ES2250544T3 - Procedimiento para la purificacion de proteinas de interferon por cromatografia de intercambio cationico. - Google Patents
Procedimiento para la purificacion de proteinas de interferon por cromatografia de intercambio cationico.Info
- Publication number
- ES2250544T3 ES2250544T3 ES02013555T ES02013555T ES2250544T3 ES 2250544 T3 ES2250544 T3 ES 2250544T3 ES 02013555 T ES02013555 T ES 02013555T ES 02013555 T ES02013555 T ES 02013555T ES 2250544 T3 ES2250544 T3 ES 2250544T3
- Authority
- ES
- Spain
- Prior art keywords
- proteins
- purification
- protein
- interferon
- cation exchange
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 125000005498 phthalate group Chemical class 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229920001467 poly(styrenesulfonates) Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- IWZKICVEHNUQTL-UHFFFAOYSA-M potassium hydrogen phthalate Chemical compound [K+].OC(=O)C1=CC=CC=C1C([O-])=O IWZKICVEHNUQTL-UHFFFAOYSA-M 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 238000000751 protein extraction Methods 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- -1 salts inorganic Chemical class 0.000 description 1
- 239000012047 saturated solution Substances 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 229940083608 sodium hydroxide Drugs 0.000 description 1
- 235000011121 sodium hydroxide Nutrition 0.000 description 1
- RGHFKWPGWBFQLN-UHFFFAOYSA-M sodium;5,5-diethylpyrimidin-3-ide-2,4,6-trione Chemical compound [Na+].CCC1(CC)C([O-])=NC(=O)NC1=O RGHFKWPGWBFQLN-UHFFFAOYSA-M 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000001384 succinic acid Substances 0.000 description 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 238000000825 ultraviolet detection Methods 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 238000012784 weak cation exchange Methods 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 150000003754 zirconium Chemical class 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/76—Albumins
- C07K14/765—Serum albumin, e.g. HSA
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/56—IFN-alpha
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/811—Interferon
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Virology (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ITBO01A0426 | 2001-07-06 | ||
| IT2001BO000426A ITBO20010426A1 (it) | 2001-07-06 | 2001-07-06 | Processo per la purificazione di proteine farmacologicamente attive mediante cromatografia in scambio cationico |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2250544T3 true ES2250544T3 (es) | 2006-04-16 |
Family
ID=11439471
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES02013555T Expired - Lifetime ES2250544T3 (es) | 2001-07-06 | 2002-06-19 | Procedimiento para la purificacion de proteinas de interferon por cromatografia de intercambio cationico. |
Country Status (27)
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|---|---|
| US (1) | US6866782B2 (enExample) |
| EP (1) | EP1273592B1 (enExample) |
| JP (1) | JP3940037B2 (enExample) |
| KR (1) | KR100771252B1 (enExample) |
| CN (1) | CN100484954C (enExample) |
| AR (1) | AR034663A1 (enExample) |
| AT (1) | ATE307824T1 (enExample) |
| BG (1) | BG65748B1 (enExample) |
| BR (1) | BR0202431A (enExample) |
| CA (1) | CA2388716C (enExample) |
| CU (1) | CU23149A3 (enExample) |
| DE (1) | DE60206847T2 (enExample) |
| DK (1) | DK1273592T3 (enExample) |
| DZ (1) | DZ3236A1 (enExample) |
| EG (1) | EG23150A (enExample) |
| ES (1) | ES2250544T3 (enExample) |
| HR (1) | HRP20020480B1 (enExample) |
| HU (1) | HUP0202124A2 (enExample) |
| IT (1) | ITBO20010426A1 (enExample) |
| ME (1) | MEP3408A (enExample) |
| MX (1) | MXPA02006303A (enExample) |
| PL (1) | PL209954B1 (enExample) |
| RS (1) | RS51512B (enExample) |
| RU (1) | RU2274471C2 (enExample) |
| SI (1) | SI1273592T1 (enExample) |
| SK (1) | SK287827B6 (enExample) |
