ES2745694T3 - Métodos y composiciones para normalización de biblioteca de ácido nucleico - Google Patents
Métodos y composiciones para normalización de biblioteca de ácido nucleico Download PDFInfo
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- ES2745694T3 ES2745694T3 ES16770134T ES16770134T ES2745694T3 ES 2745694 T3 ES2745694 T3 ES 2745694T3 ES 16770134 T ES16770134 T ES 16770134T ES 16770134 T ES16770134 T ES 16770134T ES 2745694 T3 ES2745694 T3 ES 2745694T3
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| CA2865575C (en) | 2012-02-27 | 2024-01-16 | Cellular Research, Inc. | Compositions and kits for molecular counting |
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| WO2016196229A1 (en) | 2015-06-01 | 2016-12-08 | Cellular Research, Inc. | Methods for rna quantification |
| JP6940484B2 (ja) | 2015-09-11 | 2021-09-29 | セルラー リサーチ， インコーポレイテッド | ライブラリー正規化のための方法および組成物 |
| JP7129343B2 (ja) | 2016-05-02 | 2022-09-01 | ベクトン・ディキンソン・アンド・カンパニー | 正確な分子バーコーディング |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
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| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| CN109791157B (zh) | 2016-09-26 | 2022-06-07 | 贝克顿迪金森公司 | 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达 |
| JP7228510B2 (ja) | 2016-11-08 | 2023-02-24 | ベクトン・ディキンソン・アンド・カンパニー | 細胞標識分類の方法 |
| US11164659B2 (en) | 2016-11-08 | 2021-11-02 | Becton, Dickinson And Company | Methods for expression profile classification |
| WO2018119399A1 (en) | 2016-12-23 | 2018-06-28 | Grail, Inc. | Methods for high efficiency library preparation using double-stranded adapters |
| ES2961580T3 (es) | 2017-01-13 | 2024-03-12 | Cellular Res Inc | Revestimiento hidrófilo de canales de fluidos |
| CN110382708A (zh) | 2017-02-01 | 2019-10-25 | 赛卢拉研究公司 | 使用阻断性寡核苷酸进行选择性扩增 |
| US11274344B2 (en) | 2017-03-30 | 2022-03-15 | Grail, Inc. | Enhanced ligation in sequencing library preparation |
| WO2018183897A1 (en) | 2017-03-31 | 2018-10-04 | Grail, Inc. | Higher target capture efficiency using probe extension |
| CN110719959B (zh) | 2017-06-05 | 2021-08-06 | 贝克顿迪金森公司 | 针对单细胞的样品索引 |
| US20200123539A1 (en) * | 2017-06-20 | 2020-04-23 | uBiome, Inc. | Method and system for library preparation with unique molecular identifiers |
| WO2019051319A1 (en) * | 2017-09-08 | 2019-03-14 | uBiome, Inc. | STANDARDIZATION FOR SEQUENCING BANKS |
| WO2019099574A1 (en) | 2017-11-15 | 2019-05-23 | Viome, Inc. | Methods and compositions for preparing polynucleotide libraries |
| WO2019113563A1 (en) * | 2017-12-09 | 2019-06-13 | Viome, Inc. | Methods for nucleic acid library creation |
| EP3728636B1 (en) | 2017-12-19 | 2024-09-11 | Becton, Dickinson and Company | Particles associated with oligonucleotides |
| JP7358388B2 (ja) | 2018-05-03 | 2023-10-10 | ベクトン・ディキンソン・アンド・カンパニー | 反対側の転写物末端における分子バーコーディング |
| CN112272710A (zh) | 2018-05-03 | 2021-01-26 | 贝克顿迪金森公司 | 高通量多组学样品分析 |
| ES2992135T3 (es) | 2018-10-01 | 2024-12-09 | Becton Dickinson Co | Determinar secuencias de transcripción 5 |
| US11932849B2 (en) | 2018-11-08 | 2024-03-19 | Becton, Dickinson And Company | Whole transcriptome analysis of single cells using random priming |
| US11492660B2 (en) | 2018-12-13 | 2022-11-08 | Becton, Dickinson And Company | Selective extension in single cell whole transcriptome analysis |
| JP2022515466A (ja) * | 2018-12-28 | 2022-02-18 | ナショナル ユニヴァーシティー オブ シンガポール | 標的化された相補的ｄｎａ富化のための方法 |
| US11371076B2 (en) | 2019-01-16 | 2022-06-28 | Becton, Dickinson And Company | Polymerase chain reaction normalization through primer titration |
| ES2945227T3 (es) | 2019-01-23 | 2023-06-29 | Becton Dickinson Co | Oligonucleótidos asociados con anticuerpos |
| WO2020167920A1 (en) | 2019-02-14 | 2020-08-20 | Cellular Research, Inc. | Hybrid targeted and whole transcriptome amplification |
| US11965208B2 (en) | 2019-04-19 | 2024-04-23 | Becton, Dickinson And Company | Methods of associating phenotypical data and single cell sequencing data |
| CN120099137A (zh) | 2019-07-22 | 2025-06-06 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
| JP7522189B2 (ja) | 2019-11-08 | 2024-07-24 | ベクトン・ディキンソン・アンド・カンパニー | 免疫レパートリーシーケンシングのための完全長ｖ（ｄ）ｊ情報を得るためのランダムプライミングの使用 |
| CN115038795B (zh) * | 2019-12-04 | 2026-03-20 | 贝克顿·迪金森公司 | 捕获磁珠介导的单颗粒中核酸靶标的分子条形码编码及用于其的组合物 |
| US11649497B2 (en) | 2020-01-13 | 2023-05-16 | Becton, Dickinson And Company | Methods and compositions for quantitation of proteins and RNA |
| WO2021146219A1 (en) | 2020-01-13 | 2021-07-22 | Becton, Dickinson And Company | Cell capture using du-containing oligonucleotides |
| WO2021155057A1 (en) | 2020-01-29 | 2021-08-05 | Becton, Dickinson And Company | Barcoded wells for spatial mapping of single cells through sequencing |
| CN115427584A (zh) * | 2020-01-31 | 2022-12-02 | 贝克顿迪金森公司 | 嗜中温dna聚合酶延伸阻断物 |
| WO2021173719A1 (en) | 2020-02-25 | 2021-09-02 | Becton, Dickinson And Company | Bi-specific probes to enable the use of single-cell samples as single color compensation control |
| US11661625B2 (en) | 2020-05-14 | 2023-05-30 | Becton, Dickinson And Company | Primers for immune repertoire profiling |
| US12157913B2 (en) | 2020-06-02 | 2024-12-03 | Becton, Dickinson And Company | Oligonucleotides and beads for 5 prime gene expression assay |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| US12391940B2 (en) | 2020-07-31 | 2025-08-19 | Becton, Dickinson And Company | Single cell assay for transposase-accessible chromatin |
| EP4247967A1 (en) | 2020-11-20 | 2023-09-27 | Becton, Dickinson and Company | Profiling of highly expressed and lowly expressed proteins |
| CN116685850A (zh) | 2020-12-15 | 2023-09-01 | 贝克顿迪金森公司 | 单细胞分泌组分析 |
| CN112680797B (zh) * | 2021-02-04 | 2023-09-26 | 广州大学 | 一种去除高丰度rna的测序文库及其构建方法 |
| WO2022248237A1 (en) * | 2021-05-24 | 2022-12-01 | F. Hoffmann-La Roche Ag | Enhancer oligonucleotides for nucleic acid hybridization |
| CN118434839A (zh) * | 2021-12-24 | 2024-08-02 | 深圳华大生命科学研究院 | 提升芯片探针数量的dna文库序列及其应用 |
| US20230399679A1 (en) * | 2022-06-09 | 2023-12-14 | GTSeek, LLC | System and method for ligand-limited normalizing polymerase chain reaction (lln-pcr) |
| GB2621159B (en) * | 2022-08-04 | 2025-08-13 | Wobble Genomics Ltd | Methods of preparing processed nucleic acid samples and detecting nucleic acids and devices therefor |
| AU2023364547A1 (en) | 2022-10-21 | 2025-04-10 | Watchmaker Genomics, Inc. | Methods and compositions for sequencing library normalization |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4510244A (en) | 1980-04-17 | 1985-04-09 | The Board Of Trustees Of The Leland Stanford Jr. University | Cell labeling with antigen-coupled microspheres |
| US4725536A (en) | 1985-09-19 | 1988-02-16 | Genetics Institute, Inc. | Reagent polynucleotide complex with multiple target binding regions, and kit and methods |
| US6150517A (en) | 1986-11-24 | 2000-11-21 | Gen-Probe | Methods for making oligonucleotide probes for the detection and/or quantitation of non-viral organisms |
| WO1989001050A1 (en) | 1987-07-31 | 1989-02-09 | The Board Of Trustees Of The Leland Stanford Junior University | Selective amplification of target polynucleotide sequences |
| US5149625A (en) | 1987-08-11 | 1992-09-22 | President And Fellows Of Harvard College | Multiplex analysis of DNA |
| US5124246A (en) | 1987-10-15 | 1992-06-23 | Chiron Corporation | Nucleic acid multimers and amplified nucleic acid hybridization assays using same |
| US5656731A (en) | 1987-10-15 | 1997-08-12 | Chiron Corporation | Nucleic acid-amplified immunoassay probes |
| EP0426756A4 (en) | 1988-07-26 | 1991-11-21 | Genelabs Incorporated | Rna and dna amplification techniques |
| US6551784B2 (en) | 1989-06-07 | 2003-04-22 | Affymetrix Inc | Method of comparing nucleic acid sequences |
| US5800992A (en) | 1989-06-07 | 1998-09-01 | Fodor; Stephen P.A. | Method of detecting nucleic acids |
| US5925525A (en) | 1989-06-07 | 1999-07-20 | Affymetrix, Inc. | Method of identifying nucleotide differences |
| US5871928A (en) | 1989-06-07 | 1999-02-16 | Fodor; Stephen P. A. | Methods for nucleic acid analysis |
| US6309822B1 (en) | 1989-06-07 | 2001-10-30 | Affymetrix, Inc. | Method for comparing copy number of nucleic acid sequences |
| US5744101A (en) | 1989-06-07 | 1998-04-28 | Affymax Technologies N.V. | Photolabile nucleoside protecting groups |
| US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| US6040138A (en) | 1995-09-15 | 2000-03-21 | Affymetrix, Inc. | Expression monitoring by hybridization to high density oligonucleotide arrays |
| US5424186A (en) | 1989-06-07 | 1995-06-13 | Affymax Technologies N.V. | Very large scale immobilized polymer synthesis |
| US5200314A (en) | 1990-03-23 | 1993-04-06 | Chiron Corporation | Polynucleotide capture assay employing in vitro amplification |
