FI94429B - Förfarande för bestämning av nukleinsyror samt vid förfarandet användbar reagenskombination och reagensförpackning - Google Patents

Förfarande för bestämning av nukleinsyror samt vid förfarandet användbar reagenskombination och reagensförpackning Download PDF

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FI94429B
FI94429B FI880994A FI880994A FI94429B FI 94429 B FI94429 B FI 94429B FI 880994 A FI880994 A FI 880994A FI 880994 A FI880994 A FI 880994A FI 94429 B FI94429 B FI 94429B
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nucleic acid
target nucleic
probe
primers
affinity pair
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FI880994A
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English (en)
Finnish (fi)
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FI94429C (sv
FI880994A (sv
FI880994A0 (sv
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Hans Erik Soederlund
Arja Marjatta Weckman
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Orion Yhtymae Oy
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Claims (10)

1. Sätt för analys av nukleinsyror genom hybridisering med hjälp av säväl infängningsprober som detektorprober, kännetecknat av att mälnukleinsyrorna kopieras med hjälp av modifierade polymerisations-primers, vilka efter att de införlivats i kopiorna av mälnukleinsyran fungerar som detektorprober, varefter mälnukleinsyrakopiorna isoleras med hjälp av minst en selektiv infängningsprob och pävisas med hjälp av detektorproben.
2. Sätt enligt patentkravet 1, kännetecknat av att man: (a) förser minst en mälnukleinsyraprimer med minst en detekterbar markör eller minst ett specifikt site, vartill minst en detekterbar markör kan fästas; (b) läter nämnda detektorprimer reagera med den enkelsträngade mälnukleinsyran under betingelser lämpliga för en mallberoende polymerisationsreaktion; (c) hybridiserar de enkelsträngade kopiorna av mälnukleinsyran, väri nämnda detektorprimers har införlivats, med en infängningsprob med förmäga att selektivt hybridiseras tili mälnukleinsyran under betingölser lämpliga för en hybridiseringsreaktion; (d) separerar kopiorna av mälnukleinsyran, väri nämnda detektorprimers har införlivats, med hjälp av den selektiva infängningsproben; (e) pävisar närvaron av mälnukleinsyrakopiorna med hjälp av lämpliga metoder. •
3. Sätt enligt patentkraven 1 och 2, kännetecknad av att den selektiva infängningsproben är försedd med minst en komponent av ett lämpligt affinitetspar eller med minst ett spesifikt site, vartill en komponent av affinitetsparet kan fästas.
4. Sätt för analys av nukleinsyror genom hybridisering med hjälp av säväl infängningsprober som detektorprober, kännetecknat av att mälnukleinsyrorna kopieras med hjälp av modifierade polymerisations-primers, vilka efter att de införlivats i kopiorna av mälnuklelrisyran fungerar som infängningsprober, varefter mälnukleinsyrakopiorna isoleras med hjälp av nämnda infängningsprob och pävisas med hjälp av minst en selektiv detektorprob. 94429
5. Sätt enligt patentkravet 1, kännetecknat av att man: (a) förser minst en mälnukleinsyraprimer med minst en komponent av ett affinitetspar eller minst ett specifikt site vartill minst en komponent av ett affinitetspar kan fästas, (b) läter nämnda infängninsprimer reagera med den enkelsträngade mälnukleinsyran under betingelser lämpliga för en mallberoende polymerisationsreaktion; (c) hybridiserar de enkelsträngade kopiorna av mälnukleinsyran, väri nämnda infängningsprimers har införlivats, med en detektorprob med förmäga att selektivt hybridiseras tili mälnukleinsyran under betingelser lämpliga för en hybridiseringsreaktion; (d) separerar kopiorna av mälnukleinsyran, väri ifrägavarande infängninsprimers har införlivats, med hjälp av den andra komponenten av affinitetsparet; (e) pävisar närvaron av mälnukleinsyrakopioma med hjälp av lämpliga metoder.
6. Sätt enligt patentkraven 1 och 2, kännetecknad av att den selektiva detektorproben är försedd med minst en lämplig markör eller med minst en spesifik site, vartill minst en detekterbar markör kan fästas.
7. En reagenskombination för analys av nukleinsyror, kännetecknad av att den innefattar: * (-/ (a) minst en modifierad polymerisationsprimer, som är specifik för mälnukleinsyran, försedd med minst en detekterbar markör eller minst ett specifikt site, vartill minst en detekterbar markör kan fästas; och (b) minst en selektiv infängningsprob, som är specifik för mälnukleinsyran, försedd med minst en komponent av ett affinitetspar eller minst ett specifikt site, vartill minst en komponent av ett affinitetspar kan fästas.
