JP2007504839A - 油性酵母菌における多不飽和脂肪酸の生成 - Google Patents

油性酵母菌における多不飽和脂肪酸の生成 Download PDF

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JP2007504839A
JP2007504839A JP2006532907A JP2006532907A JP2007504839A JP 2007504839 A JP2007504839 A JP 2007504839A JP 2006532907 A JP2006532907 A JP 2006532907A JP 2006532907 A JP2006532907 A JP 2006532907A JP 2007504839 A JP2007504839 A JP 2007504839A
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acid
desaturase
gene encoding
yeast
gene
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JP2007504839A5 (enANDdq=inassignee:"E.I. Du Pont De Nemours And Company" productionANDhl=enANDsa=XANDved=0ahUKEwinwuLZxaTSAhVo6YMKHarwDmIQ6AEIITAB
Inventor
ピカタジオ，ステイーブン・ケイ
ヤダブ，ナレンドラ・エス
ズー，シン・サン
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EIDP Inc
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EI Du Pont de Nemours and Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
JP2006532907A 2003-05-07 2004-05-07 油性酵母菌における多不飽和脂肪酸の生成 Pending JP2007504839A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46867703P 2003-05-07 2003-05-07
PCT/US2004/014541 WO2004101757A2 (en) 2003-05-07 2004-05-07 Production of polyunsaturated fatty acids in oleaginous yeasts

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JP2007504839A true JP2007504839A (ja) 2007-03-08
JP2007504839A5 JP2007504839A5 (enANDdq=inassignee:"E.I. Du Pont De Nemours And Company" productionANDhl=enANDsa=XANDved=0ahUKEwinwuLZxaTSAhVo6YMKHarwDmIQ6AEIITAB) 2007-06-28

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US (5) US7238482B2 (enANDdq=inassignee:"E.I. Du Pont De Nemours And Company" productionANDhl=enANDsa=XANDved=0ahUKEwinwuLZxaTSAhVo6YMKHarwDmIQ6AEIITAB)
EP (4) EP2392664B1 (enANDdq=inassignee:"E.I. Du Pont De Nemours And Company" productionANDhl=enANDsa=XANDved=0ahUKEwinwuLZxaTSAhVo6YMKHarwDmIQ6AEIITAB)
JP (1) JP2007504839A (enANDdq=inassignee:"E.I. Du Pont De Nemours And Company" productionANDhl=enANDsa=XANDved=0ahUKEwinwuLZxaTSAhVo6YMKHarwDmIQ6AEIITAB)
KR (1) KR20060018832A (enANDdq=inassignee:"E.I. Du Pont De Nemours And Company" productionANDhl=enANDsa=XANDved=0ahUKEwinwuLZxaTSAhVo6YMKHarwDmIQ6AEIITAB)
CN (4) CN101824441A (enANDdq=inassignee:"E.I. Du Pont De Nemours And Company" productionANDhl=enANDsa=XANDved=0ahUKEwinwuLZxaTSAhVo6YMKHarwDmIQ6AEIITAB)
BR (1) BRPI0410688A (enANDdq=inassignee:"E.I. Du Pont De Nemours And Company" productionANDhl=enANDsa=XANDved=0ahUKEwinwuLZxaTSAhVo6YMKHarwDmIQ6AEIITAB)
CA (1) CA2526935C (enANDdq=inassignee:"E.I. Du Pont De Nemours And Company" productionANDhl=enANDsa=XANDved=0ahUKEwinwuLZxaTSAhVo6YMKHarwDmIQ6AEIITAB)
DK (3) DK2402448T3 (enANDdq=inassignee:"E.I. Du Pont De Nemours And Company" productionANDhl=enANDsa=XANDved=0ahUKEwinwuLZxaTSAhVo6YMKHarwDmIQ6AEIITAB)
NO (1) NO20055784L (enANDdq=inassignee:"E.I. Du Pont De Nemours And Company" productionANDhl=enANDsa=XANDved=0ahUKEwinwuLZxaTSAhVo6YMKHarwDmIQ6AEIITAB)
WO (1) WO2004101757A2 (enANDdq=inassignee:"E.I. Du Pont De Nemours And Company" productionANDhl=enANDsa=XANDved=0ahUKEwinwuLZxaTSAhVo6YMKHarwDmIQ6AEIITAB)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010158219A (ja) * 2009-01-09 2010-07-22 Univ Of Yamanashi 油脂生産菌の培養方法
