DK2402448T3 - Preparation of polyunsaturated fatty acids in OIL YEAST - Google Patents

Preparation of polyunsaturated fatty acids in OIL YEAST Download PDF

Info

Publication number
DK2402448T3
DK2402448T3 DK11155007.5T DK11155007T DK2402448T3 DK 2402448 T3 DK2402448 T3 DK 2402448T3 DK 11155007 T DK11155007 T DK 11155007T DK 2402448 T3 DK2402448 T3 DK 2402448T3
Authority
DK
Denmark
Prior art keywords
desaturase
ala
leu
val
gly
Prior art date
Application number
DK11155007.5T
Other languages
Danish (da)
English (en)
Inventor
Stephen K Picataggio
Quinn Qun Zhu
Narendra S Yadav
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Application granted granted Critical
Publication of DK2402448T3 publication Critical patent/DK2402448T3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
DK11155007.5T 2003-05-07 2004-05-07 Preparation of polyunsaturated fatty acids in OIL YEAST DK2402448T3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46867703P 2003-05-07 2003-05-07
EP04751769.3A EP1620543B1 (en) 2003-05-07 2004-05-07 Production of polyunsaturated fatty acids in oleaginous yeasts

Publications (1)

Publication Number Publication Date
DK2402448T3 true DK2402448T3 (en) 2016-05-17

Family

ID=33452224

Family Applications (3)

Application Number Title Priority Date Filing Date
DK11154995.2T DK2392664T3 (en) 2003-05-07 2004-05-07 Preparation of polyunsaturated fatty acids in OIL YEAST
DK11155007.5T DK2402448T3 (en) 2003-05-07 2004-05-07 Preparation of polyunsaturated fatty acids in OIL YEAST
DK04751769.3T DK1620543T3 (en) 2003-05-07 2004-05-07 PREPARATION OF POLYUM Saturated FAT ACIDS IN OIL Yeast

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK11154995.2T DK2392664T3 (en) 2003-05-07 2004-05-07 Preparation of polyunsaturated fatty acids in OIL YEAST

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK04751769.3T DK1620543T3 (en) 2003-05-07 2004-05-07 PREPARATION OF POLYUM Saturated FAT ACIDS IN OIL Yeast

Country Status (10)

Country Link
US (5) US7238482B2 (enANDdq=inassignee:"E.I. Du Pont De Nemours And Company" productionANDhl=enANDsa=XANDved=0ahUKEwinwuLZxaTSAhVo6YMKHarwDmIQ6AEIITAB)
EP (4) EP1620543B1 (enANDdq=inassignee:"E.I. Du Pont De Nemours And Company" productionANDhl=enANDsa=XANDved=0ahUKEwinwuLZxaTSAhVo6YMKHarwDmIQ6AEIITAB)
JP (1) JP2007504839A (enANDdq=inassignee:"E.I. Du Pont De Nemours And Company" productionANDhl=enANDsa=XANDved=0ahUKEwinwuLZxaTSAhVo6YMKHarwDmIQ6AEIITAB)
KR (1) KR20060018832A (enANDdq=inassignee:"E.I. Du Pont De Nemours And Company" productionANDhl=enANDsa=XANDved=0ahUKEwinwuLZxaTSAhVo6YMKHarwDmIQ6AEIITAB)
CN (4) CN101824441A (enANDdq=inassignee:"E.I. Du Pont De Nemours And Company" productionANDhl=enANDsa=XANDved=0ahUKEwinwuLZxaTSAhVo6YMKHarwDmIQ6AEIITAB)
BR (1) BRPI0410688A (enANDdq=inassignee:"E.I. Du Pont De Nemours And Company" productionANDhl=enANDsa=XANDved=0ahUKEwinwuLZxaTSAhVo6YMKHarwDmIQ6AEIITAB)
CA (1) CA2526935C (enANDdq=inassignee:"E.I. Du Pont De Nemours And Company" productionANDhl=enANDsa=XANDved=0ahUKEwinwuLZxaTSAhVo6YMKHarwDmIQ6AEIITAB)
DK (3) DK2392664T3 (enANDdq=inassignee:"E.I. Du Pont De Nemours And Company" productionANDhl=enANDsa=XANDved=0ahUKEwinwuLZxaTSAhVo6YMKHarwDmIQ6AEIITAB)
NO (1) NO20055784L (enANDdq=inassignee:"E.I. Du Pont De Nemours And Company" productionANDhl=enANDsa=XANDved=0ahUKEwinwuLZxaTSAhVo6YMKHarwDmIQ6AEIITAB)
WO (1) WO2004101757A2 (enANDdq=inassignee:"E.I. Du Pont De Nemours And Company" productionANDhl=enANDsa=XANDved=0ahUKEwinwuLZxaTSAhVo6YMKHarwDmIQ6AEIITAB)

