JP5539325B2 - 修飾されたrna単量体を用いるrnアーゼhを基礎とするアッセイ - Google Patents

修飾されたrna単量体を用いるrnアーゼhを基礎とするアッセイ Download PDF

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JP5539325B2
JP5539325B2 JP2011507669A JP2011507669A JP5539325B2 JP 5539325 B2 JP5539325 B2 JP 5539325B2 JP 2011507669 A JP2011507669 A JP 2011507669A JP 2011507669 A JP2011507669 A JP 2011507669A JP 5539325 B2 JP5539325 B2 JP 5539325B2
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primer
rnase
cleavage
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dna
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JP2011521624A (ja
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ウオルダー,ジヨゼフ・アラン
ベールケ,マーク・アーロン
ローズ,スコツト
ドボシー,ジヨゼフ
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インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates

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JP2011507669A 2008-04-30 2009-04-30 修飾されたrna単量体を用いるrnアーゼhを基礎とするアッセイ Active JP5539325B2 (ja)

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US4920408P 2008-04-30 2008-04-30
US61/049,204 2008-04-30
PCT/US2009/042454 WO2009135093A2 (en) 2008-04-30 2009-04-30 Rnase-h-based assays utilizing modified rna monomers

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JP2013185104A Pending JP2014014371A (ja) 2008-04-30 2013-09-06 修飾されたrna単量体を用いるrnアーゼhを基礎とするアッセイ
JP2015116458A Ceased JP2015221036A (ja) 2008-04-30 2015-06-09 修飾されたrna単量体を用いるrnアーゼhを基礎とするアッセイ
JP2016246235A Active JP6453296B2 (ja) 2008-04-30 2016-12-20 修飾されたrna単量体を用いるrnアーゼhを基礎とするアッセイ
JP2016246236A Expired - Fee Related JP6276835B2 (ja) 2008-04-30 2016-12-20 修飾されたrna単量体を用いるrnアーゼhを基礎とするアッセイ
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JP2015116458A Ceased JP2015221036A (ja) 2008-04-30 2015-06-09 修飾されたrna単量体を用いるrnアーゼhを基礎とするアッセイ
JP2016246235A Active JP6453296B2 (ja) 2008-04-30 2016-12-20 修飾されたrna単量体を用いるrnアーゼhを基礎とするアッセイ
JP2016246236A Expired - Fee Related JP6276835B2 (ja) 2008-04-30 2016-12-20 修飾されたrna単量体を用いるrnアーゼhを基礎とするアッセイ
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Families Citing this family (77)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10227641B2 (en) 2008-04-30 2019-03-12 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
EP2644707B1 (en) 2008-04-30 2015-06-03 Integrated Dna Technologies, Inc. RNase-H-based assays utilizing modified RNA monomers
WO2014143228A1 (en) * 2013-03-15 2014-09-18 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
EP3249053A1 (en) 2009-03-27 2017-11-29 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
JP2013507910A (ja) * 2009-10-13 2013-03-07 シンテッツァ モレキュラー ディテクション イスラエル リミテッド 核酸試料から標的配列を増幅するための方法および組成物
WO2011060014A1 (en) 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
JP2011188855A (ja) * 2010-03-11 2011-09-29 Samsung Techwin Co Ltd 切断可能なキメラプローブを使用したサルモネラのリアルタイム検出方法
US8618253B2 (en) * 2010-05-25 2013-12-31 Samsung Techwin Co., Ltd. Modified RNAse H and detection of nucleic acid amplification
US9850527B2 (en) 2010-06-14 2017-12-26 National University Of Singapore Modified stem-loop oligonucleotide mediated reverse transcription and base-spacing constrained quantitative PCR
CA2810856C (en) 2010-08-13 2022-05-17 Envirologix Inc. Compositions and methods for quantifying a nucleic acid sequence in a sample
US20120052482A1 (en) * 2010-08-27 2012-03-01 Samsung Techwin Co., Ltd. Kit for detecting hepatitis c virus and method of detecting hepatitis c virus using the same