JP2012501185A (ja) * 2008-08-29 2012-01-19 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 油性生物中の含油量の変更のためのｓｎｆ１タンパク質キナーゼ活性の操作

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110059496A1 (en) * 2003-06-25 2011-03-10 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
US7459546B2 (en) * 2003-06-25 2008-12-02 E.I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase regulatory sequences for gene expression in oleaginous yeast
US7267976B2 (en) * 2003-07-02 2007-09-11 E.I. Du Pont De Nemours And Company Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts
CA2568689A1 (en) * 2004-06-04 2005-12-15 Fluxome Sciences A/S Metabolically engineered cells for the production of polyunsaturated fatty acids
AU2005267202B2 (en) * 2004-06-25 2011-10-27 Corteva Agriscience Llc Delta-8 desaturase and its use in making polyunsaturated fatty acids
US7465564B2 (en) * 2004-08-10 2008-12-16 E.I. Du Pont De Nemours And Company Production of γ-linolenic acid in oleaginous yeast
US7264949B2 (en) 2004-09-15 2007-09-04 E.I. Du Pont De Nemours And Company Glycerol-3-phosphate o-acyltransferase promoter for gene expression in oleaginous yeast
US7192762B2 (en) 2004-11-04 2007-03-20 E. I. Du Pont De Nemours And Company Mortierella alpina glycerol-3-phosphate o-acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US8685679B2 (en) 2004-11-04 2014-04-01 E I Du Pont De Nemours And Company Acyltransferase regulation to increase the percent of polyunsaturated fatty acids in total lipids and oils of oleaginous organisms
US7273746B2 (en) 2004-11-04 2007-09-25 E.I. Dupont De Nemours And Company Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7588931B2 (en) 2004-11-04 2009-09-15 E. I. Du Pont De Nemours And Company High arachidonic acid producing strains of Yarrowia lipolytica
US20060094102A1 (en) 2004-11-04 2006-05-04 Zhixiong Xue Ammonium transporter promoter for gene expression in oleaginous yeast
US7189559B2 (en) * 2004-11-04 2007-03-13 E. I. Du Pont De Nemours And Company Mortierella alpina lysophosphatidic acid acyltransferase homolog for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
WO2006064317A1 (en) * 2004-12-14 2006-06-22 Avestha Gengraine Technologies Pvt. Ltd. Recombinant production docosahexaenoic acid (dha) in yeast
DE102004062141A1 (de) * 2004-12-23 2006-07-06 Nutrinova Nutrition Specialties & Food Ingredients Gmbh Verfahren zur Herstellung eines Rohöls aus Gemischen von Mikroorganismen und Pflanzen, das so hergestellte Öl sowie die spezifischen Verwendungen des so hergestellten und gegebenenfalls zusätzlich raffinierten Öls
AU2006227165B2 (en) 2005-03-18 2011-11-10 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
US7470532B2 (en) 2005-10-19 2008-12-30 E.I. Du Pont De Nemours And Company Mortierella alpina C16/18 fatty acid elongase
CA2625855C (en) 2005-11-23 2016-04-19 E. I. Du Pont De Nemours And Company Delta-9 elongases and their use in making polyunsaturated fatty acids
US7465793B2 (en) 2006-04-20 2008-12-16 E.I. Du Pont De Nemours And Company Synthetic Δ17 desaturase derived from Phytophthora ramourum and its use in making polyunsaturated fatty acids
