ES8608579A1 - Procedimiento para introducir dna en un anfitrion de cepa de microorganismo unicelular - Google Patents

Procedimiento para introducir dna en un anfitrion de cepa de microorganismo unicelular

Info

Publication number
ES8608579A1
ES8608579A1 ES534102A ES534102A ES8608579A1 ES 8608579 A1 ES8608579 A1 ES 8608579A1 ES 534102 A ES534102 A ES 534102A ES 534102 A ES534102 A ES 534102A ES 8608579 A1 ES8608579 A1 ES 8608579A1
Authority
ES
Spain
Prior art keywords
industrial
strains
expression
interest
molecular cloning
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES534102A
Other languages
English (en)
Spanish (es)
Other versions
ES534102A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gist Brocades NV
Original Assignee
Gist Brocades NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8190976&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES8608579(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Gist Brocades NV filed Critical Gist Brocades NV
Publication of ES8608579A1 publication Critical patent/ES8608579A1/es
Publication of ES534102A0 publication Critical patent/ES534102A0/es
Expired legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • C12N9/2417Alpha-amylase (3.2.1.1.) from microbiological source
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478Aspartic endopeptidases (3.4.23)
    • C12N9/6481Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Compounds Of Unknown Constitution (AREA)
ES534102A 1983-07-06 1984-07-06 Procedimiento para introducir dna en un anfitrion de cepa de microorganismo unicelular Expired ES8608579A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP83201016 1983-07-06

Publications (2)

Publication Number Publication Date
ES8608579A1 true ES8608579A1 (es) 1986-06-16
ES534102A0 ES534102A0 (es) 1986-06-16

Family

ID=8190976

Family Applications (2)

Application Number Title Priority Date Filing Date
ES534102A Expired ES8608579A1 (es) 1983-07-06 1984-07-06 Procedimiento para introducir dna en un anfitrion de cepa de microorganismo unicelular
ES545256A Expired ES8609467A1 (es) 1983-07-06 1985-07-16 Procedimiento para preparar un polipetido de interes en una cepa de bacilus industrial

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES545256A Expired ES8609467A1 (es) 1983-07-06 1985-07-16 Procedimiento para preparar un polipetido de interes en una cepa de bacilus industrial

Country Status (11)

Country Link
EP (1) EP0134048B2 (esLanguage)
JP (2) JP2669457B2 (esLanguage)
AU (1) AU570709B2 (esLanguage)
DE (1) DE3480411D1 (esLanguage)
DK (1) DK169996B1 (esLanguage)
ES (2) ES8608579A1 (esLanguage)
FI (1) FI85287C (esLanguage)
GR (1) GR82200B (esLanguage)
IE (1) IE58014B1 (esLanguage)
NZ (1) NZ208806A (esLanguage)
WO (1) WO1985000382A1 (esLanguage)