| UY (1) | UY27354A1 (enExample) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1628729A2 (en) * | 2003-05-16 | 2006-03-01 | Cryobiophysica, Inc. | External gradient chromatofocusing |
| WO2005100379A1 (ja) * | 2004-03-30 | 2005-10-27 | Hiroshi Yanagisawa | タンパク質の分離方法 |
| US7449116B2 (en) * | 2004-10-01 | 2008-11-11 | Agilent Technologies, Inc. | Methods and systems for protein separation |
| US7943046B2 (en) * | 2004-10-01 | 2011-05-17 | Agilent Technologies, Inc | Methods and systems for on-column protein delipidation |
| JP5033177B2 (ja) * | 2006-04-12 | 2012-09-26 | サビエント ファーマセウティカルズ インク． | 陽イオン界面活性剤によるタンパク質の精製 |
| US20100261275A1 (en) * | 2007-12-10 | 2010-10-14 | Yves Durocher | Production of Recombinant Interferon Proteins |
| DE102009032179A1 (de) | 2009-07-07 | 2011-01-13 | Biogenerix Ag | Verfahren zur Reinigung von Interferon beta |
| CN101768601B (zh) * | 2010-02-01 | 2013-08-07 | 山东泉港药业有限公司 | 一种重组人血白蛋白—干扰素α2b的生产方法 |
| SG10201605397PA (en) * | 2011-07-01 | 2016-08-30 | Hoffmann La Roche | Method For Separation Of Monomeric Polypeptides From Aggregated Polypeptides |
| US8921113B2 (en) | 2012-12-21 | 2014-12-30 | Dionex Corporation | Buffer kit and method of generating a linear pH gradient |
| EP2964663A2 (en) * | 2013-03-08 | 2016-01-13 | Genzyme Corporation | Continuous purification of therapeutic proteins |
| CN104236983A (zh) * | 2013-06-14 | 2014-12-24 | 中国科学院大连化学物理研究所 | 溶液样品中含长烷基链的离子液体的去除方法 |
| TWI671312B (zh) | 2014-01-17 | 2019-09-11 | 美商健臻公司 | 無菌層析法及製法 |
| TWI709569B (zh) | 2014-01-17 | 2020-11-11 | 美商健臻公司 | 無菌層析樹脂及其用於製造方法的用途 |
| EP3221450A1 (en) * | 2014-11-18 | 2017-09-27 | Unichem Laboratories Limited | An improved process for the preparation of pharmacopoeial grade interferon alpha 2b |
| CN106496302B (zh) * | 2015-09-08 | 2021-12-10 | 三生国健药业（上海）股份有限公司 | 一种用离子交换层析纯化蛋白质的方法 |
| GB201517450D0 (en) * | 2015-10-02 | 2015-11-18 | Ipsen Biopharm Ltd | Method |
| EP4218992A3 (en) * | 2015-12-09 | 2023-08-09 | Basf Se | Method of purifying a protein from fermentation solids under desorbing conditions |
| GB2568936B (en) | 2017-12-01 | 2019-12-25 | Ford Global Tech Llc | Liquid cooled injector |
| CA3110666A1 (en) | 2018-08-31 | 2020-03-05 | Genzyme Corporation | Sterile chromatography resin and use thereof in manufacturing processes |
| US11022585B2 (en) | 2019-06-09 | 2021-06-01 | Dionex Corporation | Methods and systems for optimizing buffer conditions with liquid chromatography |
| CN111138523A (zh) * | 2019-12-10 | 2020-05-12 | 天津生机集团股份有限公司 | 一种从重组鸡干扰素α复性液中纯化制备重组鸡干扰素α的方法 |
| CN113968900B (zh) * | 2020-07-23 | 2023-07-25 | 中元汇吉生物技术股份有限公司 | 一种纯化C1q蛋白的方法 |
| CN115918902A (zh) * | 2022-12-19 | 2023-04-07 | 新疆农业科学院农业质量标准与检测技术研究所 | 一种通过微水波扩散重力法预处理葡萄脱水并获取高酚葡萄提取物的方法 |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1617401A1 (de) * | 1966-05-02 | 1972-02-17 | Centre Nat Rech Scient | Verfahren zur Herstellung von Interferon-Praeparaten |
| US4228154A (en) * | 1979-02-26 | 1980-10-14 | Armour Pharmaceutical Company | Purification of plasma albumin by ion exchange chromatography |
| US4534906A (en) * | 1982-11-01 | 1985-08-13 | Genentech, Inc. | Removal of impurities from human leukocyte interferon preparations |
| US5196323A (en) * | 1985-04-27 | 1993-03-23 | Boehringer Ingelheim International Gmbh | Process for preparing and purifying alpha-interferon |
| DE3515336C2 (de) * | 1985-04-27 | 1994-01-20 | Boehringer Ingelheim Int | Verfahren zur Herstellung und Reinigung von â-Interferon |
| US5037644A (en) * | 1986-10-27 | 1991-08-06 | Cetus Corporation | Pharmaceutical compositions of recombinant interleukin-2 and formulation processes |
| US4732683A (en) * | 1986-12-02 | 1988-03-22 | Biospectrum, Inc. | Purification method for alpha interferon |
| US4765903A (en) * | 1987-10-06 | 1988-08-23 | Interferon Sciences, Inc. | Purification of monomeric interferon |
| US5250662A (en) * | 1989-10-05 | 1993-10-05 | Alpha Therapeutic Corporation | Albumin purification |
| US5849874A (en) * | 1991-07-12 | 1998-12-15 | Gist-Brocades, N.V. | Process for the purification of serum albumin |
