JP2014014371A5 - - Google Patents
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- JP2014014371A5 JP2014014371A5 JP2013185104A JP2013185104A JP2014014371A5 JP 2014014371 A5 JP2014014371 A5 JP 2014014371A5 JP 2013185104 A JP2013185104 A JP 2013185104A JP 2013185104 A JP2013185104 A JP 2013185104A JP 2014014371 A5 JP2014014371 A5 JP 2014014371A5
- Authority
- JP
- Japan
- Prior art keywords
- primer
- oligonucleotide
- rnase
- nucleic acid
- cleavage domain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000013615 primer Substances 0.000 claims 130
- 108091034117 Oligonucleotide Proteins 0.000 claims 108
- 238000003776 cleavage reaction Methods 0.000 claims 85
- 230000007017 scission Effects 0.000 claims 84
- 238000000034 method Methods 0.000 claims 79
- 230000000903 blocking effect Effects 0.000 claims 61
- 150000007523 nucleic acids Chemical class 0.000 claims 58
- 108010083644 Ribonucleases Proteins 0.000 claims 56
- 102000006382 Ribonucleases Human genes 0.000 claims 56
- 108020004707 nucleic acids Proteins 0.000 claims 44
- 102000039446 nucleic acids Human genes 0.000 claims 44
- 102000004190 Enzymes Human genes 0.000 claims 35
- 108090000790 Enzymes Proteins 0.000 claims 35
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 34
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 27
- 239000011541 reaction mixture Substances 0.000 claims 27
- 239000000523 sample Substances 0.000 claims 24
- 230000000295 complement effect Effects 0.000 claims 20
- 125000003729 nucleotide group Chemical group 0.000 claims 16
- 239000000758 substrate Substances 0.000 claims 13
- 102100034343 Integrase Human genes 0.000 claims 12
- 230000004543 DNA replication Effects 0.000 claims 11
- 239000002773 nucleotide Substances 0.000 claims 11
- 229920000642 polymer Polymers 0.000 claims 10
- 101710203526 Integrase Proteins 0.000 claims 9
- 150000001875 compounds Chemical class 0.000 claims 9
- 238000012986 modification Methods 0.000 claims 9
- 230000004048 modification Effects 0.000 claims 9
- 238000006243 chemical reaction Methods 0.000 claims 8
- 229920002477 rna polymer Polymers 0.000 claims 8
- 125000006850 spacer group Chemical group 0.000 claims 8
- 102000003960 Ligases Human genes 0.000 claims 7
- 108090000364 Ligases Proteins 0.000 claims 7
- 125000000217 alkyl group Chemical group 0.000 claims 7
- -1 polymerase Proteins 0.000 claims 7
- 125000000446 sulfanediyl group Chemical group *S* 0.000 claims 7
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 6
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims 6
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 claims 6
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims 5
- 230000000694 effects Effects 0.000 claims 5
- 238000009396 hybridization Methods 0.000 claims 5
- 239000002751 oligonucleotide probe Substances 0.000 claims 5
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 claims 4
- 239000005977 Ethylene Substances 0.000 claims 4
- 150000001412 amines Chemical class 0.000 claims 4
- 230000003321 amplification Effects 0.000 claims 4
- 125000001369 canonical nucleoside group Chemical group 0.000 claims 4
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims 4
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims 4
- 125000000325 methylidene group Chemical group [H]C([H])=* 0.000 claims 4
- 230000035772 mutation Effects 0.000 claims 4
- 238000003199 nucleic acid amplification method Methods 0.000 claims 4
- UEZVMMHDMIWARA-UHFFFAOYSA-M phosphonate Chemical compound [O-]P(=O)=O UEZVMMHDMIWARA-UHFFFAOYSA-M 0.000 claims 4
- 239000001226 triphosphate Substances 0.000 claims 4
- 235000011178 triphosphate Nutrition 0.000 claims 4
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims 4
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims 3
- 229930024421 Adenine Natural products 0.000 claims 3
- 108020004414 DNA Proteins 0.000 claims 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 3
