AU2007286300B2 - Methods for synthesis of a cDNA in a sample in an enzymatic reaction - Google Patents

Methods for synthesis of a cDNA in a sample in an enzymatic reaction Download PDF

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Publication number
AU2007286300B2
AU2007286300B2 AU2007286300A AU2007286300A AU2007286300B2 AU 2007286300 B2 AU2007286300 B2 AU 2007286300B2 AU 2007286300 A AU2007286300 A AU 2007286300A AU 2007286300 A AU2007286300 A AU 2007286300A AU 2007286300 B2 AU2007286300 B2 AU 2007286300B2
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Australia
Prior art keywords
reaction
poly
reverse transcriptase
optionally
enzyme
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AU2007286300A
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English (en)
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AU2007286300A1 (en
Inventor
Holger Engel
Christian Korfhage
Martin Kreutz
Dirk Loffert
Subrahmanyam Yerramilli
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Qiagen GmbH
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Qiagen GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2007286300A 2006-08-14 2007-08-13 Methods for synthesis of a cDNA in a sample in an enzymatic reaction Active AU2007286300B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102006038113.0 2006-08-14
DE102006038113A DE102006038113A1 (de) 2006-08-14 2006-08-14 Verfahren zur Synthese einer cDNA in einer Probe in einer enzymatischen Reaktion und gleichzeitigen Bereitstellung eines ersten Enzyms mit Polyadenylierungsaktivität und eines zweiten Enzyms mit reverser Transkriptaseaktivität
PCT/EP2007/058369 WO2008020008A1 (de) 2006-08-14 2007-08-13 VERFAHREN ZUR SYNTHESE EINER cDNA IN EINER PROBE IN EINER ENZYMATISCHEN REAKTION

Publications (2)

Publication Number Publication Date
AU2007286300A1 AU2007286300A1 (en) 2008-02-21
AU2007286300B2 true AU2007286300B2 (en) 2012-11-22

Family

ID=38669769

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2007286300A Active AU2007286300B2 (en) 2006-08-14 2007-08-13 Methods for synthesis of a cDNA in a sample in an enzymatic reaction

Country Status (7)

Country Link
US (3) US9217173B2 (enExample)
EP (1) EP2052086B1 (enExample)
JP (1) JP5409360B2 (enExample)
CN (2) CN101636505B (enExample)
AU (1) AU2007286300B2 (enExample)
DE (1) DE102006038113A1 (enExample)
WO (1) WO2008020008A1 (enExample)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006038113A1 (de) * 2006-08-14 2008-02-21 Qiagen Gmbh Verfahren zur Synthese einer cDNA in einer Probe in einer enzymatischen Reaktion und gleichzeitigen Bereitstellung eines ersten Enzyms mit Polyadenylierungsaktivität und eines zweiten Enzyms mit reverser Transkriptaseaktivität
AU2008286254B2 (en) * 2007-08-13 2014-01-30 Qiagen Gmbh Method for synthesizing a cDNA in a sample in an enzymatic reaction
US8936909B2 (en) 2008-07-18 2015-01-20 Qiagen Gmbh Method for determining the origin of a sample
CA2750029C (en) * 2009-02-02 2019-05-21 Exiqon A/S Method for quantification of small rna species
EP2280080A1 (en) * 2009-07-31 2011-02-02 Qiagen GmbH Method of normalized quantification of nucleic acids using anchor oligonucleotides and adapter oligonucleotides
EP2280081A1 (en) * 2009-07-31 2011-02-02 Qiagen GmbH Method of normalized quantification of RNA
EP2280079A1 (en) * 2009-07-31 2011-02-02 Qiagen GmbH Ligation-based method of normalized quantification of nucleic acids
KR101818126B1 (ko) 2011-02-09 2018-01-15 (주)바이오니아 열안정성이 증가된 역전사효소
JP5553323B2 (ja) 2012-01-25 2014-07-16 独立行政法人科学技術振興機構 Ｈｉｖ検出キット、及びｈｉｖ検出方法
WO2014066179A1 (en) 2012-10-24 2014-05-01 Clontech Laboratories, Inc. Template switch-based methods for producing a product nucleic acid
CN111793621B (zh) 2012-11-02 2024-12-10 生命技术公司 用于增强pcr特异性的新颖组合物、方法和试剂盒
US10941397B2 (en) 2013-10-17 2021-03-09 Takara Bio Usa, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
US9719136B2 (en) 2013-12-17 2017-08-01 Takara Bio Usa, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
WO2016092062A1 (en) * 2014-12-11 2016-06-16 Qiagen Gmbh Method for improving the efficiency of cdna generation
WO2016149021A1 (en) 2015-03-13 2016-09-22 Life Technologies Corporation Methods, compositions and kits for small rna capture, detection and quantification
CN107541507A (zh) * 2016-06-29 2018-01-05 厦门大学 一种rna反转录扩增方法
CN110997907A (zh) * 2017-05-26 2020-04-10 核酸有限公司 末端转移酶在核酸合成中的用途
CN113195734B (zh) * 2018-12-20 2024-12-24 豪夫迈·罗氏有限公司 通过固相分子成像检测靶核酸
US11643661B2 (en) * 2019-05-06 2023-05-09 The Procter & Gamble Company Detection of microbial endotoxins in oral samples using aptamers
US20220056510A1 (en) * 2020-08-20 2022-02-24 Detect, Inc. Enzymatic tablet and uses thereof
CN117529560A (zh) * 2021-08-25 2024-02-06 卓越精准医疗有限公司 检测微小rna的方法和试剂盒