Families Citing this family (109)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110059496A1 (en) * 2003-06-25 2011-03-10 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
US7459546B2 (en) * 2003-06-25 2008-12-02 E.I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase regulatory sequences for gene expression in oleaginous yeast
US7267976B2 (en) * 2003-07-02 2007-09-11 E.I. Du Pont De Nemours And Company Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts
PT1766023E (pt) * 2004-06-04 2010-12-09 Fluxome Sciences As Células metabolicamente manipuladas para a produção de ácidos gordos poliinsaturados
US7256033B2 (en) * 2004-06-25 2007-08-14 E. I. Du Pont De Nemours And Company Delta-8 desaturase and its use in making polyunsaturated fatty acids
US7465564B2 (en) * 2004-08-10 2008-12-16 E.I. Du Pont De Nemours And Company Production of γ-linolenic acid in oleaginous yeast
US7264949B2 (en) 2004-09-15 2007-09-04 E.I. Du Pont De Nemours And Company Glycerol-3-phosphate o-acyltransferase promoter for gene expression in oleaginous yeast
US7273746B2 (en) 2004-11-04 2007-09-25 E.I. Dupont De Nemours And Company Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7192762B2 (en) 2004-11-04 2007-03-20 E. I. Du Pont De Nemours And Company Mortierella alpina glycerol-3-phosphate o-acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US20060094102A1 (en) 2004-11-04 2006-05-04 Zhixiong Xue Ammonium transporter promoter for gene expression in oleaginous yeast
US7189559B2 (en) * 2004-11-04 2007-03-13 E. I. Du Pont De Nemours And Company Mortierella alpina lysophosphatidic acid acyltransferase homolog for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7588931B2 (en) * 2004-11-04 2009-09-15 E. I. Du Pont De Nemours And Company High arachidonic acid producing strains of Yarrowia lipolytica
US8685679B2 (en) 2004-11-04 2014-04-01 E I Du Pont De Nemours And Company Acyltransferase regulation to increase the percent of polyunsaturated fatty acids in total lipids and oils of oleaginous organisms
CN101124330A (zh) * 2004-12-14 2008-02-13 阿维斯塔金格兰技术有限公司 在酵母中重组生产二十二碳六烯酸（dha）
DE102004062141A1 (de) * 2004-12-23 2006-07-06 Nutrinova Nutrition Specialties & Food Ingredients Gmbh Verfahren zur Herstellung eines Rohöls aus Gemischen von Mikroorganismen und Pflanzen, das so hergestellte Öl sowie die spezifischen Verwendungen des so hergestellten und gegebenenfalls zusätzlich raffinierten Öls
AU2006227165B2 (en) 2005-03-18 2011-11-10 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
US7470532B2 (en) * 2005-10-19 2008-12-30 E.I. Du Pont De Nemours And Company Mortierella alpina C16/18 fatty acid elongase
EP1957641A2 (en) 2005-11-23 2008-08-20 E.I. Du Pont De Nemours And Company Delta-9 elongases and their use in making polyunsaturated fatty acids
US7465793B2 (en) 2006-04-20 2008-12-16 E.I. Du Pont De Nemours And Company Synthetic Δ17 desaturase derived from Phytophthora ramourum and its use in making polyunsaturated fatty acids
US7943823B2 (en) 2006-04-28 2011-05-17 E.I. Du Pont De Nemours And Company Delta-8 desaturase and its use in making polyunsaturated fatty acids
US20070254299A1 (en) 2006-05-01 2007-11-01 Yadav Narendra S Fungal delta 12 desaturase and delta 15 desaturase motifs
US7678560B2 (en) * 2006-05-17 2010-03-16 E.I. Du Pont De Nemours And Company Δ 5 desaturase and its use in making polyunsaturated fatty acids
US7695950B2 (en) * 2006-05-17 2010-04-13 E. I. Du Pont De Nemours And Company Δ5 desaturase and its use in making polyunsaturated fatty acids
EP2078092A2 (en) 2006-09-28 2009-07-15 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
PL2390339T3 (pl) 2006-10-23 2013-12-31 Du Pont Delta-8 desaturazy i ich zastosowanie w wytwarzaniu polinienasyconych kwasów tłuszczowych
DK2087105T3 (da) * 2006-10-30 2015-05-26 Du Pont Delta 17-desaturase og anvendelse heraf ved fremstilling af flerumættede fedtsyrer
US7709239B2 (en) * 2006-12-07 2010-05-04 E.I. Du Pont De Nemours And Company Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
US8846374B2 (en) 2006-12-12 2014-09-30 E I Du Pont De Nemours And Company Carotenoid production in a recombinant oleaginous yeast
EP2121934A2 (en) 2007-02-12 2009-11-25 E. I. Du Pont de Nemours and Company Production of arachidonic acid in oilseed plants
HUE028692T2 (en) * 2007-04-03 2016-12-28 Du Pont Multi-uses and their use in the production of polyunsaturated fatty acids
US8188338B2 (en) 2007-04-10 2012-05-29 E. I. Du Pont De Nemours And Company Delta-8 desaturases and their use in making polyunsaturated fatty acids
US7794701B2 (en) 2007-04-16 2010-09-14 E.I. Du Pont De Nemours And Company Δ-9 elongases and their use in making polyunsaturated fatty acids
US7943365B2 (en) 2007-05-03 2011-05-17 E.I. Du Pont De Nemours And Company Δ-5 desaturases and their use in making polyunsaturated fatty acids
BRPI0811967B1 (pt) * 2007-06-01 2023-01-17 Terravia Holdings, Inc Microalga chlorella ou prototheca e método para produção de lipídios microbianos
WO2009029613A1 (en) * 2007-08-31 2009-03-05 The Board Of Regents, The University Of Texas System Apparatus for performing magnetic electroporation
JP2010538642A (ja) 2007-09-12 2010-12-16 マーテック バイオサイエンシーズ コーポレーション 生物油ならびにその生産および使用
WO2009046231A1 (en) 2007-10-03 2009-04-09 E. I. Du Pont De Nemours And Company Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production
WO2009126890A2 (en) * 2008-04-10 2009-10-15 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
US8168858B2 (en) 2008-06-20 2012-05-01 E. I. Du Pont De Nemours And Company Delta-9 fatty acid elongase genes and their use in making polyunsaturated fatty acids
WO2010025374A2 (en) 2008-08-29 2010-03-04 E. I. Du Pont De Nemours And Company Manipulation of snf1 protein kinase activity for altered oil content in oleaginous organisms
DK2324119T3 (en) 2008-09-19 2016-07-18 Du Pont Mutant DELTA5 Desaturases AND USE THEREOF FOR THE PRODUCTION OF polyunsaturated fatty acids
AR074364A1 (es) 2008-11-18 2011-01-12 Commw Scient Ind Res Org Enzimas y metodo para producir acidos grasos omega -3
WO2010067352A1 (en) * 2008-12-09 2010-06-17 Ben Gurion University Of The Negev Research And Development Authority Polyunsaturated fatty acid elongase
EP2358862B1 (en) 2008-12-18 2017-08-02 E. I. du Pont de Nemours and Company Reducing byproduction of malonates in a fermentation process
FR2940315B1 (fr) * 2008-12-19 2012-12-14 Airbus France Nouveau procede de culture de levures du genre yarrowia
JP5709196B2 (ja) * 2009-01-09 2015-04-30 国立大学法人山梨大学 油脂生産菌の培養方法
DE102009007402A1 (de) 2009-02-04 2010-08-05 VLB Berlin e. V. Institut für Mikrobiologie Biosynthese und Gewinnung von Substanzen aus Zellen
US8916154B2 (en) * 2009-04-27 2014-12-23 Abbott Laboratories Antibodies against delta-5 desaturase and uses thereof
WO2010147904A1 (en) 2009-06-16 2010-12-23 E. I. Du Pont De Nemours And Company Improved optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production
US8399226B2 (en) * 2009-06-16 2013-03-19 E I Du Pont De Nemours And Company High eicosapentaenoic acid oils from improved optimized strains of Yarrowia lipolytica
WO2010147907A1 (en) 2009-06-16 2010-12-23 E. I. Du Pont De Nemours And Company High eicosapentaenoic acid oils from improved optimized strains of yarrowia lipolytica
EP2443248B1 (en) 2009-06-16 2017-12-27 E. I. du Pont de Nemours and Company IMPROVEMENT OF LONG CHAIN OMEGA-3 AND OMEGA-6 POLYUNSATURATED FATTY ACID BIOSYNTHESIS BY EXPRESSION OF ACYL-CoA LYSOPHOSPHOLIPID ACYLTRANSFERASES
