AU2007286300B2 - Methods for synthesis of a cDNA in a sample in an enzymatic reaction - Google Patents

Methods for synthesis of a cDNA in a sample in an enzymatic reaction Download PDF

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Publication number
AU2007286300B2
AU2007286300B2 AU2007286300A AU2007286300A AU2007286300B2 AU 2007286300 B2 AU2007286300 B2 AU 2007286300B2 AU 2007286300 A AU2007286300 A AU 2007286300A AU 2007286300 A AU2007286300 A AU 2007286300A AU 2007286300 B2 AU2007286300 B2 AU 2007286300B2
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Australia
Prior art keywords
reaction
poly
reverse transcriptase
optionally
enzyme
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AU2007286300A
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English (en)
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AU2007286300A1 (en
Inventor
Holger Engel
Christian Korfhage
Martin Kreutz
Dirk Loffert
Subrahmanyam Yerramilli
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Qiagen GmbH
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Qiagen GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2007286300A 2006-08-14 2007-08-13 Methods for synthesis of a cDNA in a sample in an enzymatic reaction Active AU2007286300B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102006038113.0 2006-08-14
DE102006038113A DE102006038113A1 (de) 2006-08-14 2006-08-14 Verfahren zur Synthese einer cDNA in einer Probe in einer enzymatischen Reaktion und gleichzeitigen Bereitstellung eines ersten Enzyms mit Polyadenylierungsaktivität und eines zweiten Enzyms mit reverser Transkriptaseaktivität
PCT/EP2007/058369 WO2008020008A1 (de) 2006-08-14 2007-08-13 VERFAHREN ZUR SYNTHESE EINER cDNA IN EINER PROBE IN EINER ENZYMATISCHEN REAKTION

Publications (2)

Publication Number Publication Date
AU2007286300A1 AU2007286300A1 (en) 2008-02-21
AU2007286300B2 true AU2007286300B2 (en) 2012-11-22

Family

ID=38669769

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2007286300A Active AU2007286300B2 (en) 2006-08-14 2007-08-13 Methods for synthesis of a cDNA in a sample in an enzymatic reaction

Country Status (7)

Country Link
US (3) US9217173B2 (enExample)
EP (1) EP2052086B1 (enExample)
JP (1) JP5409360B2 (enExample)
CN (2) CN101636505B (enExample)
AU (1) AU2007286300B2 (enExample)
DE (1) DE102006038113A1 (enExample)
WO (1) WO2008020008A1 (enExample)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006038113A1 (de) * 2006-08-14 2008-02-21 Qiagen Gmbh Verfahren zur Synthese einer cDNA in einer Probe in einer enzymatischen Reaktion und gleichzeitigen Bereitstellung eines ersten Enzyms mit Polyadenylierungsaktivität und eines zweiten Enzyms mit reverser Transkriptaseaktivität
AU2008286254B2 (en) * 2007-08-13 2014-01-30 Qiagen Gmbh Method for synthesizing a cDNA in a sample in an enzymatic reaction
WO2010006814A1 (en) 2008-07-18 2010-01-21 Qiagen Gmbh Method for determining the origin of a sample
JP5749656B2 (ja) * 2009-02-02 2015-07-15 エクシコン エ／エス 低分子ｒｎａ種の定量化のための方法
EP2280081A1 (en) * 2009-07-31 2011-02-02 Qiagen GmbH Method of normalized quantification of RNA
EP2280080A1 (en) * 2009-07-31 2011-02-02 Qiagen GmbH Method of normalized quantification of nucleic acids using anchor oligonucleotides and adapter oligonucleotides
EP2280079A1 (en) * 2009-07-31 2011-02-02 Qiagen GmbH Ligation-based method of normalized quantification of nucleic acids
KR101818126B1 (ko) 2011-02-09 2018-01-15 (주)바이오니아 열안정성이 증가된 역전사효소
CN104066842A (zh) 2012-01-25 2014-09-24 独立行政法人科学技术振兴机构 Hiv检测用寡核苷酸、hiv检测试剂盒及hiv检测方法
US9410173B2 (en) 2012-10-24 2016-08-09 Clontech Laboratories, Inc. Template switch-based methods for producing a product nucleic acid
WO2014071322A1 (en) 2012-11-02 2014-05-08 Life Technologies Corporation Small RNA Capture, Detection and Quantification
EP3760733A1 (en) 2013-10-17 2021-01-06 Takara Bio USA, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
WO2015094861A1 (en) 2013-12-17 2015-06-25 Clontech Laboratories, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
WO2016092062A1 (en) * 2014-12-11 2016-06-16 Qiagen Gmbh Method for improving the efficiency of cdna generation
ES2890776T3 (es) 2015-03-13 2022-01-24 Life Technologies Corp Métodos, composiciones y kits para la captura, la detección y la cuantificación del ARN pequeño
CN107541507A (zh) * 2016-06-29 2018-01-05 厦门大学 一种rna反转录扩增方法
CA3064820A1 (en) * 2017-05-26 2018-11-29 Nuclera Nucleics Ltd Use of terminal transferase enzyme in nucleic acid synthesis
JP7249418B2 (ja) * 2018-12-20 2023-03-30 エフ． ホフマン－ラ ロシュ アーゲー 固相モログラフィーによる標的核酸の検出
CN113924492B (zh) * 2019-05-06 2024-07-16 宝洁公司 使用适配体检测口腔样品中的微生物内毒素
US20220056510A1 (en) * 2020-08-20 2022-02-24 Detect, Inc. Enzymatic tablet and uses thereof
EP4301499A4 (en) 2021-03-05 2025-01-22 Enumerix, Inc. SYSTEMS AND METHODS FOR GENERATING DROPLETS AND PERFORMING DIGITAL ANALYSES
CN117529560A (zh) * 2021-08-25 2024-02-06 卓越精准医疗有限公司 检测微小rna的方法和试剂盒