US7943823B2 (en) 2006-04-28 2011-05-17 E.I. Du Pont De Nemours And Company Delta-8 desaturase and its use in making polyunsaturated fatty acids
EP2013335A2 (en) 2006-05-01 2009-01-14 E.I. Du Pont De Nemours And Company Fungal delta12 desaturase and delta15 desaturase motifs
US7695950B2 (en) 2006-05-17 2010-04-13 E. I. Du Pont De Nemours And Company Δ5 desaturase and its use in making polyunsaturated fatty acids
US7678560B2 (en) * 2006-05-17 2010-03-16 E.I. Du Pont De Nemours And Company Δ 5 desaturase and its use in making polyunsaturated fatty acids
WO2008042338A2 (en) 2006-09-28 2008-04-10 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
EP2390339B1 (en) 2006-10-23 2013-07-31 E. I. du Pont de Nemours and Company Delta-8 desaturases and their use in making polyunsaturated fatty acids
DK2087105T3 (da) * 2006-10-30 2015-05-26 Du Pont Delta 17-desaturase og anvendelse heraf ved fremstilling af flerumættede fedtsyrer
US7709239B2 (en) * 2006-12-07 2010-05-04 E.I. Du Pont De Nemours And Company Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
US8846374B2 (en) 2006-12-12 2014-09-30 E I Du Pont De Nemours And Company Carotenoid production in a recombinant oleaginous yeast
CA2675207A1 (en) 2007-02-12 2008-07-21 E. I. Du Pont De Nemours And Company Production of arachidonic acid in oilseed plants
ES2559312T3 (es) * 2007-04-03 2016-02-11 E. I. Du Pont De Nemours And Company Multienzimas y su uso en la fabricación de ácidos grasos poliinsaturados
US7790156B2 (en) * 2007-04-10 2010-09-07 E. I. Du Pont De Nemours And Company Δ-8 desaturases and their use in making polyunsaturated fatty acids
US7794701B2 (en) 2007-04-16 2010-09-14 E.I. Du Pont De Nemours And Company Δ-9 elongases and their use in making polyunsaturated fatty acids
US8957280B2 (en) * 2007-05-03 2015-02-17 E. I. Du Pont De Nemours And Company Delta-5 desaturases and their use in making polyunsaturated fatty acids
WO2008151149A2 (en) * 2007-06-01 2008-12-11 Solazyme, Inc. Production of oil in microorganisms
US8673623B2 (en) * 2007-08-31 2014-03-18 Board Of Regents, The University Of Texas System Apparatus for performing magnetic electroporation
CN113846129A (zh) 2007-09-12 2021-12-28 帝斯曼知识产权资产管理有限公司 生物油及其制备与应用
CA2701094A1 (en) 2007-10-03 2009-04-09 E. I. Du Pont De Nemours And Company Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production
US20120149886A1 (en) * 2008-04-10 2012-06-14 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
US8168858B2 (en) 2008-06-20 2012-05-01 E. I. Du Pont De Nemours And Company Delta-9 fatty acid elongase genes and their use in making polyunsaturated fatty acids
JP5762291B2 (ja) 2008-09-19 2015-08-12 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 変異δ５デサチュラーゼと、多価不飽和脂肪酸の製造におけるそれらの使用
EP3260544A1 (en) 2008-11-18 2017-12-27 Commonwealth Scientific and Industrial Research Organisation Enzymes and methods for producing omega-3 fatty acids
US8802924B2 (en) * 2008-12-09 2014-08-12 Ben Gurion University Of The Negev Research And Development Authority Polyunsaturated fatty acid elongase
AU2009335903B2 (en) 2008-12-18 2016-04-14 E. I. Du Pont De Nemours And Company Reducing byproduction of malonates in a fermentation process
