AU2007286300B2 - Methods for synthesis of a cDNA in a sample in an enzymatic reaction - Google Patents

Methods for synthesis of a cDNA in a sample in an enzymatic reaction Download PDF

Info

Publication number
AU2007286300B2
AU2007286300B2 AU2007286300A AU2007286300A AU2007286300B2 AU 2007286300 B2 AU2007286300 B2 AU 2007286300B2 AU 2007286300 A AU2007286300 A AU 2007286300A AU 2007286300 A AU2007286300 A AU 2007286300A AU 2007286300 B2 AU2007286300 B2 AU 2007286300B2
Authority
AU
Australia
Prior art keywords
reaction
poly
reverse transcriptase
optionally
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2007286300A
Other languages
English (en)
Other versions
AU2007286300A1 (en
Inventor
Holger Engel
Christian Korfhage
Martin Kreutz
Dirk Loffert
Subrahmanyam Yerramilli
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen GmbH
Original Assignee
Qiagen GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen GmbH filed Critical Qiagen GmbH
Publication of AU2007286300A1 publication Critical patent/AU2007286300A1/en
Application granted granted Critical
Publication of AU2007286300B2 publication Critical patent/AU2007286300B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2007286300A 2006-08-14 2007-08-13 Methods for synthesis of a cDNA in a sample in an enzymatic reaction Active AU2007286300B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102006038113A DE102006038113A1 (de) 2006-08-14 2006-08-14 Verfahren zur Synthese einer cDNA in einer Probe in einer enzymatischen Reaktion und gleichzeitigen Bereitstellung eines ersten Enzyms mit Polyadenylierungsaktivität und eines zweiten Enzyms mit reverser Transkriptaseaktivität
DE102006038113.0 2006-08-14
PCT/EP2007/058369 WO2008020008A1 (de) 2006-08-14 2007-08-13 VERFAHREN ZUR SYNTHESE EINER cDNA IN EINER PROBE IN EINER ENZYMATISCHEN REAKTION

Publications (2)

Publication Number Publication Date
AU2007286300A1 AU2007286300A1 (en) 2008-02-21
AU2007286300B2 true AU2007286300B2 (en) 2012-11-22

Family

ID=38669769

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2007286300A Active AU2007286300B2 (en) 2006-08-14 2007-08-13 Methods for synthesis of a cDNA in a sample in an enzymatic reaction

Country Status (7)

Country Link
US (3) US9217173B2 (enExample)
EP (1) EP2052086B1 (enExample)
JP (1) JP5409360B2 (enExample)
CN (2) CN101636505B (enExample)
AU (1) AU2007286300B2 (enExample)
DE (1) DE102006038113A1 (enExample)
WO (1) WO2008020008A1 (enExample)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009021757A1 (de) * 2007-08-13 2009-02-19 Qiagen Gmbh Verfahren zur synthese einer cdna in einer probe in einer enzymatischen reaktion
DE102006038113A1 (de) * 2006-08-14 2008-02-21 Qiagen Gmbh Verfahren zur Synthese einer cDNA in einer Probe in einer enzymatischen Reaktion und gleichzeitigen Bereitstellung eines ersten Enzyms mit Polyadenylierungsaktivität und eines zweiten Enzyms mit reverser Transkriptaseaktivität
US8936909B2 (en) 2008-07-18 2015-01-20 Qiagen Gmbh Method for determining the origin of a sample
DK2391736T3 (en) 2009-02-02 2015-05-11 Exiqon As A method for the quantitation of small RNA species
EP2280081A1 (en) * 2009-07-31 2011-02-02 Qiagen GmbH Method of normalized quantification of RNA
EP2280079A1 (en) * 2009-07-31 2011-02-02 Qiagen GmbH Ligation-based method of normalized quantification of nucleic acids
EP2280080A1 (en) 2009-07-31 2011-02-02 Qiagen GmbH Method of normalized quantification of nucleic acids using anchor oligonucleotides and adapter oligonucleotides
KR101818126B1 (ko) * 2011-02-09 2018-01-15 (주)바이오니아 열안정성이 증가된 역전사효소
WO2013111800A1 (ja) 2012-01-25 2013-08-01 独立行政法人科学技術振興機構 Ｈｉｖ検出用オリゴヌクレオチド、ｈｉｖ検出キット、及びｈｉｖ検出方法
JP6338221B2 (ja) 2012-10-24 2018-06-06 タカラ バイオ ユーエスエー， インコーポレイテッド 核酸生成物を生成するための、テンプレートスイッチに基づく方法
DK2914741T3 (da) 2012-11-02 2017-11-20 Life Technologies Corp Hidtil ukendte sammensætninger og fremgangsmåder til forbedring af PCR-specificitet
JP6602294B2 (ja) 2013-10-17 2019-11-06 タカラ バイオ ユーエスエー， インコーポレイテッド アダプタを核酸に追加する方法、及びこの方法を実行するための組成物
WO2015094861A1 (en) 2013-12-17 2015-06-25 Clontech Laboratories, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
WO2016092062A1 (en) * 2014-12-11 2016-06-16 Qiagen Gmbh Method for improving the efficiency of cdna generation
EP3967768A1 (en) 2015-03-13 2022-03-16 Life Technologies Corporation Compositions for small rna capture, detection and quantification
CN107541507A (zh) * 2016-06-29 2018-01-05 厦门大学 一种rna反转录扩增方法
AU2018272302A1 (en) * 2017-05-26 2019-12-19 Nuclera Nucleics Ltd Use of terminal transferase enzyme in nucleic acid synthesis
US20220298555A1 (en) * 2018-12-20 2022-09-22 Roche Molecular Systems, Inc. Detection of target nucleic acid by solid-phase molography
CN113924492B (zh) * 2019-05-06 2024-07-16 宝洁公司 使用适配体检测口腔样品中的微生物内毒素
CA3178255A1 (en) * 2020-05-15 2021-11-18 Krishna KANNAN On demand synthesis of polynucleotide sequences
US20220056510A1 (en) * 2020-08-20 2022-02-24 Detect, Inc. Enzymatic tablet and uses thereof
EP4301499A4 (en) 2021-03-05 2025-01-22 Enumerix, Inc. SYSTEMS AND METHODS FOR GENERATING DROPLETS AND PERFORMING DIGITAL ANALYSES
WO2023025259A1 (zh) * 2021-08-25 2023-03-02 卓越精准医疗有限公司 检测微小rna的方法和试剂盒

