JP2006166907A5 - - Google Patents
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- Publication number
- JP2006166907A5 JP2006166907A5 JP2005327174A JP2005327174A JP2006166907A5 JP 2006166907 A5 JP2006166907 A5 JP 2006166907A5 JP 2005327174 A JP2005327174 A JP 2005327174A JP 2005327174 A JP2005327174 A JP 2005327174A JP 2006166907 A5 JP2006166907 A5 JP 2006166907A5
- Authority
- JP
- Japan
- Prior art keywords
- concentration
- poly
- reaction
- rna
- adapter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 238000006243 chemical reaction Methods 0.000 claims 12
- 238000000034 method Methods 0.000 claims 10
- 108020004414 DNA Proteins 0.000 claims 7
- 102000053602 DNA Human genes 0.000 claims 7
- 239000012472 biological sample Substances 0.000 claims 5
- 108090000623 proteins and genes Proteins 0.000 claims 4
- 230000006820 DNA synthesis Effects 0.000 claims 3
- 238000010839 reverse transcription Methods 0.000 claims 3
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 claims 2
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 claims 2
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 claims 2
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 claims 2
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 claims 2
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 claims 2
- 229910001425 magnesium ion Inorganic materials 0.000 claims 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims 1
- 108010083359 Antigen Receptors Proteins 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108020004682 Single-Stranded DNA Proteins 0.000 claims 1
- 210000001744 T-lymphocyte Anatomy 0.000 claims 1
- 210000003719 b-lymphocyte Anatomy 0.000 claims 1
- 210000004027 cell Anatomy 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 238000007865 diluting Methods 0.000 claims 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 claims 1
- 238000007857 nested PCR Methods 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 238000000926 separation method Methods 0.000 claims 1
- 239000000758 substrate Substances 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2005327174A JP4069133B2 (ja) | 2004-11-19 | 2005-11-11 | 生物試料に含まれる目的遺伝子を増幅する方法 |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2004336090 | 2004-11-19 | ||
| JP2005327174A JP4069133B2 (ja) | 2004-11-19 | 2005-11-11 | 生物試料に含まれる目的遺伝子を増幅する方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2006166907A JP2006166907A (ja) | 2006-06-29 |
| JP2006166907A5 true JP2006166907A5 (enExample) | 2007-03-22 |
| JP4069133B2 JP4069133B2 (ja) | 2008-04-02 |
Family
ID=36668269
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2005327174A Expired - Fee Related JP4069133B2 (ja) | 2004-11-19 | 2005-11-11 | 生物試料に含まれる目的遺伝子を増幅する方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP4069133B2 (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009098988A1 (ja) * | 2008-02-04 | 2009-08-13 | Genecare Research Institute Co., Ltd. | siRNA検出方法 |
| JP6164759B2 (ja) | 2013-11-21 | 2017-07-19 | Ｒｅｐｅｒｔｏｉｒｅ Ｇｅｎｅｓｉｓ株式会社 | Ｔ細胞受容体およびｂ細胞受容体レパトアの解析システムならびにその治療および診断への利用 |
| US10533204B2 (en) | 2014-05-30 | 2020-01-14 | National University Corporatlon University of Toyama | Method for amplifying a T cell receptor (TCR) cDNA |
| CN109517889B (zh) | 2017-09-18 | 2022-04-05 | 苏州吉赛基因测序科技有限公司 | 一种基于高通量测序分析寡核苷酸序列杂质的方法及应用 |
| ES3015628T3 (en) * | 2019-08-20 | 2025-05-06 | Director General Of Nat Institute Of Infectious Diseases | Method for amplifying nucleotide sequence, and sequence determination method |
| CN113774496A (zh) * | 2021-10-11 | 2021-12-10 | 湖南大地同年生物科技有限公司 | 一种液相捕获文库构建方法 |
-
2005
- 2005-11-11 JP JP2005327174A patent/JP4069133B2/ja not_active Expired - Fee Related
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