FR2940315B1 (fr) * 2008-12-19 2012-12-14 Airbus France Nouveau procede de culture de levures du genre yarrowia
DE102009007402A1 (de) 2009-02-04 2010-08-05 VLB Berlin e. V. Institut für Mikrobiologie Biosynthese und Gewinnung von Substanzen aus Zellen
US8916154B2 (en) * 2009-04-27 2014-12-23 Abbott Laboratories Antibodies against delta-5 desaturase and uses thereof
EP2442666B1 (en) 2009-06-16 2015-04-08 E. I. du Pont de Nemours and Company High eicosapentaenoic acid oils from improved optimized strains of yarrowia lipolytica
BRPI1009649A2 (pt) 2009-06-16 2016-03-15 Du Pont célula hospedeira yarrowia sp, recombinante, método para fabricar um óleo microbiano e célula hospedeira recombinante para produção de um óleo
AU2010260227B2 (en) 2009-06-16 2015-01-29 E. I. Du Pont De Nemours And Company Improvement of long chain omega-3 and omega-6 polyunsaturated fatty acid biosynthesis by expression of acyl-CoA lysophospholipid acyltransferases
US8399226B2 (en) * 2009-06-16 2013-03-19 E I Du Pont De Nemours And Company High eicosapentaenoic acid oils from improved optimized strains of Yarrowia lipolytica
US20110065161A1 (en) * 2009-09-14 2011-03-17 Board Of Regents, The University Of Texas System Bipolar solid state marx generator
CN104982920A (zh) * 2009-11-03 2015-10-21 帝斯曼知识产权资产管理有限公司 包含细胞和具有至少20个碳原子的多不饱和脂肪酸(lc-pufa)的组合物
JP6476436B2 (ja) * 2009-11-03 2019-03-06 ディーエスエム アイピー アセッツ ビー．ブイ．Ｄｓｍ Ｉｐ Ａｓｓｅｔｓ Ｂ．Ｖ． 細胞および少なくとも２０個の炭素原子を有する多価不飽和脂肪酸（ｌｃ−ｐｕｆａ）を含んでなる組成物
US9029108B2 (en) * 2009-11-06 2015-05-12 Diversified Technologies, Inc. Pulsed electric field (PEF) method for continuous enhanced extraction of oil and lipids from small aquatic plants
CA2784711A1 (en) 2009-12-24 2011-06-30 E.I. Du Pont De Nemours And Company Plant membrane bound o-acyl transferase (mboat) family protein sequences and their uses for altering fatty acid compositions
US20110178105A1 (en) 2010-01-15 2011-07-21 E.I. Du Pont De Nemours And Company Clinical benefits of eicosapentaenoic acid in humans
JP2013530679A (ja) * 2010-03-31 2013-08-01 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー トランスジェニック微生物における、目的の非天然生成物の生成を高めるペントースリン酸経路のアップレギュレーション
JP2013525549A (ja) 2010-04-22 2013-06-20 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 微生物バイオマスから多価不飽和脂肪酸含有組成物を得る方法
EP2575486B1 (en) 2010-05-28 2021-09-01 Corbion Biotech, Inc. Food compositions comprising tailored oils
WO2012021686A1 (en) 2010-08-11 2012-02-16 E. I. Du Pont De Nemours And Company Improved aquaculture meat products
AU2011289384A1 (en) 2010-08-11 2013-01-24 E. I. Du Pont De Nemours And Company A sustainable aquaculture feeding strategy
US20120040076A1 (en) 2010-08-11 2012-02-16 E. I. Du Pont De Nemours And Company Aquaculture feed compositions
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EP2609201B1 (en) 2010-08-26 2016-03-02 E. I. du Pont de Nemours and Company Mutant delta-9 elongases and their use in making polyunsaturated fatty acids
CA3024641A1 (en) 2010-11-03 2012-05-10 Corbion Biotech, Inc. Microbial oils with lowered pour points, dielectric fluids produced therefrom, and related methods
