RU2020100221A - Реакция амплификации с разрывом и удлинением (near) разновидностей респираторно-синцитиального вируса - Google Patents
Реакция амплификации с разрывом и удлинением (near) разновидностей респираторно-синцитиального вируса Download PDFInfo
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- RU2020100221A RU2020100221A RU2020100221A RU2020100221A RU2020100221A RU 2020100221 A RU2020100221 A RU 2020100221A RU 2020100221 A RU2020100221 A RU 2020100221A RU 2020100221 A RU2020100221 A RU 2020100221A RU 2020100221 A RU2020100221 A RU 2020100221A
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- 230000003321 amplification Effects 0.000 title claims 9
- 238000003199 nucleic acid amplification method Methods 0.000 title claims 9
- 238000006243 chemical reaction Methods 0.000 title claims 3
- 241000700605 Viruses Species 0.000 title 1
- 230000000241 respiratory effect Effects 0.000 title 1
- 125000003729 nucleotide group Chemical group 0.000 claims 45
- 239000002773 nucleotide Substances 0.000 claims 36
- 239000000203 mixture Substances 0.000 claims 30
- 108090000790 Enzymes Proteins 0.000 claims 29
- 102000004190 Enzymes Human genes 0.000 claims 29
- 238000000034 method Methods 0.000 claims 24
- 241000725643 Respiratory syncytial virus Species 0.000 claims 22
- 230000000295 complement effect Effects 0.000 claims 16
- 150000007523 nucleic acids Chemical group 0.000 claims 16
- 238000011144 upstream manufacturing Methods 0.000 claims 16
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 12
- 108090001074 Nucleocapsid Proteins Proteins 0.000 claims 10
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims 10
- 239000002751 oligonucleotide probe Substances 0.000 claims 10
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 9
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 9
- 101150095629 NS2 gene Proteins 0.000 claims 9
- 108090000623 proteins and genes Proteins 0.000 claims 9
- 108091081021 Sense strand Proteins 0.000 claims 8
- 230000000692 anti-sense effect Effects 0.000 claims 8
- 239000012472 biological sample Substances 0.000 claims 8
- 230000006641 stabilisation Effects 0.000 claims 8
- 238000011105 stabilization Methods 0.000 claims 8
- 108020004707 nucleic acids Proteins 0.000 claims 4
- 102000039446 nucleic acids Human genes 0.000 claims 4
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 3
- 230000000903 blocking effect Effects 0.000 claims 3
- 238000012986 modification Methods 0.000 claims 3
- 230000004048 modification Effects 0.000 claims 3
- 125000006850 spacer group Chemical group 0.000 claims 3
- 241000675112 Geobacillus bogazici Species 0.000 claims 2
- 241000211181 Manta Species 0.000 claims 2
- 108091034117 Oligonucleotide Proteins 0.000 claims 2
- 230000002255 enzymatic effect Effects 0.000 claims 2
- 238000006911 enzymatic reaction Methods 0.000 claims 2
- 229940125532 enzyme inhibitor Drugs 0.000 claims 2
- 239000002532 enzyme inhibitor Substances 0.000 claims 2
- 239000012634 fragment Substances 0.000 claims 2
- NFGXHKASABOEEW-LDRANXPESA-N methoprene Chemical compound COC(C)(C)CCCC(C)C\C=C\C(\C)=C\C(=O)OC(C)C NFGXHKASABOEEW-LDRANXPESA-N 0.000 claims 2
- 230000002285 radioactive effect Effects 0.000 claims 2
- 239000000523 sample Substances 0.000 claims 2
- 206010036790 Productive cough Diseases 0.000 claims 1
- 239000008280 blood Substances 0.000 claims 1
- 210000004369 blood Anatomy 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 210000003608 fece Anatomy 0.000 claims 1
- 210000004915 pus Anatomy 0.000 claims 1
- 210000003296 saliva Anatomy 0.000 claims 1
- 210000003802 sputum Anatomy 0.000 claims 1
- 208000024794 sputum Diseases 0.000 claims 1
- 210000002700 urine Anatomy 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/11—Antisense
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/507—Recombinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762552546P | 2017-08-31 | 2017-08-31 | |
| US62/552,546 | 2017-08-31 | ||
| PCT/US2018/048903 WO2019046610A1 (en) | 2017-08-31 | 2018-08-30 | REACTION OF SYNCHRONIZATION AMPLIFICATION AND (NEAR) EXTENSION OF SYNCYTIAL RESPIRATORY VIRUS SPECIES |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2020100221A true RU2020100221A (ru) | 2021-09-30 |
| RU2020100221A3 RU2020100221A3 (enExample) | 2022-03-11 |
Family
ID=65526105
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2020100221A RU2020100221A (ru) | 2017-08-31 | 2018-08-30 | Реакция амплификации с разрывом и удлинением (near) разновидностей респираторно-синцитиального вируса |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US11634770B2 (enExample) |