8. En reagenskombination för analys av nukleinsyror, kännetecknad av att den innefattar: (a) minst en modifierad polymerisationsprimer, som är specifik för mälnukleinsyran, försedd med minst en komponent av ett affinitetspar eller minst ett specifikt site, vartill minst en komponent av ett affinitetspar kan fästas; och 94429 (b) minst en selektiv detektorprob som är specifik för mälnukleinsyran, försedd med minst en detekterbar markör eller minst ett specifikt site vartill minst en detekterbar markör kan fästas.
9. En reagensförpackning för analys av nukleinsyror, kännetecknad av att den bestär av en multibehällarenhet med: (a) minst en modifierad primer försedd med minst en detekterbar markör eller minst ett specifikt site, vartill minst en detekterbar markör kan fästas; (b) minst en selektiv infängningsprob försedd med minst en komponent av ett affinitetspar eller minst ett specifikt site, vartill minst en komponent av ett affinitetspar kan fästas; (c) eventuellt en behällare innefattande minst ett mallberoende polymerisationsreagens; (d) eventuellt en behällare med de fyra deoxynukleosidtrifosfaterna; (e) eventuellt ett hjälpmedel för polymerisations- och hybridiseringsförfarandet; (f) eventuellt ett hjälpmedel för separation av kopiorna av mälnukleinsyran; (g) eventuellt ett hjälpmedel för analys av markören.
10. En reagensförpackning för analys av nukleinsyror, kännetecknad av att den bestär av en multibehällarenhet med: (a) minst en modifierad primer, försedd med minst en komponent av ett affinitetspar eller minst ett specifikt site, vartill minst en komponent av ett affinitetspar kan fästas; (b) minst en selektiv detektorprob försedd med minst en detekterbar markör eller minst ett specifikt site, vartill minst en detekterbar markör kan fästas; (c) eventuellt en behällare innefattande minst ett mallbäroende polymerisationsreagens; (d) eventuellt en behällare med de fyra deoxynukleosidtrifosfaterna; (e) eventuellt ett hjälpmedel för polymerisations- och hybridiseringsförfarandet; (f) eventuellt ett hjälpmedel för separation av kopiorna av mälnukleinsyran; (g) eventuellt ett hjälpmedel för analys av markören. «I - ii-filf J
FI880994A 1987-03-11 1988-03-03 Förfarande för bestämning av nukleinsyror samt vid förfarandet användbar reagenskombination och reagensförpackning FI94429C (sv)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US2460487A 1987-03-11 1987-03-11
US2460487 1987-03-11

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FI880994A0 FI880994A0 (sv) 1988-03-03
FI880994A FI880994A (sv) 1988-09-12
FI94429B true FI94429B (sv) 1995-05-31
FI94429C FI94429C (sv) 1995-09-11

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US (2) US5476769A (sv)
JP (1) JP3244500B2 (sv)
AT (1) AT395434B (sv)
AU (1) AU622104B2 (sv)
BE (1) BE1003990A5 (sv)
CA (1) CA1338207C (sv)
CH (1) CH677285A5 (sv)
DD (1) DD272664A5 (sv)
DE (1) DE3807994C2 (sv)
DK (1) DK175384B1 (sv)
ES (1) ES2006377A6 (sv)
FI (1) FI94429C (sv)
FR (1) FR2613077A1 (sv)
GB (1) GB2202328B (sv)
GR (1) GR1000315B (sv)
HU (1) HU202583B (sv)
IE (1) IE61664B1 (sv)
IL (3) IL85480A0 (sv)
IT (1) IT1216048B (sv)
LU (1) LU87153A1 (sv)
NL (1) NL194922C (sv)
NO (1) NO172909C (sv)
NZ (1) NZ223700A (sv)
PT (1) PT86937B (sv)
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Families Citing this family (171)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5753433A (en) * 1909-12-05 1998-05-19 Boehringer Mannheim Gmbh Method for the sensitive detection of nucleic acids
DE4038804A1 (de) 1990-10-09 1992-04-16 Boehringer Mannheim Gmbh Verfahren zur genus- oder/und spezies-spezifischen detektion von bakterien in einer probenfluessigkeit
US5714380A (en) 1986-10-23 1998-02-03 Amoco Corporation Closed vessel for isolating target molecules and for performing amplification
FI80476C (sv) * 1987-10-09 1990-06-11 Orion Yhtymae Oy Förbättrat hybridisationsförfarande, vid förfarandet använt redskap oc h reagensförpackning
CA1325761C (en) * 1987-12-25 1994-01-04 Takanori Oka Method of detecting an intended nucleic acid in a sample
JPH0775560B2 (ja) * 1987-12-25 1995-08-16 湧永製薬株式会社 検体中の目的核酸の検出法
WO1989009281A1 (en) * 1988-03-25 1989-10-05 Akzo N.V. Method for amplifying and detecting nucleic acid in a test liquid
