DE602006011917D1 - Verfahren und substanzen zur isolierung von mirnas - Google Patents
Verfahren und substanzen zur isolierung von mirnasInfo
- Publication number
- DE602006011917D1 DE602006011917D1 DE602006011917T DE602006011917T DE602006011917D1 DE 602006011917 D1 DE602006011917 D1 DE 602006011917D1 DE 602006011917 T DE602006011917 T DE 602006011917T DE 602006011917 T DE602006011917 T DE 602006011917T DE 602006011917 D1 DE602006011917 D1 DE 602006011917D1
- Authority
- DE
- Germany
- Prior art keywords
- substances
- isolating
- mirnas
- isolating mirnas
- mirna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US70986105P | 2005-08-19 | 2005-08-19 | |
PCT/US2006/032264 WO2007024653A2 (en) | 2005-08-19 | 2006-08-18 | Method and substances for isolating micro rnas |
Publications (1)
Publication Number | Publication Date |
---|---|
DE602006011917D1 true DE602006011917D1 (de) | 2010-03-11 |
Family
ID=37772191
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE602006011917T Active DE602006011917D1 (de) | 2005-08-19 | 2006-08-18 | Verfahren und substanzen zur isolierung von mirnas |
Country Status (7)
Country | Link |
---|---|
US (4) | US8278035B2 (de) |
EP (1) | EP1922420B1 (de) |
AT (1) | ATE455871T1 (de) |
CA (1) | CA2613136C (de) |
DE (1) | DE602006011917D1 (de) |
IL (1) | IL188599A (de) |
WO (1) | WO2007024653A2 (de) |
Families Citing this family (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8278035B2 (en) * | 2005-08-19 | 2012-10-02 | Bioventures, Inc. | Method and substances for isolating miRNAs |
CN101528763B (zh) | 2006-08-30 | 2012-06-27 | 生物风险公司 | 分离和检测小多核苷酸的方法和物质 |
US8232049B2 (en) * | 2006-09-29 | 2012-07-31 | Case Western Reserve University | Method for detecting small oligonucleotides |
EP2061901A2 (de) * | 2006-10-31 | 2009-05-27 | Noxxon Pharma AG | Verfahren zum nachweis eines einzel- oder doppelsträngigen nukleinsäuremoleküls |
WO2008154098A2 (en) * | 2007-06-07 | 2008-12-18 | Wisconsin Alumni Research Foundation | Reagents and methods for mirna expression analysis and identification of cancer biomarkers |
WO2009029875A1 (en) * | 2007-08-30 | 2009-03-05 | Bioventures, Inc. | Method for the labeling and detection of small polynucleotides |
US7998677B2 (en) * | 2008-02-26 | 2011-08-16 | Regulus Therapeutics, Inc. | MicroRNA detection |
GB0807321D0 (en) * | 2008-04-22 | 2008-05-28 | Oxford Gene Tech Ip Ltd | Analysis of nucleic acids |
GB2496016B (en) | 2011-09-09 | 2016-03-16 | Univ Leland Stanford Junior | Methods for obtaining a sequence |
WO2013096839A1 (en) | 2011-12-22 | 2013-06-27 | Somagenics, Inc. | Methods of constructing small rna libraries and their use for expression profiling of target rnas |
US9637781B2 (en) * | 2012-02-14 | 2017-05-02 | The Johns Hopkins University | MiRNA analysis methods |
WO2014134320A1 (en) * | 2013-03-01 | 2014-09-04 | Somagenics, Inc. | Methods, compositions and systems for the analysis of nucleic acid molecules |
JP6435334B2 (ja) | 2013-12-11 | 2018-12-05 | アキュラジェン ホールディングス リミテッド | 稀な配列変異体を検出するための組成物および方法 |
US11286519B2 (en) | 2013-12-11 | 2022-03-29 | Accuragen Holdings Limited | Methods and compositions for enrichment of amplification products |
US11859246B2 (en) | 2013-12-11 | 2024-01-02 | Accuragen Holdings Limited | Methods and compositions for enrichment of amplification products |
CN114807323A (zh) | 2015-10-09 | 2022-07-29 | 安可济控股有限公司 | 用于富集扩增产物的方法及组合物 |
US20180363039A1 (en) * | 2015-12-03 | 2018-12-20 | Accuragen Holdings Limited | Methods and compositions for forming ligation products |
WO2017112666A1 (en) | 2015-12-21 | 2017-06-29 | Somagenics, Inc. | Methods of library construction for polynucleotide sequencing |
US11427866B2 (en) | 2016-05-16 | 2022-08-30 | Accuragen Holdings Limited | Method of improved sequencing by strand identification |