Families Citing this family (104)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1311429C (en) * 1983-07-06 1992-12-15 Vasantha Nagarajan Vector for polypeptides in bacilli
US5624829A (en) * 1984-07-03 1997-04-29 Gist-Brocades, B.V. Transformed industrial bacillus strains and methods for making and using them
DE3484346D1 (de) * 1983-12-28 1991-05-02 Agency Ind Science Techn Dns-basensequenz enthaltende regionen einbegriffen in der herstellung und sekretion eines proteins, rekombinante dns das ganze oder einen teil der dns-basensequenz enthaltend und verfahren zur herstellung von proteinen unter verwendung der rekombinanten dns.
JPS60188093A (ja) * 1984-03-09 1985-09-25 Teruhiko Beppu 枯草菌に於ける形質発現プラスミド
IE58099B1 (en) * 1984-06-06 1993-06-30 Cpc International Inc Amylase-negative, asporogenous mutant of bacillus subtilis useful as a host in a host-vector system
US4801537A (en) * 1984-06-08 1989-01-31 Genex Corporation Vector for expression of polypeptides in bacilli
JPH0616711B2 (ja) * 1985-09-27 1994-03-09 メルシャン株式会社 高温で自律増殖するプラスミド
US5024943A (en) * 1985-11-07 1991-06-18 Gist-Brocades Regulatory region cloning and analysis plasmid for bacillus
DK311186D0 (da) * 1986-06-30 1986-06-30 Novo Industri As Enzymer
AU620326B2 (en) 1987-02-27 1992-02-20 Dsm Ip Assets B.V. Stable gene amplification in chromosomal dna of prokaryotic microorganisms
ES2135386T3 (es) * 1987-02-27 1999-11-01 Genencor Int Transformacion de cepas de bacillus alcalofilas.
BR8805647A (pt) * 1987-02-27 1989-10-31 Gist Brocades Nv Processo para clonagem molecular e expressao de genes que codificam para enzimas proteoliticas
BR9006818A (pt) * 1989-06-29 1991-08-06 Gist Brocades Nv Amilases microbianas mutantes com maior estabilidade termica,acida e/ou alcalina
DE4216246A1 (de) * 1992-05-16 1993-11-18 Solvay Enzymes Gmbh & Co Kg Einfaches Verfahren zur stabilen chromosomalen Genamplifikation
GB9907461D0 (en) 1999-03-31 1999-05-26 King S College London Neurite regeneration
US20050079573A1 (en) 1998-12-23 2005-04-14 Danisco A/S Proteins
GB0020498D0 (en) 2000-08-18 2000-10-11 Sterix Ltd Compound
CZ2003798A3 (cs) 2000-09-21 2003-08-13 Dsm N. V. Polypeptid s aktivitou xylanázy, sekvence nukleové kyseliny kódující polypeptid, způsob přípravy a použití polypeptidu
JP4443829B2 (ja) 2000-12-07 2010-03-31 ディーエスエム アイピー アセッツ ビー.ブイ. 飲料中の濁りを防止または減少する方法
CN100506981C (zh) 2000-12-07 2009-07-01 Dsmip资产有限公司 富含具有羧基末端脯氨酸残基的肽的蛋白质水解产物
GB0116453D0 (en) 2001-07-05 2001-08-29 Imp College Innovations Ltd Method
WO2003083125A1 (en) 2002-03-29 2003-10-09 Genencor International, Inc. Ehanced protein expression in bacillus
DK1511835T5 (en) 2002-06-07 2015-05-18 Dsm Ip Assets Bv IMPROVED PROCEDURE FOR PREVENTION OR REDUCTION OF BEVERAGES
TWI328612B (en) 2002-07-25 2010-08-11 Dsm Ip Assets Bv Genes and their encoded polypeptides involved in the biosynthetic pathway of vitamin b12, vectors and host cells comprising the genes, and process for producing vitamin b12
US20100129862A1 (en) 2003-05-12 2010-05-27 Jones Brian E Novel lipolytic Enzyme lip1
GB0403864D0 (en) 2004-02-20 2004-03-24 Ucl Ventures Modulator
CN101316928B (zh) 2005-11-28 2014-12-10 帝斯曼知识产权资产管理有限公司 产生清洁味道的酶制剂
GB0600406D0 (en) 2006-01-10 2006-02-15 Univ Bath Crystal
NZ578882A (en) 2007-02-20 2012-01-12 Dsm Ip Assets Bv Sialidase from the fungus Penicilium chrysogenum
KR20090129425A (ko) 2007-03-12 2009-12-16 다니스코 유에스 인크. 개질된 프로테아제
JP2010521188A (ja) 2007-03-22 2010-06-24 ディーエスエム アイピー アセッツ ビー.ブイ. 新規リシルオキシダーゼ
US8343735B2 (en) 2007-05-10 2013-01-01 Danisco Us Inc. Modified secretion system to increase expression of polypeptides in bacteria
GB0715751D0 (en) * 2007-08-13 2007-09-19 Tmo Renewables Ltd Thermophilic micro-organisms for ethanol production
US8512982B2 (en) 2007-12-21 2013-08-20 Danisco Us Inc. Enhanced protein production in Bacillus
NZ587540A (en) 2008-03-28 2012-06-29 Danisco Us Inc Method for amplifying locus in bacterial cell
CN102414321A (zh) 2009-04-24 2012-04-11 丹尼斯科美国公司 具有修饰的原区的蛋白酶
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
DK2510094T3 (en) 2009-12-09 2017-03-13 Danisco Us Inc COMPOSITIONS AND METHODS OF COMPREHENSIVE PROTEASE VARIETIES
WO2011101339A2 (en) 2010-02-18 2011-08-25 Dsm Ip Assets B.V. Fermentation broth and filtration filter cake and uses thereof
US20110281327A1 (en) 2010-04-15 2011-11-17 Danisco Us Inc. Compositions And Methods Comprising Variant Proteases
HUE045202T2 (hu) 2010-05-06 2019-12-30 Procter & Gamble Fogyasztási cikkek proteáz variánsokkal
WO2012007445A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Mutarotase in crystallization
WO2012007446A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Use of a mutarotase in the production of dried powders
TR201901382T4 (tr) 2011-05-05 2019-02-21 Danisco Inc Serin proteaz varyantlarını içeren bileşimler ve yöntemler.
MX357386B (es) 2011-05-05 2018-07-06 Procter & Gamble Composiciones y metodos que comprenden variantes de proteasa serina.
CN102296042B (zh) * 2011-08-26 2013-04-24 甘肃农业大学 地衣芽孢杆菌及用其制备凝乳酶冻干粉的方法
WO2013033318A1 (en) 2011-08-31 2013-03-07 Danisco Us Inc. Compositions and methods comprising a lipolytic enzyme variant
WO2013096653A1 (en) 2011-12-22 2013-06-27 Danisco Us Inc. Compositions and methods comprising a lipolytic enzyme variant