- 229960000643 adenine Drugs 0.000 claims 3
- 229940104302 cytosine Drugs 0.000 claims 3
- 239000007850 fluorescent dye Substances 0.000 claims 3
- 125000001153 fluoro group Chemical group F* 0.000 claims 3
- 239000000203 mixture Substances 0.000 claims 3
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 3
- 239000002987 primer (paints) Substances 0.000 claims 3
- 238000006467 substitution reaction Methods 0.000 claims 3
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical class CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 claims 3
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 claims 2
- 241001148023 Pyrococcus abyssi Species 0.000 claims 2
- 241000205156 Pyrococcus furiosus Species 0.000 claims 2
- 108091028664 Ribonucleotide Proteins 0.000 claims 2
- 241000205091 Sulfolobus solfataricus Species 0.000 claims 2
- 241001235254 Thermococcus kodakarensis Species 0.000 claims 2
- 238000003556 assay Methods 0.000 claims 2
- 150000001768 cations Chemical class 0.000 claims 2
- 230000001351 cycling effect Effects 0.000 claims 2
- 239000005546 dideoxynucleotide Substances 0.000 claims 2
- 239000012634 fragment Substances 0.000 claims 2
- 230000001404 mediated effect Effects 0.000 claims 2
- 239000002777 nucleoside Substances 0.000 claims 2
- 239000002336 ribonucleotide Substances 0.000 claims 2
- 125000002652 ribonucleotide group Chemical group 0.000 claims 2
- 238000012163 sequencing technique Methods 0.000 claims 2
- 230000002194 synthesizing effect Effects 0.000 claims 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
- 108700028369 Alleles Proteins 0.000 claims 1
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 claims 1
- 239000003155 DNA primer Substances 0.000 claims 1
- 102100029764 DNA-directed DNA/RNA polymerase mu Human genes 0.000 claims 1
- 241000194036 Lactococcus Species 0.000 claims 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims 1
- 241000203407 Methanocaldococcus jannaschii Species 0.000 claims 1
- 229910019142 PO4 Inorganic materials 0.000 claims 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims 1
- ZADPBFCGQRWHPN-UHFFFAOYSA-N boronic acid Chemical compound OBO ZADPBFCGQRWHPN-UHFFFAOYSA-N 0.000 claims 1
- 229910017052 cobalt Inorganic materials 0.000 claims 1
- 239000010941 cobalt Substances 0.000 claims 1
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims 1
- 239000005547 deoxyribonucleotide Substances 0.000 claims 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 claims 1
- 150000005690 diesters Chemical class 0.000 claims 1
- NAGJZTKCGNOGPW-UHFFFAOYSA-K dioxido-sulfanylidene-sulfido-$l^{5}-phosphane Chemical compound [O-]P([O-])([S-])=S NAGJZTKCGNOGPW-UHFFFAOYSA-K 0.000 claims 1
- 239000000975 dye Substances 0.000 claims 1
- 230000007062 hydrolysis Effects 0.000 claims 1
- 238000006460 hydrolysis reaction Methods 0.000 claims 1
- 229910052749 magnesium Inorganic materials 0.000 claims 1
- 239000011777 magnesium Substances 0.000 claims 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 claims 1
- NBTOZLQBSIZIKS-UHFFFAOYSA-N methoxide Chemical compound [O-]C NBTOZLQBSIZIKS-UHFFFAOYSA-N 0.000 claims 1
- 239000000178 monomer Substances 0.000 claims 1
- 229910052759 nickel Inorganic materials 0.000 claims 1
- 125000003835 nucleoside group Chemical group 0.000 claims 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims 1
- 239000010452 phosphate Substances 0.000 claims 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 claims 1
- 230000003362 replicative effect Effects 0.000 claims 1
- 125000001981 tert-butyldimethylsilyl group Chemical group [H]C([H])([H])[Si]([H])(C([H])([H])[H])[*]C(C([H])([H])[H])(C([H])([H])[H])C([H])([H])[H] 0.000 claims 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 claims 1
- 125000000025 triisopropylsilyl group Chemical group C(C)(C)[Si](C(C)C)(C(C)C)* 0.000 claims 1
- 238000011144 upstream manufacturing Methods 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
- 239000011701 zinc Substances 0.000 claims 1
- 229910052725 zinc Inorganic materials 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US4920408P | 2008-04-30 | 2008-04-30 | |
| US61/049,204 | 2008-04-30 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011507669A Division JP5539325B2 (ja) | 2008-04-30 | 2009-04-30 | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015116458A Division JP2015221036A (ja) | 2008-04-30 | 2015-06-09 | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2014014371A JP2014014371A (ja) | 2014-01-30 |
| JP2014014371A5 true JP2014014371A5 (enExample) | 2014-06-26 |
Family
ID=41203821
Family Applications (6)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011507669A Active JP5539325B2 (ja) | 2008-04-30 | 2009-04-30 | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
| JP2013185104A Pending JP2014014371A (ja) | 2008-04-30 | 2013-09-06 | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
| JP2015116458A Ceased JP2015221036A (ja) | 2008-04-30 | 2015-06-09 | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
| JP2016246235A Active JP6453296B2 (ja) | 2008-04-30 | 2016-12-20 | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
| JP2016246236A Expired - Fee Related JP6276835B2 (ja) | 2008-04-30 | 2016-12-20 | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
| JP2018038239A Active JP6510695B2 (ja) | 2008-04-30 | 2018-03-05 | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011507669A Active JP5539325B2 (ja) | 2008-04-30 | 2009-04-30 | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
Family Applications After (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015116458A Ceased JP2015221036A (ja) | 2008-04-30 | 2015-06-09 | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
| JP2016246235A Active JP6453296B2 (ja) | 2008-04-30 | 2016-12-20 | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
| JP2016246236A Expired - Fee Related JP6276835B2 (ja) | 2008-04-30 | 2016-12-20 | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
| JP2018038239A Active JP6510695B2 (ja) | 2008-04-30 | 2018-03-05 | 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ |
Country Status (7)
| Country | Link |
|---|---|
| US (4) | US20090325169A1 (enExample) |
| EP (6) | EP2644707B1 (enExample) |
| JP (6) | JP5539325B2 (enExample) |
| AU (1) | AU2009242546B2 (enExample) |
| CA (1) | CA2722541C (enExample) |
| DK (2) | DK2644707T3 (enExample) |
| WO (1) | WO2009135093A2 (enExample) |
Families Citing this family (77)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10227641B2 (en) | 2008-04-30 | 2019-03-12 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| EP2644707B1 (en) | 2008-04-30 | 2015-06-03 | Integrated Dna Technologies, Inc. | RNase-H-based assays utilizing modified RNA monomers |
| WO2014143228A1 (en) * | 2013-03-15 | 2014-09-18 | Integrated Dna Technologies, Inc. | Rnase h-based assays utilizing modified rna monomers |
| EP3249053A1 (en) | 2009-03-27 | 2017-11-29 | Life Technologies Corporation | Methods, compositions, and kits for detecting allelic variants |
| JP2013507910A (ja) * | 2009-10-13 | 2013-03-07 | シンテッツァ モレキュラー ディテクション イスラエル リミテッド | 核酸試料から標的配列を増幅するための方法および組成物 |
| WO2011060014A1 (en) | 2009-11-13 | 2011-05-19 | Integrated Dna Technologies, Inc. | Small rna detection assays |
| JP2011188855A (ja) * | 2010-03-11 | 2011-09-29 | Samsung Techwin Co Ltd | 切断可能なキメラプローブを使用したサルモネラのリアルタイム検出方法 |
| US8618253B2 (en) * | 2010-05-25 | 2013-12-31 | Samsung Techwin Co., Ltd. | Modified RNAse H and detection of nucleic acid amplification |
| US9850527B2 (en) | 2010-06-14 | 2017-12-26 | National University Of Singapore | Modified stem-loop oligonucleotide mediated reverse transcription and base-spacing constrained quantitative PCR |
| CA2810856C (en) | 2010-08-13 | 2022-05-17 | Envirologix Inc. | Compositions and methods for quantifying a nucleic acid sequence in a sample |
