCCCCCAGCGCTTCAGCGTTCT。
- 一種嚮導多核苷酸，其包含： JpS1-crRNA-1 AAAGTGTGCTTTTCCATCATGACAAATG。
- 一種嚮導多核苷酸，其包含： JpS1-crRNA-2 GTGTGCTTTTCCATCATGACAAATGGCA。
- 一種偵測樣品之SARS-CoV-2目標核酸序列之方法，其包括： 使核酸與以下接觸： 用於擴增該SARS-CoV-2目標核酸序列之如請求項33至41中任一項之引子組； VI型Cas； 至少一種能夠結合該目標核酸序列之如請求項47至53中任一項之嚮導多核苷酸； 及經標記核酸報導構築體，其中該 VI型Cas展現附帶RNA酶活性，且一旦經該目標序列活化便立即裂解該經標記核酸報導構築體； 偵測來自經標記核酸報導構築體裂解之信號，由此偵測該樣品中之該SARS-CoV-2目標核酸。
- 如請求項54之方法，其中該VI型Cas係Cas13。
- 如請求項54之方法，其進一步包括自個體獲得生物樣品。
- 如請求項54之方法，其中該生物樣品包含鼻拭子、鼻咽拭子、口咽拭子、鼻抽吸物、痰、支氣管肺泡灌洗、血液、糞便及唾液樣品。
- 如請求項54之方法，其中該偵測信號之步驟包括偵測螢光、吸光度、光譜、側向流、遷移、化學發光、遷移、電化學偵測。
- 一種診斷SARS-CoV-2之方法，該方法包括以下步驟： 自個體獲得樣品 自該樣品分離核酸 偵測該經分離核酸中之SARS-CoV-2核酸 其中該偵測步驟包括： 藉由使該經分離核酸與包含以下之擴增試劑接觸來擴增目標序列： orf1ab-F3 TGAAAATAGGACCTGAGCG (SEQ ID NO.72) orf1ab-B3 ACACCTAGTCATGATTGCA (SEQ ID NO. 73) orf1ab-FIP CCAATAGAATGATGCCAACAGGCGATAGACGTGCCACATGC (SEQ ID NO. 74) orf1ab-BIP GATTGATGTTCAACAATGGGGTTTCATTACCATGGACTTGACAAT (SEQ ID NO. 75) orf1ab-LF-T7 gaaatTAATACGACTCACTATAGGGAAGTGTCTGAAGCAGTGGAAAA(SEQ ID NO.76)； N-5F3 GCTTCTACGCAGAAGGGA (SEQ ID NO. 78) N-5B3 GTGACAGTTTGGCCTTGT (SEQ ID NO. 79) N-5FIP TACTGCTGCCTGGAGTTGAATTCCTCTTCTCGTTCCTCATC (SEQ ID NO. 80) N-5BIP GCTTTGCTGCTGCTTGACAGTGTTGTTGGCCTTTACCA (SEQ ID NO. 81) N-5LB ATTGAACCAGCTTGAGAGCAAA (SEQ ID NO. 82) N-5LF-T7 gaaatTAATACGACTCACTATAGGGCTTGAACTGTTGCGACTACGT(SEQ ID NO.83)；及 RP- F3 TTGATGAGCTGGAGCCA (SEQ ID NO. 85) RP-B3 CACCCTCAATGCAGAGTC (SEQ ID NO. 86) RP-FIP GTGTGACCCTGAAGACTCGGTTTTAGCCACTGACTCGGATC (SEQ ID NO.87) RP-BIP CCTCCGTGATATGGCTCTTCGTTTTTTTCTTACATGGCTCTGGTC (SEQ ID NO. 88) RP-LF ATGTGGATGGCTGAGTTGTT (SEQ ID NO. 89) RP-LB-T7 GAATTAATACGACTCACTATAGGGCATGCTGAGTACTGGACCTC (SEQ ID NO.90)； 使該等擴增之目標序列與以下接觸： VI型Cas； 至少一種嚮導多核苷酸，其包含能夠結合該等擴增之目標序列且經設計以與該VI型Cas形成複合物之嚮導序列； 及經標記核酸報導子構築體，其中該VI型Cas展現附帶RNA酶活性，且一旦經該目標序列活化便立即裂解該經標記核酸報導子構築體； 及偵測來自該非目標序列裂解之信號，由此偵測該樣品中之該SARS-CoV-2目標核酸 其中該SARS-CoV-2目標核酸之偵測足以診斷SARS-CoV-2。
- 一種套組，其包含如請求項33至41之引子及如請求項47至53之嚮導物以及實施如請求項54至59之方法之試劑。
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| JP3313358B2 (ja) | 1998-11-09 | 2002-08-12 | 栄研化学株式会社 | 核酸の合成方法 |
| IL149446A0 (en) | 1999-11-08 | 2002-11-10 | Eiken Chemical | Method for synthesizing nucleic acid |
| AU2001244677A1 (en) | 2000-04-07 | 2001-10-23 | Eiken Kagaku Kabushiki Kaisha | Method of amplifying nucleic acid by using double-stranded nucleic acid as template |
| EP1327679B1 (en) | 2000-09-19 | 2009-07-22 | Eiken Kagaku Kabushiki Kaisha | Method of synthesizing polynucleotide |
| GB201506509D0 (en) | 2015-04-16 | 2015-06-03 | Univ Wageningen | Nuclease-mediated genome editing |
| EP3666895A1 (en) | 2015-06-18 | 2020-06-17 | The Broad Institute, Inc. | Novel crispr enzymes and systems |
| AU2016279062A1 (en) | 2015-06-18 | 2019-03-28 | Omar O. Abudayyeh | Novel CRISPR enzymes and systems |
| US9790490B2 (en) | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
| EP3365441A1 (en) | 2015-10-22 | 2018-08-29 | The Broad Institute Inc. | Type vi-b crispr enzymes and systems |
| US20190233814A1 (en) | 2015-12-18 | 2019-08-01 | The Broad Institute, Inc. | Novel crispr enzymes and systems |
| US10337051B2 (en) | 2016-06-16 | 2019-07-02 | The Regents Of The University Of California | Methods and compositions for detecting a target RNA |
| PT3551753T (pt) | 2016-12-09 | 2022-09-02 | Harvard College | Diagnósticos baseados num sistema efetor de crispr |
| CN112501254A (zh) | 2017-07-14 | 2021-03-16 | 上海吐露港生物科技有限公司 | 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 |
| CN111154921A (zh) * | 2020-03-19 | 2020-05-15 | 青岛国际旅行卫生保健中心(青岛海关口岸门诊部) | 基于可视化lamp的新型冠状病毒检测试剂盒及其检测方法 |
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2021
- 2021-06-11 US US18/009,832 patent/US20240052436A1/en active Pending
- 2021-06-11 CA CA3186580A patent/CA3186580A1/en active Pending
- 2021-06-11 TW TW110121518A patent/TW202219272A/zh unknown
- 2021-06-11 WO PCT/US2021/036925 patent/WO2021252836A1/en active Application Filing
- 2021-06-11 AU AU2021287968A patent/AU2021287968A1/en active Pending
- 2021-06-11 EP EP21737883.5A patent/EP4165218A1/en active Pending
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| AU2021287968A1 (en) | 2023-02-09 |
| CA3186580A1 (en) | 2021-12-16 |
| EP4165218A1 (en) | 2023-04-19 |
| US20240052436A1 (en) | 2024-02-15 |
| WO2021252836A1 (en) | 2021-12-16 |
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