US5817797A (en) * 1988-06-01 1998-10-06 The United States Of America As Represented By The Department Of Health And Human Services Sequencing DNA; a modification of the polymerase chain reaction
US5858652A (en) * 1988-08-30 1999-01-12 Abbott Laboratories Detection and amplification of target nucleic acid sequences
AU629845B2 (en) * 1988-08-30 1992-10-15 Abbott Laboratories Detection and amplification of target nucleic acid sequences
CA2002076A1 (en) * 1988-11-21 1990-05-21 Brent A. Burdick Diagnostic kit and method using a solid phase capture means for detecting nucleic acids
GB8827160D0 (en) * 1988-11-21 1988-12-29 Apothekernes Lab Detection & quantitative determination of rna & dna
IL92474A (en) * 1988-11-29 1994-04-12 Orion Yhtymae Oy Reaction method and combination for determining nucleotide sequences
US5536648A (en) * 1988-12-09 1996-07-16 Amrad Corporation Limited Amplified DNA assay using a double stranded DNA binding protein
EP0447464B1 (en) * 1988-12-09 1998-08-05 Amrad Corporation Limited Amplified dna assay
CA2004326A1 (en) * 1988-12-23 1990-06-23 Nanibushan Dattagupta Assay of sequences using amplified genes
CA2008096A1 (en) * 1989-01-19 1990-07-19 Samuel Rose Nucleic acid amplification using single primer
US5856092A (en) * 1989-02-13 1999-01-05 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
ES2136059T5 (es) * 1989-02-13 2005-02-16 Geneco Pty. Ltd. Deteccion de una secuencia de acidos nucleicos o de un cambio en la misma.
CA2011818A1 (en) * 1989-03-17 1990-09-17 Fred T. Oakes Methods for purification, amplification and detection of a nucleic acid
CA2013317A1 (en) * 1989-04-17 1990-10-17 Fred T. Oakes Diagnostic kit, primer composition and their use for replication or detection of nucleic acids
DE69003104T2 (de) * 1989-06-12 1994-03-17 Cis Bio International Saclay Verfahren zum nachweis von spezifischen sequenzen von nukleinsäuren und ihre verwendungen.
GB8920097D0 (en) * 1989-09-06 1989-10-18 Ici Plc Amplification processes
US5196305A (en) * 1989-09-12 1993-03-23 Eastman Kodak Company Diagnostic and amplification methods using primers having thymine at 3' end to overcome primer-target mismatch at the 3' end
US5232829A (en) * 1989-09-29 1993-08-03 Hoffmann-La Roche Inc. Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes
IL97222A (en) * 1990-02-16 1995-08-31 Orion Yhtymae Oy Method and Responder for Determining Special Changes to Nucleotide
US6013431A (en) 1990-02-16 2000-01-11 Molecular Tool, Inc. Method for determining specific nucleotide variations by primer extension in the presence of mixture of labeled nucleotides and terminators
US5387505A (en) * 1990-05-04 1995-02-07 Eastman Kodak Company Preparation and isolation of single-stranded biotinylated nucleic acids by heat avidin-biotin cleavage
NZ240079A (en) * 1990-10-09 1993-07-27 Boehringer Mannheim Gmbh Method for the detection of a nucleic acid or part thereof
WO1992011390A1 (en) * 1990-12-17 1992-07-09 Idexx Laboratories, Inc. Nucleic acid sequence detection by triple helix formation
DE4106251A1 (de) * 1991-02-28 1992-09-03 Boehringer Mannheim Gmbh Nachweis von bakterien mit hilfe einer nukleinsaeureamplifikation
US5888819A (en) * 1991-03-05 1999-03-30 Molecular Tool, Inc. Method for determining nucleotide identity through primer extension
US6004744A (en) 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
CA2065719A1 (en) * 1991-04-30 1992-10-31 John B. Findlay Nucleic acid amplification and detection methods using rapid polymerase chain reaction cycle
US5387510A (en) * 1991-10-02 1995-02-07 Eastman Kodak Company Detection of amplified nucleic acid using secondary capture oligonucleotides and test kit
IT1252295B (it) * 1991-11-15 1995-06-08 Schiapparelli Diagnostici Ismu Metodo per rivelare acidi nucleici e relativo kit diagnostico
JPH07502655A (ja) * 1991-12-23 1995-03-23 バイエル　コーポレイション 溶液相サンドイッチハイブリダイゼーションアッセイに用いるためのｃｍｖプローブ
US5863724A (en) * 1994-04-13 1999-01-26 Baylor College Of Medicine Methods of screening for persistent hyperinsulinemic hypoglycemia of infancy
US5658729A (en) * 1994-10-11 1997-08-19 The University Of British Columbia Method, reagent and kit for evaluating susceptibility to premature atherosclerosis