KR20150067336A (ko) 2012-10-12 2015-06-17 다니스코 유에스 인크. 지방분해 효소 변이체를 포함하는 조성물 및 방법
CN104781400A (zh) 2012-11-05 2015-07-15 丹尼斯科美国公司 包含嗜热菌蛋白酶变体的组合物和方法
EP2935573A1 (en) 2012-12-19 2015-10-28 Danisco US Inc. Novel mannanase, compositions and methods of use thereof
EP3636662B1 (en) 2013-05-29 2022-07-13 Danisco US Inc. Novel metalloproteases
US20160160202A1 (en) 2013-05-29 2016-06-09 Danisco Us Inc. Novel metalloproteases
EP3004342B1 (en) 2013-05-29 2023-01-11 Danisco US Inc. Novel metalloproteases
EP3004341B1 (en) 2013-05-29 2017-08-30 Danisco US Inc. Novel metalloproteases
EP3022299B1 (en) 2013-07-19 2020-03-18 Danisco US Inc. Compositions and methods comprising a lipolytic enzyme variant
EP3653707B1 (en) 2013-09-12 2025-07-16 Danisco US Inc. Compositions and methods comprising lg12-clade protease variants
EP3553173B1 (en) 2013-12-13 2021-05-19 Danisco US Inc. Serine proteases of the bacillus gibsonii-clade
DK3080262T3 (da) 2013-12-13 2019-05-06 Danisco Us Inc Serinproteaser af bacillus-arter
JP6585602B2 (ja) 2013-12-31 2019-10-02 ダニスコ・ユーエス・インク タンパク質発現の増大
DK3119884T3 (da) 2014-03-21 2019-10-14 Danisco Us Inc Serinproteaser af bacillus-arter
WO2016061438A1 (en) 2014-10-17 2016-04-21 Danisco Us Inc. Serine proteases of bacillus species
WO2016069557A1 (en) 2014-10-27 2016-05-06 Danisco Us Inc. Serine proteases of bacillus species
EP3957729A1 (en) 2014-10-27 2022-02-23 Danisco US Inc. Serine proteases
EP3212662B1 (en) 2014-10-27 2020-04-08 Danisco US Inc. Serine proteases
DK3212781T3 (da) 2014-10-27 2019-12-16 Danisco Us Inc Serinproteaser
EP4403631A3 (en) 2014-10-27 2024-10-30 Danisco US Inc. Serine proteases
EP3233893B1 (en) 2014-12-16 2021-01-20 Danisco US Inc. Enhanced protein expression
KR20170093247A (ko) 2014-12-19 2017-08-14 다니스코 유에스 인크. 향상된 단백질 발현
EP3611259A1 (en) 2015-03-12 2020-02-19 Danisco US Inc. Compositions and methods comprising lg12-clade protease variants
JP7274819B2 (ja) 2015-05-13 2023-05-17 ダニスコ・ユーエス・インク AprL-CLADEプロテアーゼ変異体及びその使用
CN107922934A (zh) 2015-06-17 2018-04-17 丹尼斯科美国公司 具有经修饰的前肽区域的蛋白酶
JP7015695B2 (ja) 2015-06-17 2022-02-03 ダニスコ・ユーエス・インク バチルス・ギブソニイ(Bacillus gibsonii)クレードセリンプロテアーゼ
US20190194636A1 (en) 2016-05-03 2019-06-27 Danisco Us Inc Protease variants and uses thereof
EP3452584B1 (en) 2016-05-05 2021-01-06 Danisco US Inc. Protease variants and uses thereof
MX2018014768A (es) 2016-05-31 2019-03-06 Danisco Us Inc Variantes de proteasa y sus usos.
JP7152319B2 (ja) 2016-06-17 2022-10-12 ダニスコ・ユーエス・インク プロテアーゼ変異体およびその使用
WO2018118950A1 (en) 2016-12-21 2018-06-28 Danisco Us Inc. Bacillus gibsonii-clade serine proteases
EP3559227B1 (en) 2016-12-21 2025-02-19 Danisco US Inc. Protease variants and uses thereof
WO2019108599A1 (en) 2017-11-29 2019-06-06 Danisco Us Inc Subtilisin variants having improved stability
WO2019245704A1 (en) 2018-06-19 2019-12-26 Danisco Us Inc Subtilisin variants
EP3799601A1 (en) 2018-06-19 2021-04-07 Danisco US Inc. Subtilisin variants
US20240279627A1 (en) 2018-08-30 2024-08-22 Danisco Us Inc Compositions comprising a lipolytic enzyme variant and methods of use thereof
EP3887515A1 (en) 2018-11-28 2021-10-06 Danisco US Inc. Subtilisin variants having improved stability
WO2020242858A1 (en) 2019-05-24 2020-12-03 Danisco Us Inc Subtilisin variants and methods of use
EP4090727A1 (en) 2020-01-13 2022-11-23 Danisco US Inc. Compositions comprising a lipolytic enzyme variant and methods of use thereof
US20240034960A1 (en) 2020-08-27 2024-02-01 Danisco Us Inc Enzymes and enzyme compositions for cleaning
WO2023278297A1 (en) 2021-06-30 2023-01-05 Danisco Us Inc Variant lipases and uses thereof
CN117916354A (zh) 2021-09-03 2024-04-19 丹尼斯科美国公司 用于清洁的衣物洗涤组合物
WO2023114794A1 (en) 2021-12-16 2023-06-22 The Procter & Gamble Company Fabric and home care composition comprising a protease
WO2023114936A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
CN118679252A (zh) 2021-12-16 2024-09-20 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
CA3240641A1 (en) 2021-12-16 2023-06-22 Michelle Jackson Automatic dishwashing composition comprising a protease
EP4448750A2 (en) 2021-12-16 2024-10-23 Danisco US Inc. Subtilisin variants and uses thereof
US20250163396A1 (en) 2022-03-01 2025-05-22 Danisco Us Inc. Enzymes and enzyme compositions for cleaning
WO2024050346A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc. Detergent compositions and methods related thereto
EP4581138A1 (en) 2022-09-02 2025-07-09 Danisco US Inc. Subtilisin variants and methods related thereto
WO2024102698A1 (en) 2022-11-09 2024-05-16 Danisco Us Inc. Subtilisin variants and methods of use
EP4658776A1 (en) 2023-02-01 2025-12-10 Danisco US Inc. Subtilisin variants and methods of use
US20240263162A1 (en) 2023-02-01 2024-08-08 The Procter & Gamble Company Detergent compositions containing enzymes
EP4677080A1 (en) 2023-03-06 2026-01-14 Danisco US Inc. Subtilisin variants and methods of use
WO2025071996A1 (en) 2023-09-28 2025-04-03 Danisco Us Inc. Variant cutinase enzymes with improved solubility and uses thereof
WO2025085351A1 (en) 2023-10-20 2025-04-24 Danisco Us Inc. Subtilisin variants and methods of use
WO2026024921A1 (en) 2024-07-25 2026-01-29 The Procter & Gamble Company Detergent composition comprising a subtilisin variant and methods of use