WO2011032149A2 (en) * 2009-09-14 2011-03-17 Board Of Regents, The University Of Texas System Bipolar solid state marx generator
CN104982920A (zh) * 2009-11-03 2015-10-21 帝斯曼知识产权资产管理有限公司 包含细胞和具有至少20个碳原子的多不饱和脂肪酸(lc-pufa)的组合物
WO2011054800A1 (en) * 2009-11-03 2011-05-12 Dsm Ip Assets B.V. Composition comprising cells and a polyunsaturated fatty acid having at least 20 carbon atoms (lc-pufa)
US9029108B2 (en) 2009-11-06 2015-05-12 Diversified Technologies, Inc. Pulsed electric field (PEF) method for continuous enhanced extraction of oil and lipids from small aquatic plants
EP2516638A1 (en) 2009-12-24 2012-10-31 E.I. Du Pont De Nemours And Company Plant membrane bound o-acyl transferase (mboat) family protein sequences and their uses for altering fatty acid compositions
US20110178105A1 (en) 2010-01-15 2011-07-21 E.I. Du Pont De Nemours And Company Clinical benefits of eicosapentaenoic acid in humans
CA2790053A1 (en) * 2010-03-31 2011-10-06 E.I. Du Pont De Nemours And Company Pentose phosphate pathway upregulation to increase production of non-native products of interest in transgenic microorganisms
CN103282474A (zh) 2010-04-22 2013-09-04 纳幕尔杜邦公司 从微生物生物质中获得包含多不饱和脂肪酸的组合物的方法
SG10201504197SA (en) 2010-05-28 2015-06-29 Solazyme Inc Food Compositions Comprising Tailored Oils
US20120040076A1 (en) 2010-08-11 2012-02-16 E. I. Du Pont De Nemours And Company Aquaculture feed compositions
CA2805882A1 (en) 2010-08-11 2012-02-16 E. I. Dupont De Nemours And Company Improved aquaculture meat products
CA2805891A1 (en) 2010-08-11 2012-02-16 Scott E. Nichols A sustainable aquaculture feeding strategy
BR112013004356A2 (pt) 2010-08-26 2017-06-27 Du Pont polinucleotídeo isolado, polipeptídeo mutante, constructo recombinante, célula transformada, método para produção de um ácido graxo poli-insaturado, óleo microbiano e célula hospedeira microbiana recombinante.
KR20130138760A (ko) 2010-08-26 2013-12-19 이 아이 듀폰 디 네모아 앤드 캄파니 고농도의 에이코사펜타엔산 생성을 위한 재조합 미생물 숙주 세포
WO2012027698A1 (en) 2010-08-26 2012-03-01 E.I. Du Pont De Nemours And Company Mutant hpgg motif and hdash motif delta-5 desaturases and their use in making polyunsaturated fatty acids
EP2635663B1 (en) 2010-11-03 2019-05-08 Corbion Biotech, Inc. Microbial oils with lowered pour points, dielectric fluids produced therefrom, and related methods
JP2014503216A (ja) 2010-12-30 2014-02-13 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー スクロース利用のための、ヤロウィア・リポリティカ（Ｙａｒｒｏｗｉａｌｉｐｏｌｙｔｉｃａ）におけるサッカロミセス・セレビシア（Ｓａｃｃｈａｒｏｍｙｃｅｓｃｅｒｅｖｉｓｉａｅ）ＳＵＣ２遺伝子の使用
JP2014505472A (ja) 2010-12-30 2014-03-06 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 油性酵母中のｙａｐ１転写因子活性を増大させることにより増加した含油率
CN103827307A (zh) 2011-02-02 2014-05-28 索拉兹米公司 产自重组产油微生物的定制油
US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
US8999663B2 (en) 2011-02-11 2015-04-07 E L Du Pont De Nemours And Company Method for obtaining a lipid-containing composition from microbial biomass
EP2691518A1 (en) 2011-03-31 2014-02-05 E.I. Du Pont De Nemours And Company Yarrowia diacylglycerol acyltransferase promoter regions for gene expression in yeast
US20120247066A1 (en) 2011-04-01 2012-10-04 Ice House America, Llc Ice bagging apparatus and methods
WO2012135777A1 (en) 2011-04-01 2012-10-04 E. I. Du Pont De Nemours And Company Yarrowia esterase/lipase promoter regions for gene expression in yeast
EP2694657A1 (en) 2011-04-05 2014-02-12 E. I. Du Pont de Nemours and Company Yarrowia n-alkane-hydroxylating cytochrome p450 promoter regions for gene expression in yeast
US8609369B2 (en) 2011-04-07 2013-12-17 E I Du Pont De Nemours And Company Yarrowia peroxisomal 2,4-dienoyl-CoA reductase promoter regions for gene expression in yeast