DE4421901A1 (de) * 1994-06-23 1996-01-04 Bayer Ag Ein DNA-Schnelltest zum Nachweis von chinolonresistenten Staphylococcus aureus Erregern in klinischem Probenmaterial
US5705366A (en) * 1994-09-15 1998-01-06 Johnson & Johnson Clinical Diagnostics, Inc. Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor
US5654141A (en) * 1994-11-18 1997-08-05 Thomas Jefferson University Amplification based detection of bacterial infection
US5856096A (en) * 1995-09-20 1999-01-05 Ctrc Research Foundation Rapid and sensitive assays for detecting and distinguishing between processive and non-processive telomerase activities
WO1997035033A1 (en) 1996-03-19 1997-09-25 Molecular Tool, Inc. Method for determining the nucleotide sequence of a polynucleotide
US6132954A (en) * 1996-08-20 2000-10-17 Baylor College Of Medicine Methods of screening for agents that delay a cell cycle and compositions comprising era and an analogue of wild-type era
US6043283A (en) * 1996-09-20 2000-03-28 Baylor College Of Medicine Tyramine compounds and their neuronal effects
US6071493A (en) 1996-09-20 2000-06-06 Baylor College Of Medicine Method of screening for an agent that inhibits mononuclear phagocyte-plaque component complex formation
US6482795B1 (en) 1997-01-30 2002-11-19 Myriad Genetics, Inc. Tumor suppressor designated TS10q23.3
US6262242B1 (en) 1997-01-30 2001-07-17 Board Of Regents, The University Of Texas System Tumor suppressor designated TS10Q23.3
US6713300B1 (en) 1997-02-27 2004-03-30 University Of Utah Research Foundation Nucleic acid and amino acid sequences for ATP-binding cassette transporter and methods of screening for agents that modify ATP-binding cassette transporter
IL122147A0 (en) * 1997-11-10 1998-04-05 Technion Res & Dev Foundation Method for labeling polynucleotides
EP1040192B1 (en) 1997-12-18 2006-08-09 Monsanto Technology LLC Insect-resistant transgenic plants and methods for improving delta-endotoxin activity against insects
US6673914B1 (en) 1998-01-22 2004-01-06 John Wayne Cancer Institute Human tumor-associated gene
US20020147143A1 (en) 1998-03-18 2002-10-10 Corixa Corporation Compositions and methods for the therapy and diagnosis of lung cancer
DE19824900B4 (de) 1998-06-04 2006-05-04 Roche Diagnostics Gmbh DNA-Nachweis über einen Strangreassoziationskomplex
US6743906B1 (en) 1998-10-02 2004-06-01 Board Of Regents, The University Of Texas PPP2R1B is a tumor suppressor
US6468523B1 (en) 1998-11-02 2002-10-22 Monsanto Technology Llc Polypeptide compositions toxic to diabrotic insects, and methods of use
WO2000029008A2 (en) 1998-11-16 2000-05-25 Board Of Regents, The University Of Texas System Hiv-specific t-cell induction
US6432642B1 (en) 1999-01-15 2002-08-13 Pe Corporation (Ny) Binary probe and clamp composition and methods for a target hybridization detection
US6156515A (en) * 1999-02-09 2000-12-05 Urocor, Inc. Prostate-specific gene for diagnosis, prognosis and management of prostate cancer
JP2002542805A (ja) 1999-04-30 2002-12-17 ユニバーシティ オブ フロリダ アデノ随伴ウイルス送達リボザイム組成物および使用方法
DE60045350D1 (de) 1999-06-01 2011-01-20 Baylor College Medicine Zusammensetzungen und verfahren zur therapeutischen anwendung einer mit dem gen atonal assoziierten sequenz
US20040002068A1 (en) 2000-03-01 2004-01-01 Corixa Corporation Compositions and methods for the detection, diagnosis and therapy of hematological malignancies
EP1278855B1 (en) 2000-04-21 2008-03-12 Corixa Corporation Compounds and methods for treatment and diagnosis of chlamydial infection
ATE396265T1 (de) 2000-06-28 2008-06-15 Corixa Corp Zusammensetzungen und verfahren für therapie und diagnose von lungenkrebs
WO2002004511A2 (en) 2000-07-10 2002-01-17 Board Of Regents, The University Of Texas System CHROMOSOME 3p21.3 GENES ARE TUMOR SUPPRESSORS
JP2005504513A (ja) 2001-05-09 2005-02-17 コリクサ コーポレイション 前立腺癌の治療及び診断のための組成物及び方法
EP1399541A4 (en) 2001-05-22 2005-04-13 Univ Chicago RNA POLYMERASE DEPENDENT ON SINGLE VIBRATION N4 DNA
EP1908851A3 (en) 2001-09-19 2008-06-25 Intergenetics Incorporated Genetic analysis for stratification of cancer risk
WO2003025141A2 (en) 2001-09-19 2003-03-27 Intergenetics Incorporated Genetic analysis for stratification of cancer risk
ES2205998B1 (es) * 2001-12-14 2005-08-16 Consejo Sup. Investig. Cientificas. Procedimiento para la deteccion del virus del amarilleo de las venas del pepino (cvyv).
