JP2010004884A5 - - Google Patents
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- Publication number
- JP2010004884A5 JP2010004884A5 JP2009151195A JP2009151195A JP2010004884A5 JP 2010004884 A5 JP2010004884 A5 JP 2010004884A5 JP 2009151195 A JP2009151195 A JP 2009151195A JP 2009151195 A JP2009151195 A JP 2009151195A JP 2010004884 A5 JP2010004884 A5 JP 2010004884A5
- Authority
- JP
- Japan
- Prior art keywords
- reagent
- dna
- nucleic acid
- abasic
- polyamine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000003153 chemical reaction reagent Substances 0.000 claims 16
- 208000035657 Abasia Diseases 0.000 claims 9
- 108020004707 nucleic acids Proteins 0.000 claims 9
- 150000007523 nucleic acids Chemical class 0.000 claims 9
- 102000039446 nucleic acids Human genes 0.000 claims 9
- 238000000034 method Methods 0.000 claims 6
- 229920000768 polyamine Polymers 0.000 claims 6
- 238000003752 polymerase chain reaction Methods 0.000 claims 6
- 230000003321 amplification Effects 0.000 claims 5
- 230000000694 effects Effects 0.000 claims 5
- 238000003199 nucleic acid amplification method Methods 0.000 claims 5
- 230000015556 catabolic process Effects 0.000 claims 4
- 238000006731 degradation reaction Methods 0.000 claims 4
- 108020001738 DNA Glycosylase Proteins 0.000 claims 3
- 102000028381 DNA glycosylase Human genes 0.000 claims 3
- 238000011109 contamination Methods 0.000 claims 3
- 239000003155 DNA primer Substances 0.000 claims 2
- AHCYMLUZIRLXAA-SHYZEUOFSA-N Deoxyuridine 5'-triphosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 AHCYMLUZIRLXAA-SHYZEUOFSA-N 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 2
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 claims 2
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 claims 2
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 claims 2
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 claims 2
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 claims 2
- 239000002777 nucleoside Substances 0.000 claims 2
- -1 nucleoside triphosphate Chemical class 0.000 claims 2
- 239000011541 reaction mixture Substances 0.000 claims 2
- 150000003839 salts Chemical class 0.000 claims 2
- 239000012488 sample solution Substances 0.000 claims 2
- ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 claims 2
- PFNFFQXMRSDOHW-UHFFFAOYSA-N spermine Chemical compound NCCCNCCCCNCCCN PFNFFQXMRSDOHW-UHFFFAOYSA-N 0.000 claims 2
- XFNJVJPLKCPIBV-UHFFFAOYSA-N trimethylenediamine Chemical compound NCCCN XFNJVJPLKCPIBV-UHFFFAOYSA-N 0.000 claims 2
- 235000011178 triphosphate Nutrition 0.000 claims 2
- 239000001226 triphosphate Substances 0.000 claims 2
- VILCJCGEZXAXTO-UHFFFAOYSA-N 2,2,2-tetramine Chemical compound NCCNCCNCCN VILCJCGEZXAXTO-UHFFFAOYSA-N 0.000 claims 1
- 230000000593 degrading effect Effects 0.000 claims 1
- 229940063673 spermidine Drugs 0.000 claims 1
- 229940063675 spermine Drugs 0.000 claims 1
- 229960001124 trientine Drugs 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US7611108P | 2008-06-26 | 2008-06-26 | |
| US61/076,111 | 2008-06-26 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010004884A JP2010004884A (ja) | 2010-01-14 |
| JP2010004884A5 true JP2010004884A5 (enExample) | 2012-03-08 |
| JP5596308B2 JP5596308B2 (ja) | 2014-09-24 |
Family
ID=41136785
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009151195A Active JP5596308B2 (ja) | 2008-06-26 | 2009-06-25 | 核酸増幅技術におけるキャリーオーバー汚染の防止のための改良方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US8669061B2 (enExample) |
| EP (1) | EP2138590B1 (enExample) |
| JP (1) | JP5596308B2 (enExample) |
| CN (1) | CN101613730B (enExample) |
| CA (1) | CA2670300C (enExample) |
| ES (1) | ES2386023T3 (enExample) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8669061B2 (en) | 2008-06-26 | 2014-03-11 | Roche Molecular Systems, Inc. | Method for the prevention of carryover contamination in nucleic acid amplification technologies |
| WO2013022779A1 (en) * | 2011-08-05 | 2013-02-14 | Ibis Biosciences, Inc. | Analysis of genetic biomarkers for forensic analysis and fingerprinting |
| ES2449665B2 (es) * | 2012-09-19 | 2014-07-17 | Servizo Galego De Saúde-Conselleria De Sanidade, Xunta De Galicia | Procedimiento para el diagnóstico clínico de una enfermedad infecciosa basada en el empleo de la PCR cuantitativa, oligonucleótidos marcados de 8 a 9 nucleótidos de longitud y la UNG del Bacalao del Atlántico (Gadus morhua). |
| JP2016537003A (ja) * | 2013-11-18 | 2016-12-01 | ルビコン ゲノミクス インコーポレイテッド | バックグラウンド減少のための分解可能なアダプター |
| US10036061B2 (en) * | 2013-12-20 | 2018-07-31 | Roche Molecular Systems, Inc. | Oligonucleotide inhibitor of DNA polymerases |
| JP6674905B2 (ja) * | 2014-06-05 | 2020-04-01 | キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング | Ｄｎａ増幅反応の最適化 |
| EP3690060B1 (en) | 2014-10-08 | 2021-12-08 | Cornell University | Method for identification and relative quantification of nucleic acid sequence expression, splice variant, translocation, copy number, or methylation changes using combined nuclease, ligation, and polymerase reactions with carryover prevention |
| CN104389026A (zh) * | 2014-10-30 | 2015-03-04 | 北京诺禾致源生物信息科技有限公司 | 单细胞转录组测序文库的构建方法及其应用 |
| JP6623324B2 (ja) * | 2015-09-07 | 2019-12-25 | 株式会社ファスマック | 等温増幅反応産物の多項目同時検出方法 |
| ES2855748T3 (es) | 2016-07-12 | 2021-09-24 | Hoffmann La Roche | Enriquecimiento de diana de extensión de cebador |
| WO2018015318A1 (en) | 2016-07-18 | 2018-01-25 | F. Hoffmann-La Roche Ag | Method for generating single-stranded circular dna libraries for single molecule sequencing |
| CN111757934A (zh) | 2017-12-21 | 2020-10-09 | 豪夫迈·罗氏有限公司 | 通过单向双重探针引物延伸的靶标富集 |
| AR123675A1 (es) * | 2019-05-07 | 2023-01-04 | Shanghai Bluecross Medical Science Inst | Sistema mejorado de edición de genes |
| US20220372566A1 (en) | 2019-09-20 | 2022-11-24 | Roche Sequencing Solutions, Inc. | Immune repertoire profiling by primer extension target enrichment |
| JP7633283B2 (ja) | 2020-06-08 | 2025-02-19 | エフ． ホフマン－ラ ロシュ アーゲー | ゲノム内の構造再編成を検出するための方法及び組成物 |
| CN113186037B (zh) * | 2021-04-23 | 2022-12-13 | 山东博弘基因科技有限公司 | 一种核酸清除剂 |
| CN118401675A (zh) * | 2021-10-15 | 2024-07-26 | 凯杰科学有限责任公司 | 制备dna文库的方法及其用途 |