| JP3164814B2 (ja) * | 1990-08-27 | 2001-05-14 | 科学技術振興事業団 | 均一化ｃＤＮＡライブラリーの作製法 |
| EP0834575B1 (en) | 1990-12-06 | 2001-11-28 | Affymetrix, Inc. (a Delaware Corporation) | Identification of nucleic acids in samples |
| JPH04337446A (ja) | 1991-05-15 | 1992-11-25 | Hitachi Ltd | 微粒子計測方法、定量方法および微粒子計測装置 |
| US5981179A (en) | 1991-11-14 | 1999-11-09 | Digene Diagnostics, Inc. | Continuous amplification reaction |
| US5424413A (en) | 1992-01-22 | 1995-06-13 | Gen-Probe Incorporated | Branched nucleic acid probes |
| US5573905A (en) | 1992-03-30 | 1996-11-12 | The Scripps Research Institute | Encoded combinatorial chemical libraries |
| US5981176A (en) | 1992-06-17 | 1999-11-09 | City Of Hope | Method of detecting and discriminating between nucleic acid sequences |
| DE69431719T2 (de) | 1993-06-25 | 2003-09-18 | Affymetrix, Inc. (N.D.Ges.D.Staates Delaware) | Hybridisierung und sequenzierung von nukleinsäuren |
| US6087186A (en) | 1993-07-16 | 2000-07-11 | Irori | Methods and apparatus for synthesizing labeled combinatorial chemistry libraries |
| US5500356A (en) | 1993-08-10 | 1996-03-19 | Life Technologies, Inc. | Method of nucleic acid sequence selection |
| US6309823B1 (en) | 1993-10-26 | 2001-10-30 | Affymetrix, Inc. | Arrays of nucleic acid probes for analyzing biotransformation genes and methods of using the same |
| US5681697A (en) | 1993-12-08 | 1997-10-28 | Chiron Corporation | Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor |
| US5714330A (en) | 1994-04-04 | 1998-02-03 | Lynx Therapeutics, Inc. | DNA sequencing by stepwise ligation and cleavage |
| US6495518B1 (en) | 1994-06-13 | 2002-12-17 | Vanderbilt University | Method for importing biologically active molecules into cells |
| GB2293238A (en) | 1994-09-13 | 1996-03-20 | Inceltec Ltd | Primers for replication and/or amplification reactions |
| US6013445A (en) | 1996-06-06 | 2000-01-11 | Lynx Therapeutics, Inc. | Massively parallel signature sequencing by ligation of encoded adaptors |
| US5846719A (en) | 1994-10-13 | 1998-12-08 | Lynx Therapeutics, Inc. | Oligonucleotide tags for sorting and identification |
| US5604097A (en) | 1994-10-13 | 1997-02-18 | Spectragen, Inc. | Methods for sorting polynucleotides using oligonucleotide tags |
| US5695934A (en) | 1994-10-13 | 1997-12-09 | Lynx Therapeutics, Inc. | Massively parallel sequencing of sorted polynucleotides |
| US6600996B2 (en) | 1994-10-21 | 2003-07-29 | Affymetrix, Inc. | Computer-aided techniques for analyzing biological sequences |
| DE69535240T2 (de) | 1994-10-28 | 2007-06-06 | Gen-Probe Inc., San Diego | Zusammensetzungen und Verfahren für die gleichzeitige Detektion und Quantifizierung von einer Mehrheit spezifischer Nuklein Säure Sequenzen |
| CA2216645A1 (en) | 1995-04-25 | 1996-11-21 | Irori | Remotely programmable matrices with memories and uses thereof |
| US5648245A (en) | 1995-05-09 | 1997-07-15 | Carnegie Institution Of Washington | Method for constructing an oligonucleotide concatamer library by rolling circle replication |
| US5968740A (en) | 1995-07-24 | 1999-10-19 | Affymetrix, Inc. | Method of Identifying a Base in a Nucleic Acid |
| US5763175A (en) | 1995-11-17 | 1998-06-09 | Lynx Therapeutics, Inc. | Simultaneous sequencing of tagged polynucleotides |
| US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| US6613544B1 (en) | 1995-12-22 | 2003-09-02 | Amgen Inc. | Osteoprotegerin |
| US5962271A (en) | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
| US5830712A (en) | 1996-02-06 | 1998-11-03 | Allelix Biopharmaceuticals Inc. | Selective template deletion method |
| US6458530B1 (en) | 1996-04-04 | 2002-10-01 | Affymetrix Inc. | Selecting tag nucleic acids |
| EP2369007B1 (en) | 1996-05-29 | 2015-07-29 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions |
| JPH1021373A (ja) | 1996-07-05 | 1998-01-23 | Fuji Photo Film Co Ltd | 画像解析装置 |
| US20020172953A1 (en) | 1996-07-29 | 2002-11-21 | Mirkin Chad A. | Movement of biomolecule-coated nanoparticles in an electric field |
| US7169556B2 (en) | 1996-07-29 | 2007-01-30 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
| US6984491B2 (en) | 1996-07-29 | 2006-01-10 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
| US6100029A (en) | 1996-08-14 | 2000-08-08 | Exact Laboratories, Inc. | Methods for the detection of chromosomal aberrations |
| US5935793A (en) | 1996-09-27 | 1999-08-10 | The Chinese University Of Hong Kong | Parallel polynucleotide sequencing method using tagged primers |
| US6124092A (en) | 1996-10-04 | 2000-09-26 | The Perkin-Elmer Corporation | Multiplex polynucleotide capture methods and compositions |
| US6117631A (en) | 1996-10-29 | 2000-09-12 | Polyprobe, Inc. | Detection of antigens via oligonucleotide antibody conjugates |
| US6046005A (en) | 1997-01-15 | 2000-04-04 | Incyte Pharmaceuticals, Inc. | Nucleic acid sequencing with solid phase capturable terminators comprising a cleavable linking group |
| JP4294740B2 (ja) | 1997-05-23 | 2009-07-15 | ソレクサ・インコーポレイテッド | 分析物の系列的プロセシングのためのシステムおよび装置 |
| US6974669B2 (en) | 2000-03-28 | 2005-12-13 | Nanosphere, Inc. | Bio-barcodes based on oligonucleotide-modified nanoparticles |
| US6399334B1 (en) * | 1997-09-24 | 2002-06-04 | Invitrogen Corporation | Normalized nucleic acid libraries and methods of production thereof |
| AU1603199A (en) | 1997-12-03 | 1999-06-16 | Curagen Corporation | Methods and devices for measuring differential gene expression |
| US6265163B1 (en) | 1998-01-09 | 2001-07-24 | Lynx Therapeutics, Inc. | Solid phase selection of differentially expressed genes |
| US6787308B2 (en) | 1998-07-30 | 2004-09-07 | Solexa Ltd. | Arrayed biomolecules and their use in sequencing |
| WO2000014282A1 (en) | 1998-09-04 | 2000-03-16 | Lynx Therapeutics, Inc. | Method of screening for genetic polymorphism |
| US6480791B1 (en) | 1998-10-28 | 2002-11-12 | Michael P. Strathmann | Parallel methods for genomic analysis |
| US6197554B1 (en) * | 1998-11-20 | 2001-03-06 | Shi-Lung Lin | Method for generating full-length cDNA library from single cells |
| US6436675B1 (en) | 1999-09-28 | 2002-08-20 | Maxygen, Inc. | Use of codon-varied oligonucleotide synthesis for synthetic shuffling |
| US20020019005A1 (en) * | 1999-02-18 | 2002-02-14 | Arcaris, Inc. | Process for identification of genes encoding proteins having cell proliferation-promoting activity |
| US6629040B1 (en) | 1999-03-19 | 2003-09-30 | University Of Washington | Isotope distribution encoded tags for protein identification |
| JP2002539849A (ja) | 1999-03-26 | 2002-11-26 | ホワイトヘッド インスチチュート フォアー バイオメディカル リサーチ | ユニバーサルアレイ |
| EP1046717B1 (en) | 1999-04-20 | 2010-10-06 | National Institute of Advanced Industrial Science and Technology | Method and probes for determining a concentration of target nucleic acid molecules and method for analyzing data obtained by the method |
| US6355431B1 (en) | 1999-04-20 | 2002-03-12 | Illumina, Inc. | Detection of nucleic acid amplification reactions using bead arrays |
| US6372813B1 (en) | 1999-06-25 | 2002-04-16 | Motorola | Methods and compositions for attachment of biomolecules to solid supports, hydrogels, and hydrogel arrays |
| US6326148B1 (en) | 1999-07-12 | 2001-12-04 | The Regents Of The University Of California | Detection of copy number changes in colon cancer |
| US6440706B1 (en) | 1999-08-02 | 2002-08-27 | Johns Hopkins University | Digital amplification |
| JP2001078768A (ja) | 1999-09-16 | 2001-03-27 | Otsuka Pharmaceut Co Ltd | 遺伝子発現の測定法 |
| SE9903988D0 (sv) | 1999-11-03 | 1999-11-03 | Amersham Pharm Biotech Ab | Method of analysis |
| US6489114B2 (en) | 1999-12-17 | 2002-12-03 | Bio Merieux | Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby |
| WO2001048242A2 (en) | 1999-12-29 | 2001-07-05 | Mergen Ltd. | Methods for amplifying and detecting multiple polynucleotides on a solid phase support |
| DE60118527D1 (de) | 2000-01-24 | 2006-05-18 | Compound Therapeutics Inc | Sensible und multiplexe diagnostische tests zur proteinanalyse |
| US7022479B2 (en) | 2000-01-24 | 2006-04-04 | Compound Therapeutics, Inc. | Sensitive, multiplexed diagnostic assays for protein analysis |
| US6235483B1 (en) | 2000-01-31 | 2001-05-22 | Agilent Technologies, Inc. | Methods and kits for indirect labeling of nucleic acids |
| DK1259643T3 (da) | 2000-02-07 | 2009-02-23 | Illumina Inc | Fremgangsmåde til detektion af nukleinsyre ved anvendelse af universel priming |
| US20050214825A1 (en) | 2000-02-07 | 2005-09-29 | John Stuelpnagel | Multiplex sample analysis on universal arrays |
| GB2364054B (en) | 2000-03-24 | 2002-05-29 | Smithkline Beecham Corp | Method of amplifying quinolone-resistance-determining-regions and identifying polymorphic variants thereof |