AU2002365279B2 (en) 2001-12-17 2009-08-13 Corixa Corporation Compositions and methods for the therapy and diagnosis of inflammatory bowel disease
EP2221377B2 (en) 2002-02-01 2017-05-17 Life Technologies Corporation Oligonucleotide compositions with enhanced efficiency
US20060009409A1 (en) 2002-02-01 2006-01-12 Woolf Tod M Double-stranded oligonucleotides
US6916474B2 (en) 2002-07-15 2005-07-12 Board Of Regents, The University Of Texas System Antibodies with increased affinities for anthrax antigens
EP2269619A1 (en) 2002-08-12 2011-01-05 Jennerex Biotherapeutics ULC Methods and compositions concerning poxviruses and cancer
AU2002951411A0 (en) 2002-09-16 2002-09-26 The University Of Sydney Genotype screen
US8349276B2 (en) 2002-09-24 2013-01-08 Duke University Apparatuses and methods for manipulating droplets on a printed circuit board
US7960522B2 (en) 2003-01-06 2011-06-14 Corixa Corporation Certain aminoalkyl glucosaminide phosphate compounds and their use
MXPA05007295A (es) 2003-01-06 2005-09-30 Corixa Corp Ciertos compuestos de fosfato de aminoalquil glucosaminida y su uso.
WO2004081225A2 (en) 2003-03-07 2004-09-23 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a dna polymerization process
US20050059024A1 (en) 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
ATE554166T1 (de) 2003-07-25 2012-05-15 Life Technologies Corp Verfahren und zusammensetzungen zur herstellung von rna aus einer fixierten probe
EP2527446A1 (en) 2004-06-03 2012-11-28 Athlomics Pty Ltd Agents and methods for diagnosing stress
WO2006017724A1 (en) * 2004-08-06 2006-02-16 Becton, Dickinson And Company Sequences and methods for detection of cytomegalovirus
CA2581086C (en) 2004-09-14 2023-11-07 The Regents Of The University Of Colorado, A Body Corporate Method for treatment with bucindolol based on genetic targeting
ATE551433T1 (de) 2005-05-06 2012-04-15 Gen Probe Inc Verfahren und produkte zum erfassen von nukleinsäurezielen
EP2476761A3 (en) 2005-07-07 2012-10-17 Athlomics Pty Ltd Polynucleotide marker genes and their expression, for diagnosis of endotoxemia
US7494788B2 (en) 2005-07-11 2009-02-24 Molecular Kinetics, Inc. Entropic bristle domain sequences and their use in recombinant protein production
US8980246B2 (en) 2005-09-07 2015-03-17 Sillajen Biotherapeutics, Inc. Oncolytic vaccinia virus cancer therapy
US8239136B2 (en) 2005-10-21 2012-08-07 Genenews Inc. Method, computer system and computer-readable medium for determining a probability of colorectal cancer in a test subject
US20090317421A1 (en) 2006-01-18 2009-12-24 Dominique Missiakas Compositions and methods related to staphylococcal bacterium proteins
WO2007106579A2 (en) 2006-03-15 2007-09-20 Micronics, Inc. Integrated nucleic acid assays
CN102026645B (zh) 2006-09-15 2016-01-27 渥太华医院研究机构 溶瘤弹状病毒
DK2102239T3 (da) 2006-11-30 2012-05-29 Res Dev Foundation Forbedrede immunoglobulin-biblioteker
US9938641B2 (en) * 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
PL2191001T3 (pl) 2007-04-09 2017-01-31 University Of Florida Research Foundation, Inc. Kompozycje wektora RAAV mające białka kapsydu zmodyfikowane tyrozyną i sposoby ich zastosowania
DK2155789T3 (da) 2007-05-01 2013-10-21 Res Dev Foundation Immunoglobulin-Fc-biblioteker
EP2341929B1 (en) 2008-10-06 2017-01-25 University Of Chicago Compositions and methods related to bacterial emp proteins