US20120052495A1 (en) * 2010-08-30 2012-03-01 Samsung Techwin Co., Ltd. Oligonucleotides for detecting listeria monocytogenes and use thereof
US20120052501A1 (en) * 2010-08-30 2012-03-01 Samsung Techwin Co., Ltd. Kit for detecting htlv strains and use thereof
US20120052492A1 (en) * 2010-08-30 2012-03-01 Samsung Techwin Co., Ltd. Oligonucleotides for detecting listeria spp. and use thereof
US20120052500A1 (en) * 2010-08-30 2012-03-01 Samsung Techwin Co., Ltd. Kit for detecting chlamydia trachomatis strains and method for detecting chlamydia trachomatis strains using the same
US20120052503A1 (en) * 2010-08-30 2012-03-01 Samsung Techwin Co., Ltd. Kit for detecting neisseria gonorrhoeae strains and method for detecting neisseria gonorrhoeae strains using the same
US20120088246A1 (en) * 2010-10-07 2012-04-12 Samsung Techwin Co., Ltd. Real time pcr detection of single nucleotide polymorphisms
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
EP2663654A1 (en) * 2011-01-14 2013-11-20 Life Technologies Corporation Methods, compositions, and kits for detecting rare cells
WO2012106546A2 (en) 2011-02-02 2012-08-09 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
WO2012135053A2 (en) * 2011-03-25 2012-10-04 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
JP2014518071A (ja) * 2011-06-15 2014-07-28 エヌエスイー プロダクツ インコーポレイテッド カロリー制限およびカロリー制限模倣物の同定用マーカー
US9603949B2 (en) 2011-09-01 2017-03-28 University Of Iowa Research Foundation Oligonucleotide-based probes for detection of bacterial nucleases
WO2013123238A1 (en) 2012-02-14 2013-08-22 Great Basin Scientific Methods of isothermal amplification using blocked primers
BR112014025262A8 (pt) 2012-04-09 2021-06-29 Envirologix Inc “métodos para quantificar um produto específico em uma reação de amplificação de extensão e corte, para detectar uma pluralidade de produtos de reação distintos produzidos ao longo de uma única reação, para monitorar em tempo real uma reação de amplificação de extensão e corte, para monitorar em tempo real uma molécula-alvo de ácido nucleico em uma reação de amplificação de extensão e corte, para monitorar em tempo real uma molécula-alvo de ácido nucleico em uma amostra de teste, e para amplificar um polinucleotídeo, kit, e, oligonucleotídeo isolado
US20130310269A1 (en) * 2012-05-04 2013-11-21 Austin So Hot-start digital pcr
DK2855692T3 (da) 2012-05-24 2019-10-14 Univ Utah Res Found Ekstrem pcr
WO2014018093A1 (en) * 2012-07-26 2014-01-30 Illumina, Inc. Compositions and methods for the amplification of nucleic acids
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
AU2014205110A1 (en) 2013-01-13 2015-08-27 Unitaq Bio Methods and compositions for PCR using blocked and universal primers
WO2014134144A1 (en) * 2013-02-28 2014-09-04 The General Hospital Corporation Mirna profiling compositions and methods of use
EP2970951B1 (en) 2013-03-13 2019-02-20 Illumina, Inc. Methods for nucleic acid sequencing
US10119134B2 (en) 2013-03-15 2018-11-06 Abvitro Llc Single cell bar-coding for antibody discovery
EP2971160B1 (en) 2013-03-15 2018-05-30 Bio-Rad Laboratories, Inc. Digital assays for mutation detection
CA2930595C (en) * 2013-11-14 2023-09-19 Integrated Dna Technologies, Inc. Dna polymerase mutants having enhanced template discrimination activity
US10385388B2 (en) 2013-12-06 2019-08-20 Swift Biosciences, Inc. Cleavable competitor polynucleotides
