CA2670300C - Improved method for the prevention of carryover contamination in nucleic acid amplification technologies - Google Patents
Improved method for the prevention of carryover contamination in nucleic acid amplification technologies Download PDFInfo
- Publication number
- CA2670300C CA2670300C CA2670300A CA2670300A CA2670300C CA 2670300 C CA2670300 C CA 2670300C CA 2670300 A CA2670300 A CA 2670300A CA 2670300 A CA2670300 A CA 2670300A CA 2670300 C CA2670300 C CA 2670300C
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- Prior art keywords
- dna
- ung
- amplification
- abasic
- spermine
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US7611108P | 2008-06-26 | 2008-06-26 | |
| US61/076,111 | 2008-06-26 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2670300A1 CA2670300A1 (en) | 2009-12-26 |
| CA2670300C true CA2670300C (en) | 2014-12-09 |
Family
ID=41136785
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2670300A Expired - Fee Related CA2670300C (en) | 2008-06-26 | 2009-06-25 | Improved method for the prevention of carryover contamination in nucleic acid amplification technologies |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US8669061B2 (enExample) |
| EP (1) | EP2138590B1 (enExample) |
| JP (1) | JP5596308B2 (enExample) |
| CN (1) | CN101613730B (enExample) |
| CA (1) | CA2670300C (enExample) |
| ES (1) | ES2386023T3 (enExample) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8669061B2 (en) | 2008-06-26 | 2014-03-11 | Roche Molecular Systems, Inc. | Method for the prevention of carryover contamination in nucleic acid amplification technologies |
| WO2013022779A1 (en) * | 2011-08-05 | 2013-02-14 | Ibis Biosciences, Inc. | Analysis of genetic biomarkers for forensic analysis and fingerprinting |
| ES2449665B2 (es) * | 2012-09-19 | 2014-07-17 | Servizo Galego De Saúde-Conselleria De Sanidade, Xunta De Galicia | Procedimiento para el diagnóstico clínico de una enfermedad infecciosa basada en el empleo de la PCR cuantitativa, oligonucleótidos marcados de 8 a 9 nucleótidos de longitud y la UNG del Bacalao del Atlántico (Gadus morhua). |
| EP3071711A1 (en) * | 2013-11-18 | 2016-09-28 | Rubicon Genomics | Degradable adaptors for background reduction |
| US10036061B2 (en) * | 2013-12-20 | 2018-07-31 | Roche Molecular Systems, Inc. | Oligonucleotide inhibitor of DNA polymerases |
| AU2015270774B2 (en) * | 2014-06-05 | 2021-10-14 | Qiagen Gmbh | Optimization of DNA amplification reactions |
| AU2015330844B2 (en) | 2014-10-08 | 2022-02-03 | Cornell University | Method for identification and quantification of nucleic acid expression, splice variant, translocation, copy number, or methylation changes |
| CN104389026A (zh) * | 2014-10-30 | 2015-03-04 | 北京诺禾致源生物信息科技有限公司 | 单细胞转录组测序文库的构建方法及其应用 |
| JP6623324B2 (ja) * | 2015-09-07 | 2019-12-25 | 株式会社ファスマック | 等温増幅反応産物の多項目同時検出方法 |
| EP3485038B1 (en) | 2016-07-12 | 2020-12-23 | H. Hoffnabb-La Roche Ag | Primer extension target enrichment |
| WO2018015318A1 (en) | 2016-07-18 | 2018-01-25 | F. Hoffmann-La Roche Ag | Method for generating single-stranded circular dna libraries for single molecule sequencing |
| EP4269582A3 (en) | 2017-12-21 | 2024-01-24 | F. Hoffmann-La Roche AG | Target enrichment by unidirectional dual probe primer extension |
| CN119932089A (zh) * | 2019-05-07 | 2025-05-06 | 苏州齐禾生科生物科技有限公司 | 改进的基因编辑系统 |
| WO2021053008A1 (en) | 2019-09-20 | 2021-03-25 | F. Hoffmann-La Roche Ag | Immune repertoire profiling by primer extension target enrichment |
| CN115698337A (zh) | 2020-06-08 | 2023-02-03 | 豪夫迈·罗氏有限公司 | 用于检测基因组中的结构重排的方法和组合物 |
| CN113186037B (zh) * | 2021-04-23 | 2022-12-13 | 山东博弘基因科技有限公司 | 一种核酸清除剂 |
| US20250002900A1 (en) * | 2021-10-15 | 2025-01-02 | Qiagen Sciences, Llc | Methods for Producing DNA Libraries and Uses Thereof |
| CN114214395A (zh) * | 2021-12-31 | 2022-03-22 | 上海星耀医学科技发展有限公司 | 一种提高基因检测准确性的核酸扩增方法与试剂盒 |
| JP2025522981A (ja) | 2022-07-14 | 2025-07-17 | エフ． ホフマン－ラ ロシュ アーゲー | 一方向性デュアルプローブプライマー伸長によるバリアント対立遺伝子の濃縮 |
| CN116773291B (zh) * | 2023-03-31 | 2024-04-19 | 艾普拜生物科技(苏州)有限公司 | 一种石蜡切片dna预处理试剂及方法 |
| WO2024222446A1 (zh) * | 2023-04-23 | 2024-10-31 | 南京诺唯赞生物科技股份有限公司 | 一种去除非目标核酸模板的方法 |
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| JPH089997B2 (ja) | 1987-05-26 | 1996-01-31 | 日立冷熱株式会社 | 多翼送風機 |
| US5683896A (en) * | 1989-06-01 | 1997-11-04 | Life Technologies, Inc. | Process for controlling contamination of nucleic acid amplification reactions |
| DK0540693T3 (da) * | 1990-07-24 | 1999-09-13 | Hoffmann La Roche | Reduktion af ikke-specifik amplifikation under in vitro-nukleinsyreamplifikation under anvendelse af modificerede nukleinsy |
| CA2073298C (en) * | 1991-07-12 | 2007-04-17 | James L. Hartley | Process for controlling contamination of nucleic acid amplification reactions |
| JP3416981B2 (ja) | 1993-03-30 | 2003-06-16 | 株式会社島津製作所 | 核酸合成法 |
| JPH089997A (ja) | 1994-06-28 | 1996-01-16 | Shimadzu Corp | 核酸合成法およびそれに用いる試薬キット |
| US6413747B1 (en) * | 1994-10-03 | 2002-07-02 | Shimadzu Corporation | Enhancement of nucleic acid amplification by the addition of a polyamine |
| US6190865B1 (en) | 1995-09-27 | 2001-02-20 | Epicentre Technologies Corporation | Method for characterizing nucleic acid molecules |
| DE19545320A1 (de) | 1995-12-05 | 1997-06-12 | Boehringer Mannheim Gmbh | Thermolabile Uracil-DNA-Glykosylase, Verfahren zu deren Herstellung und Verwendung zur Entfernung von Uracil aus DNA |
| GB9600384D0 (en) | 1996-01-09 | 1996-03-13 | Nyfotek As | Dna glycosylases |
| JP4629167B2 (ja) | 1997-10-17 | 2011-02-09 | 株式会社島津製作所 | 核酸合成法 |
| EP1041159A3 (de) | 1999-03-26 | 2000-10-25 | MIRA Diagnostika GmbH | Verfahren, Oligonucleotide zum Abbau von in-vitro synthetisierten Nucleinsäuremolekülen |
| EP1038974A1 (de) | 1999-03-26 | 2000-09-27 | Mira Diagnostica GmbH | Verfahren, Oligonucleotide zum Abbau von in-vitro synthetisierten Nucleinsäuremolekülen |
| JP2001008680A (ja) | 1999-06-30 | 2001-01-16 | Shimadzu Corp | 核酸合成法 |
| US20040152094A1 (en) | 2001-05-10 | 2004-08-05 | Steffen Danielsen | Method for producing recombined polynucleotides |
| US20100022403A1 (en) * | 2006-06-30 | 2010-01-28 | Nurith Kurn | Methods for fragmentation and labeling of nucleic acids |
| US8669061B2 (en) | 2008-06-26 | 2014-03-11 | Roche Molecular Systems, Inc. | Method for the prevention of carryover contamination in nucleic acid amplification technologies |
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