US8785164B2 (en) 2011-05-26 2014-07-22 E I Du Pont De Nemours And Company Expression of caleosin in recombinant oleaginous microorganisms to increase oil content therein
BR112014003546A2 (pt) * 2011-08-15 2017-03-14 Toyota Motor Co Ltd método para a produção de alcano e micro-organismo recombinante capazes de sintetizar alcano
WO2013071226A1 (en) * 2011-11-11 2013-05-16 Genomatica, Inc. Eukaryotic organisms and methods for increasing the availability of cytosolic acetyl-coa, and for producing 1,3-butanediol
CA2860416A1 (en) 2011-12-27 2013-07-04 Commonwealth Scientific And Industrial Research Organisation Production of dihydrosterculic acid and derivatives thereof
SG11201406711TA (en) 2012-04-18 2014-11-27 Solazyme Inc Tailored oils
EP3266316A1 (en) 2012-06-15 2018-01-10 Commonwealth Scientific and Industrial Research Organisation Production of long chain polyunsaturated fatty acids in plant cells
US9347075B2 (en) 2012-06-19 2016-05-24 E I Du Pont De Nemours And Company Nucleic acids encoding mutant acyl-CoA:lysophosphatidylcholine acyltransferases
CN102787128A (zh) * 2012-08-28 2012-11-21 江南大学 一种用于多不饱和脂肪酸生物合成的脱饱和酶Δ9 Des
JP2016502851A (ja) 2012-12-21 2016-02-01 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 微生物細胞中における脂質生成を改変するためのｓｏｕ２ソルビトール利用タンパク質をコードするポリヌクレオチドの下方制御
CA2893409A1 (en) 2012-12-21 2014-06-26 E. I. Du Pont De Nemours And Company Recombinant microbial cells that produce at least 28% eicosapentaenoic acid as dry cell weight
KR102271711B1 (ko) 2012-12-24 2021-07-01 퀄리타스 헬스 인코포레이티드 에이코사펜타엔산 (ｅｐａ) 제형
AU2014285720B2 (en) 2013-07-05 2020-10-15 Basf Plant Science Company Gmbh Gene expression or activity enhancing elements
EP3052636A2 (en) 2013-10-04 2016-08-10 Solazyme, Inc. Tailored oils
DE102014220186B4 (de) * 2013-12-12 2017-06-22 Technische Universität Dresden Hefestämme und Verfahren zur Produktion von Omega-Hydroxyfettsäuren und Dicarbonsäuren
WO2015089587A1 (en) 2013-12-18 2015-06-25 Commonwealth Scientific And Industrial Research Organisation Lipid comprising long chain polyunsaturated fatty acids
CN105219789B (zh) 2014-06-27 2023-04-07 联邦科学技术研究组织 包含二十二碳五烯酸的提取的植物脂质
BR112017000414A2 (pt) 2014-07-10 2017-11-07 Terravia Holdings Inc genes cetoacil-acp sintase e utilizações dos mesmos
WO2017015368A1 (en) 2015-07-22 2017-01-26 E I Du Pont De Nemours And Company High level production of long-chain dicarboxylic acids with microbes
CN105181832A (zh) * 2015-08-31 2015-12-23 广州金域医学检验中心有限公司 一种快速检测全血红细胞二十烷二烯酸的方法
BR112017017672A2 (pt) 2015-10-01 2018-07-31 Dsm Ip Assets Bv material suplementar para uso na alimentação de animais domésticos.
US11173136B2 (en) 2018-01-10 2021-11-16 Brightseed, Inc. Method for modulating metabolism
WO2020081637A1 (en) * 2018-10-17 2020-04-23 Archer Daniels Midland Company Enzyme addition to fermentation broth for the reduction of oligosaccharides introduced through sterilized dextrose solution
CN110373436B (zh) * 2018-12-11 2022-09-23 山东理工大学 一种产双高-γ-亚麻酸卷枝毛霉细胞工厂的构建方法及发酵技术
CN110229832A (zh) * 2019-06-16 2019-09-13 山东理工大学 一种提高耶式解脂酵母生物量和细胞壁产量的菌株和方法
CA3148593A1 (en) 2019-07-29 2021-02-04 Brightseed, Inc. Method for improving digestive health
US11572546B2 (en) 2019-07-29 2023-02-07 Ginkgo Bioworks, Inc. Methods and compositions involving promoters derived from Yarrowia lipolytica
MX2022005682A (es) 2019-11-11 2022-08-08 Brightseed Inc Extracto, producto consumible y método para enriquecer metabolito bioactivo en un extracto.
WO2021130078A1 (en) 2019-12-23 2021-07-01 Dsm Ip Assets B.V. Aquaculture feed
WO2022115503A1 (en) 2020-11-25 2022-06-02 Dupont Nutrition Biosciences Aps Treatment and prevention of coronavirus infection
WO2022129592A1 (en) 2020-12-17 2022-06-23 Dsm Ip Assets B.V. Aquaculture feed
WO2023148154A1 (en) 2022-02-01 2023-08-10 Dsm Ip Assets B.V. Aquaculture feed