JP2014505472A (ja) 2010-12-30 2014-03-06 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 油性酵母中のｙａｐ１転写因子活性を増大させることにより増加した含油率
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SG192594A1 (en) 2011-02-02 2013-09-30 Solazyme Inc Tailored oils produced from recombinant oleaginous microorganisms
US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
US8999663B2 (en) 2011-02-11 2015-04-07 E L Du Pont De Nemours And Company Method for obtaining a lipid-containing composition from microbial biomass
WO2012135773A1 (en) 2011-03-31 2012-10-04 E. I. Du Pont De Nemours And Company Yarrowia diacylglycerol acyltransferase promoter regions for gene expression in yeast
US20120247066A1 (en) 2011-04-01 2012-10-04 Ice House America, Llc Ice bagging apparatus and methods
US8906650B2 (en) 2011-04-01 2014-12-09 E I Du Pont De Nemours And Company Yarrowia esterase/lipase promoter regions for gene expression in yeast
EP2694657A1 (en) 2011-04-05 2014-02-12 E. I. Du Pont de Nemours and Company Yarrowia n-alkane-hydroxylating cytochrome p450 promoter regions for gene expression in yeast
WO2012138612A1 (en) 2011-04-07 2012-10-11 E. I. Du Pont De Nemours And Company Yarrowia peroxisomal 2,4-dienoyl-coa reductase promoter regions for gene expression in yeast
CN103562217A (zh) 2011-05-26 2014-02-05 纳幕尔杜邦公司 在重组含油微生物中表达油体钙蛋白以提高其中的油含量
WO2013024527A1 (ja) * 2011-08-15 2013-02-21 トヨタ自動車株式会社 アルカンの製造方法及びアルカン合成能を有する組換え微生物
WO2013071226A1 (en) * 2011-11-11 2013-05-16 Genomatica, Inc. Eukaryotic organisms and methods for increasing the availability of cytosolic acetyl-coa, and for producing 1,3-butanediol
AU2012327162A1 (en) 2011-12-27 2013-07-11 Commonwealth Scientific And Industrial Research Organisation Production of dihydrosterculic acid and derivatives thereof
KR20150001830A (ko) 2012-04-18 2015-01-06 솔라짐, 인코포레이티드 맞춤 오일
CA2876519C (en) 2012-06-15 2020-09-15 Commonwealth Scientific And Industrial Research Organisation Production of long chain polyunsaturated fatty acids in plant cells
DK2861727T3 (en) 2012-06-19 2018-06-14 Du Pont MUTERED ACYL-COA: LYSOPHOSPHATIDYLCHOLINE ACYL TRANSFERASES
CN102787128A (zh) * 2012-08-28 2012-11-21 江南大学 一种用于多不饱和脂肪酸生物合成的脱饱和酶Δ9 Des
WO2014100062A1 (en) 2012-12-21 2014-06-26 E. I. Du Pont De Nemours And Company Recombinant microbial cells that produce at least 28% eicosapentaenoic acid as dry cell weight
BR112015014844A2 (pt) 2012-12-21 2019-01-15 Du Pont célula microbiana recombinante e método de produção de um óleo microbiano
JP6538564B2 (ja) 2012-12-24 2019-07-03 クオリタス ヘルス リミテッド エイコサペンタエン酸（ｅｐａ）製剤
US10731169B2 (en) 2013-07-05 2020-08-04 Basf Plant Science Company Gmbh Gene expression or activity enhancing elements
BR112016006839A8 (pt) 2013-10-04 2017-10-03 Solazyme Inc Óleos customizados
DE102014220186B4 (de) 2013-12-12 2017-06-22 Technische Universität Dresden Hefestämme und Verfahren zur Produktion von Omega-Hydroxyfettsäuren und Dicarbonsäuren
SG11201604871VA (en) 2013-12-18 2016-07-28 Commw Scient Ind Res Org Lipid comprising long chain polyunsaturated fatty acids
CA2953008C (en) 2014-06-27 2024-03-19 Nuseed Pty Ltd Lipid comprising docosapentaenoic acid
WO2016007862A2 (en) 2014-07-10 2016-01-14 Solazyme, Inc. Novel ketoacyl acp synthase genes and uses thereof