| EP1278889B1 (en) * | 2000-03-29 | 2006-07-26 | LGC Limited | Hybridisation beacon and method of rapid sequence detection and discrimination |
| EP2278030B1 (en) | 2000-04-10 | 2017-05-24 | Taxon Biosciences, Inc. | Methods for the survey and genetic analysis of populations |
| US20030207300A1 (en) | 2000-04-28 | 2003-11-06 | Matray Tracy J. | Multiplex analytical platform using molecular tags |
| US7630063B2 (en) | 2000-08-02 | 2009-12-08 | Honeywell International Inc. | Miniaturized cytometer for detecting multiple species in a sample |
| US6511809B2 (en) | 2000-06-13 | 2003-01-28 | E. I. Du Pont De Nemours And Company | Method for the detection of an analyte by means of a nucleic acid reporter |
| ATE377093T1 (de) | 2000-07-07 | 2007-11-15 | Visigen Biotechnologies Inc | Sequenzbestimmung in echtzeit |
| AU2001283159A1 (en) * | 2000-08-07 | 2002-02-18 | Genemed Biotechnologies, Inc. | Methods for identifying low-abundance polynucleotides and related compositions |
| CA2418502A1 (en) | 2000-08-11 | 2002-03-07 | Nanosphere Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
| CA2356123A1 (en) | 2000-08-25 | 2002-02-25 | Riken | Method of preparing normalized and/or subtracted cdna |
| JP4915492B2 (ja) * | 2000-08-25 | 2012-04-11 | 独立行政法人理化学研究所 | ノーマライズ／サブトラクトされたｃＤＮＡライブラリーの作成方法 |
| US6934408B2 (en) | 2000-08-25 | 2005-08-23 | Amnis Corporation | Method and apparatus for reading reporter labeled beads |
| DE60131903T2 (de) | 2000-10-24 | 2008-11-27 | The Board of Trustees of the Leland S. Stanford Junior University, Palo Alto | Direkte multiplex charakterisierung von genomischer dna |
| WO2002046472A2 (en) | 2000-12-08 | 2002-06-13 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
| US20020142345A1 (en) | 2000-12-22 | 2002-10-03 | Nelsen Anita J. | Methods for encoding and decoding complex mixtures in arrayed assays |
| US20030049616A1 (en) | 2001-01-08 | 2003-03-13 | Sydney Brenner | Enzymatic synthesis of oligonucleotide tags |
| WO2002070684A2 (en) * | 2001-01-11 | 2002-09-12 | Lion Bioscience Ag | Gene library for screening methods |
| ES2382542T3 (es) | 2001-01-25 | 2012-06-11 | Luminex Molecular Diagnostics, Inc. | Polinucleótidos para su utilización como etiquetas y complementos de etiqueta, fabricación y utilización de los mismos |
| MXPA02012739A (es) | 2001-03-09 | 2004-04-20 | Nugen Technologies Inc | Metodos y composiciones para amplificacion de secuencias de arn. |
| JP2005233974A (ja) | 2001-03-21 | 2005-09-02 | Olympus Corp | 生化学的検査方法 |
| US7027932B2 (en) | 2001-03-21 | 2006-04-11 | Olympus Optical Co., Ltd. | Biochemical examination method |
| EP1379693B1 (en) | 2001-03-28 | 2009-05-20 | Nanosphere, Inc. | Bio-barcodes based on oligonucleotide-modified particles |
| US20030170675A1 (en) | 2001-04-11 | 2003-09-11 | The Gov't Of The U.S Of America As Represented By The Secretary Of The Dept. Of Health & Human Serv. | Methods of manipulating nucleic acids |
| US20050221304A1 (en) | 2001-04-11 | 2005-10-06 | Charlie Xiang | Modified random primers for probe labeling |
| AUPR480901A0 (en) | 2001-05-04 | 2001-05-31 | Genomics Research Partners Pty Ltd | Diagnostic method for assessing a condition of a performance animal |
| US6849404B2 (en) | 2001-05-07 | 2005-02-01 | Bioneer Corporation | Polymerase chain reaction of DNA of which base sequence is completely unidentified |
| US6905827B2 (en) | 2001-06-08 | 2005-06-14 | Expression Diagnostics, Inc. | Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases |
| EP1395805A4 (en) | 2001-06-11 | 2005-03-09 | Illumina Inc | MULTIPLEXED DETECTION TECHNIQUES |
| JP4244534B2 (ja) | 2001-06-12 | 2009-03-25 | 横河電機株式会社 | バイオチップ |
| US7473767B2 (en) | 2001-07-03 | 2009-01-06 | The Institute For Systems Biology | Methods for detection and quantification of analytes in complex mixtures |
| US6830931B2 (en) | 2001-07-12 | 2004-12-14 | Automated Cell, Inc. | Method and apparatus for monitoring of proteins and cells |
| AU2002363062B2 (en) | 2001-10-09 | 2007-03-22 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
| US20030077611A1 (en) | 2001-10-24 | 2003-04-24 | Sention | Methods and systems for dynamic gene expression profiling |
| US20030165935A1 (en) | 2001-11-21 | 2003-09-04 | Vann Charles S. | Digital assay |
| US20030175749A1 (en) | 2001-12-08 | 2003-09-18 | Jong-Yoon Chun | Annealing control primer and its uses |
| CN1617938A (zh) | 2002-01-16 | 2005-05-18 | 戴诺生物技术有限公司 | 从单个样品中分离核酸和蛋白质的方法 |
| JP2005515792A (ja) | 2002-01-29 | 2005-06-02 | グローバル ジェノミクス アクティエボラーグ | 核酸を操作するための方法および手段 |
| CA2474509C (en) | 2002-02-14 | 2012-01-31 | Immunivest Corporation | Methods and algorithms for cell enumeration in a low-cost cytometer |
| US20030186251A1 (en) | 2002-04-01 | 2003-10-02 | Brookhaven Science Associates, Llc | Genome sequence tags |
| US20050175993A1 (en) * | 2002-04-12 | 2005-08-11 | Chia-Lin Wei | Method for making full-length coding sequence cDNA libraries |
| US6808906B2 (en) | 2002-05-08 | 2004-10-26 | Rigel Pharmaceuticals, Inc. | Directionally cloned random cDNA expression vector libraries, compositions and methods of use |
| US20070178478A1 (en) | 2002-05-08 | 2007-08-02 | Dhallan Ravinder S | Methods for detection of genetic disorders |
| GB0212391D0 (en) | 2002-05-29 | 2002-07-10 | Axis Shield Asa | Assay |
| US20040096368A1 (en) | 2002-06-28 | 2004-05-20 | Igen International, Inc. | Assay systems and components |
| US20050019776A1 (en) | 2002-06-28 | 2005-01-27 | Callow Matthew James | Universal selective genome amplification and universal genotyping system |
| US7192700B2 (en) | 2002-12-20 | 2007-03-20 | Orchid Cellmark Inc. | Methods and compositions for conducting primer extension and polymorphism detection reactions |
| EP2315027A1 (en) | 2002-08-16 | 2011-04-27 | Decision Biomarkers Incorporated | Substrates for isolating, reacting and microscopically analyzing materials |
| AU2003268397A1 (en) | 2002-09-03 | 2004-03-29 | Quanta Biosciences, Inc. | Improved compositions and methods for cdna synthesis |
| US7361821B2 (en) | 2002-09-20 | 2008-04-22 | Intel Corporation | Controlled alignment of nanobarcodes encoding specific information for scanning probe microscopy (SPM) reading |
| DE60229890D1 (de) | 2002-09-30 | 2008-12-24 | Hoffmann La Roche | Oligonukleotide zur genotypisierung des thymidylat-synthase gens |
| WO2004040001A2 (en) | 2002-10-02 | 2004-05-13 | California Institute Of Technology | Microfluidic nucleic acid analysis |
| US7822555B2 (en) | 2002-11-11 | 2010-10-26 | Affymetrix, Inc. | Methods for identifying DNA copy number changes |
| EP1578994A2 (en) | 2002-11-11 | 2005-09-28 | Affymetrix, Inc. | Methods for identifying dna copy number changes |
| US7704687B2 (en) | 2002-11-15 | 2010-04-27 | The Johns Hopkins University | Digital karyotyping |
| JP4395133B2 (ja) | 2002-12-20 | 2010-01-06 | カリパー・ライフ・サイエンシズ・インク． | Ｄｎａの単一分子増幅および検出 |
| US20040214211A1 (en) | 2003-01-23 | 2004-10-28 | U.S. Genomics, Inc. | Methods for analyzing polymer populations |
| US7575865B2 (en) | 2003-01-29 | 2009-08-18 | 454 Life Sciences Corporation | Methods of amplifying and sequencing nucleic acids |
| EP1604040B1 (en) | 2003-03-07 | 2010-10-13 | Rubicon Genomics, Inc. | Amplification and analysis of whole genome and whole transcriptome libraries generated by a dna polymerization process |
| US8206913B1 (en) | 2003-03-07 | 2012-06-26 | Rubicon Genomics, Inc. | Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process |
| WO2006102264A1 (en) | 2005-03-18 | 2006-09-28 | Fluidigm Corporation | Thermal reaction device and method for using the same |
| US7269518B2 (en) | 2003-04-30 | 2007-09-11 | Agilent Technologies, Inc. | Chemical array reading |
| US20040259118A1 (en) | 2003-06-23 | 2004-12-23 | Macevicz Stephen C. | Methods and compositions for nucleic acid sequence analysis |
| US8048627B2 (en) | 2003-07-05 | 2011-11-01 | The Johns Hopkins University | Method and compositions for detection and enumeration of genetic variations |
| US20060281092A1 (en) | 2003-07-24 | 2006-12-14 | Tanja Wille | Method for the reverse transcription and/or amplification of nucleic acids |
| EP1670941A1 (en) | 2003-08-13 | 2006-06-21 | Affymetrix, Inc. | Methods and kits for preparing nucleic acid samples |
| GB0319332D0 (en) | 2003-08-16 | 2003-09-17 | Astrazeneca Ab | Amplification |
| US20050048498A1 (en) | 2003-08-29 | 2005-03-03 | Applera Corporation | Compositions, methods, and kits for assembling probes |
| US7341837B2 (en) | 2003-09-02 | 2008-03-11 | Lawton Robert L | Soluble analyte detection and amplification |
| US7354706B2 (en) | 2003-09-09 | 2008-04-08 | The Regents Of The University Of Colorado, A Body Corporate | Use of photopolymerization for amplification and detection of a molecular recognition event |
| AU2004286201B2 (en) | 2003-09-10 | 2010-09-09 | Altheadx, Inc. | Expression profiling using microarrays |
| WO2005047521A2 (en) | 2003-11-10 | 2005-05-26 | Investigen, Inc. | Methods of preparing nucleic acid for detection |
| WO2005071110A2 (en) | 2004-01-23 | 2005-08-04 | Lingvitae As | Improving polynucleotide ligation reactions |
| WO2005080604A2 (en) | 2004-02-12 | 2005-09-01 | Compass Genetics, Llc | Genetic analysis by sequence-specific sorting |
| US20100216153A1 (en) | 2004-02-27 | 2010-08-26 | Helicos Biosciences Corporation | Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities |
| US7432055B2 (en) | 2004-03-05 | 2008-10-07 | Uchicago Argonne Llc | Dual phase multiplex polymerase chain reaction |
| WO2005111243A2 (en) | 2004-05-07 | 2005-11-24 | Cepheid | Multiplexed detection of biological agents |
| WO2005111242A2 (en) | 2004-05-10 | 2005-11-24 | Parallele Bioscience, Inc. | Digital profiling of polynucleotide populations |
| AU2005252242A1 (en) | 2004-06-07 | 2005-12-22 | Irm Llc | Dispensing systems, software, and related methods |
| US20060035258A1 (en) | 2004-08-06 | 2006-02-16 | Affymetrix, Inc. | Methods for identifying DNA copy number changes |
| US20060041385A1 (en) | 2004-08-18 | 2006-02-23 | Bauer Kenneth D | Method of quantitating proteins and genes in cells using a combination of immunohistochemistry and in situ hybridization |
| US7713697B2 (en) | 2004-08-27 | 2010-05-11 | Gen-Probe Incorporated | Methods and kits for amplifying DNA |
| EP1794173B1 (en) * | 2004-09-10 | 2010-08-04 | Human Genetic Signatures PTY Ltd | Amplification blocker comprising intercalating nucleic acids (ina) containing intercalating pseudonucleotides (ipn) |
| US20060073506A1 (en) | 2004-09-17 | 2006-04-06 | Affymetrix, Inc. | Methods for identifying biological samples |
| US7435084B2 (en) | 2004-12-14 | 2008-10-14 | Align Technology, Inc. | System and methods for casting physical tooth model |
| DK1842226T3 (da) | 2004-11-03 | 2010-10-18 | Iris Molecular Diagnostics Inc | Homogen analytpåvisning |
| EP1836302B1 (en) | 2004-12-23 | 2010-04-14 | Amersham Biosciences Corp. | Ligation-based rna amplification |
| WO2006071770A2 (en) | 2004-12-23 | 2006-07-06 | I-Stat Corporation | Molecular diagnostics system and methods |
| WO2006085616A1 (ja) | 2005-02-10 | 2006-08-17 | Riken | 核酸配列増幅方法 |
| US7407757B2 (en) | 2005-02-10 | 2008-08-05 | Population Genetics Technologies | Genetic analysis by sequence-specific sorting |
| US7393665B2 (en) | 2005-02-10 | 2008-07-01 | Population Genetics Technologies Ltd | Methods and compositions for tagging and identifying polynucleotides |
| JPWO2006093082A1 (ja) * | 2005-03-01 | 2008-08-07 | 和光純薬工業株式会社 | サブトラクション・ポリヌクレオチドの取得方法 |
| WO2006099604A2 (en) | 2005-03-16 | 2006-09-21 | Compass Genetics, Llc | Methods and compositions for assay readouts on multiple analytical platforms |
| US20060263789A1 (en) | 2005-05-19 | 2006-11-23 | Robert Kincaid | Unique identifiers for indicating properties associated with entities to which they are attached, and methods for using |
| US7695886B2 (en) | 2005-05-19 | 2010-04-13 | Fuji Xerox Co., Ltd. | Process for producing resin particle liquid dispersion for electrostatic image developing toner, electrostatic image developing toner and production process thereof |
| US8407013B2 (en) | 2005-06-07 | 2013-03-26 | Peter K. Rogan | AB initio generation of single copy genomic probes |
| US20070065844A1 (en) | 2005-06-08 | 2007-03-22 | Massachusetts Institute Of Technology | Solution-based methods for RNA expression profiling |
| US7796815B2 (en) | 2005-06-10 | 2010-09-14 | The Cleveland Clinic Foundation | Image analysis of biological objects |
| US20070020640A1 (en) | 2005-07-21 | 2007-01-25 | Mccloskey Megan L | Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis |
| US8486621B2 (en) | 2005-08-11 | 2013-07-16 | Cornell Research Foundation, Inc. | Nucleic acid-based matrixes |
| JP2009508495A (ja) | 2005-09-16 | 2009-03-05 | ４５４ ライフ サイエンシーズ コーポレイション | ｃＤＮＡライブラリー調製 |
| WO2007044974A2 (en) | 2005-10-12 | 2007-04-19 | The Research Foundation Of State University Of New York | Absolute pcr quantification |
| US8313711B2 (en) | 2005-11-01 | 2012-11-20 | Freeslate, Inc. | Liquid dispensing for high-throughput experimentation |
| US7375211B2 (en) | 2005-11-18 | 2008-05-20 | Kou Zhong C | Method for detection and quantification of T-cell receptor Vβ repertoire |
| US20080070303A1 (en) | 2005-11-21 | 2008-03-20 | West Michael D | Methods to accelerate the isolation of novel cell strains from pluripotent stem cells and cells obtained thereby |
| US7636465B2 (en) | 2005-12-09 | 2009-12-22 | Cytyc Corporation | Cross-frame object reconstruction for image-based cytology applications |
| WO2007087310A2 (en) | 2006-01-23 | 2007-08-02 | Population Genetics Technologies Ltd. | Nucleic acid analysis using sequence tokens |
| WO2007087312A2 (en) | 2006-01-23 | 2007-08-02 | Population Genetics Technologies Ltd. | Molecular counting |
| US8492098B2 (en) | 2006-02-21 | 2013-07-23 | The Trustees Of Tufts College | Methods and arrays for target analyte detection and determination of reaction components that affect a reaction |
| US20080038727A1 (en) | 2006-03-10 | 2008-02-14 | Applera Corporation | MicroRNA and Messenger RNA Detection on Arrays |
| US7880142B2 (en) | 2006-04-04 | 2011-02-01 | Sidec Technologies Ab | Extended electron tomography |
| US20080194414A1 (en) | 2006-04-24 | 2008-08-14 | Albert Thomas J | Enrichment and sequence analysis of genomic regions |
| WO2007136874A2 (en) | 2006-05-18 | 2007-11-29 | President And Fellows Of Harvard College | Genomic library construction |
| US7833716B2 (en) | 2006-06-06 | 2010-11-16 | Gen-Probe Incorporated | Tagged oligonucleotides and their use in nucleic acid amplification methods |
| WO2007147079A2 (en) | 2006-06-14 | 2007-12-21 | Living Microsystems, Inc. | Rare cell analysis using sample splitting and dna tags |
| EP2077912B1 (en) | 2006-08-07 | 2019-03-27 | The President and Fellows of Harvard College | Fluorocarbon emulsion stabilizing surfactants |
| AU2007284651B2 (en) | 2006-08-09 | 2014-03-20 | Institute For Systems Biology | Organ-specific proteins and methods of their use |
| WO2008147428A1 (en) | 2006-10-05 | 2008-12-04 | Nanopoint, Inc. | Systems and methods for active microfluidic cell handling |
| WO2008057163A2 (en) | 2006-10-09 | 2008-05-15 | Carnegie Mellon University | Preparation of functional gel particles with a dual crosslink network |
| SK50302009A3 (sk) | 2006-10-18 | 2010-03-08 | Osaki Electric Co., Ltd. | Elektronický watthodinový elektromer |
| US20100184152A1 (en) | 2006-10-23 | 2010-07-22 | Vladislav Sandler | Target-oriented whole genome amplification of nucleic acids |
| CA2669728C (en) | 2006-11-15 | 2017-04-11 | Biospherex Llc | Multitag sequencing and ecogenomics analysis |
| US8050476B2 (en) | 2006-11-22 | 2011-11-01 | General Electric Company | Heart rate demodulation of periodic movement in ultrasound data for object identification |
| GB2457851B (en) | 2006-12-14 | 2011-01-05 | Ion Torrent Systems Inc | Methods and apparatus for measuring analytes using large scale fet arrays |
| WO2008087869A1 (ja) | 2007-01-16 | 2008-07-24 | Olympus Corporation | 蛍光信号解析装置および蛍光信号解析方法 |
| US9063133B2 (en) | 2007-01-30 | 2015-06-23 | The Regents Of The University Of California | Methods and devices for biomolecular arrays |
| US20080269068A1 (en) | 2007-02-06 | 2008-10-30 | President And Fellows Of Harvard College | Multiplex decoding of sequence tags in barcodes |
| WO2008096318A2 (en) | 2007-02-09 | 2008-08-14 | Koninklijke Philips Electronics N.V. | Identification system |
| US8003312B2 (en) | 2007-02-16 | 2011-08-23 | The Board Of Trustees Of The Leland Stanford Junior University | Multiplex cellular assays using detectable cell barcodes |
| BRPI0807384A2 (pt) | 2007-02-27 | 2014-05-20 | Sentoclone Ab | Método de uma etapa para detecção direta ou simultânea de uma célula cancerosa ou elemento de célula cancerosa em uma amostra biológica, kit para uso em um método, base de dados, método para indetificar células cancerosas, e, uso de uma base de dados. |
| US9029085B2 (en) | 2007-03-07 | 2015-05-12 | President And Fellows Of Harvard College | Assays and other reactions involving droplets |
| KR100882711B1 (ko) | 2007-03-12 | 2009-02-06 | 성균관대학교산학협력단 | 사이크로박터 스피시스 ｈｊ147 균주 유래의 우라실-ｄｎａ글리코실라제 및 이의 용도 |
| JP2008256428A (ja) | 2007-04-03 | 2008-10-23 | Intec Systems Institute Inc | マイクロアレイ画像のブロック位置検出方法 |
| US20080268508A1 (en) | 2007-04-30 | 2008-10-30 | Sowlay Mohankumar R | Methods and kits for negative selection of desired nucleic acid sequences |
