JP5596308B2 - 核酸増幅技術におけるキャリーオーバー汚染の防止のための改良方法 - Google Patents

核酸増幅技術におけるキャリーオーバー汚染の防止のための改良方法 Download PDF

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JP5596308B2
JP5596308B2 JP2009151195A JP2009151195A JP5596308B2 JP 5596308 B2 JP5596308 B2 JP 5596308B2 JP 2009151195 A JP2009151195 A JP 2009151195A JP 2009151195 A JP2009151195 A JP 2009151195A JP 5596308 B2 JP5596308 B2 JP 5596308B2
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dna
amplification
reagent
nucleic acid
abasic
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JP2010004884A (ja
JP2010004884A5 (enExample
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ボヘンスキー ロイ
グプタ アマー
モンティール ジェニン
ゴードン ウィル スティーブン
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Immunology (AREA)
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  • Molecular Biology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2009151195A 2008-06-26 2009-06-25 核酸増幅技術におけるキャリーオーバー汚染の防止のための改良方法 Active JP5596308B2 (ja)

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US7611108P 2008-06-26 2008-06-26
US61/076,111 2008-06-26

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JP2010004884A JP2010004884A (ja) 2010-01-14
JP2010004884A5 JP2010004884A5 (enExample) 2012-03-08
JP5596308B2 true JP5596308B2 (ja) 2014-09-24

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US (1) US8669061B2 (enExample)
EP (1) EP2138590B1 (enExample)
JP (1) JP5596308B2 (enExample)
CN (1) CN101613730B (enExample)
CA (1) CA2670300C (enExample)
ES (1) ES2386023T3 (enExample)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8669061B2 (en) 2008-06-26 2014-03-11 Roche Molecular Systems, Inc. Method for the prevention of carryover contamination in nucleic acid amplification technologies
WO2013022779A1 (en) * 2011-08-05 2013-02-14 Ibis Biosciences, Inc. Analysis of genetic biomarkers for forensic analysis and fingerprinting
ES2449665B2 (es) * 2012-09-19 2014-07-17 Servizo Galego De Saúde-Conselleria De Sanidade, Xunta De Galicia Procedimiento para el diagnóstico clínico de una enfermedad infecciosa basada en el empleo de la PCR cuantitativa, oligonucleótidos marcados de 8 a 9 nucleótidos de longitud y la UNG del Bacalao del Atlántico (Gadus morhua).
JP2016537003A (ja) * 2013-11-18 2016-12-01 ルビコン ゲノミクス インコーポレイテッド バックグラウンド減少のための分解可能なアダプター
US10036061B2 (en) * 2013-12-20 2018-07-31 Roche Molecular Systems, Inc. Oligonucleotide inhibitor of DNA polymerases
JP6674905B2 (ja) * 2014-06-05 2020-04-01 キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング Ｄｎａ増幅反応の最適化
EP3690060B1 (en) 2014-10-08 2021-12-08 Cornell University Method for identification and relative quantification of nucleic acid sequence expression, splice variant, translocation, copy number, or methylation changes using combined nuclease, ligation, and polymerase reactions with carryover prevention
CN104389026A (zh) * 2014-10-30 2015-03-04 北京诺禾致源生物信息科技有限公司 单细胞转录组测序文库的构建方法及其应用
JP6623324B2 (ja) * 2015-09-07 2019-12-25 株式会社ファスマック 等温増幅反応産物の多項目同時検出方法
ES2855748T3 (es) 2016-07-12 2021-09-24 Hoffmann La Roche Enriquecimiento de diana de extensión de cebador
WO2018015318A1 (en) 2016-07-18 2018-01-25 F. Hoffmann-La Roche Ag Method for generating single-stranded circular dna libraries for single molecule sequencing
CN111757934A (zh) 2017-12-21 2020-10-09 豪夫迈·罗氏有限公司 通过单向双重探针引物延伸的靶标富集
AR123675A1 (es) * 2019-05-07 2023-01-04 Shanghai Bluecross Medical Science Inst Sistema mejorado de edición de genes
US20220372566A1 (en) 2019-09-20 2022-11-24 Roche Sequencing Solutions, Inc. Immune repertoire profiling by primer extension target enrichment
JP7633283B2 (ja) 2020-06-08 2025-02-19 エフ． ホフマン－ラ ロシュ アーゲー ゲノム内の構造再編成を検出するための方法及び組成物
CN113186037B (zh) * 2021-04-23 2022-12-13 山东博弘基因科技有限公司 一种核酸清除剂
CN118401675A (zh) * 2021-10-15 2024-07-26 凯杰科学有限责任公司 制备dna文库的方法及其用途
CN114214395A (zh) * 2021-12-31 2022-03-22 上海星耀医学科技发展有限公司 一种提高基因检测准确性的核酸扩增方法与试剂盒
WO2024013241A1 (en) 2022-07-14 2024-01-18 F. Hoffmann-La Roche Ag Variant allele enrichment by unidirectional dual probe primer extension
CN116773291B (zh) * 2023-03-31 2024-04-19 艾普拜生物科技(苏州)有限公司 一种石蜡切片dna预处理试剂及方法
WO2024222446A1 (zh) * 2023-04-23 2024-10-31 南京诺唯赞生物科技股份有限公司 一种去除非目标核酸模板的方法