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO159863C (no) * 1980-01-07 1989-02-15 Univ Rochester Fremgangsm te for fremstilling og seleksjon av en rant bakteriofag som inneholder et genetisk fragment og koder for alfa-amylase, egnet for bruk i heterolog transformering av en bacillus-verts-mikroorganisme.
IL61982A (en) * 1980-02-15 1984-01-31 Cpc International Inc Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes
ZA81424B (en) * 1980-02-15 1982-02-24 Cpc International Inc Genetically engineered microorganisms for massive production of amylolytic enzymes and process for preparing same
JPS56137896A (en) * 1980-03-10 1981-10-28 Cetus Corp Genetic recombination gram positive bacteria , production thereof and protein for mammal produced by said gram positive bacteria
AU545912B2 (en) * 1980-03-10 1985-08-08 Cetus Corporation Cloned heterologous jive products in bacillies
FI64813C (fi) * 1980-12-31 1984-01-10 Ilkka Antero Palva Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer
CA1206108A (en) * 1981-03-06 1986-06-17 Shuichi Aiba Process for transforming microorganism by means of plasmid introduction
BR8205954A (pt) * 1981-10-14 1983-09-13 Unilever Nv Sequencia de dna,plasmidio recombinante,cultura bacteriana e microorganismos
JPS5889194A (ja) * 1981-11-24 1983-05-27 Ajinomoto Co Inc 微生物によるl−トリプトフアンの製造法
JPS58107192A (ja) * 1981-12-18 1983-06-25 Ajinomoto Co Inc 発酵法によるl−ヒスチジンの製造法