WO2018035170A1 (en) | 2016-08-15 | 2018-02-22 | Accuragen Holdings Limited | Compositions and methods for detecting rare sequence variants |
CN107267604B (zh) * | 2017-06-08 | 2021-02-26 | 中山大学 | 一种基于短链核酸探针和双链特异性核酸内切酶的高特异性microRNA荧光检测方法 |
US11203782B2 (en) | 2018-03-29 | 2021-12-21 | Accuragen Holdings Limited | Compositions and methods comprising asymmetric barcoding |
JP2021526798A (ja) | 2018-06-06 | 2021-10-11 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアThe Regents Of The University Of California | 核酸ライブラリを生成する方法ならびにそれを実施するための組成物およびキット |
WO2019241290A1 (en) * | 2018-06-12 | 2019-12-19 | Accuragen Holdings Limited | Methods and compositions for forming ligation products |
CA3199080A1 (en) * | 2020-10-26 | 2022-05-05 | Eclipse Bioinnovations, Inc. | Methods and kits for enriching for polynucleotides |
WO2023023584A2 (en) | 2021-08-19 | 2023-02-23 | Eclipse Bioinnovations, Inc. | Methods for detecting rna binding protein complexes |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5801154A (en) * | 1993-10-18 | 1998-09-01 | Isis Pharmaceuticals, Inc. | Antisense oligonucleotide modulation of multidrug resistance-associated protein |
US6534273B2 (en) * | 1997-05-02 | 2003-03-18 | Gen-Probe Incorporated | Two-step hybridization and capture of a polynucleotide |
US20040175732A1 (en) | 2002-11-15 | 2004-09-09 | Rana Tariq M. | Identification of micrornas and their targets |
US20050009059A1 (en) * | 2003-05-07 | 2005-01-13 | Affymetrix, Inc. | Analysis of methylation status using oligonucleotide arrays |
WO2005003318A2 (en) | 2003-07-02 | 2005-01-13 | Perkinelmer Las, Inc. | Assay and process for labeling and detection of micro rna and small interfering rna sequences |
US8192937B2 (en) * | 2004-04-07 | 2012-06-05 | Exiqon A/S | Methods for quantification of microRNAs and small interfering RNAs |
WO2006033020A2 (en) | 2004-04-26 | 2006-03-30 | Rosetta Genomics Ltd. | Methods and apparatus for the detection and validation of micrornas |
US20060019258A1 (en) * | 2004-07-20 | 2006-01-26 | Illumina, Inc. | Methods and compositions for detection of small interfering RNA and micro-RNA |
US8278035B2 (en) * | 2005-08-19 | 2012-10-02 | Bioventures, Inc. | Method and substances for isolating miRNAs |
CN101528763B (zh) * | 2006-08-30 | 2012-06-27 | 生物风险公司 | 分离和检测小多核苷酸的方法和物质 |
-
2006
- 2006-08-18 US US10/593,383 patent/US8278035B2/en active Active
- 2006-08-18 CA CA2613136A patent/CA2613136C/en not_active Expired - Fee Related
- 2006-08-18 EP EP06789847A patent/EP1922420B1/de not_active Not-in-force
- 2006-08-18 AT AT06789847T patent/ATE455871T1/de not_active IP Right Cessation
- 2006-08-18 WO PCT/US2006/032264 patent/WO2007024653A2/en active Search and Examination
- 2006-08-18 DE DE602006011917T patent/DE602006011917D1/de active Active
-
2008
- 2008-01-06 IL IL188599A patent/IL188599A/en not_active IP Right Cessation
-
2012
- 2012-08-29 US US13/598,003 patent/US8524448B2/en active Active
-
2013
- 2013-07-30 US US13/954,740 patent/US9109224B2/en active Active
-
2015
- 2015-06-19 US US14/744,749 patent/US20150284797A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
US9109224B2 (en) | 2015-08-18 |
CA2613136A1 (en) | 2007-03-01 |
US20080160511A1 (en) | 2008-07-03 |
CA2613136C (en) | 2011-11-08 |
EP1922420A4 (de) | 2008-11-26 |
WO2007024653A2 (en) | 2007-03-01 |
US8524448B2 (en) | 2013-09-03 |
IL188599A0 (en) | 2008-04-13 |
US20130338011A1 (en) | 2013-12-19 |
US20120329054A1 (en) | 2012-12-27 |
US8278035B2 (en) | 2012-10-02 |
WO2007024653A3 (en) | 2008-04-10 |
EP1922420A2 (de) | 2008-05-21 |
IL188599A (en) | 2012-05-31 |
ATE455871T1 (de) | 2010-02-15 |
EP1922420B1 (de) | 2010-01-20 |
US20150284797A1 (en) | 2015-10-08 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |