JP2011500092A - 非ランダムプライマーを用いたｃＤＮＡ合成の方法 - Google Patents

非ランダムプライマーを用いたｃＤＮＡ合成の方法 Download PDF

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JP2011500092A
JP2011500092A JP2010531293A JP2010531293A JP2011500092A JP 2011500092 A JP2011500092 A JP 2011500092A JP 2010531293 A JP2010531293 A JP 2010531293A JP 2010531293 A JP2010531293 A JP 2010531293A JP 2011500092 A JP2011500092 A JP 2011500092A
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population
oligonucleotides
nucleic acid
hybridizing
rna
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JP2011500092A5 (enExample
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クリストファー ケイ． レイモンド
クリストファー アーマー
ジョン キャッスル
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Rosetta Inpharmatics LLC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
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  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
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  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2010531293A 2007-10-26 2008-10-24 非ランダムプライマーを用いたｃＤＮＡ合成の方法 Pending JP2011500092A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US98308507P 2007-10-26 2007-10-26
PCT/US2008/081206 WO2009055732A1 (en) 2007-10-26 2008-10-24 Cdna synthesis using non-random primers

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JP2011500092A true JP2011500092A (ja) 2011-01-06
JP2011500092A5 JP2011500092A5 (enExample) 2012-12-20

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US (3) US20100029511A1 (enExample)
EP (1) EP2209912A1 (enExample)
JP (1) JP2011500092A (enExample)
CN (1) CN102124126A (enExample)
WO (1) WO2009055732A1 (enExample)

Cited By (2)

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JP2019513363A (ja) * 2016-03-31 2019-05-30 バークレー ライツ，インコーポレイテッド 核酸安定化試薬、キット、及びその使用方法
US11280028B1 (en) 2021-02-24 2022-03-22 Agency For Science, Technology And Research (A*Star) Unbiased and simultaneous amplification method for preparing a double-stranded DNA library from a sample of more than one type of nucleic acid

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EP2209912A1 (en) * 2007-10-26 2010-07-28 Rosetta Inpharmatics LLC Cdna synthesis using non-random primers
JP5685085B2 (ja) * 2008-01-14 2015-03-18 アプライド バイオシステムズ リミテッド ライアビリティー カンパニー リボ核酸を検出するための組成物、方法およびキット
WO2011032053A1 (en) * 2009-09-11 2011-03-17 Nugen Technologies, Inc. Compositions and methods for whole transcriptome analysis
WO2012064739A2 (en) * 2010-11-08 2012-05-18 The Trustees Of Columbia University In The City Of New York Microbial enrichment primers
WO2012174214A1 (en) * 2011-06-15 2012-12-20 The Regents Of The University Of California High resolution analysis of mammalian transcriptome using gene pool specific primers
EP2769007B1 (en) 2011-10-19 2016-12-07 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
CN105861487B (zh) 2012-01-26 2020-05-05 纽亘技术公司 用于靶向核酸序列富集和高效文库产生的组合物和方法
WO2013165598A1 (en) * 2012-04-30 2013-11-07 The Research Foundation For Suny Cancer blood test using bc200 rna isolated from peripheral blood for diagnosis and treatment of invasive breast cancer
CA2877094A1 (en) * 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
CN114891871A (zh) 2012-08-14 2022-08-12 10X基因组学有限公司 微胶囊组合物及方法
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
GB201301857D0 (en) * 2013-02-01 2013-03-20 Selvi Ozan Method
EP2971130A4 (en) 2013-03-15 2016-10-05 Nugen Technologies Inc SEQUENTIAL SEQUENCING
JP6525473B2 (ja) 2013-11-13 2019-06-05 ニューゲン テクノロジーズ， インコーポレイテッド 複製物配列決定リードを同定するための組成物および方法
JP6416939B2 (ja) 2014-02-13 2018-10-31 タカラ バイオ ユーエスエー，インコーポレイティド 核酸の初期収集物から標的分子を減損させる方法、並びにそれを実施するための組成物及びキット
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
EP4574974A3 (en) * 2014-06-26 2025-10-08 10x Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
WO2016154455A1 (en) * 2015-03-24 2016-09-29 Sigma-Aldrich Co. Llc Directional amplification of rna
CN105985949A (zh) * 2015-11-02 2016-10-05 中国动物卫生与流行病学中心 一种rna高通量测序文库构建方法
WO2017140659A1 (en) * 2016-02-15 2017-08-24 F. Hoffmann-La Roche Ag System and method for targeted depletion of nucleic acids
US10472666B2 (en) 2016-02-15 2019-11-12 Roche Sequencing Solutions, Inc. System and method for targeted depletion of nucleic acids
CN105925675B (zh) * 2016-04-26 2020-06-05 序康医疗科技（苏州）有限公司 扩增dna的方法
GB201621477D0 (en) * 2016-12-16 2017-02-01 Multiplicom Nv Modified multiplex and multistep amplification reactions and reagents therefor
WO2018165593A1 (en) * 2017-03-09 2018-09-13 iRepertoire, Inc. Dimer avoided multiplex polymerase chain reaction for amplification of multiple targets
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
JP2021522815A (ja) * 2018-05-07 2021-09-02 ロシュ イノベーション センター コペンハーゲン エーエス 超並列シーケンシングを用いるｌｎａオリゴヌクレオチド治療法の品質管理法
WO2020124391A1 (zh) * 2018-12-18 2020-06-25 深圳先进技术研究院 骨密度性状遗传力分析方法及装置
WO2020184551A1 (ja) 2019-03-13 2020-09-17 東洋紡株式会社 核酸の生成および増幅
CN111534512A (zh) * 2019-09-11 2020-08-14 广东美格基因科技有限公司 一种去除核糖体rna的反转录引物池、试剂盒及去除核糖体rna的方法
EP3851542A1 (en) 2020-01-20 2021-07-21 Tecan Genomics, Inc. Depletion of abundant uninformative sequences
CN113275053A (zh) 2020-02-03 2021-08-20 帝肯基因组学公司 试剂存储系统
JPWO2024157925A1 (enExample) 2023-01-26 2024-08-02

