EP3041957A4 - Proximity assays for detecting nucleic acids and proteins in a single cell - Google Patents

Proximity assays for detecting nucleic acids and proteins in a single cell Download PDF

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Publication number
EP3041957A4
EP3041957A4 EP14842635.6A EP14842635A EP3041957A4 EP 3041957 A4 EP3041957 A4 EP 3041957A4 EP 14842635 A EP14842635 A EP 14842635A EP 3041957 A4 EP3041957 A4 EP 3041957A4
Authority
EP
European Patent Office
Prior art keywords
proteins
nucleic acids
single cell
detecting nucleic
proximity assays
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP14842635.6A
Other languages
German (de)
French (fr)
Other versions
EP3041957A1 (en
Inventor
Camila EGIDIO
Ramesh Ramakrishnan
David Ruff
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Standard Biotools Corp
Original Assignee
Fluidigm Corp
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Filing date
Publication date
Application filed by Fluidigm Corp filed Critical Fluidigm Corp
Publication of EP3041957A1 publication Critical patent/EP3041957A1/en
Publication of EP3041957A4 publication Critical patent/EP3041957A4/en
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP14842635.6A 2013-09-04 2014-09-04 Proximity assays for detecting nucleic acids and proteins in a single cell Withdrawn EP3041957A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361873820P 2013-09-04 2013-09-04
US201461987401P 2014-05-01 2014-05-01
PCT/US2014/054146 WO2015035087A1 (en) 2013-09-04 2014-09-04 Proximity assays for detecting nucleic acids and proteins in a single cell

Publications (2)

Publication Number Publication Date
EP3041957A1 EP3041957A1 (en) 2016-07-13
EP3041957A4 true EP3041957A4 (en) 2017-03-29

Family

ID=52628934

Family Applications (1)

Application Number Title Priority Date Filing Date
EP14842635.6A Withdrawn EP3041957A4 (en) 2013-09-04 2014-09-04 Proximity assays for detecting nucleic acids and proteins in a single cell

Country Status (6)

Country Link
US (2) US20150132743A1 (en)
EP (1) EP3041957A4 (en)
CN (1) CN105683397A (en)
CA (1) CA2922926A1 (en)
SG (1) SG10201908167YA (en)
WO (1) WO2015035087A1 (en)

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CN108693144B (en) * 2018-04-28 2021-02-09 天津大学 Multiplexing single-cell proteomics monitoring method based on SPRM technology
EP4234717A3 (en) 2018-05-03 2023-11-01 Becton, Dickinson and Company High throughput multiomics sample analysis
JP7358388B2 (en) 2018-05-03 2023-10-10 ベクトン・ディキンソン・アンド・カンパニー Molecular barcoding at opposite transcript ends
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CN113195717A (en) 2018-12-13 2021-07-30 贝克顿迪金森公司 Selective extension in single cell whole transcriptome analysis
WO2020150356A1 (en) 2019-01-16 2020-07-23 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
CN113574178B (en) 2019-01-23 2024-10-29 贝克顿迪金森公司 Oligonucleotides associated with antibodies
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US11965208B2 (en) 2019-04-19 2024-04-23 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
US11939622B2 (en) 2019-07-22 2024-03-26 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
CN114568027A (en) * 2019-08-05 2022-05-31 使命生物公司 Method and apparatus for single cell analysis for determining cell trajectories
JP7522189B2 (en) 2019-11-08 2024-07-24 ベクトン・ディキンソン・アンド・カンパニー Use of Random Priming to Obtain Full-Length V(D)J Information for Immune Repertoire Sequencing
CN115244184A (en) 2020-01-13 2022-10-25 贝克顿迪金森公司 Methods and compositions for quantifying protein and RNA
JP2023514388A (en) 2020-02-20 2023-04-05 スタンダード バイオツールズ インコーポレイテッド Parallelized sample processing and library preparation
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Also Published As

Publication number Publication date
US20150132743A1 (en) 2015-05-14
CN105683397A (en) 2016-06-15
SG10201908167YA (en) 2019-10-30
WO2015035087A1 (en) 2015-03-12
EP3041957A1 (en) 2016-07-13
CA2922926A1 (en) 2015-03-12
US20190292581A1 (en) 2019-09-26

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