HUE057713T2 (hu) 2009-04-03 2022-05-28 Univ Chicago A protein A (SPA) variánsaival kapcsolatos készítmények és módszerek
CA2759611C (en) 2009-04-22 2018-11-13 Indiana University Research And Technology Corporation Compositions and methods for the treatment of chronic obstructive pulmonary disease and asthma
US9587270B2 (en) 2009-06-29 2017-03-07 Luminex Corporation Chimeric primers with hairpin conformations and methods of using same
EP2272979A1 (en) 2009-06-30 2011-01-12 Centre National de la Recherche Scientifique (CNRS) Method for testing a subject thought to be predisposed to having cancer
EP3636759B1 (en) 2009-07-17 2023-10-11 BioAtla, Inc. Simultaneous, integrated selection and evolution of antibody/protein performance and expression in production hosts
US9409983B2 (en) 2009-07-23 2016-08-09 The Board Of Trustess Of The University Of Illinois Methods and compositions involving PBEF inhibitors for lung inflammation conditions and diseases
WO2011032088A1 (en) 2009-09-11 2011-03-17 Arca Biopharma, Inc. Polymorphisms in the pde3a gene
ES2848650T3 (es) 2009-09-14 2021-08-11 Sillajen Biotherapeutics Inc Terapia combinada contra el cáncer con virus vaccinia oncolítico
AU2010329551B2 (en) 2009-12-10 2016-02-11 Turnstone Limited Partnership Oncolytic rhabdovirus
DK2515899T3 (en) 2009-12-23 2016-08-15 Arca Biopharma Inc METHODS AND COMPOSITIONS FOR CARDIOVASCULAR DISEASES AND CONDITIONS
CA2786569C (en) 2010-01-29 2019-04-09 Micronics, Inc. Sample-to-answer microfluidic cartridge
JP2013523818A (ja) 2010-04-05 2013-06-17 ザ・ユニバーシティー・オブ・シカゴ 免疫反応のエンハンサーとしてのプロテインＡ（ＳｐＡ）抗体に関連する組成物および方法
LT2561067T (lt) 2010-04-23 2019-03-12 University Of Florida Research Foundation, Inc. Raav-guanilato ciklazės kompozicijos ir būdai, skirti leberio įgimtosios amaurozės-1 (lca1) gydymui
WO2012003474A2 (en) 2010-07-02 2012-01-05 The University Of Chicago Compositions and methods related to protein a (spa) variants
EP2593797B1 (en) 2010-07-16 2019-05-08 Bioatla, LLC Novel methods of protein evolution
US9095540B2 (en) 2010-09-09 2015-08-04 The University Of Chicago Methods and compositions involving protective staphylococcal antigens
EP2455485A1 (de) 2010-11-19 2012-05-23 Anagnostics Bioanalysis GmbH Verfahren zum Nachweis von Nukleinsäuren
CN103429258B (zh) 2011-01-04 2016-03-09 新罗杰公司 通过施用溶瘤痘苗病毒生成针对肿瘤抗原的抗体和生成肿瘤特异性补体依赖性细胞毒性
AU2012214643B2 (en) 2011-02-07 2016-12-15 Research Development Foundation Engineered immunoglobulin Fc polypeptides
CA2828532A1 (en) 2011-02-28 2012-11-22 University Of Iowa Research Foundation Anti-mullerian hormone changes in pregnancy and prediction of adverse pregnancy outcomes and gender
AU2012226530B2 (en) 2011-03-08 2016-12-01 King Abdullah University Of Science And Technology Molecular biomarker set for early detection of ovarian cancer
US9085631B2 (en) 2011-04-08 2015-07-21 Nov Vac APS Proteins and nucleic acids useful in vaccines targeting Staphylococcus aureus
US8945588B2 (en) 2011-05-06 2015-02-03 The University Of Chicago Methods and compositions involving protective staphylococcal antigens, such as EBH polypeptides
WO2012167382A1 (en) 2011-06-08 2012-12-13 Children's Hospital Of Eastern Ontario Research Institute Inc. Compositions and methods for glioblastoma treatment
MX2013013627A (es) 2011-06-21 2014-04-25 Oncofactor Corp Composiciones y metodos para la terapia y diagnostico de cancer.