Family Cites Families (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ190194A (en) 1978-05-08 1982-02-23 Cpc International Inc Production of oil by fermenting yeast in fatty acids
US5071764A (en) * 1983-10-06 1991-12-10 Pfizer Inc. Process for integrative transformation of yarrowia lipolytica
US4880741A (en) * 1983-10-06 1989-11-14 Pfizer Inc. Process for transformation of Yarrowia lipolytica
GB8407195D0 (en) 1984-03-20 1984-04-26 Cadbury Schweppes Plc Microbial desaturase enzyme inhibitors
US4792523A (en) 1984-08-31 1988-12-20 Cetus Corporation 3'Expression enhancing fragments and method
DD227448A1 (de) 1984-10-18 1985-09-18 Akad Wissenschaften Ddr Verfahren zur herstellung von zitronensaeure auf mikrobiellem wege
US4677072A (en) * 1985-02-28 1987-06-30 Westvaco Corporation Rhodotorula having desaturase enzymes
GB8507058D0 (en) 1985-03-19 1985-04-24 Efamol Ltd Pharmaceutical & dietary compositions
GB8524276D0 (en) * 1985-10-02 1985-11-06 Efamol Ltd Pharmaceutical & dietary compositions
GB8524275D0 (en) * 1985-10-02 1985-11-06 Efamol Ltd Pharmaceutical & dietary compositions
US5107065A (en) 1986-03-28 1992-04-21 Calgene, Inc. Anti-sense regulation of gene expression in plant cells
JP2527340B2 (ja) 1986-12-15 1996-08-21 アプライド バイオシステムズ インコーポレイテッド ロ―ダミン染料の５−及び６−スクシニミジルカルボキシレ―ト異性体
DD259637A1 (de) 1987-04-09 1988-08-31 Adw Ddr Verfahren zur herstellung von isozitratlyase in rekombinanten mikroorganismen
SU1454852A1 (ru) 1987-05-19 1989-01-30 Институт Микробиологии Им.Августа Кирхенштейна Штамм дрожжей JaRRoWIa LIроLYтIса-продуцент липазы и питательна среда дл его культивировани
DD267999B5 (de) 1988-01-06 2000-08-10 Akad Wissenschaften Ddr Verfahren zur herstellung von 2-oxoglutarsaeure durch hefen
CA1338683C (en) * 1988-09-13 1996-10-29 Efamol Holdings Plc Fatty acid therapy and compositions
DD275480A1 (de) 1988-09-15 1990-01-24 Ve Forschungszentrum Biotechno Verfahren zur herstellung von zitronensaeure durch aerobe submerse kultivierung von hefen
US5057419A (en) * 1988-09-22 1991-10-15 Rutgers University Genetically engineered plasmid and organisms for the production of specialized oils
DD279267A1 (de) 1989-01-05 1990-05-30 Akad Wissenschaften Ddr Verfahren zur herstellung mikrobieller lipase
PL160027B1 (pl) 1989-03-23 1993-01-29 Akad Rolnicza Sposób jednoczesnego otrzymywania kwasu cytrynowego i kwasu izocytrynowego PL
DD285370A5 (de) 1989-06-29 1990-12-12 Ingenieurtechnik Im Stammbetrieb Veb Kombinat Suesswaren,Dd Verfahren zur immobilisierung von mikroorganismen bzw. coimmobilisierung von mikroorganismen und enzymen
DD285372A5 (de) 1989-06-29 1990-12-12 Ingenieurtechnik,Dd Verfahren zur immobilisierung von mikroorganismen bzw. coimmobilisierung von mikroorganismen und enzymen
US5366860A (en) 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
US5407957A (en) * 1990-02-13 1995-04-18 Martek Corporation Production of docosahexaenoic acid by dinoflagellates
EP0472722B1 (en) 1990-03-16 2003-05-21 Calgene LLC Dnas encoding plant desaturases and their uses
US5244921A (en) * 1990-03-21 1993-09-14 Martek Corporation Eicosapentaenoic acids and methods for their production