| US20080274458A1 (en) | 2007-05-01 | 2008-11-06 | Latham Gary J | Nucleic acid quantitation methods |
| US20090061513A1 (en) | 2007-05-15 | 2009-03-05 | Picovitro Ab | Cell sorting and cell cultivation methods |
| JP2010528608A (ja) | 2007-06-01 | 2010-08-26 | ４５４ ライフ サイエンシーズ コーポレイション | 複合的な混合物から個々の試料を特定するためのシステムおよび方法 |
| EP2171097A2 (en) | 2007-06-29 | 2010-04-07 | Population Genetics Technologies LTD. | Methods and compositions for isolating nucleic acid sequence variants |
| EP2036989B1 (en) | 2007-09-12 | 2012-07-25 | Institut Pasteur | Polynucleotide suitable for single cell based reporter assay to monitor gene expression patterns with high spatio-temporal resolution |
| EP3136103B1 (en) | 2007-10-05 | 2018-08-29 | Affymetrix, Inc. | Highly multiplexed assays |
| WO2009105670A2 (en) | 2008-02-21 | 2009-08-27 | Gentel Biosciences, Inc. | Substrates for multiplexed assays and uses thereof |
| US8687189B2 (en) | 2008-03-03 | 2014-04-01 | Ajjer, Llc | Analysis of arrays by laser induced breakdown spectroscopy |
| US8206925B2 (en) | 2008-04-14 | 2012-06-26 | Medical Diagnostic Laboratories, Llc | Splice variants of human IL-23 receptor (IL-23R) mRNA and use of a delta 9 isoform in predicting inflammatory bowel diseases |
| DE102008025656B4 (de) | 2008-05-28 | 2016-07-28 | Genxpro Gmbh | Verfahren zur quantitativen Analyse von Nukleinsäuren, Marker dafür und deren Verwendung |
| US20090298709A1 (en) | 2008-05-28 | 2009-12-03 | Affymetrix, Inc. | Assays for determining telomere length and repeated sequence copy number |
| WO2009148560A2 (en) | 2008-05-30 | 2009-12-10 | Board Of Regents, The Universtiy Of Texas System | Methods and compositions for nucleic acid sequencing |
| US20100069250A1 (en) | 2008-08-16 | 2010-03-18 | The Board Of Trustees Of The Leland Stanford Junior University | Digital PCR Calibration for High Throughput Sequencing |
| US8476013B2 (en) | 2008-09-16 | 2013-07-02 | Sequenom, Inc. | Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses |
| US9080211B2 (en) | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| WO2010053980A2 (en) | 2008-11-04 | 2010-05-14 | The Johns Hopkins University | Dna integrity assay (dia) for cancer diagnostics, using confocal fluorescence spectroscopy |
| US8993245B2 (en) | 2008-11-21 | 2015-03-31 | Mediomics, Llc | Biosensor for detecting multiple epitopes on a target |
| US20100159533A1 (en) | 2008-11-24 | 2010-06-24 | Helicos Biosciences Corporation | Simplified sample preparation for rna analysis |
| US8492096B2 (en) | 2008-12-24 | 2013-07-23 | Boris Pasche | TGFBR1 expression modifies risk for colorectal cancer |
| AU2010206843B2 (en) | 2009-01-20 | 2015-01-29 | The Board Of Trustees Of The Leland Stanford Junior University | Single cell gene expression for diagnosis, prognosis and identification of drug targets |
| US20100323348A1 (en) | 2009-01-31 | 2010-12-23 | The Regents Of The University Of Colorado, A Body Corporate | Methods and Compositions for Using Error-Detecting and/or Error-Correcting Barcodes in Nucleic Acid Amplification Process |
| CA2754446A1 (en) | 2009-03-05 | 2010-09-10 | Daiichi Sankyo Company, Limited | Pyridine derivative |
| EP2230312A1 (en) | 2009-03-19 | 2010-09-22 | Helmholtz-Zentrum für Infektionsforschung GmbH | Probe compound for detecting and isolating enzymes and means and methods using the same |
| EP2414548B1 (en) | 2009-03-30 | 2015-10-21 | Illumina, Inc. | Gene expression analysis in single cells |
| SG174617A1 (en) | 2009-04-02 | 2011-10-28 | Fluidigm Corp | Multi-primer amplification method for barcoding of target nucleic acids |
| US9085798B2 (en) | 2009-04-30 | 2015-07-21 | Prognosys Biosciences, Inc. | Nucleic acid constructs and methods of use |
| FR2945545B1 (fr) | 2009-05-14 | 2011-08-05 | Univ Aix Marseille Ii | Methode de detection d'adn procaryote extrait d'un echantillon de selles |
| WO2010131645A1 (ja) | 2009-05-14 | 2010-11-18 | 和光純薬工業株式会社 | Rnaに対応する二本鎖dnaの合成方法及び増幅方法 |
| US8673627B2 (en) | 2009-05-29 | 2014-03-18 | Life Technologies Corporation | Apparatus and methods for performing electrochemical reactions |
| GB0909923D0 (en) | 2009-06-09 | 2009-07-22 | Oxford Gene Tech Ip Ltd | Picowell capture devices for analysing single cells or other particles |
| US20120058902A1 (en) | 2009-06-25 | 2012-03-08 | Livingston Robert J | Method of measuring adaptive immunity |
| US8330957B2 (en) | 2009-06-29 | 2012-12-11 | Hager Enviromental and Atmospheric Technologies, LLC | Device and method for quantification of gases in plumes by remote sensing |
| US9587270B2 (en) | 2009-06-29 | 2017-03-07 | Luminex Corporation | Chimeric primers with hairpin conformations and methods of using same |
| EP3029141A1 (en) | 2009-08-20 | 2016-06-08 | Population Genetics Technologies Ltd. | Compositions and methods for intramolecular nucleic acid rearrangement |
| WO2011028818A2 (en) | 2009-09-01 | 2011-03-10 | Trustees Of Boston University | High throughput multichannel reader and uses thereof |
| WO2011027268A2 (en) | 2009-09-01 | 2011-03-10 | Koninklijke Philips Electronics N.V. | Devices and methods for microarray selection |
| US9625454B2 (en) | 2009-09-04 | 2017-04-18 | The Research Foundation For The State University Of New York | Rapid and continuous analyte processing in droplet microfluidic devices |
| WO2011041308A1 (en) | 2009-09-30 | 2011-04-07 | Quest Diagnostics Investments Incorporated | Bcr-abl truncation mutations |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US9315857B2 (en) | 2009-12-15 | 2016-04-19 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse label-tags |
| EP2513341B1 (en) | 2010-01-19 | 2017-04-12 | Verinata Health, Inc | Identification of polymorphic sequences in mixtures of genomic dna by whole genome sequencing |
| EP2370599B1 (en) | 2010-01-19 | 2015-01-21 | Verinata Health, Inc | Method for determining copy number variations |
| CN102725424B (zh) | 2010-01-25 | 2014-07-09 | Rd生物科技公司 | 靶核酸的自折叠扩增 |
| EP4484577A3 (en) | 2010-02-12 | 2025-03-26 | Bio-Rad Laboratories, Inc. | Digital analyte analysis |
| JP5901046B2 (ja) | 2010-02-19 | 2016-04-06 | 国立大学法人 千葉大学 | ＯＡＴＰ１Ｂ３ｍＲＮＡの新規な選択的スプライシングバリアント |
| WO2011106738A2 (en) | 2010-02-25 | 2011-09-01 | Fred Hutchinson Cancer Research Center | Use of tcr clonotypes as biomarkers for disease |
| US8951940B2 (en) | 2010-04-01 | 2015-02-10 | Illumina, Inc. | Solid-phase clonal amplification and related methods |
| WO2011127099A1 (en) | 2010-04-05 | 2011-10-13 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| WO2011143659A2 (en) | 2010-05-14 | 2011-11-17 | Fluidigm Corporation | Nucleic acid isolation methods |
| US8828688B2 (en) | 2010-05-27 | 2014-09-09 | Affymetrix, Inc. | Multiplex amplification methods |
| US9080210B2 (en) | 2010-06-09 | 2015-07-14 | Keygene N.V. | High throughput screening using combinatorial sequence barcodes |
| US20120004132A1 (en) | 2010-07-02 | 2012-01-05 | Affymetrix, Inc. | Detection of Nucleic Acids and Proteins |
| WO2012004834A1 (ja) | 2010-07-07 | 2012-01-12 | 株式会社アドバンテスト | 試験装置および試験方法 |
| EP2407242A1 (en) | 2010-07-13 | 2012-01-18 | Dublin City University | Direct clone analysis and selection technology |
| US20120040843A1 (en) | 2010-08-12 | 2012-02-16 | Dublin City University | Centrifugal capture system |
| ES2523140T3 (es) | 2010-09-21 | 2014-11-21 | Population Genetics Technologies Ltd. | Aumento de la confianza en las identificaciones de alelos con el recuento molecular |
| EP2436766A1 (en) | 2010-09-29 | 2012-04-04 | Deutsches Krebsforschungszentrum | Means and methods for improved protein interaction screening |
| WO2012042374A2 (en) | 2010-10-01 | 2012-04-05 | Anssi Jussi Nikolai Taipale | Method of determining number or concentration of molecules |
| US20120088691A1 (en) | 2010-10-08 | 2012-04-12 | Gao Chen | Highly multiplexed real-time pcr using encoded microbeads |
| GB2497912B (en) | 2010-10-08 | 2014-06-04 | Harvard College | High-throughput single cell barcoding |
| US20130225623A1 (en) | 2010-10-27 | 2013-08-29 | Mount Sinai School Of Medicine | Methods of Treating Psychiatric or Neurological Disorders with MGLUR Antagonists |
| WO2012075028A1 (en) | 2010-11-29 | 2012-06-07 | Dako Denmark A/S | Methods and systems for analyzing images of specimens processed by a programmable quantitative assay |
| US9394511B2 (en) | 2010-12-05 | 2016-07-19 | Wenbin Jiang | Rapid single cell based parallel biological cell sorter |
| CN107159328B (zh) | 2010-12-09 | 2020-04-14 | 阿科尼生物系统公司 | 样本分析系统 |
| WO2012083225A2 (en) | 2010-12-16 | 2012-06-21 | Gigagen, Inc. | System and methods for massively parallel analysis of nycleic acids in single cells |
| CN103608466B (zh) | 2010-12-22 | 2020-09-18 | 纳特拉公司 | 非侵入性产前亲子鉴定方法 |
| US10241075B2 (en) | 2010-12-30 | 2019-03-26 | Life Technologies Corporation | Methods, systems, and computer readable media for nucleic acid sequencing |
| EP3567121B1 (en) | 2011-01-17 | 2023-08-30 | Life Technologies Corporation | Workflow for detection of ligands using nucleic acids |
| WO2012103154A1 (en) | 2011-01-24 | 2012-08-02 | Nugen Technologies, Inc. | Stem-loop composite rna-dna adaptor-primers: compositions and methods for library generation, amplification and other downstream manipulations |
| US20120190020A1 (en) | 2011-01-25 | 2012-07-26 | Aria Diagnostics, Inc. | Detection of genetic abnormalities |
| WO2016100976A2 (en) | 2014-12-19 | 2016-06-23 | Apprise Bio, Inc. | Methods for identifying multiple epitopes in selected sub-populations of cells |
| US9365897B2 (en) * | 2011-02-08 | 2016-06-14 | Illumina, Inc. | Selective enrichment of nucleic acids |
| WO2012112804A1 (en) | 2011-02-18 | 2012-08-23 | Raindance Technoligies, Inc. | Compositions and methods for molecular labeling |
| AU2012220596B2 (en) | 2011-02-23 | 2016-05-12 | Board Of Regents | Use of template switching for DNA synthesis |
| WO2012129363A2 (en) | 2011-03-24 | 2012-09-27 | President And Fellows Of Harvard College | Single cell nucleic acid detection and analysis |
| KR20120110431A (ko) | 2011-03-29 | 2012-10-10 | 에스케이하이닉스 주식회사 | 반도체 메모리 장치 |
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| ES2986436T3 (es) | 2011-04-15 | 2024-11-11 | Univ Johns Hopkins | Sistema de secuenciación segura |
| EP3395957B1 (en) | 2011-04-25 | 2020-08-12 | Bio-Rad Laboratories, Inc. | Methods and compositions for nucleic acid analysis |
| CN103732738A (zh) | 2011-04-28 | 2014-04-16 | 小利兰斯坦福大学托管委员会 | 与样品相关的多核苷酸的鉴定 |
| GB201108259D0 (en) | 2011-05-17 | 2011-06-29 | Cambridge Entpr Ltd | Gel beads in microfluidic droplets |
| SG10201605049QA (en) | 2011-05-20 | 2016-07-28 | Fluidigm Corp | Nucleic acid encoding reactions |
| PL3892295T3 (pl) | 2011-05-24 | 2023-07-24 | BioNTech SE | Zindywidualizowane szczepionki przeciwnowotworowe |
| WO2012162621A1 (en) | 2011-05-26 | 2012-11-29 | Brandeis University | Methods for suppression pcr |
| US8841071B2 (en) | 2011-06-02 | 2014-09-23 | Raindance Technologies, Inc. | Sample multiplexing |
| US9752176B2 (en) | 2011-06-15 | 2017-09-05 | Ginkgo Bioworks, Inc. | Methods for preparative in vitro cloning |
| WO2012177639A2 (en) | 2011-06-22 | 2012-12-27 | Alnylam Pharmaceuticals, Inc. | Bioprocessing and bioproduction using avian cell lines |
| EP2724160A2 (en) | 2011-06-27 | 2014-04-30 | Life Technologies Corporation | Acoustic cytometry methods and protocols |
| KR101454886B1 (ko) | 2011-08-01 | 2014-11-03 | 주식회사 셀레믹스 | 핵산분자의 제조방법 |
| US10364464B2 (en) | 2011-08-08 | 2019-07-30 | The Broad Institute, Inc. | Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence |
| JP5816486B2 (ja) | 2011-08-18 | 2015-11-18 | オリンパス株式会社 | 蛍光観察装置および蛍光観察システム並びに蛍光観察装置の蛍光画像処理方法 |
| LT2756098T (lt) | 2011-09-16 | 2018-09-10 | Lexogen Gmbh | Nukleorūgščių molekulių bibliotekos sudarymo būdas |
| US9297047B2 (en) | 2011-10-24 | 2016-03-29 | Jennifer Furchak | Molecular beacon based assay for the detection of biomarkers for breast cancer metastasis |
| EP2776833B1 (en) | 2011-11-11 | 2018-09-05 | Eli N. Glezer | Co-binder assisted assay methods |
| KR101337094B1 (ko) | 2011-11-30 | 2013-12-05 | 삼성에스디에스 주식회사 | 염기 서열 정렬 장치 및 그 방법 |
| WO2013096802A1 (en) | 2011-12-22 | 2013-06-27 | Ibis Biosciences, Inc. | Amplification primers and methods |
| WO2013117595A2 (en) | 2012-02-07 | 2013-08-15 | Illumina Cambridge Limited | Targeted enrichment and amplification of nucleic acids on a support |
| WO2013120089A1 (en) | 2012-02-10 | 2013-08-15 | Raindance Technologies, Inc. | Molecular diagnostic screening assay |
| US10202628B2 (en) | 2012-02-17 | 2019-02-12 | President And Fellows Of Harvard College | Assembly of nucleic acid sequences in emulsions |
| EP3309262B1 (en) | 2012-02-24 | 2019-09-25 | Bio-Rad Laboratories, Inc. | Labeling and sample preparation for sequencing |
| EP2820174B1 (en) | 2012-02-27 | 2019-12-25 | The University of North Carolina at Chapel Hill | Methods and uses for molecular tags |
| CA2865575C (en) | 2012-02-27 | 2024-01-16 | Cellular Research, Inc. | Compositions and kits for molecular counting |
| WO2013137737A1 (en) | 2012-03-16 | 2013-09-19 | Flexgen B.V. | Methods of creating and screening of dna encoded combinatorial libraries of chemical antibodies |
| US9803239B2 (en) | 2012-03-29 | 2017-10-31 | Complete Genomics, Inc. | Flow cells for high density array chips |
| EP2647426A1 (en) | 2012-04-03 | 2013-10-09 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Replication of distributed nucleic acid molecules with preservation of their relative distribution through hybridization-based binding |
| HUE029357T2 (en) | 2012-05-08 | 2017-02-28 | Adaptive Biotechnologies Corp | Preparations and devices for measuring and calibrating amplification distortion in multiplex PCR reactions |
| PT2850211T (pt) | 2012-05-14 | 2021-11-29 | Irepertoire Inc | Método para aumentar a precisão na deteção quantitativa de polinucleótidos |
| WO2013177206A2 (en) | 2012-05-21 | 2013-11-28 | Fluidigm Corporation | Single-particle analysis of particle populations |
| JP6200948B2 (ja) | 2012-05-25 | 2017-09-20 | ザ・ユニヴァーシティ・オヴ・ノース・キャロライナ・アト・チャペル・ヒル | マイクロ流体デバイス |
| EP2861760B1 (en) | 2012-06-15 | 2020-01-01 | The Board of Regents of The University of Texas System | High throughput sequencing of multiple transcripts of a single cell |
| SG11201408128WA (en) | 2012-06-15 | 2015-01-29 | Adaptive Biotechnologies Corp | Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set |
| CA2877094A1 (en) * | 2012-06-18 | 2013-12-27 | Nugen Technologies, Inc. | Compositions and methods for negative selection of non-desired nucleic acid sequences |
| CA2876505A1 (en) | 2012-07-17 | 2014-01-23 | Counsyl, Inc. | System and methods for detecting genetic variation |
| WO2014015098A1 (en) * | 2012-07-18 | 2014-01-23 | Siemens Healthcare Diagnostics Inc. | A method of normalizing biological samples |
| EP2877604A1 (en) | 2012-07-24 | 2015-06-03 | Sequenta, Inc. | Single cell analysis using sequence tags |
| US10870099B2 (en) | 2012-07-26 | 2020-12-22 | Illumina, Inc. | Compositions and methods for the amplification of nucleic acids |
| EP2882868B1 (en) | 2012-08-08 | 2019-07-31 | H. Hoffnabb-La Roche Ag | Increasing dynamic range for identifying multiple epitopes in cells |
| US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| AU2013302756C1 (en) | 2012-08-14 | 2018-05-17 | 10X Genomics, Inc. | Microcapsule compositions and methods |
| US20140378322A1 (en) | 2012-08-14 | 2014-12-25 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US20150005200A1 (en) | 2012-08-14 | 2015-01-01 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| US20140378349A1 (en) | 2012-08-14 | 2014-12-25 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| US20140378345A1 (en) | 2012-08-14 | 2014-12-25 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| US20150005199A1 (en) | 2012-08-14 | 2015-01-01 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| EP4148118A1 (en) | 2012-08-24 | 2023-03-15 | Yale University | System, device and method for high-throughput multi-plexed detection |
| EP4592400A3 (en) | 2012-10-17 | 2025-10-29 | 10x Genomics Sweden AB | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| WO2015160439A2 (en) | 2014-04-17 | 2015-10-22 | Adaptive Biotechnologies Corporation | Quantification of adaptive immune cell genomes in a complex mixture of cells |
| GB201218909D0 (en) | 2012-10-22 | 2012-12-05 | Univ Singapore | Assay for the parallel detection of biological material based on PCR |
| WO2014071361A1 (en) | 2012-11-05 | 2014-05-08 | Rubicon Genomics | Barcoding nucleic acids |
| CA2894694C (en) | 2012-12-14 | 2023-04-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9683230B2 (en) | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
| WO2014108850A2 (en) | 2013-01-09 | 2014-07-17 | Yeda Research And Development Co. Ltd. | High throughput transcriptome analysis |
| US9562269B2 (en) | 2013-01-22 | 2017-02-07 | The Board Of Trustees Of The Leland Stanford Junior University | Haplotying of HLA loci with ultra-deep shotgun sequencing |
| CN104955576A (zh) | 2013-01-24 | 2015-09-30 | 沙特基础全球技术有限公司 | 由聚酯-聚碳酸酯制成的微孔板 |
| US10017761B2 (en) | 2013-01-28 | 2018-07-10 | Yale University | Methods for preparing cDNA from low quantities of cells |
| US10190963B2 (en) | 2013-02-01 | 2019-01-29 | Becton, Dickinson And Company | Methods and systems for assessing sample behavior in a flow cytometer |
| KR20200140929A (ko) | 2013-02-08 | 2020-12-16 | 10엑스 제노믹스, 인크. | 폴리뉴클레오티드 바코드 생성 |
| CN105102696B (zh) | 2013-02-08 | 2017-08-15 | 生物辐射实验室股份有限公司 | 用于目标分子检测的基于亲和力的划分试验 |
| WO2014126937A1 (en) | 2013-02-14 | 2014-08-21 | The Board Of Trustees Of The Leland Stanford Junior University | Suspension arrays and multiplexed assays based thereon |
| US9621600B2 (en) | 2013-02-26 | 2017-04-11 | PortAura Group | Method and system for providing recommendations using location information |
| US20140274811A1 (en) | 2013-03-14 | 2014-09-18 | Lyle J. Arnold | Methods for Amplifying a Complete Genome or Transcriptome |
| US9938523B2 (en) | 2013-03-15 | 2018-04-10 | Arizona Board Of Regents On Behalf Of Arizona State University | Nucleic acid-tagged compositions and methods for multiplexed protein-protein interaction profiling |
| US9328382B2 (en) | 2013-03-15 | 2016-05-03 | Complete Genomics, Inc. | Multiple tagging of individual long DNA fragments |
| EP3611262B1 (en) | 2013-03-15 | 2020-11-11 | Lineage Biosciences, Inc. | Methods of sequencing the immune repertoire |
| US9255265B2 (en) | 2013-03-15 | 2016-02-09 | Illumina, Inc. | Methods for producing stranded cDNA libraries |
| GB2525568B (en) | 2013-03-15 | 2020-10-14 | Abvitro Llc | Single cell barcoding for antibody discovery |
| US20140303005A1 (en) | 2013-04-05 | 2014-10-09 | Raindance Technologies, Inc. | Rare cell analysis after negative selection |
| SG10201805453XA (en) | 2013-04-25 | 2018-08-30 | Firefly Bioworks Inc | Multiplexed analysis of target nucleic acids |
| EP2805769A1 (en) | 2013-05-24 | 2014-11-26 | European Molecular Biology Laboratory | Methods for nano-scale single cell analysis |
| ES2754177T3 (es) | 2013-06-12 | 2020-04-16 | Massachusetts Gen Hospital | Métodos para la detección multiplexada de moléculas diana y sus usos |
| WO2014201273A1 (en) | 2013-06-12 | 2014-12-18 | The Broad Institute, Inc. | High-throughput rna-seq |
| US20150011398A1 (en) | 2013-06-17 | 2015-01-08 | Kim Lewis | Methods for quantitative determination of protein-nucleic acid interactions in complex mixtures |
| CA2916662C (en) | 2013-06-25 | 2022-03-08 | Prognosys Biosciences, Inc. | Methods and systems for determining spatial patterns of biological targets in a sample |
| AU2014302277A1 (en) | 2013-06-27 | 2015-12-24 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US9708657B2 (en) | 2013-07-01 | 2017-07-18 | Adaptive Biotechnologies Corp. | Method for generating clonotype profiles using sequence tags |
| KR102758333B1 (ko) | 2013-08-28 | 2025-01-23 | 벡톤 디킨슨 앤드 컴퍼니 | 대량의 동시 단일 세포 분석 |
| US10395758B2 (en) | 2013-08-30 | 2019-08-27 | 10X Genomics, Inc. | Sequencing methods |
| EP3041957A4 (en) | 2013-09-04 | 2017-03-29 | Fluidigm Corporation | Proximity assays for detecting nucleic acids and proteins in a single cell |
| GB201317301D0 (en) | 2013-09-30 | 2013-11-13 | Linnarsson Sten | Method for capturing and encoding nucleic acid from a plurality of single cells |
| CN105745334B (zh) | 2013-09-30 | 2019-12-10 | 吴迪 | 通过使用邻近条形编码分析分子复合物的概况的方法 |
| US9582877B2 (en) | 2013-10-07 | 2017-02-28 | Cellular Research, Inc. | Methods and systems for digitally counting features on arrays |
| EP3875601A1 (en) | 2013-10-17 | 2021-09-08 | Illumina, Inc. | Methods and compositions for preparing nucleic acid libraries |
| WO2015061844A1 (en) | 2013-10-30 | 2015-05-07 | The University Of Sydney | Assay to stratify cancer patients |
| US10288608B2 (en) | 2013-11-08 | 2019-05-14 | Prognosys Biosciences, Inc. | Polynucleotide conjugates and methods for analyte detection |
| DK3089822T3 (da) | 2013-12-30 | 2022-05-02 | Atreca Inc | Analyse af nukleinsyrer associeret med enkelte celler under anvendelse af nukleinsyrestregkoder |
| EP3099820A4 (en) | 2014-01-27 | 2018-01-03 | The General Hospital Corporation | Methods of preparing nucleic acids for sequencing |
| EP3102678A2 (en) | 2014-02-03 | 2016-12-14 | Integrated DNA Technologies Inc. | Methods to capture and/or remove highly abundant rnas from a heterogeneous rna sample |
| WO2015134787A2 (en) | 2014-03-05 | 2015-09-11 | Adaptive Biotechnologies Corporation | Methods using randomer-containing synthetic molecules |
| US20150298091A1 (en) | 2014-04-21 | 2015-10-22 | President And Fellows Of Harvard College | Systems and methods for barcoding nucleic acids |
| US10975371B2 (en) | 2014-04-29 | 2021-04-13 | Illumina, Inc. | Nucleic acid sequence analysis from single cells |
| CA3060708A1 (en) | 2014-04-29 | 2015-11-05 | Illumina, Inc | Multiplexed single cell gene expression analysis using template switch and tagmentation |
| WO2015179339A1 (en) | 2014-05-19 | 2015-11-26 | William Marsh Rice University | Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides |
| WO2015200541A1 (en) | 2014-06-24 | 2015-12-30 | Bio-Rad Laboratories, Inc. | Digital pcr barcoding |
| WO2015200893A2 (en) | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
| CA2953469A1 (en) | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| EP3169799B1 (en) | 2014-07-15 | 2019-09-04 | Qiagen Sciences, LLC | Semi-random barcodes for nucleic acid analysis |
| WO2016040446A1 (en) | 2014-09-10 | 2016-03-17 | Good Start Genetics, Inc. | Methods for selectively suppressing non-target sequences |
| EP3950944A1 (en) | 2014-09-15 | 2022-02-09 | AbVitro LLC | High-throughput nucleotide library sequencing |
| US9529672B2 (en) | 2014-09-25 | 2016-12-27 | Everspin Technologies Inc. | ECC word configuration for system-level ECC compatibility |
| RU2736728C2 (ru) | 2014-10-17 | 2020-11-19 | Иллумина Кембридж Лимитед | Транспозиция с сохранением сцепления генов |
| EP3248018B1 (en) | 2015-01-22 | 2020-01-08 | Becton, Dickinson and Company | Devices and systems for molecular barcoding of nucleic acid targets in single cells |
| US10760120B2 (en) | 2015-01-23 | 2020-09-01 | Qiagen Sciences, Llc | High multiplex PCR with molecular barcoding |
| WO2016130578A1 (en) | 2015-02-09 | 2016-08-18 | 10X Genomics, Inc. | Systems and methods for determining structural variation and phasing using variant call data |
| EP3259371B1 (en) | 2015-02-19 | 2020-09-02 | Becton, Dickinson and Company | High-throughput single-cell analysis combining proteomic and genomic information |
| WO2016138496A1 (en) | 2015-02-27 | 2016-09-01 | Cellular Research, Inc. | Spatially addressable molecular barcoding |
| WO2016138500A1 (en) | 2015-02-27 | 2016-09-01 | Cellular Research, Inc. | Methods and compositions for barcoding nucleic acids for sequencing |
| US11873483B2 (en) | 2015-03-11 | 2024-01-16 | The Broad Institute, Inc. | Proteomic analysis with nucleic acid identifiers |
| US20180073073A1 (en) | 2015-03-18 | 2018-03-15 | Cellular Research, Inc. | Methods and compositions for labeling targets and haplotype phasing |
| WO2016160844A2 (en) | 2015-03-30 | 2016-10-06 | Cellular Research, Inc. | Methods and compositions for combinatorial barcoding |
| US10301660B2 (en) | 2015-03-30 | 2019-05-28 | Takara Bio Usa, Inc. | Methods and compositions for repair of DNA ends by multiple enzymatic activities |
| WO2016168825A1 (en) | 2015-04-17 | 2016-10-20 | Centrillion Technology Holdings Corporation | Methods for performing spatial profiling of biological molecules |
| EP4582556A3 (en) | 2015-04-23 | 2025-10-08 | Becton, Dickinson and Company | Methods and compositions for whole transcriptome amplification |
| US9618871B2 (en) | 2015-04-28 | 2017-04-11 | Kyocera Document Solutions Inc. | Image forming apparatus |
| JP2018516569A (ja) | 2015-05-22 | 2018-06-28 | シグマ−アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニーＳｉｇｍａ−Ａｌｄｒｉｃｈ Ｃｏ．， ＬＬＣ | 次世代ゲノムウォーキングのための方法ならびに関連する組成物およびキット |
| WO2016190795A1 (en) | 2015-05-28 | 2016-12-01 | Kaarel Krjutskov | Blocking oligonucleotides |
| WO2016196229A1 (en) | 2015-06-01 | 2016-12-08 | Cellular Research, Inc. | Methods for rna quantification |
| CN107849561A (zh) | 2015-08-24 | 2018-03-27 | 凯杰有限公司 | 产生rna测序文库的方法 |
| EP4086357A1 (en) | 2015-08-28 | 2022-11-09 | Illumina, Inc. | Nucleic acid sequence analysis from single cells |
| JP6940484B2 (ja) | 2015-09-11 | 2021-09-29 | セルラー リサーチ， インコーポレイテッド | ライブラリー正規化のための方法および組成物 |
| MX2018003532A (es) | 2015-09-24 | 2019-04-25 | Abvitro Llc | Conjugados de afinidad-oligonucleotido y usos de los mismos. |
| US11092607B2 (en) | 2015-10-28 | 2021-08-17 | The Board Institute, Inc. | Multiplex analysis of single cell constituents |
| JP7064439B2 (ja) | 2015-11-04 | 2022-05-10 | アトレカ インコーポレイテッド | 単一細胞に関連する核酸の解析のための、核酸バーコードの組み合わせセット |
| WO2017087873A1 (en) | 2015-11-18 | 2017-05-26 | Wafergen, Inc. | Systems and methods for pooling samples from multi-well devices |
| US12297427B2 (en) | 2015-12-04 | 2025-05-13 | St. Jude Children's Research Hospital, Inc. | Cloning and expression system for T-cell receptors |