Also Published As

Publication number Publication date
DK101385D0 (da) 1985-03-05
WO1985000382A1 (en) 1985-01-31
DK101385A (da) 1985-03-05
NZ208806A (en) 1988-07-28
JP2669457B2 (ja) 1997-10-27
ES8609467A1 (es) 1986-08-01
AU570709B2 (en) 1988-03-24
ES545256A0 (es) 1986-08-01
AU3151084A (en) 1985-02-07
IE58014B1 (en) 1993-06-16
FI85287C (fi) 1992-03-25
EP0134048B1 (en) 1989-11-08
ES534102A0 (es) 1986-06-16
DK169996B1 (da) 1995-04-24
IE841726L (en) 1985-01-06
DE3480411D1 (en) 1989-12-14
JPS60501738A (ja) 1985-10-17
JP2664860B2 (ja) 1997-10-22
FI850845L (fi) 1985-03-01
EP0134048B2 (en) 1996-08-14
FI85287B (fi) 1991-12-13
GR82200B (esLanguage) 1984-12-13
JPH06189783A (ja) 1994-07-12
FI850845A0 (fi) 1985-03-01
EP0134048A1 (en) 1985-03-13

Similar Documents

Publication Publication Date Title
ES8609467A1 (es) Procedimiento para preparar un polipetido de interes en una cepa de bacilus industrial
GB8405129D0 (en) Yeast promoter
ES8404409A1 (es) Un metodo de expresion de un gen.
DE3471688D1 (en) Dna vectors and their use in recombinant dna technology
ES8607388A1 (es) Un metodo para preparar que tiene sustancialmente la misma secuencia de aminoacidos que la superoxido-dismutasa humana.
Woods et al. Differential roles of the Escherichia coli fumarases and fnr-dependent expression of fumarase B and aspartase
AU551251B2 (en) Recombinant dna; chymosin production
EP1170362A3 (en) Cloning and expressing restriction endonucleases and modification methylases from caryophanon
AU551490B2 (en) Dna code for protein to suppress abnormal protein degradation
KR940002352A (ko) 디(d)-아미노산 옥시다제 생산성 형질전환체
Bonamy et al. Cloning and expression of Bacillus subtilis spore genes
DE3380878D1 (en) Method of heterologous cloning of gene in bacillus microorganism
EP0165002A3 (en) Recombinant dna, microorganisms containing same and their use in the production of amylases
DESROCHERS Molecular cloning of a β-xylosidase gene from Bacillus subtilis
Coppolecchia et al. Cloning in E. coli of a Streptomyces cellulase gene
WO1986000929A1 (en) Recombinant dna molecule, transformed microorganisms and process for producing penicillin v amidase
JPS6467181A (en) Microorganism containing plasmid produced by gene recombination and production of lichenase with said microorganism
ATE122097T1 (de) Expressionsvektor und verfahren zur regulierbaren herstellung von proteinen in eukaryonten.
TW373022B (en) Molecular cloning of amylase gene and antibiotics production in streptomyces
JPS5978691A (ja) 高度好熱菌に由来する新規なプラスミド
JPS5978683A (ja) 新規なプラスミドを保有する新規な微生物
JPS6437284A (en) Bacillus subtilis having lowered protease productivity
JPS6416586A (en) Production of baniii restriction enzyme
JPS6416585A (en) Production of bani restriction enzyme

Legal Events

Date Code Title Description
PC1A Transfer granted
FD1A Patent lapsed

Effective date: 20060405