ATE110778T1 (de) * 1990-05-25 1994-09-15 Du Pont Nukleotidsequenz des gens für stearoyl-acp- desaturase aus soja.
US5658767A (en) * 1991-01-24 1997-08-19 Martek Corporation Arachidonic acid and methods for the production and use thereof
EP0616644B1 (en) 1991-12-04 2003-07-02 E.I. Du Pont De Nemours And Company Fatty acid desaturase genes from plants
DE69321885T2 (de) 1992-06-25 1999-05-12 Int Flavors & Fragrances Inc Verfahren zur fermentativen Herstellung von 10-Hydroxy-C18-Carbonsäure und Gamma-Dodekalaktonderivaten
IT1256043B (it) 1992-08-12 1995-11-21 Ceppo mutante di yarrowia lipolytica e procedimento per la produzione di acido citrico
DK0668919T3 (da) 1992-11-17 2003-09-15 Du Pont Gener for mikorsomale delta-12-fedtsyredesaturaser og beslægtede enzymer fra planter
FR2734843A1 (fr) 1995-06-02 1996-12-06 Centre Nat Rech Scient Nouveau procede de bioconversion microbienne.
EP0770683A1 (en) 1995-10-04 1997-05-02 Mitsubishi Chemical Corporation Method for producing erythritol
JPH09252790A (ja) 1996-03-21 1997-09-30 Japan Energy Corp 発酵法によるピルビン酸の製造方法
US6166230A (en) * 1996-05-15 2000-12-26 Gist-Brocades B.V. Sterol extraction with polar solvent to give low sterol, high triglyceride, microbial oil
WO1997044470A1 (en) 1996-05-21 1997-11-27 Novo Nordisk A/S Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast
RU2090611C1 (ru) 1996-09-27 1997-09-20 Финогенова Татьяна Васильевна Штамм дрожжей yarrowia lipolytica - продуцент лимонной кислоты, способ получения лимонной кислоты и способ выделения цитрата натрия
US5972664A (en) * 1997-04-11 1999-10-26 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
US6075183A (en) * 1997-04-11 2000-06-13 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids in plants
US5968809A (en) * 1997-04-11 1999-10-19 Abbot Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
JP2002503447A (ja) 1997-12-04 2002-02-05 スミスクライン・ビーチャム・コーポレイション 発現可能なアンチセンス配列を用いる条件発現変異細胞の取得方法
US6403349B1 (en) * 1998-09-02 2002-06-11 Abbott Laboratories Elongase gene and uses thereof
US6677145B2 (en) * 1998-09-02 2004-01-13 Abbott Laboratories Elongase genes and uses thereof
EP0997533A1 (de) 1998-10-24 2000-05-03 Haarmann & Reimer Gmbh Verfahren zur Gewinnung von Gamma-Decalacton
PT1276874E (pt) 2000-04-28 2008-01-11 Mayoly Spindler Lab Clonagem e expressão de uma lipase extracelular resistente aos ácidos de yarrowia lipolytica
CN1380903A (zh) 2000-05-19 2002-11-20 钟渊化学工业株式会社 转化体和用其生成聚酯的方法
BR0205508A (pt) 2001-01-25 2005-05-03 Abbott Lab Genes para dessaturase e usos dos mesmos
JP2002296554A (ja) 2001-03-30 2002-10-09 Minebea Co Ltd ファラデー回転子
US7045683B2 (en) 2001-05-04 2006-05-16 Abbott Laboratories Δ4-desaturase genes and uses thereof
US7211656B2 (en) * 2002-01-30 2007-05-01 Abbott Laboratories Desaturase genes, enzymes encoded thereby, and uses thereof
JP4515904B2 (ja) 2002-05-22 2010-08-04 モンサント テクノロジー エルエルシー 真菌からの脂肪酸不飽和化酵素
US7214491B2 (en) * 2003-05-07 2007-05-08 E. I. Du Pont De Nemours And Company Δ-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts
US7364883B2 (en) * 2004-05-07 2008-04-29 Yeastern Biotech Co., Ltd. Process for producing poly-unsaturated fatty acids by oleaginous yeasts

