JP2010500044A5 - - Google Patents
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- Publication number
- JP2010500044A5 JP2010500044A5 JP2009524183A JP2009524183A JP2010500044A5 JP 2010500044 A5 JP2010500044 A5 JP 2010500044A5 JP 2009524183 A JP2009524183 A JP 2009524183A JP 2009524183 A JP2009524183 A JP 2009524183A JP 2010500044 A5 JP2010500044 A5 JP 2010500044A5
- Authority
- JP
- Japan
- Prior art keywords
- enzyme
- triphosphate
- group
- poly
- reverse transcriptase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 25
- 102000004190 Enzymes Human genes 0.000 claims 16
- 108090000790 Enzymes Proteins 0.000 claims 16
- 230000000694 effects Effects 0.000 claims 9
- 239000005547 deoxyribonucleotide Substances 0.000 claims 8
- 125000002637 deoxyribonucleotide group Chemical group 0.000 claims 8
- 239000001226 triphosphate Substances 0.000 claims 8
- 102100034343 Integrase Human genes 0.000 claims 7
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims 7
- 108091034117 Oligonucleotide Proteins 0.000 claims 5
- 101710124239 Poly(A) polymerase Proteins 0.000 claims 5
- 238000006243 chemical reaction Methods 0.000 claims 5
- 108091028664 Ribonucleotide Proteins 0.000 claims 4
- 230000008488 polyadenylation Effects 0.000 claims 4
- 239000002336 ribonucleotide Substances 0.000 claims 4
- 125000002652 ribonucleotide group Chemical group 0.000 claims 4
- 239000000523 sample Substances 0.000 claims 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 3
- 239000000872 buffer Substances 0.000 claims 3
- 239000002299 complementary DNA Substances 0.000 claims 3
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 claims 3
- 239000011541 reaction mixture Substances 0.000 claims 3
- 108020004414 DNA Proteins 0.000 claims 2
- 102000053602 DNA Human genes 0.000 claims 2
- 108091093037 Peptide nucleic acid Proteins 0.000 claims 2
- 239000002253 acid Substances 0.000 claims 2
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 claims 2
- 229920001519 homopolymer Polymers 0.000 claims 2
- 238000003752 polymerase chain reaction Methods 0.000 claims 2
- 238000002360 preparation method Methods 0.000 claims 2
- 229920002477 rna polymer Polymers 0.000 claims 2
- ISIJQEHRDSCQIU-UHFFFAOYSA-N tert-butyl 2,7-diazaspiro[4.5]decane-7-carboxylate Chemical compound C1N(C(=O)OC(C)(C)C)CCCC11CNCC1 ISIJQEHRDSCQIU-UHFFFAOYSA-N 0.000 claims 2
- ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical group C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 claims 1
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 claims 1
- 241000203069 Archaea Species 0.000 claims 1
- 108020005174 Archaeal RNA Proteins 0.000 claims 1
- 241000894006 Bacteria Species 0.000 claims 1
- 241000283690 Bos taurus Species 0.000 claims 1
- 102000004594 DNA Polymerase I Human genes 0.000 claims 1
- 230000006820 DNA synthesis Effects 0.000 claims 1
- SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 claims 1
- 241000588724 Escherichia coli Species 0.000 claims 1
- 241000206602 Eukaryota Species 0.000 claims 1
- 108010078851 HIV Reverse Transcriptase Proteins 0.000 claims 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 1
- 108020003224 Small Nucleolar RNA Proteins 0.000 claims 1
- 102000042773 Small Nucleolar RNA Human genes 0.000 claims 1
- 108010085671 Thermus thermophilus DNA polymerase Proteins 0.000 claims 1
- 108020004566 Transfer RNA Proteins 0.000 claims 1
- 108020000999 Viral RNA Proteins 0.000 claims 1
- 241000700605 Viruses Species 0.000 claims 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 1
- 229960001456 adenosine triphosphate Drugs 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 230000006287 biotinylation Effects 0.000 claims 1
- 238000007413 biotinylation Methods 0.000 claims 1