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JPH089997B2 (ja) 1987-05-26 1996-01-31 日立冷熱株式会社 多翼送風機
US5683896A (en) 1989-06-01 1997-11-04 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
ATE176002T1 (de) * 1990-07-24 1999-02-15 Hoffmann La Roche Verringerung von nicht spezifischer amplifikation während einer (in vitro) nukleinsäure amplifikation unter verwendung von modifizierten nukleinsäure basen
CA2073298C (en) * 1991-07-12 2007-04-17 James L. Hartley Process for controlling contamination of nucleic acid amplification reactions
JP3416981B2 (ja) 1993-03-30 2003-06-16 株式会社島津製作所 核酸合成法
JPH089997A (ja) 1994-06-28 1996-01-16 Shimadzu Corp 核酸合成法およびそれに用いる試薬キット
US6413747B1 (en) 1994-10-03 2002-07-02 Shimadzu Corporation Enhancement of nucleic acid amplification by the addition of a polyamine
US6190865B1 (en) 1995-09-27 2001-02-20 Epicentre Technologies Corporation Method for characterizing nucleic acid molecules
DE19545320A1 (de) 1995-12-05 1997-06-12 Boehringer Mannheim Gmbh Thermolabile Uracil-DNA-Glykosylase, Verfahren zu deren Herstellung und Verwendung zur Entfernung von Uracil aus DNA
GB9600384D0 (en) 1996-01-09 1996-03-13 Nyfotek As Dna glycosylases
JP4629167B2 (ja) 1997-10-17 2011-02-09 株式会社島津製作所 核酸合成法
EP1038974A1 (de) 1999-03-26 2000-09-27 Mira Diagnostica GmbH Verfahren, Oligonucleotide zum Abbau von in-vitro synthetisierten Nucleinsäuremolekülen
EP1041159A3 (de) 1999-03-26 2000-10-25 MIRA Diagnostika GmbH Verfahren, Oligonucleotide zum Abbau von in-vitro synthetisierten Nucleinsäuremolekülen
JP2001008680A (ja) 1999-06-30 2001-01-16 Shimadzu Corp 核酸合成法
US20040152094A1 (en) 2001-05-10 2004-08-05 Steffen Danielsen Method for producing recombined polynucleotides
US20100022403A1 (en) * 2006-06-30 2010-01-28 Nurith Kurn Methods for fragmentation and labeling of nucleic acids
US8669061B2 (en) 2008-06-26 2014-03-11 Roche Molecular Systems, Inc. Method for the prevention of carryover contamination in nucleic acid amplification technologies

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CA2670300A1 (en) 2009-12-26
US8669061B2 (en) 2014-03-11
US20100093041A1 (en) 2010-04-15
JP2010004884A (ja) 2010-01-14
EP2138590B1 (en) 2012-05-30
CN101613730B (zh) 2014-06-04
CA2670300C (en) 2014-12-09
HK1138330A1 (en) 2010-08-20
ES2386023T3 (es) 2012-08-07
CN101613730A (zh) 2009-12-30
EP2138590A1 (en) 2009-12-30

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