JP6898915B2 (ja) 2015-07-22 2021-07-07 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 生物を用いる長鎖ジカルボン酸の高レベルの生産
CN105181832A (zh) * 2015-08-31 2015-12-23 广州金域医学检验中心有限公司 一种快速检测全血红细胞二十烷二烯酸的方法
JP6897917B2 (ja) 2015-10-01 2021-07-07 ディーエスエム アイピー アセッツ ビー．ブイ．Ｄｓｍ Ｉｐ Ａｓｓｅｔｓ Ｂ．Ｖ． ペットフードに用いるサプリメント素材
WO2019140052A1 (en) 2018-01-10 2019-07-18 Brightseed, Inc. Method for modulating metabolism
US11946093B2 (en) * 2018-10-17 2024-04-02 Archer-Daniels-Midland Company Enzyme addition to omega 3 fermentation broth for the reduction of oligosaccharides through sterilized dextrose solution
CN110373436B (zh) * 2018-12-11 2022-09-23 山东理工大学 一种产双高-γ-亚麻酸卷枝毛霉细胞工厂的构建方法及发酵技术
CN110229832A (zh) * 2019-06-16 2019-09-13 山东理工大学 一种提高耶式解脂酵母生物量和细胞壁产量的菌株和方法
AU2020323503B2 (en) 2019-07-29 2024-02-08 Brightseed, Inc. Method for improving digestive health
US11572546B2 (en) 2019-07-29 2023-02-07 Ginkgo Bioworks, Inc. Methods and compositions involving promoters derived from Yarrowia lipolytica
AU2020383340A1 (en) 2019-11-11 2022-06-09 Brightseed, Inc Extract, consumable product and method for enriching bioactive metabolite in an extract
WO2021130078A1 (en) 2019-12-23 2021-07-01 Dsm Ip Assets B.V. Aquaculture feed
WO2022115503A1 (en) 2020-11-25 2022-06-02 Dupont Nutrition Biosciences Aps Treatment and prevention of coronavirus infection
WO2022129592A1 (en) 2020-12-17 2022-06-23 Dsm Ip Assets B.V. Aquaculture feed
WO2023148154A1 (en) 2022-02-01 2023-08-10 Dsm Ip Assets B.V. Aquaculture feed

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001523092A (ja) * 1997-04-11 2001-11-20 カルジーン エル エル シー 長鎖多不飽和脂肪酸の合成のための方法および組成物
JP2001523091A (ja) * 1997-04-11 2001-11-20 カルジーン エル エル シー 長鎖多不飽和脂肪酸の合成のための方法および組成物
JP2002523098A (ja) * 1998-09-02 2002-07-30 アボット・ラボラトリーズ エロンガーゼ遺伝子及びそれらの使用

Family Cites Families (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ190194A (en) 1978-05-08 1982-02-23 Cpc International Inc Production of oil by fermenting yeast in fatty acids
US5071764A (en) * 1983-10-06 1991-12-10 Pfizer Inc. Process for integrative transformation of yarrowia lipolytica
US4880741A (en) * 1983-10-06 1989-11-14 Pfizer Inc. Process for transformation of Yarrowia lipolytica
GB8407195D0 (en) 1984-03-20 1984-04-26 Cadbury Schweppes Plc Microbial desaturase enzyme inhibitors
US4792523A (en) 1984-08-31 1988-12-20 Cetus Corporation 3'Expression enhancing fragments and method
DD227448A1 (de) 1984-10-18 1985-09-18 Akad Wissenschaften Ddr Verfahren zur herstellung von zitronensaeure auf mikrobiellem wege
US4677072A (en) * 1985-02-28 1987-06-30 Westvaco Corporation Rhodotorula having desaturase enzymes
GB8507058D0 (en) 1985-03-19 1985-04-24 Efamol Ltd Pharmaceutical & dietary compositions
GB8524276D0 (en) * 1985-10-02 1985-11-06 Efamol Ltd Pharmaceutical & dietary compositions
GB8524275D0 (en) * 1985-10-02 1985-11-06 Efamol Ltd Pharmaceutical & dietary compositions
US5107065A (en) 1986-03-28 1992-04-21 Calgene, Inc. Anti-sense regulation of gene expression in plant cells
JP2527340B2 (ja) 1986-12-15 1996-08-21 アプライド バイオシステムズ インコーポレイテッド ロ―ダミン染料の５−及び６−スクシニミジルカルボキシレ―ト異性体
DD259637A1 (de) 1987-04-09 1988-08-31 Adw Ddr Verfahren zur herstellung von isozitratlyase in rekombinanten mikroorganismen
