JPS6416585A - Production of bani restriction enzyme - Google Patents

Production of bani restriction enzyme

Info

Publication number
JPS6416585A
JPS6416585A JP62171746A JP17174687A JPS6416585A JP S6416585 A JPS6416585 A JP S6416585A JP 62171746 A JP62171746 A JP 62171746A JP 17174687 A JP17174687 A JP 17174687A JP S6416585 A JPS6416585 A JP S6416585A
Authority
JP
Japan
Prior art keywords
restriction endonuclease
plasmid
bani
bani restriction
pban11
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP62171746A
Other languages
Japanese (ja)
Other versions
JPH0630576B2 (en
Inventor
Fumikiyo Kawakami
Yoshihiko Maekawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toyobo Co Ltd
Original Assignee
Toyobo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toyobo Co Ltd filed Critical Toyobo Co Ltd
Priority to JP62171746A priority Critical patent/JPH0630576B2/en
Publication of JPS6416585A publication Critical patent/JPS6416585A/en
Publication of JPH0630576B2 publication Critical patent/JPH0630576B2/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE:To easily produce the titled substance in large quantity, by inserting a structural gene of a BanI restriction endonuclease originate from Bacillus aneurinolyticus into a vector plasmid pBR322 and introducing the plasmid into E.coli. CONSTITUTION:A chromosome DNA containing BanI restriction endonuclease gene originated from Bacillus aneurinolyticus IAM1077 is prepared and introduced into BamHI site of pBR322 to obtain a plasmid pBAN11 of 8kb. The plasmid is introduced into Escherichia coli HB101 strain to obtain a transformed Escherichia coli HB101 (pBAN11) (FERM P-9424). The strain is cultured in a nutrient medium to obtain BanI restriction endonuclease without necessitating the removal of BanIII and BanII restriction endonuclease.
JP62171746A 1987-07-09 1987-07-09 Ban I Method for producing restricted endonuclease Expired - Fee Related JPH0630576B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62171746A JPH0630576B2 (en) 1987-07-09 1987-07-09 Ban I Method for producing restricted endonuclease

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62171746A JPH0630576B2 (en) 1987-07-09 1987-07-09 Ban I Method for producing restricted endonuclease

Publications (2)

Publication Number Publication Date
JPS6416585A true JPS6416585A (en) 1989-01-20
JPH0630576B2 JPH0630576B2 (en) 1994-04-27

Family

ID=15928922

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62171746A Expired - Fee Related JPH0630576B2 (en) 1987-07-09 1987-07-09 Ban I Method for producing restricted endonuclease

Country Status (1)

Country Link
JP (1) JPH0630576B2 (en)

Also Published As

Publication number Publication date
JPH0630576B2 (en) 1994-04-27

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Legal Events

Date Code Title Description
LAPS Cancellation because of no payment of annual fees