US10040853B2 (en) 2011-09-09 2018-08-07 Fred Hutchinson Cancer Research Center Methods and compositions involving NKG2D inhibitors and cancer
US9273102B2 (en) 2011-10-12 2016-03-01 Niels Iversen Møller Peptides derived from Campylobacter jejuni and their use in vaccination
US20140349858A1 (en) 2011-12-22 2014-11-27 Ibis Bioscience, Inc. Amplification of a sequence from a ribonucleic acid
US9701959B2 (en) 2012-02-02 2017-07-11 Invenra Inc. High throughput screen for biologically active polypeptides
WO2013162746A1 (en) 2012-04-26 2013-10-31 University Of Chicago Staphylococcal coagulase antigens and methods of their use
US9416403B2 (en) * 2012-08-31 2016-08-16 Toray Industries, Inc. Method of detecting target nucleic acid
ES2730690T3 (es) 2012-12-07 2019-11-12 Suppremol Gmbh Estratificación y tratamiento de pacientes que padecen púrpura trombocitopénica idiopática
EP3549674B1 (en) 2012-12-21 2020-08-12 PerkinElmer Health Sciences, Inc. Low elasticity films for microfluidic use
JP6498125B2 (ja) 2012-12-21 2019-04-10 マイクロニクス， インコーポレイテッド 流体回路および関連する製造方法
KR20150097764A (ko) 2012-12-21 2015-08-26 마이크로닉스 인코포레이티드. 휴대형 형광 검출 시스템 및 미량분석 카트리지
US10125373B2 (en) 2013-01-22 2018-11-13 Arizona Board Of Regents On Behalf Of Arizona State University Geminiviral vector for expression of rituximab
RU2684211C2 (ru) 2013-02-21 2019-04-04 Тёрнстоун Лимитед Партнершип Композиция вакцины
US10386377B2 (en) 2013-05-07 2019-08-20 Micronics, Inc. Microfluidic devices and methods for performing serum separation and blood cross-matching
CA2911308C (en) 2013-05-07 2021-10-19 Micronics, Inc. Device for preparation and analysis of nucleic acids
EP2994532B1 (en) 2013-05-07 2017-11-15 Micronics, Inc. Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions
WO2015051110A1 (en) 2013-10-03 2015-04-09 Oklahoma Medical Research Foundation Biomarkers for systemic lupus erythematosus disease activity, and intensity and flare
EP4227685A3 (en) 2013-12-03 2024-02-28 Evaxion Biotech A/S Proteins and nucleic acids useful in vaccines targeting staphylococcus aureus
WO2015105888A1 (en) 2014-01-07 2015-07-16 Bioatla, Llc Proteins targeting orthologs
KR20170016915A (ko) 2014-06-11 2017-02-14 마이크로닉스 인코포레이티드. 핵산의 분석을 위한 통합 검정 대조군을 갖는 마이크로 유체 공학적 카트리지 및 장치
US20180169211A1 (en) 2014-11-13 2018-06-21 Evaxion Biotech Aps Peptides derived from acinetobacter baumannii and their use in vaccination
AU2015360694B2 (en) 2014-12-08 2021-10-14 Berg Llc Use of markers including filamin a in the diagnosis and treatment of prostate cancer
US10434162B2 (en) 2015-01-12 2019-10-08 Evaxion Biotech Aps Proteins and nucleic acids useful in vaccines targeting Klebsiella pneumoniae
JP6724023B2 (ja) 2015-02-09 2020-07-15 リサーチ ディベロップメント ファウンデーション 改善された補体活性化を示す操作された免疫グロブリンｆｃポリペプチド
EP3070178B1 (en) 2015-03-20 2020-02-12 Albert-Ludwigs-Universität Freiburg Method for diagnosing breast cancer
WO2017005670A1 (en) 2015-07-04 2017-01-12 Evaxion Biotech Aps Proteins and nucleic acids useful in vaccines targeting pseudomonas aeruginosa
CN108026575B (zh) 2015-07-17 2022-08-19 哈佛学院董事及会员团体 扩增核酸序列的方法
WO2017040380A2 (en) 2015-08-28 2017-03-09 Research Development Foundation Engineered antibody fc variants
WO2017144523A1 (en) 2016-02-22 2017-08-31 Evaxion Biotech Aps Proteins and nucleic acids useful in vaccines targeting staphylococcus aureus
EP3465200A4 (en) 2016-06-05 2020-07-08 Berg LLC PATIENT STRATIFICATION SYSTEMS AND METHODS AND IDENTIFICATION OF POTENTIAL BIOMARKERS
US11718648B2 (en) 2017-01-05 2023-08-08 Evaxion Biotech A/S Vaccines targeting Pseudomonas aeruginosa
US20190390278A1 (en) 2017-01-26 2019-12-26 Oklahoma Medical Research Foundation Biomarkers for systemic lupus erythematosus disease activity, and intensity and flare
KR102554477B1 (ko) 2018-10-18 2023-07-11 오클라호마 메디컬 리써치 화운데이션 질병 활성도의 특징을 규명하는 전신 홍반성 낭창(sle) 질병 활성도 면역 지수를 위한 생체지표
WO2020083904A1 (en) 2018-10-22 2020-04-30 Evaxion Biotech Aps Vaccines targeting m. catharrhalis
WO2020174044A1 (en) 2019-02-27 2020-09-03 Evaxion Biotech Aps Vaccines targeting h. influenzae
AU2020265250A1 (en) 2019-04-30 2021-11-18 Larimar Therapeutics, Inc. Frataxin-sensitive markers for determining effectiveness of frataxin replacement therapy