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030113875A1 (en) * 1998-02-27 2003-06-19 Gemen Bob Van Method for the non-specific amplification of nucleic acid
WO2004044239A1 (en) * 2002-10-30 2004-05-27 Pamgene B.V. Improved methods for generating multiple rna copies

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999043650A2 (en) 1998-02-25 1999-09-02 Smithkline Beecham Plc Process(es) for the preparation of 6-trifluoromethyl-indoline derivatives
US6300069B1 (en) * 1999-05-03 2001-10-09 Qiagen Gmbh Generation and amplification of nucleic acids from ribonucleic acids
US7141372B2 (en) * 2002-01-18 2006-11-28 Health Research Incorporated Universal RT-coupled PCR method for the specific amplification of mRNA
US20050196782A1 (en) * 2003-12-23 2005-09-08 Kiefer Michael C. Universal amplification of fragmented RNA
CN1763223B (zh) * 2004-10-19 2011-04-27 中国人民解放军军事医学科学院放射与辐射医学研究所 miRNA检测方法
DE102006038113A1 (de) * 2006-08-14 2008-02-21 Qiagen Gmbh Verfahren zur Synthese einer cDNA in einer Probe in einer enzymatischen Reaktion und gleichzeitigen Bereitstellung eines ersten Enzyms mit Polyadenylierungsaktivität und eines zweiten Enzyms mit reverser Transkriptaseaktivität
US8314220B2 (en) * 2007-01-26 2012-11-20 Agilent Technologies, Inc. Methods compositions, and kits for detection of microRNA

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030113875A1 (en) * 1998-02-27 2003-06-19 Gemen Bob Van Method for the non-specific amplification of nucleic acid
WO2004044239A1 (en) * 2002-10-30 2004-05-27 Pamgene B.V. Improved methods for generating multiple rna copies

Also Published As

Publication number Publication date
CN101636505B (zh) 2012-09-05
US20160130634A1 (en) 2016-05-12
CN101802222A (zh) 2010-08-11
EP2052086B1 (de) 2013-03-13
WO2008020008A1 (de) 2008-02-21
US20110124050A1 (en) 2011-05-26
US9611506B2 (en) 2017-04-04
EP2052086A1 (de) 2009-04-29
DE102006038113A1 (de) 2008-02-21
JP2010500044A (ja) 2010-01-07
US9217173B2 (en) 2015-12-22
AU2007286300A1 (en) 2008-02-21
CN101802222B (zh) 2013-07-03
JP5409360B2 (ja) 2014-02-05
US20120202198A1 (en) 2012-08-09
CN101636505A (zh) 2010-01-27

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