- 239000007853 buffer solution Substances 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- -1 dCTP Chemical compound 0.000 claims 1
- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 claims 1
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 claims 1
- 238000006911 enzymatic reaction Methods 0.000 claims 1
- 239000007850 fluorescent dye Substances 0.000 claims 1
- 150000002500 ions Chemical class 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 108020004999 messenger RNA Proteins 0.000 claims 1
- 108091070501 miRNA Proteins 0.000 claims 1
- 239000002679 microRNA Substances 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 230000004048 modification Effects 0.000 claims 1
- 238000012986 modification Methods 0.000 claims 1
- 238000002703 mutagenesis Methods 0.000 claims 1
- 231100000350 mutagenesis Toxicity 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 239000002157 polynucleotide Substances 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 230000002285 radioactive effect Effects 0.000 claims 1
- 238000011895 specific detection Methods 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
- 230000003612 virological effect Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102006038113A DE102006038113A1 (de) | 2006-08-14 | 2006-08-14 | Verfahren zur Synthese einer cDNA in einer Probe in einer enzymatischen Reaktion und gleichzeitigen Bereitstellung eines ersten Enzyms mit Polyadenylierungsaktivität und eines zweiten Enzyms mit reverser Transkriptaseaktivität |
| DE102006038113.0 | 2006-08-14 | ||
| PCT/EP2007/058369 WO2008020008A1 (de) | 2006-08-14 | 2007-08-13 | VERFAHREN ZUR SYNTHESE EINER cDNA IN EINER PROBE IN EINER ENZYMATISCHEN REAKTION |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010500044A JP2010500044A (ja) | 2010-01-07 |
| JP2010500044A5 true JP2010500044A5 (enExample) | 2013-11-14 |
| JP5409360B2 JP5409360B2 (ja) | 2014-02-05 |
Family
ID=38669769
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009524183A Active JP5409360B2 (ja) | 2006-08-14 | 2007-08-13 | 酵素反応における試料中のｃＤＮＡの合成方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US9217173B2 (enExample) |
| EP (1) | EP2052086B1 (enExample) |
| JP (1) | JP5409360B2 (enExample) |
| CN (2) | CN101636505B (enExample) |
| AU (1) | AU2007286300B2 (enExample) |
| DE (1) | DE102006038113A1 (enExample) |
| WO (1) | WO2008020008A1 (enExample) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009021757A1 (de) * | 2007-08-13 | 2009-02-19 | Qiagen Gmbh | Verfahren zur synthese einer cdna in einer probe in einer enzymatischen reaktion |
| DE102006038113A1 (de) * | 2006-08-14 | 2008-02-21 | Qiagen Gmbh | Verfahren zur Synthese einer cDNA in einer Probe in einer enzymatischen Reaktion und gleichzeitigen Bereitstellung eines ersten Enzyms mit Polyadenylierungsaktivität und eines zweiten Enzyms mit reverser Transkriptaseaktivität |
| US8936909B2 (en) | 2008-07-18 | 2015-01-20 | Qiagen Gmbh | Method for determining the origin of a sample |
| DK2391736T3 (en) | 2009-02-02 | 2015-05-11 | Exiqon As | A method for the quantitation of small RNA species |
| EP2280081A1 (en) * | 2009-07-31 | 2011-02-02 | Qiagen GmbH | Method of normalized quantification of RNA |
| EP2280079A1 (en) * | 2009-07-31 | 2011-02-02 | Qiagen GmbH | Ligation-based method of normalized quantification of nucleic acids |
| EP2280080A1 (en) | 2009-07-31 | 2011-02-02 | Qiagen GmbH | Method of normalized quantification of nucleic acids using anchor oligonucleotides and adapter oligonucleotides |
| KR101818126B1 (ko) * | 2011-02-09 | 2018-01-15 | (주)바이오니아 | 열안정성이 증가된 역전사효소 |
| WO2013111800A1 (ja) | 2012-01-25 | 2013-08-01 | 独立行政法人科学技術振興機構 | Ｈｉｖ検出用オリゴヌクレオチド、ｈｉｖ検出キット、及びｈｉｖ検出方法 |
| JP6338221B2 (ja) | 2012-10-24 | 2018-06-06 | タカラ バイオ ユーエスエー， インコーポレイテッド | 核酸生成物を生成するための、テンプレートスイッチに基づく方法 |