| US20120052482A1 (en) * | 2010-08-27 | 2012-03-01 | Samsung Techwin Co., Ltd. | Kit for detecting hepatitis c virus and method of detecting hepatitis c virus using the same |
| US20120052495A1 (en) * | 2010-08-30 | 2012-03-01 | Samsung Techwin Co., Ltd. | Oligonucleotides for detecting listeria monocytogenes and use thereof |
| US20120052501A1 (en) * | 2010-08-30 | 2012-03-01 | Samsung Techwin Co., Ltd. | Kit for detecting htlv strains and use thereof |
| US20120052492A1 (en) * | 2010-08-30 | 2012-03-01 | Samsung Techwin Co., Ltd. | Oligonucleotides for detecting listeria spp. and use thereof |
| US20120052500A1 (en) * | 2010-08-30 | 2012-03-01 | Samsung Techwin Co., Ltd. | Kit for detecting chlamydia trachomatis strains and method for detecting chlamydia trachomatis strains using the same |
| US20120052503A1 (en) * | 2010-08-30 | 2012-03-01 | Samsung Techwin Co., Ltd. | Kit for detecting neisseria gonorrhoeae strains and method for detecting neisseria gonorrhoeae strains using the same |
| US20120088246A1 (en) * | 2010-10-07 | 2012-04-12 | Samsung Techwin Co., Ltd. | Real time pcr detection of single nucleotide polymorphisms |
| US9074251B2 (en) | 2011-02-10 | 2015-07-07 | Illumina, Inc. | Linking sequence reads using paired code tags |
| EP2663654A1 (en) * | 2011-01-14 | 2013-11-20 | Life Technologies Corporation | Methods, compositions, and kits for detecting rare cells |
| WO2012106546A2 (en) | 2011-02-02 | 2012-08-09 | University Of Washington Through Its Center For Commercialization | Massively parallel continguity mapping |
| WO2012135053A2 (en) * | 2011-03-25 | 2012-10-04 | Integrated Dna Technologies, Inc. | Rnase h-based assays utilizing modified rna monomers |
| JP2014518071A (ja) * | 2011-06-15 | 2014-07-28 | エヌエスイー プロダクツ インコーポレイテッド | カロリー制限およびカロリー制限模倣物の同定用マーカー |
| US9603949B2 (en) | 2011-09-01 | 2017-03-28 | University Of Iowa Research Foundation | Oligonucleotide-based probes for detection of bacterial nucleases |
| WO2013123238A1 (en) | 2012-02-14 | 2013-08-22 | Great Basin Scientific | Methods of isothermal amplification using blocked primers |
| BR112014025262A8 (pt) | 2012-04-09 | 2021-06-29 | Envirologix Inc | métodos para quantificar um produto específico em uma reação de amplificação de extensão e corte, para detectar uma pluralidade de produtos de reação distintos produzidos ao longo de uma única reação, para monitorar em tempo real uma reação de amplificação de extensão e corte, para monitorar em tempo real uma molécula-alvo de ácido nucleico em uma reação de amplificação de extensão e corte, para monitorar em tempo real uma molécula-alvo de ácido nucleico em uma amostra de teste, e para amplificar um polinucleotídeo, kit, e, oligonucleotídeo isolado |
| US20130310269A1 (en) * | 2012-05-04 | 2013-11-21 | Austin So | Hot-start digital pcr |
| DK2855692T3 (da) | 2012-05-24 | 2019-10-14 | Univ Utah Res Found | Ekstrem pcr |
| WO2014018093A1 (en) * | 2012-07-26 | 2014-01-30 | Illumina, Inc. | Compositions and methods for the amplification of nucleic acids |
| US9683230B2 (en) | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
| AU2014205110A1 (en) | 2013-01-13 | 2015-08-27 | Unitaq Bio | Methods and compositions for PCR using blocked and universal primers |
| WO2014134144A1 (en) * | 2013-02-28 | 2014-09-04 | The General Hospital Corporation | Mirna profiling compositions and methods of use |
| EP2970951B1 (en) | 2013-03-13 | 2019-02-20 | Illumina, Inc. | Methods for nucleic acid sequencing |
| US10119134B2 (en) | 2013-03-15 | 2018-11-06 | Abvitro Llc | Single cell bar-coding for antibody discovery |
| EP2971160B1 (en) | 2013-03-15 | 2018-05-30 | Bio-Rad Laboratories, Inc. | Digital assays for mutation detection |
| CA2930595C (en) * | 2013-11-14 | 2023-09-19 | Integrated Dna Technologies, Inc. | Dna polymerase mutants having enhanced template discrimination activity |