Also Published As

Publication number Publication date
US7553628B2 (en) 2009-06-30
US20090233347A1 (en) 2009-09-17
EP2402448B1 (en) 2016-02-10
US20130143305A1 (en) 2013-06-06
KR20060018832A (ko) 2006-03-02
CN1816630A (zh) 2006-08-09
CN100591773C (zh) 2010-02-24
EP2392665A3 (en) 2012-04-18
US9133465B2 (en) 2015-09-15
EP2392664B1 (en) 2016-02-10
WO2004101757A2 (en) 2004-11-25
NO20055784L (no) 2006-02-06
CN101824441A (zh) 2010-09-08
EP2392665A2 (en) 2011-12-07
CA2526935A1 (en) 2004-11-25
CN1852986A (zh) 2006-10-25
DK2392664T3 (en) 2016-05-09
DK1620543T3 (en) 2017-03-06
US7238482B2 (en) 2007-07-03
EP1620543A4 (en) 2008-11-19
US20070207528A1 (en) 2007-09-06
EP1620543A2 (en) 2006-02-01
US20050136519A1 (en) 2005-06-23
CA2526935C (en) 2013-12-31
EP2402448A2 (en) 2012-01-04
JP2007504839A (ja) 2007-03-08
US8399242B2 (en) 2013-03-19
EP2402448A3 (en) 2012-04-25
CN1816559A (zh) 2006-08-09
EP2392664A2 (en) 2011-12-07
BRPI0410688A (pt) 2006-06-20
NO20055784D0 (no) 2005-12-06
EP2392664A3 (en) 2012-04-04
WO2004101757A3 (en) 2006-04-13
US20090233346A1 (en) 2009-09-17
EP1620543B1 (en) 2016-11-23

Similar Documents

Publication Publication Date Title
DK2402448T3 (en) Preparation of polyunsaturated fatty acids in OIL YEAST
EP1682567B1 (en) Delta12 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous yeast
US7749703B2 (en) Δ12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts
US7335476B2 (en) Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts