JPS6467179A - L-tryptophane-producing bacterium and production of l-tryptophane - Google Patents

L-tryptophane-producing bacterium and production of l-tryptophane

Info

Publication number
JPS6467179A
JPS6467179A JP22400587A JP22400587A JPS6467179A JP S6467179 A JPS6467179 A JP S6467179A JP 22400587 A JP22400587 A JP 22400587A JP 22400587 A JP22400587 A JP 22400587A JP S6467179 A JPS6467179 A JP S6467179A
Authority
JP
Japan
Prior art keywords
plasmid
bacillus
tryptophane
bacterium
genus bacillus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP22400587A
Other languages
Japanese (ja)
Inventor
Kazunori Sakimoto
Nariaki Tarusawa
Takashi Kondo
Keiko Miyao
Akio Ichinori
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Resonac Holdings Corp
Original Assignee
Showa Denko KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Showa Denko KK filed Critical Showa Denko KK
Priority to JP22400587A priority Critical patent/JPS6467179A/en
Publication of JPS6467179A publication Critical patent/JPS6467179A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PURPOSE:To readily produce the titled substance in a large quantity, by inserting a gene coding PDG prepared by a bacterium of genus Bacillus into a plasmid vector capable of multiplying in a bacterium of genus Bacillus and subsequently introducing the inserted vector into a bacterium of genus Bacillus. CONSTITUTION:A plasmid pSDR 0023 containing a gene coding PGD (3- phosphoglycerate dehydrogenase) is prepared from a gene DNA of bacillus amyloliquefaciens SD-31 (FERM No.6840). A 0.9 mega dalton ECoRI fragment of the plasmid is recloned in a Bacillus subtilis pLS 15 to give a plasmid pSDM 00350. The plasmid is introduced into a genus Bacillus host to give a transformed strain Bacillus amyloliquefaciens SD-1021 or Bacillus subtilis SD-1022. These strains are cultured in a medium containing anthranilic acid, indole or a salt thereof to provide L-tryptophane.
JP22400587A 1987-09-09 1987-09-09 L-tryptophane-producing bacterium and production of l-tryptophane Pending JPS6467179A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP22400587A JPS6467179A (en) 1987-09-09 1987-09-09 L-tryptophane-producing bacterium and production of l-tryptophane

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP22400587A JPS6467179A (en) 1987-09-09 1987-09-09 L-tryptophane-producing bacterium and production of l-tryptophane

Publications (1)

Publication Number Publication Date
JPS6467179A true JPS6467179A (en) 1989-03-13

Family

ID=16807098

Family Applications (1)

Application Number Title Priority Date Filing Date
JP22400587A Pending JPS6467179A (en) 1987-09-09 1987-09-09 L-tryptophane-producing bacterium and production of l-tryptophane

Country Status (1)

Country Link
JP (1) JPS6467179A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0401735A1 (en) 1989-06-06 1990-12-12 Kyowa Hakko Kogyo Co., Ltd. Process for producing -L-tryptophan

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0401735A1 (en) 1989-06-06 1990-12-12 Kyowa Hakko Kogyo Co., Ltd. Process for producing -L-tryptophan
US5407824A (en) * 1989-06-06 1995-04-18 Kyowa Hakko Kogyo Co., Ltd. Recombinant coryneform bacterium for producing L-tryptophan
US5447857A (en) * 1989-06-06 1995-09-05 Kyowa Hakko Kogyo Co., Ltd. Process for producing L-tryptophan

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