| US10385388B2 (en) | 2013-12-06 | 2019-08-20 | Swift Biosciences, Inc. | Cleavable competitor polynucleotides |
| AU2014364926B2 (en) | 2013-12-20 | 2017-12-14 | Illumina, Inc. | Preserving genomic connectivity information in fragmented genomic DNA samples |
| CA2939124C (en) * | 2014-02-07 | 2023-08-29 | University Of Iowa Research Foundation | Oligonucleotide-based probes and methods for detection of microbes |
| GB201403216D0 (en) * | 2014-02-24 | 2014-04-09 | Cambridge Epigenetix Ltd | Nucleic acid sample preparation |
| WO2015164494A1 (en) | 2014-04-22 | 2015-10-29 | Envirologix, Inc. | Compositions and methods for enhancing and/or predicting dna amplification |
| KR102465287B1 (ko) * | 2014-08-11 | 2022-11-09 | 루미넥스 코포레이션 | 핵산 검정에서 개선된 용융 판별 및 멀티플렉싱을 위한 프로브 |
| US20160115473A1 (en) * | 2014-08-14 | 2016-04-28 | Abbott Molecular Inc. | Multifunctional oligonucleotides |
| SG10201911069WA (en) | 2014-09-15 | 2020-01-30 | Abvitro Llc | High-throughput nucleotide library sequencing |
| KR102472027B1 (ko) | 2014-10-17 | 2022-11-30 | 일루미나 케임브리지 리미티드 | 근접 보존 전위 |
| US10793922B2 (en) | 2014-10-20 | 2020-10-06 | Envirologix Inc. | Compositions and methods for detecting an RNA virus |
| WO2016081551A1 (en) | 2014-11-20 | 2016-05-26 | Ampliwise Inc. | Compositions and methods for nucleic acid amplification |
| US10619199B2 (en) * | 2015-03-06 | 2020-04-14 | Sigma-Aldrich Co. Llc | Nucleic acid amplification and library preparation |
| AU2016313095A1 (en) * | 2015-08-24 | 2017-11-23 | Qiagen Gmbh | Method for generating a RNA-sequencing library |
| WO2018057051A1 (en) | 2016-09-24 | 2018-03-29 | Abvitro Llc | Affinity-oligonucleotide conjugates and uses thereof |
| WO2017053903A1 (en) | 2015-09-24 | 2017-03-30 | Abvitro Llc | Single amplicon activated exclusion pcr |
| MY193806A (en) | 2015-09-24 | 2022-10-27 | Abvitro Llc | Affinity-oligonucleotide conjugates and uses thereof |
| AU2016326734B2 (en) | 2015-09-25 | 2022-07-07 | Abvitro Llc | High throughput process for T cell receptor target identification of natively-paired T cell receptor sequences |
| ES2916153T3 (es) * | 2015-11-05 | 2022-06-28 | Univ Utah Res Found | PCR extrema para transcripción inversa |
| US20210395799A1 (en) * | 2015-11-25 | 2021-12-23 | Integrated Dna Technologies, Inc. | Methods for variant detection |
| CN116064748A (zh) * | 2015-11-25 | 2023-05-05 | 综合基因技术公司 | 用于变体检测的方法 |
| WO2017136387A1 (en) * | 2016-02-01 | 2017-08-10 | Integrated Dna Technologies, Inc. | Cleavable primers for isothermal amplification |
| CN107083427B (zh) * | 2016-04-01 | 2021-07-30 | 广州市基准医疗有限责任公司 | Dna连接酶介导的dna扩增技术 |
| US11268117B2 (en) | 2016-06-10 | 2022-03-08 | Life Technologies Corporation | Methods and compositions for nucleic acid amplification |
| EP3610030B1 (en) | 2017-04-13 | 2024-08-07 | Integrated DNA Technologies, Inc. | Methods for variant detection |
| CN111148849A (zh) | 2017-05-26 | 2020-05-12 | 阿布维托有限责任公司 | 高通量多核苷酸文库测序和转录组分析 |
| US10081829B1 (en) | 2017-06-13 | 2018-09-25 | Genetics Research, Llc | Detection of targeted sequence regions |
| WO2018231985A1 (en) * | 2017-06-13 | 2018-12-20 | Genetics Research, Llc, D/B/A Zs Genetics, Inc. | Polynucleic acid molecule enrichment methodologies |
| US10527608B2 (en) | 2017-06-13 | 2020-01-07 | Genetics Research, Llc | Methods for rare event detection |
| US20200292526A1 (en) | 2017-09-07 | 2020-09-17 | Juno Therapeutics, Inc. | Methods of identifying cellular attributes related to outcomes associated with cell therapy |
| JP7421202B2 (ja) | 2017-09-29 | 2024-01-24 | ユニバーシティー オブ アイオワ リサーチ ファンデーション | デジタルヌクレアーゼ検出組成物および方法 |
| CN112513265A (zh) * | 2018-05-22 | 2021-03-16 | 博诚研究中心 | 用于人类癌症检测的修饰核酸的靶向富集和测序 |
| GB201812192D0 (en) | 2018-07-26 | 2018-09-12 | Ttp Plc | Variable temperature reactor, heater and control circuit for the same |
| CN109913538A (zh) * | 2018-09-06 | 2019-06-21 | 湖南融健基因生物科技有限公司 | 一种快速检测snp位点的荧光定量试剂盒 |