Citations (1)

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WO2007050990A2 (en) * 2005-10-27 2007-05-03 Rosetta Inpharmatics Llc Nucleic acid amplification using non-random primers

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AU4174397A (en) * 1996-08-30 1998-03-19 Life Technologies, Inc. Methods for identification and isolation of specific nucleotide sequences in cdna and genomic dna
WO1999011823A2 (en) * 1997-09-05 1999-03-11 Sidney Kimmel Cancer Center Selection of pcr primer pairs to amplify a group of nucleotide sequences
US6787308B2 (en) * 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
MXPA02012739A (es) * 2001-03-09 2004-04-20 Nugen Technologies Inc Metodos y composiciones para amplificacion de secuencias de arn.
WO2003020979A1 (en) * 2001-08-31 2003-03-13 Rosetta Inpharmactis Llc. Methods for preparing nucleic acid samples
EP2209912A1 (en) * 2007-10-26 2010-07-28 Rosetta Inpharmatics LLC Cdna synthesis using non-random primers

Patent Citations (1)

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Publication number Priority date Publication date Assignee Title
WO2007050990A2 (en) * 2005-10-27 2007-05-03 Rosetta Inpharmatics Llc Nucleic acid amplification using non-random primers

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019513363A (ja) * 2016-03-31 2019-05-30 バークレー ライツ，インコーポレイテッド 核酸安定化試薬、キット、及びその使用方法
JP7019590B2 (ja) 2016-03-31 2022-02-15 バークレー ライツ，インコーポレイテッド 核酸安定化試薬、キット、及びその使用方法
JP2022064981A (ja) * 2016-03-31 2022-04-26 バークレー ライツ，インコーポレイテッド 核酸安定化試薬、キット、及びその使用方法
US11999992B2 (en) 2016-03-31 2024-06-04 Bruker Cellular Analysis, Inc. Nucleic acid stabilization reagent, kits, and methods of use thereof
US11280028B1 (en) 2021-02-24 2022-03-22 Agency For Science, Technology And Research (A*Star) Unbiased and simultaneous amplification method for preparing a double-stranded DNA library from a sample of more than one type of nucleic acid

Also Published As

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US20130252823A1 (en) 2013-09-26
EP2209912A1 (en) 2010-07-28
US20100029511A1 (en) 2010-02-04
CN102124126A (zh) 2011-07-13
US20110039732A1 (en) 2011-02-17
WO2009055732A1 (en) 2009-04-30

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