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030113875A1 (en) * 1998-02-27 2003-06-19 Gemen Bob Van Method for the non-specific amplification of nucleic acid
WO2004044239A1 (en) * 2002-10-30 2004-05-27 Pamgene B.V. Improved methods for generating multiple rna copies

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999043650A2 (en) 1998-02-25 1999-09-02 Smithkline Beecham Plc Process(es) for the preparation of 6-trifluoromethyl-indoline derivatives
US6300069B1 (en) * 1999-05-03 2001-10-09 Qiagen Gmbh Generation and amplification of nucleic acids from ribonucleic acids
US7141372B2 (en) * 2002-01-18 2006-11-28 Health Research Incorporated Universal RT-coupled PCR method for the specific amplification of mRNA
AU2004309396B2 (en) 2003-12-23 2010-05-13 Genomic Health, Inc. Universal amplification of fragmented RNA
CN1763223B (zh) * 2004-10-19 2011-04-27 中国人民解放军军事医学科学院放射与辐射医学研究所 miRNA检测方法
DE102006038113A1 (de) * 2006-08-14 2008-02-21 Qiagen Gmbh Verfahren zur Synthese einer cDNA in einer Probe in einer enzymatischen Reaktion und gleichzeitigen Bereitstellung eines ersten Enzyms mit Polyadenylierungsaktivität und eines zweiten Enzyms mit reverser Transkriptaseaktivität
US8314220B2 (en) * 2007-01-26 2012-11-20 Agilent Technologies, Inc. Methods compositions, and kits for detection of microRNA

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030113875A1 (en) * 1998-02-27 2003-06-19 Gemen Bob Van Method for the non-specific amplification of nucleic acid
WO2004044239A1 (en) * 2002-10-30 2004-05-27 Pamgene B.V. Improved methods for generating multiple rna copies

Also Published As

Publication number Publication date
US9217173B2 (en) 2015-12-22
CN101636505A (zh) 2010-01-27
DE102006038113A1 (de) 2008-02-21
WO2008020008A1 (de) 2008-02-21
CN101802222A (zh) 2010-08-11
US20160130634A1 (en) 2016-05-12
AU2007286300A1 (en) 2008-02-21
CN101636505B (zh) 2012-09-05
EP2052086B1 (de) 2013-03-13
CN101802222B (zh) 2013-07-03
US20120202198A1 (en) 2012-08-09
US20110124050A1 (en) 2011-05-26
JP2010500044A (ja) 2010-01-07
US9611506B2 (en) 2017-04-04
EP2052086A1 (de) 2009-04-29
JP5409360B2 (ja) 2014-02-05

Similar Documents

Publication Publication Date Title
AU2007286300B2 (en) Methods for synthesis of a cDNA in a sample in an enzymatic reaction
EP1812599B1 (en) Methods and compositions for analysing ribonucleic acids
US8486628B2 (en) Method of normalized quantification of nucleic acids using anchor oligonucleotides and adapter oligonucleotides
JP5680078B2 (ja) ライゲーションに基づく核酸の正規化した定量化方法
WO2008097957A2 (en) Detection of mature small rna molecules
JP5680079B2 (ja) Ｒｎａの正規化した定量化方法
AU2008286254B2 (en) Method for synthesizing a cDNA in a sample in an enzymatic reaction
EP1275734A1 (en) Method for random cDNA synthesis and amplification
JP2010535525A5 (enExample)
US9587267B2 (en) Quantification of nucleic acids
EP2191008B1 (de) Verfahren zur synthese einer cdna in einer probe in einer enzymatischen reaktion
WO2016092062A1 (en) Method for improving the efficiency of cdna generation

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)