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030113875A1 (en) * 1998-02-27 2003-06-19 Gemen Bob Van Method for the non-specific amplification of nucleic acid
WO2004044239A1 (en) * 2002-10-30 2004-05-27 Pamgene B.V. Improved methods for generating multiple rna copies

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU3030899A (en) 1998-02-25 1999-09-15 Smithkline Beecham Plc Process(es) for the preparation of 6-trifluoromethyl-indoline derivatives
US6300069B1 (en) * 1999-05-03 2001-10-09 Qiagen Gmbh Generation and amplification of nucleic acids from ribonucleic acids
US7141372B2 (en) * 2002-01-18 2006-11-28 Health Research Incorporated Universal RT-coupled PCR method for the specific amplification of mRNA
ES2360113T3 (es) * 2003-12-23 2011-06-01 Genomic Health, Inc. Amplificación universal de rna fragmentado.
CN1763223B (zh) * 2004-10-19 2011-04-27 中国人民解放军军事医学科学院放射与辐射医学研究所 miRNA检测方法
DE102006038113A1 (de) * 2006-08-14 2008-02-21 Qiagen Gmbh Verfahren zur Synthese einer cDNA in einer Probe in einer enzymatischen Reaktion und gleichzeitigen Bereitstellung eines ersten Enzyms mit Polyadenylierungsaktivität und eines zweiten Enzyms mit reverser Transkriptaseaktivität
US8314220B2 (en) * 2007-01-26 2012-11-20 Agilent Technologies, Inc. Methods compositions, and kits for detection of microRNA

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030113875A1 (en) * 1998-02-27 2003-06-19 Gemen Bob Van Method for the non-specific amplification of nucleic acid
WO2004044239A1 (en) * 2002-10-30 2004-05-27 Pamgene B.V. Improved methods for generating multiple rna copies

Also Published As

Publication number Publication date
JP2010500044A (ja) 2010-01-07
EP2052086B1 (de) 2013-03-13
EP2052086A1 (de) 2009-04-29
CN101636505B (zh) 2012-09-05
WO2008020008A1 (de) 2008-02-21
US20160130634A1 (en) 2016-05-12
US20110124050A1 (en) 2011-05-26
CN101802222B (zh) 2013-07-03
US20120202198A1 (en) 2012-08-09
CN101802222A (zh) 2010-08-11
JP5409360B2 (ja) 2014-02-05
US9611506B2 (en) 2017-04-04
AU2007286300A1 (en) 2008-02-21
DE102006038113A1 (de) 2008-02-21
CN101636505A (zh) 2010-01-27
US9217173B2 (en) 2015-12-22

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