| DK2914741T3 (da) | 2012-11-02 | 2017-11-20 | Life Technologies Corp | Hidtil ukendte sammensætninger og fremgangsmåder til forbedring af PCR-specificitet |
| JP6602294B2 (ja) | 2013-10-17 | 2019-11-06 | タカラ バイオ ユーエスエー， インコーポレイテッド | アダプタを核酸に追加する方法、及びこの方法を実行するための組成物 |
| WO2015094861A1 (en) | 2013-12-17 | 2015-06-25 | Clontech Laboratories, Inc. | Methods for adding adapters to nucleic acids and compositions for practicing the same |
| WO2016092062A1 (en) * | 2014-12-11 | 2016-06-16 | Qiagen Gmbh | Method for improving the efficiency of cdna generation |
| EP3967768A1 (en) | 2015-03-13 | 2022-03-16 | Life Technologies Corporation | Compositions for small rna capture, detection and quantification |
| CN107541507A (zh) * | 2016-06-29 | 2018-01-05 | 厦门大学 | 一种rna反转录扩增方法 |
| AU2018272302A1 (en) * | 2017-05-26 | 2019-12-19 | Nuclera Nucleics Ltd | Use of terminal transferase enzyme in nucleic acid synthesis |
| US20220298555A1 (en) * | 2018-12-20 | 2022-09-22 | Roche Molecular Systems, Inc. | Detection of target nucleic acid by solid-phase molography |
| CN113924492B (zh) * | 2019-05-06 | 2024-07-16 | 宝洁公司 | 使用适配体检测口腔样品中的微生物内毒素 |
| CA3178255A1 (en) * | 2020-05-15 | 2021-11-18 | Krishna KANNAN | On demand synthesis of polynucleotide sequences |
| US20220056510A1 (en) * | 2020-08-20 | 2022-02-24 | Detect, Inc. | Enzymatic tablet and uses thereof |
| EP4301499A4 (en) | 2021-03-05 | 2025-01-22 | Enumerix, Inc. | SYSTEMS AND METHODS FOR GENERATING DROPLETS AND PERFORMING DIGITAL ANALYSES |
| WO2023025259A1 (zh) * | 2021-08-25 | 2023-03-02 | 卓越精准医疗有限公司 | 检测微小rna的方法和试剂盒 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999043650A2 (en) | 1998-02-25 | 1999-09-02 | Smithkline Beecham Plc | Process(es) for the preparation of 6-trifluoromethyl-indoline derivatives |
| KR100440044B1 (ko) | 1998-02-27 | 2004-07-14 | 팸진 비.브이. | 핵산의 비특이적 증폭방법 |
| US6300069B1 (en) * | 1999-05-03 | 2001-10-09 | Qiagen Gmbh | Generation and amplification of nucleic acids from ribonucleic acids |
| US7141372B2 (en) * | 2002-01-18 | 2006-11-28 | Health Research Incorporated | Universal RT-coupled PCR method for the specific amplification of mRNA |
| ATE319861T1 (de) | 2002-10-30 | 2006-03-15 | Pamgene Bv | Verbesserte methoden zur erzeugung einer vielzahl von rna-kopien |
| AU2004309396B2 (en) | 2003-12-23 | 2010-05-13 | Genomic Health, Inc. | Universal amplification of fragmented RNA |
| CN1763223B (zh) * | 2004-10-19 | 2011-04-27 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | miRNA检测方法 |
| DE102006038113A1 (de) * | 2006-08-14 | 2008-02-21 | Qiagen Gmbh | Verfahren zur Synthese einer cDNA in einer Probe in einer enzymatischen Reaktion und gleichzeitigen Bereitstellung eines ersten Enzyms mit Polyadenylierungsaktivität und eines zweiten Enzyms mit reverser Transkriptaseaktivität |
| US8314220B2 (en) * | 2007-01-26 | 2012-11-20 | Agilent Technologies, Inc. | Methods compositions, and kits for detection of microRNA |
-
2006
- 2006-08-14 DE DE102006038113A patent/DE102006038113A1/de not_active Withdrawn
-
2007
- 2007-08-13 EP EP07788393A patent/EP2052086B1/de active Active
- 2007-08-13 JP JP2009524183A patent/JP5409360B2/ja active Active
- 2007-08-13 WO PCT/EP2007/058369 patent/WO2008020008A1/de not_active Ceased
- 2007-08-13 AU AU2007286300A patent/AU2007286300B2/en active Active
- 2007-08-13 CN CN2007800303214A patent/CN101636505B/zh active Active
- 2007-08-13 US US12/377,457 patent/US9217173B2/en active Active
-
2008
- 2008-02-13 CN CN2008801034578A patent/CN101802222B/zh active Active
- 2008-02-13 US US12/673,251 patent/US20110124050A1/en not_active Abandoned
-
2015
- 2015-11-12 US US14/939,153 patent/US9611506B2/en active Active
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