| US11591629B2 (en) * | 2019-12-23 | 2023-02-28 | Cheng-Yao Chen | Method and kit for template-independent nucleic acid synthesis |
| KR20210109745A (ko) * | 2020-02-28 | 2021-09-07 | 주식회사 누리바이오 | 단일 표적 유전자의 유전적 변이 실시간 검출용 단일핵산 및 이를 이용한 검출 방법 |
| WO2021259201A1 (zh) * | 2020-06-22 | 2021-12-30 | 苏州新海生物科技股份有限公司 | 一种核酸配体及其应用 |
| CN113897420A (zh) * | 2020-06-22 | 2022-01-07 | 上海思路迪生物医学科技有限公司 | 基于扩增子测序的大片段重排检测的引物组合物、试剂盒和检测方法 |
| CN112063689A (zh) * | 2020-08-21 | 2020-12-11 | 上海思路迪生物医学科技有限公司 | 一种高效rna反转录方法及rna和dna同步建库方法 |
| CN113981069B (zh) * | 2021-11-10 | 2023-08-15 | 郑州华沃生物科技有限公司 | 检测adrb1基因g1165c多态性的引物、试剂盒及其检测方法和应用 |
| CN115948388A (zh) * | 2022-12-30 | 2023-04-11 | 纳昂达(南京)生物科技有限公司 | 特异性捕获引物、靶向捕获探针组合物、靶向捕获文库的构建方法及应用 |
| WO2025096569A1 (en) * | 2023-10-30 | 2025-05-08 | The Penn State Research Foundation | Methods and compositions for real-time antimicrobial resistance profiling |
| WO2025207561A1 (en) * | 2024-03-27 | 2025-10-02 | Cepheid | Isothermal amplification using novel synthetic oligonucleotides |
Family Cites Families (85)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| CA1340522C (en) | 1987-03-10 | 1999-05-04 | Heinz Dobeli | Fusion proteins containing neighbouring histidines for improved purification |
| US6031091A (en) | 1987-09-21 | 2000-02-29 | Gen-Probe Incorporated | Non-nucleotide linking reagents for nucleotide probes |
| US5403711A (en) | 1987-11-30 | 1995-04-04 | University Of Iowa Research Foundation | Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved |
| IE61148B1 (en) | 1988-03-10 | 1994-10-05 | Ici Plc | Method of detecting nucleotide sequences |
| US5002867A (en) | 1988-04-25 | 1991-03-26 | Macevicz Stephen C | Nucleic acid sequence determination by multiple mixed oligonucleotide probes |
| JP2955759B2 (ja) | 1988-07-20 | 1999-10-04 | セゲブ・ダイアグノスティックス・インコーポレイテッド | 核酸配列を増幅及び検出する方法 |
| US5137806A (en) | 1989-12-11 | 1992-08-11 | Board Of Regents, The University Of Texas System | Methods and compositions for the detection of sequences in selected DNA molecules |
| US5516663A (en) * | 1990-01-26 | 1996-05-14 | Abbott Laboratories | Ligase chain reaction with endonuclease IV correction and contamination control |
| ES2141088T3 (es) | 1990-02-16 | 2000-03-16 | Hoffmann La Roche | Mejoras en la especificidad y conveniencia de la reaccion en cadena de la polimerasa. |
| US5494810A (en) | 1990-05-03 | 1996-02-27 | Cornell Research Foundation, Inc. | Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| JPH0515439A (ja) | 1991-07-08 | 1993-01-26 | Matsushita Electric Ind Co Ltd | ジヤー炊飯器の制御回路 |
| EP0655090B1 (en) | 1992-04-27 | 2000-12-27 | The Trustees Of Dartmouth College | Detection of gene sequences in biological fluids |
| CA2135073C (en) | 1992-05-06 | 2002-11-19 | Daniel L. Kacian | Nucleic acid sequence amplification method, composition and kit |
| US5338671A (en) * | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
| ATE206764T1 (de) * | 1994-07-16 | 2001-10-15 | Roche Diagnostics Gmbh | Verfahren zum sensitiven nachweis von nukleinsäuren |
| FR2724934B1 (fr) * | 1994-09-26 | 1997-01-24 | Bio Merieux | Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique |
| WO1996015271A1 (en) * | 1994-11-16 | 1996-05-23 | Abbott Laboratories | Multiplex ligations-dependent amplification |
| US20030165908A1 (en) | 1994-12-30 | 2003-09-04 | Georgetown University | Fluorometric assay for detecting nucleic acid cleavage |
| WO1996021144A1 (en) | 1994-12-30 | 1996-07-11 | Georgetown University | Fluorometric assay for detecting nucleic acid cleavage |
| US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| US5898031A (en) | 1996-06-06 | 1999-04-27 | Isis Pharmaceuticals, Inc. | Oligoribonucleotides for cleaving RNA |
| EP0912767B1 (en) | 1996-07-16 | 2006-09-13 | Gen-Probe Incorporated | Methods for detecting and amplifying nucleic acid sequences using modified oligonucleotides having increased target specific tm |
| US5853990A (en) * | 1996-07-26 | 1998-12-29 | Edward E. Winger | Real time homogeneous nucleotide assay |
| EP0866071B1 (en) | 1997-03-20 | 2004-10-20 | F. Hoffmann-La Roche Ag | Modified primers |
| WO1998046793A1 (en) | 1997-04-16 | 1998-10-22 | Trevigen, Inc. | Detection and mapping of point mutations using partial digestion |
| US5846726A (en) | 1997-05-13 | 1998-12-08 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
| WO1999002728A1 (en) | 1997-07-11 | 1999-01-21 | Brax Group Limited | Characterising nucleic acids |
| JPH1132772A (ja) * | 1997-07-24 | 1999-02-09 | Mitsubishi Chem Corp | 耐熱性リボヌクレアーゼｈ及びそれをコードするｄｎａ |
| GB9725237D0 (en) | 1997-11-29 | 1998-01-28 | Secr Defence | Amplification system |
| WO2005021776A2 (en) * | 2003-08-21 | 2005-03-10 | Isis Pharmaceuticals, Inc. | METHODS OF USING MAMMALIAN RNase H AND COMPOSITIONS THEREOF |
| EP1036165A4 (en) | 1997-12-04 | 2003-05-14 | Isis Pharmaceuticals Inc | HUMAN RNASE-H AND COMPOSITIONS AND USES THEREOF |
| US6248877B1 (en) | 1998-04-07 | 2001-06-19 | Biolink Partners | Solid phase synthesis of organic compounds via phosphitylating reagents |
| AU3174600A (en) * | 1999-03-10 | 2000-09-28 | Asm Scientific, Inc. | A method for direct nucleic acid sequencing |
| IL148091A0 (en) | 1999-09-13 | 2002-09-12 | Nugen Technologies Inc | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
| US6274353B1 (en) * | 1999-09-22 | 2001-08-14 | Genecopoeia, Inc. | Method and compositions for improved polynucleotide synthesis |
| DE60027040T2 (de) | 1999-10-29 | 2006-11-23 | Stratagene California, La Jolla | Zusammensetzungen und methoden zur verwendung von dna polymerasen |
| WO2001061015A2 (en) | 2000-02-17 | 2001-08-23 | Qiagen Gmbh | Thermostable chimeric nucleic acid polymerases and uses thereof |
| US7033763B2 (en) | 2000-02-23 | 2006-04-25 | City Of Hope | Pyrophosphorolysis activated polymerization (PAP) |
| CA2395391C (en) | 2000-02-23 | 2011-08-02 | City Of Hope | Pyrophosphorolysis activated polymerization (pap): application to allele-specific amplification and nucleic acid sequence determination |
| US6596490B2 (en) | 2000-07-14 | 2003-07-22 | Applied Gene Technologies, Inc. | Nucleic acid hairpin probes and uses thereof |
| EP1318197B1 (en) | 2000-09-14 | 2008-10-15 | Takara Bio Inc. | Thermotolerant ribonuclease h |
| CA2430329A1 (en) | 2000-12-13 | 2002-06-20 | Nugen Technologies, Inc. | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
| CN100351393C (zh) | 2001-02-15 | 2007-11-28 | 宝生物工程株式会社 | 核苷酸多态性的检测方法 |
| US6596489B2 (en) | 2001-03-30 | 2003-07-22 | Applied Gene Technologies | Methods and compositions for analyzing nucleotide sequence mismatches using RNase H |
| EP1275735A1 (en) | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Composition and method for hot start nucleic acid amplification |
| EP1420069A4 (en) | 2001-08-20 | 2005-11-02 | Takara Bio Inc | nucleic acid amplification |
| CA2409775C (en) * | 2001-12-03 | 2010-07-13 | F. Hoffmann-La Roche Ag | Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro |
| AU2003213652A1 (en) * | 2002-03-01 | 2003-09-16 | Integrated Dna Technologies, Inc. | Polynomial amplification of nucleic acids |
| US7629152B2 (en) | 2002-03-01 | 2009-12-08 | Integrated Dna Technologies, Inc. | Methods for amplifying polymeric nucleic acids |
| SI3363809T1 (sl) * | 2002-08-23 | 2020-08-31 | Illumina Cambridge Limited | Modificirani nukleotidi za polinukleotidno sekvenciranje |
| JP4105693B2 (ja) * | 2002-08-30 | 2008-06-25 | タカラバイオ株式会社 | 耐熱性リボヌクレアーゼｈ |
| ATE411400T1 (de) | 2002-11-21 | 2008-10-15 | Epict Technologies | Verfahren zur verwendung von riboprimern zur strangverdrängungsreplikation von zielsequenzen |
| US20040175737A1 (en) * | 2002-12-23 | 2004-09-09 | Wyeth | Assay for RNase H activity |
| JP2005006587A (ja) * | 2003-06-20 | 2005-01-13 | Takara Bio Inc | 標的核酸の増幅及び／又は検出方法 |