ES2960366T3 (es) 2019-08-09 2024-03-04 Univ Freiburg Albert Ludwigs Método para diagnosticar cáncer de mama
EP4087593A1 (en) 2020-01-06 2022-11-16 Evaxion Biotech A/S Vaccines targeting neisseria gonorrhoeae
EP4172370A1 (en) 2020-06-30 2023-05-03 Lunglife Ai, Inc. Methods for detecting lung cancer
WO2022047359A1 (en) 2020-08-31 2022-03-03 Berg Llc Protein biomarkers for pancreatic cancer
IL307530A (en) 2021-04-06 2023-12-01 Bpgbio Inc Estrogen receptor-like (ER) breast cancer protein markers LUMINAL A (LA) and LUMINAL B1 (LB1)
WO2022216846A1 (en) 2021-04-06 2022-10-13 Berg Llc Protein markers for estrogen receptor (er)-positive-like and estrogen receptor (er)-negative-like breast cancer
CA3214833A1 (en) 2021-04-06 2022-10-13 Bpgbio, Inc. Protein markers for the prognosis of breast cancer progression
CA3224564A1 (en) 2021-07-05 2023-01-12 Andreas Holm MATTSSON Vaccines targeting neisseria gonorrhoeae
US20230357851A1 (en) 2022-04-06 2023-11-09 Larimar Therapeutics, Inc. Frataxin-sensitive markers for monitoring frataxin-replacement therapy
WO2023230531A1 (en) 2022-05-24 2023-11-30 Lunglife Ai, Inc. Methods for detecting circulating genetically abnormal cells
WO2023240201A1 (en) 2022-06-08 2023-12-14 Larimar Therapeutics, Inc. Frataxin-sensitive markers for monitoring progression and treatment of leigh syndrome
US20240010742A1 (en) 2022-06-10 2024-01-11 Research Development Foundation Engineered fcriib selective igg1 fc variants and uses thereof

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2422956A1 (fr) * 1978-04-13 1979-11-09 Pasteur Institut Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede
US4302204A (en) * 1979-07-02 1981-11-24 The Board Of Trustees Of Leland Stanford Junior University Transfer and detection of nucleic acids
FI63596C (fi) * 1981-10-16 1983-07-11 Orion Yhtymae Oy Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser
BG36086A1 (en) * 1982-01-19 1984-09-14 Glbov Method for inducing interferon
CA1223831A (en) * 1982-06-23 1987-07-07 Dean Engelhardt Modified nucleotides, methods of preparing and utilizing and compositions containing the same
US4605735A (en) * 1983-02-14 1986-08-12 Wakunaga Seiyaku Kabushiki Kaisha Oligonucleotide derivatives
NO843838L (no) * 1983-09-26 1985-03-27 Ortho Diagnostic Systems Inc Fremgangsmaate for detektering av nukleinsyre
US4749647A (en) * 1984-06-22 1988-06-07 Genetic Systems Corporation Polymerization-induced separation assay using recognition pairs
US4743562A (en) * 1984-08-21 1988-05-10 The Board Of Trustees Of The Leland Stanford Junior University Purified human cytomegalovirus protein
US4563417A (en) * 1984-08-31 1986-01-07 Miles Laboratories, Inc. Nucleic acid hybridization assay employing antibodies to intercalation complexes
US4883750A (en) * 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
FI72146C (sv) * 1985-01-02 1987-04-13 Orion Yhtymae Oy Förfarande för identifiering av nukleinsyror.
CA1272443A (en) * 1985-02-22 1990-08-07 Nanibhushan Dattagupta Solution-phase dual hybridization assay for detecting polynucleotide sequences
CA1278755C (en) * 1985-03-15 1991-01-08 William J. Knowles Labelling of oligonucleotides
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
DK171161B1 (da) * 1985-03-28 1996-07-08 Hoffmann La Roche Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve
GB8509367D0 (en) * 1985-04-12 1985-05-15 Amersham Int Plc Nucleic acid hybridisation
US4751177A (en) * 1985-06-13 1988-06-14 Amgen Methods and kits for performing nucleic acid hybridization assays
GB8515276D0 (en) * 1985-06-17 1985-07-17 Amersham Int Plc Nucleic acid sequencing
CA1284931C (en) * 1986-03-13 1991-06-18 Henry A. Erlich Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
EP0238332A2 (en) * 1986-03-19 1987-09-23 Cetus Corporation Liquid hybridization method and kit for detecting the presence of nucleic acid sequences in samples
US4851331A (en) * 1986-05-16 1989-07-25 Allied Corporation Method and kit for polynucleotide assay including primer-dependant DNA polymerase

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HU202583B (en) 1991-03-28
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