| CN114214395A (zh) * | 2021-12-31 | 2022-03-22 | 上海星耀医学科技发展有限公司 | 一种提高基因检测准确性的核酸扩增方法与试剂盒 |
| WO2024013241A1 (en) | 2022-07-14 | 2024-01-18 | F. Hoffmann-La Roche Ag | Variant allele enrichment by unidirectional dual probe primer extension |
| CN116773291B (zh) * | 2023-03-31 | 2024-04-19 | 艾普拜生物科技(苏州)有限公司 | 一种石蜡切片dna预处理试剂及方法 |
| WO2024222446A1 (zh) * | 2023-04-23 | 2024-10-31 | 南京诺唯赞生物科技股份有限公司 | 一种去除非目标核酸模板的方法 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH089997B2 (ja) | 1987-05-26 | 1996-01-31 | 日立冷熱株式会社 | 多翼送風機 |
| US5683896A (en) | 1989-06-01 | 1997-11-04 | Life Technologies, Inc. | Process for controlling contamination of nucleic acid amplification reactions |
| ATE176002T1 (de) * | 1990-07-24 | 1999-02-15 | Hoffmann La Roche | Verringerung von nicht spezifischer amplifikation während einer (in vitro) nukleinsäure amplifikation unter verwendung von modifizierten nukleinsäure basen |
| CA2073298C (en) * | 1991-07-12 | 2007-04-17 | James L. Hartley | Process for controlling contamination of nucleic acid amplification reactions |
| JP3416981B2 (ja) | 1993-03-30 | 2003-06-16 | 株式会社島津製作所 | 核酸合成法 |
| JPH089997A (ja) | 1994-06-28 | 1996-01-16 | Shimadzu Corp | 核酸合成法およびそれに用いる試薬キット |
| US6413747B1 (en) | 1994-10-03 | 2002-07-02 | Shimadzu Corporation | Enhancement of nucleic acid amplification by the addition of a polyamine |
| US6190865B1 (en) | 1995-09-27 | 2001-02-20 | Epicentre Technologies Corporation | Method for characterizing nucleic acid molecules |
| DE19545320A1 (de) | 1995-12-05 | 1997-06-12 | Boehringer Mannheim Gmbh | Thermolabile Uracil-DNA-Glykosylase, Verfahren zu deren Herstellung und Verwendung zur Entfernung von Uracil aus DNA |
| GB9600384D0 (en) | 1996-01-09 | 1996-03-13 | Nyfotek As | Dna glycosylases |
| JP4629167B2 (ja) | 1997-10-17 | 2011-02-09 | 株式会社島津製作所 | 核酸合成法 |
| EP1038974A1 (de) | 1999-03-26 | 2000-09-27 | Mira Diagnostica GmbH | Verfahren, Oligonucleotide zum Abbau von in-vitro synthetisierten Nucleinsäuremolekülen |
| EP1041159A3 (de) | 1999-03-26 | 2000-10-25 | MIRA Diagnostika GmbH | Verfahren, Oligonucleotide zum Abbau von in-vitro synthetisierten Nucleinsäuremolekülen |
| JP2001008680A (ja) | 1999-06-30 | 2001-01-16 | Shimadzu Corp | 核酸合成法 |
| US20040152094A1 (en) | 2001-05-10 | 2004-08-05 | Steffen Danielsen | Method for producing recombined polynucleotides |
| US20100022403A1 (en) * | 2006-06-30 | 2010-01-28 | Nurith Kurn | Methods for fragmentation and labeling of nucleic acids |
| US8669061B2 (en) | 2008-06-26 | 2014-03-11 | Roche Molecular Systems, Inc. | Method for the prevention of carryover contamination in nucleic acid amplification technologies |
-
2009
- 2009-06-16 US US12/485,569 patent/US8669061B2/en active Active
- 2009-06-24 EP EP09008229A patent/EP2138590B1/en active Active
- 2009-06-24 ES ES09008229T patent/ES2386023T3/es active Active
- 2009-06-25 JP JP2009151195A patent/JP5596308B2/ja active Active
- 2009-06-25 CA CA2670300A patent/CA2670300C/en not_active Expired - Fee Related
- 2009-06-26 CN CN200910146289.7A patent/CN101613730B/zh active Active
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