| AU2004203649B2 (en) | 2003-08-12 | 2006-01-12 | F. Hoffmann-La Roche Ag | Thermostable Taq polymerase fragment |
| WO2005049849A2 (en) | 2003-11-14 | 2005-06-02 | Integrated Dna Technologies, Inc. | Fluorescence quenching azo dyes, their methods of preparation and use |
| KR101041106B1 (ko) | 2003-11-25 | 2011-06-13 | 한면기 | 핵산과 단백질의 신규한 실시간 탐지방법 |
| WO2007062495A1 (en) * | 2005-11-30 | 2007-06-07 | Roy Rabindranauth Sooknanan | Selective terminal tagging of nucleic acids |
| US7344834B2 (en) | 2003-12-08 | 2008-03-18 | Cytyc Corporation | Method for DNA amplification using DNA blocking probes |
| JP4249186B2 (ja) * | 2003-12-10 | 2009-04-02 | タカラバイオ株式会社 | 核酸の増幅方法 |
| WO2005123913A2 (en) * | 2004-06-09 | 2005-12-29 | Nustar Laboratory | Reversibly modified thermostable enzyme compositions and methods of making and using the same |
| US20060110765A1 (en) | 2004-11-23 | 2006-05-25 | Wang Xiao B | Detection of nucleic acid variation by cleavage-amplification (CleavAmp) method |
| WO2006070666A1 (ja) * | 2004-12-28 | 2006-07-06 | Takara Bio Inc. | 遺伝子多型の同時検出方法 |
| WO2006074233A2 (en) * | 2005-01-06 | 2006-07-13 | Applera Corporation | Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification |
| WO2006081426A2 (en) * | 2005-01-28 | 2006-08-03 | Applera Corporation | Compositions and methods for terminating a sequencing reaction at a specific location in a target dna template |
| EP1844162B1 (en) | 2005-02-01 | 2014-10-15 | Applied Biosystems, LLC | Method for identifying a sequence in a polynucleotide |
| US20060211024A1 (en) | 2005-03-10 | 2006-09-21 | Gwc Technologies Incorporated | Methods for analysis of a nucleic acid sample |
| US20090068643A1 (en) | 2005-11-23 | 2009-03-12 | Integrated Dna Technologies, Inc. | Dual Function Primers for Amplifying DNA and Methods of Use |
| WO2007103558A2 (en) | 2006-03-09 | 2007-09-13 | The Regents Of The University Of California | Hybrid energy transfer for nucleic acid detection |
| WO2007106907A2 (en) | 2006-03-15 | 2007-09-20 | Integrated Dna Technologies, Inc. | Nucleic acid monomers with 2'-chemical moieties |
| DE602007013223D1 (de) | 2006-06-01 | 2011-04-28 | Trilink Biotechnologies San Diego | Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation |
| GB0611444D0 (en) * | 2006-06-09 | 2006-07-19 | Medical Res Council Technology | Rnase H2 complex and genes therefor |
| AU2007260707A1 (en) * | 2006-06-16 | 2007-12-21 | Pacific Biosciences Of California, Inc. | Controlled initiation of primer extension |
| JP2008072427A (ja) | 2006-09-14 | 2008-03-27 | Konica Minolta Business Technologies Inc | 画像形成装置及びクライアント／サーバ型情報処理システム並びに情報処理方法 |
| EP1921156A1 (en) | 2006-11-10 | 2008-05-14 | bioMerieux B.V. | Improved multiplex nucleic acid amplification using blocked primers |
| EP2644707B1 (en) | 2008-04-30 | 2015-06-03 | Integrated Dna Technologies, Inc. | RNase-H-based assays utilizing modified RNA monomers |
| WO2011060014A1 (en) | 2009-11-13 | 2011-05-19 | Integrated Dna Technologies, Inc. | Small rna detection assays |
| US8618253B2 (en) | 2010-05-25 | 2013-12-31 | Samsung Techwin Co., Ltd. | Modified RNAse H and detection of nucleic acid amplification |
| US20120052495A1 (en) | 2010-08-30 | 2012-03-01 | Samsung Techwin Co., Ltd. | Oligonucleotides for detecting listeria monocytogenes and use thereof |
| US20120052501A1 (en) | 2010-08-30 | 2012-03-01 | Samsung Techwin Co., Ltd. | Kit for detecting htlv strains and use thereof |
-
2009
- 2009-04-30 EP EP13173388.3A patent/EP2644707B1/en active Active
- 2009-04-30 US US12/433,896 patent/US20090325169A1/en not_active Abandoned
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- 2009-04-30 AU AU2009242546A patent/AU2009242546B2/en active Active
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- 2009-04-30 CA CA2722541A patent/CA2722541C/en active Active
- 2009-04-30 EP EP13173391.7A patent/EP2644710B1/en active Active
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