| CN108779492A (zh) | 2015-12-30 | 2018-11-09 | 生物辐射实验室股份有限公司 | 数字蛋白质定量 |
| US20170205404A1 (en) | 2016-01-19 | 2017-07-20 | General Electric Company | Multifunctional beads and methods of use for capturing rare cells |
| US20170260584A1 (en) | 2016-02-11 | 2017-09-14 | 10X Genomics, Inc. | Cell population analysis using single nucleotide polymorphisms from single cell transcriptomes |
| US12060412B2 (en) | 2016-03-21 | 2024-08-13 | The Broad Institute, Inc. | Methods for determining spatial and temporal gene expression dynamics in single cells |
| WO2017173328A1 (en) | 2016-04-01 | 2017-10-05 | Baylor College Of Medicine | Methods of whole transcriptome amplification |
| JP7129343B2 (ja) | 2016-05-02 | 2022-09-01 | ベクトン・ディキンソン・アンド・カンパニー | 正確な分子バーコーディング |
| WO2017196768A1 (en) | 2016-05-09 | 2017-11-16 | President And Fellows Of Harvard College | Self-targeting guide rnas in crispr system |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| ES2979395T3 (es) | 2016-05-26 | 2024-09-25 | Becton Dickinson Co | Métodos de ajuste del recuento de etiquetas moleculares |
| TWI600309B (zh) | 2016-05-28 | 2017-09-21 | Hon Hai Prec Ind Co Ltd | 角度調整機構 |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| EP3686287A1 (en) | 2016-06-28 | 2020-07-29 | Hifibio | Method for transcriptome analysis of single cells |
| EP4717780A2 (en) | 2016-07-22 | 2026-04-01 | Verily Life Sciences LLC | Quantitative massively parallel proteomics |
| US20190203270A1 (en) | 2016-07-24 | 2019-07-04 | Yeda Research And Development Co., Ltd. | Methods and kits for analyzing dna binding moieties attached to dna |
| US20180037942A1 (en) | 2016-08-03 | 2018-02-08 | Cellular Research, Inc. | Enzyme-independent molecular indexing |
| AU2017311306A1 (en) | 2016-08-10 | 2019-03-14 | President And Fellows Of Harvard College | Methods of de novo assembly of barcoded genomic DNA fragments |
| CN109791157B (zh) | 2016-09-26 | 2022-06-07 | 贝克顿迪金森公司 | 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达 |
| CN109804079A (zh) | 2016-09-29 | 2019-05-24 | 富士胶片株式会社 | 供多重pcr的引物的设计方法 |
| WO2018061695A1 (ja) | 2016-09-29 | 2018-04-05 | 富士フイルム株式会社 | マルチプレックスｐｃｒに供するプライマーの設計方法 |
| CA3038535A1 (en) | 2016-10-01 | 2018-04-05 | Berkeley Lights, Inc. | Dna barcode compositions and methods of in situ identification in a microfluidic device |
| DK3529357T3 (da) | 2016-10-19 | 2022-04-25 | 10X Genomics Inc | Fremgangsmåder til stregkodning af nukleinsyremolekyler fra individuelle celler |
| EP3551768B1 (en) | 2016-12-12 | 2024-03-06 | Grail, LLC | Methods for tagging and amplifying rna template molecules for preparing sequencing libraries |
| US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US20190177800A1 (en) | 2017-12-08 | 2019-06-13 | 10X Genomics, Inc. | Methods and compositions for labeling cells |
| GB201622222D0 (en) | 2016-12-23 | 2017-02-08 | Cs Genetics Ltd | Reagents and methods for molecular barcoding of nucleic acids of single cells |
| EP3568508A4 (en) | 2017-01-12 | 2020-12-16 | Massachusetts Institute Of Technology | METHODS FOR T-LYMPHOCYTE RECEPTOR AND B-LYMPHOCYTE RECEPTOR ANALYSIS |
| US20180208975A1 (en) | 2017-01-20 | 2018-07-26 | Merck Sharp & Dohme Corp. | Assay for simultaneous genomic and proteomic analysis |
| EP4029939B1 (en) | 2017-01-30 | 2023-06-28 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
| CN110382708A (zh) | 2017-02-01 | 2019-10-25 | 赛卢拉研究公司 | 使用阻断性寡核苷酸进行选择性扩增 |
| CN117887804A (zh) | 2017-02-02 | 2024-04-16 | 纽约基因组研究中心公司 | 用于识别或量化在生物样品中的靶标的方法和组合物 |
| CN110446789A (zh) | 2017-03-24 | 2019-11-12 | 赛卢拉研究公司 | 用于多重态确定的合成多重态 |
| KR20190133711A (ko) | 2017-03-24 | 2019-12-03 | 싱가포르국립대학교 | 분자의 다중 검출 방법 |
| WO2018217862A1 (en) | 2017-05-23 | 2018-11-29 | Centrillion Technologies, Inc. | Methods for performing spatial profiling of biological molecules |
| KR102550778B1 (ko) | 2017-05-26 | 2023-07-03 | 에이비비트로 엘엘씨 | 고-처리량 폴리뉴클레오타이드 라이브러리 시퀀싱 및 전사체 분석 |
| US10400235B2 (en) | 2017-05-26 | 2019-09-03 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| MX2019014264A (es) | 2017-05-29 | 2020-01-23 | Harvard College | Un metodo de amplificacion de transcriptoma unicelular. |
| CN110719959B (zh) | 2017-06-05 | 2021-08-06 | 贝克顿迪金森公司 | 针对单细胞的样品索引 |
| US20200217850A1 (en) | 2017-09-15 | 2020-07-09 | Apton Biosystems, Inc. | Heterogeneous single cell profiling using molecular barcoding |
| CN111247589B (zh) | 2017-09-25 | 2025-06-03 | 贝克顿迪金森公司 | 免疫受体条形码错误校正 |
| WO2019076768A1 (en) | 2017-10-16 | 2019-04-25 | Tervisetehnoloogiate Arenduskeskus As | METHOD AND KIT FOR PREPARING DNA BANK |
| WO2019084046A1 (en) | 2017-10-23 | 2019-05-02 | The Broad Institute, Inc. | ENRICHING SINGLE CELL CELLULAR CONSTITUENT OF BARCODE SEQUENCING LIBRARY |
| US20200354767A1 (en) | 2017-11-16 | 2020-11-12 | The Regents Of The University Of Colorado, A Body Corporate | Methods to measure functional heterogeneity among single cells |
| US20200362334A1 (en) | 2017-12-07 | 2020-11-19 | The Broad Institute, Inc. | High-throughput methods for identifying gene interactions and networks |
| WO2019113506A1 (en) | 2017-12-07 | 2019-06-13 | The Broad Institute, Inc. | Methods and compositions for multiplexing single cell and single nuclei sequencing |
| JP2021505157A (ja) | 2017-12-07 | 2021-02-18 | マサチューセッツ インスティテュート オブ テクノロジー | 単一細胞分析 |
| EP3568494B1 (en) | 2017-12-08 | 2021-05-05 | 10X Genomics, Inc. | Methods and compositions for labeling cells |
| EP3724658A1 (en) | 2017-12-12 | 2020-10-21 | 10X Genomics, Inc. | Systems and methods for single cell processing |
| CN111712579B (zh) | 2017-12-22 | 2024-10-15 | 10X基因组学有限公司 | 用于处理来自一个或多个细胞的核酸分子的系统和方法 |
| EP4650456A3 (en) | 2018-01-05 | 2026-01-21 | BillionToOne, Inc. | Quality control templates for ensuring validity of sequencing-based assays |
| SG11202007686VA (en) | 2018-02-12 | 2020-09-29 | 10X Genomics Inc | Methods characterizing multiple analytes from individual cells or cell populations |
| US12103004B2 (en) | 2018-03-12 | 2024-10-01 | Silicon Valley Scientific, Inc. | Method and apparatus for processing tissue and other samples encoding cellular spatial position information |
| EP3784797A1 (en) | 2018-04-27 | 2021-03-03 | X Gen US Co. | Methods and compositions for preparing polynucleotides |
| JP7358388B2 (ja) | 2018-05-03 | 2023-10-10 | ベクトン・ディキンソン・アンド・カンパニー | 反対側の転写物末端における分子バーコーディング |
| CN112272710A (zh) | 2018-05-03 | 2021-01-26 | 贝克顿迪金森公司 | 高通量多组学样品分析 |
| CN112840024A (zh) | 2018-08-03 | 2021-05-25 | 贝克顿迪金森公司 | 单细胞中的核条形码化和捕获 |
| US20210302422A1 (en) | 2018-08-08 | 2021-09-30 | Vanderbilt University | Systems and methods for simultaneous detection of antigens and antigen specific antibodies |
| JP7590955B2 (ja) | 2018-08-17 | 2024-11-27 | ベクトン・ディキンソン・アンド・カンパニー | アプタマーバーコーディング |
| CN112912513A (zh) | 2018-08-28 | 2021-06-04 | 贝克顿迪金森公司 | 使用糖类结合试剂和膜透过性试剂进行样品多重化 |
| ES2992135T3 (es) | 2018-10-01 | 2024-12-09 | Becton Dickinson Co | Determinar secuencias de transcripción 5 |
| US11932849B2 (en) | 2018-11-08 | 2024-03-19 | Becton, Dickinson And Company | Whole transcriptome analysis of single cells using random priming |
| US11492660B2 (en) | 2018-12-13 | 2022-11-08 | Becton, Dickinson And Company | Selective extension in single cell whole transcriptome analysis |
| ES2945227T3 (es) | 2019-01-23 | 2023-06-29 | Becton Dickinson Co | Oligonucleótidos asociados con anticuerpos |
| WO2020167920A1 (en) | 2019-02-14 | 2020-08-20 | Cellular Research, Inc. | Hybrid targeted and whole transcriptome amplification |
| US11965208B2 (en) | 2019-04-19 | 2024-04-23 | Becton, Dickinson And Company | Methods of associating phenotypical data and single cell sequencing data |
| WO2021146219A1 (en) | 2020-01-13 | 2021-07-22 | Becton, Dickinson And Company | Cell capture using du-containing oligonucleotides |
| US11649497B2 (en) | 2020-01-13 | 2023-05-16 | Becton, Dickinson And Company | Methods and compositions for quantitation of proteins and RNA |
| WO2021146636A1 (en) | 2020-01-17 | 2021-07-22 | Becton, Dickinson And Company | Methods and compositions for single cell secretomics |
| WO2021155057A1 (en) | 2020-01-29 | 2021-08-05 | Becton, Dickinson And Company | Barcoded wells for spatial mapping of single cells through sequencing |
| CN115427584A (zh) | 2020-01-31 | 2022-12-02 | 贝克顿迪金森公司 | 嗜中温dna聚合酶延伸阻断物 |
| US20210246492A1 (en) | 2020-02-12 | 2021-08-12 | Becton, Dickinson And Company | Intracellular abseq |
| WO2021173719A1 (en) | 2020-02-25 | 2021-09-02 | Becton, Dickinson And Company | Bi-specific probes to enable the use of single-cell samples as single color compensation control |
| US11661625B2 (en) | 2020-05-14 | 2023-05-30 | Becton, Dickinson And Company | Primers for immune repertoire profiling |
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