SU1454852A1 (ru) 1987-05-19 1989-01-30 Институт Микробиологии Им.Августа Кирхенштейна Штамм дрожжей JaRRoWIa LIроLYтIса-продуцент липазы и питательна среда дл его культивировани
DD267999B5 (de) 1988-01-06 2000-08-10 Akad Wissenschaften Ddr Verfahren zur herstellung von 2-oxoglutarsaeure durch hefen
US5116871A (en) * 1988-09-13 1992-05-26 Efamol Holdings Plc Fatty acid therapy and compositions for the treatment of myalgic encephalomyelitis
DD275480A1 (de) 1988-09-15 1990-01-24 Ve Forschungszentrum Biotechno Verfahren zur herstellung von zitronensaeure durch aerobe submerse kultivierung von hefen
US5057419A (en) * 1988-09-22 1991-10-15 Rutgers University Genetically engineered plasmid and organisms for the production of specialized oils
DD279267A1 (de) 1989-01-05 1990-05-30 Akad Wissenschaften Ddr Verfahren zur herstellung mikrobieller lipase
PL160027B1 (pl) 1989-03-23 1993-01-29 Akad Rolnicza Sposób jednoczesnego otrzymywania kwasu cytrynowego i kwasu izocytrynowego PL
DD285370A5 (de) 1989-06-29 1990-12-12 Ingenieurtechnik Im Stammbetrieb Veb Kombinat Suesswaren,Dd Verfahren zur immobilisierung von mikroorganismen bzw. coimmobilisierung von mikroorganismen und enzymen
DD285372A5 (de) 1989-06-29 1990-12-12 Ingenieurtechnik,Dd Verfahren zur immobilisierung von mikroorganismen bzw. coimmobilisierung von mikroorganismen und enzymen
US5366860A (en) 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
US5407957A (en) * 1990-02-13 1995-04-18 Martek Corporation Production of docosahexaenoic acid by dinoflagellates
ATE241007T1 (de) 1990-03-16 2003-06-15 Calgene Llc Dnas, die für pflanzliche desaturasen kodieren und deren anwendungen
US5244921A (en) * 1990-03-21 1993-09-14 Martek Corporation Eicosapentaenoic acids and methods for their production
EP0537178B2 (en) * 1990-05-25 2007-06-13 E.I. Du Pont De Nemours And Company Nucleotide sequence of soybean stearoyl-acp desaturase gene
US5658767A (en) * 1991-01-24 1997-08-19 Martek Corporation Arachidonic acid and methods for the production and use thereof
CA2124673C (en) 1991-12-04 2008-08-05 John Browse Fatty acid desaturase genes from plants
EP0578388B1 (en) 1992-06-25 1998-11-04 INTERNATIONAL FLAVORS & FRAGRANCES INC. Fermentation process for preparing 10-hydroxy-C18-carboxylic acid and gamma-dodecalactone derivatives
IT1256043B (it) 1992-08-12 1995-11-21 Ceppo mutante di yarrowia lipolytica e procedimento per la produzione di acido citrico
ES2198408T3 (es) 1992-11-17 2004-02-01 E.I. Du Pont De Nemours And Company Genes para delta 12 desaturasas de acidos grasos microsomales y enzimas afines de plantas.
FR2734843A1 (fr) 1995-06-02 1996-12-06 Centre Nat Rech Scient Nouveau procede de bioconversion microbienne.
EP0770683A1 (en) 1995-10-04 1997-05-02 Mitsubishi Chemical Corporation Method for producing erythritol
JPH09252790A (ja) 1996-03-21 1997-09-30 Japan Energy Corp 発酵法によるピルビン酸の製造方法
WO1997043362A1 (en) * 1996-05-15 1997-11-20 Gist-Brocades B.V. Sterol extraction with polar solvent to give low sterol, high triglyceride, microbial oil
WO1997044470A1 (en) 1996-05-21 1997-11-27 Novo Nordisk A/S Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast
RU2090611C1 (ru) 1996-09-27 1997-09-20 Финогенова Татьяна Васильевна Штамм дрожжей yarrowia lipolytica - продуцент лимонной кислоты, способ получения лимонной кислоты и способ выделения цитрата натрия
US6075183A (en) * 1997-04-11 2000-06-13 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids in plants
JP2002503447A (ja) 1997-12-04 2002-02-05 スミスクライン・ビーチャム・コーポレイション 発現可能なアンチセンス配列を用いる条件発現変異細胞の取得方法
US6677145B2 (en) * 1998-09-02 2004-01-13 Abbott Laboratories Elongase genes and uses thereof
EP0997533A1 (de) 1998-10-24 2000-05-03 Haarmann & Reimer Gmbh Verfahren zur Gewinnung von Gamma-Decalacton
DE00925351T1 (de) 2000-04-28 2004-04-15 Laboratoires Mayoly Spindler Klonierung und expression einer extrazellulären säurebeständigen lipase aus yarrowia lipolytica
US7083972B2 (en) 2000-05-19 2006-08-01 Kaneka Corporation Transformant and process for producing polyester by using the same
JP4347572B2 (ja) 2001-01-25 2009-10-21 アボット・ラボラトリーズ デサチュラーゼ遺伝子とその使用
JP2002296554A (ja) 2001-03-30 2002-10-09 Minebea Co Ltd ファラデー回転子
US7045683B2 (en) 2001-05-04 2006-05-16 Abbott Laboratories Δ4-desaturase genes and uses thereof
US7211656B2 (en) * 2002-01-30 2007-05-01 Abbott Laboratories Desaturase genes, enzymes encoded thereby, and uses thereof
BRPI0311324B1 (pt) 2002-05-22 2020-01-28 Monsanto Technology Llc polinucleotídeo, vetor, polipeptídeo com atividade dessasturase, métodos para produção de planta e óleo de milho contendo ácidos graxos de ômega-3, para aumentar o valor nutricional de um produto comestível, de fabricação de alimento ou alimentação, bem como composição comestível
US7214491B2 (en) * 2003-05-07 2007-05-08 E. I. Du Pont De Nemours And Company Δ-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts
US7364883B2 (en) * 2004-05-07 2008-04-29 Yeastern Biotech Co., Ltd. Process for producing poly-unsaturated fatty acids by oleaginous yeasts

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001523092A (ja) * 1997-04-11 2001-11-20 カルジーン エル エル シー 長鎖多不飽和脂肪酸の合成のための方法および組成物
JP2001523091A (ja) * 1997-04-11 2001-11-20 カルジーン エル エル シー 長鎖多不飽和脂肪酸の合成のための方法および組成物
JP2002523098A (ja) * 1998-09-02 2002-07-30 アボット・ラボラトリーズ エロンガーゼ遺伝子及びそれらの使用

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
JPN6010011404; Ｂｉｏｃｈｅｍｉｃａｌ　ａｎｄ　Ｂｉｏｐｈｙｓｉｃａｌ　Ｒｅｓｅａｒｃｈ　Ｃｏｍｍｕｎｉｃａｔｉｏｎｓ ｖｏｌ．２６３, 1999, ｐｐ．４７-５１ *
JPN6010011406; Ｅｕｒ．　Ｊ．　Ｂｉｏｃｈｅｍ ｖｏｌ．２６１, 1999, ｐｐ．８１２-８２０ *
JPN6010011409; Ｂｉｏｃｈｅｍｉｃａｌ　Ｓｏｃｉｅｔｙ　Ｔｒａｎｓａｃｔｉｏｎｓ ｖｏｌ．２８　ｐａｒｔ６, 2000, ｐｐ．６３２-６３５ *
JPN6011034965; Ｂｉｏｒｅｓｏｕｒｃｅ　Ｔｅｃｈｎｏｌｏｇｙ ｖｏｌ．８２, 2002, ｐｐ．４３-４９ *
JPN6011034967; Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｃｅｌｌ　Ｂｉｏｌｏｇｙ ｖｏｌ．１３７　ｎｏ．６, 1997, ｐｐ．１２６５-１２７８ *
JPN6011034970; ＹＥＡＳＴ ｖｏｌ．１４, 1998, ｐｐ．１２６７-１２８３ *
JPN6011034974; ＦＥＭＳ　Ｍｉｃｒｏｂｉｏｌｏｇｙ　Ｒｅｖｉｅｗｓ ｖｏｌ．１９, 1997, ｐｐ．２１９-２３７ *
JPN6014019854; Ｎｕｃｌｅｉｃ　Ａｃｉｄｓ　Ｒｅｓｅａｒｃｈ，１９８７年，ｖｏｌ．１５，Ｎｏ．８，ｐｐ．３５８１-３５９３ , 1987

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012501185A (ja) * 2008-08-29 2012-01-19 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 油性生物中の含油量の変更のためのｓｎｆ１タンパク質キナーゼ活性の操作
JP2010158219A (ja) * 2009-01-09 2010-07-22 Univ Of Yamanashi 油脂生産菌の培養方法

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