| EP (1) | EP3676394A4 (enExample) |
| JP (1) | JP2020533974A (enExample) |
| KR (1) | KR20200083444A (enExample) |
| CN (1) | CN111406118B (enExample) |
| AU (1) | AU2018326627C1 (enExample) |
| BR (1) | BR112020004071A2 (enExample) |
| CA (1) | CA3073954A1 (enExample) |
| RU (1) | RU2020100221A (enExample) |
| WO (1) | WO2019046610A1 (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9689031B2 (en) * | 2007-07-14 | 2017-06-27 | Ionian Technologies, Inc. | Nicking and extension amplification reaction for the exponential amplification of nucleic acids |
| GB201611469D0 (en) | 2016-06-30 | 2016-08-17 | Lumiradx Tech Ltd | Improvements in or relating to nucleic acid amplification processes |
| GB2569965A (en) | 2018-01-04 | 2019-07-10 | Lumiradx Uk Ltd | Improvements in or relating to amplification of nucleic acids |
| WO2025030178A1 (en) * | 2023-08-03 | 2025-02-06 | Abbott Laboratories | Isothermal amplification of nucleic acids |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060287267A1 (en) | 2001-05-18 | 2006-12-21 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of respiratory syncytial virus (RSV) expression using short interfering nucleic acid (siNA) |
| WO2004022701A2 (en) * | 2001-07-15 | 2004-03-18 | Keck Graduate Institute | Exponential amplification of nucleic acids using nicking agents |
| US6881835B2 (en) * | 2002-01-04 | 2005-04-19 | Dr. Chip Biotechnology Inc. | Detection of respiratory viruses |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| AU2003215391B2 (en) | 2002-02-21 | 2007-05-24 | Abbott Diagnostics Scarborough, Inc. | Recombinase Polymerase Amplification |
| CA2616241C (en) * | 2005-07-25 | 2012-02-07 | Asm Scientific, Inc. | Methods for multiplexing recombinase polymerase amplification |
| CA2650993C (en) | 2006-05-04 | 2015-06-16 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| US9689031B2 (en) * | 2007-07-14 | 2017-06-27 | Ionian Technologies, Inc. | Nicking and extension amplification reaction for the exponential amplification of nucleic acids |
| EP2438196B1 (en) | 2009-06-05 | 2016-12-21 | Alere San Diego, Inc. | Recombinase polymerase amplification reagents and kits |
| BR112014025262A8 (pt) * | 2012-04-09 | 2021-06-29 | Envirologix Inc | métodos para quantificar um produto específico em uma reação de amplificação de extensão e corte, para detectar uma pluralidade de produtos de reação distintos produzidos ao longo de uma única reação, para monitorar em tempo real uma reação de amplificação de extensão e corte, para monitorar em tempo real uma molécula-alvo de ácido nucleico em uma reação de amplificação de extensão e corte, para monitorar em tempo real uma molécula-alvo de ácido nucleico em uma amostra de teste, e para amplificar um polinucleotídeo, kit, e, oligonucleotídeo isolado |
| US10329601B2 (en) * | 2015-12-28 | 2019-06-25 | Ionian Technologies, Inc. | Nicking and extension amplification reaction (NEAR) of Streptococcus species |
-
2018
- 2018-08-30 RU RU2020100221A patent/RU2020100221A/ru unknown
- 2018-08-30 EP EP18849601.2A patent/EP3676394A4/en active Pending
- 2018-08-30 WO PCT/US2018/048903 patent/WO2019046610A1/en not_active Ceased
- 2018-08-30 CA CA3073954A patent/CA3073954A1/en active Pending
- 2018-08-30 AU AU2018326627A patent/AU2018326627C1/en active Active
- 2018-08-30 CN CN201880055127.XA patent/CN111406118B/zh active Active
- 2018-08-30 JP JP2020512607A patent/JP2020533974A/ja active Pending
- 2018-08-30 BR BR112020004071-1A patent/BR112020004071A2/pt not_active Application Discontinuation
- 2018-08-30 US US16/118,081 patent/US11634770B2/en active Active
- 2018-08-30 KR KR1020207009252A patent/KR20200083444A/ko not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| EP3676394A4 (en) | 2021-06-02 |
| CA3073954A1 (en) | 2019-03-07 |
| WO2019046610A1 (en) | 2019-03-07 |
| US20190194747A1 (en) | 2019-06-27 |
| KR20200083444A (ko) | 2020-07-08 |
| AU2018326627A1 (en) | 2020-01-30 |
| JP2020533974A (ja) | 2020-11-26 |
| BR112020004071A2 (pt) | 2020-09-24 |
| CN111406118A (zh) | 2020-07-10 |
| AU2018326627C1 (en) | 2024-12-12 |
| US11634770B2 (en) | 2023-04-25 |
| EP3676394A1 (en) | 2020-07-08 |
| AU2018326627B2 (en) | 2024-08-22 |
| CN111406118B (zh) | 2024-07-12 |
| RU2020100221A3 (enExample) | 2022-03-11 |
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