AU2014364926B2 (en) 2013-12-20 2017-12-14 Illumina, Inc. Preserving genomic connectivity information in fragmented genomic DNA samples
CA2939124C (en) * 2014-02-07 2023-08-29 University Of Iowa Research Foundation Oligonucleotide-based probes and methods for detection of microbes
GB201403216D0 (en) * 2014-02-24 2014-04-09 Cambridge Epigenetix Ltd Nucleic acid sample preparation
WO2015164494A1 (en) 2014-04-22 2015-10-29 Envirologix, Inc. Compositions and methods for enhancing and/or predicting dna amplification
KR102465287B1 (ko) * 2014-08-11 2022-11-09 루미넥스 코포레이션 핵산 검정에서 개선된 용융 판별 및 멀티플렉싱을 위한 프로브
US20160115473A1 (en) * 2014-08-14 2016-04-28 Abbott Molecular Inc. Multifunctional oligonucleotides
SG10201911069WA (en) 2014-09-15 2020-01-30 Abvitro Llc High-throughput nucleotide library sequencing
KR102472027B1 (ko) 2014-10-17 2022-11-30 일루미나 케임브리지 리미티드 근접 보존 전위
US10793922B2 (en) 2014-10-20 2020-10-06 Envirologix Inc. Compositions and methods for detecting an RNA virus
WO2016081551A1 (en) 2014-11-20 2016-05-26 Ampliwise Inc. Compositions and methods for nucleic acid amplification
US10619199B2 (en) * 2015-03-06 2020-04-14 Sigma-Aldrich Co. Llc Nucleic acid amplification and library preparation
AU2016313095A1 (en) * 2015-08-24 2017-11-23 Qiagen Gmbh Method for generating a RNA-sequencing library
WO2018057051A1 (en) 2016-09-24 2018-03-29 Abvitro Llc Affinity-oligonucleotide conjugates and uses thereof
WO2017053903A1 (en) 2015-09-24 2017-03-30 Abvitro Llc Single amplicon activated exclusion pcr
MY193806A (en) 2015-09-24 2022-10-27 Abvitro Llc Affinity-oligonucleotide conjugates and uses thereof
AU2016326734B2 (en) 2015-09-25 2022-07-07 Abvitro Llc High throughput process for T cell receptor target identification of natively-paired T cell receptor sequences
ES2916153T3 (es) * 2015-11-05 2022-06-28 Univ Utah Res Found PCR extrema para transcripción inversa
US20210395799A1 (en) * 2015-11-25 2021-12-23 Integrated Dna Technologies, Inc. Methods for variant detection
CN116064748A (zh) * 2015-11-25 2023-05-05 综合基因技术公司 用于变体检测的方法
WO2017136387A1 (en) * 2016-02-01 2017-08-10 Integrated Dna Technologies, Inc. Cleavable primers for isothermal amplification
CN107083427B (zh) * 2016-04-01 2021-07-30 广州市基准医疗有限责任公司 Dna连接酶介导的dna扩增技术
US11268117B2 (en) 2016-06-10 2022-03-08 Life Technologies Corporation Methods and compositions for nucleic acid amplification
EP3610030B1 (en) 2017-04-13 2024-08-07 Integrated DNA Technologies, Inc. Methods for variant detection
CN111148849A (zh) 2017-05-26 2020-05-12 阿布维托有限责任公司 高通量多核苷酸文库测序和转录组分析
US10081829B1 (en) 2017-06-13 2018-09-25 Genetics Research, Llc Detection of targeted sequence regions
WO2018231985A1 (en) * 2017-06-13 2018-12-20 Genetics Research, Llc, D/B/A Zs Genetics, Inc. Polynucleic acid molecule enrichment methodologies
US10527608B2 (en) 2017-06-13 2020-01-07 Genetics Research, Llc Methods for rare event detection
US20200292526A1 (en) 2017-09-07 2020-09-17 Juno Therapeutics, Inc. Methods of identifying cellular attributes related to outcomes associated with cell therapy
JP7421202B2 (ja) 2017-09-29 2024-01-24 ユニバーシティー オブ アイオワ リサーチ ファンデーション デジタルヌクレアーゼ検出組成物および方法
CN112513265A (zh) * 2018-05-22 2021-03-16 博诚研究中心 用于人类癌症检测的修饰核酸的靶向富集和测序
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
CN109913538A (zh) * 2018-09-06 2019-06-21 湖南融健基因生物科技有限公司 一种快速检测snp位点的荧光定量试剂盒
US11591629B2 (en) * 2019-12-23 2023-02-28 Cheng-Yao Chen Method and kit for template-independent nucleic acid synthesis
KR20210109745A (ko) * 2020-02-28 2021-09-07 주식회사 누리바이오 단일 표적 유전자의 유전적 변이 실시간 검출용 단일핵산 및 이를 이용한 검출 방법
WO2021259201A1 (zh) * 2020-06-22 2021-12-30 苏州新海生物科技股份有限公司 一种核酸配体及其应用
CN113897420A (zh) * 2020-06-22 2022-01-07 上海思路迪生物医学科技有限公司 基于扩增子测序的大片段重排检测的引物组合物、试剂盒和检测方法
CN112063689A (zh) * 2020-08-21 2020-12-11 上海思路迪生物医学科技有限公司 一种高效rna反转录方法及rna和dna同步建库方法
CN113981069B (zh) * 2021-11-10 2023-08-15 郑州华沃生物科技有限公司 检测adrb1基因g1165c多态性的引物、试剂盒及其检测方法和应用
CN115948388A (zh) * 2022-12-30 2023-04-11 纳昂达(南京)生物科技有限公司 特异性捕获引物、靶向捕获探针组合物、靶向捕获文库的构建方法及应用
WO2025096569A1 (en) * 2023-10-30 2025-05-08 The Penn State Research Foundation Methods and compositions for real-time antimicrobial resistance profiling
WO2025207561A1 (en) * 2024-03-27 2025-10-02 Cepheid Isothermal amplification using novel synthetic oligonucleotides

Family Cites Families (85)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
CA1340522C (en) 1987-03-10 1999-05-04 Heinz Dobeli Fusion proteins containing neighbouring histidines for improved purification
US6031091A (en) 1987-09-21 2000-02-29 Gen-Probe Incorporated Non-nucleotide linking reagents for nucleotide probes
US5403711A (en) 1987-11-30 1995-04-04 University Of Iowa Research Foundation Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved
IE61148B1 (en) 1988-03-10 1994-10-05 Ici Plc Method of detecting nucleotide sequences
US5002867A (en) 1988-04-25 1991-03-26 Macevicz Stephen C Nucleic acid sequence determination by multiple mixed oligonucleotide probes
JP2955759B2 (ja) 1988-07-20 1999-10-04 セゲブ・ダイアグノスティックス・インコーポレイテッド 核酸配列を増幅及び検出する方法
US5137806A (en) 1989-12-11 1992-08-11 Board Of Regents, The University Of Texas System Methods and compositions for the detection of sequences in selected DNA molecules
US5516663A (en) * 1990-01-26 1996-05-14 Abbott Laboratories Ligase chain reaction with endonuclease IV correction and contamination control
ES2141088T3 (es) 1990-02-16 2000-03-16 Hoffmann La Roche Mejoras en la especificidad y conveniencia de la reaccion en cadena de la polimerasa.
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
JPH0515439A (ja) 1991-07-08 1993-01-26 Matsushita Electric Ind Co Ltd ジヤー炊飯器の制御回路
EP0655090B1 (en) 1992-04-27 2000-12-27 The Trustees Of Dartmouth College Detection of gene sequences in biological fluids
CA2135073C (en) 1992-05-06 2002-11-19 Daniel L. Kacian Nucleic acid sequence amplification method, composition and kit
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
ATE206764T1 (de) * 1994-07-16 2001-10-15 Roche Diagnostics Gmbh Verfahren zum sensitiven nachweis von nukleinsäuren
FR2724934B1 (fr) * 1994-09-26 1997-01-24 Bio Merieux Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique
WO1996015271A1 (en) * 1994-11-16 1996-05-23 Abbott Laboratories Multiplex ligations-dependent amplification
US20030165908A1 (en) 1994-12-30 2003-09-04 Georgetown University Fluorometric assay for detecting nucleic acid cleavage
WO1996021144A1 (en) 1994-12-30 1996-07-11 Georgetown University Fluorometric assay for detecting nucleic acid cleavage
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US5898031A (en) 1996-06-06 1999-04-27 Isis Pharmaceuticals, Inc. Oligoribonucleotides for cleaving RNA
EP0912767B1 (en) 1996-07-16 2006-09-13 Gen-Probe Incorporated Methods for detecting and amplifying nucleic acid sequences using modified oligonucleotides having increased target specific tm
US5853990A (en) * 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
EP0866071B1 (en) 1997-03-20 2004-10-20 F. Hoffmann-La Roche Ag Modified primers
WO1998046793A1 (en) 1997-04-16 1998-10-22 Trevigen, Inc. Detection and mapping of point mutations using partial digestion
US5846726A (en) 1997-05-13 1998-12-08 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
WO1999002728A1 (en) 1997-07-11 1999-01-21 Brax Group Limited Characterising nucleic acids
JPH1132772A (ja) * 1997-07-24 1999-02-09 Mitsubishi Chem Corp 耐熱性リボヌクレアーゼh及びそれをコードするdna
GB9725237D0 (en) 1997-11-29 1998-01-28 Secr Defence Amplification system
WO2005021776A2 (en) * 2003-08-21 2005-03-10 Isis Pharmaceuticals, Inc. METHODS OF USING MAMMALIAN RNase H AND COMPOSITIONS THEREOF
EP1036165A4 (en) 1997-12-04 2003-05-14 Isis Pharmaceuticals Inc HUMAN RNASE-H AND COMPOSITIONS AND USES THEREOF
US6248877B1 (en) 1998-04-07 2001-06-19 Biolink Partners Solid phase synthesis of organic compounds via phosphitylating reagents
AU3174600A (en) * 1999-03-10 2000-09-28 Asm Scientific, Inc. A method for direct nucleic acid sequencing
IL148091A0 (en) 1999-09-13 2002-09-12 Nugen Technologies Inc Methods and compositions for linear isothermal amplification of polynucleotide sequences
US6274353B1 (en) * 1999-09-22 2001-08-14 Genecopoeia, Inc. Method and compositions for improved polynucleotide synthesis
DE60027040T2 (de) 1999-10-29 2006-11-23 Stratagene California, La Jolla Zusammensetzungen und methoden zur verwendung von dna polymerasen
WO2001061015A2 (en) 2000-02-17 2001-08-23 Qiagen Gmbh Thermostable chimeric nucleic acid polymerases and uses thereof
US7033763B2 (en) 2000-02-23 2006-04-25 City Of Hope Pyrophosphorolysis activated polymerization (PAP)
CA2395391C (en) 2000-02-23 2011-08-02 City Of Hope Pyrophosphorolysis activated polymerization (pap): application to allele-specific amplification and nucleic acid sequence determination
US6596490B2 (en) 2000-07-14 2003-07-22 Applied Gene Technologies, Inc. Nucleic acid hairpin probes and uses thereof
EP1318197B1 (en) 2000-09-14 2008-10-15 Takara Bio Inc. Thermotolerant ribonuclease h
CA2430329A1 (en) 2000-12-13 2002-06-20 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
CN100351393C (zh) 2001-02-15 2007-11-28 宝生物工程株式会社 核苷酸多态性的检测方法
US6596489B2 (en) 2001-03-30 2003-07-22 Applied Gene Technologies Methods and compositions for analyzing nucleotide sequence mismatches using RNase H
EP1275735A1 (en) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Composition and method for hot start nucleic acid amplification
EP1420069A4 (en) 2001-08-20 2005-11-02 Takara Bio Inc nucleic acid amplification
CA2409775C (en) * 2001-12-03 2010-07-13 F. Hoffmann-La Roche Ag Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro
AU2003213652A1 (en) * 2002-03-01 2003-09-16 Integrated Dna Technologies, Inc. Polynomial amplification of nucleic acids
US7629152B2 (en) 2002-03-01 2009-12-08 Integrated Dna Technologies, Inc. Methods for amplifying polymeric nucleic acids
SI3363809T1 (sl) * 2002-08-23 2020-08-31 Illumina Cambridge Limited Modificirani nukleotidi za polinukleotidno sekvenciranje
JP4105693B2 (ja) * 2002-08-30 2008-06-25 タカラバイオ株式会社 耐熱性リボヌクレアーゼh
ATE411400T1 (de) 2002-11-21 2008-10-15 Epict Technologies Verfahren zur verwendung von riboprimern zur strangverdrängungsreplikation von zielsequenzen
US20040175737A1 (en) * 2002-12-23 2004-09-09 Wyeth Assay for RNase H activity
JP2005006587A (ja) * 2003-06-20 2005-01-13 Takara Bio Inc 標的核酸の増幅及び/又は検出方法
AU2004203649B2 (en) 2003-08-12 2006-01-12 F. Hoffmann-La Roche Ag Thermostable Taq polymerase fragment
WO2005049849A2 (en) 2003-11-14 2005-06-02 Integrated Dna Technologies, Inc. Fluorescence quenching azo dyes, their methods of preparation and use
KR101041106B1 (ko) 2003-11-25 2011-06-13 한면기 핵산과 단백질의 신규한 실시간 탐지방법
WO2007062495A1 (en) * 2005-11-30 2007-06-07 Roy Rabindranauth Sooknanan Selective terminal tagging of nucleic acids
US7344834B2 (en) 2003-12-08 2008-03-18 Cytyc Corporation Method for DNA amplification using DNA blocking probes
JP4249186B2 (ja) * 2003-12-10 2009-04-02 タカラバイオ株式会社 核酸の増幅方法
WO2005123913A2 (en) * 2004-06-09 2005-12-29 Nustar Laboratory Reversibly modified thermostable enzyme compositions and methods of making and using the same
US20060110765A1 (en) 2004-11-23 2006-05-25 Wang Xiao B Detection of nucleic acid variation by cleavage-amplification (CleavAmp) method
WO2006070666A1 (ja) * 2004-12-28 2006-07-06 Takara Bio Inc. 遺伝子多型の同時検出方法
WO2006074233A2 (en) * 2005-01-06 2006-07-13 Applera Corporation Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification
WO2006081426A2 (en) * 2005-01-28 2006-08-03 Applera Corporation Compositions and methods for terminating a sequencing reaction at a specific location in a target dna template
EP1844162B1 (en) 2005-02-01 2014-10-15 Applied Biosystems, LLC Method for identifying a sequence in a polynucleotide
US20060211024A1 (en) 2005-03-10 2006-09-21 Gwc Technologies Incorporated Methods for analysis of a nucleic acid sample
US20090068643A1 (en) 2005-11-23 2009-03-12 Integrated Dna Technologies, Inc. Dual Function Primers for Amplifying DNA and Methods of Use
WO2007103558A2 (en) 2006-03-09 2007-09-13 The Regents Of The University Of California Hybrid energy transfer for nucleic acid detection
WO2007106907A2 (en) 2006-03-15 2007-09-20 Integrated Dna Technologies, Inc. Nucleic acid monomers with 2'-chemical moieties
DE602007013223D1 (de) 2006-06-01 2011-04-28 Trilink Biotechnologies San Diego Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation
GB0611444D0 (en) * 2006-06-09 2006-07-19 Medical Res Council Technology Rnase H2 complex and genes therefor
AU2007260707A1 (en) * 2006-06-16 2007-12-21 Pacific Biosciences Of California, Inc. Controlled initiation of primer extension
JP2008072427A (ja) 2006-09-14 2008-03-27 Konica Minolta Business Technologies Inc 画像形成装置及びクライアント/サーバ型情報処理システム並びに情報処理方法
EP1921156A1 (en) 2006-11-10 2008-05-14 bioMerieux B.V. Improved multiplex nucleic acid amplification using blocked primers
EP2644707B1 (en) 2008-04-30 2015-06-03 Integrated Dna Technologies, Inc. RNase-H-based assays utilizing modified RNA monomers
WO2011060014A1 (en) 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
US8618253B2 (en) 2010-05-25 2013-12-31 Samsung Techwin Co., Ltd. Modified RNAse H and detection of nucleic acid amplification
US20120052495A1 (en) 2010-08-30 2012-03-01 Samsung Techwin Co., Ltd. Oligonucleotides for detecting listeria monocytogenes and use thereof
US20120052501A1 (en) 2010-08-30 2012-03-01 Samsung Techwin Co., Ltd. Kit for detecting htlv strains and use thereof

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