| IT1262899B (it) * | 1992-03-27 | 1996-07-22 | Sclavo Spa | Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano |
| US5440018A (en) * | 1992-05-20 | 1995-08-08 | The Green Cross Corporation | Recombinant human serum albumin, process for producing the same and pharmaceutical preparation containing the same |
| US5521287A (en) * | 1992-05-20 | 1996-05-28 | The Green Cross Corporation | Recombinant human serum albumin, process for producing the same and pharmaceutical preparation containing the same |
| US5728553A (en) * | 1992-09-23 | 1998-03-17 | Delta Biotechnology Limited | High purity albumin and method of producing |
| US5367054A (en) * | 1993-04-12 | 1994-11-22 | Stolle Research & Development Corp. | Large-scale purification of egg immunoglobulin |
| DE69522492T2 (de) * | 1994-04-09 | 2002-05-23 | F. Hoffmann-La Roche Ag, Basel | Verfahren zur Herstellung von Alpha-Interferon |
| JP3702474B2 (ja) * | 1994-06-01 | 2005-10-05 | 三菱ウェルファーマ株式会社 | 血清アルブミン製剤の製造方法 |
| RU2081122C1 (ru) * | 1994-06-16 | 1997-06-10 | Институт биоорганической химии им.М.М.Шемякина и Ю.А.Овчинникова РАН | Способ выделения и очистки инсулина или его биотехнологических предшественников |
| US5486470A (en) * | 1994-07-21 | 1996-01-23 | Merck & Co., Inc. | Purified herpes simplex virus protease and methods of purification |
| CA2157219C (en) | 1994-08-31 | 2010-10-05 | Munehiro Noda | Process for purifying recombinant human serum albumin |
| EP1736483A3 (en) * | 1995-09-07 | 2008-07-30 | Pharming Intellectual Property BV | Purification of alpha-1 proteinase inhibitor |
| RU2132386C1 (ru) * | 1997-06-20 | 1999-06-27 | Государственный научный центр вирусологии и биотехнологии "Вектор" | Способ получения рекомбинантного интерферона-гамма человека |
| GB9902000D0 (en) * | 1999-01-30 | 1999-03-17 | Delta Biotechnology Ltd | Process |
-
2001
- 2001-07-06 IT IT2001BO000426A patent/ITBO20010426A1/it unknown
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2002
- 2002-05-31 HR HR20020480A patent/HRP20020480B1/xx not_active IP Right Cessation
- 2002-06-03 BG BG106768A patent/BG65748B1/bg unknown
- 2002-06-03 CA CA002388716A patent/CA2388716C/en not_active Expired - Fee Related
- 2002-06-06 CU CU112A patent/CU23149A3/es not_active IP Right Cessation
- 2002-06-10 US US10/166,459 patent/US6866782B2/en not_active Expired - Fee Related
- 2002-06-14 SK SK859-2002A patent/SK287827B6/sk not_active IP Right Cessation
- 2002-06-19 SI SI200230227T patent/SI1273592T1/sl unknown
- 2002-06-19 EP EP02013555A patent/EP1273592B1/en not_active Expired - Lifetime
- 2002-06-19 AT AT02013555T patent/ATE307824T1/de active
- 2002-06-19 DK DK02013555T patent/DK1273592T3/da active
- 2002-06-19 ES ES02013555T patent/ES2250544T3/es not_active Expired - Lifetime
- 2002-06-19 DE DE60206847T patent/DE60206847T2/de not_active Expired - Lifetime
- 2002-06-24 MX MXPA02006303A patent/MXPA02006303A/es active IP Right Grant
- 2002-06-24 UY UY27354A patent/UY27354A1/es not_active Application Discontinuation
- 2002-06-26 DZ DZ020153A patent/DZ3236A1/fr active
- 2002-06-26 RS YUP-505/02A patent/RS51512B/sr unknown
- 2002-06-26 ME MEP-34/08A patent/MEP3408A/xx unknown
- 2002-06-26 JP JP2002186407A patent/JP3940037B2/ja not_active Expired - Fee Related
- 2002-06-27 BR BR0202431-4A patent/BR0202431A/pt not_active Application Discontinuation
- 2002-06-27 PL PL354769A patent/PL209954B1/pl unknown
- 2002-06-28 CN CNB021402175A patent/CN100484954C/zh not_active Expired - Fee Related
- 2002-06-28 RU RU2002117383/15A patent/RU2274471C2/ru not_active IP Right Cessation
- 2002-06-28 AR ARP020102459A patent/AR034663A1/es active IP Right Grant
- 2002-06-28 HU HU0202124A patent/HUP0202124A2/hu not_active Application Discontinuation
- 2002-06-29 KR KR1020020037622A patent/KR100771252B1/ko not_active Expired - Fee Related
- 2002-06-29 EG EG2002060743A patent/EG23150A/xx active
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