JP2011500092A5 - - Google Patents

Info

Publication number

JP2011500092A5

JP2011500092A5 JP2010531293A JP2010531293A JP2011500092A5 JP 2011500092 A5 JP2011500092 A5 JP 2011500092A5 JP 2010531293 A JP2010531293 A JP 2010531293A JP 2010531293 A JP2010531293 A JP 2010531293A JP 2011500092 A5 JP2011500092 A5 JP 2011500092A5

Authority

JP

Japan

Prior art keywords

population

hybridizing

oligonucleotides

oligonucleotide

nucleic acid

Prior art date

2008-10-24

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Pending

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• 108091034117 Oligonucleotide Proteins 0.000 claims description 100
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims description 67
• 150000007523 nucleic acids Chemical class 0.000 claims description 49
• 238000000034 method Methods 0.000 claims description 47
• 108020004707 nucleic acids Proteins 0.000 claims description 47
• 102000039446 nucleic acids Human genes 0.000 claims description 47
• 239000013615 primer Substances 0.000 claims description 36
• 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 30
• 239000002773 nucleotide Substances 0.000 claims description 28
• 125000003729 nucleotide group Chemical group 0.000 claims description 24
• 239000003155 DNA primer Substances 0.000 claims description 21
• 239000002299 complementary DNA Substances 0.000 claims description 14
• 108020004418 ribosomal RNA Proteins 0.000 claims description 13
• 239000003153 chemical reaction reagent Substances 0.000 claims description 10
• 230000002194 synthesizing effect Effects 0.000 claims description 8
• 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 7
• 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 7
• 102100034343 Integrase Human genes 0.000 claims description 7
• 238000012408 PCR amplification Methods 0.000 claims description 7
• 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims description 6
• 238000010804 cDNA synthesis Methods 0.000 claims description 6
• 230000003321 amplification Effects 0.000 claims description 5
• 238000003199 nucleic acid amplification method Methods 0.000 claims description 5
• 238000012163 sequencing technique Methods 0.000 claims description 5
• 230000002438 mitochondrial effect Effects 0.000 claims description 4
• 125000006850 spacer group Chemical group 0.000 claims description 4
• 230000000295 complement effect Effects 0.000 claims description 3
• 230000037452 priming Effects 0.000 claims description 3
• 238000013518 transcription Methods 0.000 claims 1
• 230000035897 transcription Effects 0.000 claims 1
• 108020004414 DNA Proteins 0.000 description 11
• 238000000137 annealing Methods 0.000 description 3
• 102000053602 DNA Human genes 0.000 description 2
• 108091028043 Nucleic acid sequence Proteins 0.000 description 2
• 238000002372 labelling Methods 0.000 description 2
• 210000004962 mammalian cell Anatomy 0.000 description 2
• 230000008488 polyadenylation Effects 0.000 description 2
• 102000012410 DNA Ligases Human genes 0.000 description 1
• 108010061982 DNA Ligases Proteins 0.000 description 1
• 229910019142 PO4 Inorganic materials 0.000 description 1
• 229940122208 Ribonuclease inhibitor Drugs 0.000 description 1
• 102100037968 Ribonuclease inhibitor Human genes 0.000 description 1
• 101710141795 Ribonuclease inhibitor Proteins 0.000 description 1
• 239000007983 Tris buffer Substances 0.000 description 1
• 150000003863 ammonium salts Chemical class 0.000 description 1
• 210000004027 cell Anatomy 0.000 description 1
• 239000003638 chemical reducing agent Substances 0.000 description 1
• 238000010367 cloning Methods 0.000 description 1
• 239000013599 cloning vector Substances 0.000 description 1
• 159000000003 magnesium salts Chemical class 0.000 description 1
• NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
• 239000010452 phosphate Substances 0.000 description 1
• XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 description 1
• 239000003161 ribonuclease inhibitor Substances 0.000 description 1
• 239000000758 substrate Substances 0.000 description 1
• 230000002103 transcriptional effect Effects 0.000 description 1
• LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
• 239000013598 vector Substances 0.000 description 1